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Genetic similarity of spouses can reflect factors influencing mate choice , such as physical/behavioral characteristics , and patterns of social endogamy . Spouse correlations for both genetic ancestry and measured traits may impact genotype distributions ( Hardy Weinberg and linkage equilibrium ) , and therefore genet...
We analyzed three generations of whites from the Framingham Heart Study ( FHS ) using genome-wide genotype data to characterize their genetic ancestry . By examination of spouse-pairs , we observed that individuals of Northern/Western European , Southern European and Ashkenazi ancestry preferentially chose spouses of t...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "genome-wide", "association", "studies", "medicine", "and", "health", "sciences", "variant", "genotypes", "population", "genetics", "genetic", "mapping", "regression", "analysis", "linkage", "disequilibrium", "mathematics", "statistics", "(mathematics)", "genome", "analysis...
2017
Structured mating: Patterns and implications
We consider the problem of how to detect cognate pairs of proteins that bind when each belongs to a large family of paralogs . To illustrate the problem , we have undertaken a genomewide analysis of interactions of members of the PE and PPE protein families of Mycobacterium tuberculosis . Our computational method uses ...
We consider the problem of detecting protein interactions from genome sequences when the potential interacting partners belong to large families of similar ( homologous ) proteins . Many computational methods for predicting protein interactions rely on similarity to a pair of known interacting proteins . When the prote...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology/microbial", "evolution", "and", "genomics", "genetics", "and", "genomics/functional", "genomics", "microbiology/microbial", "evolution", "and", "genomics", "computational", "biology/genomics", "infectious", "diseases/bacterial", "infections", "computation...
2008
Identifying Cognate Binding Pairs among a Large Set of Paralogs: The Case of PE/PPE Proteins of Mycobacterium tuberculosis
Small molecule signaling promotes the communication between bacteria as well as between bacteria and eukaryotes . The opportunistic pathogenic bacterium Legionella pneumophila employs LAI-1 ( 3-hydroxypentadecane-4-one ) for bacterial cell-cell communication . LAI-1 is produced and detected by the Lqs ( Legionella quor...
Legionella pneumophila is a ubiquitous environmental bacterium , which upon inhalation causes a severe pneumonia termed Legionnaires’ disease . The opportunistic pathogen employs the small molecule LAI-1 ( Legionella autoinducer-1 ) for bacterial cell-cell communication . LAI-1 is produced and detected by the Lqs ( Leg...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Inter-kingdom Signaling by the Legionella Quorum Sensing Molecule LAI-1 Modulates Cell Migration through an IQGAP1-Cdc42-ARHGEF9-Dependent Pathway
Glucagon , secreted from pancreatic islet α cells , stimulates gluconeogenesis and liver glycogen breakdown . The mechanism regulating glucagon release is debated , and variously attributed to neuronal control , paracrine control by neighbouring β cells , or to an intrinsic glucose sensing by the α cells themselves . W...
Glucagon is a critical regulator of glucose homeostasis . Its major action is to mobilize glucose from the liver . Glucagon secretion from α cells of the pancreatic islets of Langerhans is suppressed by elevated blood sugar , a response that is often perturbed in diabetes . Much work has focused on the regulation of α-...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "physiology", "in", "vitro", "diabetes", "and", "endocrinology", "homo", "(human)", "rattus", "(rat)" ]
2007
A KATP Channel-Dependent Pathway within α Cells Regulates Glucagon Release from Both Rodent and Human Islets of Langerhans
The architecture of dendritic arbors determines circuit connectivity , receptive fields , and computational properties of neurons , and dendritic structure is impaired in several psychiatric disorders . While apical and basal dendritic compartments of pyramidal neurons are functionally specialized and differentially re...
A fundamental feature of a neuron is the morphology of its dendrites , which are the processes that receive and integrate synaptic signals from other neurons . Neurons in the mammalian cortex exhibit two distinct dendritic arbors: apical dendrites , which extend far from the cell body , and basal dendrites , which elab...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "neuroscience", "cellular", "neuroscience", "neuronal", "morphology", "signaling", "pathways", "biology", "neuroscience" ]
2012
An Autism-Associated Variant of Epac2 Reveals a Role for Ras/Epac2 Signaling in Controlling Basal Dendrite Maintenance in Mice
Meiotic recombination is required for the orderly segregation of chromosomes during meiosis and for providing genetic diversity among offspring . Among mammals , as well as yeast and higher plants , recombination preferentially occurs at highly delimited chromosomal sites 1–2 kb long known as hotspots . Although consid...
Recombination is an essential aspect of meiosis , ensuring proper contact and exchange of genetic material between homologous parental chromosomes , as well as their subsequent segregation to produce haploid gametes . In humans and mice , recombination events are located at preferential sites termed hotspots , whose pl...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology", "molecular", "biology", "genetics", "and", "genomics" ]
2009
Trans-Regulation of Mouse Meiotic Recombination Hotspots by Rcr1
It is now established that the central nervous system plays an important role in regulating whole body metabolism and energy balance . However , the extent to which sensory systems relay environmental information to modulate metabolic events in peripheral tissues has remained poorly understood . In addition , it has be...
The central nervous system plays a vital role in regulating whole body metabolism and energy balance . However , the precise cellular , genetic and molecular mechanisms underlying these effects remain a major unsolved mystery . C . elegans has emerged as a tractable and highly informative model to study the neurobiolog...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "biotechnology", "invertebrates", "medicine", "and", "health", "sciences", "caenorhabditis", "neuroscience", "animals", "animal", "models", "caenorhabditis", "elegans", "model", "organisms", "oils", "experimental", "organism", "systems", "genetic", "engineering", "digestive...
2017
Pheromone-sensing neurons regulate peripheral lipid metabolism in Caenorhabditis elegans
Lymphedema is a debilitating and disfiguring sequela of an overwhelmed lymphatic system . The most common causes of secondary lymphedema are lymphatic filariasis ( LF ) , a vector-borne , parasitic disease endemic in 73 tropical countries , and treatment for cancer in developed countries . Lymphedema is incurable and r...
Secondary lymphedema is a major cause of disability worldwide . The most common causes are treatment for cancer or infection with lymphatic filariasis . In both cases , the lymphatic system is damaged and unable to perform its normal function of removing extracellular fluid and wastes . Protein rich fluid accumulates i...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "legs", "pathology", "and", "laboratory", "medicine", "chemical", "compounds", "sports", "and", "exercise", "medicine", "salts", "limbs", "(anatomy)", "database", "searching", "physical", "activity", "health", "care", "signs", ...
2016
Self-Care for Management of Secondary Lymphedema: A Systematic Review
Individual rapid tests for serodiagnosis ( RDT ) of human African trypanosomiasis ( HAT ) are particularly suited for passive screening and surveillance . However , so far , no large scale evaluation of RDTs has been performed for diagnosis of Trypanosoma brucei gambiense HAT in West Africa . The objective of this stud...
Screening for gambiense human African trypanosomiasis ( HAT ) or sleeping sickness is traditionally based on detection of trypanosome specific antibodies in blood . Whereas the card agglutination test is particularly suited for mass screening , individual rapid serodiagnostic tests ( RDTs ) are rather adapted for use i...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Accuracy of Individual Rapid Tests for Serodiagnosis of Gambiense Sleeping Sickness in West Africa
Understanding emotion is critical for a science of healthy and disordered brain function , but the neurophysiological basis of emotional experience is still poorly understood . We analyzed human brain activity patterns from 148 studies of emotion categories ( 2159 total participants ) using a novel hierarchical Bayesia...
Neuroimaging provides a unique way of understanding the ‘emotional brain’ by identifying patterns across multiple systems that imbue each instance of emotion with its particular qualities . In this meta-analysis across 148 studies , we ask whether it is possible to identify patterns that differentiate five emotion cate...
[ "Abstract", "Introduction", "Results", "Network", "Co-activation", "Differences", "among", "Emotion", "Categories", "Discussion", "Methods" ]
[]
2015
A Bayesian Model of Category-Specific Emotional Brain Responses
Vertebrate hearts depend on highly specialized cardiomyocytes that form the cardiac conduction system ( CCS ) to coordinate chamber contraction and drive blood efficiently and unidirectionally throughout the organism . Defects in this specialized wiring system can lead to syncope and sudden cardiac death . Thus , a gre...
Aberrant electrical activity of the heart , otherwise known as cardiac arrhythmia , may disrupt heart contractions , leading to loss of consciousness and sudden death . Every year , approximately 450 , 000 individuals in the United States die suddenly from this event . Currently , the only proven preventive therapy for...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "cell", "biology", "genetics", "and", "genomics", "cardiovascular", "disorders" ]
2008
Genetic and Physiologic Dissection of the Vertebrate Cardiac Conduction System
Estimation of liver function is important to monitor progression of chronic liver disease ( CLD ) . A promising method is magnetic resonance imaging ( MRI ) combined with gadoxetate , a liver-specific contrast agent . For this method , we have previously developed a model for an average healthy human . Herein , we exte...
Being able to accurately and reliably estimate liver function is important when monitoring the progression of patients with liver disease , as well as when identifying drug-induced liver injury during drug development . A promising method for quantifying liver function is to use magnetic resonance imaging combined with...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "liver", "immune", "physiology", "diagnostic", "radiology", "body", "fluids", "biopsy", "spleen", "fibrosis", "liver", "diseases", "surgical", "and", "invasive", "medical", "procedures", "magnetic", "resonance", "imaging", "phy...
2019
Model-inferred mechanisms of liver function from magnetic resonance imaging data: Validation and variation across a clinically relevant cohort
Glioblastoma multiforme ( GBM ) is the most aggressive type of brain tumor . Molecular heterogeneity is a hallmark of GBM tumors that is a barrier in developing treatment strategies . In this study , we used the nonsynonymous mutations of GBM tumors deposited in The Cancer Genome Atlas ( TCGA ) and applied a systems le...
