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IL-33 is a tissue-derived cytokine that induces and amplifies eosinophilic inflammation and has emerged as a promising new drug target for asthma and allergic disease . Common variants at IL33 and IL1RL1 , encoding the IL-33 receptor ST2 , associate with eosinophil counts and asthma . Through whole-genome sequencing an...
Only a few genes have been found to play a role in asthma . These include the genes IL33 and IL1RL1 , and sequence variants in the human genome close to these genes were initially found to affect the number of eosinophils , cells that play a role in inflammation of the airways in asthma . Based on this knowledge , we d...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "sequencing", "techniques", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "cytokines", "pathology", "and", "laboratory", "medicine", "immunology", "messenger", "rna", "alleles", "pul...
2017
A rare IL33 loss-of-function mutation reduces blood eosinophil counts and protects from asthma
Immune responses and DNA damage repair are two fundamental processes that have been characterized extensively , but the links between them remain largely unknown . We report that multiple bacterial , fungal and oomycete plant pathogen species induce double-strand breaks ( DSBs ) in host plant DNA . DNA damage detected ...
Multicellular organisms are continuously exposed to microbes and have developed sophisticated defense mechanisms to counter attack by microbial pathogens . Organisms also encounter many types of DNA damage and have evolved multiple mechanisms to maintain their genomic integrity . Even though these two fundamental respo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "innate", "immune", "system", "cellular", "stress", "responses", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "cell", "processes", "immunology", "microbiology", "brassica", "plant", "science", "model", "organisms", "plant", "p...
2014
Microbial Pathogens Trigger Host DNA Double-Strand Breaks Whose Abundance Is Reduced by Plant Defense Responses
The evolutionary transition from outcrossing to self-fertilization ( selfing ) through the loss of self-incompatibility ( SI ) is one of the most prevalent events in flowering plants , and its genetic basis has been a major focus in evolutionary biology . In the Brassicaceae , the SI system consists of male and female ...
Since Charles Darwin recognized the extraordinary diversity of plant mating systems , deciphering their origins has been a central theme for evolutionary biologists . Among various sexual systems , the evolution of self-fertilization ( selfing ) from cross-fertilization is one of the most frequent transitions in flower...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "mutation", "genetic", "polymorphism", "genetics", "plant", "genetics", "population", "genetics", "biology", "evolutionary", "biology", "ploidy", "evolutionary", "genetics", "genetics", "and", "genomics", "gene", "function" ]
2012
Recent Loss of Self-Incompatibility by Degradation of the Male Component in Allotetraploid Arabidopsis kamchatica
B cell follicles in secondary lymphoid tissues represent an immune privileged sanctuary for AIDS viruses , in part because cytotoxic CD8+ T cells are mostly excluded from entering the follicles that harbor infected T follicular helper ( TFH ) cells . We studied the effects of native heterodimeric IL-15 ( hetIL-15 ) tre...
Heterodimeric interleukin-15 ( hetIL-15 ) , the stable native complex of IL-15 and IL-15 Receptor α ( IL-15/IL-15Rα ) , activates and expands cytotoxic T and NK cells . Based on these properties , hetIL-15 is currently being evaluated in clinical trials in advanced cancer . To explore potential utility in AIDS virus in...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "flow", "cytometry", "medicine", "and", "health", "sciences", "immune", "cells", "immunology", "vertebrates", "cloning", "animals", "mammals", "primates", "cytotoxic", "t", "cells", "molecular", "biology", "techniques", "old", "world", "monkeys", "...
2018
Treatment with native heterodimeric IL-15 increases cytotoxic lymphocytes and reduces SHIV RNA in lymph nodes
Melioidosis , caused by the flagellated bacterium Burkholderia pseudomallei , is a life-threatening and increasingly recognized emerging disease . Toll-like receptor ( TLR ) 5 is a germline-encoded pattern recognition receptor to bacterial flagellin . We evaluated the association of a nonsense TLR5 genetic variant that...
Melioidosis is a high-mortality infectious disease in Southeast Asia and northern Australia caused by Burkholderia pseudomallei , which is a flagellated , rod-shaped Gram-negative bacterium . Understanding protective host immune responses to melioidosis is fundamental for effective vaccine development . A previous stud...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "blood", "cells", "innate", "immune", "system", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "immune", "cells", "body", "fluids", "immune", "physiology", "cytokines", "melioidosis", "immunology", "bacterial", "diseases", "developmental", "bi...
2017
A nonsense mutation in TLR5 is associated with survival and reduced IL-10 and TNF-α levels in human melioidosis
The cerebral cortex performs complex cognitive functions at the expense of tremendous energy consumption . Blood vessels in the brain are known to form stereotypic patterns that facilitate efficient oxygen and nutrient delivery . Yet little is known about how vessel development in the brain is normally regulated . Radi...
The brain is an energy-intensive organ that consumes about 10 times as much energy per unit volume as the rest of the body . It therefore requires a highly efficient vascular network for oxygen and nutrient delivery , and as a result compromises in blood vessel networks influence a wide array of brain diseases . Our cu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neurobiology", "of", "disease", "and", "regeneration", "neurogenesis", "cardiovascular", "anatomy", "anatomy", "and", "physiology", "neuroscience", "neuroglial", "development", "developmental", "biology", "organism", "development", "stem", "cells", "molecular", "developmen...
2013
Radial Glial Neural Progenitors Regulate Nascent Brain Vascular Network Stabilization Via Inhibition of Wnt Signaling
The fibrinogen ( Fg ) binding MSCRAMM Clumping factor A ( ClfA ) from Staphylococcus aureus interacts with the C-terminal region of the fibrinogen ( Fg ) γ-chain . ClfA is the major virulence factor responsible for the observed clumping of S . aureus in blood plasma and has been implicated as a virulence factor in a mo...
Staphylococcus aureus ( S . aureus ) is a common pathogen that can cause a range of diseases from mild skin infections to life-threatening sepsis in humans . Some surface proteins on S . aureus play important roles in the S . aureus disease process . One of these bacterial surface proteins is clumping factor A ( ClfA )...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/nosocomial", "and", "healthcare-associated", "infections", "biophysics/biomacromolecule-ligand", "interactions", "biochemistry/biomacromolecule-ligand", "interactions", "infectious", "diseases/bacterial", "infections", "microbiology/cellular", "microbiology", "and...
2008
A Structural Model of the Staphylococcus aureus ClfA–Fibrinogen Interaction Opens New Avenues for the Design of Anti-Staphylococcal Therapeutics
There is increasing evidence that heritable variation in gene expression underlies genetic variation in susceptibility to disease . Therefore , a comprehensive understanding of the similarity between relatives for transcript variation is warranted—in particular , dissection of phenotypic variation into additive and non...
Gene expression levels are known to influence common disease susceptibility in humans , with GWAS significant SNPs frequently found in regulatory regions . The expression levels of most genes are influenced by genetic variants , often located close to the gene itself . Expression Quantitative Trait Loci ( eQTL ) mappin...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome-wide", "association", "studies", "genome", "expression", "analysis", "genome", "analysis", "tools", "genome", "complexity", "genetic", "networks", "genome", "scans", "trait", "locus", "analysis", "biology", "genomics", "transcriptomes" ]
2013
Congruence of Additive and Non-Additive Effects on Gene Expression Estimated from Pedigree and SNP Data
The Gram-positive bacterium Staphylococcus aureus , similar to other pathogens , binds human complement regulators Factor H and Factor H related protein 1 ( FHR-1 ) from human serum . Here we identify the secreted protein Sbi ( Staphylococcus aureus binder of IgG ) as a ligand that interacts with Factor H by a—to our k...
Staphylococcus aureus is a Gram-positive bacterium that can live as a commensal but can also cause severe life threatening infections in humans . Upon infection the bacterium is attacked by the host immune system , and in particular by the complement system which forms the immediate , first defence line of innate immun...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/immune", "response", "immunology/innate", "immunity", "microbiology", "infectious", "diseases/bacterial", "infections", "immunology/immunity", "to", "infections" ]
2008
The Staphylococcus aureus Protein Sbi Acts as a Complement Inhibitor and Forms a Tripartite Complex with Host Complement Factor H and C3b
Kaposi’s sarcoma-associated herpesvirus ( KSHV ) is an oncogenic herpesvirus associated with various AIDS-related malignancies . Like other herpesviruses , multiple processes required for KSHV lytic replication , including viral transcription , viral DNA synthesis and capsid assembly occur in virus-induced intranuclear...
Molecular chaperones from the HSP70 and HSP90 families have important roles in cell survival . Recent evidence has also implicated their functioning in a variety of diseases , including cancer . As such they have been identified as emerging drug targets . Kaposi’s sarcoma-associated herpesvirus ( KSHV ) is an oncogenic...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Hsp70 Isoforms Are Essential for the Formation of Kaposi’s Sarcoma-Associated Herpesvirus Replication and Transcription Compartments
The lipid composition of cell membranes has increasingly been recognized as playing an important role in the function of various membrane proteins , including G Protein-Coupled Receptors ( GPCRs ) . For instance , experimental and computational evidence has pointed to lipids influencing receptor oligomerization directl...
The μ-opioid receptor ( MOR ) is an important pharmaceutical target in the treatment of pain . In order to develop novel pain therapies , devoid of the serious side-effects of present opioid analgesics , we need to understand the fundamentals of how MOR works on the molecular level . While some studies suggest that oli...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "crystal", "structure", "condensed", "matter", "physics", "membrane", "proteins", "membrane", "receptor", "signaling", "lipid", "signaling", "crystallography", "cellular", "structures", "and", "organelles", "g", "protein", "coupled", "receptors", "lipids", "solid", "sta...
2016
Impact of Lipid Composition and Receptor Conformation on the Spatio-temporal Organization of μ-Opioid Receptors in a Multi-component Plasma Membrane Model
Current sequencing methods produce large amounts of data , but genome assemblies based on these data are often woefully incomplete . These incomplete and error-filled assemblies result in many annotation errors , especially in the number of genes present in a genome . In this paper we investigate the magnitude of the p...
