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HIV-1 Viral protein R ( Vpr ) induces a cell cycle arrest at the G2/M phase by activating the ATR DNA damage/stress checkpoint . Recently , we and several other groups showed that Vpr performs this activity by recruiting the DDB1-CUL4A ( VPRBP ) E3 ubiquitin ligase . While recruitment of this E3 ubiquitin ligase comple...
HIV-1 , the causative agent of AIDS , encodes several proteins termed accessory , which play a critical role in viral pathogenesis . One of these accessory proteins , viral protein R ( Vpr ) , has been found to block normal cell division . This impairment of cell division by Vpr is thought to increase viral replication...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/virulence", "factors", "and", "mechanisms", "cell", "biology/cellular", "death", "and", "stress", "responses", "cell", "biology/cell", "signaling", "virology/immunodeficiency", "viruses", "biochemistry/replication", "and", "repair" ]
2010
Formation of Mobile Chromatin-Associated Nuclear Foci Containing HIV-1 Vpr and VPRBP Is Critical for the Induction of G2 Cell Cycle Arrest
In networks of excitatory and inhibitory neurons with mutual synaptic coupling , specific drive to sub-ensembles of cells often leads to gamma-frequency ( 25–100 Hz ) oscillations . When the number of driven cells is too small , however , the synaptic interactions may not be strong or homogeneous enough to support the ...
Gamma-frequency ( 25–100 Hz ) oscillations in the brain often arise as a result of an interaction between excitatory and inhibitory cell populations . For this mechanism to work , the interaction must be sufficiently strong , and connectivity and external drives to participating neurons must be sufficiently homogeneous...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biology", "neuroscience" ]
2012
Minimal Size of Cell Assemblies Coordinated by Gamma Oscillations
Cerebrospinal fluid flow is crucial for neurodevelopment and homeostasis of the ventricular system of the brain , with localized flow being established by the polarized beating of the ependymal cell ( EC ) cilia . Here , we report a homozygous one base-pair deletion , c . 1193delT ( p . Leu398Glnfs*2 ) , in the Kinesin...
Cerebrospinal fluid flow is crucial for neurodevelopment and homeostasis of the ventricular system of the brain . Localized flows of cerebrospinal fluid throughout the ventricular system of the brain are established from the polarized beating of the ependymal cell ( EC ) cilia . Here , we identified a homozygous trunca...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "methods" ]
[ "fish", "medicine", "and", "health", "sciences", "body", "fluids", "kinesins", "nervous", "system", "vertebrates", "animals", "animal", "models", "osteichthyes", "mutation", "model", "organisms", "molecular", "motors", "experimental", "organism", "systems", "molecular",...
2018
Mutations in Kinesin family member 6 reveal specific role in ependymal cell ciliogenesis and human neurological development
UTX ( KDM6A ) and UTY are homologous X and Y chromosome members of the Histone H3 Lysine 27 ( H3K27 ) demethylase gene family . UTX can demethylate H3K27; however , in vitro assays suggest that human UTY has lost enzymatic activity due to sequence divergence . We produced mouse mutations in both Utx and Uty . Homozygou...
Trimethylation at Lysine 27 of histone H3 ( H3K27me3 ) establishes a repressive chromatin state in silencing an array of crucial developmental genes . Polycomb repressive complex 2 ( PRC2 ) catalyzes this precise posttranslational modification and is required in several critical aspects of development including Hox gen...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genetic", "mutation", "cell", "differentiation", "histone", "modification", "animal", "models", "developmental", "biology", "model", "organisms", "epigenetics", "embryology", "genomic", "imprinting", "gene", "expression", "biology", "mouse", "molecular", "biology", "gene...
2012
UTX and UTY Demonstrate Histone Demethylase-Independent Function in Mouse Embryonic Development
When a perturbation is applied in a sensorimotor transformation task , subjects can adapt and maintain performance by either relying on sensory feedback , or , in the absence of such feedback , on information provided by rewards . For example , in a classical rotation task where movement endpoints must be rotated to re...
The brain has a robust ability to adapt to external perturbations imposed on acquired sensorimotor transformations . Here , we used a mathematical model to investigate the reward-based component in sensorimotor adaptations . We show that the shape of the delivered reward signal , which in experiments is usually binary ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience", "motor", "systems", "biology", "computational", "neuroscience" ]
2014
Interference and Shaping in Sensorimotor Adaptations with Rewards
Accurate identification of mycetoma causative agent is a priority for treatment . However , current identification tools are far from being satisfactory for both reliable diagnosis and epidemiological investigations . A rapid , simple , and highly efficient molecular based method for identification of agents of black g...
Treatment of eumycetoma largely depends on the causative pathogen . Identification of mycetoma agent with phenotypic features is too limited , and physiological and biochemical techniques are laborious , time-consuming and nonspecific , whereas the currently available molecular methods based on DNA sequencing are speci...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences" ]
2014
Rapid Identification of Black Grain Eumycetoma Causative Agents Using Rolling Circle Amplification
Gene regulatory networks are composed of sub-networks that are often shared across biological processes , cell-types , and organisms . Leveraging multiple sources of information , such as publicly available gene expression datasets , could therefore be helpful when learning a network of interest . Integrating data acro...
Due to increasing availability of biological data , methods to properly integrate data generated across the globe become essential for extracting reproducible insights into relevant research questions . In this work , we developed a framework to reconstruct gene regulatory networks from expression datasets generated in...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "genetic", "networks", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "regulatory", "proteins", "bacillus", "microbiology", "dna-binding", "proteins", "fungi", "prokaryotic", "models", "model", "...
2019
Multi-study inference of regulatory networks for more accurate models of gene regulation
Peroxisome biogenesis disorders ( PBD ) are a group of multi-system human diseases due to mutations in the PEX genes that are responsible for peroxisome assembly and function . These disorders lead to global defects in peroxisomal function and result in severe brain , liver , bone and kidney disease . In order to study...
Peroxisomes are organelles or component of cells that are involved in body chemistry for a number of specialized fats . Peroxisome biogenesis disorders ( PBD ) are a group of rare diseases in which patients have genetic defects in the synthesis of peroxisomes . These disorders affect multiple organs including the brain...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "carbohydrate", "metabolism", "invertebrates", "glucose", "metabolism", "animals", "animal", "models", "metabolomics", "drosophila", "melanogaster", "model", "organisms", "experimental", "organism", "systems", "mitochondria", "bioenergetics", "peroxisomes", "cellular", "struc...
2017
Peroxisomal biogenesis is genetically and biochemically linked to carbohydrate metabolism in Drosophila and mouse
In comparative genomics one analyzes jointly evolutionarily related species in order to identify conserved and diverged sequences and to infer their function . While such studies enabled the detection of conserved sequences in large genomes , the evolutionary dynamics of regulatory regions as a whole remain poorly unde...
Cells use sophisticated regulation to transform static genomic information into flexible function . We are still far from understanding how such regulation evolves . Short DNA sequences that physically bind transcription factors in promoter areas near target genes play an important role in gene regulation and are direc...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "transcriptional", "regulation", "computational", "biology", "evolutionary", "biology", "evolution", "yeast" ]
2008
Evolution and Selection in Yeast Promoters: Analyzing the Combined Effect of Diverse Transcription Factor Binding Sites
Currently there are few studies characterising the nature and aetiology of human schistosome-related inflammatory processes . The aim of this study was to determine the relationship between Chitinase 3-like 1 ( CHI3L1 ) , also known as YKL-40 , a molecule associated with inflammatory processes , and schistosome infecti...
Over 100 million people , mainly living in sub-Saharan Africa , are infected with the blood fluke Schistosoma haematobium . Morbidity includes anaemia , malnutrition , impaired cognition and physical growth as well as pathology of the urogenital tract . Urogenital morbidity is initiated by eggs laid at the site of infe...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "immunopathology", "medicine", "infectious", "diseases", "schistosomiasis", "public", "health", "and", "epidemiology", "epidemiology", "infectious", "disease", "epidemiology", "neglected", "tropical", "diseases", "immunology", "biology", "parasitic", "diseases", "immune", "...
2012
Chitinase 3-Like 1 Protein Levels Are Elevated in Schistosoma haematobium Infected Children
As malignant transformation requires synchronization of growth-driving signaling ( S ) and metabolic ( M ) pathways , defining cancer-specific S-M interconnected networks ( SMINs ) could lead to better understanding of oncogenic processes . In a systems-biology approach , we developed a mathematical model for SMINs in ...
Complex and highly dynamic interconnected networks allow cancer to take different routes and circumvent chemotherapy . Therefore , understanding these context-specific networks and their dynamics of molecular interactions driven by different oncogenic signaling and metabolic pathways is very much needed to predict drug...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "protein", "interaction", "networks", "signaling", "networks", "protein", "expression", "network", "analysis", "molecular", "biology", "techniques", "research", "and", "analysis", "methods", "computer", "and", "information", "sciences", "oncogenic", "signaling", "proteins...
2019
Connecting signaling and metabolic pathways in EGF receptor-mediated oncogenesis of glioblastoma
Viral infection triggers induction of type I interferons ( IFNs ) , which are critical mediators of innate antiviral immune response . Mediator of IRF3 activation ( MITA , also called STING ) is an adapter essential for virus-triggered IFN induction pathways . How post-translational modifications regulate the activity ...
Virus infection induces the host cells to produce type I interferons , which are secreted proteins important for the host to clear viruses . Previously , we identified a cellular protein called MITA , which is essential for virus-triggered induction of interferons . In this study , we found an enzyme called RNF26 could...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "disease", "immunology", "medicine", "and", "health", "sciences", "clinical", "immunology", "biology", "and", "life", "sciences", "immunology" ]
2014
RNF26 Temporally Regulates Virus-Triggered Type I Interferon Induction by Two Distinct Mechanisms
The establishment of apical-basolateral polarity is important for both normal development and disease , for example , during tumorigenesis and metastasis . During this process , polarity complexes are targeted to the apical surface by a RAB11A-dependent mechanism . Huntingtin ( HTT ) , the protein that is mutated in Hu...