Precision medicine aims to find the best treatment strategy based on the information about the patient’s tumor . Molecular heterogeneity is the main obstacle in developing treatment strategies . Therefore , transforming patient specific molecular data into clinically interpretable knowledge is fundamental in precision ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "interaction", "networks", "protein", "interactions", "protein", "interaction", "networks", "mutation", "network", "analysis", "nonsense", "mutation", "protein", "structure", "mutation", "databases", "frameshift", "mutation", "research", "and", "analysis", "methods", "com...
2019
3D spatial organization and network-guided comparison of mutation profiles in Glioblastoma reveals similarities across patients
Recent efforts to cure human immunodeficiency virus type-1 ( HIV-1 ) infection have focused on developing latency reversing agents as a first step to eradicate the latent reservoir . The histone deacetylase inhibitor , vorinostat , has been shown to activate HIV RNA transcription in CD4+ T-cells and alter host cell gen...
Combination antiretroviral therapy ( cART ) for HIV infection must be taken for life due to the existence of long lived latently infected cells . Recent efforts have focused on developing latency reversing agents to eliminate latently infected cells by activating HIV production . In this work , we assess the impact of ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Modeling the Effects of Vorinostat In Vivo Reveals both Transient and Delayed HIV Transcriptional Activation and Minimal Killing of Latently Infected Cells
The treatment of HCV infection has seen significant progress , particularly since the approval of new direct-acting antiviral drugs . However these clinical achievements have been made despite an incomplete understanding of HCV replication and within-host evolution , especially compared with HIV-1 . Here , we undertake...
Our knowledge of HCV within-host evolution is substantially limited , which is surprising given that highly successful therapies against the virus have been developed . Key aspects of HCV infection , such as rapid fluctuations in viral diversity and the reappearance of viral strains years after their initial detection ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "taxonomy", "organismal", "evolution", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "evolutionary", "biology", "hepacivirus", "pathogens", "population", "genetics", "microbiology", "retroviruses", "viruses", "immunodeficiency", "vir...
2016
Exceptional Heterogeneity in Viral Evolutionary Dynamics Characterises Chronic Hepatitis C Virus Infection
Buruli ulcer ( BU ) is a slowly progressing , necrotising disease of the skin caused by infection with Mycobacterium ulcerans . Non-ulcerative manifestations are nodules , plaques and oedema , which may progress to ulceration of large parts of the skin . Histopathologically , BU is characterized by coagulative necrosis...
Buruli ulcer caused by Mycobacterium ulcerans infection is a necrotizing disease of the skin and the underlying subcutaneous tissue . Since the skin of pigs ( Sus scrofa ) has striking structural and physiological similarities with human skin , we investigated whether it is possible to develop an experimental M . ulcer...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "mycobacterium", "ulcerans", "bacterial", "diseases", "infectious", "diseases", "buruli", "ulcer", "medicine", "and", "health", "sciences", "anatomical", "pathology", "histopathology", "animal", "models", "of", "infection", "pathology", "and", "laboratory", "medicine", ...
2014
Experimental Infection of the Pig with Mycobacterium ulcerans: A Novel Model for Studying the Pathogenesis of Buruli Ulcer Disease
Viruses use cellular machinery to enter and infect cells . In this study we address the cell entry mechanisms of nonenveloped adenoviruses ( Ads ) . We show that protein VI , an internal capsid protein , is rapidly exposed after cell surface attachment and internalization and remains partially associated with the capsi...
Viruses exploit cellular functions during entry and exit of cells . To redirect cellular functions for their own purpose , viruses encode high-affinity binding sites for key-cellular factors . One such domain is the PPxY motif , which is present in structural proteins of several , mainly enveloped viruses . This motif ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/virion", "structure,", "assembly,", "and", "egress", "virology/host", "invasion", "and", "cell", "entry" ]
2010
A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry
Endogenous retroviruses are cellular genes of retroviral origin captured by their host during the course of evolution and represent around 8% of the human genome . Although most are defective and transcriptionally silenced , some are still able to generate retroviral-like particles and proteins . Among these , the HERV...
Nearly half the DNA of mammals consists of reitarated , selfish elements that can move and amplify within the genome . With time , some of these elements are recruited by the host and the proteins they encode are used to fulfill physiological functions , whereas other elements have conserved some of their pathological ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "transfection", "medicine", "and", "health", "sciences", "gene", "regulation", "293t", "cells", "regulatory", "proteins", "biological", "cultures", "rna", "extraction", "dna-binding", "proteins", "oncology", "transcription", "factors", "bioassays", "and...
2017
A human endogenous retrovirus-derived gene that can contribute to oncogenesis by activating the ERK pathway and inducing migration and invasion
Recent advances in epigenomics have made it possible to map genome-wide regulatory regions using empirical methods . Subsequent comparative epigenomic studies have revealed that regulatory regions diverge rapidly between genome of different species , and that the divergence is more pronounced in enhancers than in promo...
Regions of the genome that do not encode genes but affect expression of other genes , such as enhancers and promoters , are referred to as regulatory regions . Because of their regulatory functions , it was thought that enhancers and promoters should be evolutionarily conserved . Regulatory regions can be now epigenomi...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "comparative", "sequence", "analysis", "chemical", "characterization", "statistics", "vertebrates", "animals", "mammals", "mathematics", "forecasting", "sequence", "motif", "analysis", "epigenomics", "research", "and", "analysis", "methods", "sequence", "analysis", "genomic...
2018
Functional conservation of sequence determinants at rapidly evolving regulatory regions across mammals
Independently evolving populations may adapt to similar selection pressures via different genetic changes . The interactions between such changes , such as in a hybrid individual , can inform us about what course adaptation may follow and allow us to determine whether gene flow would be facilitated or hampered followin...
We crossed yeast bearing different genetic mutations to determine the fitness of their hybrid offspring . These strains had previously evolved in the presence of the fungicide nystatin . Even though the initial strains had nearly identical genomes , differing only in the mutation they carried within the biosynthetic pa...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "antimicrobials", "medicine", "and", "health", "sciences", "drugs", "population", "genetics", "microbiology", "antifungals", "epistasis", "mutation", "sign", "epistasis", "pharmacology", "population", "biology", "mycology", "lipids", "fitness", "epistasis", "mutant", "str...
2017
Widespread Genetic Incompatibilities between First-Step Mutations during Parallel Adaptation of Saccharomyces cerevisiae to a Common Environment
Light plays a critical role in the regulation of numerous aspects of physiology and behaviour , including the entrainment of circadian rhythms and the regulation of sleep . These responses involve melanopsin ( OPN4 ) -expressing photosensitive retinal ganglion cells ( pRGCs ) in addition to rods and cones . Nocturnal l...
Light exerts profound effects on our physiology and behaviour , setting our biological clocks to the correct time and regulating when we are asleep and we are awake . The photoreceptors mediating these responses include the rods and cones involved in vision , as well as a subset of photosensitive retinal ganglion cells...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "white", "light", "medicine", "and", "health", "sciences", "sleep", "vertebrates", "light", "mice", "animals", "mammals", "electromagnetic", "radiation", "physiological", "processes", "animal", "behavior", "adrenal", "glands", "visible", "light", "zoology", "behavior", ...
2016
Melanopsin Regulates Both Sleep-Promoting and Arousal-Promoting Responses to Light
Alveolar macrophages ( AM ) are critical for defense against bacterial and fungal infections . However , a definitive role of AM in viral infections remains unclear . We here report that AM play a key role in survival to influenza and vaccinia virus infection by maintaining lung function and thereby protecting from asp...
Acute respiratory viral infections can cause severe morbidity and pneumonia in infected individuals . Alveolar macrophages and various subsets of dendritic cells have been implicated in innate immunity and induction of anti-viral T cell responses that contribute to host defense against influenza virus infection . Howev...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunopathology", "immune", "cells", "cell", "biology", "animal", "cells", "clinical", "immunology", "biology", "and", "life", "sciences", "cellular", "types", "immunology" ]
2014
Alveolar Macrophages Are Essential for Protection from Respiratory Failure and Associated Morbidity following Influenza Virus Infection
Animal venoms are theorized to evolve under the significant influence of positive Darwinian selection in a chemical arms race scenario , where the evolution of venom resistance in prey and the invention of potent venom in the secreting animal exert reciprocal selection pressures . Venom research to date has mainly focu...
While the influence of positive selection in diversifying animal venoms is widely recognized , the role of purifying selection that conserves the amino acid sequence of venom components such as peptide toxins has never been considered . In addition to unraveling the unique strategies of evolution of toxin gene families...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
The Rise and Fall of an Evolutionary Innovation: Contrasting Strategies of Venom Evolution in Ancient and Young Animals
Spike-timing-dependent plasticity ( STDP ) has been observed in many brain areas such as sensory cortices , where it is hypothesized to structure synaptic connections between neurons . Previous studies have demonstrated how STDP can capture spiking information at short timescales using specific input configurations , s...
Tuning feature extraction of sensory stimuli is an important function for synaptic plasticity models . A widely studied example is the development of orientation preference in the primary visual cortex , which can emerge using moving bars in the visual field . A crucial point is the decomposition of stimuli into basic ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "neuroscience", "biology", "neuroscience" ]
2012
Spectral Analysis of Input Spike Trains by Spike-Timing-Dependent Plasticity
Inflammatory monocytes can be manipulated by environmental cues to perform multiple functions . To define the role of monocytes during primary or secondary infection with an intra-phagosomal pathogen we employed Leishmania major-red fluorescent protein ( RFP ) parasites and multi-color flow cytometry to define and enum...
Many infectious diseases are initiated in the context of inflammation . This inflammatory response may be initiated by the pathogen itself or by damage to barrier sites associated with the infectious process . In the case of the vector-transmitted intra-phagosomal pathogen Leishmania , the parasite must contend with th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "respiratory", "infections", "ears", "immunology", "parasitic", "diseases", "parasitic", "protozoans", "pulmonology", "otology", "ear", "infection...
2017
Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major
Cells are naturally surrounded by organized electrical signals in the form of local ion fluxes , membrane potential , and electric fields ( EFs ) at their surface . Although the contribution of electrochemical elements to cell polarity and migration is beginning to be appreciated , underlying mechanisms are not known ....
