Update app.py

app.py

@@ -25,7 +25,6 @@ def extract_features_from_genbank(genbank_content, feature_types=['CDS', 'tRNA',
 
 def design_primers_for_region(sequence, product_size_range, num_to_return=10):
     """Design primers for a specific sequence."""
-    # Parse the product size range
     size_min, size_max = map(int, product_size_range.split('-'))
     return primer3.bindings.designPrimers(
         {
@@ -59,15 +58,13 @@ if uploaded_file is not None:
     st.text(str(feature_sequence))
 
     product_size_range = st.text_input("Enter desired PCR product size range (e.g., 150-500):", value="150-500")
-
-    # Let users specify the minimum number of primer pairs to return, ensuring it's at least 5
     min_num_primers = st.number_input("Enter minimum number of primer pairs to return:", min_value=5, value=5, step=1)
 
     if st.button(f'Design Primers for selected {feature_type}'):
         primers = design_primers_for_region(feature_sequence, product_size_range, num_to_return=min_num_primers)
         
         primer_data = []
-        for i in range(min_num_primers):  # Collect data for the specified number of primer pairs
+        for i in range(min_num_primers):
             left_sequence = primers.get(f'PRIMER_LEFT_{i}_SEQUENCE', 'N/A')
             right_sequence = primers.get(f'PRIMER_RIGHT_{i}_SEQUENCE', 'N/A')
             if left_sequence != 'N/A' and right_sequence != 'N/A':
@@ -77,8 +74,8 @@ if uploaded_file is not None:
                     'Right Sequence': right_sequence,
                     'Left TM (°C)': primers.get(f'PRIMER_LEFT_{i}_TM', 'N/A'),
                     'Right TM (°C)': primers.get(f'PRIMER_RIGHT_{i}_TM', 'N/A'),
-                    'Left Length': primers.get(f'PRIMER_LEFT_{i}_SIZE', 'N/A'),
-                    'Right Length': primers.get(f'PRIMER_RIGHT_{i}_SIZE', 'N/A'),
+                    'Left Length': len(left_sequence),  # Get the length of the left primer
+                    'Right Length': len(right_sequence),  # Get the length of the right primer
                     'PCR Product Size (bp)': primers.get(f'PRIMER_PAIR_{i}_PRODUCT_SIZE', 'N/A')
                 }
                 primer_data.append(primer_info)