PANDA / scripts /hematopoiesis /README.md
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Correction pass: gate-matched Dahlin, retracted unsupported claims, complete HF-placode DEG set, restyled figures
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Pan-Hematopoiesis PANDA

Multi-dataset HSC + hematopoietic lineage corpus for cell identity + zero-shot transfer + mechanistic discovery.

Corpus datasets

Accession Study Cells Species Modality Role
GSE140802 Weinreb LARRY 2020 75,339 mouse 10x + clonal barcodes Anchor (local)
GSE72857 Paul 2015 ~10,000 mouse MARS-seq Foundational myeloid branch reference
GSE81682 Nestorowa 2016 ~1,656 mouse Smart-seq2 FACS-index-sorted HSPC anchor
GSE89754 Tusi 2018 ~4,763 mouse inDrops Erythroid trajectory + PBA fate probs
GSE107727 Dahlin 2018 ~44,802 mouse 10x Large LSK/Kit+ landscape + Kit-mutant contrast

Held-out labeled validation

Nestorowa 2016 index-sort labels — held-out from training and evaluated with the FACS-index-sorted ground truth (LT-HSC / MPP / GMP / MEP / CLP) which is orthogonal to mRNA. Provides a clean cross-lab / cross-platform accuracy measurement.

Held-out unlabeled discovery target

Dahlin 2018 W41-mutant subset — subset of GSE107727 where Kit-mutant hematopoiesis is stress-tested. Weakly labeled; PANDA predictions probe how mutation shifts the identity distribution.

Ontology (10 classes)

  • LT-HSC
  • MPP (multipotent progenitor)
  • CMP (common myeloid progenitor)
  • MEP (megakaryocyte-erythroid progenitor)
  • GMP (granulocyte-monocyte progenitor)
  • erythroid
  • myeloid (Neu + Mono + DC merged)
  • lymphoid (B/T/NK)
  • megakaryocyte
  • basophil-mast