The ability of cells to orient towards spatial cues is critical for processes such as migration , wound healing , and development . Although the role of electrochemical signals is well characterized in processes such as neuronal signaling , their function in cell polarity is much less understood or appreciated . Applic...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biophysics", "cell", "biology", "biology", "and", "life", "sciences", "ion", "transport" ]
2014
Electrochemical Regulation of Budding Yeast Polarity
The impact of global climate change on the transmission dynamics of infectious diseases is the subject of extensive debate . The transmission of mosquito-borne viral diseases is particularly complex , with climatic variables directly affecting many parameters associated with the prevalence of disease vectors . While ev...
Although a link between the increased susceptibility of mosquitoes for arthropod-borne viruses and exposure to lower rearing temperatures has been known for many years , the molecular basis of this has remained unknown . We investigated this phenomenon using an engineered strain of mosquito where the expression of a re...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "mosquitoes", "rna", "interference", "emerging", "infectious", "diseases", "viral", "transmission", "and", "infection", "vector", "biology", "virology", "gene", "expression", "genetics", "gene", "regulation", "molecular", "genetics", "emerging", "viral", "diseases", "bi...
2013
Cooler Temperatures Destabilize RNA Interference and Increase Susceptibility of Disease Vector Mosquitoes to Viral Infection
Increasing seed oil content is one of the most important breeding goals for soybean due to a high global demand for edible vegetable oil . However , genetic improvement of seed oil content has been difficult in soybean because of the complexity of oil metabolism . Determining the major variants and molecular mechanisms...
Soybean seed oil is an important quality trait targeted during domestication and breeding . However , the molecular mechanism of soybean oil regulation is largely unknown due to its complex genetic architecture and environmental sensitivity . In this paper , we integrated GWAS across multiple environments , haplotype a...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "methods" ]
[ "genome-wide", "association", "studies", "plant", "anatomy", "quantitative", "trait", "loci", "plant", "products", "genetic", "mapping", "plant", "science", "oils", "genome", "analysis", "crops", "lipids", "genomics", "crop", "science", "gene", "expression", "seeds", ...
2019
Artificial selection on GmOLEO1 contributes to the increase in seed oil during soybean domestication
An estimated 2 million inhabitants are infected with Chagas disease in Mexico , with highest prevalence coinciding with highest demographic density in the southern half of the country . After vector-borne transmission , Trypanosoma cruzi is principally transmitted to humans via blood transfusion . Despite initiation of...
Chagas disease continues to be a neglected disease in Mexico and Latin-American . Although an estimated 96% of Trypanosoma cruzi transmission to humans occurs via 32 triatomine vector species , the only transmission prevention in Mexico has been sparse and based on heterogeneous blood donation screening . Despite manda...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "clinical", "laboratory", "sciences", "body", "fluids", "enzyme-linked", "immunoassays", "cost-effectiveness", "analysis", "economic", "analysis", "geographical", "locations", "social", "sciences", "parasitic", "protozoans", "health"...
2016
Cost-Effectiveness of Blood Donation Screening for Trypanosoma cruzi in Mexico
Triatomines are vectors of Trypanosoma cruzi , the etiological agent of Chagas disease in Latin America . The most effective vector , Triatoma infestans , has been controlled successfully in much of Latin America using insecticide spraying . Though rarely undertaken , surveillance programs are necessary in order to ide...
Chagas disease , caused by Trypanosoma cruzi , is a neglected disease with 20 million people at risk in Latin America . The main control strategies are based on insecticide spraying to eliminate the domestic vectors , the most effective of which is Triatoma infestans . This approach has been very successful in some are...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "immunology/immune", "response", "infectious", "diseases/epidemiology", "and", "control", "of", "infectious", "diseases", "biotechnology/protein", "chemistry", "and", "proteomics" ]
2009
Immunogenic Salivary Proteins of Triatoma infestans: Development of a Recombinant Antigen for the Detection of Low-Level Infestation of Triatomines
Missense mutant proteins , such as those produced in individuals with genetic diseases , are often misfolded and subject to processing by intracellular quality control systems . Previously , we have shown using a yeast system that enzymatic function could be restored to I278T cystathionine β-synthase ( CBS ) , a cause ...
Genetic diseases are often caused by missense mutations: single nucleotide changes that cause a single incorrect amino acid to be substituted into the underlying protein . Most missense mutations cause the encoded protein to fold incorrectly and therefore not function properly . Examples of missense mutation diseases i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/disease", "models", "genetics", "and", "genomics/genetics", "of", "disease", "biochemistry/protein", "folding" ]
2010
Activation of Mutant Enzyme Function In Vivo by Proteasome Inhibitors and Treatments that Induce Hsp70
Tumourigenesis within the intestine is potently driven by deregulation of the Wnt pathway , a process epigenetically regulated by the chromatin remodelling factor Brg1 . We aimed to investigate this interdependency in an in vivo setting and assess the viability of Brg1 as a potential therapeutic target . Using a range ...
Aberrant Wnt signalling is responsible for the majority of colorectal cancers , the third leading cause of cancer-related mortality in the UK . However , no therapies directly targeting Wnt signalling are currently available . Using mouse models of intestinal cancer , we demonstrate that deleting chromatin remodelling ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "signal", "transduction", "medicine", "and", "health", "sciences", "cancer", "genetics", "cell", "biology", "chromosome", "biology", "gastroenterology", "and", "hepatology", "gene", "expression", "genetics", "biology", "and", "life", "sciences", "gastrointestinal", "can...
2014
Brg1 Loss Attenuates Aberrant Wnt-Signalling and Prevents Wnt-Dependent Tumourigenesis in the Murine Small Intestine
In cases of Dengue fever , late hospital admission can lead to treatment delay and even death . In order to improve early disease notification and management , it is essential to investigate the factors affecting the time of admission of Dengue cases . This study determined the factors associated with the time of admis...
A variety of factors affect the time of admission of Dengue fever cases . These must be investigated , as delayed treatment of this disease can result in death . The authors of this study determined the factors associated with the time of admission among notified Dengue cases of Region VIII , Philippines , from 2008 to...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "elderly", "tropical", "diseases", "geographical", "locations", "health", "care", "age", "groups", "chi", "square", "tests", "adults", "mathematics", "statistics", "(mathematics)", "neglected", "tropical", "diseases", "research",...
2016
Factors Associated with the Time of Admission among Notified Dengue Fever Cases in Region VIII Philippines from 2008 to 2014
Central chemoreceptors are highly sensitive neurons that respond to changes in pH and CO2 levels . An increase in CO2/H+ typically reflects a rise in the firing rate of these neurons , which stimulates an increase in ventilation . Here , we present an ionic current model that reproduces the basic electrophysiological a...
The sensory mechanism by which changes in CO2 and H+ levels are detected in the brain is known as central chemoreception . Altered chemoreception is common to a wide variety of clinical conditions , including sleep apnea , sudden infant death syndrome , hyperventilation , depression , anxiety and asthma . In addition ,...
[ "Abstract", "Introduction", "Results", "Discussion", "Model" ]
[ "acidosis", "medicine", "and", "health", "sciences", "action", "potentials", "pathology", "and", "laboratory", "medicine", "membrane", "potential", "brain", "electrophysiology", "neuroscience", "surgical", "and", "invasive", "medical", "procedures", "signs", "and", "sym...
2017
Theoretical perspectives on central chemosensitivity: CO2/H+-sensitive neurons in the locus coeruleus
Simian immunodeficiency virus ( SIV ) infection leads to AIDS in experimentally infected macaques , whereas natural reservoir hosts exhibit limited disease and pathology . It is , however , unclear how natural hosts can sustain high viral loads , comparable to those observed in the pathogenic model , without developing...
Simian immunodeficiency virus ( SIV ) does not cause disease in African green monkeys ( a natural host for the virus ) , whereas experimentally infected Asian macaques ( a non-natural host ) develop a progressive disease that is similar to that which occurs in HIV-infected humans . Insight into how HIV causes disease a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "expression", "genetics", "and", "genomics/functional", "genomics", "virology/immunodeficiency", "viruses", "virology/animal", "models", "of", "infection", "immunology/innate", "immunity", "virology/mechanisms", "of", "resistance", "and", "...
2009
Transcriptional Profiling in Pathogenic and Non-Pathogenic SIV Infections Reveals Significant Distinctions in Kinetics and Tissue Compartmentalization
Dengue is the most prevalent arthropod-borne viral illness in humans with half of the world’s population at risk . During early infancy , severe dengue can develop after a primary dengue virus infection . There has been a clinical observation that severe dengue during the first year of life is seen only in chubby infan...
Dengue is the most prevalent arthropod-borne viral illness in humans with half of the world’s population at risk . During early infancy , severe dengue can develop after a primary dengue virus infection . There has been a clinical observation that severe dengue during the first year of life is seen only in chubby infan...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
The Pattern of Adipose Tissue Accumulation during Early Infancy Provides an Environment for the Development of Dengue Hemorrhagic Fever
Viral invasion triggers the activation of the host antiviral response . Besides the innate immune response , stress granules ( SGs ) also act as an additional defense response to combat viral replication . However , many viruses have evolved various strategies to suppress SG formation to facilitate their own replicatio...
Human parainfluenza virus type 3 ( HPIV3 ) is one of the major causes of acute respiratory tract diseases such as pneumonia and bronchitis in infants and children . Virus invasion activates cellular stress responses . One of these responses is the formation of SGs which counteract viral replication . However , many vir...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "medicine", "and", "health", "sciences", "nucleic", "acid", "synthesis", "nuclear", "staining", "hela", "cells", "biological", "cultures", "messenger", "rna", "brain", "microbiology", "red", "nucleus", "cell", "cultures", "rna", "synthesis", "chemic...
2018
Inclusion bodies of human parainfluenza virus type 3 inhibit antiviral stress granule formation by shielding viral RNAs
Urogenital schistosomiasis due to Schistosoma haematobium is a serious underestimated public health problem affecting 112 million people - particularly in sub-Saharan Africa . Microscopic examination of urine samples to detect parasite eggs still remains as definitive diagnosis . This work was focussed on developing a ...
