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Motion tracking is a challenge the visual system has to solve by reading out the retinal population . It is still unclear how the information from different neurons can be combined together to estimate the position of an object . Here we recorded a large population of ganglion cells in a dense patch of salamander and g...
It remains unclear how the brain is able to track the location of moving objects by reading the spike trains received from the retina . To address this question , we recorded a large population of ganglion cells , the retinal output , in a dense patch of salamander and guinea pig retinas while displaying a bar moving i...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
High Accuracy Decoding of Dynamical Motion from a Large Retinal Population
Cytotoxic T-lymphocytes play an important role in the protection against viral infections , which they detect through the recognition of virus-derived peptides , presented in the context of MHC class I molecules at the surface of the infected cell . The transporter associated with antigen processing ( TAP ) plays an es...
Herpesviruses have the conspicuous property that they persist for life in the infected host . This is also the case for varicelloviruses , a large subfamily of herpesviruses with representatives in humans ( varicella zoster virus or VZV ) , cattle ( bovine herpesvirus 1 or BHV-1 ) , pigs ( pseudorabies virus or PRV ) ,...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/immunomodulation", "immunology/antigen", "processing", "and", "recognition", "virology/immune", "evasion" ]
2008
Varicellovirus UL49.5 Proteins Differentially Affect the Function of the Transporter Associated with Antigen Processing, TAP
The effect of biodiversity on the ability of parasites to infect their host and cause disease ( i . e . disease risk ) is a major question in pathology , which is central to understand the emergence of infectious diseases , and to develop strategies for their management . Two hypotheses , which can be considered as ext...
Biodiversity has been proposed as a major ecological factor determining disease prevalence . However , the relationship between biodiversity and disease risk remains underexplored . Few studies focus on host-virus systems and , particularly on plant viruses . To address this subject the prevalence of virus infection an...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "ecology", "virology", "biology", "microbiology", "biodiversity", "host-pathogen", "interaction" ]
2012
Effect of Biodiversity Changes in Disease Risk: Exploring Disease Emergence in a Plant-Virus System
The mechanism of circadian oscillations in mammals is cell autonomous and is generated by a set of genes that form a transcriptional autoregulatory feedback loop . While these “clock genes” are well conserved among animals , their specific functions remain to be fully understood and their roles in central versus periph...
Although significant progress has been made in unraveling the molecular mechanism of circadian clocks in mammals , previous work has focused on germline mutations and in vitro methods for analysis . To address the function of clock genes , it is necessary to develop tools to manipulate circadian genes in a conditional ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "biochemistry", "physiology", "mus", "(mouse)", "neuroscience", "genetics", "and", "genomics" ]
2007
Inducible and Reversible Clock Gene Expression in Brain Using the tTA System for the Study of Circadian Behavior
Ace is an adhesin to collagen from Enterococcus faecalis expressed conditionally after growth in serum or in the presence of collagen . Here , we generated an ace deletion mutant and showed that it was significantly attenuated versus wild-type OG1RF in a mixed infection rat endocarditis model ( P<0 . 0001 ) , while no ...
Enterococcus faecalis was recognized as a common cause of infective endocarditis ( IE ) by the early 1900s . It is still third in community-onset IE , but is the second most common cause of hospital-associated IE . Complications due to E . faecalis IE include congestive heart failure , septic emboli and death and curre...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/bacterial", "infections", "microbiology/cellular", "microbiology", "and", "pathogenesis", "microbiology/immunity", "to", "infections" ]
2010
Importance of the Collagen Adhesin Ace in Pathogenesis and Protection against Enterococcus faecalis Experimental Endocarditis
Pharmacologic stimulation of innate immune processes represents an attractive strategy to achieve multiple therapeutic outcomes including inhibition of virus replication , boosting antitumor immunity , and enhancing vaccine immunogenicity . In light of this we sought to identify small molecules capable of activating th...
STING is a pattern recognition receptor of cyclic dinucleotides as well as an innate immune adaptor protein that enables signaling from cytoplasmic receptors to the transcription factor interferon regulatory factor 3 . Initiation of these pathways leads to the expression of type I interferons and proteins associated wi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Characterization of a Novel Human-Specific STING Agonist that Elicits Antiviral Activity Against Emerging Alphaviruses
Highly social insects are characterized by caste dimorphism , with distinct size differences of reproductive organs between fertile queens and the more or less sterile workers . An abundance of nutrition or instruction via diet-specific compounds has been proposed as explanations for the nutrition-driven queen and work...
In honeybees , nutrition drives dimorphic size development of reproductive organs in fertile queens and sterile workers . The first induced morphological mutants in honeybees demonstrate that this developmental plasticity requires a genetic program that is switched “ON” by the feminizer ( fem ) gene .
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "honey", "bees", "sociology", "social", "sciences", "animals", "reproductive", "physiology", "social", "systems", "developmental", "biology", "mutation", "nutrition", "nonsense", "mutation", "sequence", "motif", ...
2019
A genetic switch for worker nutrition-mediated traits in honeybees
Nucleotide-binding oligomerization domain ( NOD ) 2 is a cytosolic protein that plays a defensive role in bacterial infection by sensing peptidoglycans . C5a , which has harmful effects in sepsis , interacts with innate proteins . However , whether NOD2 regulates C5a generation during sepsis remains to be determined . ...
Nucleotide-binding oligomerization domain ( NOD ) 2 is a cytosolic protein that senses peptidoglycans of bacteria and exerts a defensive effect on bacterial infection . Sepsis is a complex dysregulated inflammatory response in bacterial infection , causing multiple organ dysfunction , coagulopathy , and fatal outcome ....
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "bacterial", "diseases", "infectious", "diseases", "bacteremia", "sepsis", "infectious", "disease", "modeling", "critical", "care", "and", "emergency", "medicine" ]
2013
NOD2-mediated Suppression of CD55 on Neutrophils Enhances C5a Generation During Polymicrobial Sepsis
Infectious disease transmission is an inherently spatial process in which a host’s home location and their social mixing patterns are important , with the mixing of infectious individuals often different to that of susceptible individuals . Although incidence data for humans have traditionally been aggregated into low-...
We know that some places have higher rates of infectious disease than others . At the moment , we usually only measure these differences for large towns and cities , though modern data allows us to track movement at much higher resolution . In this paper , we used a computer simulation of an epidemic to propose ways th...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "infectious", "disease", "epidemiology", "influenza", "pathogens", "social", "sciences", "human", "mobility", "population", "biology", "human", "geography", "infectious", "diseases", ...
2019
Differential mobility and local variation in infection attack rate
Visceral leishmaniasis ( VL ) caused by Leishmania donovani remains of public health concern in rural India . Those at risk of VL are also at risk of other neglected tropical diseases ( NTDs ) including soil transmitted helminths . Intestinal helminths are potent regulators of host immune responses sometimes mediated t...
Visceral leishmaniasis ( VL ) , also known as kala-azar , is a potentially fatal disease caused by intracellular parasites of the Leishmania donovani complex . VL is a serious public health problem in rural India , causing high morbidity and mortality , as well as major costs to local and national health budgets . Peop...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "gut", "bacteria", "helminths", "tropical", "diseases", "geographical", "locations", "india", "parasitic", "diseases", "parasitic", "protozoans", "animals", "protozoans", "enterobacteriaceae", "escherichia", "neglect...
2019
Meta-taxonomic analysis of prokaryotic and eukaryotic gut flora in stool samples from visceral leishmaniasis cases and endemic controls in Bihar State India
Surgical technique , including suture placement and tension , is believed to contribute to the outcome of bilamellar tarsal rotation surgery for trachomatous trichiasis . However , the immediate post-operative appearance that minimizes the chance of recurrence and other adverse outcomes has not been investigated . To e...
Trichiasis is a potentially blinding consequence of trachoma . The World Health Organization has promoted the bilamellar tarsal rotation ( BLTR ) procedure as a treatment for trichiasis from trachoma . Even if trachoma were to be eradicated today , a great number of individuals would still develop trichiasis and lose v...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "public", "health", "and", "epidemiology", "epidemiology", "infectious", "disease", "epidemiology", "ophthalmology", "neglected", "tropical", "diseases", "trachoma" ]
2012
Relationship between Immediate Post-Operative Appearance and 6-Week Operative Outcome in Trichiasis Surgery
Enteroviruses ( family of the Picornaviridae ) cover a large group of medically important human pathogens for which no antiviral treatment is approved . Although these viruses have been extensively studied , some aspects of the viral life cycle , in particular morphogenesis , are yet poorly understood . We report the d...
Enteroviruses contain many significant human pathogens , including poliovirus , enterovirus 71 , coxsackieviruses and rhinoviruses . Most enterovirus infections subside mild or asymptomatically , but may also result in severe morbidity and mortality . Here , we report on the mechanism of antiviral action of a small mol...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "antimicrobials", "infectious", "diseases", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "emerging", "infectious", "diseases", "host-pathogen", "interactions", "virology", "emerging", "viral", "diseases", "microbial", "control", "...
2014
Binding of Glutathione to Enterovirus Capsids Is Essential for Virion Morphogenesis
Neutrophils ( PMN ) play a central role in host defense against the neglected fungal infection paracoccidioidomycosis ( PCM ) , which is caused by the dimorphic fungus Paracoccidioides brasiliensis ( Pb ) . PCM is of major importance , especially in Latin America , and its treatment relies on the use of antifungal drug...
PCM triggers a typical granulomatous inflammatory reaction with PMN playing a major role; these inflammatory cells are crucial in the initial stages of PCM , participating in the innate immune reaction and also directing the acquired immune response in the later stages . In some PCM patients , these immune mechanisms a...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Low-level Laser Therapy to the Mouse Femur Enhances the Fungicidal Response of Neutrophils against Paracoccidioides brasiliensis
Fifty years of residual insecticide spraying to control Triatoma infestans in the Gran Chaco region of northern Argentina , Paraguay and Bolivia shows that vertically coordinated interventions aiming at full coverage have limited effects and are unsustainable . We quantified the spatial distribution of T . infestans do...