The initial publication of the genome sequence of many plants , animals , and microbes is often accompanied with great fanfare . However , these genomes are almost always first-drafts , with a lot of missing data , many gaps , and many errors in the published sequences . Compounding this problem , the genes identified ...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[ "sequencing", "techniques", "genome", "sequencing", "sequence", "analysis", "genomics", "genome", "evolution", "genetics", "biology", "and", "life", "sciences", "comparative", "genomics", "molecular", "biology", "techniques", "computational", "biology", "molecular", "biol...
2014
Extensive Error in the Number of Genes Inferred from Draft Genome Assemblies
Male mammals produce sperm for most of postnatal life and therefore require a robust germ line stem cell system , with precise balance between self-renewal and differentiation . Prior work established doublesex- and mab-3-related transcription factor 1 ( Dmrt1 ) as a conserved transcriptional regulator of male sexual d...
The Dmrt1 gene is a deeply conserved gonadal regulator that is expressed in all mitotic germ cells of the mouse , including spermatogonial stem cells ( SSCs ) . We previously showed that Dmrt1 controls the mitosis/meiosis switch in differentiating mouse spermatogonia . Here we have examined the role of Dmrt1 in undiffe...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "meiosis", "medicine", "and", "health", "sciences", "reproductive", "system", "nuclear", "staining", "cell", "cycle", "and", "cell", "division", "cell", "processes", "spermatogonia", "cell", "differentiation", "reproductive", "physiology", "germ", "cells", "developmenta...
2016
DMRT1 Is Required for Mouse Spermatogonial Stem Cell Maintenance and Replenishment
The control of cutaneous leishmaniasis ( CL ) is facilitated by knowledge of factors associated with the treatment failures in endemic countries . The aim of this evaluation was to identify the potential risk determinants which might affect the significance of demographic and clinical characteristics for the patients w...
Cutaneous leishmaniasis ( CL ) is a serious neglected tropical disease with social stigma and associated disfiguring with health burden , especially in poor endemic regions of the world . Iran is among the seven high burden CL-infected countries . Limited data are available in regarding to CL treatment and related risk...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "geographical", "locations", "parasitic", "diseases", "parasitic", "protozoans", "health", "care", "research", "design", "protozoans", "signs", "and", "symptom...
2019
Associated-risk determinants for anthroponotic cutaneous leishmaniasis treated with meglumine antimoniate: A cohort study in Iran
CTP synthase ( CTPsyn ) is essential for the biosynthesis of pyrimidine nucleotides . It has been shown that CTPsyn is incorporated into a novel cytoplasmic structure which has been termed the cytoophidium . Here , we report that Myc regulates cytoophidium formation during Drosophila oogenesis . We have found that Myc ...
The coordination of metabolism with cell growth is critical for regulation of organismal development . Therefore there is significant interplay between metabolic enzymes and key developmental regulators such as transcription factors . The enzyme CTP synthase ( CTPsyn ) is essential for metabolic homeostasis as well as ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "rna", "interference", "enzymology", "cloning", "animals", "reproductive", "physiology", "animal", "models", "drosophila", "melanogaster", "model", "organisms", "enzyme", "metabolism", "molecular", "biology", "tech...
2016
The Interplay between Myc and CTP Synthase in Drosophila
Hepatitis C virus ( HCV ) infection is a leading cause of chronic liver diseases and hepatocellular carcinoma ( HCC ) and Golgi protein 73 ( GP73 ) is a serum biomarker for liver diseases and HCC . However , the mechanism underlying GP73 regulates HCV infection is largely unknown . Here , we revealed that GP73 acts as ...
Golgi protein 73 ( GP73 ) is a serum biomarker for liver diseases and hepatocellular carcinoma ( HCC ) . In this study , the authors reveal that GP73 acts as a novel negative regulator of host innate immunity to facilitate hepatitis C virus ( HCV ) infection . GP73 expression is activated and correlated with IFN-β prod...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "reverse", "transcriptase-polymerase", "chain", "reaction", "innate", "immune", "system", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "affinity", "chromatography", "hepacivirus", "pathogens", "immunology", "carcinomas", "microbiolo...
2017
GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation
Mutations in transmembrane inner ear ( TMIE ) cause deafness in humans; previous studies suggest involvement in the mechano-electrical transduction ( MET ) complex in sensory hair cells , but TMIE’s precise role is unclear . In tmie zebrafish mutants , we observed that GFP-tagged Tmc1 and Tmc2b , which are subunits of ...
Hair cells mediate hearing and balance through the activity of a mechanosensitive channel in the cell membrane . The transmembrane inner ear ( TMIE ) protein is an essential component of the protein complex that gates this so-called mechanotransduction channel . While it is known that loss of TMIE results in deafness ,...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "fluorescence", "imaging", "fish", "medicine", "and", "health", "sciences", "laboratory", "equipment", "engineering", "and", "technology", "ears", "vertebrates", "animals", "animal", "models", "osteichthyes", "developmental", "biology", "otology", "inner", "ear", "model...
2019
Subunits of the mechano-electrical transduction channel, Tmc1/2b, require Tmie to localize in zebrafish sensory hair cells
Bacterial pathogenicity relies on a proficient metabolism and there is increasing evidence that metabolic adaptation to exploit host resources is a key property of infectious organisms . In many cases , colonization by the pathogen also implies an intensive multiplication and the necessity to produce a large array of v...
Metabolic versatility is a critical element for pathogen’s virulence and their ability to survive in the host . Beyond the necessity to collect resources during infection , pathogens face a resource allocation dilemma: they have to use nutritional resources to proliferate inside the host , and in the other hand they ne...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[ "bacteriology", "medicine", "and", "health", "sciences", "protein", "metabolism", "pathology", "and", "laboratory", "medicine", "pathogens", "metabolic", "networks", "microbiology", "plant", "science", "secretion", "systems", "network", "analysis", "plant", "pathology", ...
2016
A Resource Allocation Trade-Off between Virulence and Proliferation Drives Metabolic Versatility in the Plant Pathogen Ralstonia solanacearum
The advancement of high-throughput sequencing ( HTS ) technologies and the rapid development of numerous analysis algorithms and pipelines in this field has resulted in an unprecedentedly high demand for training scientists in HTS data analysis . Embarking on developing new training materials is challenging for many re...
In recent years , the advancement of high-throughput sequencing ( HTS ) and the rapid development of numerous analysis algorithms and pipelines in this field have resulted in an unprecedentedly high demand for training scientists in HTS data analysis . Generating effective training materials is time-consuming , and a l...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Conclusions" ]
[ "education", "engineering", "and", "technology", "sociology", "social", "sciences", "mathematics", "statistics", "(mathematics)", "genome", "analysis", "research", "and", "analysis", "methods", "computer", "and", "information", "sciences", "genomics", "bioinformatics", "w...
2016
Training in High-Throughput Sequencing: Common Guidelines to Enable Material Sharing, Dissemination, and Reusability
The increased transcription of the Cyp6g1 gene of Drosophila melanogaster , and consequent resistance to insecticides such as DDT , is a widely cited example of adaptation mediated by cis-regulatory change . A fragment of an Accord transposable element inserted upstream of the Cyp6g1 gene is causally associated with re...
The study of insecticide resistance has greatly enriched our understanding of the genetic basis of adaptation , because it represents some of the most intense selection pressures acting on any natural population of eukaryote . Thus it can inform us about the limits of natural selection , both in terms of the number and...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology/evolutionary", "and", "comparative", "genetics", "genetics", "and", "genomics/population", "genetics" ]
2010
Copy Number Variation and Transposable Elements Feature in Recent, Ongoing Adaptation at the Cyp6g1 Locus
The endosymbiotic bacteria Wolbachia pipientis ( wMel strain ) has been successfully established in several populations of Aedes aegypti , the primary dengue vector . The virulent Wolbachia strain wMelPop is known to cause several pathological impacts ( increased egg mortality , life shortening , etc . ) reducing overa...
Dengue is a leading cause of morbidity in the tropics . As a commercial vaccine is not available , control or modification of the mosquito vectors is employed to prevent transmission . Strains of the endosymbiotic bacteria Wolbachia affect the survival and ability of the dengue vector Aedes aegypti to transmit dengue ....
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Application of wMelPop Wolbachia Strain to Crash Local Populations of Aedes aegypti
Fear learning is highly adaptive if utilized in appropriate situations but can lead to generalized anxiety if applied too widely . A role of predictive cues in inhibiting fear generalization has been suggested by stress and fear learning studies , but the effects of partially predictive cues ( ambiguous cues ) and the ...
The ability to predict whether an experience will end favorably is critical for well-being . Cues associated with specific outcomes can aid in prediction , enabling adaptive behaviors , but cue-outcome relationships are often difficult to learn or inherently ambiguous . Human studies have suggested that the hippocampus...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "neurogenesis", "medicine", "and", "health", "sciences", "brain", "vertebrates", "social", "sciences", "mice", "neuroscience", "animals", "mammals", "fear", "animal", "behavior", "zoology", "developmental", "neuroscience", "emotions", "animal", "cells", "behavior", "fea...
2017
Ongoing neurogenesis in the adult dentate gyrus mediates behavioral responses to ambiguous threat cues
The neuraminidase inhibitor oseltamivir is currently used for treatment of patients infected with the pandemic A/H1N1 ( pH1N1 ) influenza virus , although drug-resistant mutants can emerge rapidly and possibly be transmitted . We describe the characteristics of a pair of oseltamivir-resistant and oseltamivir-susceptibl...
During the 2009 pandemic of the novel A/H1N1 ( pH1N1 ) virus , the World Health Organization recommended oseltamivir as first-line agent for treatment of patients with severe infections leading to hospitalization and for those with underlying diseases predisposing to pulmonary complications . Oseltamivir-resistant isol...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/viral", "infections", "virology/animal", "models", "of", "infection", "virology/antivirals,", "including", "modes", "of", "action", "and", "resistance" ]
2010
Oseltamivir-Resistant Pandemic A/H1N1 Virus Is as Virulent as Its Wild-Type Counterpart in Mice and Ferrets
Simultaneous electrical stimulation and recording using multi-electrode arrays can provide a valuable technique for studying circuit connectivity and engineering neural interfaces . However , interpreting these measurements is challenging because the spike sorting process ( identifying and segregating action potentials...