Human schistosomiasis is a disease caused by several species of parasitic worms of the genus Schistosoma that is affecting 200 million people , especially in sub-Saharan Africa . Most people are infected with Schistosoma haematobium , the species that causes urogenital schistosomiasis and also bladder cancer in many ch...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
The Rapid-Heat LAMPellet Method: A Potential Diagnostic Method for Human Urogenital Schistosomiasis
Neuronal cargos are differentially targeted to either axons or dendrites , and this polarized cargo targeting critically depends on the interaction between microtubules and molecular motors . From a forward mutagenesis screen , we identified a gain-of-function mutation in the C . elegans α-tubulin gene mec-12 that trig...
Axons and dendrites are two classes of neuronal process that differ in their functions and molecular compositions . Proteins important for synaptic functions are mostly synthesized in the cell body and sorted differentially into the axon or dendrites . Microtubules in the axon and dendrite maintain their structural int...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "cell", "biology", "genetics", "biology", "and", "life", "sciences", "neuroscience" ]
2014
Genetic Analysis of a Novel Tubulin Mutation That Redirects Synaptic Vesicle Targeting and Causes Neurite Degeneration in C. elegans
The actions of the RIG-I like receptor ( RLR ) and type I interferon ( IFN ) signaling pathways are essential for a protective innate immune response against the emerging flavivirus West Nile virus ( WNV ) . In mice lacking RLR or IFN signaling pathways , WNV exhibits enhanced tissue tropism , indicating that specific ...
West Nile virus ( WNV ) , a mosquito-transmitted RNA flavivirus , is an NIAID Category B infectious agent that has emerged in the Western hemisphere as a serious public health threat . The innate immune effectors that impart restriction of WNV infection are not well defined . WNV infection is sensed by the host RIG-I l...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immune", "cells", "immune", "activation", "antigen-presenting", "cells", "immunity", "to", "infections", "immunology", "microbiology", "emerging", "viral", "diseases", "host-pathogen", "interaction", "mechanisms", "of", "resistance", "and", "susceptibility", "emerging", ...
2013
A Systems Biology Approach Reveals that Tissue Tropism to West Nile Virus Is Regulated by Antiviral Genes and Innate Immune Cellular Processes
While influenza viruses are a common respiratory pathogen , sporadic reports of conjunctivitis following human infection demonstrates the ability of this virus to cause disease outside of the respiratory tract . The ocular surface represents both a potential site of virus replication and a portal of entry for establish...
Most infections with influenza virus result in respiratory disease . However , influenza viruses of the H7 subtype frequently cause ocular and not respiratory symptoms during human infection , demonstrating that the eye represents an alternate location for influenza viruses to infect humans . Using a ferret model , we ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology", "biology", "microbiology" ]
2012
Influenza Virus Respiratory Infection and Transmission Following Ocular Inoculation in Ferrets
Viral infection leads to induction of pattern-recognition receptor signaling , which leads to interferon regulatory factor ( IRF ) activation and ultimately interferon ( IFN ) production . To establish infection , many viruses have strategies to evade the innate immunity . For the hepatitis B virus ( HBV ) , which caus...
Viral infection is sensed by the host innate immune system , which acts to limit viral infection by inducing antiviral cytokines such as the interferons . To establish infection , many viruses have strategies to evade the innate immunity . For the hepatitis B virus ( HBV ) , which causes chronic infection in the liver ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/viral", "replication", "and", "gene", "regulation", "virology/immune", "evasion" ]
2010
Hepatitis B Virus Polymerase Blocks Pattern Recognition Receptor Signaling via Interaction with DDX3: Implications for Immune Evasion
In both prokaryotic and eukaryotic cells , gene expression is regulated across the cell cycle to ensure “just-in-time” assembly of select cellular structures and molecular machines . However , present in all time-series gene expression measurements is variability that arises from both systematic error in the cell synch...
Time-series analyses of cellular regulatory processes have successfully drawn attention to the importance of temporal regulation in biological systems . A number of model systems can be synchronized such that data collected on cell populations better reflect the dynamic properties of the individual cell . However , exp...
[ "Abstract", "Introduction", "Model", "Results", "Discussion" ]
[ "mathematics", "computational", "biology/systems", "biology", "cell", "biology/gene", "expression" ]
2009
Model-Based Deconvolution of Cell Cycle Time-Series Data Reveals Gene Expression Details at High Resolution
The way in which single neurons transform input into output spike trains has fundamental consequences for network coding . Theories and modeling studies based on standard Integrate-and-Fire models implicitly assume that , in response to increasingly strong inputs , neurons modify their coding strategy by progressively ...
Over the last decades , a variety of simplified spiking models have been shown to achieve a surprisingly high performance in predicting the neuronal responses to in vitro somatic current injections . Because of the complex adaptive behavior featured by cortical neurons , this success is however restricted to limited st...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "action", "potentials", "medicine", "and", "health", "sciences", "membrane", "potential", "electrophysiology", "neuroscience", "systems", "science", "mathematics", "forecasting", "statistics", "(mathematics)", "computational", "neuroscience", "cellular", "structures", "and", ...
2016
Enhanced Sensitivity to Rapid Input Fluctuations by Nonlinear Threshold Dynamics in Neocortical Pyramidal Neurons
Very little is known about how vector-borne pathogens interact within their vector and how this impacts transmission . Here we show that mosquitoes can accumulate mixed strain malaria infections after feeding on multiple hosts . We found that parasites have a greater chance of establishing and reach higher densities if...
Very little is known about how malaria parasite strains interact with each other inside mosquitoes . In this study we show that mosquitoes that have already been infected with one strain of malaria parasites are more likely to become infected with a new strain . Moreover , the presence of an existing infection enhances...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Existing Infection Facilitates Establishment and Density of Malaria Parasites in Their Mosquito Vector
Insects rely primarily on innate immune responses to fight pathogens . In Drosophila , antimicrobial peptides are key contributors to host defense . Antimicrobial peptide gene expression is regulated by the IMD and Toll pathways . Bacterial peptidoglycans trigger these pathways , through recognition by peptidoglycan re...
The innate immune system controls the immediate response to infection . Innate immunity relies on germline encoded receptors , receptors that are present at birth , to recognize germs and trigger a protective response . Invertebrates ( i . e . , insects ) rely on innate immunity to survive in microbial-rich environment...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/bacterial", "infections", "immunology/genetics", "of", "the", "immune", "system", "immunology/immunity", "to", "infections", "immunology/innate", "immunity" ]
2008
Rudra Interrupts Receptor Signaling Complexes to Negatively Regulate the IMD Pathway
Most species are superbly and intricately adapted to the environments in which they live . Adaptive evolution by natural selection is the primary force shaping biological diversity . Differences between closely related species in ecologically selected characters such as habitat preference , reproductive timing , courts...
Adaptive evolution by natural selection is the primary force generating biological diversity . A critical question is whether the evolution of hybrid incompatibility , which is essential for the maintenance of species diversity , is caused by adaptive evolution . In this article , we investigate one of the most widely ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "ecology", "plant", "biology", "population", "genetics", "quantitative", "traits", "plant", "science", "linkage", "(genetics)", "plant", "genetics", "biology", "evolutionary", "genetics", "genotypes", "trait", "locus", "plant", "evolution", "phenotypes", ...
2013
Indirect Evolution of Hybrid Lethality Due to Linkage with Selected Locus in Mimulus guttatus
We study apo and holo forms of the bacterial ferric binding protein ( FBP ) which exhibits the so-called ferric transport dilemma: it uptakes iron from the host with remarkable affinity , yet releases it with ease in the cytoplasm for subsequent use . The observations fit the “conformational selection” model whereby th...
Upon binding ligands , many proteins undergo structural changes compared to the unbound form . We introduce a methodology to monitor these changes and to study which mechanisms arrange conformational shifts between the liganded and free forms . Our method is simple , yet it efficiently characterizes the response of pro...
[ "Abstract", "Introduction", "Discussion" ]
[ "physics/condensed", "matter", "biophysics/theory", "and", "simulation", "biophysics/biomacromolecule-ligand", "interactions", "mathematics/statistics", "computational", "biology/molecular", "dynamics", "physics/interdisciplinary", "physics" ]
2009
Perturbation-Response Scanning Reveals Ligand Entry-Exit Mechanisms of Ferric Binding Protein
Ferroportin ( FPN ) is the only known cellular iron exporter in mammalian cells and plays a critical role in the maintenance of both cellular and systemic iron balance . During iron deprivation , the translation of FPN is repressed by iron regulatory proteins ( IRPs ) , which bind to the 5′ untranslated region ( UTR ) ...
Cellular iron homeostasis is maintained by a sophisticated system that responds to iron levels and coordinates the expression of targets important for balancing iron export and uptake with intracellular storage and utilization . Ferroportin is the only known cellular iron exporter in mammalian cells and plays a critica...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cellular", "stress", "responses", "molecular", "cell", "biology", "cell", "biology", "gene", "regulation", "genetics", "molecular", "genetics", "biology", "genetics", "and", "genomics" ]
2013
Iron-Responsive miR-485-3p Regulates Cellular Iron Homeostasis by Targeting Ferroportin
The epidermis is the largest organ of the body for most animals , and the first line of defense against invading pathogens . A breach in the epidermal cell layer triggers a variety of localized responses that in favorable circumstances result in the repair of the wound . Many cellular and genetic responses must be limi...
An epidermal wound provides signals that initiate a variety of localized responses , some of which act to regenerate and repair the breach in the epidermal barrier . The Drosophila melanogaster embryonic epidermis provides an excellent system to discover new genes that regulate wound-healing processes . Using fluoresce...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "model", "organisms", "genetics", "biology", "molecular", "cell", "biology", "genetics", "and", "genomics" ]
2011
Duox, Flotillin-2, and Src42A Are Required to Activate or Delimit the Spread of the Transcriptional Response to Epidermal Wounds in Drosophila
Malaria in pregnancy remains a substantial public health problem in malaria-endemic areas with detrimental outcomes for both the mother and the foetus . The placental changes that lead to some of these detrimental outcomes have been studied , but the mechanisms that lead to these changes are still not fully elucidated ...