In the Southern Cone countries of South America , vectorborne transmission of Chagas disease persists in the Gran Chaco region of northern Argentina , Paraguay and Bolivia , where Triatoma infestans is the main vector . More than 50 years of vector control in this region demonstrate that vertically coordinated spraying...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "public", "health", "and", "epidemiology", "geography", "gis", "zoology", "entomology", "epidemiology", "earth", "sciences", "biology", "population", "biology", "spatial", "epidemiology", "cartography" ]
2012
Spatial Heterogeneity and Risk Maps of Community Infestation by Triatoma infestans in Rural Northwestern Argentina
Resistance to macrolide antibiotics is conferred by mutation of A2058 to G or methylation by Erm methyltransferases of the exocyclic N6 of A2058 ( E . coli numbering ) that forms the macrolide binding site in the 50S subunit of the ribosome . Ketolides such as telithromycin mitigate A2058G resistance yet remain suscept...
Bacterial resistance to antibiotics is a serious public health problem that requires the continuous development of new antibiotics . Bacteria acquire resistance to macrolide antibiotics by ( 1 ) effluxing the drug from the cell , ( 2 ) modifying the drug , or ( 3 ) modifying the drug target ( i . e . , the 50S subunit ...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[ "computational", "chemistry", "chemistry", "microbiology", "biology", "biophysics", "computational", "biology" ]
2013
Impact of Ribosomal Modification on the Binding of the Antibiotic Telithromycin Using a Combined Grand Canonical Monte Carlo/Molecular Dynamics Simulation Approach
Infection with dengue virus results in a wide range of clinical manifestations from dengue fever ( DF ) , a self-limited febrile illness , to dengue hemorrhagic fever ( DHF ) which is characterized by plasma leakage and bleeding tendency . Although cardiac involvement has been reported in dengue , the incidence and the...
Dengue is a viral infection with a wide range of symptoms from a self-limiting fever called dengue fever ( DF ) to dengue hemorrhagic fever ( DHF ) which is characterized by leaky blood vessels and bleeding that can lead to shock in severe cases . Abnormal heart function has been reported but the frequencies and the pr...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Evaluation of Cardiac Involvement in Children with Dengue by Serial Echocardiographic Studies
Feature-based attention has a spatially global effect , i . e . , responses to stimuli that share features with an attended stimulus are enhanced not only at the attended location but throughout the visual field . However , how feature-based attention modulates cortical neural responses at unattended locations remains ...
Attentional selection is the mechanism by which relevant sensory information is processed preferentially . Feature-based attention plays a key role in identifying an attentional target in a complex scene , because we often know the features of the target but not its exact location . The ability to quickly select the ta...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "open", "science", "medicine", "and", "health", "sciences", "diagnostic", "radiology", "functional", "magnetic", "resonance", "imaging", "social", "sciences", "light", "electrophysiology", "neuroscience", "electromagnetic", "radiation", "magnetic", "resonance", "imaging", ...
2018
The role of inferior frontal junction in controlling the spatially global effect of feature-based attention in human visual areas
The Wnt receptor Ryk is an evolutionary-conserved protein important during neuronal differentiation through several mechanisms , including γ-secretase cleavage and nuclear translocation of its intracellular domain ( Ryk-ICD ) . Although the Wnt pathway may be neuroprotective , the role of Ryk in neurodegenerative disea...
Neuronal cell decline in neurodegenerative disease can be caused by inherited mutations and involves neuronal dysfunction followed by neuronal death . The ability of neurons to cope with the chronic stress induced by mutant protein expression may determine the course of their decline and eventual demise . Although the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "models", "neurobiology", "of", "disease", "and", "regeneration", "medicine", "and", "health", "sciences", "model", "organisms", "neurology", "biology", "and", "life", "sciences", "neuroscience", "research", "and", "analysis", "methods" ]
2014
The Wnt Receptor Ryk Reduces Neuronal and Cell Survival Capacity by Repressing FOXO Activity During the Early Phases of Mutant Huntingtin Pathogenicity
Chagas disease , considered a neglected disease by the World Health Organization , is caused by the protozoan parasite Trypanosoma cruzi , and transmitted by >140 triatomine species across the Americas . In Central America , the main vector is Triatoma dimidiata , an opportunistic blood meal feeder inhabiting both dome...
Chagas disease is caused by the parasite Trypanosoma cruzi , which is spread by triatomine kissing bugs . There are many biotic factors that influence the risk of disease transmission , including the strain of the parasite , the vector movement patterns , the community of microbes interacting with the parasite inside t...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Conclusions" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "abdomen", "geographical", "locations", "vector-borne", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "invertebrate", "genomics", "north", "america", "protozoans", "central", "america", "guatemal...
2018
Uncovering vector, parasite, blood meal and microbiome patterns from mixed-DNA specimens of the Chagas disease vector Triatoma dimidiata
Fungal pathogens exploit diverse mechanisms to survive exposure to antifungal drugs . This poses concern given the limited number of clinically useful antifungals and the growing population of immunocompromised individuals vulnerable to life-threatening fungal infection . To identify molecules that abrogate resistance ...
Treating fungal infections is challenging due to the emergence of drug resistance and the limited number of clinically useful antifungal drugs . We screened a library of 1 , 280 pharmacologically active compounds to identify those that reverse resistance of the leading human fungal pathogen , Candida albicans , to the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/fungal", "infections", "infectious", "diseases/antimicrobials", "and", "drug", "resistance" ]
2010
PKC Signaling Regulates Drug Resistance of the Fungal Pathogen Candida albicans via Circuitry Comprised of Mkc1, Calcineurin, and Hsp90
Although leptospirosis is a zoonosis of major concern on tropical islands , the molecular epidemiology of the disease aiming at linking human cases to specific animal reservoirs has been rarely explored within these peculiar ecosystems . Five species of wild small mammals ( n = 995 ) as well as domestic animals ( n = 1...
Leptospirosis is a zoonosis caused by infection with pathogenic Leptospira species . A broad range of animals , including rodents , pets and livestock , are maintenance hosts for leptospires . However , assessing the relative importance of each host in the contamination of the environment and , in fine , in the infecti...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "leptospira", "pathology", "and", "laboratory", "medicine", "pathogens", "tropical", "diseases", "microbiology", "vertebrates", "animals", "mammals", "dogs", "bacterial", "diseases", "neglected", "tropical", "diseases", "molecular"...
2016
Human Leptospirosis on Reunion Island, Indian Ocean: Are Rodents the (Only) Ones to Blame?
It is currently believed that type I and III interferons ( IFNs ) have redundant functions . However , the preferential distribution of type III IFN receptor on epithelial cells suggests functional differences at epithelial surfaces . Here , using human intestinal epithelial cells we could show that although both type ...
The human intestinal tract plays two important roles in the body: first it is responsible for nutrient absorption and second it is the primary barrier which protects the human body from the outside environment . This complex tissue is constantly exposed to commensal bacteria and is often exposed to both bacterial and v...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "organoids", "medicine", "and", "health", "sciences", "luciferase", "vesicular", "stomatitis", "virus", "pathology", "and", "laboratory", "medicine", "enzymes", "pathogens", "biological", "cultures", "enzymology", "microbiology", "viruses", "rna", "viruses", "organ", "c...
2018
Differential induction of interferon stimulated genes between type I and type III interferons is independent of interferon receptor abundance
Dengue hemorrhagic fever and dengue shock syndrome ( DHF/DSS ) are life-threatening complications following infection with one of the four serotypes of dengue virus ( DENV ) . At present , no vaccine or antiviral therapies are available against dengue . Here , we characterized a panel of eight human or mouse-human chim...
The four dengue virus serotypes ( DENV1-4 ) cause the most prevalent mosquito-transmitted viral disease globally , infecting 50–100 million people annually in tropical and sub-tropical regions worldwide , yet no vaccine or therapy has been licensed to prevent or treat dengue . The greatest risk factor for severe dengue...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "humoral", "immunity", "medicine", "infectious", "diseases", "immunity", "dengue", "fever", "neglected", "tropical", "diseases", "immunology", "biology", "viral", "diseases", "dengue", "immune", "response" ]
2013
Therapeutic Efficacy of Antibodies Lacking FcγR against Lethal Dengue Virus Infection Is Due to Neutralizing Potency and Blocking of Enhancing Antibodies
Yersinia pseudotuberculosis forms biofilms on Caenorhabditis elegans which block nematode feeding . This genetically amenable host-pathogen model has important implications for biofilm development on living , motile surfaces . Here we show that Y . pseudotuberculosis biofilm development on C . elegans is governed by N-...
Many Gram-negative bacteria communicate by producing and sensing the presence of chemical signal molecules such as the N-acylhomoserine lactones ( AHLs ) . Bacterial cells use AHLs to convey information about their environment , metabolism and population size . This type of chemical signalling is called ‘quorum sensing...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology", "microbiology/cellular", "microbiology", "and", "pathogenesis", "microbiology" ]
2011
Biofilm Development on Caenorhabditis elegans by Yersinia Is Facilitated by Quorum Sensing-Dependent Repression of Type III Secretion
The prediction of phenotypic traits using high-density genomic data has many applications such as the selection of plants and animals of commercial interest; and it is expected to play an increasing role in medical diagnostics . Statistical models used for this task are usually tested using cross-validation , which imp...
The availability of increasing amounts of genomic data is making the use of statistical models to predict traits of interest a mainstay of many applications in life sciences . Applications range from medical diagnostics for common and rare diseases to breeding characteristics such as disease resistance in plants and an...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biotechnology", "population", "genetics", "cereal", "crops", "plant", "science", "mathematics", "forecasting", "statistics", "(mathematics)", "crops", "plant", "genomics", "mammalian", "genomics", "population", "biology", "plants", "research", "and", "analysis", "methods...
2016
Using Genetic Distance to Infer the Accuracy of Genomic Prediction
Onchocerciasis or "river blindness" is a chronic parasitic neglected tropical disease which is endemic both in mainland and insular Equatorial Guinea . We aim to estimate the current epidemiological situation of onchocerciasis in Bioko Island after vector elimination in 2005 and more than sixteen years of Community Dir...
Onchocerciasis or “river blindness” is a chronic parasitic disease which is mainly found in Sub-Saharan Africa . Onchocerciasis is endemic in both mainland and insular Equatorial Guinea . Huge achievements have been made on onchocerciasis control in Bioko Island in the last years , and the country is moving fast toward...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "onchocerca", "volvulus", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "geographical", "locations", "parasitic", "diseases", "animals", "onchocerca", "parasit...