In the adult mammary gland , tissue architecture is maintained through the regulation of the polarity of epithelial cells , which organize around a central cavity called the lumen . The mammary epithelium comprises a basal layer , which contains myoepithelial contractile cells and so-called mammary stem cells , and a l...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Huntingtin Is Required for Epithelial Polarity through RAB11A-Mediated Apical Trafficking of PAR3-aPKC
Rift Valley fever ( RVF ) is a zoonotic arboviral disease that is a threat to human health , animal health and production , mainly in Sub-Saharan Africa . RVF virus dynamics have been poorly studied due to data scarcity . On the island of Mayotte in the Indian Ocean , off the Southeastern African coast , RVF has been p...
Rift Valley fever ( RVF ) is a viral zoonotic disease mainly present in Sub-Saharan Africa , transmitted by mosquitoes and primarily affecting livestock ( cattle , sheep and goats ) . The epidemiology of the disease is not fully known , mainly because of data scarcity . In Mayotte , an island close to Madagascar , RVF ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "livestock", "medicine", "and", "health", "sciences", "rift", "valley", "fever", "virus", "pathology", "and", "laboratory", "medicine", "ruminants", "infectious", "disease", "epidemiology", "pathogens", "tropical", "diseases", "microbiology", "vertebrates", "animals", "...
2016
The Epidemiology of Rift Valley Fever in Mayotte: Insights and Perspectives from 11 Years of Data
The pacific islands of Micronesia have experienced several outbreaks of mosquito-borne diseases over the past decade . In outbreaks on small islands , the susceptible population is usually well defined , and there is no co-circulation of pathogens . Because of this , analysing such outbreaks can be useful for understan...
Dengue and Zika are related viruses that are transmitted by the same species of mosquitoes . While dengue is well described and has affected people around the world for a long time , Zika has only recently caused outbreaks in human populations . To investigate whether the expected behaviour of Zika is similar to that o...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "death", "rates", "invertebrates", "dengue", "virus", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "infectious", "disease", "epidemiology", "demography", "pathogens", "vector-borne", "diseases", "microbiology", "animals", "viruses...
2016
Comparative Analysis of Dengue and Zika Outbreaks Reveals Differences by Setting and Virus
Mammals show a wide range of brain sizes , reflecting adaptation to diverse habitats . Comparing interareal cortical networks across brains of different sizes and mammalian orders provides robust information on evolutionarily preserved features and species-specific processing modalities . However , these networks are s...
It was recently shown that the network of connections between different areas of the macaque cortex has strong structural specificity in terms of the strength of connections as a function of the distance between areas . This has led to a model of cortex connectivity that predicts many observed architectural features , ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infographics", "medicine", "and", "health", "sciences", "neural", "networks", "nervous", "system", "vertebrates", "mice", "neuroscience", "animals", "mammals", "primates", "network", "analysis", "old", "world", "monkeys", "computer", "and", "information", "sciences", ...
2016
Spatial Embedding and Wiring Cost Constrain the Functional Layout of the Cortical Network of Rodents and Primates
The natural reservoir of Influenza A is waterfowl . Normally , waterfowl viruses are not adapted to infect and spread in the human population . Sometimes , through reassortment or through whole host shift events , genetic material from waterfowl viruses is introduced into the human population causing worldwide pandemic...
Influenza A's natural reservoir is waterfowl . Sometimes avian virus genomic segments are able to shift to a human host , either in toto or by combining with those that underwent a previous host shift event . Such host shift events can cause worldwide pandemics in their immunologically naive hosts . In order for these ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "evolutionary", "biology/microbial", "evolution", "and", "genomics", "virology/virus", "evolution", "and", "symbiosis", "computational", "biology/comparative", "sequence", "analysis", "molecular", "biology/molecular", "evolution", "computational", "biology/evolutionary", "modelin...
2009
Identifying Changes in Selective Constraints: Host Shifts in Influenza
Many viral infections , including HIV , exhibit sex-based pathogenic differences . However , few studies have examined vaccine-related sex differences . We compared immunogenicity and protective efficacy of monomeric SIV gp120 with oligomeric SIV gp140 in a pre-clinical rhesus macaque study and explored a subsequent se...
Viral infections can have different disease courses in men and women . Following HIV infection , women generally exhibit lower viral loads and higher CD4 counts than men , but paradoxically progress faster to AIDS . Sex differences result from effects of X-linked genes and hormonal influences , and are believed to be l...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Mucosal B Cells Are Associated with Delayed SIV Acquisition in Vaccinated Female but Not Male Rhesus Macaques Following SIVmac251 Rectal Challenge
Kaposi's sarcoma-associated herpesvirus ( KSHV ) interacts with human dermal endothelial cell surface tyrosine kinase EphrinA2 ( EphA2 ) and integrins ( α3β1 and αVβ3 ) in the lipid raft ( LR ) region , and EphA2 regulates macropinocytic virus entry by coordinating integrin-c-Cbl associated signaling . In contrast , KS...
KSHV is etiologically associated with Kaposi's sarcoma and primary effusion B-cell lymphoma . To initiate its in vitro infection of endothelial cells , KSHV interacts with cell surface heparan sulfate , integrins , and EphrinA2 ( EphA2 ) molecules in the lipid raft ( LR ) regions , which induces the integrin-c-Cbl asso...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "virology", "biology", "microbiology", "viral", "diseases", "viral", "replication" ]
2013
EphrinA2 Regulates Clathrin Mediated KSHV Endocytosis in Fibroblast Cells by Coordinating Integrin-Associated Signaling and c-Cbl Directed Polyubiquitination
Paramyxovirus hemagglutinin-neuraminidase ( HN ) plays roles in viral entry and maturation , including binding to sialic acid receptors , activation of the F protein to drive membrane fusion , and enabling virion release during virus budding . HN can thereby directly influence virulence and in a subset of avirulent New...
Newcastle disease virus ( NDV ) can cause severe disease in birds , with the most virulent strains causing sudden death , even in vaccinated populations in the poultry industry . Highly virulent exotic NDV ( END ) strains have caused largescale outbreaks in the US in 1971 and 2003 , requiring the culling of 12 million ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "viral", "enzymes", "microbiology", "host-pathogen", "interaction", "viral", "structure", "protein", "folding", "virulence", "factors", "and", "mechanisms", "proteins", "biology", "pathogenesis", "biophysics", "biochemistry", "protein", "chemistry", "viral", "envelope", "...
2012
Structure of the Ulster Strain Newcastle Disease Virus Hemagglutinin-Neuraminidase Reveals Auto-Inhibitory Interactions Associated with Low Virulence
Through their domestication and subsequent selection , sheep have been adapted to thrive in a diverse range of environments . To characterise the genetic consequence of both domestication and selection , we genotyped 49 , 034 SNP in 2 , 819 animals from a diverse collection of 74 sheep breeds . We find the majority of ...
During the process of domestication , mankind recruited animals from the wild into a captive environment , changing their morphology , behaviour , and genetics . In the case of sheep , domestication and subsequent selection by their animal handlers over thousands of years has produced a spectrum of breeds specialised f...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome", "analysis", "tools", "genomics", "genetics", "population", "genetics", "biology", "evolutionary", "biology", "evolutionary", "processes", "genetics", "and", "genomics" ]
2012
Genome-Wide Analysis of the World's Sheep Breeds Reveals High Levels of Historic Mixture and Strong Recent Selection
Increased chronic immune activation and inflammation are hallmarks of HIV/SIV infection and are highly correlated with progression to AIDS and development of non-AIDS comorbidities , such as hypercoagulability and cardiovascular disease . Intestinal dysfunction resulting in microbial translocation has been proposed as ...
We report that administration of the intraluminal antibiotic Rifaximin and the gut-focused anti-inflammatory drug Sulfasalazine to acutely SIV-infected pigtailed macaques is associated with a transient disruption of the vicious circle of inflammation-microbial translocation-immune activation which is pathognomonic to p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2016
Antibiotic and Antiinflammatory Therapy Transiently Reduces Inflammation and Hypercoagulation in Acutely SIV-Infected Pigtailed Macaques
Signaling pathways that control the activities in non-photosynthetic plastids , important sites of plant metabolism , are largely unknown . Previously , we demonstrated that WRKY2 and WRKY34 transcription factors play an essential role in pollen development downstream of mitogen-activated protein kinase 3 ( MPK3 ) and ...
Plastids are important sites of plant metabolism including fatty acid and starch biosynthesis . At present , how the activities in the plastids are coordinated with those in the cytoplasm and the signaling pathway ( s ) involved are largely unknown . Previously , we demonstrated that WRKY2 and WRKY34 transcription fact...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "fluorescence", "imaging", "plant", "anatomy", "plastids", "gene", "regulation", "plant", "cell", "biology", "regulatory", "proteins", "brassica", "dna-binding", "proteins", "pollen", "plant", "science", "model", "organisms", "experimental", "organism", "systems", "tran...
2018
Regulation of pollen lipid body biogenesis by MAP kinases and downstream WRKY transcription factors in Arabidopsis
To gain insight into female-to-male HIV sexual transmission and how male circumcision protects against this mode of transmission , we visualized HIV-1 interactions with foreskin and penile tissues in ex vivo tissue culture and in vivo rhesus macaque models utilizing epifluorescent microscopy . 12 foreskin and 14 cadave...
Although several clinical trials have demonstrated that male circumcision can protect men from becoming infected with HIV , we know very little about how men get infected through sex and how circumcision changes this . In this study , we explored possible sites of virus transmission across the penis by looking at how H...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Visualization of HIV-1 Interactions with Penile and Foreskin Epithelia: Clues for Female-to-Male HIV Transmission
Cis-regulatory modules that drive precise spatial-temporal patterns of gene expression are central to the process of metazoan development . We describe a new computational strategy to annotate genomic sequences based on their “pattern generating potential” and to produce quantitative descriptions of transcriptional reg...
