README.md

---
title: AdaptiveDNA API
emoji: 🧬
colorFrom: green
colorTo: blue
sdk: docker
app_port: 7860
pinned: false
license: mit
---

# AdaptiveDNA API

CRISPR guide RNA design and DNA sequence analysis API for the AdaptiveDNA GPT.

## Endpoints

| Method | Path | Description |
|--------|------|-------------|
| POST | `/analyze_sequence` | Sequence statistics (GC%, Tm, nucleotide counts) |
| POST | `/design_guide_rna` | Ranked CRISPR guide RNA candidates |
| POST | `/find_crop_targets` | Gene-editing targets for Rice/Maize/Wheat/Tomato/Soybean |
| POST | `/calculate_gc` | Quick GC content |
| GET  | `/nuclease_presets` | SpCas9, SaCas9, Cpf1/Cas12a, CjCas9 specs |
| GET  | `/crops` | Supported crop list |
| POST | `/restriction_sites` | Restriction enzyme site finder |
| GET  | `/health` | Liveness check |

Interactive docs at `/docs`.