2016
Evidence for Suppression of Onchocerciasis Transmission in Bioko Island, Equatorial Guinea
Unlike mammals and birds , teleost fish undergo external embryogenesis , and therefore their embryos are constantly challenged by stresses from their living environment . These stresses , when becoming too harsh , will cause arrest of cell proliferation , abnormal cell death or senescence . Such organisms have to evolv...
Although being challenged by stresses from their living environment during embryogenesis , teleost fish harbor a robust genetic program dictating liver development as long as any environmental change , including temperature or natural UV irradiation , is not detrimental . It is therefore of interest to explore the mech...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "protein", "transport", "cell", "processes", "vertebrates", "animals", "animal", "models", "osteichthyes", "developmental", "biology", "model", "organisms", "organism", "development", "glycosylation", "embryos", "extraction", "techniques", "research", "and", "analysis", "...
2016
Liver-Enriched Gene 1, a Glycosylated Secretory Protein, Binds to FGFR and Mediates an Anti-stress Pathway to Protect Liver Development in Zebrafish
Analyses investigating low frequency variants have the potential for explaining additional genetic heritability of many complex human traits . However , the natural frequencies of rare variation between human populations strongly confound genetic analyses . We have applied a novel collapsing method to identify biologic...
Low frequency variants are likely to play an important role in uncovering complex trait heritability; however , they are often continent or population specific . This specificity complicates genetic analyses investigating low frequency variants for two reasons: low frequency variant signals in an association test are o...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genome-wide", "association", "studies", "genome", "sequencing", "genome", "analysis", "tools", "biology", "genomics", "computational", "biology" ]
2013
Low Frequency Variants, Collapsed Based on Biological Knowledge, Uncover Complexity of Population Stratification in 1000 Genomes Project Data
In the glucose-free environment that is the midgut of the tsetse fly vector , the procyclic form of Trypanosoma brucei primarily uses proline to feed its central carbon and energy metabolism . In these conditions , the parasite needs to produce glucose 6-phosphate ( G6P ) through gluconeogenesis from metabolism of non-...
Trypanosoma brucei , the parasite responsible for sleeping sickness in humans , is transmitted by the tsetse fly that primarily uses amino acids for its energy production . In the glucose-free environment encountered between the insect blood meals , T . brucei needs to produce through gluconeogenesis glucose 6-phosphat...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "carbohydrate", "metabolism", "medicine", "and", "health", "sciences", "chemical", "compounds", "monomers", "phosphates", "carbohydrates", "glucose", "metabolism", "organic", "compounds", "parasitic", "protozoans", "glucose", "parasitic", "diseases", "protozoans", "amino", ...
2018
Gluconeogenesis is essential for trypanosome development in the tsetse fly vector
The nonsense-mediated decay ( NMD ) pathway subjects mRNAs with premature termination codons ( PTCs ) to rapid decay . The conserved Upf1–3 complex interacts with the eukaryotic translation release factors , eRF3 and eRF1 , and triggers NMD when translation termination takes place at a PTC . Contrasting models postulat...
The nonsense-mediated mRNA decay pathway is responsible for rapidly degrading mRNAs with premature termination codons . This is important because it prevents the production of potentially deleterious truncated proteins from aberrant mRNAs , such as those that have undergone erroneous processing . How does the cell disc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology" ]
2008
A Competition between Stimulators and Antagonists of Upf Complex Recruitment Governs Human Nonsense-Mediated mRNA Decay
Glycosylation of viral envelope proteins is important for infectivity and interaction with host immunity , however , our current knowledge of the functions of glycosylation is largely limited to N-glycosylation because it is difficult to predict and identify site-specific O-glycosylation . Here , we present a novel pro...
Information on site-specific O-glycosylation of viral envelope glycoproteins is generally very limited despite important functions . We present a powerful mass-spectrometry based strategy to globally identify O-glycosylation sites on viral envelope proteins of a given virus in the context of a productive infection . We...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
A Strategy for O-Glycoproteomics of Enveloped Viruses—the O-Glycoproteome of Herpes Simplex Virus Type 1
Cerebral malaria ( CM ) is a severe complication of Plasmodium falciparum infection that results in thousands of deaths each year , mostly in African children . The in vivo mechanisms underlying this fatal condition are not entirely understood . Using the animal model of experimental cerebral malaria ( ECM ) , we sough...
Cerebral malaria ( CM ) is a severe and potentially fatal complication of malaria in humans that results in swelling and bleeding within the brain . The mechanisms that cause this fatal disease in humans are not completely understood . We studied an animal model known as experimental cerebral malaria to learn more abou...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "cell", "death", "medicine", "and", "health", "sciences", "immune", "cells", "cardiovascular", "anatomy", "nervous", "system", "immunology", "tropical", "diseases", "brain", "cell", "processes", "parasitic", "diseases", "neuronal", "death", "cytotoxi...
2016
CD8+ T Cells Induce Fatal Brainstem Pathology during Cerebral Malaria via Luminal Antigen-Specific Engagement of Brain Vasculature
Chronic kidney disease ( CKD ) is a worldwide public health problem that is associated with substantial morbidity and mortality . To search for sequence variants that associate with CKD , we conducted a genome-wide association study ( GWAS ) that included a total of 3 , 203 Icelandic cases and 38 , 782 controls . We ob...
Chronic kidney disease ( CKD ) is a common condition that is associated with substantial morbidity and mortality and has been recognized as a major public health problem worldwide . Common causes of CKD include hypertension , diabetes , and inflammatory disorders . Previous studies have shown a significant genetic cont...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genetics", "of", "disease", "genetics", "and", "genomics/gene", "discovery", "genetics", "and", "genomics/population", "genetics" ]
2010
Association of Variants at UMOD with Chronic Kidney Disease and Kidney Stones—Role of Age and Comorbid Diseases
Host protection from fungal infection is thought to ensue in part from the activity of Syk-coupled C-type lectin receptors and MyD88-coupled toll-like receptors in myeloid cells , including neutrophils , macrophages and dendritic cells ( DCs ) . Given the multitude of cell types and receptors involved , elimination of ...
Multiple cell types bearing a vast array of immune receptors with different modes of signaling ensure that the host response to infection is both robust and reliable . For this reason , loss of a single signaling pathway in a given cell type is often not enough to impact host resistance . Here , we find , surprisingly ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "developmental", "biology", "innate", "immune", "system", "white", "blood", "cells", "immune", "cells", "cytokines", "cell", "biology", "animal", "cells", "nk", "cells", "immunity", "molecular", "development", "antigen-presenting", "cells", "immunity...
2014
Syk Signaling in Dendritic Cells Orchestrates Innate Resistance to Systemic Fungal Infection
Apolipoprotein E ( apoE ) is a forefront actor in the transport of lipids and the maintenance of cholesterol homeostasis , and is also strongly implicated in Alzheimer’s disease . Upon lipid-binding apoE adopts a conformational state that mediates the receptor-induced internalization of lipoproteins . Due to its inhere...
Among the proteins involved in the transport of lipids and their distribution to the cells , apolipoprotein E ( apoE ) mediates the internalization of cholesterol rich lipoproteins by acting as a ligand for cell-surface receptors . In the central nervous system , while apoE is the major cholesterol transport protein , ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "pattern", "recognition", "receptors", "medicine", "and", "health", "sciences", "molecular", "dynamics", "immunology", "lipoprotein", "receptors", "lipid", "structure", "immune", "system", "proteins", "lipids", "proteins", "lipoproteins", "chemistry", "physics", "biochemi...
2018
Lipidated apolipoprotein E4 structure and its receptor binding mechanism determined by a combined cross-linking coupled to mass spectrometry and molecular dynamics approach
Bacterial whole genome sequencing holds promise as a disruptive technology in clinical microbiology , but it has not yet been applied systematically or comprehensively within a clinical context . Here , over the course of one year , we performed prospective collection and whole genome sequencing of nearly all bacterial...
Bacterial whole genome sequencing is becoming increasingly common to microbiological research , but despite its great potential , has not yet been meaningfully integrated into clinical care . Here , we generated whole genome sequencing data from nearly all of the bacterial isolates prospectively collected from a hospit...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
A Year of Infection in the Intensive Care Unit: Prospective Whole Genome Sequencing of Bacterial Clinical Isolates Reveals Cryptic Transmissions and Novel Microbiota
Lymphoid oncogenesis is a life threatening complication associated with a number of persistent viral infections ( e . g . EBV and HTLV-1 in humans ) . With many of these infections it is difficult to study their natural history and the dynamics of tumor formation . Marek's Disease Virus ( MDV ) is a prevalent α-herpesv...
Many viral infections target the immune system , making use of the long lived , highly proliferative lymphocytes to propagate and survive within the host . This characteristic has led to an association between some viruses such as Epstein Barr Virus ( EBV ) , Human T cell Lymphotrophic Virus-1 ( HTLV-1 ) and Mareks Dis...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "immunology/cellular", "microbiology", "and", "pathogenesis", "physiology/immunity", "to", "infections", "virology/animal", "models", "of", "infection", "infectious", "diseases/viral", "infections", "virology/viruses", "and", "cancer", "pathology/immunology", "oncology/myelomas",...
2011
Clonal Structure of Rapid-Onset MDV-Driven CD4+ Lymphomas and Responding CD8+ T Cells
A complete understanding of the mechanisms underlying the acquisition of protective immunity is crucial to improve vaccine strategies to eradicate malaria . However , it is still unclear whether recognition of damage signals influences the immune response to Plasmodium infection . Adenosine triphosphate ( ATP ) accumul...
Malaria still causes the death of approximately half a million people yearly despite efforts to develop vaccines . The ability of Plasmodium parasites to survive the immune effector mechanisms indicates how suitable the immune response must be to eliminate the infection . CD4 T cells have a dual role in protection agai...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "t", "helper", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "spleen", "immunology", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "cell", "differentiation", "parasitology", ...
2017
P2X7 receptor drives Th1 cell differentiation and controls the follicular helper T cell population to protect against Plasmodium chabaudi malaria
The human pathogen Vibrio cholerae is an aquatic bacterium frequently encountered in rivers , lakes , estuaries , and coastal regions . Within these environmental reservoirs , the bacterium is often found associated with zooplankton and more specifically with their chitinous exoskeleton . Upon growth on such chitinous ...