The developmental program specifying segmentation along the anterior-posterior axis of the Drosophila embryo is one of the best studied examples of transcriptional regulatory networks . Previous work has identified the location and function of dozens of DNA segments called cis-regulatory “modules” that regulate several...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "developmental", "biology", "computational", "biology/transcriptional", "regulation" ]
2010
Quantitative Analysis of the Drosophila Segmentation Regulatory Network Using Pattern Generating Potentials
A sustained outbreak of leptospirosis occurred in northeast Thailand between 1999 and 2003 , the basis for which was unknown . A prospective study was conducted between 2000 and 2005 to identify patients with leptospirosis presenting to Udon Thani Hospital in northeast Thailand , and to isolate the causative organisms ...
A sustained outbreak of human leptospirosis occurred in northeast Thailand between 1999 and 2003 , the basis for which was unknown . Leptospirosis is a potentially serious infection cause by bacteria known as Leptospira; infection usually occurs following environmental exposure to pathogenic Leptospira shed in the urin...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/bacterial", "infections" ]
2007
A Dominant Clone of Leptospira interrogans Associated with an Outbreak of Human Leptospirosis in Thailand
The power of forward genetics in yeast is the foundation on which the field of autophagy research firmly stands . Complementary work on autophagy in higher eukaryotes has revealed both the deep conservation of this process , as well as novel mechanisms by which autophagy is regulated in the context of development , imm...
Eukaryotic cells use autophagy to eliminate unwanted structures—such as protein aggregates , intracellular pathogens , and damaged organelles—that are too large to be handled by the proteasome . This unusual vesicle transport pathway begins with packaging of cytoplasmic targets into a double-membrane vesicle ( autophag...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "flow", "cytometry", "genome", "engineering", "cell", "death", "autophagic", "cell", "death", "methods", "and", "resources", "vesicles", "engineering", "and", "technology", "synthetic", "biology", "cell", "processes", "green", "fluorescent", "protein", "synthetic", "b...
2019
CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor
When multiple samples are taken from the neoplastic tissues of a single patient , it is natural to compare their mutation content . This is often done by bulk genotyping of whole biopsies , but the chance that a mutation will be detected in bulk genotyping depends on its local frequency in the sample . When the underly...
Researchers who take multiple samples from a cancer or pre-cancer tissue and find that some samples show far more mutations than others are likely to conclude that the high-mutation samples reflect cells with an abnormal mutation or growth rate . We considered the common practice of testing a bulk sample for mutations ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "taxonomy", "medicine", "and", "health", "sciences", "biopsy", "cloning", "surgical", "and", "invasive", "medical", "procedures", "phylogenetics", "data", "management", "phylogenetic", "analysis", "gastroenterology", "and", "hepatology", "molecular", "biology", "technique...
2016
Bulk Genotyping of Biopsies Can Create Spurious Evidence for Hetereogeneity in Mutation Content
Thermodynamics dictates the structure and function of metabolism . Redox reactions drive cellular energy and material flow . Hence , accurately quantifying the thermodynamics of redox reactions should reveal design principles that shape cellular metabolism . However , only few redox potentials have been measured , and ...
Redox reactions define the energetic constraints within which life can exist . However , measurements of reduction potentials are scarce and unstandardized , and current prediction methods fall short of desired accuracy and coverage . Here , we harness quantum chemistry tools to enable the high-throughput prediction of...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "quantum", "chemistry", "chemical", "compounds", "density", "functional", "theory", "enzymes", "enzymology", "organic", "compounds", "carboxylic", "acids", "quantum", "mechanics", "thermodynamics", "oxidation-reduction", "reactions", "proteins", "acids", "oxidoreductases", ...
2018
Quantum chemistry reveals thermodynamic principles of redox biochemistry
We have carried out a comprehensive analysis of the determinants of human influenza A H3 hemagglutinin evolution . We consider three distinct predictors of evolutionary variation at individual sites: solvent accessibility ( as a proxy for protein fold stability and/or conservation ) , Immune Epitope Database ( IEDB ) e...
The influenza virus is one of the most rapidly evolving human viruses . Every year , it accumulates mutations that allow it to evade the host immune response of previously infected individuals . Which sites in the virus’ genome allow this immune escape and the manner of escape is not entirely understood , but conventio...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Geometric Constraints Dominate the Antigenic Evolution of Influenza H3N2 Hemagglutinin
Phenotypic variation between individuals of a species is often under quantitative genetic control . Genomic analysis of gene expression polymorphisms between individuals is rapidly gaining popularity as a way to query the underlying mechanistic causes of variation between individuals . However , there is little direct ...
Natural genetic variation and the resulting phenotypic variation between individuals within a species have been of longstanding interest in wide-ranging fields . However , the molecular underpinnings of this phenotypic variation are relatively uncharted . Recently , genomics methodologies have been applied to understan...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "biochemistry", "plant", "biology", "arabidopsis", "computational", "biology", "plants", "genetics", "and", "genomics" ]
2007
Linking Metabolic QTLs with Network and cis-eQTLs Controlling Biosynthetic Pathways
Trypanosoma cruzi , the protozoan causative of Chagas disease , is classified into six main Discrete Typing Units ( DTUs ) : TcI-TcVI . This parasite has around 105 copies of the minicircle hypervariable region ( mHVR ) in their kinetoplastic DNA ( kDNA ) . The genetic diversity of the mHVR is virtually unknown . Howev...
Chagas disease is an important public health problem in Latin America showing a wide diversity of clinical manifestations and epidemiological patterns . It is caused by the parasite Trypanosoma cruzi . This parasite is genetically diverse and classified into six main lineages . However , the relationship between intra-...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Accession", "numbers", "Results", "Discussion" ]
[ "taxonomy", "medicine", "and", "health", "sciences", "parasitic", "diseases", "parasitic", "protozoans", "phylogenetics", "data", "management", "protozoans", "mitochondria", "molecular", "biology", "techniques", "bioenergetics", "cellular", "structures", "and", "organelles"...
2019
Elucidating diversity in the class composition of the minicircle hypervariable region of Trypanosoma cruzi: New perspectives on typing and kDNA inheritance
Cell fusion in genetically identical Neurospora crassa germlings and in hyphae is a highly regulated process involving the activation of a conserved MAP kinase cascade that includes NRC-1 , MEK-2 and MAK-2 . During chemotrophic growth in germlings , the MAP kinase cascade members localize to conidial anastomosis tube (...
Cell fusion between genetically identical cells of the fungus Neurospora crassa occurs when germinating asexual cells ( conidia ) sense each other's proximity and redirect their growth . Chemotropic growth is dependent upon the assembly of a MAPK cascade ( NRC-1/MEK-2/MAK-2 ) at the cell cortex ( conidial anastomosis t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "fungal", "biochemistry", "signal", "transduction", "mycology", "mitogenic", "signaling", "fungi", "cell", "biology", "neurospora", "crassa", "fungal", "genetics", "genetics", "biology", "and", "life", "sciences", "molecular", "genetics", "molecular", "cell", "biology",...
2014
HAM-5 Functions As a MAP Kinase Scaffold during Cell Fusion in Neurospora crassa
Protein S-palmitoylation , a hydrophobic post-translational modification , is performed by protein acyltransferases that have a common DHHC Cys-rich domain ( DHHC proteins ) , and provides a regulatory switch for protein membrane association . In this work , we analyzed the presence of DHHC proteins in the protozoa par...
Giardiasis is a major cause of non-viral/non-bacterial diarrheal disease worldwide and has been included within the WHO Neglected Disease Initiative since 2004 . Infection begins with the ingestion of Giardia lamblia in cyst form , which , after exposure to gastric acid in the host stomach and proteases in the duodenum...
[ "Abstract", "Introduction", "Methods", "Results", "and", "Discussion" ]
[ "biology", "and", "life", "sciences", "parasitology", "microbiology" ]
2014
Identification of Giardia lamblia DHHC Proteins and the Role of Protein S-palmitoylation in the Encystation Process
Since its emergence in 2007 in Micronesia and Polynesia , the arthropod-borne flavivirus Zika virus ( ZIKV ) has spread in the Americas and the Caribbean , following first detection in Brazil in May 2015 . The risk of ZIKV emergence in Europe increases as imported cases are repeatedly reported . Together with chikungun...
In May 2015 , local transmission of Zika virus ( ZIKV ) was reported in Brazil and since then , more than 1 . 5 million human cases have been reported in Latin America and the Caribbean . This arbovirus , primarily found in Africa and Asia , is mainly transmitted by Aedes mosquitoes , Aedes aegypti and Aedes albopictus...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "togaviruses", "chikungunya", "infection", "pathogens", "geographical", "locations", "microbiology", "tropical", "diseases", "saliva", "animals", "alp...
2016
Zika Virus, a New Threat for Europe?
Iran , despite its size , geographic location and past cultural influence , has largely been a blind spot for human population genetic studies . With only sparse genetic information on the Iranian population available , we pursued its genome-wide and geographic characterization based on 1021 samples from eleven ethnic ...
Based on genome-wide genotype data on over 1000 samples from eleven ethnic groups present in Iran and by comparison to reference data sets of both extant populations and ancient DNA samples , we show that the Iranian population comprises distinct genetic variation with respect to populations in close geographic proximi...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "methods" ]
[ "population", "genetics", "geographical", "locations", "social", "sciences", "neuroscience", "ancient", "dna", "ethnicities", "cognitive", "psychology", "paleontology", "dna", "population", "biology", "paleogenetics", "persian", "people", "arabic", "people", "language", "...
2019
Distinct genetic variation and heterogeneity of the Iranian population
Recommendations for soil-transmitted helminth ( STH ) control give a key role to deworming of school and pre-school age children with albendazole or mebendazole; which might be insufficient to achieve adequate control , particularly against Strongyloides stercoralis . The impact of preventive chemotherapy ( PC ) agains...