Plasmodium infections during pregnancy represent a substantial health burden for mothers and their neonates leading to underweight babies , preterm deliveries , abortions , stillbirths , and even maternal mortality . The placenta , in its role as a bridge between mother and foetus , will either suffer or mediate the in...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta
The mosquito resistance to the insecticides threatens malaria control efforts , potentially becoming a major public health issue . Alternative methods like ivermectin ( IVM ) administration to humans has been suggested as a possible vector control to reduce Plasmodium transmission . Anopheles aquasalis and Anopheles da...
Malaria is one of the most important infectious diseases in the world with hundreds of millions of new cases every year . The disease is caused by parasites of the genus Plasmodium where Plasmodium vivax represent most of the cases in the Americas . Current strategies to combat malaria transmission are being implemente...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "death", "rates", "invertebrates", "parasite", "groups", "medicine", "and", "health", "sciences", "body", "fluids", "oocysts", "plasmodium", "tropical", "diseases", "parasitic", "diseases", "animals", "parasitology", "apicomplexa", "pharmacology", "population", "biology",...
2018
Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi
The sphingolipid ceramide elicits several stress responses , however , organisms survive despite increased ceramide but how they do so is poorly understood . We demonstrate here that the AKT/FOXO pathway regulates survival in increased ceramide environment by metabolic adaptation involving changes in glycolysis and lip...
Ceramide belongs to an important class of lipids called sphingolipids . An increase in ceramide levels elicits several stress responses . We have used Drosophila mutated in ceramide kinase , an enzyme involved in metabolizing ceramide to understand how an organism adapts to stress imposed by ceramide . We find that in ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2013
Survival Response to Increased Ceramide Involves Metabolic Adaptation through Novel Regulators of Glycolysis and Lipolysis
The genomes of all organisms throughout the tree of life are compacted and organized in chromatin by association of chromatin proteins . Eukaryotic genomes encode histones , which are assembled on the genome into octamers , yielding nucleosomes . Post-translational modifications of the histones , which occur mostly on ...
Both Archaea and eukaryotes express histones , but whereas the tertiary structure of histones is conserved , the quaternary structure of histone–DNA complexes is very different . In a recent study , the crystal structure of the archaeal hypernucleosome was revealed to be an “endless” core of interacting histones that w...
[ "Abstract", "Introduction", "Histones", "are", "found", "in", "some", "newly", "discovered", "Archaea", "Conclusion", "Methods" ]
[ "chemical", "compounds", "dna-binding", "proteins", "microbiology", "organic", "compounds", "review", "archaea", "basic", "amino", "acids", "amino", "acids", "epigenetics", "chromatin", "chromosome", "biology", "proteins", "gene", "expression", "chemistry", "histones", ...
2018
Structure and function of archaeal histones
Infection by Listeria monocytogenes ( Lm ) causes serious sepsis and meningitis leading to mortality in neonates . This work explored the ability of CD11chigh lineage DCs to induce CD8+ T-cell immune protection against Lm in mice before 7 days of life , a period symbolized by the absence of murine IL-12p70-producing CD...
Lm is a gram-positive food-borne pathogen that is the ethiological agent of listeriosis , a worldwide disease reported most frequently in developed countries . It can cause spontaneous septic abortions , fatal meningitis or encephalitis in immunocompromised and pregnant individuals . The murine model of systemic Lm inf...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "flow", "cytometry", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "cytokines", "spleen", "immunology", "developmental", "biology", "molecular", "development", "cytotoxic", "t", "cel...
2016
IL-12p40/IL-10 Producing preCD8α/Clec9A+ Dendritic Cells Are Induced in Neonates upon Listeria monocytogenes Infection
MicroRNAs ( miRNAs ) suppress the transcriptional and post-transcriptional expression of genes in plants . Several miRNA families target genes encoding nucleotide-binding site–leucine-rich repeat ( NB-LRR ) plant innate immune receptors . The fungus Fusarium oxysporum f . sp . lycopersici causes vascular wilt disease i...
Fusarium oxysporum is a fungal pathogen that represents a species complex , with members that infect numerous crops . In spite of its importance to agriculture , very little is known about roles of small RNAs in plant immunity against F . oxysporum . In this study , we set up a screen for tomato microRNAs ( miRNAs ) th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "rna", "micrornas", "plant", "science", "plant", "disease", "resistance", "gene", "expression", "genetics", "gene", "regulation", "biology", "and", "life", "sciences", "plant", "pathology", "plant", "pathogens" ]
2014
MicroRNAs Suppress NB Domain Genes in Tomato That Confer Resistance to Fusarium oxysporum
Family studies of individual tissues have shown that gene expression traits are genetically heritable . Here , we investigate cis and trans components of heritability both within and across tissues by applying variance-components methods to 722 Icelanders from family cohorts , using identity-by-descent ( IBD ) estimate...
An important goal in biology is to understand how genotype affects gene expression . Because gene expression varies across tissues , the relationship between genotype and gene expression may be tissue-specific . In this study , we used heritability approaches to study the regulation of gene expression in two tissue typ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "genetics", "and", "genomics/gene", "expression", "genetics", "and", "genomics/complex", "traits", "genetics", "and", "genomics/genetics", "of", "disease", "genetics", "and", "genomics/epigenetics", "genetics", "and", "genomics", "genetics", "and", "genomics/population", ...
2011
Single-Tissue and Cross-Tissue Heritability of Gene Expression Via Identity-by-Descent in Related or Unrelated Individuals
In Europe , the Neolithic transition ( 8 , 000–4 , 000 b . c . ) from hunting and gathering to agricultural communities was one of the most important demographic events since the initial peopling of Europe by anatomically modern humans in the Upper Paleolithic ( 40 , 000 b . c . ) . However , the nature and speed of th...
The transition from a hunter–gatherer existence to a sedentary farming-based lifestyle has had key consequences for human groups around the world and has profoundly shaped human societies . Originating in the Near East around 11 , 000 y ago , an agricultural lifestyle subsequently spread across Europe during the New St...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology", "evolutionary", "biology/human", "evolution", "genetics", "and", "genomics/population", "genetics" ]
2010
Ancient DNA from European Early Neolithic Farmers Reveals Their Near Eastern Affinities
Betanodaviruses cause massive mortality in marine fish species with viral nervous necrosis . The structure of a T = 3 Grouper nervous necrosis virus-like particle ( GNNV-LP ) is determined by the ab initio method with non-crystallographic symmetry averaging at 3 . 6 Å resolution . Each capsid protein ( CP ) shows three...
Betanodaviruses belong to the family Nodaviridae and cause the mortality of numerous larval-stage fish species . Here we report protein crystal structures of a piscine betanodavirus , the Grouper nervous necrosis virus ( GNNV ) , in four different forms . Highlights are two structural features that contribute to the vi...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Crystal Structures of a Piscine Betanodavirus: Mechanisms of Capsid Assembly and Viral Infection
Clathrin-mediated endocytosis ( CME ) and activity-dependent bulk endocytosis ( ADBE ) are two predominant forms of synaptic vesicle ( SV ) endocytosis , elicited by moderate and strong stimuli , respectively . They are tightly coupled with exocytosis for sustained neurotransmission . However , the underlying mechanism...
The arrival of an action potential at the nerve end induces synaptic vesicle ( SV ) exocytosis to allow the release of chemical neurotransmitters and the rapid transmission of signals . SV endocytosis is in turn elicited in order to rapidly replenish the vesicle pool in neurons . Therefore , tight coupling between exoc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "neuromuscular", "junctions", "nervous", "system", "cell", "processes", "electrophysiology", "pigments", "neuroscience", "surgical", "and", "invasive", "medical", "procedures", "developmental", "biology", "functional", "electrical", ...
2017
A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis
The lack of a mutant phenotype in homozygous mutant individuals’ due to compensatory gene expression triggered upstream of protein function has been identified as genetic compensation . Whilst this intriguing process has been recognized in zebrafish , the presence of homozygous loss of function mutations in healthy hum...
Many healthy individuals carry loss of function mutations in essential genes that would normally be deleterious for survival . Intriguingly , it may be the presence of the genomic lesion itself in these individuals that triggers the compensatory pathways . It is not known how widespread this phenomenon is in vertebrate...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "fish", "medicine", "and", "health", "sciences", "morpholino", "rna", "interference", "skeletal", "muscles", "messenger", "rna", "vertebrates", "nucleotides", "animals", "animal", "models", "osteichthyes", "developmental", "biology", "model", "organisms", "untranslated", ...
2018
Genetic compensation triggered by actin mutation prevents the muscle damage caused by loss of actin protein
Currently , our knowledge of how pathogenic fungi grow in mammalian host environments is limited . Using a chemotherapeutic murine model of invasive pulmonary aspergillosis ( IPA ) and 1H-NMR metabolomics , we detected ethanol in the lungs of mice infected with Aspergillus fumigatus . This result suggests that A . fumi...
Metabolic flexibility is important for human pathogens like Aspergillus fumigatus as it allows adaptation to dynamic infection induced microenvironments . Consequently , identification of fungal metabolic pathways critical for in vivo growth may uncover novel virulence mechanisms and new therapeutic opportunities . To ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "mycology", "biology", "microbiology", "host-pathogen", "interaction", "pathogenesis" ]
2011
In vivo Hypoxia and a Fungal Alcohol Dehydrogenase Influence the Pathogenesis of Invasive Pulmonary Aspergillosis
It is generally accepted that neuroendocrine cells regulate dense core vesicle ( DCV ) biogenesis and cargo packaging in response to secretory demands , although the molecular mechanisms of this process are poorly understood . One factor that has previously been implicated in DCV regulation is IA-2 , a catalytically in...