The human pathogen Vibrio cholerae is an aquatic bacterium often encountered in rivers , estuaries , and coastal regions . Within this environmental niche , the bacterium often associates with the chitinous exoskeleton of zooplankton . Upon colonization of these chitinous surfaces , V . cholerae switches on a developme...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "bacteriology", "gene", "networks", "gene", "regulation", "microbiology", "microbial", "evolution", "molecular", "genetics", "microbial", "growth", "and", "development", "bacterial", "pathogens", "biology", "microbial", "ecology", "bacterial", "physiology", "gram", "negat...
2012
The Regulatory Network of Natural Competence and Transformation of Vibrio cholerae
Epithelial homeostasis in the posterior midgut of Drosophila is maintained by multipotent intestinal stem cells ( ISCs ) . ISCs self-renew and produce enteroblasts ( EBs ) that differentiate into either enterocytes ( ECs ) or enteroendocrine cells ( EEs ) in response to differential Notch ( N ) activation . Various env...
Stem cells maintain tissue homeostasis in metazoans . A productive model to study the regulation of stem cell function is the Drosophila posterior midgut . Notch ( N ) signaling controls intestinal stem cell ( ISC ) differentiation in this tissue , while ISC proliferation is regulated by growth factor signaling pathway...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "insulin-dependent", "signal", "transduction", "mitogenic", "signaling", "tor", "signaling", "cell", "differentiation", "animal", "models", "developmental", "biology", "drosophila", "melanogaster", "model", "organisms", "stem", "cells", "cell", "growth", "signaling", "pat...
2012
Notch-Mediated Suppression of TSC2 Expression Regulates Cell Differentiation in the Drosophila Intestinal Stem Cell Lineage
Synthetic lethality is a genetic interaction wherein two otherwise nonessential genes cause cellular inviability when knocked out simultaneously . Drugs can mimic genetic knock-out effects; therefore , our understanding of promiscuous drugs , polypharmacology-related adverse drug reactions , and multi-drug therapies , ...
Synthetic lethality is a genetic interaction that has promising implications for informing novel cancer therapies . Over 200 million pairwise tests would be required to identify all pairwise synthetic lethal interactions in humans–currently , an impossibly large experimental burden . To simplify the process , we have d...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Connectivity Homology Enables Inter-Species Network Models of Synthetic Lethality
Yeast WHI2 was originally identified in a genetic screen for regulators of cell cycle arrest and later suggested to function in general stress responses . However , the function of Whi2 is unknown . Whi2 has predicted structure and sequence similarity to human KCTD family proteins , which have been implicated in severa...
Yeast and human cells respond to declining levels of available nutrients to prepare ahead for leaner times . The detailed mechanisms of nutrient sensing are not well understood , but defects in these processes have key roles in diseases such as cancer . The evolutionarily conserved protein complex TORC1 is the control ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "chemical", "compounds", "molecular", "probe", "techniques", "enzymes", "enzymology", "immunoblotting", "carbohydrates", "organic", "compounds", "glucose", "fungi", "model", "organisms", "experimental", "organism", "systems", "molecular", "biology", "tech...
2018
Whi2 is a conserved negative regulator of TORC1 in response to low amino acids
China has the highest incidence of hemorrhagic fever with renal syndrome ( HFRS ) worldwide . Reported cases account for 90% of the total number of global cases . By 2010 , approximately 1 . 4 million HFRS cases had been reported in China . This study aimed to explore the effect of the rodent reservoir , and natural an...
Hemorrhagic fever with renal syndrome ( HFRS ) , a rodent-borne disease caused by hantaviruses , is characterized by fever , haemorrhage , headache , back pain , abdominal pain , and acute kidney injury . China has the highest incidence of HFRS worldwide . Reported cases account for 90% of the total global cases . Appr...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "environmental", "epidemiology", "epidemiology", "neglected", "tropical", "diseases" ]
2014
Animal Reservoir, Natural and Socioeconomic Variations and the Transmission of Hemorrhagic Fever with Renal Syndrome in Chenzhou, China, 2006–2010
The Escherichia coli mazEF module is one of the most thoroughly studied toxin–antitoxin systems . mazF encodes a stable toxin , MazF , and mazE encodes a labile antitoxin , MazE , which prevents the lethal effect of MazF . MazF is an endoribonuclease that leads to the inhibition of protein synthesis by cleaving mRNAs a...
The enteric bacterium E . coli , as most other bacteria , carries a pair of genes on its chromosome; one of them specifies a toxin and the other one an antitoxin . Previously , we have shown that that the mazEF toxin–antitoxin system in E . coli is responsible for bacterial cell death under stressful conditions . Clear...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "discovery", "genetics", "and", "genomics/gene", "function", "molecular", "biology/translational", "regulation", "microbiology", "microbiology/microbial", "physiology", "and", "metabolism", "molecular", "biology", "genetics", "and", "genomi...
2009
Escherichia coli MazF Leads to the Simultaneous Selective Synthesis of Both “Death Proteins” and “Survival Proteins”
It is well documented that the density of Plasmodium in its vertebrate host modulates the physiological response induced; this in turn regulates parasite survival and transmission . It is less clear that parasite density in the mosquito regulates survival and transmission of this important pathogen . Numerous studies h...
Malaria , one of the world's most devastating parasitic diseases , is caused by protozoan parasites of the genus Plasmodium and is transmitted between mammalian hosts by Anopheles mosquitoes . Within the mosquito , the parasite undergoes four sequential developmental transformations as it passes from the bloodmeal thro...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "mosquito", "infectious", "diseases", "malaria", "dynamics" ]
2007
Progression of Plasmodium berghei through Anopheles stephensi Is Density-Dependent
Pulmonary cavities , the hallmark of tuberculosis ( TB ) , are characterized by high mycobacterial load and perpetuate the spread of M . tuberculosis . The mechanism of matrix destruction resulting in cavitation is not well defined . Neutrophils are emerging as key mediators of TB immunopathology and their influx are a...
Neutrophil infiltration is characteristic of immune-induced pathology in tuberculosis but mechanisms whereby neutrophils cause tissue destruction are not fully understood . In this study , we show that neutrophils secrete the collagenase MMP-8 in response to direct infection with Mycobacterium tuberculosis and via cell...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Neutrophil-Derived MMP-8 Drives AMPK-Dependent Matrix Destruction in Human Pulmonary Tuberculosis
The TEAD family of transcription factors ( TEAD1-4 ) bind the MCAT element in the regulatory elements of both growth promoting and myogenic differentiation genes . Defining TEAD transcription factor function in myogenesis has proved elusive due to overlapping expression of family members and their functional redundancy...
Aspects of muscle differentiation can be reproduced using the C2C12 cell line or primary myoblasts both of which can be differentiated to form myotubes in vitro . While the functions of recognised myogenic proteins such as Myogenin , Myod1 and MEF-family transcription factors in this process have been extensively studi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "gene", "regulation", "cell", "differentiation", "muscle", "regeneration", "developmental", "biology", "organism", "development", "stem", "cells", "morphogenesis", "small", "interfering", "rnas", "muscle", "differentiation", "animal", "cells", "gene", "expression", "myobl...
2017
TEAD transcription factors are required for normal primary myoblast differentiation in vitro and muscle regeneration in vivo
In Drosophila , multiple lines of evidence converge in suggesting that beneficial substitutions to the genome may be common . All suffer from confounding factors , however , such that the interpretation of the evidence—in particular , conclusions about the rate and strength of beneficial substitutions—remains tentative...
Characterizing the nature of beneficial changes to the genome is essential to our understanding of adaptation . To do so , researchers identify and analyze footprints that beneficial changes leave in patterns of genetic variation within and between species . In order to teach us about adaptive evolution , these footpri...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology", "genetics", "and", "genomics" ]
2011
Pervasive Adaptive Protein Evolution Apparent in Diversity Patterns around Amino Acid Substitutions in Drosophila simulans
There is a popular belief in neuroscience that we are primarily data limited , and that producing large , multimodal , and complex datasets will , with the help of advanced data analysis algorithms , lead to fundamental insights into the way the brain processes information . These datasets do not yet exist , and if the...
Neuroscience is held back by the fact that it is hard to evaluate if a conclusion is correct; the complexity of the systems under study and their experimental inaccessability make the assessment of algorithmic and data analytic technqiues challenging at best . We thus argue for testing approaches using known artifacts ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "nervous", "system", "computer", "hardware", "light", "neuroscience", "electromagnetic", "radiation", "luminance", "brain", "mapping", "computational", "neuroscience", "visible", "light", "neuronal", "tuning", "computer", "and", ...
2017
Could a Neuroscientist Understand a Microprocessor?
The Pantanal is a hotspot for arbovirus studies in South America . Various medically important flaviviruses and alphaviruses have been reported in domestic and wild animals in the region . To expand the knowledge of local arbovirus circulation , a serosurvey for 14 Brazilian orthobunyaviruses was conducted with equines...
In the present study , we report the evidence of various orthobunyaviruses of medical importance in domestic and wild animals of the Pantanal , a large floodplain located in West-Central Brazil . Although various other arboviruses as flaviviruses and alphaviruses have been reported in the region , orthobunyaviruses are...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "ruminants", "pathogens", "immunology", "geographical", "locations", "microbiology", "vertebrates", "animals", "mammals", "viruses", "rna", "vir...
2017
Neutralizing antibodies for orthobunyaviruses in Pantanal, Brazil
The success of combination antiretroviral therapy is limited by the evolutionary escape dynamics of HIV-1 . We used Isotonic Conjunctive Bayesian Networks ( I-CBNs ) , a class of probabilistic graphical models , to describe this process . We employed partial order constraints among viral resistance mutations , which gi...
Drug resistance remains a challenge in the management of HIV-infected patients . The accumulation of mutations during ongoing viral replication is the origin of drug resistance development . Understanding this evolutionary process in a quantitative manner is an important prerequisite for minimizing the risk of resistan...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods", "Acknowledgments" ]
[]
2013
The Individualized Genetic Barrier Predicts Treatment Response in a Large Cohort of HIV-1 Infected Patients
All eukaryotic genomes are packaged as chromatin , with DNA interlaced with both regularly patterned nucleosomes and sub-nucleosomal-sized protein structures such as mobile and labile transcription factors ( TF ) and initiation complexes , together forming a dynamic chromatin landscape . Whilst details of nucleosome po...