Soil-transmitted helminth ( STH ) infections are a relevant public health problem in resource restricted settings due to their potential to perpetuate poverty , since chronic infections are associated with learning and grow impairment in children and reduced productivity in adults . The current strategy for STH control...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "children", "medicine", "and", "health", "sciences", "helminths", "tropical", "diseases", "hookworms", "parasitic", "diseases", "anemia", "animals", "health", "care", "age", "groups", "neglected", "tropical", "diseases", "strongyloides", "stercoralis", ...
2017
Albendazole and ivermectin for the control of soil-transmitted helminths in an area with high prevalence of Strongyloides stercoralis and hookworm in northwestern Argentina: A community-based pragmatic study
B cells can contribute to acquired immunity against intracellular bacteria , but do not usually participate in primary clearance . Here , we examined the endogenous CD4 T cell response to genital infection with Chlamydia muridarum using MHC class-II tetramers . Chlamydia-specific CD4 T cells expanded rapidly and persis...
Sexually transmitted infections caused by Chlamydia are increasing every year in the US and an effective vaccine is urgently required . Unfortunately , we currently only have a rudimentary understanding of the natural host immune response to Chlamydia infection , especially in the context of the female genital tract . ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
B Cells Enhance Antigen-Specific CD4 T Cell Priming and Prevent Bacteria Dissemination following Chlamydia muridarum Genital Tract Infection
Eye movements affect object localization and object recognition . Around saccade onset , briefly flashed stimuli appear compressed towards the saccade target , receptive fields dynamically change position , and the recognition of objects near the saccade target is improved . These effects have been attributed to differ...
Early in the vertebrate lineage fast movements of the eye , called saccades , developed . This improvement in spatial direction selectivity has been achieved at a cost to handle a sequence of different views . Recent experiments showed that the brain uses its knowledge about the upcoming eye movement to guide perceptio...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "physiology", "neuroscience", "homo", "(human)", "primates" ]
2008
The Peri-Saccadic Perception of Objects and Space
Glycosylation is a fundamental modification of proteins and membrane lipids . Toxins that utilize glycans as their receptors have served as powerful tools to identify key players in glycosylation processes . Here , we carried out Clustered Regularly Interspaced Short Palindromic Repeats ( CRISPR ) -Cas9–mediated genome...
Shiga and Shiga-like toxins ( Stxs ) are a family of bacterial toxins and key virulence factors for the bacteria Shigella dysenteriae and enterohemorrhagic Escherichia coli ( EHEC ) , which cause food poisoning throughout the world . Ricin is a plant toxin and a potential bioterrorism agent . Stxs recognize the host re...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "binding", "glycolipids", "cell", "physiology", "medicine", "and", "health", "sciences", "methods", "and", "resources", "toxins", "cell", "viability", "testing", "pathology", "and", "laboratory", "medicine", "sphingolipids", "toxic", "agents", "toxicology", "r...
2018
Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation
Bacteria can arrest their own growth and proliferation upon nutrient depletion and under various stressful conditions to ensure their survival . However , the molecular mechanisms responsible for suppressing growth and arresting the cell cycle under such conditions remain incompletely understood . Here , we identify po...
The duplication of genetic material is a prerequisite for cellular growth and proliferation . Under optimal growth conditions , when cells strive to grow and divide , DNA replication must be initiated with high frequency . However , under nutrient limiting conditions cells stop initiating DNA replication to ensure cell...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Nutritional Control of DNA Replication Initiation through the Proteolysis and Regulated Translation of DnaA
Faithful execution of developmental gene expression programs occurs at multiple levels and involves many different components such as transcription factors , histone-modification enzymes , and mRNA processing proteins . Recent evidence suggests that nucleoporins , well known components that control nucleo-cytoplasmic t...
Development of multicellular organisms such as humans requires appropriate activation of gene expression programs according to stages of differentiation . Many proteins that directly regulate this process have been identified , including histone-modifying enzymes and transcription factors . It is not clear whether nucl...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2013
Dynamic Association of NUP98 with the Human Genome
Social distancing practices are changes in behavior that prevent disease transmission by reducing contact rates between susceptible individuals and infected individuals who may transmit the disease . Social distancing practices can reduce the severity of an epidemic , but the benefits of social distancing depend on the...
One of the easiest ways for people to lower their risk of infection during an epidemic is for them to reduce their rate of contact with infectious individuals . However , the value of such actions depends on how the epidemic progresses . Few analyses of behavior change to date have accounted for how changes in behavior...
[ "Abstract", "Introduction", "Model", "Results", "Discussion" ]
[ "infectious", "diseases/epidemiology", "and", "control", "of", "infectious", "diseases" ]
2010
Game Theory of Social Distancing in Response to an Epidemic
DNA topology has fundamental control over the ability of transcription factors to access their target DNA sites at gene promoters . However , the influence of DNA topology on protein–DNA and protein–protein interactions is poorly understood . For example , relaxation of DNA supercoiling strongly induces the well-studie...
DNA is often considered to be a passive carrier of genetic information , but in fact DNA is an active participant in coordinating the expression of the genes it carries . This is because DNA is a dynamic molecule that can assume a wide range of topologies , and this has a direct impact on the formation of the protein–D...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "nucleic", "acids", "proteins", "gene", "expression", "genetics", "dna", "biology", "molecular", "genetics", "microbiology", "genetics", "and", "genomics", "bacterial", "pathogens" ]
2012
A Fundamental Regulatory Mechanism Operating through OmpR and DNA Topology Controls Expression of Salmonella Pathogenicity Islands SPI-1 and SPI-2
In many settings , copying , learning from or assigning value to group behavior is rational because such behavior can often act as a proxy for valuable returns . However , such herd behavior can also be pathologically misleading by coaxing individuals into behaviors that are otherwise irrational and it may be one sourc...
In this study we examine the neural substrates of inter-personal error signals on behavior in an investment task using real historical markets . We show that behaviorally , subjects correlate their investments , despite the fact that another trader has no extra information about how the market may move . These behavior...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Keeping up with the Joneses: Interpersonal Prediction Errors and the Correlation of Behavior in a Tandem Sequential Choice Task
Chromatin structure plays an important role in modulating the accessibility of genomic DNA to regulatory proteins in eukaryotic cells . We performed an integrative analysis on dozens of recent datasets generated by deep-sequencing and high-density tiling arrays , and we discovered an array of well-positioned nucleosome...
The accessibility of genomic DNA to regulatory proteins and to the transcriptional machinery plays an important role in eukaryotic transcription regulation . Some regulatory proteins alter chromatin structures by evicting histones in selected loci . Nonetheless , no regulatory proteins have been reported to position nu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/chromosome", "biology", "computational", "biology/transcriptional", "regulation" ]
2008
The Insulator Binding Protein CTCF Positions 20 Nucleosomes around Its Binding Sites across the Human Genome
An important goal of systems medicine is to study disease in the context of genetic and environmental perturbations to the human interactome network . For diseases with both genetic and infectious contributors , a key postulate is that similar perturbations of the human interactome by either disease mutations or pathog...
Cellular function and behaviour are driven by highly coordinated biomolecular interaction networks . A prime example is the protein-protein interaction network , often simply referred to as the “interactome” . Recent advances in systems biology have spawned the view of human disease as a manifestation of genetic and en...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "microbial", "mutation", "interaction", "networks", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "viruses", "and", "cancer", "pathogens", "cancer", "risk", "factors", "microbiology", "retroviruses", "viruses", "immunodeficiency", ...
2019
Convergent perturbation of the human domain-resolved interactome by viruses and mutations inducing similar disease phenotypes
Louse-borne relapsing fever ( LBRF ) borreliosis is caused by Borrelia recurrentis , and it is a deadly although treatable disease that is endemic in the Horn of Africa but has epidemic potential . Research on LBRF has been severely hampered because successful infection with B . recurrentis has been achieved only in pr...
Research on Borrelia recurrentis , the agent of louse-borne relapsing fever ( LBRF ) , has been hampered by the lack of a feasible non-primate animal model . By using immunocompromised SCID mice deficient in B- and T-cells , we were able to establish a stable , persistent B . recurrentis infection with low spirochetemi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/bacterial", "infections", "infectious", "diseases/neglected", "tropical", "diseases", "infectious", "diseases/tropical", "and", "travel-associated", "diseases", "microbiology/immunity", "to", "infections" ]
2009
A Novel Animal Model of Borrelia recurrentis Louse-Borne Relapsing Fever Borreliosis Using Immunodeficient Mice
Angiogenesis plays a key role in tumor growth and cancer progression . TIE-2-expressing monocytes ( TEM ) have been reported to critically account for tumor vascularization and growth in mouse tumor experimental models , but the molecular basis of their pro-angiogenic activity are largely unknown . Moreover , differenc...
Tumor vascularization is essential for tumor growth and cancer progression . In breast cancer , monocytes are angiogenic , i . e . able to induce tumor vascularization . In patients , blood circulating monocytes drastically increase their angiogenic activity when reaching the tumor , suggesting that the tumor microenvi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Angiogenic Activity of Breast Cancer Patients’ Monocytes Reverted by Combined Use of Systems Modeling and Experimental Approaches
Drosophila leg morphogenesis occurs under the control of a relatively well-known genetic cascade , which mobilizes both cell signaling pathways and tissue-specific transcription factors . However , their cross-regulatory interactions , deployed to refine leg patterning , remain poorly characterized at the gene expressi...
Limb morphogenesis is controlled by a well-known genetic cascade , mobilizing both cell signaling and tissue-specific transcription factors ( TFs ) . However , how their concerted action refines gene expression remains to be deciphered . It is thus crucial to understand how cell signaling inputs are integrated by trans...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "legs", "gene", "regulation", "regulatory", "proteins", "dna-binding", "proteins", "limbs", "(anatomy)", "cloning", "transcription", "factors", "sequence", "motif", "analysis", "molecular", "biology", "techniques", "research", "a...
2017
Tissue-specific enhancer repression through molecular integration of cell signaling inputs
During Saccharomyces cerevisiae mating-type switching , an HO endonuclease-induced double-strand break ( DSB ) at MAT is repaired by recombining with one of two donors , HMLα or HMRa , located at opposite ends of chromosome III . MATa cells preferentially recombine with HMLα; this decision depends on the Recombination ...