Simultaneous electrical stimulation and recording using multi-electrode arrays can provide a valuable technique for studying circuit connectivity and engineering neural interfaces . However , interpreting these recordings is challenging because the spike sorting process ( identifying and segregating action potentials a...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "biotechnology", "medicine", "and", "health", "sciences", "action", "potentials", "applied", "mathematics", "membrane", "potential", "ocular", "anatomy", "electrophysiology", "neuroscience", "surgical", "and", "invasive", "medical", "procedures", "simulation", "and", "mod...
2017
Electrical stimulus artifact cancellation and neural spike detection on large multi-electrode arrays
Many cells in mammals exist in the state of quiescence , which is characterized by reversible exit from the cell cycle . Quiescent cells are widely reported to exhibit reduced size , nucleotide synthesis , and metabolic activity . Much lower glycolytic rates have been reported in quiescent compared with proliferating l...
Many cells in the human body are in a reversible state of quiescence , where they have exited the cell cycle but retain the capacity to re-enter it and divide again . Previous experiments in lymphocytes had suggested that quiescent cells reduce their glucose uptake and metabolic rate . In our studies , we have investig...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/cell", "growth", "and", "division" ]
2010
Quiescent Fibroblasts Exhibit High Metabolic Activity
The physiological functions of epidermal cells are largely determined by their diverse morphologies . Most flowering plants have special conical-shaped petal epidermal cells that are thought to influence light capture and reflectance , and provide pollinator grips , but the molecular mechanisms controlling conical cell...
How cells achieve their final shapes is a fundamental question in biology . Most flowering plants have special conical-shaped petal epidermal cells that are thought to attract pollinators , but the molecular and genetic mechanisms that control conical cell shape remain unknown . In this study , we developed a live-conf...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "skin", "plant", "anatomy", "trichomes", "medicine", "and", "health", "sciences", "cell", "motility", "actin", "filaments", "integumentary", "system", "microtubules", "brassica", "research", "design", "plant", "science", "model", "organisms", "experimental", "organism",...
2017
Spatio-temporal orientation of microtubules controls conical cell shape in Arabidopsis thaliana petals
Discovery and characterization of functional RNA structures remains challenging due to deficiencies in de novo secondary structure modeling . Here we describe a dynamic programming approach for model-free sequence comparison that incorporates high-throughput chemical probing data . Based on SHAPE probing data alone , r...
Despite the clear functional importance of structure in RNA molecules , it remains very difficult to correctly identify and annotate similar RNA structures because their sequences are often poorly conserved even for RNAs that form very similar higher-order structures . A solution is to use a metric that identifies stru...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Model-Free RNA Sequence and Structure Alignment Informed by SHAPE Probing Reveals a Conserved Alternate Secondary Structure for 16S rRNA
Information on global human movement patterns is central to spatial epidemiological models used to predict the behavior of influenza and other infectious diseases . Yet it remains difficult to test which modes of dispersal drive pathogen spread at various geographic scales using standard epidemiological data alone . Ev...
What explains the geographic dispersal of emerging pathogens ? Reconstructions of evolutionary history from pathogen gene sequences offer qualitative descriptions of spatial spread , but current approaches are poorly equipped to formally test and quantify the contribution of different potential explanatory factors , su...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "molecular", "epidemiology", "infectious", "disease", "epidemiology", "virology", "epidemiology", "population", "biology", "microbiology", "biology", "spatial", "epidemiology", "computational", "biology", "viral", "evolution" ]
2014
Unifying Viral Genetics and Human Transportation Data to Predict the Global Transmission Dynamics of Human Influenza H3N2
The osteoblast-lineage consists of cells at various stages of maturation that are essential for skeletal development , growth , and maintenance . Over the past decade , many of the signaling cascades that regulate this lineage have been elucidated; however , little is known of the networks that coordinate , modulate , ...
The osteoblast-lineage consists of a range of cells from osteogenic precursors that mature into bone-forming osteoblasts to osteocytes that are entombed in bone . Each cell in the lineage serves a number of distinct and critical roles in the growth and maintenance of the skeleton , as well as many extra-skeletal functi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "models", "genome-wide", "association", "studies", "systems", "biology", "model", "organisms", "gene", "expression", "genetics", "mouse", "biology", "gene", "networks", "genetics", "and", "genomics" ]
2012
Systems Genetic Analysis of Osteoblast-Lineage Cells
Several medically important mosquito-borne flaviviruses have been detected in Argentina in recent years: Dengue ( DENV ) , St . Louis encephalitis ( SLEV ) , West Nile ( WNV ) and Yellow Fever ( YFV ) viruses . Evidence of Bussuquara virus ( BSQV ) and Ilheus virus ( ILHV ) activity were found , but they have not been ...
Flaviviruses are responsible for a growing disease burden in Argentina and other countries in the Americas . Non-human primates , such as monkeys , can be important hosts in the natural cycle of several flaviviruses . Yellow Fever virus outbreaks occurred in Argentina during 2007–2009 in areas of Misiones and Corriente...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "dengue", "virus", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "geographical", "locations", "microbiology", "argentina", "vertebrat...
2017
Detection of the mosquito-borne flaviviruses, West Nile, Dengue, Saint Louis Encephalitis, Ilheus, Bussuquara, and Yellow Fever in free-ranging black howlers (Alouatta caraya) of Northeastern Argentina
Clonal hematopoiesis of indeterminate potential ( CHIP ) is a recently identified process where older patients accumulate distinct subclones defined by recurring somatic mutations in hematopoietic stem cells . CHIP’s implications for stem cell transplantation have been harder to identify due to the high degree of mutat...
We investigated the impact of clonal hematopoiesis of indeterminate potential on stem cell transplantation outcomes . Interclonal competition intensity was identified as an important determinant of ultimate hematopoietic trajectory . We also investigated the impact of age-related marrow changes and show that , from a m...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "bone", "marrow", "stem", "cells", "cloning", "surgical", "and", "invasive", "medical", "procedures", "bone", "marrow", "cells", "stem", "cells", "hematopoietic", "stem", "cell", "transplantation", "molecular", "biology", "te...
2019
Clonal hematopoiesis of indeterminate potential and its impact on patient trajectories after stem cell transplantation
Helminth parasites are known to be efficient modulators of their host’s immune system . To guarantee their own survival , they induce alongside the classical Th2 a strong regulatory response with high levels of anti-inflammatory cytokines and elevated plasma levels of IgG4 . This particular antibody was shown in differ...
Lymphatic Filariasis , also known as elephantiasis , infects an estimated 39 million people in 73 tropical and sub-tropical countries . The most severe clinical manifestations of the disease include swelling of the scrotal area and lower limbs ( hydrocele and lymphedema ) . It is well admitted that host immune reactivi...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "blood", "cells", "invertebrates", "medicine", "and", "health", "sciences", "neurochemistry", "immune", "cells", "body", "fluids", "enzyme-linked", "immunoassays", "chemical", "compounds", "immunology", "neuroscience", "organic", "compounds", "animals", "brugia", "granulo...
2017
Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes
Influenza A virus pandemics and emerging anti-viral resistance highlight the urgent need for novel generic pharmacological strategies that reduce both viral replication and lung inflammation . We investigated whether the primary enzymatic source of inflammatory cell ROS ( reactive oxygen species ) , Nox2-containing NAD...
Influenza A virus pandemics are imminent and with emerging anti-viral resistance highlight an ongoing , urgent need for novel generic pharmacological strategies . Ideally these strategies should reduce both viral replication and lung inflammation , irrespective of the infecting strain by modulating the host immune resp...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/viral", "infections", "pharmacology", "respiratory", "medicine/respiratory", "infections", "infectious", "diseases/respiratory", "infections" ]
2011
Inhibition of Nox2 Oxidase Activity Ameliorates Influenza A Virus-Induced Lung Inflammation
The zebrafish is a powerful experimental system for uncovering gene function in vertebrate organisms . Nevertheless , studies in the zebrafish have been limited by the approaches available for eliminating gene function . Here we present simple and efficient methods for inducing , detecting , and recovering mutations at...
Many genes are being discovered solely on the basis of their association with a trait or disease , or their relatedness to other known genes , but nevertheless the precise biological functions of these genes remain mysterious . We need new tools to discover the immediate molecular , cellular , and developmental functio...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "model", "organisms", "genetics", "biology", "genetics", "and", "genomics" ]
2012
Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome
Ticks are serious haematophagus arthropod pests and are only second to mosquitoes as vectors of diseases of humans and animals . The salivary glands of the slower feeding hard ticks such as Haemaphysalis longicornis are a rich source of bioactive molecules and are critical to their biologic success , yet distinct molec...
Ticks are notorious ectoparasites that exclusively feed on a host's blood for a period of 10 days or longer . Upon blood-feeding , an adult female tick gains 100–200 times its body weight compared to its pre-feeding stage . Despite the host's armoury of rejection mechanisms , ticks manage to remain attached until a ful...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/neglected", "tropical", "diseases", "microbiology/cellular", "microbiology", "and", "pathogenesis", "developmental", "biology/developmental", "molecular", "mechanisms", "microbiology/parasitology" ]
2009
The Kunitz-Like Modulatory Protein Haemangin Is Vital for Hard Tick Blood-Feeding Success
Bacteria can acquire new traits through horizontal gene transfer . Inappropriate expression of transferred genes , however , can disrupt the physiology of the host bacteria . To reduce this risk , Escherichia coli expresses the nucleoid-associated protein , H-NS , which preferentially binds to horizontally transferred ...
Horizontal gene transfer among bacteria is the major means of acquiring genetic diversity and has been a central factor in bacterial evolution . The expression of horizontally transferred genes could potentially be optimized to permit the host bacteria to expand their habitat . The results of our study suggest that DNA...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2016
H-NS Facilitates Sequence Diversification of Horizontally Transferred DNAs during Their Integration in Host Chromosomes
Household contacts of multibacillary leprosy patients ( HCMB ) constitute the group of individuals at the highest risk of developing leprosy . Early diagnosis and treatment of their index cases combined with Bacille Calmette-Guerin ( BCG ) immunization remain important strategies adopted in Brazil to prevent HCMB from ...
