PANDA / scripts /hematopoiesis /README.md
bryan7264's picture
Correction pass: gate-matched Dahlin, retracted unsupported claims, complete HF-placode DEG set, restyled figures
141bacd verified
|
Raw
History Blame Contribute Delete
1.55 kB
# Pan-Hematopoiesis PANDA
Multi-dataset HSC + hematopoietic lineage corpus for cell identity + zero-shot transfer +
mechanistic discovery.
## Corpus datasets
| Accession | Study | Cells | Species | Modality | Role |
|---|---|---:|---|---|---|
| GSE140802 | Weinreb LARRY 2020 | 75,339 | mouse | 10x + clonal barcodes | Anchor (local) |
| GSE72857 | Paul 2015 | ~10,000 | mouse | MARS-seq | Foundational myeloid branch reference |
| GSE81682 | Nestorowa 2016 | ~1,656 | mouse | Smart-seq2 | FACS-index-sorted HSPC anchor |
| GSE89754 | Tusi 2018 | ~4,763 | mouse | inDrops | Erythroid trajectory + PBA fate probs |
| GSE107727 | Dahlin 2018 | ~44,802 | mouse | 10x | Large LSK/Kit+ landscape + Kit-mutant contrast |
## Held-out labeled validation
**Nestorowa 2016 index-sort labels** — held-out from training and evaluated with the
FACS-index-sorted ground truth (LT-HSC / MPP / GMP / MEP / CLP) which is orthogonal to
mRNA. Provides a clean cross-lab / cross-platform accuracy measurement.
## Held-out unlabeled discovery target
**Dahlin 2018 W41-mutant subset** — subset of GSE107727 where Kit-mutant hematopoiesis is
stress-tested. Weakly labeled; PANDA predictions probe how mutation shifts the identity
distribution.
## Ontology (10 classes)
- LT-HSC
- MPP (multipotent progenitor)
- CMP (common myeloid progenitor)
- MEP (megakaryocyte-erythroid progenitor)
- GMP (granulocyte-monocyte progenitor)
- erythroid
- myeloid (Neu + Mono + DC merged)
- lymphoid (B/T/NK)
- megakaryocyte
- basophil-mast