DNA is packaged by proteins into chromatin , of which the fundamental unit is a complex of histone proteins that wraps ~150bp of DNA into a nucleosome . Digestion of chromatin with enzymes such as micrococcal nuclease cuts the DNA between the protein particles , and by sequencing the cut sites , they can be mapped acro...
[ "Abstract", "Introduction", "Results", "&", "discussion", "Materials", "&", "methods" ]
[ "biotechnology", "brassica", "nucleosome", "mapping", "plant", "science", "model", "organisms", "experimental", "organism", "systems", "plant", "genomics", "epigenetics", "molecular", "biology", "techniques", "plants", "chromatin", "structural", "genomics", "research", "a...
2017
Genome-wide chromatin mapping with size resolution reveals a dynamic sub-nucleosomal landscape in Arabidopsis
During neural circuit formation , most axons are guided to complex environments , coming into contact with multiple potential synaptic partners . However , it is critical that they recognize specific neurons with which to form synapses . Here , we utilize the split GFP-based marker Neuroligin-1 GFP Reconstitution Acros...
The nervous system is required for many body functions including perception , behavior and thought . Cells in the nervous system called neurons function in interconnected groups called circuits to carry out these basic functions . While we have learned a great deal about how circuits function , we know much less about ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "nervous", "system", "enzymes", "caenorhabditis", "enzymology", "electrophysiology", "neurites", "neuroscience", "animals", "phosphatases", "animal", "models", "caenorhabditis", "elegans", "model", "organisms", "anim...
2018
The receptor protein tyrosine phosphatase CLR-1 is required for synaptic partner recognition
Hedgehog signaling plays conserved roles in controlling embryonic development; its dysregulation has been implicated in many human diseases including cancers . Hedgehog signaling has an unusual reception system consisting of two transmembrane proteins , Patched receptor and Smoothened signal transducer . Although activ...
Hedgehog ( Hh ) signaling is a pathway renowned for its roles in controlling embryonic development and tumorigenesis . Signaling via this pathway proceeds when Hh ligands bind to the receptor Patched ( Ptc ) , thereby preventing Ptc from inhibiting the signal transducer , Smoothened ( Smo ) , and thus allowing Smo to a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Activation of Smurf E3 Ligase Promoted by Smoothened Regulates Hedgehog Signaling through Targeting Patched Turnover
A comprehensive analysis was done to evaluate the potential use of anti-parasitic macrocyclic lactones ( including avermectins and milbemycins ) for Buruli ulcer ( BU ) therapy . A panel containing nearly all macrocyclic lactones used in human or in veterinary medicine was analyzed for activity in vitro against clinica...
Buruli ulcer ( BU ) is a chronic debilitating mycobacterial disease of the skin and soft tissue caused by Mycobacterium ulcerans . It is mainly found in tropical regions and often linked to poverty . BU can be cured in most cases with the standard treatment , a combination of rifampicin and the injectable antibiotic st...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Selamectin Is the Avermectin with the Best Potential for Buruli Ulcer Treatment
The non-specific symptoms of Ebola Virus Disease ( EVD ) pose a major problem to triage and isolation efforts at Ebola Treatment Centres ( ETCs ) . Under the current triage protocol , half the patients allocated to high-risk “probable” wards were EVD ( - ) : a misclassification speculated to predispose nosocomial EVD i...
Four decades after the discovery of Ebola virus disease ( EVD ) , the sources , reservoirs and dynamics of infection are still largely unknown and thus the threat of re-emergence remains ever present . As EVD thrives on fragile healthcare systems in the developing world , it is essential that triage tools are low-cost ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Conclusion" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "geographical", "locations", "ebola", "hemorrhagic", "fever", "parasitic", "diseases", "diarrhea", "signs", "and", "symptoms", "gastroenterology", "and", "hepa...
2017
Predicting Ebola infection: A malaria-sensitive triage score for Ebola virus disease
Stability in a metabolic system may not be obtained if incorrect amounts of enzymes are used . Without stability , some metabolites may accumulate or deplete leading to the irreversible loss of the desired operating point . Even if initial enzyme amounts achieve a stable steady state , changes in enzyme amount due to s...
A method of metabolic simulation called ensemble modelling for robustness analysis is used to predict the behavior intrinsic to the network structure ( stoichiometry and kinetic form ) of four enzymatic systems . Some network structures are shown to be prone to instability . Starting from a stable system , instability ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "chemical", "compounds", "enzymes", "metabolic", "processes", "ketones", "enzymology", "carbohydrates", "organic", "compounds", "glucose", "glycolysis", "pyruvate", "systems", "science", "mathematics", "metabolites", "enzyme", "metabolism", "enzyme", "chemistry", "computer"...
2016
Stability of Ensemble Models Predicts Productivity of Enzymatic Systems
The female gametophyte of flowering plants , the embryo sac , develops within the diploid ( sporophytic ) tissue of the ovule . While embryo sac–expressed genes are known to be required at multiple stages of the fertilization process , the set of embryo sac–expressed genes has remained poorly defined . In particular , ...
During the sexual reproduction of flowering plants , a pollen tube delivers sperm cells to a specialized group of cells known as the embryo sac , which contains the egg cell . It is known that embryo sacs are active participants in guiding the growth of pollen tubes , in facilitating fertilization , and in initiating s...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "plants", "genetics", "and", "genomics", "arabidopsis", "(thale", "cress)" ]
2007
Genome-Wide Expression Profiling of the Arabidopsis Female Gametophyte Identifies Families of Small, Secreted Proteins
RNA polymerase II synthesizes a diverse set of transcripts including both protein-coding and non-coding RNAs . One major difference between these two classes of transcripts is the mechanism of termination . Messenger RNA transcripts terminate downstream of the coding region in a process that is coupled to cleavage and ...
Transcription in eukaryotes is widespread including both protein-coding transcripts and an increasing number of non-coding RNAs . Here we present the results of transcriptome-wide mapping of a set of yeast RNA–binding proteins that control expression of some protein-coding genes and a number of novel non-coding RNAs . ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome", "expression", "analysis", "molecular", "cell", "biology", "rna", "processing", "gene", "regulation", "genetics", "gene", "expression", "molecular", "genetics", "biology", "genomics", "molecular", "biology", "genetics", "and", "genomics", "dna", "transcription"...
2011
Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-Poly(A) Termination Pathway: Nrd1, Nab3, and Sen1
Expansion of DNA trinucleotide repeats causes at least 15 hereditary neurological diseases , and these repeats also undergo contraction and fragility . Current models to explain this genetic instability invoke erroneous DNA repair or aberrant replication . Here we show that CAG/CTG tracts are stabilized in Saccharomyce...
DNA trinucleotide repeats are naturally occurring runs of three base-pairs . Genetic mutations that expand ( lengthen ) triplet repeats cause multiple neurological diseases , including Huntington's disease . Triplet repeats also contract ( shorten ) and break . This complex behavior suggests triplet repeats are problem...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "molecular", "biology/dna", "replication", "molecular", "biology/recombination", "genetics", "and", "genomics/chromosome", "biology", "genetics", "and", "genomics/disease", "models", "genetics", "and", "genomics/gene", "function", "molecular", "biology/dna", "repair" ]
2011
New Functions of Ctf18-RFC in Preserving Genome Stability outside Its Role in Sister Chromatid Cohesion
Metabolic network reconstructions represent valuable scaffolds for ‘-omics’ data integration and are used to computationally interrogate network properties . However , they do not explicitly account for the synthesis of macromolecules ( i . e . , proteins and RNA ) . Here , we present the first genome-scale , fine-grai...
Systems biology aims to understand the interactions of cellular components in a systemic manner . Mathematical modeling is critical to the integration and analysis of these components on a conceptual as well as mechanistic level . To date , detailed genome-scale reconstructions of metabolism have become available for a...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/microbial", "physiology", "and", "metabolism", "computational", "biology/literature", "analysis", "cell", "biology/microbial", "growth", "and", "development", "molecular", "biology/bioinformatics", "computational", "biology/metabolic", "networks", "computational"...
2009
Genome-Scale Reconstruction of Escherichia coli's Transcriptional and Translational Machinery: A Knowledge Base, Its Mathematical Formulation, and Its Functional Characterization
Dengue is one of the most common infectious diseases . The aim of this study was to systematically review acute disseminated encephalomyelitis ( ADEM ) and to represent a new case . We searched for articles in nine databases for case reports , series or previous reviews reporting ADEM cases in human . We used Fisher’s ...
We presented a 13-year-old girl of ADEM following dengue infection . She was totally alert and had a low grade of fever with no focal neurologic deficits , on admission . We revealed that the prevalence of either ADEM or all neurological disorders among dengue patients was not too rare . Moreover , we found that the mo...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "diagnostic", "radiology", "pathology", "and", "laboratory", "medicine", "vomiting", "tropical", "diseases", "headaches", "magnetic", "resonance", "imaging", "urine", "physiological", "processes", "signs", "and"...
2017
Post-dengue acute disseminated encephalomyelitis: A case report and meta-analysis
The characterization of functional elements in genomes relies on the identification of the footprints of natural selection . In this quest , taking into account neutral evolutionary processes such as mutation and genetic drift is crucial because these forces can generate patterns that may obscure or mimic signatures of...
Classical population genetics models indicate that the efficiency of selection , and hence adaptation , depends on a number of non-selective factors , such as the size of a population or the intensity of recombination . In the last 10 years , evidence has accumulated that another mechanism called GC-Biased Gene Convers...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
GC-Content Evolution in Bacterial Genomes: The Biased Gene Conversion Hypothesis Expands
We evaluated the profile of patients referred to the Fiocruz Outpatient Clinic , a reference center for the diagnosis and treatment of leprosy in Rio de Janeiro , RJ , and analyzed the origins and outcomes of these referrals . This is an observational retrospective study based on information collected from the Leprosy ...