Leprosy remains a global public health issue with an annual new case detection of approximately 200 , 000–250 , 000 patients . The current study targets leprosy patient contacts , who constitute the group of individuals at highest risk of developing the disease . Treatment of the index case ( patient ) and BCG vaccinat...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "mycobacterium", "leprae", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "immunology", "tropical", "diseases", "preventive", "medicine", "bacterial", "diseases", "signs", "and", "symptoms", ...
2017
Interruption of persistent exposure to leprosy combined or not with recent BCG vaccination enhances the response to Mycobacterium leprae specific antigens
The number of recombination events per meiosis varies extensively among individuals . This recombination phenotype differs between female and male , and also among individuals of each gender . In this study , we used high-density SNP genotypes of over 2 , 300 individuals and their offspring in two datasets to character...
Meiotic recombination is essential for the formation of human gametes and is a key process that generates genetic diversity . Given its importance , we would expect the number and location of exchanges to be tightly regulated . However , studies show significant gender and inter-individual variation in genome-wide reco...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/complex", "traits", "genetics", "and", "genomics", "genetics", "and", "genomics/chromosome", "biology" ]
2009
Genetic Analysis of Variation in Human Meiotic Recombination
Rabies remains a serious problem in China with three epidemics since 1949 and the country in the midst of the third epidemic . Significantly , the control of each outbreak has been followed by a rapid reemergence of the disease . In 2005 , the government implemented a rabies national surveillance program that included ...
Since 1949 , there have been three rabies epidemics in China . The country is currently in the midst of a third epidemic . After the first two epidemics were brought under control , there was a rapid reemergence of the disease . In 2005 , the government implemented a national surveillance program and as part of this wo...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "rabies", "veterinary", "diseases", "infectious", "disease", "epidemiology", "virology", "epidemiology", "zoonotic", "diseases", "neglected", "tropical", "diseases", "spatial", "epidemiology", "biology", "microbiology", "viral", "diseas...
2013
Molecular Phylodynamic Analysis Indicates Lineage Displacement Occurred in Chinese Rabies Epidemics between 1949 to 2010
Rift Valley fever ( RVF ) is a vector-borne viral zoonosis of increasing global importance . RVF virus ( RVFV ) is transmitted either through exposure to infected animals or through bites from different species of infected mosquitoes , mainly of Aedes and Culex genera . These mosquitoes are very sensitive to environmen...
Rift Valley fever ( RVF ) is a zoonotic disease that affects domestic livestock and humans . During inter-epizootic periods , the main infection mechanism is suspected to be through bites by infected mosquitoes , mainly of Aedes and Culex genera . In East Africa , RVF outbreaks are known to be closely associated with h...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "public", "health", "and", "epidemiology", "infectious", "disease", "epidemiology", "spatial", "epidemiology", "geoinformatics", "rift", "valley", "fever", "neglected", "tropical", "diseases", "veterinary", "science", "infectious", "diseases", "geography", "ve...
2012
Combining Hydrology and Mosquito Population Models to Identify the Drivers of Rift Valley Fever Emergence in Semi-Arid Regions of West Africa
Interspecific hybridization can introduce genetic variation that aids in adaptation to new or changing environments . Here , we investigate how hybrid adaptation to temperature and nutrient limitation may alter parental genome representation over time . We evolved Saccharomyces cerevisiae x Saccharomyces uvarum hybrids...
Organisms are facing rapid alterations to their habitat due to climate change , the introduction of invasive species , habitat fragmentation , and other human mediated transformations . How species will survive and adapt to these changes is one of the biggest questions of our time . Hybridization , or the mating of dif...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "heterozygosity", "genome", "evolution", "chemical", "compounds", "phosphates", "salts", "cloning", "alleles", "mutation", "fungi", "model", "organisms", "experimental", "organism", "systems", "molecular", "biology", "techniques", "saccharomyces", "research", "and", "anal...
2019
Temperature preference can bias parental genome retention during hybrid evolution
The coronaviruses ( CoVs ) are enveloped viruses of animals and humans associated mostly with enteric and respiratory diseases , such as the severe acute respiratory syndrome and 10–20% of all common colds . A subset of CoVs uses the cell surface aminopeptidase N ( APN ) , a membrane-bound metalloprotease , as a cell e...
The cell surface aminopeptidase N ( APN ) , a membranebound metalloprotease target for cancer therapy , is a major cell entry receptor for coronaviruses ( CoVs ) , agents that cause important respiratory and enteric diseases . In some CoVs , the virus envelope spike glycoprotein ( S ) mediates attachment of the virus p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "humoral", "immunity", "viral", "transmission", "and", "infection", "immunology", "microbiology", "host-pathogen", "interaction", "viral", "structure", "viral", "attachment", "protein", "structure", "glycoproteins", "immune", "system", "proteins", "viral", "entry", "prote...
2012
Structural Bases of Coronavirus Attachment to Host Aminopeptidase N and Its Inhibition by Neutralizing Antibodies
Ischemic strokes have been implicated as a cause of death in Chagas disease patients . Inflammation has been recognized as a key component in all ischemic processes , including the intravascular events triggered by vessel interruption , brain damage and repair . In this study , we evaluated the association between infl...
Chagas disease is caused by Trypanosoma cruzi ( T . cruzi ) , affects 5 . 7 million people worldwide and causes 12 , 000 deaths annually . In the chronic phase of Chagas disease the main cause of death is due to heart failure ( about 80% ) , but cerebral vascular accident or stroke ( about 10% ) contributes to death me...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "inflammatory", "diseases", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "physiology", "cytokines", "gene", "regulation", "regulatory", "proteins", "immunology", "tropical", "diseases", "messenger", "rna", "dna-binding", "proteins", "...
2016
Inflammation Enhances the Risks of Stroke and Death in Chronic Chagas Disease Patients
Chikungunya virus ( CHIKV ) is a re-emerging mosquito-borne virus which causes epidemics of fever , severe joint pain and rash . Between 2005 and 2010 , the East/Central/South African ( ECSA ) genotype was responsible for global explosive outbreaks across India , the Indian Ocean and Southeast Asia . From late 2013 , A...
Chikungunya virus ( CHIKV ) has caused large epidemics of fever , rash , and joint pain around the world in recent years . Three different CHIKV genotypes exist . Infection with one genotype is likely to lead to immune protection ( or cross-protection ) against future infections with a different genotype . However , li...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "blood", "serum", "medicine", "and", "health", "sciences", "immune", "physiology", "enzyme-linked", "immunoassays", "pathology", "and", "laboratory", "medicine", "togaviruses", "chikungunya", "infection", "body", "fluids", "pathogens", "immunology", "tropical", "diseases"...
2016
Antigenic Variation of East/Central/South African and Asian Chikungunya Virus Genotypes in Neutralization by Immune Sera
Mycobacterium tuberculosis ( Mtb ) relies on a specialized set of metabolic pathways to support growth in macrophages . By conducting an extensive , unbiased chemical screen to identify small molecules that inhibit Mtb metabolism within macrophages , we identified a significant number of novel compounds that limit Mtb ...
Human beings are the sole ecological niche for M . tuberculosis ( Mtb ) , and it is estimated that 1 . 8 billion people are currently infected with Mtb . An important aspect of this infection is Mtb’s ability to maintain infection by replicating within macrophages . Within macrophages , Mtb exploits a specialized set o...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Novel Inhibitors of Cholesterol Degradation in Mycobacterium tuberculosis Reveal How the Bacterium’s Metabolism Is Constrained by the Intracellular Environment
Several highly conserved genes play a role in anterior neural plate patterning of vertebrates and in head and brain patterning of insects . However , head involution in Drosophila has impeded a systematic identification of genes required for insect head formation . Therefore , we use the red flour beetle Tribolium cast...
All bilaterian animals evolved from one common ancestor . Previous gene function analyses have revealed that several genes play a role in the patterning of anterior regions in all bilaterian animals , suggesting similar mechanisms underlying anterior nervous system formation in humans and the patterning of the insect h...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "functional", "genomics", "organism", "development", "evolutionary", "biology", "biology", "genomics", "comparative", "genomics", "pattern", "formation", "genetics", "and", "genomics", "evolutionary", "developmental", "biology" ]
2011
Candidate Gene Screen in the Red Flour Beetle Tribolium Reveals Six3 as Ancient Regulator of Anterior Median Head and Central Complex Development
Dengue is the most prevalent mosquito-borne viral disease of humans and is caused by the four serotypes of dengue virus . To estimate the incidence of dengue and other arboviruses , we analyzed the baseline and first year follow-up of a prospective school-based cohort study and their families in three cities in the sta...
Dengue is a major public health problem worldwide . The burden of dengue in Latin America is been increasing in the last years and Mexico is one of the countries with the highest burden in this region . To better understand the transmission of dengue in Mexico we established a school-based prospective cohort study of d...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "dengue", "virus", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "respiratory", "infections", "chikungunya", "infection", "pathogens", "tropical", "diseases", "microbiology", "geographical", "locations", "pulmonology", "viruses", "...
2018
Epidemiology of dengue and other arboviruses in a cohort of school children and their families in Yucatan, Mexico: Baseline and first year follow-up
Human herpesviruses are important causes of potentially severe chronic infections for which T cells are believed to be necessary for control . In order to examine the role of virus-specific CD8 T cells against Varicella Zoster Virus ( VZV ) , we generated a comprehensive panel of potential epitopes predicted in silico ...
Human herpesviruses can cause a wide range of serious infections . They are extremely common and individuals remain latently infected lifelong , with reactivations often causing recurrent or severe disease . T-cells are important in controlling herpesvirus infections and preventing their reactivation , so vaccines that...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "chickenpox", "medicine", "and", "health", "sciences", "immune", "cells", "herpes", "simplex", "immunology", "epstein-barr", "virus", "infectious", "mononucleosis", "sexually", "transmitted", "diseases", "infectious", "diseases", "white", "blood", "cel...
2014
Broadly Reactive Human CD8 T Cells that Recognize an Epitope Conserved between VZV, HSV and EBV
The pathogen Campylobacter jejuni is the principal cause of bacterial food-borne infections . The mechanism ( s ) that contribute to bacterial survival and disease are still poorly understood . In other bacterial species , type VI secretion systems ( T6SS ) are increasingly recognized to contribute to bacterial pathoge...