Within secretory cells , hormones are packaged into vesicles ( called DCVs ) that are released upon stimulation . The number of DCVs is regulated to meet the secretory demands of the cell by a mechanism that is poorly understood , although a protein in the membrane of DCVs , called IA-2 , is thought to play a role . A ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics", "developmental", "biology", "diabetes", "and", "endocrinology", "cell", "biology/neuronal", "signaling", "mechanisms" ]
2009
Loss of the Transcriptional Repressor PAG-3/Gfi-1 Results in Enhanced Neurosecretion that is Dependent on the Dense-Core Vesicle Membrane Protein IDA-1/IA-2
Japanese encephalitis ( JE ) is a global public health issue that has spread widely to more than 20 countries in Asia and has extended its geographic range to the south Pacific region including Australia . JE has become the most important cause of viral encephalitis in the world . Japanese encephalitis viruses ( JEV ) ...
Japanese encephalitis virus ( JEV ) is a mosquito-borne virus that causes Japanese encephalitis ( JE ) with significant morbidity and mortality . Five genotypes ( genotype I–V ) have been identified based on the nucleotide sequence of viral envelope ( E ) gene of JEV . To date , the only known strain of genotype V is M...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "virology", "emerging", "viral", "diseases", "biology", "microbiology" ]
2011
Genotype V Japanese Encephalitis Virus Is Emerging
The interplay between excitatory and inhibitory neurons imparts rich functions of the brain . To understand the synaptic mechanisms underlying neuronal computations , a fundamental approach is to study the dynamics of excitatory and inhibitory synaptic inputs of each neuron . The traditional method of determining input...
To understand synaptic mechanisms underlying neuronal computations , a fundamental approach is to use voltage clamp to measure the dynamics of excitatory and inhibitory input conductances . Due to the space clamp effect , the measured conductance in general deviates from the local input conductance on the dendrites , h...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "nervous", "system", "membrane", "potential", "electrophysiology", "neuroscience", "simulation", "and", "modeling", "bioassays", "and", "physiological", "analysis", "neuronal", "dendrites", "research", "and", "analysis", "methods",...
2019
Determination of effective synaptic conductances using somatic voltage clamp
Blood flukes of the genus Schistosoma cause schistosomiasis—a neglected tropical disease ( NTD ) that affects more than 200 million people worldwide . Studies of schistosome genomes have improved our understanding of the molecular biology of flatworms , but most of them have focused largely on protein-coding genes . Sm...
Human schistosomiasis is a chronic , neglected tropical disease ( NTD ) that is predominantly caused by the blood flukes Schistosoma haematobium , S . mansoni and S . japonicum . Infections by S . haematobium and/or S . mansoni are highly prevalent in Africa , affecting ~ 200 million people . The decoding of schistosom...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Conclusions" ]
[ "sequencing", "techniques", "schistosoma", "invertebrates", "schistosoma", "mansoni", "helminths", "gene", "regulation", "animals", "invertebrate", "genomics", "micrornas", "genome", "analysis", "molecular", "biology", "techniques", "rna", "sequencing", "research", "and", ...
2018
The small RNA complement of adult Schistosoma haematobium
During cell division , segregation of sister chromatids to daughter cells is achieved by the poleward pulling force of microtubules , which attach to the chromatids by means of a multiprotein complex , the kinetochore . Kinetochores assemble at the centromeric DNA organized by specialized centromeric nucleosomes . In c...
During cell division , replicated DNA molecules are pulled to daughter cells by microtubules , which originate at the spindle poles and attach to a multiprotein complex , the kinetochore . The kinetochore assembles at a special region of the chromosome , termed the centromere . The kinetochore is comprised of more than...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology", "chromosome", "biology", "centromeres", "biology", "molecular", "cell", "biology" ]
2012
Histone H3 Localizes to the Centromeric DNA in Budding Yeast
In addition to the DNA contributed by sperm and oocytes , embryos receive parent-specific epigenetic information that can include histone variants , histone post-translational modifications ( PTMs ) , and DNA methylation . However , a global view of how such marks are erased or retained during gamete formation and repr...
Successful reproduction depends upon the receipt and processing of distinct chromatin packages from sperm and oocytes . This includes not only a unique complement of DNA , but information in the form of proteins , such as histones , that differentially package the DNA in each cell type . For example , histone variants ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biochemistry", "developmental", "biology", "model", "organisms", "cell", "biology", "genetics", "biology", "and", "life", "sciences", "proteomics", "molecular", "cell", "biology", "research", "and", "analysis", "methods" ]
2014
The Specification and Global Reprogramming of Histone Epigenetic Marks during Gamete Formation and Early Embryo Development in C. elegans
Flexible or innovative behavior is advantageous , especially when animals are exposed to frequent and unpredictable environmental perturbations . Improved cognitive abilities can help animals to respond quickly and adequately to environmental dynamics , and therefore changing environments may select for higher cognitiv...
Animals with higher cognitive abilities should be better capable of producing new , modified , or innovative behaviors as this ability could allow them to cope better with unpredictable environmental changes . Changing environments may hence select for higher cognitive abilities . Similarly , changing conditions during...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology/animal", "behavior" ]
2010
Environmental Change Enhances Cognitive Abilities in Fish
Numerous biomolecular interactions involve unstructured protein regions , but how to exploit such interactions to enhance the affinity of a lead molecule in the context of rational drug design remains uncertain . Here clarification was sought for cases where interactions of different ligands with the same disordered pr...
Life as we know it depends on interactions between proteins . There is substantial evidence that many interactions between proteins involve very flexible protein regions . These disordered regions may undergo disorder/order transitions upon forming an interaction with another protein . Many successful approaches to med...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Elucidation of Ligand-Dependent Modulation of Disorder-Order Transitions in the Oncoprotein MDM2
Centromeres are specialized chromatin regions marked by the presence of nucleosomes containing the centromere-specific histone H3 variant CENP-A , which is essential for chromosome segregation . Assembly and disassembly of nucleosomes is intimately linked to DNA topology , and DNA topoisomerases have previously been im...
Centromeres are unique regions on eukaryotic chromosomes that are essential for chromosome segregation at mitosis and meiosis . Centromere identity and function depends on the presence of specialized chromatin with nucleosomes containing the centromere-specific histone H3 variant CENP-A . Assembly and disassembly of nu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "epigenetics", "biology", "chromatin", "genetics", "and", "genomics" ]
2013
DNA Topoisomerase III Localizes to Centromeres and Affects Centromeric CENP-A Levels in Fission Yeast
Efficient infection control requires potent T-cell responses at sites of pathogen replication . However , the regulation of T-cell effector function in situ remains poorly understood . Here , we show key differences in the regulation of effector activity between CD4+ and CD8+ T-cells during skin infection with HSV-1 . ...
HSV-1 is a widely distributed pathogen causing a life-long latent infection associated with periodic bouts of reactivation and severe clinical complications . Adaptive immune responses encompassing CD4+ and CD8+ T-cell activities are key to both the clearance of infectious virus and the control of latent infection . Ho...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "dermatology", "medicine", "and", "health", "sciences", "immune", "cells", "herpes", "simplex", "immune", "activation", "antigen-presenting", "cells", "immunology", "microbiology", "infectious", "disease", "immunology", "skin", "infections", "sexually", ...
2014
Distinct APC Subtypes Drive Spatially Segregated CD4+ and CD8+ T-Cell Effector Activity during Skin Infection with HSV-1
Planar cell polarity ( PCP ) signaling controls the global orientation of surface structures , such as hairs and bristles , in both vertebrates and invertebrates . In Frizzled6-/- ( Fz6-/- ) mice , hair follicle orientations on the head and back are nearly random at birth , but reorient during early postnatal developme...
Hair , feather , and scale patterns are a universal feature of vertebrate surface morphology . These patterns are under precise genetic control as seen by their species-specificity and by their alterations in different breeds of domesticated animals . The first clues to the mechanism of hair patterning in mammals came ...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Identification of Astrotactin2 as a Genetic Modifier That Regulates the Global Orientation of Mammalian Hair Follicles
We have developed a new approach to characterize allele-specific timing of DNA replication genome-wide in human primary basophilic erythroblasts . We show that the two chromosome homologs replicate at the same time in about 88% of the genome and that large structural variants are preferentially associated with asynchro...
DNA replication in mammalian cells proceeds according to a distinct order . Genes that are expressed tend to replicate before genes that are not expressed . We report here that we have developed a method to measure the timing of replication of the maternal and paternal chromosomes separately . We found that the paterna...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "functional", "genomics", "cell", "biology", "chromosome", "biology", "genomic", "imprinting", "gene", "expression", "genetics", "gene", "regulation", "biology", "and", "life", "sciences", "epigenetics", "molecular", "genetics", "genomics", "...
2014
Allele-Specific Genome-wide Profiling in Human Primary Erythroblasts Reveal Replication Program Organization
Currently , malaria rapid diagnostic tests ( RDTs ) are widely used for malaria diagnosis , but test performance and the factors that lead to failure of Plasmodium ovale detection are not well understood . In this study , three pLDH-based RDTs were evaluated using cases in China that originated in Africa . The sensitiv...
Plasmodium ovale ( P . ovale ) are under-estimated and overshadowed by other malaria parasites in tropical countries , which can cause chronic infections that last from months to years . The chronic infection caused by P . ovale should be of concern in the context of the long-term goal of eliminating malaria . Rapid di...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "parasite", "groups", "medicine", "and", "health", "sciences", "body", "fluids", "respiratory", "infections", "plasmodium", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "pulmonology", "parasitology", "parasitemia", "apicomplexa", "protozoans",...
2019
Assessment of false negative rates of lactate dehydrogenase-based malaria rapid diagnostic tests for Plasmodium ovale detection
Clostridium difficile is a Gram-positive , spore-forming anaerobic bacterium that infects the colon , causing symptoms ranging from infectious diarrhea to fulminant colitis . In the last decade , the number of C . difficile infections has dramatically risen , making it the leading cause of reported hospital acquired in...