Mating-type gene switching occurs by a DSB–initiated gene conversion event using one of two donors , HML or HMR . MATa cells preferentially recombine with HML whereas MATα cells choose HMR . Donor preference is governed by the Recombination Enhancer ( RE ) , located about 17 kb from HML . RE is repressed in MATα cells ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "model", "organisms", "genetics", "biology", "microbiology", "molecular", "cell", "biology", "biophysics", "genetics", "and", "genomics" ]
2012
Regulation of Budding Yeast Mating-Type Switching Donor Preference by the FHA Domain of Fkh1
For all animals , the taste sense is crucial to detect and avoid ingesting toxic molecules . Many toxins are synthesized by plants as a defense mechanism against insect predation . One example of such a natural toxic molecule is l-canavanine , a nonprotein amino acid found in the seeds of many legumes . Whether and how...
Plants evolve to fend off the insects that attack them , often by synthesizing compounds toxic to insects . In turn , insects develop strategies to avoid these plants or resist their toxins . Some plant toxins are nonprotein amino acids . For example , seeds from numerous legumes contain high amounts of l-canavanine , ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/behavioral", "neuroscience", "neuroscience/sensory", "systems", "cell", "biology/neuronal", "signaling", "mechanisms", "genetics", "and", "genomics/gene", "function", "pharmacology" ]
2009
Plant Insecticide L-Canavanine Repels Drosophila via the Insect Orphan GPCR DmX
Motor tics are a cardinal feature of Tourette syndrome and are traditionally associated with an excess of striatal dopamine in the basal ganglia . Recent evidence increasingly supports a more articulated view where cerebellum and cortex , working closely in concert with basal ganglia , are also involved in tic producti...
Tourette syndrome is a neuropsychiatric disorder characterized by vocal and motor tics . Tics represent a cardinal symptom traditionally associated with a dysfunction of the basal ganglia leading to an excess of the dopamine neurotransmitter . This view gives a restricted clinical picture and limits therapeutic approac...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "neuropsychiatric", "disorders", "medicine", "and", "health", "sciences", "neurochemistry", "chemical", "compounds", "dopaminergics", "brain", "vertebrates", "neuroscience", "organic", "compounds", "animals", "mammals", "hormones", "primates", "amines", "neurotransmitters", ...
2017
Dysfunctions of the basal ganglia-cerebellar-thalamo-cortical system produce motor tics in Tourette syndrome
Mycobacterium ulcerans is the causative agent of Buruli ulcer ( BU ) . This nontuberculous mycobacterial infection has been reported in 34 countries worldwide . In Australia , the majority of cases of BU have been recorded in coastal Victoria and the Mossman-Daintree areas of north Queensland . Mosquitoes have been pos...
The causative agent of Buruli ulcer is Mycobacterium ulcerans . This destructive skin disease is characterized by extensive and painless necrosis of skin and underlying tissues usually on extremities of body due to production of toxin named mycolactone . The disease is prevalent in Africa and coastal Australia . The ex...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion", "Conclusions" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "population", "genetics", "geographical", "locations", "tropical", "diseases", "australia", "animals", "gene", "pool", "bacterial", "diseases", "signs", "and", "sympt...
2019
A survey on Mycobacterium ulcerans in Mosquitoes and March flies captured from endemic areas of Northern Queensland, Australia
How small heat shock proteins ( sHsps ) might empower proteostasis networks to control beneficial prions or disassemble pathological amyloid is unknown . Here , we establish that yeast sHsps , Hsp26 and Hsp42 , inhibit prionogenesis by the [PSI+] prion protein , Sup35 , via distinct and synergistic mechanisms . Hsp42 p...
Amyloid fibers are protein aggregates that are associated with numerous neurodegenerative diseases , including Parkinson's disease , for which there are no effective treatments . They can also play beneficial roles; in yeast , for example , they are associated with increased survival and the evolution of new traits . A...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "protein", "chemistry", "proteins", "protein", "folding", "macromolecular", "assemblies", "biology", "biophysics", "neuroscience" ]
2012
Small Heat Shock Proteins Potentiate Amyloid Dissolution by Protein Disaggregases from Yeast and Humans
Plague is a life-threatening disease caused by the bacterium , Yersinia pestis . Since the 1990s , Africa has accounted for the majority of reported human cases . In Uganda , plague cases occur in the West Nile region , near the border with Democratic Republic of Congo . Despite the ongoing risk of contracting plague i...
Plague , a severe and often fatal zoonotic disease , is caused by the bacterium Yersinia pestis . Currently , the majority of human cases have been reported from resource limited areas of Africa , where the proximity to commensal rats and other small mammals increases the likelihood for human contact with infected anim...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "plagues", "pathology", "and", "laboratory", "medicine", "respiratory", "infections", "pathogens", "geographical", "locations", "microbiology", "uganda", "vertebrates", "animals", "pulmonology", "mammals", "bacterial...
2016
Two Distinct Yersinia pestis Populations Causing Plague among Humans in the West Nile Region of Uganda
Within the liver a single Plasmodium parasite transforms into thousands of blood-infective forms to cause malaria . Here , we use RNA-sequencing to identify host genes that are upregulated upon Plasmodium berghei infection of hepatocytes with the hypothesis that host pathways are hijacked to benefit parasite developmen...
Plasmodium parasites undergo an obligatory morphogenesis and replication within the liver before they invade red blood cells and cause malaria . The liver stage is clinically silent but essential for the Plasmodium parasite to complete its life cycle . During this time , the parasite relies on the host cell to support ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "parasite", "groups", "liver", "plasmodium", "gene", "regulation", "parasitic", "diseases", "parasitic", "protozoans", "parasitology", "developmental", "biology", "apicomplexa", "protozoans", "red", "blood", "ce...
2018
Plasmodium parasite exploits host aquaporin-3 during liver stage malaria infection
The ability to generate new meaning by rearranging combinations of meaningless sounds is a fundamental component of language . Although animal vocalizations often comprise combinations of meaningless acoustic elements , evidence that rearranging such combinations generates functionally distinct meaning is lacking . Her...
A major question in language evolution is how its generative power emerged . This power , which allows the communication of limitless thoughts and ideas , is a result of the combinatorial nature of human language: meaningless phonemes can be combined to form meaningful words ( phonology ) , and words can be combined to...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Experimental Evidence for Phonemic Contrasts in a Nonhuman Vocal System
The intersection of genome-wide association analyses with physiological and functional data indicates that variants regulating islet gene transcription influence type 2 diabetes ( T2D ) predisposition and glucose homeostasis . However , the specific genes through which these regulatory variants act remain poorly charac...
Genetic studies have uncovered many different parts of the genome playing a role in the risk of developing diabetes , or affecting blood sugar levels in the normal population . However , it has so far been difficult to tie these parts of the genome to genes that are responsible for the observed changes in risk and/or b...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Transcript Expression Data from Human Islets Links Regulatory Signals from Genome-Wide Association Studies for Type 2 Diabetes and Glycemic Traits to Their Downstream Effectors
Two surgical options are available for cystic echinococcosis ( CE ) . The two principal approaches are radical ( resection of the cyst ) and conservative ( evacuation of the cyst content and partial removal of the cyst capsule ) . Here , we describe a standardized endocystectomy technique for hepatic echinococcosis . T...
Cystic echinococcosis ( CE ) is a parasitic disease caused by ingestion of the larval stage of Echinococcus granulosus . The liver is the most commonly infected organ . There are currently four treatments for CE: surgery , percutaneous treatment , medical treatment ( benzimidazoles ) , and watch-and-wait strategy . Tre...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "diagnostic", "radiology", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "bile", "parasitic", "diseases", "surgical", "and", "invasive", "medical", "procedures", "magnetic", "resonance", ...
2019
Standardized endocystectomy technique for surgical treatment of uncomplicated hepatic cystic echinococcosis
Gene tree topologies have proven a powerful data source for various tasks , including species tree inference and species delimitation . Consequently , methods for computing probabilities of gene trees within species trees have been developed and widely used in probabilistic inference frameworks . All these methods assu...
Species trees depict how species split and diverge . Within the branches of a species tree , gene trees , which depict the evolutionary histories of different genomic regions in the species , grow . Evolutionary analyses of the genomes of closely related organisms have highlighted the phenomenon that gene trees may dis...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "genetics", "biology", "computational", "biology", "evolutionary", "biology", "population", "biology", "genetics", "and", "genomics" ]
2012
The Probability of a Gene Tree Topology within a Phylogenetic Network with Applications to Hybridization Detection
During transcription , the nascent pre-mRNA undergoes a series of processing steps before being exported to the cytoplasm . The 3′-end processing machinery involves different proteins , this function being crucial to cell growth and viability in eukaryotes . Here , we found that the rna14-1 , rna15-1 , and hrp1-5 allel...
DNA damage occurs constantly in living cells and needs to be recognized and repaired to avoid mutations . DNA repair is particularly relevant for lesions occurring in actively transcribed DNA strands because the RNA polymerase cannot proceed through a damaged site . Stalled RNA polymerases and persisting DNA lesions ca...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cellular", "stress", "responses", "model", "organisms", "rna", "processing", "dna", "replication", "nucleic", "acids", "gene", "expression", "genetics", "gene", "regulation", "molecular", "genetics", "yeast", "and", "fungal", "models", "biology", "saccharomyces", "ce...
2014
Cleavage Factor I Links Transcription Termination to DNA Damage Response and Genome Integrity Maintenance in Saccharomyces cerevisiae
Hox genes in species across the metazoa encode transcription factors ( TFs ) containing highly-conserved homeodomains that bind target DNA sequences to regulate batteries of developmental target genes . DNA-bound Hox proteins , together with other TF partners , induce an appropriate transcriptional response by RNA Poly...