Bacteria contain a number of secretion systems to export macromolecules to the environment . The bacterial type VI secretion system ( T6SS ) forms a needle-like structure that delivers toxic effector molecules to neighboring eukaryotic and/or prokaryotic cells . Here we report that the important human pathogen Campylob...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "bacterial", "diseases", "infectious", "diseases", "gram", "negative", "host-pathogen", "interaction", "biology", "microbiology", "pathogenesis", "campylobacter", "bacterial", "pathogens" ]
2013
Identification of a Functional Type VI Secretion System in Campylobacter jejuni Conferring Capsule Polysaccharide Sensitive Cytotoxicity
The development of systemic approaches in biology has put emphasis on identifying genetic modules whose behavior can be modeled accurately so as to gain insight into their structure and function . However , most gene circuits in a cell are under control of external signals and thus , quantitative agreement between expe...
Circadian clocks keep time of day in many living organisms , allowing them to anticipate environmental changes induced by day/night alternation . They consist of networks of genes and proteins interacting so as to generate biochemical oscillations with a period close to 24 hours . Circadian clocks synchronize to the da...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/signaling", "networks", "cell", "biology/plant", "cell", "biology", "molecular", "biology", "computational", "biology/systems", "biology" ]
2010
Robustness of Circadian Clocks to Daylight Fluctuations: Hints from the Picoeucaryote Ostreococcus tauri
A central question in genomic imprinting is how a specific sequence is recognized as the target for epigenetic marking . In both mammals and plants , imprinted genes are often associated with tandem repeats and transposon-related sequences , but the role of these elements in epigenetic gene silencing remains elusive . ...
Genomic imprinting , mono-allelic gene expression depending on the parent-of-origin , is an epigenetic process known in mammals and flowering plants . A central question in genomic imprinting is how a specific sequence is recognized as the target for epigenetic marking . In both mammals and plants , imprinted genes are...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/epigenetics", "plant", "biology/plant", "genetics", "and", "gene", "expression" ]
2008
Evolution and Control of Imprinted FWA Genes in the Genus Arabidopsis
Leptospirosis is the most widespread zoonosis . Chronic human infection and asymptomatic colonization have been reported . However , renal involvement in those with leptospira chronic exposure remains undetermined . In 2007 , a multistage sampling survey for chronic kidney disease ( CKD ) was conducted in a southern co...
Chronic kidney disease ( CKD ) has a high and increasing worldwide prevalence . Leptospirosis , an important re-emerging infectious disease caused by the pathogenic spirochete Leptospira , is the most widespread zoonosis throughout the world , particularly in tropical and subtropical regions . Chronic human infection a...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Overlooked Risk for Chronic Kidney Disease after Leptospiral Infection: A Population-Based Survey and Epidemiological Cohort Evidence
Localization of objects and events in the environment is critical for survival , as many perceptual and motor tasks rely on estimation of spatial location . Therefore , it seems reasonable to assume that spatial localizations should generally be accurate . Curiously , some previous studies have reported biases in visua...
Almost all daily tasks performed by humans require localizing objects and events . Since spatial localization is critical for survival , it is expected that the brain performs this task accurately . We tested the accuracy of localizing simple sounds and sights in hundreds of human observers . We found that , on average...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Biases in Visual, Auditory, and Audiovisual Perception of Space
The sensory-motor neuron synapse of Aplysia is an excellent model system for investigating the biochemical changes underlying memory formation . In this system , training that is separated by rest periods ( spaced training ) leads to persistent changes in synaptic strength that depend on biochemical pathways that are d...
Memories are among an individual's most cherished possessions . One factor that has been shown to exert a powerful influence on memory formation is the pattern of training . Learning trials distributed over time have been shown to consistently produce longer lasting memories than trials distributed over short intervals...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "models", "molecular", "neuroscience", "behavioral", "neuroscience", "model", "organisms", "cellular", "neuroscience", "computational", "neuroscience", "biology", "computational", "biology", "signaling", "networks", "neuroscience", "learning", "and", "memory" ]
2011
The Rates of Protein Synthesis and Degradation Account for the Differential Response of Neurons to Spaced and Massed Training Protocols
Bacterial chromosomes are organized into polycistronic cotranscribed operons , but the evolutionary pressures maintaining them are unclear . We hypothesized that operons alter gene expression noise characteristics , resulting in selection for or against maintaining operons depending on network architecture . Mathematic...
In some species , most notably bacteria , chromosomal genes are arranged into clusters called operons . In operons , the process of transcription is physically coupled: a single pass of the RNA polymerase enzyme reading that region of the chromosome simultaneously produces messenger RNA encoding multiple proteins . So ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "physics", "biophysics", "microbial", "physiology", "biophysics", "theory", "biology", "evolutionary", "biology", "genomic", "evolution", "microbiology" ]
2012
Interplay of Gene Expression Noise and Ultrasensitive Dynamics Affects Bacterial Operon Organization
Both constitutive secretion and Ca2+-regulated exocytosis require the assembly of the soluble N-ethylmaleimide–sensitive factor attachment protein receptor ( SNARE ) complexes . At present , little is known about how the SNARE complexes mediating these two distinct pathways differ in structure . Using the Drosophila ne...
Most living cells constantly renew their membrane compositions and frequently communicate with neighboring cells by delivering cargo molecules from small vesicles . A key step in cargo delivery requires the fusion of the vesicle membrane with the target membrane mediated by SNARE proteins . In most cellular compartment...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "cell", "biology", "neuroscience", "drosophila" ]
2007
Modification of a Hydrophobic Layer by a Point Mutation in Syntaxin 1A Regulates the Rate of Synaptic Vesicle Fusion
Essential tremor ( ET ) , a movement disorder characterised by an uncontrollable shaking of the affected body part , is often professed to be the most common movement disorder , affecting up to one percent of adults over 40 years of age . The precise cause of ET is unknown , however pathological oscillations of a netwo...
Essential tremor ( ET ) is acknowledged to be the most common movement disorder affecting 1% of the population . Although the underlying mechanisms remain elusive , the thalamus , cortex and cerebellum are implicated in the underlying pathology . More recently , it has been shown that ET can be successfully treated by ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion", "Conclusions" ]
[ "medicine", "and", "health", "sciences", "myoclonus", "pathology", "and", "laboratory", "medicine", "neural", "networks", "brain", "brain", "electrophysiology", "electrophysiology", "neuroscience", "deep-brain", "stimulation", "signs", "and", "symptoms", "brain", "mapping...
2017
A Network Model of Local Field Potential Activity in Essential Tremor and the Impact of Deep Brain Stimulation
Enterocytozoon bieneusi is the most common microsporidian associated with human disease , particularly in the immunocompromised population . In the setting of HIV infection , it is associated with diarrhea and wasting syndrome . Like all microsporidia , E . bieneusi is an obligate , intracellular parasite , but unlike ...
Enterocytozoon bieneusi is a clinically significant pathogen associated with human microsporidiosis , particularly in immunocompromised individuals . E . bieneusi is widespread in mammals , and there is no effective commercial treatment for infection . The pathogen cannot be readily cultivated , and animal models are l...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[ "genetics", "and", "genomics/genomics", "molecular", "biology", "infectious", "diseases/gastrointestinal", "infections" ]
2009
Genomic Survey of the Non-Cultivatable Opportunistic Human Pathogen, Enterocytozoon bieneusi
Non-tuberculous mycobacteria ( NTM ) are different from Mycobacterium tuberculosis ( MTB ) both in their ubiquitous environmental distribution and in their reduced capacity to cause disease . While often neglected in favour of other infectious diseases , NTM may interfere with important aspects of TB control and manage...
Non-tuberculous mycobacteria ( NTM ) are a ubiquitous group of mycobacteria found in the environment . They are opportunistic pathogens causing human disease , especially in immunocompromised individuals . Differentiation between NTM infection and tuberculosis ( TB ) can be difficult . Data on incidence of NTM in TB en...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "bacterial", "diseases", "infectious", "diseases", "medicine", "and", "health", "sciences", "tuberculosis", "biology", "and", "life", "sciences", "immunology", "microbiology", "tropical", "diseases", "immune", "response" ]
2014
Effect of Non-tuberculous Mycobacteria on Host Biomarkers Potentially Relevant for Tuberculosis Management
The peptidoglycan ( PG ) sacculus is composed of long glycan strands cross-linked together by short peptides forming a covalently closed meshwork that protects the bacterial cell from osmotic lysis and specifies its shape . PG hydrolases play essential roles in remodeling this three-dimensional network during growth an...
Bacterial growth and division require the synthesis and remodeling of the cell wall exoskeleton . To prevent lethal breaches in this protective layer , peptidoglycan ( PG ) hydrolases that remodel the cell wall must be carefully regulated but the mechanisms underlying this control remain poorly understood . The noncano...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "walls", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "molecular", "probe", "techniques", "enzymes", "pathogens", "cell", "cycle", "and", "cell", "division", "cell", "processes", "bacillus", "enzymology", "immunoblotti...
2019
SweC and SweD are essential co-factors of the FtsEX-CwlO cell wall hydrolase complex in Bacillus subtilis
Extended periods of waking result in physiological impairments in humans , rats , and flies . Sleep homeostasis , the increase in sleep observed following sleep loss , is believed to counter the negative effects of prolonged waking by restoring vital biological processes that are degraded during sleep deprivation . Sle...
It is well established in humans that sleep deficits lead to adverse outcomes , including cognitive impairments and an increased risk for obesity . Given the relationship between sleep and lipid stores , we hypothesized that metabolic pathways play a role in sleep regulation and contribute to deficits induced by sleep ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/behavioral", "neuroscience", "neuroscience/cognitive", "neuroscience", "physiology/integrative", "physiology", "physiology/neural", "homeostasis" ]
2010
The Perilipin Homologue, Lipid Storage Droplet 2, Regulates Sleep Homeostasis and Prevents Learning Impairments Following Sleep Loss
CD4+/CD8+ double positive ( DP ) T cells have been described in healthy individuals as well as in patients with autoimmune and chronic infectious diseases . In chronic viral infections , this cell subset has effector memory phenotype and displays antigen specificity . No previous studies of double positive T cells in p...