Leprosy , a neglected disease , remains endemic in some developing countries despite the existence of a successful program to treat and cure patients . While has been a drastic decrease in the number of patients , but we still have a stable number of new cases that is still very high in countries like India and Brazil ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "dermatology", "medicine", "and", "health", "sciences", "outpatient", "clinics", "disabilities", "biopsy", "tropical", "diseases", "geographical", "locations", "surgical", "and", "invasive", "medical", "procedures", "health", "care", "bacterial", "diseases", "neglected", ...
2016
Impact of a Reference Center on Leprosy Control under a Decentralized Public Health Care Policy in Brazil
Many interspecies hybrids have been discovered in yeasts , but most of these hybrids are asexual and can replicate only mitotically . Whole-genome duplication has been proposed as a mechanism by which interspecies hybrids can regain fertility , restoring their ability to perform meiosis and sporulate . Here , we show t...
It has recently been proposed that the whole-genome duplication ( WGD ) event that occurred during evolution of an ancestor of the yeast S . cerevisiae was the result of a hybridization between 2 parental yeast species that were significantly divergent in DNA sequence , followed by a doubling of the genome content to r...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "fungal", "spores", "chromosome", "structure", "and", "function", "centromeres", "sequence", "assembly", "tools", "fungi", "model", "organisms", "experimental", "organism", "systems", "genome", "analysis", "fungal", "reproduction", "saccharomyces", "research", "and", "a...
2017
Evolutionary restoration of fertility in an interspecies hybrid yeast, by whole-genome duplication after a failed mating-type switch
Asymmetric division of zygote is critical for pattern formation during early embryogenesis in plants and animals . It requires integration of the intrinsic and extrinsic cues prior to and/or after fertilization . How these cues are translated into developmental signals is poorly understood . Here through genetic screen...
Flowering plants are featured as double fertilization , a process that the egg cell and the central cell of embryo sac fuse with a sperm and give rise to a diploid zygote and a triploid primary endosperm cell , respectively . The zygote develops into embryo after cell division and differentiation , and starts a new tri...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "anatomy", "keratinocytes", "medicine", "and", "health", "sciences", "cell", "cycle", "and", "cell", "division", "cell", "processes", "ovules", "epithelial", "cells", "germ", "cells", "zygotes", "endosperm", "developmental", "biology", "luminescent", "protein...
2016
The Arabidopsis Receptor Kinase ZAR1 Is Required for Zygote Asymmetric Division and Its Daughter Cell Fate
Few models exist that accurately reproduce the complex rhythms of the thalamocortical system that are apparent in measured scalp EEG and at the same time , are suitable for large-scale simulations of brain activity . Here , we present a neural mass model of the thalamocortical system during natural non-REM sleep , whic...
Sleep plays a pivotal role for the consolidation of memory . While REM sleep had originally been the focus of research due to its similarity with wakefulness , more recent studies suggest that different sleep stages are responsible for the consolidation of different types of memory . To better understand the changes in...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "neurochemistry", "sleep", "depolarization", "membrane", "potential", "brain", "electrophysiology", "brain", "electrophysiology", "neuroscience", "learning", "and", "memory", "surgical", "and", "invasive", "medical", "procedures", ...
2016
A Thalamocortical Neural Mass Model of the EEG during NREM Sleep and Its Response to Auditory Stimulation
Distinct classes of neurons and glial cells in the developing spinal cord arise at specific times and in specific quantities from spatially discrete neural progenitor domains . Thus , adjacent domains can exhibit marked differences in their proliferative potential and timing of differentiation . However , remarkably li...
The embryonic spinal cord is organized into an array of discrete neural progenitor domains along the dorsoventral axis . Most of these domains undergo two periods of differentiation , first producing specific classes of neurons and then generating distinct populations of glial cells at later times . In addition , each ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
PLZF Regulates Fibroblast Growth Factor Responsiveness and Maintenance of Neural Progenitors
Cysteine proteinases of Fasciola hepatica are important candidates for vaccine antigens because of their role in fluke biology and host-parasite relationships . In our previous experiments , we found that a recombinant cysteine proteinase cloned from adult F . hepatica ( CPFhW ) can protect rats against liver fluke inf...
Infection with Fasciola hepatica , a liver fluke , is one of the most significant veterinary problems due to the worldwide distribution of this parasite , a wide spectrum of host organisms and the resulting economic loss . Human fasciolosis caused by F . hepatica is recognised by the World Health Organization as an imp...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "immune", "physiology", "chemical", "compounds", "enzymes", "immunology", "enzymology", "organic", "compounds", "vaccines", "preventive", "medicine", "amino", "acids", "infectious", "disease", "control", "antibodies", "plants", "...
2017
Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae
Experimental studies have shown that one key factor in driving the emergence of drug resistance in solid tumors is tumor hypoxia , which leads to the formation of localized environmental niches where drug-resistant cell populations can evolve and survive . Hypoxia-activated prodrugs ( HAPs ) are compounds designed to p...
It has been suggested that one key factor driving the emergence of drug resistance is the spatial heterogeneity in the distribution of drug and oxygen throughout a tumor due to disorganized tumor vasculatures . Researchers have developed a class of novel drugs that penetrate to hypoxic regions where they are activated ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "cell", "death", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "cardiovascular", "anatomy", "oxygen", "cancer", "treatment", "cell", "processes", "toxicology", "oncology", "toxicity", "pharmaceutics", "drug", ...
2016
Leveraging Hypoxia-Activated Prodrugs to Prevent Drug Resistance in Solid Tumors
Polyomavirus BK ( BKPyV ) frequently reactivates in immunosuppressed renal transplant recipients ( RTRs ) and may lead to graft loss due to BKPyV-induced interstitial nephritis ( BKVN ) . Little is known on the differentiation of CD8+ T cells targeting BKPyV in RTRs . Here we investigated whether BKPyV-specific CD8+ T ...
In immunosuppressed renal transplant recipients ( RTRs ) , BKPyV frequently reactivates from latency and may cause severe interstitial nephritis in the allograft ( BKVN ) . Not only is there no effective treatment , it also not understood why BKVN arises in some RTRs but not in all . In the current study we investigate...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "urinary", "system", "procedures", "medicine", "and", "health", "sciences", "innate", "immune", "system", "organ", "transplantation", "immune", "cells", "body", "fluids", "immune", "physiology", "cytokines", "viral", "transmission", "and", "infection...
2016
Clinically Relevant Reactivation of Polyomavirus BK (BKPyV) in HLA-A02-Positive Renal Transplant Recipients Is Associated with Impaired Effector-Memory Differentiation of BKPyV-Specific CD8+ T Cells
Intracellular pathogens have complex metabolic interactions with their host cells to ensure a steady supply of energy and anabolic building blocks for rapid growth . Here we use the obligate intracellular parasite Toxoplasma gondii to probe this interaction for isoprenoids , abundant lipidic compounds essential to many...
Toxoplasma gondii is an obligate intracellular parasite and is not able to replicate outside the host cell . The parasite lives in a specialized parasitophorous vacuole in contact with the host cytoplasm through the parasitophorous vacuole membrane . It is highly likely that a very active exchange of metabolites occurs...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Toxoplasma gondii Relies on Both Host and Parasite Isoprenoids and Can Be Rendered Sensitive to Atorvastatin
The biology of adult tsetse ( Glossina spp ) , vectors of trypanosomiasis in Africa , has been extensively studied – but little is known about larviposition in the field . In September-November 1998 , in the hot-dry season in Zimbabwe’s Zambezi Valley , we used artificial warthog burrows to capture adult females as the...
Adult tsetse , vectors of trypanosomiasis , have been extensively studied for more than 100 years , but little is known about larviposition behaviour in the field . Pupae are generally collected in the field via arduous searches of putative larviposition sites . Females have never been sampled in the field as they depo...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Artificial Warthog Burrows Used to Sample Adult and Immature Tsetse (Glossina spp) in the Zambezi Valley of Zimbabwe
Infection with filarial parasites is associated with T cell hyporesponsiveness , which is thought to be partly mediated by their ability to induce regulatory T cells ( Tregs ) during human infections . This study investigates the functional capacity of Tregs from different groups of filarial patients to suppress filari...
Lymphatic filariasis is a neglected disease still prominent in low-resource settings and is very disabling when it progresses to chronic pathology caused by lymphedema . Until now , studies on the contribution of Tregs to lymphocyte hyporesponsiveness in human filariasis have focused on frequency and phenotypic charact...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "immune", "cells", "cytokines", "immunity", "to", "infections", "immune", "suppression", "immunology", "adaptive", "immunity", "neglected", "tropical", "diseases", "immunoregulation", "immunomodulation", "lymphatic", "filariasis", "infectious", "diseases", "infl...
2012
Regulatory T Cells in Human Lymphatic Filariasis: Stronger Functional Activity in Microfilaremics
ChIP-seq enables genome-scale identification of regulatory regions that govern gene expression . However , the biological insights generated from ChIP-seq analysis have been limited to predictions of binding sites and cooperative interactions . Furthermore , ChIP-seq data often poorly correlate with in vitro measuremen...
A quantitative description of transcription factor ( TF ) binding in vivo is critical for our understanding of gene regulation . Chromatin Immunoprecipitation followed by sequencing ( ChIP-seq ) provides a genome-scale map of TF-binding . However , a quantitative characterization of the impact of genome accessibility o...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "bacteriology", "sequencing", "techniques", "engineering", "and", "technology", "gene", "regulation", "synthetic", "biology", "regulatory", "proteins", "microbiology", "dna-binding", "proteins", "invertebrate", "genomics", "transcription", "factors", "bacterial", "genetics", ...
2016
The Role of Genome Accessibility in Transcription Factor Binding in Bacteria
The motility and invasion of Plasmodium parasites is believed to require a cytoplasmic actin-myosin motor associated with a cell surface ligand belonging to the TRAP ( thrombospondin-related anonymous protein ) family . Current models of invasion usually invoke the existence of specific receptors for the TRAP-family li...
Apicomplexan parasites are one of the most significant groups of pathogens infecting humans and include Plasmodium falciparum , the parasite responsible for malaria . These parasites critically depend on their human host and must invade our cells to multiply; therefore , understanding this invasion process - with the e...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biomacromolecule-ligand", "interactions", "protein", "interactions", "microbiology", "host-pathogen", "interaction", "parasitology", "glycoproteins", "proteins", "cell", "adhesion", "extracellular", "matrix", "biology", "recombinant", "proteins", "proteomics", "biochemistry", ...