Clostridium difficile is a pathogenic bacterium that infects the colon and is the leading cause of infectious diarrhea in the United States . C . difficile mediated disease is driven by the production of two toxins , TcdA and TcdB . The toxins cause severe damage to the intestinal epithelial layer of the colon resultin...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "heme", "medicine", "and", "health", "sciences", "gut", "bacteria", "pathology", "and", "laboratory", "medicine", "animal", "models", "of", "disease", "microbiology", "operons", "toxicology", "animal", "models", "toxicity", "model", "organisms", "gastroenterology", "a...
2018
Heme sensing and detoxification by HatRT contributes to pathogenesis during Clostridium difficile infection
Ocular dominance plasticity is a well-documented phenomenon allowing us to study properties of cortical maturation . Understanding this maturation might be an important step towards unravelling how cortical circuits function . However , it is still not fully understood which mechanisms are responsible for the opening a...
During the development of the brain , visual cortex has a period of increased plasticity . Closing one eye for multiple days during this period can have a profound and life-long impact on neuronal responses . A well-established hypothesis is that the absolute level of inhibition regulates this period . In light of rece...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "neural", "networks", "nervous", "system", "social", "sciences", "electrophysiology", "neuroscience", "synaptic", "plasticity", "vision", "eyes", "neuronal", "plasticity", "mood", "disorders", "developmental", "neuroscience", "comp...
2019
Synaptic plasticity onto inhibitory neurons as a mechanism for ocular dominance plasticity
Murray Valley encephalitis virus ( MVEV ) is the most serious of the endemic arboviruses in Australia . It was responsible for six known large outbreaks of encephalitis in south-eastern Australia in the 1900s , with the last comprising 58 cases in 1974 . Since then MVEV clinical cases have been largely confined to the ...
An outbreak of Murray Valley encephalitis with 17 confirmed cases occurred across Australia in 2011 . This outbreak involved parts of Australia where cases had not occurred for many decades . The epidemiology in this outbreak reflects a change that has occurred over the past 15 years , with more non-Aboriginal cases , ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "mosquitoes", "zoonoses", "disease", "ecology", "infectious", "disease", "epidemiology", "epidemiology", "neglected", "tropical", "diseases", "arboviral", "infections", "vectors", "and", "hosts", "public", "health" ]
2014
The Changing Epidemiology of Murray Valley Encephalitis in Australia: The 2011 Outbreak and a Review of the Literature
Comparison of elastic network model predictions with experimental data has provided important insights on the dominant role of the network of inter-residue contacts in defining the global dynamics of proteins . Most of these studies have focused on interpreting the mean-square fluctuations of residues , or deriving the...
As more protein structures are solved , we are able to perform a more critical assessment of the relationship between protein structure and dynamics , and to gain a deeper understanding of the major determinants of structural dynamics . Here we perform a systematic study on a set of proteins structurally determined by ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/macromolecular", "structure", "analysis", "biophysics/theory", "and", "simulation", "computational", "biology" ]
2010
Using Entropy Maximization to Understand the Determinants of Structural Dynamics beyond Native Contact Topology
Modulation is essential for adjusting neurons to prevailing conditions and differing demands . Yet understanding how modulators adjust neuronal properties to alter information processing remains unclear , as is the impact of neuromodulation on energy consumption . Here we combine two computational models , one Hodgkin-...
The properties of neurons and neural circuits can be adjusted by neuromodulators , molecules that alter their ability to respond to future activity . Many neuromodulators target voltage-dependent ion channels , molecular components of cell membranes that influence the electrical activity of neurons . Because of their i...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "neurochemistry", "engineering", "and", "technology", "signal", "processing", "membrane", "potential", "social", "sciences", "light", "electrophysiology", "neuroscience", "animals", "electromagnetic", "radiation", "a...
2018
Modulation of voltage-dependent K+ conductances in photoreceptors trades off investment in contrast gain for bandwidth
Numerous studies are currently underway to characterize the microbial communities inhabiting our world . These studies aim to dramatically expand our understanding of the microbial biosphere and , more importantly , hope to reveal the secrets of the complex symbiotic relationship between us and our commensal bacterial ...
The emerging field of metagenomics aims to understand the structure and function of microbial communities solely through DNA analysis . Current metagenomics studies comparing communities resemble large-scale clinical trials with multiple subjects from two general populations ( e . g . sick and healthy ) . To improve an...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "computational", "biology/metagenomics" ]
2009
Statistical Methods for Detecting Differentially Abundant Features in Clinical Metagenomic Samples
Loss-of-function mutations in PINK1 and Parkin cause parkinsonism in humans and mitochondrial dysfunction in model organisms . Parkin is selectively recruited from the cytosol to damaged mitochondria to trigger their autophagy . How Parkin recognizes damaged mitochondria , however , is unknown . Here , we show that exp...
Mutations in the PINK1 or Parkin genes lead to an inherited form of Parkinson disease . Understanding how the products of these genes work may give us insights into what goes wrong in these patients and in Parkinson disease more generally . Previous studies in flies and mice , and in human cells suggest that PINK1 and ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "cell", "biology/cell", "signaling", "cell", "biology", "neurological", "disorders/movement", "disorders", "neuroscience/neurobiology", "of", "disease", "and", "regeneration" ]
2010
PINK1 Is Selectively Stabilized on Impaired Mitochondria to Activate Parkin
Cutaneous Leishmania major has affected many travelers including military personnel in Iraq and Afghanistan . Optimal treatment for this localized infection has not been defined , but interestingly the parasite is thermosensitive . Participants with parasitologically confirmed L . major infection were randomized to rec...
Cutaneous leishmaniasis , a parasitic skin infection transmitted by the bite of a sand fly , can result in chronic skin sores and is estimated to affect more than 1 . 5 million persons worldwide . While the infection generally heals on its own in months to years , treatment can be expensive and difficult . We compared ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/neglected", "tropical", "diseases", "infectious", "diseases/tropical", "and", "travel-associated", "diseases" ]
2010
A Randomized Controlled Trial of Local Heat Therapy Versus Intravenous Sodium Stibogluconate for the Treatment of Cutaneous Leishmania major Infection
Digestion of blood in the midgut of Aedes aegypti results in the release of pro-oxidant molecules that can be toxic to the mosquito . We hypothesized that after a blood meal , the antioxidant capacity of the midgut is increased to protect cells against oxidative stress . Concomitantly , pathogens present in the blood i...
Mosquitoes ingest large amounts of blood , a rich and abundant source of energy to sustain egg production . Blood digestion offers challenges to the insect , like managing high concentrations of heme and iron , pro-oxidant and potentially toxic molecules derived from hemoglobin . Mosquitoes and other blood-feeding arth...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "body", "fluids", "rna", "interference", "pathology", "and", "laboratory", "medicine", "enzymes", "pathogens", "enzymology", "microbiology", "animals", "viruses", "rna", "viruses", "epigenetics", "insect", "vecto...
2017
Catalase protects Aedes aegypti from oxidative stress and increases midgut infection prevalence of Dengue but not Zika
Licensed human papillomavirus ( HPV ) vaccines provide near complete protection against the types of HPV that most commonly cause anogenital and oropharyngeal cancers ( HPV 16 and 18 ) when administered to individuals naive to these types . These vaccines , like most other prophylactic vaccines , appear to protect by g...
There is an urgent need to better understand how to reliably generate effective vaccines , particularly subunit vaccines , as certain pathogens are considered to pose too great of a safety risk to be developed as live , attenuated or killed vaccines ( e . g . , HIV-1 ) . The human papillomavirus ( HPV ) vaccines are tw...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "humoral", "immunity", "medicine", "and", "health", "sciences", "immune", "cells", "immunology", "vaccines", "infectious", "disease", "immunology", "sexually", "transmitted", "diseases", "class", "switching", "vaccination", "and", "immunization", "anti...
2014
Characteristics of Memory B Cells Elicited by a Highly Efficacious HPV Vaccine in Subjects with No Pre-existing Immunity
The snake Bothrops atrox is responsible for the majority of envenomings in the northern region of South America . Severe local effects , including hemorrhage , which are mainly caused by snake venom metalloproteinases ( SVMPs ) , are not fully neutralized by conventional serum therapy . Little is known about the immuno...
In this work , we propose a new screening strategy to produce monoclonal antibodies against Atr-I , a P-I class SVMP from Bothrops atrox , which is the snake responsible for the majority of the accidents in South America . SVMPs are the main toxic factors in Bothrops venom causing systemic and local hemorrhage , which ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "humoral", "immunity", "biochemistry", "immunochemistry", "clinical", "immunology", "immunity", "biology", "and", "life", "sciences", "immunology", "vaccination", "and", "immunization", "immunotherapy" ]
2014
Use of a Synthetic Biosensor for Neutralizing Activity-Biased Selection of Monoclonal Antibodies against Atroxlysin-I, an Hemorrhagic Metalloproteinase from Bothrops atrox Snake Venom
Prolific sheep have proven to be a valuable model to identify genes and mutations implicated in female fertility . In the Lacaune sheep breed , large variation in litter size is genetically determined by the segregation of a fecundity major gene influencing ovulation rate , named FecL and its prolific allele FecLL . Ou...
Prolific sheep have proven to be a valuable model to identify genes and mutations implicated in ovarian function and female fertility . Indeed , fecundity genes of the Bone Morphogenetic Protein ( BMP ) family discovered in sheep was evidenced as genetic candidates to explain female infertility pathologies . Studying F...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
The Highly Prolific Phenotype of Lacaune Sheep Is Associated with an Ectopic Expression of the B4GALNT2 Gene within the Ovary
Dengue vaccines will soon provide a new tool for reducing dengue disease , but the effectiveness of widespread vaccination campaigns has not yet been determined . We developed an agent-based dengue model representing movement of and transmission dynamics among people and mosquitoes in Yucatán , Mexico , and simulated v...
Dengue is a mosquito-transmitted viral disease that is common throughout the tropics . Despite a long history in humans and extensive efforts to control dengue transmission in many countries , the number , severity , and geographic range of reported cases is increasing . Most control efforts have focused on controlling...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "infectious", "disease", "epidemiology", "immunology", "geographical", "locations", "animals", "vaccines", "preventive", "medicine", "north", "america", "infectious", "disease", "control", "vaccination", "and", "im...