Chagas disease , produced by the blood parasite Trypanosoma cruzi , is considered a public health problem in Central and South America . Non sterile immunity can be achieved after acute infection . Parasite persistence can induce tissue damage in nearly 20% to 30% of chronically infected individuals . Indeed , chagasic...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "chagas", "disease", "immune", "cells", "t", "cells", "neglected", "tropical", "diseases", "immunology", "biology" ]
2011
Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients
Genotype-to-phenotype maps exhibit complexity . This genetic complexity is mentioned frequently in the literature , but a consistent and quantitative definition is lacking . Here , we derive such a definition and investigate its consequences for model genetic systems . The definition equates genetic complexity with a s...
‘Genetic complexity’ is an often-discussed property of genotype-to-phenotype maps , but the term is used vaguely and inconsistently in the literature . We derived a definition of genetic complexity that assigns to every genotype-to-phenotype map a unique quantitative measure of its genetic complexity . This definition ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "systems", "biology", "regulatory", "networks", "synthetic", "biology", "biology", "computational", "biology" ]
2012
Quantifying and Analyzing the Network Basis of Genetic Complexity
We recently discovered an inherited cancer syndrome caused by BRCA1-Associated Protein 1 ( BAP1 ) germline mutations , with high incidence of mesothelioma , uveal melanoma and other cancers and very high penetrance by age 55 . To identify families with the BAP1 cancer syndrome , we screened patients with family histori...
Germline BAP1 mutations cause a cancer syndrome characterized by high incidence of mesothelioma ( MM ) , uveal melanoma and other cancers , and by very high penetrance , as all individuals carrying BAP1 mutations developed at least one , and usually several , malignancies throughout their lives . Through screening MM p...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Combined Genetic and Genealogic Studies Uncover a Large BAP1 Cancer Syndrome Kindred Tracing Back Nine Generations to a Common Ancestor from the 1700s
P . vivax infection during pregnancy has been associated with poor outcomes such as anemia , low birth weight and congenital malaria , thus representing an important global health problem . However , no vaccine is currently available for its prevention . Vir genes were the first putative virulent factors associated wit...
Naturally-acquired antibody responses to novel recombinant proteins and synthetic peptides based on sequences from P . vivax VIR antigens were evaluated in women from five distinct geographical regions endemic for malaria , during and after pregnancy . Levels of IgG to VIR antigens were indicative of cumulative malaria...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "body", "weight", "parasite", "groups", "medicine", "and", "health", "sciences", "immune", "physiology", "immune", "cells", "maternal", "health", "plasmodium", "obstetrics", "and", "gynecology", "immunology", "tropical", "diseases", "parasitic", "dis...
2016
Plasmodium vivax VIR Proteins Are Targets of Naturally-Acquired Antibody and T Cell Immune Responses to Malaria in Pregnant Women
Salmonella enterica serovar Infantis is one of the prevalent Salmonella serovars worldwide . Different emergent clones of S . Infantis were shown to acquire the pESI virulence-resistance megaplasmid affecting its ecology and pathogenicity . Here , we studied two previously uncharacterized pESI-encoded chaperone-usher f...
Salmonella enterica serovar Infantis is one of the prevalent serovars worldwide and is often associated with human gastroenteritis and asymptomatic persistence in poultry . Different emergent S . Infantis populations were shown to acquire a large virulence-resistance plasmid , named pESI . Here we imaged and investigat...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "microbiology", "pili", "and", "fimbriae", "animal", "models", "bacterial", "diseases", "mutation", "regulator", "genes", "model", "organisms", "e...
2017
The plasmid-encoded Ipf and Klf fimbriae display different expression and varying roles in the virulence of Salmonella enterica serovar Infantis in mouse vs. avian hosts
Control and elimination of zoonotic diseases requires robust information about their effect on both human and livestock health in order to enable policy formulation and the allocation of resources . This study aimed to evaluate the cost-effectiveness of controlling Taenia solium taeniasis/cysticercosis in both humans a...
A study was conducted in northern Lao PDR to ascertain the cost-effectiveness of controlling Taenia solium ( T . solium taeniasis/cysticercosis ) using five approaches namely: i ) T . solium taeniasis/cysticercosis alone in the human population ( the base comparator ) , ii ) T . solium taeniasis/cysticercosis and soil ...
[ "Abstract", "Introduction", "Methodology", "Results", "Discussion", "Conclusion" ]
[ "livestock", "animal", "diseases", "medicine", "and", "health", "sciences", "cost-effectiveness", "analysis", "economic", "analysis", "social", "sciences", "vertebrates", "animals", "mammals", "health", "care", "age", "groups", "zoology", "veterinary", "science", "epile...
2018
Improved methods to capture the total societal benefits of zoonotic disease control: Demonstrating the cost-effectiveness of an integrated control programme for Taenia solium, soil transmitted helminths and classical swine fever in northern Lao PDR
We present a framework for designing cheap control architectures of embodied agents . Our derivation is guided by the classical problem of universal approximation , whereby we explore the possibility of exploiting the agent’s embodiment for a new and more efficient universal approximation of behaviors generated by sens...
Given a body and an environment , what is the brain complexity needed in order to generate a desired set of behaviors ? The general understanding is that the physical properties of the body and the environment correlate with the required brain complexity . More precisely , it has been pointed that naturally evolved int...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
A Theory of Cheap Control in Embodied Systems
The effect of calorie restriction ( CR ) on life span extension , demonstrated in organisms ranging from yeast to mice , may involve the down-regulation of pathways , including Tor , Akt , and Ras . Here , we present data suggesting that yeast Tor1 and Sch9 ( a homolog of the mammalian kinases Akt and S6K ) is a centra...
Studies using model organisms have pointed to the existence of evolutionarily conserved genes and signaling pathways that regulates life span . Changes in the activity of these genes/pathways have also been implicated in mediating the beneficial effect of calorie restriction , a well-recognized intervention that extend...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/cellular", "death", "and", "stress", "responses", "cell", "biology/gene", "expression", "cell", "biology/cell", "signaling" ]
2009
Tor1/Sch9-Regulated Carbon Source Substitution Is as Effective as Calorie Restriction in Life Span Extension
Adenosine-to-inosine RNA editing diversifies the transcriptome and promotes functional diversity , particularly in the brain . A plethora of editing sites has been recently identified; however , how they are selected and regulated and which are functionally important are largely unknown . Here we show the cis-regulatio...
Many important modifications are made to RNA to fine-tune genomic information . One type , Adenosine-to-Inosine ( A-to-I ) RNA editing , changes certain adenosines to inosines and is essential for the neurological well-being of many animals . Although RNA editing occurs at thousands of sites across the genomes of vario...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "sequencing", "techniques", "invertebrates", "gene", "regulation", "animals", "animal", "models", "drosophila", "melanogaster", "model", "organisms", "micrornas", "non-coding", "rna", "experimental", "organism", "systems", "sequence", "motif", "analysis", "molecular", "bi...
2017
Evolutionary analysis reveals regulatory and functional landscape of coding and non-coding RNA editing
The ability of plants to provide a plastic response to environmental cues relies on the connectivity between signaling pathways . DELLA proteins act as hubs that relay environmental information to the multiple transcriptional circuits that control growth and development through physical interaction with transcription f...
Plants respond to environmental cues by modulating transcriptional circuits . One mechanism for such modulation involves DELLA proteins . They are promiscuous interactors of transcription factors and , in most cases , this interaction impairs the recognition of the DNA target sequences . Here we show that DELLA protein...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Genome Wide Binding Site Analysis Reveals Transcriptional Coactivation of Cytokinin-Responsive Genes by DELLA Proteins
Dengue viral infection is a global health threat without vaccine or specific treatment . The clinical outcome varies from asymptomatic , mild dengue fever ( DF ) to severe dengue hemorrhagic fever ( DHF ) . While adaptive immune responses were found to be detrimental in the dengue pathogenesis , the roles of earlier in...
Almost half of the world population is at risk of dengue viral infection . The disease severity varies from mild to a deadly form-which is caused mainly by host overt immune reaction . Earlier studies focused on the disease-causing roles of adaptive immune cells - cells that are highly specific but require time and sig...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "innate", "immune", "system", "medicine", "and", "health", "sciences", "emerging", "viral", "diseases", "immunity", "to", "infections", "immunology", "tropical", "diseases", "microbiology", "emerging", "infectious", "diseases", "neglected", "tropical", "diseases", "infe...
2014
Invariant NKT Cell Response to Dengue Virus Infection in Human
Babesia , a tick-borne genus of intraerythrocytic parasites , is understudied in humans outside of established high-endemic areas . There is a paucity of data on Babesia in Africa , despite evidence that it is regionally present . A pilot study suggested that Babesia was present in a rural district of Tanzania . A cros...
Babesia , a family of tick-borne parasites , causes babesiosis , a disease that is very similar to malaria . Babesia species are globally ubiquitous yet understudied in humans outside of a few areas of the world , most notably in the United States . There is very little , published information on Babesia in humans in A...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "parasite", "groups", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "body", "fluids", "ixodes", "plasmodium", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "animals", "parasitology", "babesiosis", ...
2019
The Babesia observational antibody (BAOBAB) study: A cross-sectional evaluation of Babesia in two communities in Kilosa district, Tanzania
Tsetse flies ( Glossina spp . ) are vectors of parasitic trypanosomes , which cause human ( HAT ) and animal African trypanosomiasis ( AAT ) in sub-Saharan Africa . In Uganda , Glossina fuscipes fuscipes ( Gff ) is the main vector of HAT , where it transmits Gambiense disease in the northwest and Rhodesiense disease in...
We investigated the association of symbiotic Spiroplasma with the tsetse fly host Glossina fuscipes fuscipes ( Gff ) to assess if Spiroplasma infections are correlated with Gff genetic background , geography , or season and its interaction with trypanosome parasites . We analyzed distribution and prevalence of Spiropla...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "population", "genetics", "geographical", "locations", "uganda", "parasitic", "diseases", "parasitic", "protozoans", "wolbachia", "protozoans", "population", "biology", "bacteria", "haplogroups", "africa", "spiroplasma", "infectious"...
2019
Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda
Monoubiquitination of histone H2B lysine 123 regulates methylation of histone H3 lysine 4 ( H3K4 ) and 79 ( H3K79 ) and the lack of H2B ubiquitination in Saccharomyces cerevisiae coincides with metacaspase-dependent apoptosis . Here , we discovered that loss of H3K4 methylation due to depletion of the methyltransferase...