Mutations of Hox developmental genes in the fruit fly Drosophila melanogaster may provoke spectacular changes in form: transformations of one body part into another , or loss of organs . This attribute identifies them as important developmental genes . Insect and vertebrate Hox proteins contain highly related homeodoma...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "animal", "genetics", "protein", "interactions", "enzymology", "dna-binding", "proteins", "animals", "cell", "differentiation", "dna", "transcription", "animal", "models", "developmental", "biology", "drosophila", "melanogaster", "model", "organisms", "orga...
2014
Drosophila melanogaster Hox Transcription Factors Access the RNA Polymerase II Machinery through Direct Homeodomain Binding to a Conserved Motif of Mediator Subunit Med19
Bacterial and human voltage-gated sodium channels ( Navs ) exhibit similar cation selectivity , despite their distinct EEEE and DEKA selectivity filter signature sequences . Recent high-resolution structures for bacterial Navs have allowed us to learn about ion conduction mechanisms in these simpler homo-tetrameric cha...
Ion channels can rapidly and selectively conduct an ionic species , essential for the firing of neurons , where sodium and potassium channels respond to changes in membrane potential to release stores of sodium and potassium ions in succession . The ability of a protein pore to discriminate between these two nearly ide...
[ "Abstract", "Introduction", "Methods", "Results", "and", "discussion" ]
[ "medicine", "and", "health", "sciences", "chemical", "compounds", "electricity", "electrophysiology", "neuroscience", "organic", "compounds", "simulation", "and", "modeling", "ion", "channels", "electrostatics", "basic", "amino", "acids", "amino", "acids", "thermodynamics...
2018
Selective ion permeation involves complexation with carboxylates and lysine in a model human sodium channel
The gene encoding the GroEL chaperonin is duplicated in nearly 30% of bacterial genomes; and although duplicated groEL genes have been comprehensively determined to have distinct physiological functions in different species , the mechanisms involved have not been characterized to date . Myxococcus xanthus DK1622 has tw...
GroEL is a type I chaperonin , involved in protein folding , assembly , and transport . It is a major group of heat-shock proteins that are over-expressed at high temperatures and has fundamental roles in growth and survival at non-permissive temperatures . Because of its importance in many cellular processes , the gro...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genetics", "biology", "evolutionary", "biology", "genomic", "evolution", "genetics", "and", "genomics" ]
2013
Mechanisms Involved in the Functional Divergence of Duplicated GroEL Chaperonins in Myxococcus xanthus DK1622
Zinc finger MYND-type-containing 10 ( ZMYND10 ) , a cytoplasmic protein expressed in ciliated cells , causes primary ciliary dyskinesia ( PCD ) when mutated; however , its function is poorly understood . Therefore , in this study , we examined the roles of ZMYND10 using Zmynd10–/–mice exhibiting typical PCD phenotypes ...
Dynein arm defects are linked to primary ciliary dyskinesia ( PCD ) . ZMYND10 increased the stability of its interacting proteins and specifically regulated intermediate chain protein assembly , revealing tightly regulated mechanisms underlying dynein arm assembly and PCD-related pathogenesis . Increasing protein stabi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "molecular", "probe", "techniques", "immunoblotting", "dyneins", "molecular", "motors", "respiratory", "system", "immunoprecipitation", "co-immunoprecipitation", "molecular", "biology", "techniques", "trachea", "immunologic", "techniqu...
2018
ZMYND10 stabilizes intermediate chain proteins in the cytoplasmic pre-assembly of dynein arms
The ubiquitin-proteolytic system controls the stability of proteins in space and time . In this study , using a temperature-sensitive mutant allele of the cul-2 gene , we show that CRL2LRR-1 ( CUL-2 RING E3 ubiquitin-ligase and the Leucine Rich Repeat 1 substrate recognition subunit ) acts at multiple levels to control...
Maintenance of the germline depends on the presence of a germline stem cell pool with self-renewal potential that produces gametes upon meiotic differentiation . Factors regulating the balance between germline stem cell self-renewal and meiotic differentiation ensure germline homeostasis , whereas disruption of these r...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "germ", "cells", "meiosis", "molecular", "cell", "biology", "cell", "biology", "chromosome", "biology", "cellular", "types", "biology", "genomics", "genetics", "and", "genomics" ]
2013
CRL2LRR-1 E3-Ligase Regulates Proliferation and Progression through Meiosis in the Caenorhabditis elegans Germline
Post-transcriptional regulation of gene expression plays a crucial role in many bacterial pathways . In particular , the translation of mRNA can be regulated by trans-acting , small , non-coding RNAs ( sRNAs ) or mRNA-binding proteins , each of which has been successfully treated theoretically using two-component model...
Gene expression is a fundamental biological process , in which living cells use genetic information to synthesize functional products like proteins . To control this process , cells make use of many different mechanisms . A well-studied example is the binding of expression intermediates by a cellular component in order...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "dimers", "(chemical", "physics)", "medicine", "and", "health", "sciences", "toxins", "pathology", "and", "laboratory", "medicine", "engineering", "and", "technology", "gene", "regulation", "signal", "processing", "messenger", "rna", "signal", "filtering", "toxic", "a...
2016
Hierarchical Post-transcriptional Regulation of Colicin E2 Expression in Escherichia coli
The INSIG2 rs7566605 polymorphism was identified for obesity ( BMI≥30 kg/m2 ) in one of the first genome-wide association studies , but replications were inconsistent . We collected statistics from 34 studies ( n = 74 , 345 ) , including general population ( GP ) studies , population-based studies with subjects selecte...
A polymorphism of the INSIG2 gene was identified as being associated with obesity in one of the first genome-wide association studies . However , this association has since then been highly debated upon inconsistent subsequent reports . We collected association information from 34 studies including a total of 74 , 000 ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "public", "health", "and", "epidemiology" ]
2009
Meta-Analysis of the INSIG2 Association with Obesity Including 74,345 Individuals: Does Heterogeneity of Estimates Relate to Study Design?
Mouse taste receptor cells survive from 3–24 days , necessitating their regeneration throughout adulthood . In anterior tongue , sonic hedgehog ( SHH ) , released by a subpopulation of basal taste cells , regulates transcription factors Gli2 and Gli3 in stem cells to control taste cell regeneration . Using single-cell ...
Adult taste cell regeneration is essential for maintaining peripheral taste cells throughout life . The Shh pathway is an important regulator of taste bud development and regeneration in both embryonic and adult stages . We show that the transcription factor Gli3 , an important effector of the Shh pathway , is expresse...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "taste", "buds", "organoids", "medicine", "and", "health", "sciences", "chemical", "compounds", "biological", "cultures", "social", "sciences", "vertebrates", "mice", "neuroscience", "animals", "mammals", "tongue", "organ", "cultures", "digestive", "system", "research",...
2018
Gli3 is a negative regulator of Tas1r3-expressing taste cells
There is growing evidence that molecular subtypes ( e . g . luminal and basal subtypes ) affect the prognosis and treatment response in patients with muscle invasive urinary bladder cancer ( invasive urothelial carcinoma , iUC ) . Modeling these subtypes in pre-clinical animal studies is essential , but it is challengi...
Approximately 50% of patients with invasive urinary bladder cancer ( invasive urothelial carcinoma , “iUC” ) die from their cancer . Better therapeutic strategies are essential . A relatively recent important finding in iUC is the presence of molecular subtypes ( luminal and basal subtypes ) . These subtypes affect the...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "sequencing", "techniques", "urology", "medicine", "and", "health", "sciences", "cancer", "treatment", "immunology", "clinical", "oncology", "cancers", "and", "neoplasms", "vertebrates", "genitourinary", "tract", "tumors", "cancer", "immunotherapy", "dogs", "animals", "...
2018
Naturally-occurring canine invasive urothelial carcinoma harbors luminal and basal transcriptional subtypes found in human muscle invasive bladder cancer
The BXD genetic reference population is a recombinant inbred panel descended from crosses between the C57BL/6 ( B6 ) and DBA/2 ( D2 ) strains of mice , which segregate for about 5 million sequence variants . Recently , some of these variants have been established with effects on general metabolic phenotypes such as glu...
Using 43 strains from the BXD mouse reference population , we observed a 5-fold difference in spontaneous activity . QTL analysis indicated that ∼40% of this variance is due to the aryl hydrocarbon receptor ( Ahr ) . Ahr is a conserved transcription factor found in nearly all multicellular organisms and implicated in a...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genome", "expression", "analysis", "animal", "genetics", "functional", "genomics", "quantitative", "trait", "loci", "population", "genetics", "quantitative", "traits", "diet", "mutation", "nutrition", "genome", "analysis", "trait", "locus", "analysis", "missense", "mut...
2014
An Evolutionarily Conserved Role for the Aryl Hydrocarbon Receptor in the Regulation of Movement
Worldwide the mosquito Aedes aegypti ( L . ) is the principal urban vector of dengue viruses . Currently 2 . 5 billion people are at risk for infection and reduction of Ae . aegypti populations is the most effective means to reduce the risk of transmission . Pyrethroids are used extensively for adult mosquito control ,...
Constant use of pyrethroid insecticides has driven mosquito populations to develop resistance . In Aedes aegypti , the primary mosquito vector of dengue , yellow Fever , and chikungunya viruses , pyrethroid resistance is primarily associated with mutations in the voltage-gated sodium channel protein . One mutation occu...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Coevolution of the Ile1,016 and Cys1,534 Mutations in the Voltage Gated Sodium Channel Gene of Aedes aegypti in Mexico
Malaria parasites must undergo a round of sexual reproduction in the blood meal of a mosquito vector to be transmitted between hosts . Developing a transmission-blocking intervention to prevent parasites from mating is a major goal of biomedicine , but its effectiveness could be compromised if parasites can compensate ...
Malaria and related parasites cause some of the most serious infectious diseases of humans , domestic animals and wildlife . To be transmitted , these parasites produce male and female sexual stages that differentiate into gametes and mate when taken up in a mosquito blood meal . Despite the need to develop a transmiss...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "evolutionary", "biology/evolutionary", "ecology", "immunology/innate", "immunity", "immunology", "infectious", "diseases/protozoal", "infections", "evolutionary", "biology", "immunology/immunity", "to", "infections" ]
2011
Sex and Death: The Effects of Innate Immune Factors on the Sexual Reproduction of Malaria Parasites
Campylobacter jejuni is one of the leading infectious causes of food-borne illness around the world . Its ability to persistently colonize the intestinal tract of a broad range of hosts , including food-producing animals , is central to its epidemiology since most infections are due to the consumption of contaminated f...