2016
Projected Impact of Dengue Vaccination in Yucatán, Mexico
Global warming and ocean acidification are forecast to exert significant impacts on marine ecosystems worldwide . However , most of these projections are based on ecological proxies or experiments on single species or simplified food webs . How energy fluxes are likely to change in marine food webs in response to futur...
Healthy marine ecosystems are crucial for people’s livelihoods and food production . Global climate stressors , such as warming and ocean acidification , can drastically impact the structure and function of marine food webs , diminishing the production of goods and services . Our ability to predict how future food webs...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "energy", "flow", "ecology", "and", "environmental", "sciences", "marine", "ecosystems", "marine", "biology", "biomass", "(ecology)", "plant", "science", "herbivory", "plant", "ecology", "research", "facilities", "research", "and", "analysis", "methods", "ecological", ...
2018
Climate change could drive marine food web collapse through altered trophic flows and cyanobacterial proliferation
Mycobacterium tuberculosis heparin-binding hemagglutinin ( HBHA ) , a virulence factor involved in extrapulmonary dissemination and a strong diagnostic antigen against tuberculosis , is both surface-associated and secreted . The role of HBHA in macrophages during M . tuberculosis infection , however , is less well know...
Cell death is a common outcome during infection with a number of pathogenic microorganisms . Therefore , defining the factors responsible for killing of host cells is important to uncovering mechanisms of pathogenesis . World-wide , two billon people are latently infected with Mycobacterium tuberculosis , which is stil...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "bacterial", "diseases", "infectious", "diseases", "mycobacterium", "immunity", "innate", "immunity", "microbial", "pathogens", "biology", "microbiology" ]
2011
Targeting of Mycobacterium tuberculosis Heparin-Binding Hemagglutinin to Mitochondria in Macrophages
Many studies have presented virus sequences which suggest the existence of a variety of putative new phleboviruses transmitted by sandflies in the Old World . However , in most of these studies , only partial sequences in the polymerase or the nucleoprotein genes were characterised . Therefore to further our understand...
We provide evidence that sandfly-borne phleboviruses belonging to 3 distinct genetic and phylogenetic groups ( Sandfly fever Naples virus [SFNV] , Sandfly fever Sicilian virus [SFSV] , and Salehabad virus [SALV] ) co-circulate in Adana city , in Mediterranean Turkey . While Adana virus was recently described as a new m...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "turkey", "(country)", "sequencing", "techniques", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "population", "genetics", "geographical", "locations", "microbiology", "sand", "flies", "gene", "pool", "viruses", "sig...
2016
Sandfly-Borne Phlebovirus Isolations from Turkey: New Insight into the Sandfly fever Sicilian and Sandfly fever Naples Species
Agents living in volatile environments must be able to detect changes in contingencies while refraining to adapt to unexpected events that are caused by noise . In Reinforcement Learning ( RL ) frameworks , this requires learning rates that adapt to past reliability of the model . The observation that behavioural flexi...
In stable contexts , animals and humans exhibit automatic behaviour that allows them to make fast decisions . However , these automatic processes exhibit a lack of flexibility when environmental contingencies change . In the present paper , we propose a model of behavioural automatization that is based on adaptive forg...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2019
Learning and forgetting using reinforced Bayesian change detection
This paper presents the development of an agent-based model ( ABM ) to incorporate climatic drivers which affect tsetse fly ( G . m . morsitans ) population dynamics , and ultimately disease transmission . The model was used to gain a greater understanding of how tsetse populations fluctuate seasonally , and investigat...
African trypanosomiasis is a parasitic disease which affects humans and other animals in 36 sub-Saharan African countries . The disease is transmitted by the tsetse fly , and the human form of the diseases is known as sleeping sickness . In an attempt to improve our understanding of the mechanisms which contribute to s...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "death", "rates", "invertebrates", "medicine", "and", "health", "sciences", "ruminants", "african", "trypanosomiasis", "tropical", "diseases", "vertebrates", "parasitic", "diseases", "animals", "mammals", "parasitic", "protozoans", "glossina", "simulation", "and", "modeli...
2018
An agent-based model of tsetse fly response to seasonal climatic drivers: Assessing the impact on sleeping sickness transmission rates
What is the mechanism through which transcription factors ( TFs ) assemble specifically along the enhancer DNA ? The IFN-β enhanceosome provides a good model system: it is small; its components' crystal structures are available; and there are biochemical and cellular data . In the IFN-β enhanceosome , there are few pro...
An enhanceosome is a functional unit that consists of DNA segment called enhancer; its transcription factors ( TFs ) ; and their interacting cofactors . To function , the TFs must assemble on their corresponding response elements ( REs ) cooperatively . Understanding how TFs assemble is important because the TF combina...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2011
The Role of Response Elements Organization in Transcription Factor Selectivity: The IFN-β Enhanceosome Example
Family with sequence similarity 20 , -member C ( FAM20C ) is highly expressed in the mineralized tissues of mammals . Genetic studies showed that the loss-of-function mutations in FAM20C were associated with human lethal osteosclerotic bone dysplasia ( Raine Syndrome ) , implying an inhibitory role of this molecule in ...
A recent study demonstrated that the inactivating mutations in the FAM20C gene were associated with lethal osteosclerotic bone dysplasia characterized by a generalized hardening of all bones; this observation implied an inhibitory role of FAM20C during bone formation . However , in vitro studies revealed a contradictor...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "musculoskeletal", "system", "bone", "physiology", "genetics", "biology", "anatomy", "and", "physiology", "genetics", "and", "genomics" ]
2012
Inactivation of a Novel FGF23 Regulator, FAM20C, Leads to Hypophosphatemic Rickets in Mice
Infectious disease is a leading threat to public health , economic stability , and other key social structures . Efforts to mitigate these impacts depend on accurate and timely monitoring to measure the risk and progress of disease . Traditional , biologically-focused monitoring techniques are accurate but costly and s...
Even in developed countries , infectious disease has significant impact; for example , flu seasons in the United States take between 3 , 000 and 49 , 000 lives . Disease surveillance , traditionally based on patient visits to health providers and laboratory tests , can reduce these impacts . Motivated by cost and timel...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "public", "and", "occupational", "health", "infectious", "diseases", "computer", "and", "information", "sciences", "data", "management", "ecology", "and", "environmental", "sciences", "computer", "modeling", "nature-society", "interactions", "medicine", "and", "health", ...
2014
Global Disease Monitoring and Forecasting with Wikipedia
Face cleanliness is a core component of the SAFE ( Surgery , Antibiotics , Facial cleanliness , and Environmental improvements ) strategy for trachoma control . Understanding knowledge , attitudes , and behaviors related to face washing may be helpful for designing effective interventions for improving facial cleanline...
Facial cleanliness is a core component of the SAFE ( Surgery , Antibiotics , Facial cleanliness , and Environmental improvements ) strategy for trachoma control . We conducted a mixed methods study in a trachoma hyperendemic region of rural Ethiopia to better understand knowledge , attitudes , and behaviors related to ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "face", "chemical", "compounds", "engineering", "and", "technology", "tropical", "diseases", "salts", "geographical", "locations", "cell", "phones", "bacterial", "diseases", "eye", "diseases", "neglected", "tropical", "diseases",...
2016
‘If an Eye Is Washed Properly, It Means It Would See Clearly’: A Mixed Methods Study of Face Washing Knowledge, Attitudes, and Behaviors in Rural Ethiopia
Somatic copy number variations ( CNVs ) play a crucial role in development of many human cancers . The broad availability of next-generation sequencing data has enabled the development of algorithms to computationally infer CNV profiles from a variety of data types including exome and targeted sequence data; currently ...
We present Bamgineer , a software program to introduce user-defined , haplotype-specific copy number variants ( CNVs ) at any frequency into standard Binary Alignment Mapping ( BAM ) files . Copy number gains are simulated by introducing new DNA sequencing read pairs sampled from existing reads and modified to contain ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "sequencing", "techniques", "alleles", "genetic", "mapping", "genome", "analysis", "copy", "number", "variation", "molecular", "genetics", "molecular", "biology", "techniques", "research", "and", "analysis", "methods", "sequence", "analysis", "genome", "complexity", "se...
2018
Bamgineer: Introduction of simulated allele-specific copy number variants into exome and targeted sequence data sets
Andes virus ( ANDV ) is a human-pathogenic hantavirus . Hantaviruses presumably initiate their mRNA synthesis by using cap structures derived from host cell mRNAs , a mechanism called cap-snatching . A signature for a cap-snatching endonuclease is present in the N terminus of hantavirus L proteins . In this study , we ...
Hantaviruses may cause severe disease in humans , either hemorrhagic fever or cardiopulmonary syndrome . Both conditions are associated with high lethality . Vaccines and effective treatments approved for application in humans are not available . The L protein plays a central role in hantavirus replication and represen...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "crystal", "structure", "pathology", "and", "laboratory", "medicine", "manganese", "pathogens", "condensed", "matter", "physics", "microbiology", "enzymology", "orthomyxoviruses", "viruses", "organisms", "hantavirus", "monomers", "...
2016
Atomic Structure and Biochemical Characterization of an RNA Endonuclease in the N Terminus of Andes Virus L Protein
Elucidating virus-host interactions responsible for HIV-1 transmission is important for advancing HIV-1 prevention strategies . To this end , single genome amplification ( SGA ) and sequencing of HIV-1 within the context of a model of random virus evolution has made possible for the first time an unambiguous identifica...
Understanding the biology of sexual transmission of HIV-1 could contribute importantly to the development of effective prevention measures . However , different routes of virus transmission ( vaginal , rectal , penile or oral ) and inaccessibility of tissues at or near the time of virus transmission make this goal elus...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/virus", "evolution", "and", "symbiosis", "virology/vaccines", "virology/immunodeficiency", "viruses", "virology/animal", "models", "of", "infection", "virology", "infectious", "diseases/hiv", "infection", "and", "aids", "virology/immune", "evasion", "infectious", "d...
2010
High Multiplicity Infection by HIV-1 in Men Who Have Sex with Men