Covalent histone modifications alter chromatin structure and DNA accessibility , which is playing important roles in a wide range of DNA-based processes , such as transcription regulation and DNA repair , but also cell division and apoptosis . Apoptosis is the most common form of programmed cell death and plays importa...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "death", "cellular", "structures", "gene", "expression", "cell", "nucleus", "biology", "molecular", "cell", "biology", "histone", "modification" ]
2014
Loss of Histone H3 Methylation at Lysine 4 Triggers Apoptosis in Saccharomyces cerevisiae
A better knowledge of the flow and pressure distribution in realistic microvascular networks is needed for improving our understanding of neurovascular coupling mechanisms and the related measurement techniques . Here , numerical simulations with discrete tracking of red blood cells ( RBCs ) are performed in three real...
The brain consumes approximately 20% of the total oxygen used by the human body . An efficient and robust energy supply is essential for the brain’s functioning . The brain is able to up-regulate its oxygen supply in the proximity of neuronal activation . However , the details of the underlying vascular regulation mech...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "classical", "mechanics", "fluid", "mechanics", "cardiovascular", "anatomy", "oxygen", "capillaries", "arterioles", "blood", "vessels", "flow", "field", "chemistry", "fluid", "dynamics", "blood", "pressure", "...
2017
Depth-dependent flow and pressure characteristics in cortical microvascular networks
Iron is essential for many cellular processes , but can generate highly toxic hydroxyl radicals in the presence of oxygen . Therefore , intracellular iron accumulation must be tightly regulated , by balancing uptake with storage or export . Iron uptake in Leishmania is mediated by the coordinated action of two plasma m...
Leishmaniasis is a human infectious disease that is endemic in many tropical and subtropical areas of the world . It is caused by transmission of the protozoan parasite Leishmania through bites of infected sand flies . The symptoms of leishmaniasis range from disfiguring skin lesions to potentially deadly visceralizing...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "brassica", "microbiology", "parasitic", "diseases", "protozoan", "life", "cycles", "parasitic", "protozoans", "membrane", "proteins", "developmental", "biology", "model", "organisms", "protozoans", "leishmania", "experimental", "o...
2018
A MFS-like plasma membrane transporter required for Leishmania virulence protects the parasites from iron toxicity
The stringent response is initiated by rapid ( p ) ppGpp synthesis , which leads to a profound reprogramming of gene expression in most bacteria . The stringent phenotype seems to be species specific and may be mediated by fundamentally different molecular mechanisms . In Staphylococcus aureus , ( p ) ppGpp synthesis u...
The stringent response is a bacterial response to a multitude of different environmental stress conditions which is characterized by the synthesis of the messenger molecules ( p ) ppGpp . There is now growing evidence that these molecules also play a key role for pathogens to switch between specific phenotypic states w...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacteriology", "bacterial", "physiology", "staphylococci", "biology", "microbiology", "host-pathogen", "interaction", "bacterial", "pathogens", "pathogenesis" ]
2012
The Stringent Response of Staphylococcus aureus and Its Impact on Survival after Phagocytosis through the Induction of Intracellular PSMs Expression
In low elevation arid regions throughout the southern United States , Borrelia turicatae is the principal agent of tick-borne relapsing fever . However , endemic foci and the vertebrate hosts involved in the ecology of B . turicatae remain undefined . Experimental infection studies suggest that small and medium sized m...
In arid regions of the southern United States and Mexico , tick-borne relapsing fever is primarily caused by Borrelia turicatae . The tick vector , Ornithodoros turicata , feeds indiscriminately on a variety of vertebrates; however , it is unclear which animals are competent hosts for B . turicatae . This study evaluat...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "united", "states", "borrelia", "infection", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "geographical", "locations", "microbiology", "vertebrates", "animals", "mammals", "north", "america", "bacterial", "diseases", ...
2018
Seroprevalence for the tick-borne relapsing fever spirochete Borrelia turicatae among small and medium sized mammals of Texas
Highly pathogenic avian influenza viruses of the H5N1 subtype continue to threaten agriculture and human health . Here , we use biochemistry and x-ray crystallography to reveal how amino-acid variations in the hemagglutinin ( HA ) protein contribute to the pathogenicity of H5N1 influenza virus in chickens . HA proteins...
To deliver their genomes into host cells during entry , enveloped viruses contain glycoproteins that bind to cellular receptors and cause fusion of viral and cellular membranes . The influenza virus HA protein is the archetypal viral fusion glycoprotein , promoting entry by undergoing irreversible structural changes th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "influenza", "microbiology", "viral", "structure", "membrane", "proteins", "protein", "structure", "infectious", "diseases", "cell", "membrane", "cytochemistry", "proteins", "biology", "pathogenesis", "biochemistry", "virology", "viral", "diseases" ]
2011
Acid Stability of the Hemagglutinin Protein Regulates H5N1 Influenza Virus Pathogenicity
The Pol32 protein is one of the universal subunits of DNA polymerase δ ( Pol δ ) , which is responsible for genome replication in eukaryotic cells . Although the role of Pol32 in DNA repair has been well-characterized , its exact function in genome replication remains obscure as studies in single cell systems have not ...
Cancer etiological studies suggest that the majority of pathological mutations occurred under near normal DNA replication conditions , emphasizing the importance of understanding replication regulation under non-lethal conditions . To gain such a better understanding , we investigated the function of Pol32 , a conserve...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "dna-binding", "proteins", "animals", "invertebrate", "genomics", "animal", "models", "developmental", "biology", "drosophila", "melanogaster", "model", "organisms", "polymerases", "dna", "replication", "experimental", "organism", "systems", "dna", "mammali...
2019
The processivity factor Pol32 mediates nuclear localization of DNA polymerase delta and prevents chromosomal fragile site formation in Drosophila development
African trypanosomes are digenetic parasites that undergo part of their developmental cycle in mammals and part in tsetse flies . We established a novel technique to monitor the population dynamics of Trypanosoma brucei throughout its life cycle while minimising the confounding factors of strain differences or variatio...
African trypanosomes cause human sleeping sickness and Nagana in domestic animals . These parasites undergo part of their life cycle in mammalian hosts and part in their insect host , the tsetse fly , and are transmitted when the fly takes a blood meal . Within the fly , successful transmission involves two steps: colo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/protozoal", "infections", "microbiology/parasitology", "developmental", "biology/microbial", "growth", "and", "development" ]
2010
Bottlenecks and the Maintenance of Minor Genotypes during the Life Cycle of Trypanosoma brucei
The differentiation of self-renewing progenitor cells requires not only the regulation of lineage- and developmental stage–specific genes but also the coordinated adaptation of housekeeping functions from a metabolically active , proliferative state toward quiescence . How metabolic and cell-cycle states are coordinate...
The human body is made from billions of cells comprizing many specialized cell types . All of these cells ultimately come from a single fertilized oocyte in a process that has two key features: proliferation , which expands cell numbers , and differentiation , which diversifies cell types . Here , we have examined the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2019
Feedforward regulation of Myc coordinates lineage-specific with housekeeping gene expression during B cell progenitor cell differentiation
NMDA ( N-methyl-D-aspartate ) receptors and calcium can exert multiple and very divergent effects within neuronal cells , thereby impacting opposing occurrences such as synaptic plasticity and neuronal degeneration . The neuronal Ca2+ sensor Caldendrin is a postsynaptic density component with high similarity to calmodu...
Long-lasting changes in communication between nerve cells require the regulation of gene expression . The influx of calcium ions into the cell , particularly through membrane protein called NMDA receptors , plays a crucial role in this process by determining the type of gene expression induced . NMDA receptors can exer...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "neuroscience", "cell", "biology" ]
2008
Caldendrin–Jacob: A Protein Liaison That Couples NMDA Receptor Signalling to the Nucleus
In phylogenetically diverse animals , including the basally diverging cnidarians , “determinants” localised within the egg are responsible for directing development of the embryonic body plan . Many such determinants are known to regulate the Wnt signalling pathway , leading to regionalised stabilisation of the transcr...
How do different animal body parts form in the correct arrangement during development ? Often , the explanation is provided by “determinant” molecules , prepositioned in the egg cell before it is fertilised . These determinant molecules initiate spatially localized programmes of gene expression , causing the various bo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "ascidians", "(sea", "squirts)", "developmental", "biology", "cell", "biology", "eukaryotes", "evolutionary", "biology" ]
2007
Two Oppositely Localised Frizzled RNAs as Axis Determinants in a Cnidarian Embryo
Polyclonal Epstein-Barr virus ( EBV ) -infected B cell line ( lymphoblastoid cell lines; LCL ) -stimulated T-cell preparations have been successfully used to treat EBV-positive post-transplant lymphoproliferative disorders ( PTLD ) in transplant recipients , but function and specificity of the CD4+ component are still ...
The γ-herpesvirus Epstein-Barr virus ( EBV ) is associated with several human malignancies , including post-transplant lymphoproliferative disorders ( PTLD ) in immunocompromised patients . The successful treatment of EBV-positive PTLD by the infusion of EBV-specific T-cell lines has provided an important proof of prin...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology", "infectious", "diseases", "medicine", "and", "health", "sciences", "clinical", "immunology", "immunity", "virology", "cancer", "treatment", "biology", "and", "life", "sciences", "immunology", "microbiology", "viral", "diseases", "immunotherapy" ]
2014
Virus and Autoantigen-Specific CD4+ T Cells Are Key Effectors in a SCID Mouse Model of EBV-Associated Post-Transplant Lymphoproliferative Disorders
Several experiments indicate that there exists substantial synaptic-depression at the synapses between olfactory receptor neurons ( ORNs ) and neurons within the drosophila antenna lobe ( AL ) . This synaptic-depression may be partly caused by vesicle-depletion , and partly caused by presynaptic-inhibition due to the a...