2012
Semaphorin-7A Is an Erythrocyte Receptor for P. falciparum Merozoite-Specific TRAP Homolog, MTRAP
Imaging studies have revealed a putative neural account of emotional bias in decision making . However , it has been difficult in previous studies to identify the causal role of the different sub-regions involved in decision making . The Ultimatum Game ( UG ) is a game to study the punishment of norm-violating behavior...
It is well-established that emotions influence decision making . One way of studying this relationship is the Ultimatum Game , which has revealed that subjects punish unfair behavior in others in spite of receiving a concomitant economic loss . Previous brain imaging studies have suggested that this decision to punish ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cognitive", "neuroscience", "behavioral", "neuroscience", "social", "and", "behavioral", "sciences", "psychology", "emotions", "cognition", "decision", "making", "behavior", "biology", "neuroscience" ]
2011
Limbic Justice—Amygdala Involvement in Immediate Rejection in the Ultimatum Game
Burkholderia pseudomallei is a soil-dwelling bacterium and the causative agent of melioidosis . The global burden and distribution of melioidosis is poorly understood , including in the Caribbean . B . pseudomallei was previously isolated from humans and soil in eastern Puerto Rico but the abundance and distribution of...
The objective of this study was to examine the distribution and abundance of Burkholderia pseudomallei in the environment in Puerto Rico . B . pseudomallei is a microbe that lives in soil and causes the disease melioidosis . We conducted sampling around Puerto Rico to survey for the presence of B . pseudomallei in the ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Disclaimer" ]
[ "taxonomy", "medicine", "and", "health", "sciences", "burkholderia", "pseudomallei", "pathology", "and", "laboratory", "medicine", "pathogens", "melioidosis", "geographical", "locations", "microbiology", "north", "america", "bacterial", "diseases", "burkholderia", "cepacia"...
2019
Burkholderia pseudomallei, the causative agent of melioidosis, is rare but ecologically established and widely dispersed in the environment in Puerto Rico
Cells of the embryonic vertebrate limb in high-density culture undergo chondrogenic pattern formation , which results in the production of regularly spaced “islands” of cartilage similar to the cartilage primordia of the developing limb skeleton . The first step in this process , in vitro and in vivo , is the generatio...
The development of an organism from embryo to adult includes processes of pattern formation that involve the interactions over space and time of independent cells to form multicellular structures . Computational models permit exploration of possible alternative mechanisms that reproduce biological patterns and thereby ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "chicken", "in", "vitro", "vertebrates", "computational", "biology" ]
2007
Patterns of Mesenchymal Condensation in a Multiscale, Discrete Stochastic Model
Although a considerable proportion of serum lipids loci identified in European ancestry individuals ( EA ) replicate in African Americans ( AA ) , interethnic differences in the distribution of serum lipids suggest that some genetic determinants differ by ethnicity . We conducted a comprehensive evaluation of five lipi...
Most of the work on the genetic epidemiology of serum lipids in African Americans ( AA ) has focused on replicating findings that were identified in European ancestry individuals . While this can be very informative about the generalizability of lipids loci across populations , African ancestry-specific variation will ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "biochemistry", "lipids", "epidemiology", "genetics", "biology", "human", "genetics", "genetic", "epidemiology" ]
2014
Gene-Based Sequencing Identifies Lipid-Influencing Variants with Ethnicity-Specific Effects in African Americans
Histone H3 lysine-4 ( H3K4 ) methylation is associated with transcribed genes in eukaryotes . In Drosophila and mammals , both di- and tri-methylation of H3K4 are associated with gene activation . In contrast to animals , in Arabidopsis H3K4 trimethylation , but not mono- or di-methylation of H3K4 , has been implicated...
Histones can be covalently modified and histone modifications regulate chromatin structure and gene transcription . One such modification is histone H3 lysine-4 ( H3K4 ) methylation , which can be mono- , di- , or tri-methylated . In animals such as fruitfly and mammals , both di- and tri-methylation of H3K4 are associ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "biology/plant", "growth", "and", "development", "plant", "biology/plant", "genetics", "and", "gene", "expression", "genetics", "and", "genomics/plant", "genetics", "and", "gene", "expression", "genetics", "and", "genomics/gene", "expression" ]
2011
Arabidopsis COMPASS-Like Complexes Mediate Histone H3 Lysine-4 Trimethylation to Control Floral Transition and Plant Development
Burkholderia pseudomallei , the causative agent of melioidosis , is an important public health threat due to limited therapeutic options for treatment . Efforts to improve therapeutics for B . pseudomallei infections are dependent on the need to understand the role of B . pseudomallei biofilm formation and its contribu...
B . pseudomallei , the etiological agent of melioidosis , is an emerging pathogen with limited therapeutic options and no available vaccines . A better understanding of the role of biofilm formation during pathogenesis will aid in melioidosis diagnosis and the development of new therapeutics and vaccines . Melioidosis ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biofilms", "exopolysaccharides", "gene", "regulation", "microbiology", "genetic", "elements", "research", "and", "analysis", "methods", "sequence", "analysis", "sequence", "alignment", "bioinformatics", "gene", "expression", "genetic", "loci", "biochemistry", "polysacchari...
2017
Genome-scale analysis of the genes that contribute to Burkholderia pseudomallei biofilm formation identifies a crucial exopolysaccharide biosynthesis gene cluster
In the zebrafish , Fgf and Hh signalling assign anterior and posterior identity , respectively , to the poles of the developing ear . Mis-expression of fgf3 or inhibition of Hh signalling results in double-anterior ears , including ectopic expression of hmx3a . To understand how this double-anterior pattern is establis...
Understanding how signalling molecules impart information to developing organ systems , and how this is interpreted through networks of gene activity , is a key goal of developmental genetic analysis . In the developing zebrafish inner ear , differences in gene expression arise between the anterior and posterior poles ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "fish", "otolith", "vesicles", "ears", "vertebrates", "animals", "animal", "models", "osteichthyes", "developmental", "biology", "inner", "ear", "model", "organisms", "experimental", "organism", "systems", "embryos", "cellular", ...
2019
Anteroposterior patterning of the zebrafish ear through Fgf- and Hh-dependent regulation of hmx3a expression
St . Louis encephalitis virus is a complex zoonoses . In 2005 , 47 laboratory-confirmed and probable clinical cases of SLEV infection were reported in Córdoba , Argentina . Although the causes of 2005 outbreak remain unknown , they might be related not only to virological factors , but also to ecological and environmen...
The St . Louis encephalitis is a complex zoonoses in the New World . In South America ( Argentina and Brazil ) , SLE is an emerging arbovirosis . SLEV reemerged in Argentina during 2002 and , since then , outbreaks have been reported in 2005 , 2006 , 2010 and 2011 . During the 2005 outbreak two SLEV genotype III strain...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "virology", "biology", "microbiology" ]
2012
Silent Circulation of St. Louis Encephalitis Virus Prior to an Encephalitis Outbreak in Cordoba, Argentina (2005)
In response to environmental and physiological changes , the synapse manifests plasticity while simultaneously maintains homeostasis . Here , we analyzed mutant synapses of henji , also known as dbo , at the Drosophila neuromuscular junction ( NMJ ) . In henji mutants , NMJ growth is defective with appearance of satell...
To meet various developmental or environmental needs , the communication between pre- and postsynapse can be modulated in different aspects . The release of presynaptic vesicles can be regulated at the steps of docking , membrane fusion and endocytosis . Upon receiving neurotransmitter stimuli from presynaptic terminal...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "rna", "interference", "nervous", "system", "cell", "processes", "electrophysiology", "neuroscience", "animals", "animal", "models", "drosophila", "melanogaster", "model", "organisms", "molecular", "biology", "tech...
2016
Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response
Plants have a large panel of nucleotide-binding/leucine rich repeat ( NLR ) immune receptors which monitor host interference by diverse pathogen molecules ( effectors ) and trigger disease resistance pathways . NLR receptor systems are necessarily under tight control to mitigate the trade-off between induced defenses a...
Plants tune their cellular and developmental programs to different environmental stimuli . Central players in the plant biotic stress response network are intracellular NLR receptors which intercept specific disease-inducing molecules ( effectors ) produced by pathogenic microbes . Variation in NLR gene repertoires bet...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "plant", "anatomy", "medicine", "and", "health", "sciences", "immunology", "brassica", "plant", "biotechnology", "plant", "science", "model", "organisms", "genetically", "modified", "plants", "plants", "extraction", "techniques", "genetic", "engineering",...
2016
Arabidopsis thaliana DM2h (R8) within the Landsberg RPP1-like Resistance Locus Underlies Three Different Cases of EDS1-Conditioned Autoimmunity
The phenotypic outcome of a mutation cannot be simply mapped onto the underlying DNA variant . Instead , the phenotype is a function of the allele , the genetic background in which it occurs and the environment where the mutational effects are expressed . While the influence of genetic background on the expressivity of...
Examining the consequences of how one mutation behaves when in the presence of a second mutation forms the basis of our understanding of genetic interactions , and is part of the fundamental toolbox of genetic analysis . Yet the logical interpretation of such mutational interactions depends on the generality of such fi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "epistasis", "mutation", "genetic", "mutation", "mutation", "types", "phenotypes", "genetic", "screens", "heredity", "genetics", "genetic", "suppression", "population", "genetics", "biology", "complex", "traits", "gene", "function" ]
2013
The Conditional Nature of Genetic Interactions: The Consequences of Wild-Type Backgrounds on Mutational Interactions in a Genome-Wide Modifier Screen
The discovery of small molecules targeted to specific oncogenic pathways has revolutionized anti-cancer therapy . However , such therapy often fails due to the evolution of acquired resistance . One long-standing question in clinical cancer research is the identification of optimum therapeutic administration strategies...