There is accumulating evidence that in addition to canonical virulence factors such as toxins , adhesins , or invasins , bacterial pathogens utilize specific metabolic traits to colonize and proliferate within their hosts , a concept that is increasingly referred to as “nutritional virulence” . We have used transposon ...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "dna-binding", "proteins", "microbiology", "campylobacter", "jejuni", "genetic", "elements", "molecular", "biology", "techniques", "bacteria", "campylobacter", "bacterial", ...
2017
Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni
Schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected . Development of a vaccine is thought to be the most effective way to control this disease . Recombinant 26-kDa glutathione S-transferase ( rSjGST ) has previously been reported to achieve ...
Schistosomiasis continues to be a serious global public health problem that considered by World Health Organization ( WHO ) . More than 200 million people are infected and cause 280 thousand deaths every year . Among , Schistosoma japonicum causes the most severe pathological damages and the slowest immune resistance m...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "schistosoma", "invertebrates", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "cytokines", "helminths", "granulomas", "immunology", "animals", "vaccines", "preventive", "medicine", "developmental", "biolo...
2016
Combined IL-12 Plasmid and Recombinant SjGST Enhance the Protective and Anti-pathology Effect of SjGST DNA Vaccine Against Schistosoma japonicum
During echinoderm development , expression of nodal on the right side plays a crucial role in positioning of the rudiment on the left side , but the mechanisms that restrict nodal expression to the right side are not known . Here we show that establishment of left-right asymmetry in the sea urchin embryo relies on reci...
Asymmetries between the left and the right sides of the body are an essential feature of most bilaterian animals , and failure to establish these asymmetries can result in pathological disorders in humans . Left-right asymmetries are established during early development by the asymmetric activity of a signaling pathway...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "evolutionary", "biology", "biology", "pattern", "formation", "evolutionary", "developmental", "biology" ]
2012
Reciprocal Signaling between the Ectoderm and a Mesendodermal Left-Right Organizer Directs Left-Right Determination in the Sea Urchin Embryo
The misexpressed imprinted genes causing developmental failure of mouse parthenogenones are poorly defined . To obtain further insight , we investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome ( Chr ) 7 ( MatDup . dist7 ) . The...
Parthenogenetic mouse embryos with two maternal genomes die early in development due to the misexpression of imprinted genes . To gain further insight into which misexpressions might be involved , we examined some of the misexpressions that could determine the small size and fetal death of a “partial parthenogenone”—em...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "expression", "developmental", "biology/developmental", "evolution", "genetics", "and", "genomics/epigenetics", "developmental", "biology/developmental", "molecular", "mechanisms", "evolutionary", "biology/developmental", "evolution" ]
2010
Postnatal Survival of Mice with Maternal Duplication of Distal Chromosome 7 Induced by a Igf2/H19 Imprinting Control Region Lacking Insulator Function
Lethal recessive alleles cause pre- or postnatal death in homozygous affected individuals , reducing fertility . Especially in small size domestic and wild populations , those alleles might be exposed by inbreeding , caused by matings between related parents that inherited the same recessive lethal allele from a common...
Lethal recessives are mutations that cause early lethality in homozygous state that usually occur at very low frequency in wild and domestic populations . In livestock , however , those mutations might become more prevalent as a result of inbreeding . In this study , we report five such recessive lethal haplotypes that...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "population", "genetics", "vertebrates", "animals", "mammals", "alleles", "genetic", "mapping", "mutation", "population", "biology", "frameshift", "mutation", "swine", "genetic", "loci", "genetic", "drift", "haplotypes", "heterosis", "eukaryota", "heredity", "genetics", ...
2019
Loss of function mutations in essential genes cause embryonic lethality in pigs
During prophase of the first meiotic division ( prophase I ) , chromatin dynamically reorganises to recombine and prepare for chromosome segregation . Histone modifying enzymes are major regulators of chromatin structure , but our knowledge of their roles in prophase I is still limited . Here we report on crucial roles...
Accurate transmission of chromosomes carrying genetic materials from generation to generation is essential for life . Cell divisions that generate gametes , such as eggs and sperm , are critical , as chromosomes inherited from both parents recombine and are accurately sorted into gametes . Errors in these cell division...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "homologous", "chromosomes", "reproductive", "system", "rna", "interference", "chromosome", "structure", "and", "function", "centromeres", "cell", "cycle", "and", "cell", "division", "cell", "processes", "germ", "cells", "oocyt...
2016
Kdm5/Lid Regulates Chromosome Architecture in Meiotic Prophase I Independently of Its Histone Demethylase Activity
H2A . Z is a histone H2A variant conserved from yeast to humans , and is found at 63% of promoters in Saccharomyces cerevisiae . This pattern of localization suggests that H2A . Z is somehow important for gene expression or regulation . H2A . Z can be acetylated at up to four lysine residues on its amino-terminal tail ...
Transcriptional memory is the well-documented phenomenon by which cells can “remember” prior transcriptional states . A paradigmatic example of transcriptional memory is found in the yeast Saccharomyces . S . cerevisiae remembers prior transcription of the galactose metabolism gene GAL1 . When a gene is transcribed , t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/gene", "expression", "genetics", "and", "genomics/gene", "expression" ]
2010
Roles for H2A.Z and Its Acetylation in GAL1 Transcription and Gene Induction, but Not GAL1-Transcriptional Memory
The case-fatality rate of severe leptospirosis can exceed 50% . While prompt supportive care can improve survival , predicting those at risk of developing severe disease is challenging , particularly in settings with limited diagnostic support . We retrospectively identified all adults with laboratory-confirmed leptosp...
Leptospirosis , a neglected tropical disease with a global distribution , is estimated to kill 60 , 000 people every year . Predicting those at risk of developing severe disease is challenging , and a simple scoring system to quantify the risk of severe disease has proven elusive . Identifying the high-risk patient is ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "clinical", "laboratory", "sciences", "pathology", "and", "laboratory", "medicine", "respiratory", "infections", "tropical", "diseases", "geographical", "locations", "australia", "biomarkers", "pulmonology", "bacterial", "diseases", ...
2019
A simple score to predict severe leptospirosis
Natively unstructured or disordered regions appear to be abundant in eukaryotic proteins . Many such regions have been found alongside small linear binding motifs . We report a Monte Carlo study that aims to elucidate the role of disordered regions adjacent to such binding motifs . The coarse-grained simulations show t...
In their natural cellular environment proteins are dissolved in a concentrated aqueous solution of biomolecules . Even under such crowded conditions , proteins must not clump together or aggregate; otherwise their biological functions may be compromised , and the cell could die . Diseases such as Parkinson and Alzheime...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biophysics/protein", "folding", "biophysics/theory", "and", "simulation", "biophysics/biomacromolecule-ligand", "interactions", "computational", "biology", "chemical", "biology/protein", "chemistry", "and", "proteomics" ]
2008
Disordered Flanks Prevent Peptide Aggregation
Exosomes are secreted organelles that have the same topology as the cell and bud outward ( outward is defined as away from the cytoplasm ) from endosome membranes or endosome-like domains of plasma membrane . Here we describe an exosomal protein-sorting pathway in Jurkat T cells that selects cargo proteins on the basis...
Exosomes are small , secreted organelles with the same topology as the cell and a similar size and composition as retrovirus particles . Based on these similarities , we proposed that retroviruses are , at their most fundamental level , exosomes . Little is known about the mechanisms of exosome biogenesis . We show her...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "viruses", "infectious", "diseases", "cell", "biology", "virology", "microbiology" ]
2007
Higher-Order Oligomerization Targets Plasma Membrane Proteins and HIV Gag to Exosomes
Behavioral output of neural networks depends on a delicate balance between excitatory and inhibitory synaptic connections . However , it is not known whether network formation and stability is constrained by the sign of synaptic connections between neurons within the network . Here we show that switching the sign of a ...
Fast neurotransmission in the nervous system is mediated by ligand-gated ion channels . Within the nervous system , the sign of synaptic connections , i . e . , whether they are excitatory or inhibitory , is determined by the charge of the ions that flow through these channels . In general , channels that conduct posit...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
A Change in the Ion Selectivity of Ligand-Gated Ion Channels Provides a Mechanism to Switch Behavior
The traditional cardiac model-building paradigm involves constructing a composite model using data collected from many cells . Equations are derived for each relevant cellular component ( e . g . , ion channel , exchanger ) independently . After the equations for all components are combined to form the composite model ...
Mathematical models of cardiac cell electrophysiology are widely used as predictive and illuminatory tools , but have been developed for decades using a suboptimal process . The models are typically constructed by manual adjustment of parameters to fit simple data and therefore often underperform when used to predict c...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Cell-Specific Cardiac Electrophysiology Models
Candida albicans is a normal resident of the gastrointestinal tract and also the most prevalent fungal pathogen of humans . It last shared a common ancestor with the model yeast Saccharomyces cerevisiae over 300 million years ago . We describe a collection of 143 genetically matched strains of C . albicans , each of wh...
A key goal in the understanding of the biology of an organism is the description of the regulatory networks that control the expression of its genes . Changes in gene expression result in new cellular phenotypes that can be acted upon by evolutionary forces to influence the configuration of these networks . We have dev...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "expression", "evolutionary", "biology/evolutionary", "and", "comparative", "genetics", "infectious", "diseases/fungal", "infections", "genetics", "and", "genomics/gene", "function", "evolutionary", "biology/morphogenesis", "and", "cell", "...
2009
A Phenotypic Profile of the Candida albicans Regulatory Network
To better understand genome regulation , it is important to uncover the role of transcription factors in the process of chromatin structure establishment and maintenance . Here we present a data-driven approach to systematically characterise transcription factors that are relevant for this process . Our method uses a l...
Transcription factor binding occurs mainly in regions of open chromatin . For many transcription factors , it is unclear whether binding is the cause or the consequence of open chromatin . Here , we used datasets on open chromatin and gene expression provided by the ENCODE project to predict which transcription factors...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "gene", "regulation", "regulatory", "proteins", "dna-binding", "proteins", "random", "variables", "covariance", "regression", "analysis", "regulator", "genes", "mathematics", "statistics", "(mathematics)", "transcription", "factors", "gene", "types", "epigenetics", "chromat...
2017
Genome-Wide Association between Transcription Factor Expression and Chromatin Accessibility Reveals Regulators of Chromatin Accessibility
Plant growth depends on stem cell niches in meristems . In the root apical meristem , the quiescent center ( QC ) cells form a niche together with the surrounding stem cells . Stem cells produce daughter cells that are displaced into a transit-amplifying ( TA ) domain of the root meristem . TA cells divide several time...
Plant roots are programmed to grow continuously into the soil , searching for nutrients and water . The iterative process of cell division , elongation , and differentiation contributes to root growth . The quiescent center ( QC ) is known to maintain the root meristem , and thus ensure root growth . In this study , we...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
PHABULOSA Controls the Quiescent Center-Independent Root Meristem Activities in Arabidopsis thaliana
B-cells , in addition to antibody secretion , have emerged increasingly as effector and immunoregulatory cells in several chronic inflammatory diseases . Although Erythema Nodosum Leprosum ( ENL ) is an inflammatory complication of leprosy , the role of B- cell subsets has never been studied in this patient group . The...
Some leprosy patients develop reactions which cause a significant morbidity and mortality in leprosy patients . There are two types of leprosy reactions , type 1 and type 2 reactions . Type 2 or Erythema nodosum leprosum ( ENL ) is an immune-mediated inflammatory complication of leprosy which occurs in lepromatous and ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "immunology", "tropical", "diseases", "bacterial", "diseases", "b", "cell", "receptors", "neglected", "tropical", "disea...
2017
Increased activated memory B-cells in the peripheral blood of patients with erythema nodosum leprosum reactions
Cell size increases significantly with increasing ploidy . Differences in cell size and ploidy are associated with alterations in gene expression , although no direct connection has been made between cell size and transcription . Here we show that ploidy-associated changes in gene expression reflect transcriptional adj...
Cells of the same type , whether microbial , plant , or metazoan in origin , exhibit remarkable uniformity in size . This uniformity arises from control mechanisms that respond to internal cellular changes as well as external environmental factors . Although precise control of cell size is a universal phenomenon , its ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology", "cell", "biology/gene", "expression", "microbiology" ]
2010
Control of Transcription by Cell Size
While it is appreciated that population size changes can impact patterns of deleterious variation in natural populations , less attention has been paid to how gene flow affects and is affected by the dynamics of deleterious variation . Here we use population genetic simulations to examine how gene flow impacts deleteri...
Individuals from distinct populations sometimes will produce fertile offspring and will exchange genetic material in a process called hybridization . Genomes of hybrid individuals often show non-random patterns of hybrid ancestry across the genome , where some regions have a high frequency of ancestry from the second p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "biotechnology", "deletion", "mutation", "engineering", "and", "technology", "population", "genetics", "brassica", "simulation", "and", "modeling", "mutation", "plant", "science", "model", "organisms", "experimental", "organism", "systems", "plant", "genomics", "populatio...
2018
Deleterious variation shapes the genomic landscape of introgression
The dynamic of cancer is intimately linked to a dysregulation of the cell cycle and signalling pathways . It has been argued that selectivity of treatments could exploit loss of checkpoint function in cancer cells , a concept termed “cyclotherapy” . Quantitative approaches that describe these dysregulations can provide...
Neoplastic transformation results from mutations , chromosomal abnormalities , or expression changes affecting components of the cell cycle , the signalling pathways leading into it , and the apoptosis pathways resulting from cell cycle arrest . Cytotoxic agents , but also newer drugs that target the cell cycle and its...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "death", "medicine", "and", "health", "sciences", "cell", "cycle", "and", "cell", "division", "cancer", "treatment", "cell", "processes", "departures", "from", "diploidy", "oncology", "pharmacodynamics", "pharmacology", "synthesis", "phase", "aneuploidy", "sig...
2017
Modelling of the cancer cell cycle as a tool for rational drug development: A systems pharmacology approach to cyclotherapy
Cellulosic plant biomass is a promising sustainable resource for generating alternative biofuels and biochemicals with microbial factories . But a remaining bottleneck is engineering microbes that are tolerant of toxins generated during biomass processing , because mechanisms of toxin defense are only beginning to emer...
Understanding the genetic architecture of complex traits is important for elucidating the genotype-phenotype relationship . Many studies have sought genetic variants that underlie phenotypic variation across individuals , both to implicate causal variants and to inform on architecture . Here we used genome-wide associa...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biotechnology", "genome-wide", "association", "studies", "carbohydrate", "metabolism", "medicine", "and", "health", "sciences", "toxins", "pathology", "and", "laboratory", "medicine", "glucose", "metabolism", "alleles", "toxic", "agents", "toxicology", "glucose", "tolera...
2018
Genome-wide association across Saccharomyces cerevisiae strains reveals substantial variation in underlying gene requirements for toxin tolerance
Meiotic recombination permits exchange of genetic material between homologous chromosomes . The replication protein A ( RPA ) complex , the predominant ssDNA-binding complex , is required for nearly all aspects of DNA metabolism , but its role in mammalian meiotic recombination remains unknown due to the embryonic leth...
Meiosis , a process unique to germ cells , results in production of haploid gametes . Meiotic recombination , a hallmark of meiosis , together with random segregation of homologous chromosomes , generates genetic diversity in haploid gametes at every generation so that each gamete has a unique genetic composition . Suc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "meiosis", "medicine", "and", "health", "sciences", "reproductive", "system", "spermatocytes", "cell", "cycle", "and", "cell", "division", "cell", "processes", "germ", "cells", "molecular", "biology", "techniques", "dna", "sperm", "homologous", "recombination", "resea...
2019
Dual functions for the ssDNA-binding protein RPA in meiotic recombination
Neural maps are emergent , highly ordered structures that are essential for organizing and presenting synaptic information . Within the embryonic nervous system of Drosophila motoneuron dendrites are organized topographically as a myotopic map that reflects their pattern of innervation in the muscle field . Here we rev...
During development the axons of sensory neurons generate highly ordered ”sensory maps„ within the nervous system that represent specific qualities of the environment . Much less is known about the anatomical organization and development of motor systems . Here , we show that the leg motoneurons of Drosophila organize t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/motor", "systems", "neuroscience/neurodevelopment", "developmental", "biology/neurodevelopment" ]
2009
Dendritic Targeting in the Leg Neuropil of Drosophila: The Role of Midline Signalling Molecules in Generating a Myotopic Map
Airway inflammation plays a major role in the pathogenesis of influenza viruses and can lead to a fatal outcome . One of the challenging objectives in the field of influenza research is the identification of the molecular bases associated to the immunopathological disorders developed during infection . While its precis...
Influenza A viruses may cause severe respiratory disease . PB1-F2 , a viral protein identified in 2001 is suspected to play a role in influenza-related pneumonia . In order to understand the impact of PB1-F2 in the pathogenesis underlying Influenza A virus infection , we engineered a mutant virus unable to express PB1-...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "models", "of", "infection", "inflammation", "immunity", "virology", "immune", "activation", "innate", "immunity", "immune", "defense", "biology", "microbiology", "immunoregulation" ]
2011
Transcriptomic Analysis of Host Immune and Cell Death Responses Associated with the Influenza A Virus PB1-F2 Protein
Uropathogenic E . coli ( UPEC ) is the primary cause of urinary tract infections ( UTI ) affecting approximately 150 million people worldwide . Here , we revealed the importance of transcriptional regulator hypoxia-inducible factor-1 α subunit ( HIF-1α ) in innate defense against UPEC-mediated UTI . The effects of AKB-...
Urinary tract infection ( UTI ) , commonly caused by uropathogenic E . coli ( UPEC ) , affects more than 150 million people worldwide , resulting in 14 million hospital visits per year and an estimated total cost of 6 billion dollars in direct health care . Due to the high prevalence of UTI and rapid emergence of antib...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Role of Hypoxia Inducible Factor-1α (HIF-1α) in Innate Defense against Uropathogenic Escherichia coli Infection
A scientific ontology is a formal representation of knowledge within a domain , typically including central concepts , their properties , and relations . With the rise of computers and high-throughput data collection , ontologies have become essential to data mining and sharing across communities in the biomedical scie...
An ontology represents the concepts and their interrelation within a knowledge domain . Several ontologies have been developed in biomedicine , which provide standardized vocabularies to describe diseases , genes and gene products , physiological phenotypes , anatomical structures , and many other phenomena . Scientist...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "computational", "biology/literature", "analysis", "computational", "biology/bio-ontology" ]
2011
Benchmarking Ontologies: Bigger or Better?
Rift Valley fever ( RVF ) is a zoonotic disease caused by Rift Valley fever virus ( RVFV ) found in Africa and the Middle East . Outbreaks can cause extensive morbidity and mortality in humans and livestock . Following the diagnosis of two acute human RVF cases in Kabale district , Uganda , we conducted a serosurvey to...
Viral hemorrhagic fevers are known to cause high morbidity and mortality and pose a serious threat to human and animal populations in endemic countries . An outbreak of Rift Valley fever was detected in Kabale district in March , 2016 and identified the first human cases in Uganda since 1968 . There was a need to perfo...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "livestock", "medicine", "and", "health", "sciences", "rift", "valley", "fever", "virus", "pathology", "and", "laboratory", "medicine", "ruminants", "pathogens", "geographical", "locations", "microbiology", "uganda", "vertebrates", "diet", "tropical", "...
2018
Prevalence and risk factors of Rift Valley fever in humans and animals from Kabale district in Southwestern Uganda, 2016