Understanding the intricacies of sensory processing is a major scientific challenge . In this paper we examine the early stages of the olfactory system of the fruit-fly . Many experiments have revealed a great deal regarding the architecture of this system , including the types of neurons within it , as well as the con...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "computational", "neuroscience", "biology", "neuroscience" ]
2012
Functional Roles for Synaptic-Depression within a Model of the Fly Antennal Lobe
Strongyloidiasis is frequently under diagnosed since many infections remain asymptomatic and conventional diagnostic tests based on parasitological examination are not sufficiently sensitive . Serology is useful but is still only available in reference laboratories . The need for improved diagnostic tests in terms of s...
Strongyloidiasis is a parasitic infection that can occur in any place of the world . It is not easy to diagnose because the conventional tests are not good enough , especially in individuals that do not present any symptoms of the disease . This is of particular importance in immunocompromised patients , because the di...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "public", "health", "and", "epidemiology", "epidemiology", "public", "health" ]
2013
The Laboratory Diagnosis and Follow Up of Strongyloidiasis: A Systematic Review
The hallmark of canonical Wnt signaling is the transcriptional induction of Wnt target genes by the beta-catenin/TCF complex . Several studies have proposed alternative interaction partners for beta-catenin or TCF , but the relevance of potential bifurcations in the distal Wnt pathway remains unclear . Here we study on...
Our manuscript addresses the question of whether either of the canonical transduction components , beta-catenin or TCF , can be bypassed when the Wnt pathway is activated . By using somatic cell genetics in Drosophila cells ( via CRISPR/Cas9 editing ) in combination with RNA-seq and STARR-seq ( Self-transcribing-active...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "luciferase", "enzymes", "enzymology", "animals", "invertebrate", "genomics", "dna", "transcription", "animal", "models", "drosophila", "melanogaster", "model", "organisms", "experimental", "organism", "systems", "sequence", "motif", "analysis", "drosophila...
2017
Probing the canonicity of the Wnt/Wingless signaling pathway
The rising prevalence of arthritogenic alphavirus infections , including chikungunya virus ( CHIKV ) and Ross River virus ( RRV ) , and the lack of antiviral treatments highlight the potential threat of a global alphavirus pandemic . The immune responses underlying alphavirus virulence remain enigmatic . We found that ...
Chikungunya virus ( CHIKV ) and Ross River virus ( RRV ) are arthropod-borne viruses associated with massive epidemics affecting millions of people worldwide , causing widespread distribution of alphaviral-induced arthritis . The rising prevalence of alphavirus infections and , critically , the lack of therapeutic trea...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Role of Pentraxin 3 in Shaping Arthritogenic Alphaviral Disease: From Enhanced Viral Replication to Immunomodulation
Detection of viral nucleic acids plays a critical role in the induction of intracellular host immune defences . However , the temporal recruitment of immune regulators to infecting viral genomes remains poorly defined due to the technical difficulties associated with low genome copy-number detection . Here we utilize 5...
Intrinsic and innate immunity act to restrict the replication of many clinically important viral pathogens . However , the temporal regulation of these two arms of host immunity during virus infection remains poorly defined . A key aspect in the regulation of these intracellular immune defences during herpesvirus infec...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "immunology", "microbiology", "virus", "effects", "on", "host", "gene", "expression", "viral", "genome", "microbial", "genetics", "microbial", "genomics", "viral", "genomics", "infectious", "diseases", "gene", "expression", "vi...
2018
Distinct temporal roles for the promyelocytic leukaemia (PML) protein in the sequential regulation of intracellular host immunity to HSV-1 infection
Cryptococcus neoformans is a haploid environmental organism and the major cause of fungal meningoencephalitis in AIDS patients . Fluconazole ( FLC ) , a triazole , is widely used for the maintenance therapy of cryptococcosis . Heteroresistance to FLC , an adaptive mode of azole resistance , was associated with FLC ther...
Cryptococcus neoformans is an environmental fungus that causes life threatening brain disease , primarily in AIDS patients . The disease is estimated to claim 700 , 000 lives annually world-wide but most heavily in Africa . Fluconazole ( FLC ) , a fungistatic antifungal drug , is commonly used to treat patients for lon...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/fungal", "infections", "microbiology", "molecular", "biology", "microbiology/medical", "microbiology", "infectious", "diseases/antimicrobials", "and", "drug", "resistance" ]
2010
Cryptococcus neoformans Overcomes Stress of Azole Drugs by Formation of Disomy in Specific Multiple Chromosomes
Current efforts to control human soil-transmitted helminths ( STHs ) involve the periodic mass administration of benzimidazole drugs to school aged children and other at- risk groups . Given that high levels of resistance to these drugs have developed in roundworms of livestock , there is a need to monitor drug efficac...
With the implementation of mass drug administration programmes for the control of human soil transmitted helminths there is a need to develop drug sensitivity monitoring tools to detect the emergence of resistance . The present study aimed to use an egg hatch assay to measure benzimidazole sensitivity in human hookworm...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "hookworm", "infection", "neglected", "tropical", "diseases", "hookworm", "parasitic", "diseases" ]
2011
The Effect of Egg Embryonation on Field-Use of a Hookworm Benzimidazole-Sensitivity Egg Hatch Assay in Yunnan Province, People's Republic of China
The aberrant expression of the transmembrane protein EpCAM is associated with tumor progression , affecting different cellular processes such as cell–cell adhesion , migration , proliferation , differentiation , signaling , and invasion . However , the in vivo function of EpCAM still remains elusive due to the lack of ...
EpCAM is a well-established marker for carcinomas of epithelial origin and a potential target for immunotherapy . In vitro analyses have implicated EpCAM in a plethora of different cellular processes , such as adhesion , motility , proliferation , differentiation , and signaling . Strikingly , depending on the context ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "dermatology", "developmental", "biology", "developmental", "biology/morphogenesis", "and", "cell", "biology", "developmental", "biology/embryology" ]
2009
The Epithelial Cell Adhesion Molecule EpCAM Is Required for Epithelial Morphogenesis and Integrity during Zebrafish Epiboly and Skin Development
Although misfolding of normal prion protein ( PrPC ) into abnormal conformers ( PrPSc ) is critical for prion disease pathogenesis our current understanding of the underlying molecular pathophysiology is rudimentary . Exploiting an electrophysiology paradigm , herein we report that at least modestly proteinase K ( PK )...
Misfolding of the normal prion protein ( PrPC ) into disease-associated conformations ( PrPSc ) is the critical initiating step for prion diseases . Similar to other neurodegenerative disorders , progressive failure of brain synapses is considered a primary deleterious event underpinning prion disease evolution . Our c...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "molecular", "probe", "techniques", "nervous", "system", "brain", "electrophysiology", "immunoblotting", "electrophysiology", "neuroscience", "bioassays", "and", "physiological", "analysis", "molecular", "biology", "techniques", "neu...
2018
Prion acute synaptotoxicity is largely driven by protease-resistant PrPSc species
Although rabies represents an important public health threat , it is still a neglected disease in Asia and Africa where it causes tens of thousands of deaths annually despite available human and animal vaccines . In the Central African Republic ( CAR ) , an endemic country for rabies , this disease remains poorly inves...
Rabies is a widespread fatal , but preventable , viral disease transmitted from animals to humans . It has been estimated that tens of thousands of people die of rabies annually , mainly in developing countries where rabies is still a neglected disease . In the Central African Republic ( CAR ) , a poor country located ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "tropical", "diseases", "geographical", "locations", "vertebrates", "microbiology", "dogs", "animals", "mammals", "viruses", "preventive", "medicine", "rabies...
2016
Surveillance of Canine Rabies in the Central African Republic: Impact on Human Health and Molecular Epidemiology
Staphylococcus aureus contains an autoinducing quorum-sensing system encoded within the agr operon that coordinates expression of virulence genes required for invasive infection . Allelic variation within agr has generated four agr specific groups , agr I–IV , each of which secretes a distinct autoinducing peptide pher...
Staphylococcus aureus is a common colonizer of humans but can also cause severe , invasive infection . S . aureus uses a secreted peptide-based communication system , agr , to induce production of virulence factors needed for invasive infection . Allelic variation has generated four agr types , agr I–IV , and each secr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "immunology", "biology", "microbiology" ]
2013
Nox2 Modification of LDL Is Essential for Optimal Apolipoprotein B-mediated Control of agr Type III Staphylococcus aureus Quorum-sensing
In prion diseases , synapse dysfunction , axon retraction and loss of neuronal polarity precede neuronal death . The mechanisms driving such polarization defects , however , remain unclear . Here , we examined the contribution of RhoA-associated coiled-coil containing kinases ( ROCK ) , key players in neuritogenesis , ...
Transmissible Spongiform Encephalopathies ( TSEs ) , commonly named prion diseases , are caused by deposition in the brain of pathogenic prions PrPSc that trigger massive neuronal death . Because of our poor understanding of the mechanisms sustaining prion-induced neurodegeneration , there is to date no effective medic...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation
It is increasingly clear that transcription factors play versatile roles in turning genes “on” or “off” depending on cellular context via the various transcription complexes they form . This poses a major challenge in unraveling combinatorial transcription complex codes . Here we use the powerful genetics of Drosophila...
Different cell types in multi-cellular organisms are determined by the repertoire of genes active in each cell . This repertoire , or transcriptome , is established by the coordinated activity of transcription factors and cofactors that form modular transcription complexes . The modular nature of transcription complexe...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "computational", "biology/molecular", "genetics", "developmental", "biology/developmental", "molecular", "mechanisms", "computational", "biology/genomics", "genetics", "and", "genomics/gene", "expression" ]
2010
Transcriptional Regulation by CHIP/LDB Complexes
An expanded chemical space is essential for improved identification of small molecules for emerging therapeutic targets . However , the identification of targets for novel compounds is biased towards the synthesis of known scaffolds that bind familiar protein families , limiting the exploration of chemical space . To c...
Bioactive compounds often disrupt cellular gene expression in ways that are difficult to predict . While the correlation between a cellular response after treatment with a small molecule and the knockdown of its target protein should be simple to establish , in practice this goal has been difficult to achieve . The mai...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "biotechnology", "medicine", "and", "health", "sciences", "crystal", "structure", "chemical", "compounds", "engineering", "and", "technology", "small", "molecules", "condensed", "matter", "physics", "organic", "compounds", "pharmaceutics", "pharmacology", "crystallography",...
2018
Predicting protein targets for drug-like compounds using transcriptomics