Recently , the field of anti-cancer therapy has witnessed a revolution by the discovery of targeted therapy , which refers to compounds targeting specific pathways causing abnormal growth of cancer cells . The clinical success of such drugs has been limited by the evolution of acquired resistance to these compounds , w...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "computational", "biology/evolutionary", "modeling", "mathematics", "oncology" ]
2009
Evolution of Resistance to Targeted Anti-Cancer Therapies during Continuous and Pulsed Administration Strategies
Cerebrospinal fluid ( CSF ) 42 amino acid species of amyloid beta ( Aβ42 ) and tau levels are strongly correlated with the presence of Alzheimer's disease ( AD ) neuropathology including amyloid plaques and neurodegeneration and have been successfully used as endophenotypes for genetic studies of AD . Additional CSF an...
The use of quantitative endophenotypes from cerebrospinal fluid has led to the identification of several genetic variants that alter risk or rate of progression of Alzheimer's disease . Here we have analyzed the levels of 58 disease-related proteins in the cerebrospinal fluid for association with millions of variants a...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genome-wide", "association", "studies", "quantitative", "trait", "association", "studies", "statistical", "analysis", "of", "genetic", "association", "inflammation", "medicine", "and", "health", "sciences", "genetic", "association", "studies", "genome", "analysis", "gene...
2014
Genome-Wide Association Study of CSF Levels of 59 Alzheimer's Disease Candidate Proteins: Significant Associations with Proteins Involved in Amyloid Processing and Inflammation
Exotic pathogens and pests threaten ecosystem service , biodiversity , and crop security globally . If an invasive agent can disperse asymptomatically over long distances , multiple spatial and temporal scales interplay , making identification of effective strategies to regulate , monitor , and control disease extremel...
We discuss principles governing the spread and management of diseases in natural forest ecosystems . Invasive organisms are damaging world forests and agricultural crops at an increasing rate and severity due to global trade and environmental disturbances . While prevention is the best option , practitioners must decid...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "public", "health", "and", "epidemiology", "forestry", "infectious", "disease", "epidemiology", "plant", "biology", "statistics", "population", "dynamics", "spatial", "epidemiology", "integrated", "control", "plant", "science", "mathematics", "pest", "control", "plant", ...
2012
Landscape Epidemiology and Control of Pathogens with Cryptic and Long-Distance Dispersal: Sudden Oak Death in Northern Californian Forests
The brassinosteroids ( BRs ) represent a class of phytohormones , which regulate numerous aspects of growth and development . Here , a det2-9 mutant defective in BR synthesis was identified from an EMS mutant screening for defects in root length , and was used to investigate the role of BR in root development in Arabid...
Both brassinosteroids ( BRs ) and ethylene have been known to control root growth and development . ROS have been also reported to play an important role in root development . However , the relationship between BRs and ethylene or ROS in root growth and development was not addressed before . In this study , a det2-9 mu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "peroxidases", "chemical", "compounds", "plant", "growth", "and", "development", "ethylene", "signaling", "cascade", "ethylene", "enzymes", "brassica", "enzymology", "organic", "compounds", "hormones", "developmental", "biology", "plant", "science", "model", "organisms", ...
2018
Brassinosteroids regulate root growth by controlling reactive oxygen species homeostasis and dual effect on ethylene synthesis in Arabidopsis
The community , the assemblage of organisms co-existing in a given space and time , has the potential to become one of the unifying concepts of biology , especially with the advent of high-throughput sequencing experiments that reveal genetic diversity exhaustively . In this spirit we show that a tool from community ec...
Living things are parts of complex communities , similar to humans living in cities . A quantitative way of describing such communities is to measure the abundance of each species in the community so that a sorted list of abundance numbers is produced , a so-called Rank Abundance Distribution ( RAD ) . With recent brea...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "taxonomy", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "immune", "cells", "microbiome", "immunology", "microbiology", "data", "management", "b", "cell", "receptors", "memory", "b", "cells", "thermodynamics...
2017
Quantitative Comparison of Abundance Structures of Generalized Communities: From B-Cell Receptor Repertoires to Microbiomes
The covalently closed circular DNA ( cccDNA ) of the hepatitis B virus ( HBV ) plays an essential role in chronic hepatitis . The cellular repair system is proposed to convert cytoplasmic nucleocapsid ( NC ) DNA ( partially double-stranded DNA ) into cccDNA in the nucleus . Recently , antiviral cytidine deaminases , AI...
Human cytidine deaminases , AID/APOBECs , are restriction factors against various types of viruses . These proteins have the ability to introduce a cytidine-to-uridine ( C-to-U ) hypermutation in the viral DNAs of the hepadnaviruses hepatitis B virus ( HBV ) and duck HBV ( DHBV ) models . It is well known that uracil r...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "hepatitis", "hepatitis", "b", "dna", "modification", "nucleic", "acids", "dna", "viruses", "viral", "classification", "virology", "dna", "dna", "repair", "biology", "microbiology", "molecular", "cell", "biology", "viral", "diseas...
2013
Uracil DNA Glycosylase Counteracts APOBEC3G-Induced Hypermutation of Hepatitis B Viral Genomes: Excision Repair of Covalently Closed Circular DNA
Visual estimation of the material and shape of an object from a single image includes a hard ill-posed computational problem . However , in our daily life we feel we can estimate both reasonably well . The neural computation underlying this ability remains poorly understood . Here we propose that the human visual syste...
Objects in our visual world contain a variety of material information . Although such information enables us to experience rich material impressions , it can be a distraction for the estimation of other physical properties such as shapes , albedos , and illuminations . The coupled estimation of these properties we huma...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "classical", "mechanics", "atmospheric", "science", "astronomical", "sciences", "social", "sciences", "light", "neuroscience", "electromagnetic", "radiation", "probability", "distribution", "luminance", "mathematics", "reflection", "...
2018
Material and shape perception based on two types of intensity gradient information
The chronic phase of HIV infection is marked by pathological activation of the immune system , the extent of which better predicts disease progression than either plasma viral load or CD4+ T cell count . Recently , translocation of microbial products from the gastrointestinal tract has been proposed as an underlying ca...
Persistent activation of the immune system is a hallmark of chronic HIV/SIV infections and predicts disease progression better than either plasma viral load or CD4+ T cell count . While the causes of immune activation during chronic infection are likely multifactorial , recent work has shown that microbial translocatio...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/hiv", "infection", "and", "aids" ]
2010
Damaged Intestinal Epithelial Integrity Linked to Microbial Translocation in Pathogenic Simian Immunodeficiency Virus Infections
The highly variable tprK gene of Treponema pallidum has been acknowledged to be one of the mechanisms that causes persistent infection . Previous studies have mainly focused on the heterogeneity in tprK in propagated strains using a clone-based Sanger approach . Few studies have investigated tprK directly from clinical...
Variations in tprK have been acknowledged to be the major contributors to persistent Treponema pallidum infections . Previous studies were based on the clone-based Sanger approach , and most of them were performed in propagated strains using rabbits , which could not reflect the actual heterogeneous characteristics of ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "sequencing", "techniques", "urology", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "population", "genetics", "tropical", "diseases", "microbiology", "treponematoses", "bacterial", "diseases", "next-generation", "sequen...
2019
Profile of the tprK gene in primary syphilis patients based on next-generation sequencing
Gut microbiota are shaped by a combination of ecological and evolutionary forces . While the ecological dynamics have been extensively studied , much less is known about how species of gut bacteria evolve over time . Here , we introduce a model-based framework for quantifying evolutionary dynamics within and across hos...
The human gut harbors a diverse microbial community whose composition is shaped by a variety of ecological forces . Given the high rates of turnover , the residents of this community might also have the opportunity to evolve over time by acquiring heritable changes to their genomes . Yet , despite the potential importa...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "bacteriology", "organismal", "evolution", "gut", "bacteria", "population", "genetics", "microbiology", "twins", "developmental", "biology", "metagenomics", "microbial", "evolution", "evolutionary", "adaptation", "population", "biology", "bacteria", "evolutionary", "genetics"...
2019
Evolutionary dynamics of bacteria in the gut microbiome within and across hosts
Studies of nervous system connectivity , in a wide variety of species and at different scales of resolution , have identified several highly conserved motifs of network organization . One such motif is a heterogeneous distribution of connectivity across neural elements , such that some elements act as highly connected ...
Some elements of neural systems possess many more connections than others , marking them as network hubs . These hubs are often densely interconnected with each other , forming a so-called rich-club that is thought to support integrated function . Recent work in the mouse suggests that connected pairs of hubs show high...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "neural", "networks", "caenorhabditis", "nervous", "system", "electrophysiology", "neuroscience", "animals", "motor", "neurons", "animal", "models", "caenorhabditis", "elegans", "model", "organisms", "brain", "mapp...
2018
Hub connectivity, neuronal diversity, and gene expression in the Caenorhabditis elegans connectome
Cyclic AMP-dependent pathways mediate the communication between external stimuli and the intracellular signaling machinery , thereby influencing important aspects of cellular growth , morphogenesis and differentiation . Crucial to proper function and robustness of these signaling cascades is the strict regulation and m...
Magnaporthe oryzae , an economically important fungal pathogen that causes the blast disease in rice and several cereal crops , is a model organism for studying fungus-host interactions . M . oryzae reproduces asexually by producing spores , which can switch to an infectious disease-causing mode of development in respo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/microbial", "growth", "and", "development", "genetics", "and", "genomics/gene", "function", "microbiology/plant-biotic", "interactions", "cell", "biology/cell", "signaling" ]
2010
PdeH, a High-Affinity cAMP Phosphodiesterase, Is a Key Regulator of Asexual and Pathogenic Differentiation in Magnaporthe oryzae
Mechanisms generating diverse cell types from multipotent progenitors are fundamental for normal development . Pigment cells are derived from multipotent neural crest cells and their diversity in teleosts provides an excellent model for studying mechanisms controlling fate specification of distinct cell types . Zebrafi...
How individual cell fates become specified from multipotent progenitors is a fundamental question in developmental and stem cell biology . Body pigment cells derive from a multipotent progenitor , but while in zebrafish there are three types of pigment cells ( melanocytes , iridophores and xanthophores ) , in medaka th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "fish", "medicine", "and", "health", "sciences", "vertebrates", "pigments", "animals", "neuroscience", "alleles", "epithelial", "cells", "animal", "models", "osteichthyes", "developmental", "biology", "model", "organisms", "stem", "cells", "materials", "science", "exper...
2018
Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish