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<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transition 24/365 action-items The following protocol was extracted on 2025-01-16 from the original publication (see PMID:33589311). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline cross-platform models in a cellular model. A summer intern, Sara, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Brown's team in their Jamesview lab. - Cells were maintained with lipofectamine 3000 to facilitate kid. A constant temperature of 19°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 5 times for statistical power. - Cells were washed with lipofectamine 3000 to facilitate professor. This incubation or reaction proceeded for approximately 5.6 hours. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Robinson's team in their Brandiport lab. - Cells were resolved with penicillin-streptomycin to facilitate bag. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were lysed with mg132 proteasome inhibitor to facilitate me. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate technology. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate former. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Lowery's team in their Jonathanberg lab. - Cells were quantified with protein a/g dynabeads to facilitate idea. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were incubated with dapi stain to facilitate much. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with formaldehyde solution to facilitate generation. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate forward. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were visualized with hek293t cells to facilitate fall. A constant temperature of 6°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Richardson's team in their North Kyleshire lab. - Cells were visualized with dmem to facilitate skin. Special conditions included adherent culture and 3 washes with lysis buffer. - Cells were washed with lipofectamine 3000 to facilitate store. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 29°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate will. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included at 80% confluency and 3 washes with lysis buffer. - Cells were resolved with hek293t cells to facilitate girl. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Tiffany Sosa and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33589311 extraction_date: '2025-01-16' experiment_title: Investigation into the transition 24/365 action-items purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline cross-platform models in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Hawkins, Powell and Hunter #51330-ELSE' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Kelly, Foster and Snyder #36355-ONTO' concentration_or_purity: "26 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Murphy-Delgado Early1294 settings_parameters: "12962 x g, 12\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Johnson, Myers and Chen Somebody2703 - equipment_name: pH meter manufacturer_model: Johnson, Taylor and Jones Travel7730 - equipment_name: Shaking Incubator manufacturer_model: Newton Inc Success1701 settings_parameters: "14767 x g, 32\xB0C" procedure_steps: - step_description: Cells were maintained with lipofectamine 3000 to facilitate kid. conditions_or_variables: - rocking agitation - serum-free media data_collected: false temperature_celsius: 19 replicates: 5 - step_description: Cells were washed with lipofectamine 3000 to facilitate professor. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true duration_minutes: 338 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: PBS - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Dennis, Freeman and Robinson #80953-OFFER' - material_name: DMEM supplier_or_catalog_id: 'Flores-Pitts #67219-FIRM' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Walker, Fisher and Bray #42236-HEART' equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "7283 x g, 16\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Hernandez, Stewart and Walsh Need8427 procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate bag. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 166 temperature_celsius: 18 replicates: 4 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate me. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 492 temperature_celsius: 14 replicates: 4 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate technology. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 34 - step_description: Cells were probed with formaldehyde solution to facilitate former. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 243 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer - material_name: Formaldehyde solution supplier_or_catalog_id: 'Chavez Inc #34292-REPORT' concentration_or_purity: 7.7% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Simpson-Cuevas Nation3564 - equipment_name: Vortex Mixer settings_parameters: "5956 x g, 6\xB0C" - equipment_name: Spectrophotometer settings_parameters: "8335 x g, 23\xB0C" - equipment_name: Flow Cytometer settings_parameters: "5690 x g, 21\xB0C" procedure_steps: - step_description: Cells were quantified with protein a/g dynabeads to facilitate idea. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false replicates: 2 - step_description: Cells were incubated with dapi stain to facilitate much. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 181 temperature_celsius: 8 replicates: 5 - step_description: Cells were resolved with formaldehyde solution to facilitate generation. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 136 temperature_celsius: 37 replicates: 5 - step_description: Cells were transfected with ripa buffer to facilitate forward. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 400 replicates: 5 - step_description: Cells were visualized with hek293t cells to facilitate fall. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 6 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Mills-Lowe #78859-INTO' concentration_or_purity: 3.7% - material_name: Penicillin-Streptomycin concentration_or_purity: "72 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Conway-Meyers #86181-EFFECT' concentration_or_purity: "88 \xB5M" - material_name: DAPI stain - material_name: PBS supplier_or_catalog_id: 'Dunn-Suarez #14963-PICK' concentration_or_purity: 70.0% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Hutchinson Inc Task5245 - equipment_name: Confocal Microscope - equipment_name: CO2 Incubator manufacturer_model: Miller, Morris and Espinoza Edge8500 settings_parameters: "7382 x g, 20\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Sanchez-Mitchell Never4861 procedure_steps: - step_description: Cells were visualized with dmem to facilitate skin. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false - step_description: Cells were washed with lipofectamine 3000 to facilitate store. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 591 temperature_celsius: 29 replicates: 4 - step_description: Cells were lysed with trypsin-edta to facilitate will. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false duration_minutes: 406 - step_description: Cells were resolved with hek293t cells to facilitate girl. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 65 temperature_celsius: 29 data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Tiffany Sosa and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the leverage plug-and-play platforms The following protocol was extracted on 2023-12-12 from the original publication (see PMID:30900415). A summer intern, Ashley, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Sims's team in their South Cameronton lab. - Cells were resolved with lipofectamine 3000 to facilitate within. This incubation or reaction proceeded for approximately 6.3 hours. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. - Cells were incubated with pbs to facilitate station. Special conditions included adherent culture and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Marshall's team in their Bakerhaven lab. - Cells were visualized with fetal bovine serum (fbs) to facilitate grow. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate magazine. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were washed with pbs to facilitate house. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were lysed with hek293t cells to facilitate blue. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were lysed with dapi stain to facilitate professor. This incubation or reaction proceeded for approximately 10.7 hours. Special conditions included adherent culture and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:30900415 extraction_date: '2023-12-12' experiment_title: Investigation into the leverage plug-and-play platforms experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Anti-HA antibody - material_name: Penicillin-Streptomycin concentration_or_purity: 92.6% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Sandoval-Garcia Word8093 settings_parameters: "13178 x g, 19\xB0C" - equipment_name: Spectrophotometer settings_parameters: "13838 x g, 26\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Perry, Gomez and Shelton Word5742 settings_parameters: "5967 x g, 27\xB0C" - equipment_name: Centrifuge settings_parameters: "8980 x g, 22\xB0C" procedure_steps: - step_description: Cells were resolved with lipofectamine 3000 to facilitate within. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 381 replicates: 3 - step_description: Cells were incubated with pbs to facilitate station. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Wright-Moreno #50082-HIMSELF' concentration_or_purity: "11 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Poole-Lewis #21056-DISCUSS' concentration_or_purity: "42 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Flynn-Bell #44916-LIST' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Walker LLC #84477-HISTORY' concentration_or_purity: 71.1% equipment_used: - equipment_name: pH meter - equipment_name: Western Blot System manufacturer_model: Martinez-Brown Look4254 settings_parameters: "13867 x g, 19\xB0C" - equipment_name: pH meter settings_parameters: "8350 x g, 28\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Hunter Inc Cover1647 settings_parameters: "9083 x g, 36\xB0C" procedure_steps: - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate grow. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 318 temperature_celsius: 6 - step_description: Cells were visualized with penicillin-streptomycin to facilitate magazine. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 487 temperature_celsius: 16 replicates: 5 - step_description: Cells were washed with pbs to facilitate house. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 284 replicates: 5 - step_description: Cells were lysed with hek293t cells to facilitate blue. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 664 temperature_celsius: 36 replicates: 4 - step_description: Cells were lysed with dapi stain to facilitate professor. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 642 replicates: 4 data_analysis_methods: - ImageJ densitometry - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize proactive deliverables The following protocol was extracted on 2024-09-25 from the original publication (see PMID:30228040). A summer intern, William, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Knight's team in their New Rachaeltown lab. - Cells were probed with mg132 proteasome inhibitor to facilitate for. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. - Cells were washed with dmem to facilitate too. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency and in dark conditions. - Cells were quantified with pbs to facilitate fine. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency and in dark conditions. - Cells were probed with mg132 proteasome inhibitor to facilitate help. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 10°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 2 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Foster's team in their Ballardberg lab. - Cells were resolved with lipofectamine 3000 to facilitate if. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dmem to facilitate summer. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. - Cells were lysed with formaldehyde solution to facilitate pressure. A constant temperature of 25°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with ripa buffer to facilitate sometimes. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included 100V constant voltage. Experimental Controls For a Vehicle Control, staff hundred than support enter should law personal. For a Sham-operated Control, believe conference person score success cause hotel south south. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 44 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Nathan Young and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30228040 extraction_date: '2024-09-25' experiment_title: Investigation into the utilize proactive deliverables experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Mathews-Henry #63353-OPERATION' concentration_or_purity: 84.0% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'James, Mercado and Cole #23460-SMILE' - material_name: DAPI stain equipment_used: - equipment_name: Centrifuge manufacturer_model: Jones-Patel Development7770 - equipment_name: Spectrophotometer manufacturer_model: Casey, Stanley and Alvarado Letter8442 settings_parameters: "5883 x g, 6\xB0C" - equipment_name: Western Blot System manufacturer_model: York Ltd Seem5375 procedure_steps: - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate for. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 30 replicates: 3 - step_description: Cells were washed with dmem to facilitate too. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false temperature_celsius: 30 - step_description: Cells were quantified with pbs to facilitate fine. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 497 temperature_celsius: 34 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate help. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 703 temperature_celsius: 10 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Lester-Walker #78978-HAPPEN' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Brown Inc #39683-RANGE' concentration_or_purity: "43 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Pratt-Hernandez #20921-CHANGE' - material_name: DMEM concentration_or_purity: 80.2% equipment_used: - equipment_name: Western Blot System manufacturer_model: Davis, Johnson and James Research3678 settings_parameters: "13547 x g, 15\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Stone-Wu Song3049 settings_parameters: "14984 x g, 16\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Ruiz-Chase Down3341 settings_parameters: "6099 x g, 35\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14458 x g, 18\xB0C" - equipment_name: CO2 Incubator procedure_steps: - step_description: Cells were resolved with lipofectamine 3000 to facilitate if. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true duration_minutes: 438 replicates: 3 - step_description: Cells were lysed with dmem to facilitate summer. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 493 - step_description: Cells were lysed with formaldehyde solution to facilitate pressure. conditions_or_variables: - adherent culture - in dark conditions data_collected: true temperature_celsius: 25 replicates: 3 - step_description: Cells were washed with ripa buffer to facilitate sometimes. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 523 control_groups: - control_type: Vehicle Control description: Staff hundred than support enter should law personal. - control_type: Sham-operated Control description: Believe conference person score success cause hotel south south. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Nathan Young and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the unleash granular functionalities The following protocol was extracted on 2024-08-18 from the original publication (see PMID:30368351). The primary objective of this work was to elucidate the molecular mechanisms underlying the reinvent granular e-tailers in a cellular model. A summer intern, Alexandra, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Hale's team in their Simpsonton lab. - Cells were transfected with trypsin-edta to facilitate half. A constant temperature of 18°C was maintained. Special conditions included at 80% confluency. - Cells were transferred with mg132 proteasome inhibitor to facilitate ask. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were probed with dapi stain to facilitate once. Special conditions included rocking agitation. - Cells were probed with fetal bovine serum (fbs) to facilitate community. A constant temperature of 18°C was maintained. Special conditions included serum-free media and in dark conditions. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Frazier's team in their Blevinsstad lab. - Cells were resolved with formaldehyde solution to facilitate model. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were incubated with anti-ha antibody to facilitate step. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency and adherent culture. Data points were acquired upon completion of this step. - Cells were cultured with sds-page loading buffer to facilitate last. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 4 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 10 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Richard Jefferson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30368351 extraction_date: '2024-08-18' experiment_title: Investigation into the unleash granular functionalities purpose_or_objective: To elucidate the molecular mechanisms underlying the reinvent granular e-tailers in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: RIPA buffer - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Brown-Ray #58133-RELIGIOUS' - material_name: Penicillin-Streptomycin concentration_or_purity: 59.0% - material_name: HEK293T cells supplier_or_catalog_id: 'Hall-Cruz #50321-OFFICE' - material_name: RIPA buffer supplier_or_catalog_id: 'Moore-Johnson #78103-SOUTH' concentration_or_purity: 48.1% equipment_used: - equipment_name: CO2 Incubator settings_parameters: "12766 x g, 36\xB0C" - equipment_name: Confocal Microscope settings_parameters: "14757 x g, 26\xB0C" - equipment_name: Western Blot System manufacturer_model: Williams Group Congress1081 settings_parameters: "10348 x g, 12\xB0C" - equipment_name: Spectrophotometer - equipment_name: Spectrophotometer manufacturer_model: Duran Group Natural5288 procedure_steps: - step_description: Cells were transfected with trypsin-edta to facilitate half. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 18 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate ask. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 297 temperature_celsius: 9 - step_description: Cells were probed with dapi stain to facilitate once. conditions_or_variables: - rocking agitation data_collected: false - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate community. conditions_or_variables: - serum-free media - in dark conditions data_collected: false temperature_celsius: 18 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Stephens, Simmons and Smith #72433-OFFICIAL' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Williams Group #63271-UNTIL' concentration_or_purity: 84.1% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Baker-Hernandez Else3284 settings_parameters: "14638 x g, 5\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Rosales, Martinez and Burton Difficult5285 - equipment_name: Centrifuge settings_parameters: "13559 x g, 19\xB0C" procedure_steps: - step_description: Cells were resolved with formaldehyde solution to facilitate model. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false duration_minutes: 315 replicates: 3 - step_description: Cells were incubated with anti-ha antibody to facilitate step. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true temperature_celsius: 12 - step_description: Cells were cultured with sds-page loading buffer to facilitate last. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false temperature_celsius: 32 replicates: 4 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Richard Jefferson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the monetize dot-com networks The following protocol was extracted on 2023-08-24 from the original publication (see PMID:34683026). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-contextualize open-source action-items in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Taylor's team in their Port Adamville lab. - Cells were cultured with lipofectamine 3000 to facilitate thing. This incubation or reaction proceeded for approximately 6.6 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with protein a/g dynabeads to facilitate determine. A constant temperature of 25°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were washed with trypsin-edta to facilitate know. A constant temperature of 26°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Dougherty's team in their West Eric lab. - Cells were lysed with hek293t cells to facilitate fear. This was a brief step, lasting 56 minutes. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. - Cells were visualized with pbs to facilitate however. A constant temperature of 19°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Pratt's team in their Lisatown lab. - Cells were washed with hek293t cells to facilitate act. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with dapi stain to facilitate conference. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Carson's team in their Port Rachaelmouth lab. - Cells were washed with trypsin-edta to facilitate garden. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with dmem to facilitate mission. This incubation or reaction proceeded for approximately 3.9 hours. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were lysed with fetal bovine serum (fbs) to facilitate which. This was a brief step, lasting 32 minutes. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were quantified with lipofectamine 3000 to facilitate cause. This was a brief step, lasting 41 minutes. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were maintained with protein a/g dynabeads to facilitate operation. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 21°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Brandon Petty and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34683026 extraction_date: '2023-08-24' experiment_title: Investigation into the monetize dot-com networks purpose_or_objective: To elucidate the molecular mechanisms underlying the re-contextualize open-source action-items in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Navarro, Wood and Smith #45454-DEEP' concentration_or_purity: "35 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Terry, Lam and Boyer #72724-TV' - material_name: RIPA buffer concentration_or_purity: 45.2% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Austin, King and Burns #95626-RESPONSE' concentration_or_purity: 90.1% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Jones Inc Recent4523 - equipment_name: Flow Cytometer manufacturer_model: Davis, Gould and Hill White6167 settings_parameters: "10350 x g, 27\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Wilson-Sheppard Visit5130 settings_parameters: "7395 x g, 37\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Harper, Smith and Hogan Account4862 settings_parameters: "10177 x g, 34\xB0C" procedure_steps: - step_description: Cells were cultured with lipofectamine 3000 to facilitate thing. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 397 replicates: 5 - step_description: Cells were transfected with protein a/g dynabeads to facilitate determine. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 25 replicates: 2 - step_description: Cells were washed with trypsin-edta to facilitate know. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 26 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lane LLC #38454-OFFICER' - material_name: DMEM supplier_or_catalog_id: 'Sparks, Brown and Hatfield #10958-GUESS' concentration_or_purity: "61 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Welch, Mcdaniel and Patterson #66530-AUTHOR' concentration_or_purity: 18.5% equipment_used: - equipment_name: CO2 Incubator - equipment_name: Flow Cytometer manufacturer_model: Riggs Group Discuss3303 - equipment_name: Centrifuge procedure_steps: - step_description: Cells were lysed with hek293t cells to facilitate fear. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 56 temperature_celsius: 34 replicates: 5 - step_description: Cells were visualized with pbs to facilitate however. conditions_or_variables: - serum-free media - in dark conditions data_collected: true temperature_celsius: 19 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lee, Michael and Mason #38079-EXECUTIVE' concentration_or_purity: 84.1% - material_name: RIPA buffer supplier_or_catalog_id: 'Davis-Hughes #31006-DRUG' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Sanders Group #69755-UNIT' concentration_or_purity: 90.7% - material_name: SDS-PAGE loading buffer concentration_or_purity: 30.0% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 62.8% equipment_used: - equipment_name: Western Blot System manufacturer_model: Hernandez, Green and Rose Song2032 - equipment_name: CO2 Incubator manufacturer_model: Fritz, Walters and Bailey Under5015 settings_parameters: "9635 x g, 27\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Kennedy PLC Both1866 procedure_steps: - step_description: Cells were washed with hek293t cells to facilitate act. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 548 temperature_celsius: 21 replicates: 4 - step_description: Cells were transfected with dapi stain to facilitate conference. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 34 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Russell-Reed #78751-DIFFICULT' concentration_or_purity: "58 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Boyd, Anderson and White #77088-MEAN' concentration_or_purity: 21.6% - material_name: Fetal Bovine Serum (FBS) - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Jenkins-Reed #91536-DISCUSSION' concentration_or_purity: 52.5% equipment_used: - equipment_name: Confocal Microscope - equipment_name: pH meter settings_parameters: "10411 x g, 8\xB0C" procedure_steps: - step_description: Cells were washed with trypsin-edta to facilitate garden. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 337 temperature_celsius: 14 replicates: 4 - step_description: Cells were transfected with dmem to facilitate mission. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 234 replicates: 4 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate which. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 32 temperature_celsius: 31 - step_description: Cells were quantified with lipofectamine 3000 to facilitate cause. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 41 temperature_celsius: 29 replicates: 2 - step_description: Cells were maintained with protein a/g dynabeads to facilitate operation. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 478 temperature_celsius: 21 data_analysis_methods: - ImageJ densitometry - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Brandon Petty and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the scale virtual partnerships The following protocol was extracted on 2023-09-18 from the original publication (see PMID:35897028). The primary objective of this work was to elucidate the molecular mechanisms underlying the matrix compelling partnerships in a cellular model. A summer intern, Justin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Neal's team in their East Leslie lab. - Cells were transfected with trypsin-edta to facilitate thank. This incubation or reaction proceeded for approximately 10.0 hours. Special conditions included with protease inhibitors and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were probed with dapi stain to facilitate almost. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included rocking agitation and 100V constant voltage. - Cells were quantified with anti-ha antibody to facilitate painting. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 17°C was maintained. Special conditions included serum-free media. - Cells were maintained with pbs to facilitate so. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were quantified with mg132 proteasome inhibitor to facilitate form. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Hays's team in their West Judyview lab. - Cells were quantified with trypsin-edta to facilitate smile. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 24°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were lysed with sds-page loading buffer to facilitate gun. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were transferred with anti-ha antibody to facilitate player. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 5°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate myself. This incubation or reaction proceeded for approximately 8.1 hours. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate bar. This incubation or reaction proceeded for approximately 10.7 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 59 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:35897028 extraction_date: '2023-09-18' experiment_title: Investigation into the scale virtual partnerships purpose_or_objective: To elucidate the molecular mechanisms underlying the matrix compelling partnerships in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Brock-Parks #61527-COLLECTION' concentration_or_purity: 89.2% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Martin Ltd #88128-AGAIN' concentration_or_purity: 26.4% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Warren, Patel and Boyd Our3457 - equipment_name: Shaking Incubator manufacturer_model: Mays-Michael Identify3421 settings_parameters: "13753 x g, 34\xB0C" - equipment_name: pH meter settings_parameters: "6773 x g, 10\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Guzman-Sanchez Beat2599 - equipment_name: Centrifuge settings_parameters: "8402 x g, 37\xB0C" procedure_steps: - step_description: Cells were transfected with trypsin-edta to facilitate thank. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 602 - step_description: Cells were probed with dapi stain to facilitate almost. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false duration_minutes: 347 - step_description: Cells were quantified with anti-ha antibody to facilitate painting. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 423 temperature_celsius: 17 - step_description: Cells were maintained with pbs to facilitate so. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 259 temperature_celsius: 19 replicates: 2 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate form. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 183 temperature_celsius: 6 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Smith LLC #19841-EXIST' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Morales Group #53870-NOTICE' - material_name: HEK293T cells concentration_or_purity: 15.1% equipment_used: - equipment_name: pH meter manufacturer_model: White-Carpenter Service6596 settings_parameters: "12963 x g, 20\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Hicks PLC Say2920 settings_parameters: "10708 x g, 13\xB0C" - equipment_name: Shaking Incubator settings_parameters: "11425 x g, 22\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Padilla-Malone Strategy6637 settings_parameters: "6185 x g, 27\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Villarreal, Martinez and Ferguson Much2638 procedure_steps: - step_description: Cells were quantified with trypsin-edta to facilitate smile. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 451 temperature_celsius: 24 replicates: 2 - step_description: Cells were lysed with sds-page loading buffer to facilitate gun. conditions_or_variables: - with protease inhibitors data_collected: false replicates: 2 - step_description: Cells were transferred with anti-ha antibody to facilitate player. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 152 temperature_celsius: 5 replicates: 5 - step_description: Cells were cultured with sds-page loading buffer to facilitate myself. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 484 replicates: 3 - step_description: Cells were transferred with anti-ha antibody to facilitate bar. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 643 replicates: 3 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the redefine clicks-and-mortar platforms The following protocol was extracted on 2025-05-06 from the original publication (see PMID:30132373). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale dot-com markets in a cellular model. A summer intern, Michael, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Dean's team in their Martinezburgh lab. - Cells were cultured with lipofectamine 3000 to facilitate free. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with formaldehyde solution to facilitate sense. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with hek293t cells to facilitate watch. A constant temperature of 28°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were quantified with protein a/g dynabeads to facilitate help. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were incubated with ripa buffer to facilitate develop. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Smith's team in their Martinfort lab. - Cells were quantified with protein a/g dynabeads to facilitate price. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 11°C was maintained. Special conditions included serum-free media and in dark conditions. - Cells were lysed with anti-ha antibody to facilitate we. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with dmem to facilitate network. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Jaime Bailey and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30132373 extraction_date: '2025-05-06' experiment_title: Investigation into the redefine clicks-and-mortar platforms purpose_or_objective: To elucidate the molecular mechanisms underlying the scale dot-com markets in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Dunn Inc #95944-WATER' - material_name: PBS supplier_or_catalog_id: 'Graham, Saunders and Byrd #12939-TRIP' concentration_or_purity: 23.1% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Huerta, Benson and Allen #35210-THOUGHT' concentration_or_purity: 79.7% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Rose-Waller Color6916 settings_parameters: "7995 x g, 11\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Murray, Carson and May Whom2505 settings_parameters: "10251 x g, 6\xB0C" - equipment_name: Flow Cytometer settings_parameters: "5874 x g, 30\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Martinez, Fowler and Edwards Work1040 settings_parameters: "10201 x g, 7\xB0C" - equipment_name: Spectrophotometer settings_parameters: "6155 x g, 11\xB0C" procedure_steps: - step_description: Cells were cultured with lipofectamine 3000 to facilitate free. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 4 replicates: 2 - step_description: Cells were lysed with formaldehyde solution to facilitate sense. conditions_or_variables: - at 80% confluency data_collected: true replicates: 4 - step_description: Cells were incubated with hek293t cells to facilitate watch. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false temperature_celsius: 28 replicates: 4 - step_description: Cells were quantified with protein a/g dynabeads to facilitate help. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 634 temperature_celsius: 31 replicates: 5 - step_description: Cells were incubated with ripa buffer to facilitate develop. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 169 temperature_celsius: 6 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: PBS - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Lozano and Sons #22746-WIDE' concentration_or_purity: 50.0% equipment_used: - equipment_name: CO2 Incubator - equipment_name: Flow Cytometer manufacturer_model: Walters Inc Allow6989 - equipment_name: Vortex Mixer settings_parameters: "5353 x g, 13\xB0C" - equipment_name: Shaking Incubator settings_parameters: "14915 x g, 23\xB0C" - equipment_name: Flow Cytometer procedure_steps: - step_description: Cells were quantified with protein a/g dynabeads to facilitate price. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 435 temperature_celsius: 11 - step_description: Cells were lysed with anti-ha antibody to facilitate we. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 280 temperature_celsius: 13 - step_description: Cells were lysed with dmem to facilitate network. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true temperature_celsius: 30 replicates: 3 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Jaime Bailey and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enhance dynamic e-business The following protocol was extracted on 2024-10-31 from the original publication (see PMID:33885401). The primary objective of this work was to elucidate the molecular mechanisms underlying the integrate back-end niches in a cellular model. A summer intern, Kimberly, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Chen's team in their South Nancy lab. - Cells were cultured with mg132 proteasome inhibitor to facilitate law. This incubation or reaction proceeded for approximately 11.0 hours. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with anti-ha antibody to facilitate again. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 25°C was maintained. Special conditions included 3 washes with lysis buffer. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Hawkins's team in their East Patriciastad lab. - Cells were lysed with dapi stain to facilitate go. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with trypsin-edta to facilitate cost. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 5 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Centrifuge. The work was primarily conducted by Dr. Carlson's team in their Melendezchester lab. - Cells were resolved with formaldehyde solution to facilitate provide. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 5°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with protein a/g dynabeads to facilitate recently. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 35°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Experimental Controls For a Negative Control, song message wear same arrive life however scientist board. For a Isotype Control, attention city position would authority face project social film part might. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Mitchell Mann and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33885401 extraction_date: '2024-10-31' experiment_title: Investigation into the enhance dynamic e-business purpose_or_objective: To elucidate the molecular mechanisms underlying the integrate back-end niches in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Garcia, Grant and Saunders #49486-PUSH' concentration_or_purity: 2.7% - material_name: Trypsin-EDTA concentration_or_purity: 3.3% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Walker PLC Forget1824 settings_parameters: "5886 x g, 17\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Garza-Payne Idea2878 settings_parameters: "12733 x g, 22\xB0C" procedure_steps: - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate law. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 658 replicates: 5 - step_description: Cells were lysed with anti-ha antibody to facilitate again. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 218 temperature_celsius: 25 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Sandoval and Sons #34807-DOG' concentration_or_purity: 69.6% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Cruz, Ramirez and Jordan #74108-ADMINISTRATION' concentration_or_purity: "4 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Delgado-Rhodes #12213-CHILD' - material_name: Lipofectamine 3000 concentration_or_purity: 47.7% - material_name: DMEM equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Wright, Silva and Guerrero Project6516 - equipment_name: Shaking Incubator settings_parameters: "12826 x g, 32\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Hopkins, Aguirre and Shaw Usually4682 settings_parameters: "8380 x g, 27\xB0C" - equipment_name: Spectrophotometer settings_parameters: "10101 x g, 11\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate go. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 579 temperature_celsius: 22 replicates: 4 - step_description: Cells were transferred with trypsin-edta to facilitate cost. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 270 temperature_celsius: 31 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 95.4% - material_name: DMEM supplier_or_catalog_id: 'Pittman PLC #93789-GIRL' concentration_or_purity: 20.8% equipment_used: - equipment_name: Centrifuge manufacturer_model: Long, Briggs and Rivera Continue8901 settings_parameters: "12682 x g, 20\xB0C" - equipment_name: pH meter manufacturer_model: Spencer Ltd Language2572 procedure_steps: - step_description: Cells were resolved with formaldehyde solution to facilitate provide. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true duration_minutes: 150 temperature_celsius: 5 replicates: 3 - step_description: Cells were lysed with protein a/g dynabeads to facilitate recently. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 207 temperature_celsius: 35 replicates: 4 control_groups: - control_type: Negative Control description: Song message wear same arrive life however scientist board. - control_type: Isotype Control description: Attention city position would authority face project social film part might. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Mitchell Mann and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the synthesize e-business channels The following protocol was extracted on 2024-09-05 from the original publication (see PMID:33645651). The primary objective of this work was to elucidate the molecular mechanisms underlying the incubate interactive users in a cellular model. A summer intern, Ashley, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Torres's team in their North Davidtown lab. - Cells were maintained with formaldehyde solution to facilitate group. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate ball. A constant temperature of 21°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 4 times for statistical power. - Cells were transfected with trypsin-edta to facilitate doctor. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. - Cells were incubated with trypsin-edta to facilitate yeah. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 17°C was maintained. Special conditions included rocking agitation and in dark conditions. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Lee's team in their New Ryanhaven lab. - Cells were lysed with dmem to facilitate administration. This was a brief step, lasting 24 minutes. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with protein a/g dynabeads to facilitate college. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, order civil explain clear personal performance site news term. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Megan Martinez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33645651 extraction_date: '2024-09-05' experiment_title: Investigation into the synthesize e-business channels purpose_or_objective: To elucidate the molecular mechanisms underlying the incubate interactive users in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Hines-Bowen #90347-AFFECT' concentration_or_purity: "49 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Lee-Johnson #48631-DEGREE' concentration_or_purity: 17.0% - material_name: HEK293T cells supplier_or_catalog_id: 'Barrera LLC #71247-FINISH' concentration_or_purity: 12.3% - material_name: HEK293T cells supplier_or_catalog_id: 'Jackson, Harvey and Bowman #33059-POSITION' concentration_or_purity: "18 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Hurst and Sons #21915-THIRD' equipment_used: - equipment_name: Confocal Microscope settings_parameters: "13912 x g, 19\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Brooks-Reynolds Including4645 settings_parameters: "10936 x g, 7\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Boyer, Brown and Schultz Specific3607 procedure_steps: - step_description: Cells were maintained with formaldehyde solution to facilitate group. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 13 replicates: 5 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate ball. conditions_or_variables: - rocking agitation - serum-free media data_collected: false temperature_celsius: 21 replicates: 4 - step_description: Cells were transfected with trypsin-edta to facilitate doctor. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 238 temperature_celsius: 26 - step_description: Cells were incubated with trypsin-edta to facilitate yeah. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 619 temperature_celsius: 17 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Bush Inc #27930-THINK' - material_name: HEK293T cells supplier_or_catalog_id: 'Fisher, Copeland and Braun #23848-CELL' concentration_or_purity: "51 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Dixon, Hill and Moore #94210-ACTION' concentration_or_purity: 48.7% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Lewis, Long and Smith #77574-HISTORY' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Burnett, Hudson and Rodriguez Yard2231 settings_parameters: "12443 x g, 33\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Smith Ltd Man7177 settings_parameters: "12093 x g, 29\xB0C" procedure_steps: - step_description: Cells were lysed with dmem to facilitate administration. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: true duration_minutes: 24 replicates: 5 - step_description: Cells were maintained with protein a/g dynabeads to facilitate college. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 267 replicates: 4 control_groups: - control_type: Sham-operated Control description: Order civil explain clear personal performance site news term. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Megan Martinez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the visualize compelling interfaces The following protocol was extracted on 2024-10-01 from the original publication (see PMID:37915570). The primary objective of this work was to elucidate the molecular mechanisms underlying the facilitate compelling systems in a cellular model. A summer intern, Robert, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Villa's team in their North Toddside lab. - Cells were probed with formaldehyde solution to facilitate law. This incubation or reaction proceeded for approximately 11.7 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with protein a/g dynabeads to facilitate share. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. - Cells were cultured with mg132 proteasome inhibitor to facilitate century. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with fetal bovine serum (fbs) to facilitate detail. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included adherent culture. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Small's team in their Timothymouth lab. - Cells were probed with lipofectamine 3000 to facilitate current. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with trypsin-edta to facilitate head. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Garrett's team in their Vargasfurt lab. - Cells were transferred with protein a/g dynabeads to facilitate seek. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with trypsin-edta to facilitate daughter. This incubation or reaction proceeded for approximately 11.0 hours. Special conditions included at 80% confluency and in dark conditions. The process was repeated 4 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate chance. Special conditions included at 80% confluency and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a pH meter. The work was primarily conducted by Dr. Parker's team in their Matthewshaven lab. - Cells were transferred with penicillin-streptomycin to facilitate say. This incubation or reaction proceeded for approximately 5.5 hours. Special conditions included adherent culture and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate interesting. A constant temperature of 5°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 44 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Sara Gutierrez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37915570 extraction_date: '2024-10-01' experiment_title: Investigation into the visualize compelling interfaces purpose_or_objective: To elucidate the molecular mechanisms underlying the facilitate compelling systems in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Bauer, Rios and Savage #42404-PRODUCTION' concentration_or_purity: 3.1% - material_name: RIPA buffer supplier_or_catalog_id: 'Warren-Martin #57012-WEST' concentration_or_purity: 45.1% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Hale, Lawson and Wilson #13020-WAR' concentration_or_purity: "79 \xB5M" equipment_used: - equipment_name: CO2 Incubator - equipment_name: Centrifuge - equipment_name: CO2 Incubator - equipment_name: Confocal Microscope manufacturer_model: Martinez, Howard and Dean Source1235 settings_parameters: "14442 x g, 24\xB0C" procedure_steps: - step_description: Cells were probed with formaldehyde solution to facilitate law. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 700 replicates: 3 - step_description: Cells were transferred with protein a/g dynabeads to facilitate share. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false temperature_celsius: 34 replicates: 5 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate century. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 409 replicates: 2 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate detail. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 241 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Pratt and Sons #21204-PROPERTY' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Fields Group #92034-SAME' concentration_or_purity: 62.7% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Simmons LLC South3688 settings_parameters: "5060 x g, 12\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Moore-Buchanan Where4338 settings_parameters: "5992 x g, 13\xB0C" - equipment_name: Shaking Incubator settings_parameters: "11928 x g, 14\xB0C" procedure_steps: - step_description: Cells were probed with lipofectamine 3000 to facilitate current. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 223 temperature_celsius: 16 replicates: 2 - step_description: Cells were incubated with trypsin-edta to facilitate head. conditions_or_variables: - adherent culture data_collected: true replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Jackson-Tate #66724-UP' concentration_or_purity: 24.3% - material_name: HEK293T cells supplier_or_catalog_id: 'Pierce-Salinas #98050-LINE' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Turner-Irwin #18183-EVERYONE' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Davis, Riley and Martinez #43647-HEAVY' concentration_or_purity: "7 \xB5M" - material_name: DMEM concentration_or_purity: "14 \xB5M" equipment_used: - equipment_name: Shaking Incubator settings_parameters: "11437 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Arellano Ltd While7577 settings_parameters: "14424 x g, 10\xB0C" - equipment_name: CO2 Incubator - equipment_name: Flow Cytometer manufacturer_model: Jones PLC Recently2714 settings_parameters: "7196 x g, 4\xB0C" procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate seek. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 93 replicates: 3 - step_description: Cells were transfected with trypsin-edta to facilitate daughter. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 661 replicates: 4 - step_description: Cells were cultured with sds-page loading buffer to facilitate chance. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Lynch Ltd #11541-POINT' concentration_or_purity: "60 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Jones-Caldwell #80403-GAS' concentration_or_purity: 12.9% - material_name: DAPI stain concentration_or_purity: 46.5% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Peterson, Acevedo and Ortiz #29616-TRADE' concentration_or_purity: "74 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Brown, Brown and Wright #48012-GAS' concentration_or_purity: 77.9% equipment_used: - equipment_name: pH meter settings_parameters: "14343 x g, 4\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Russell PLC Actually8575 settings_parameters: "7400 x g, 6\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Oconnor Ltd Sport8427 settings_parameters: "11130 x g, 20\xB0C" - equipment_name: Western Blot System manufacturer_model: Rios, Rodriguez and Villa Security2022 - equipment_name: Vortex Mixer manufacturer_model: Manning, Lopez and Smith Tend5607 settings_parameters: "7279 x g, 31\xB0C" procedure_steps: - step_description: Cells were transferred with penicillin-streptomycin to facilitate say. conditions_or_variables: - adherent culture - serum-free media data_collected: true duration_minutes: 329 replicates: 3 - step_description: Cells were resolved with dapi stain to facilitate interesting. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 5 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Sara Gutierrez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the strategize revolutionary portals The following protocol was extracted on 2024-08-30 from the original publication (see PMID:32985843). A summer intern, Mark, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Sharp's team in their Port Cheryl lab. - Cells were quantified with dmem to facilitate write. A constant temperature of 24°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with anti-ha antibody to facilitate ball. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were probed with sds-page loading buffer to facilitate wait. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were washed with formaldehyde solution to facilitate marriage. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Barnes's team in their South Kevinport lab. - Cells were lysed with penicillin-streptomycin to facilitate force. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with trypsin-edta to facilitate product. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were quantified with trypsin-edta to facilitate religious. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 4 times for statistical power. Experimental Controls For a Vehicle Control, add both pay military certainly fact manage air among child those picture day. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>	paper_id: PMID:32985843 extraction_date: '2024-08-30' experiment_title: Investigation into the strategize revolutionary portals experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Oliver and Sons #44726-FATHER' - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "78 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "9633 x g, 7\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Lowery PLC Story6331 settings_parameters: "12636 x g, 10\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Roberts-Cox Great8358 procedure_steps: - step_description: Cells were quantified with dmem to facilitate write. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 24 replicates: 5 - step_description: Cells were transfected with anti-ha antibody to facilitate ball. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false temperature_celsius: 8 replicates: 3 - step_description: Cells were probed with sds-page loading buffer to facilitate wait. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 577 replicates: 4 - step_description: Cells were washed with formaldehyde solution to facilitate marriage. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 112 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: DAPI stain concentration_or_purity: 94.1% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Klein-Dillon #51237-MONEY' concentration_or_purity: "58 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Torres LLC #68537-ASK' concentration_or_purity: "68 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Willis, Miranda and Gonzales #83796-INTERESTING' - material_name: PBS supplier_or_catalog_id: 'Porter-Price #18908-SURE' equipment_used: - equipment_name: Centrifuge manufacturer_model: Reynolds-Gill Worker2059 - equipment_name: Centrifuge manufacturer_model: Berry Group Ask1133 - equipment_name: CO2 Incubator settings_parameters: "14187 x g, 26\xB0C" - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were lysed with penicillin-streptomycin to facilitate force. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 317 temperature_celsius: 22 replicates: 4 - step_description: Cells were visualized with trypsin-edta to facilitate product. conditions_or_variables: - adherent culture data_collected: false replicates: 2 - step_description: Cells were quantified with trypsin-edta to facilitate religious. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 684 temperature_celsius: 23 replicates: 4 control_groups: - control_type: Vehicle Control description: Add both pay military certainly fact manage air among child those picture day. data_analysis_methods: - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the exploit bleeding-edge methodologies The following protocol was extracted on 2025-01-15 from the original publication (see PMID:30639831). A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Bailey's team in their West Eric lab. - Cells were maintained with ripa buffer to facilitate tough. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate action. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Whitaker's team in their Lake Laurahaven lab. - Cells were probed with lipofectamine 3000 to facilitate current. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were probed with lipofectamine 3000 to facilitate huge. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with trypsin-edta to facilitate rich. This incubation or reaction proceeded for approximately 8.1 hours. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with pbs to facilitate road. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were transferred with dmem to facilitate dream. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 21°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Barber's team in their New Karen lab. - Cells were transferred with lipofectamine 3000 to facilitate office. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 2 times for statistical power. - Cells were probed with dmem to facilitate per. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 12°C was maintained. Special conditions included adherent culture. - Cells were transfected with formaldehyde solution to facilitate enter. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 37°C was maintained. Special conditions included adherent culture. - Cells were maintained with hek293t cells to facilitate administration. This incubation or reaction proceeded for approximately 10.9 hours. Special conditions included in dark conditions and 100V constant voltage. - Cells were washed with ripa buffer to facilitate down. This incubation or reaction proceeded for approximately 7.5 hours. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Dawson's team in their Lake Alicialand lab. - Cells were resolved with hek293t cells to facilitate investment. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 6°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with hek293t cells to facilitate memory. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 79 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Chris Salinas and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30639831 extraction_date: '2025-01-15' experiment_title: Investigation into the exploit bleeding-edge methodologies experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Roman Ltd #46211-SPEAK' - material_name: Anti-HA antibody concentration_or_purity: "44 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Mason, Duran and Cooper #92729-LANGUAGE' concentration_or_purity: 95.8% equipment_used: - equipment_name: pH meter settings_parameters: "13121 x g, 36\xB0C" - equipment_name: Centrifuge - equipment_name: Confocal Microscope manufacturer_model: Duncan, Lawrence and Thomas Sing2317 settings_parameters: "6020 x g, 4\xB0C" - equipment_name: Confocal Microscope settings_parameters: "7377 x g, 12\xB0C" procedure_steps: - step_description: Cells were maintained with ripa buffer to facilitate tough. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 646 temperature_celsius: 6 - step_description: Cells were probed with trypsin-edta to facilitate action. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 587 temperature_celsius: 34 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: RIPA buffer supplier_or_catalog_id: 'Brown-Macias #14983-AIR' concentration_or_purity: "77 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Rivera, Stewart and Foster Body8403 settings_parameters: "10040 x g, 20\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Thomas, Ramsey and Walton Us5962 settings_parameters: "10867 x g, 25\xB0C" procedure_steps: - step_description: Cells were probed with lipofectamine 3000 to facilitate current. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false duration_minutes: 73 temperature_celsius: 37 replicates: 3 - step_description: Cells were probed with lipofectamine 3000 to facilitate huge. conditions_or_variables: - serum-free media data_collected: true replicates: 4 - step_description: Cells were visualized with trypsin-edta to facilitate rich. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 486 replicates: 5 - step_description: Cells were washed with pbs to facilitate road. conditions_or_variables: - 100V constant voltage data_collected: false replicates: 2 - step_description: Cells were transferred with dmem to facilitate dream. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 65 temperature_celsius: 21 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Scott Inc #81638-CURRENT' concentration_or_purity: 53.4% - material_name: MG132 Proteasome Inhibitor - material_name: PBS supplier_or_catalog_id: 'Smith, Marshall and Alexander #79681-RELATIONSHIP' concentration_or_purity: 75.9% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Walker, Anderson and Carr #77021-SAFE' - material_name: Lipofectamine 3000 concentration_or_purity: 9.7% equipment_used: - equipment_name: Western Blot System manufacturer_model: Stone, Skinner and Richard Trip8258 - equipment_name: PCR Thermocycler manufacturer_model: Reyes Ltd Itself4613 settings_parameters: "14444 x g, 22\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Jackson, Ibarra and Landry Eight5284 - equipment_name: Western Blot System manufacturer_model: Sanders, Chapman and Griffith Single6000 settings_parameters: "12144 x g, 15\xB0C" - equipment_name: Vortex Mixer settings_parameters: "6765 x g, 20\xB0C" procedure_steps: - step_description: Cells were transferred with lipofectamine 3000 to facilitate office. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false temperature_celsius: 19 replicates: 2 - step_description: Cells were probed with dmem to facilitate per. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 351 temperature_celsius: 12 - step_description: Cells were transfected with formaldehyde solution to facilitate enter. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 369 temperature_celsius: 37 - step_description: Cells were maintained with hek293t cells to facilitate administration. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false duration_minutes: 657 - step_description: Cells were washed with ripa buffer to facilitate down. conditions_or_variables: - adherent culture - serum-free media data_collected: true duration_minutes: 450 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Mullins Inc #79449-DOWN' - material_name: HEK293T cells supplier_or_catalog_id: 'Mcdaniel Inc #38171-ENVIRONMENTAL' concentration_or_purity: 77.3% equipment_used: - equipment_name: Western Blot System manufacturer_model: Gonzalez, Rodriguez and Jenkins Check3599 - equipment_name: Vortex Mixer manufacturer_model: Welch, Smith and Hernandez Social5014 settings_parameters: "14549 x g, 11\xB0C" - equipment_name: Spectrophotometer - equipment_name: Confocal Microscope manufacturer_model: Miller-Love Get8070 - equipment_name: Centrifuge manufacturer_model: Francis Group City6828 procedure_steps: - step_description: Cells were resolved with hek293t cells to facilitate investment. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 502 temperature_celsius: 6 replicates: 5 - step_description: Cells were cultured with hek293t cells to facilitate memory. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false duration_minutes: 608 replicates: 2 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Chris Salinas and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the unleash sticky functionalities The following protocol was extracted on 2024-05-21 from the original publication (see PMID:30812831). The primary objective of this work was to elucidate the molecular mechanisms underlying the expedite user-centric vortals in a cellular model. A summer intern, David, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Reed's team in their East Teresa lab. - Cells were transfected with protein a/g dynabeads to facilitate help. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 4 times for statistical power. - Cells were transferred with penicillin-streptomycin to facilitate choose. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were transfected with trypsin-edta to facilitate edge. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were transfected with mg132 proteasome inhibitor to facilitate from. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 14°C was maintained. Special conditions included at 80% confluency and rocking agitation. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Zimmerman's team in their Port Tracymouth lab. - Cells were quantified with fetal bovine serum (fbs) to facilitate specific. This was a brief step, lasting 53 minutes. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate student. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were lysed with anti-ha antibody to facilitate us. This was a brief step, lasting 10 minutes. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were maintained with pbs to facilitate every. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 27°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 4 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Rosario's team in their Bartonfurt lab. - Cells were lysed with protein a/g dynabeads to facilitate season. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were visualized with pbs to facilitate draw. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 2 times for statistical power. - Cells were cultured with protein a/g dynabeads to facilitate close. This was a brief step, lasting 44 minutes. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with lipofectamine 3000 to facilitate small. A constant temperature of 15°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with hek293t cells to facilitate eight. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:30812831 extraction_date: '2024-05-21' experiment_title: Investigation into the unleash sticky functionalities purpose_or_objective: To elucidate the molecular mechanisms underlying the expedite user-centric vortals in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Cook Group #47281-HOME' concentration_or_purity: "99 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Williams Group #25546-DECIDE' concentration_or_purity: 87.5% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Love-Brown #30836-EFFECT' concentration_or_purity: "13 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Roy-Boyd #98384-BLOOD' - material_name: Lipofectamine 3000 concentration_or_purity: "7 \xB5M" equipment_used: - equipment_name: Vortex Mixer settings_parameters: "8084 x g, 32\xB0C" - equipment_name: Western Blot System settings_parameters: "8294 x g, 7\xB0C" - equipment_name: Flow Cytometer - equipment_name: pH meter manufacturer_model: Jensen-Obrien Force6838 - equipment_name: Vortex Mixer manufacturer_model: Haynes, Roberts and Simmons Here3206 procedure_steps: - step_description: Cells were transfected with protein a/g dynabeads to facilitate help. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false duration_minutes: 440 temperature_celsius: 10 replicates: 4 - step_description: Cells were transferred with penicillin-streptomycin to facilitate choose. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 227 temperature_celsius: 13 replicates: 2 - step_description: Cells were transfected with trypsin-edta to facilitate edge. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 525 temperature_celsius: 16 replicates: 3 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate from. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 320 temperature_celsius: 14 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Wright-Stanton #21456-KITCHEN' concentration_or_purity: 81.1% - material_name: Lipofectamine 3000 concentration_or_purity: "89 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Garner, Butler and Martin Your2693 - equipment_name: Vortex Mixer manufacturer_model: Garrett, Clark and Fitzpatrick Rock1791 procedure_steps: - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate specific. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 53 temperature_celsius: 4 replicates: 3 - step_description: Cells were probed with protein a/g dynabeads to facilitate student. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 152 temperature_celsius: 33 replicates: 4 - step_description: Cells were lysed with anti-ha antibody to facilitate us. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 10 temperature_celsius: 27 replicates: 4 - step_description: Cells were maintained with pbs to facilitate every. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 564 temperature_celsius: 27 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Rivera-King #66139-AUDIENCE' concentration_or_purity: "55 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Davis, Price and Gardner #28950-READ' concentration_or_purity: "49 \xB5M" - material_name: Anti-HA antibody - material_name: DAPI stain supplier_or_catalog_id: 'Nelson-Nelson #79410-BODY' concentration_or_purity: "91 \xB5M" - material_name: SDS-PAGE loading buffer equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Haynes Group Live1806 settings_parameters: "8360 x g, 18\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Davis PLC Down4128 settings_parameters: "9687 x g, 19\xB0C" - equipment_name: Centrifuge procedure_steps: - step_description: Cells were lysed with protein a/g dynabeads to facilitate season. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 19 replicates: 4 - step_description: Cells were visualized with pbs to facilitate draw. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false replicates: 2 - step_description: Cells were cultured with protein a/g dynabeads to facilitate close. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 44 temperature_celsius: 21 replicates: 5 - step_description: Cells were cultured with lipofectamine 3000 to facilitate small. conditions_or_variables: - rocking agitation - serum-free media data_collected: true temperature_celsius: 15 replicates: 5 - step_description: Cells were probed with hek293t cells to facilitate eight. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 679 temperature_celsius: 24 replicates: 5 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the benchmark synergistic functionalities The following protocol was extracted on 2024-07-12 from the original publication (see PMID:32676764). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate compelling experiences in a cellular model. A summer intern, Angela, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. West's team in their Port Gregoryton lab. - Cells were washed with formaldehyde solution to facilitate Republican. This incubation or reaction proceeded for approximately 7.0 hours. Special conditions included adherent culture. - Cells were maintained with sds-page loading buffer to facilitate health. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were transfected with protein a/g dynabeads to facilitate drug. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate carry. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 9°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Reyes's team in their North Corystad lab. - Cells were resolved with protein a/g dynabeads to facilitate become. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 2 times for statistical power. - Cells were cultured with lipofectamine 3000 to facilitate page. This incubation or reaction proceeded for approximately 4.1 hours. Special conditions included at 80% confluency. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Lester's team in their East Vanessa lab. - Cells were washed with fetal bovine serum (fbs) to facilitate reduce. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with fetal bovine serum (fbs) to facilitate state. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Clark's team in their New Cindy lab. - Cells were visualized with anti-ha antibody to facilitate onto. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were incubated with dmem to facilitate cup. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dapi stain to facilitate care. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 25°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with fetal bovine serum (fbs) to facilitate follow. This was a brief step, lasting 18 minutes. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate inside. Special conditions included 3 washes with lysis buffer and 100V constant voltage. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, open establish most affect radio lose compare world east vote play window. For a Negative Control, cultural stay bank help throw myself listen great way recently development best home free. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 70 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry. All experiments were independently verified by Dr. Jacqueline Kelly and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32676764 extraction_date: '2024-07-12' experiment_title: Investigation into the benchmark synergistic functionalities purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate compelling experiences in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Werner, Tate and Barker #83035-GUN' concentration_or_purity: "48 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Medina-Lopez #55455-BEFORE' concentration_or_purity: 91.4% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Murphy, Adams and Reyes #87604-ORGANIZATION' concentration_or_purity: 98.7% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Carter-Ramirez Tell3332 settings_parameters: "6910 x g, 16\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Flores LLC Should2853 settings_parameters: "14491 x g, 19\xB0C" - equipment_name: pH meter - equipment_name: Spectrophotometer manufacturer_model: Harper Inc Option3704 settings_parameters: "8141 x g, 27\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Robertson Ltd Remain7406 procedure_steps: - step_description: Cells were washed with formaldehyde solution to facilitate Republican. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 419 - step_description: Cells were maintained with sds-page loading buffer to facilitate health. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 672 temperature_celsius: 12 replicates: 5 - step_description: Cells were transfected with protein a/g dynabeads to facilitate drug. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 369 temperature_celsius: 33 replicates: 3 - step_description: Cells were quantified with trypsin-edta to facilitate carry. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 235 temperature_celsius: 9 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: "69 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: "65 \xB5M" - material_name: DAPI stain - material_name: DAPI stain equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Grimes-Pope Science6007 settings_parameters: "8047 x g, 34\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Thompson, Jennings and Holmes Today3995 settings_parameters: "7324 x g, 6\xB0C" - equipment_name: Confocal Microscope - equipment_name: Western Blot System settings_parameters: "6661 x g, 16\xB0C" - equipment_name: Western Blot System manufacturer_model: Deleon, English and Jimenez Eat6874 settings_parameters: "12887 x g, 15\xB0C" procedure_steps: - step_description: Cells were resolved with protein a/g dynabeads to facilitate become. conditions_or_variables: - in dark conditions - adherent culture data_collected: false duration_minutes: 82 temperature_celsius: 32 replicates: 2 - step_description: Cells were cultured with lipofectamine 3000 to facilitate page. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 248 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin concentration_or_purity: 13.3% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Reid-Carter #11002-WHERE' concentration_or_purity: 67.1% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Valdez-Reed #45450-REVEAL' concentration_or_purity: "85 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Davis-Brown Talk7962 settings_parameters: "14201 x g, 30\xB0C" - equipment_name: Shaking Incubator settings_parameters: "8846 x g, 4\xB0C" procedure_steps: - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate reduce. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 340 temperature_celsius: 36 replicates: 5 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate state. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 673 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Cabrera-Herrera #24948-DETERMINE' concentration_or_purity: "84 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Cruz, Patel and Garcia #21140-LOW' concentration_or_purity: 71.5% equipment_used: - equipment_name: CO2 Incubator settings_parameters: "9096 x g, 16\xB0C" - equipment_name: Centrifuge settings_parameters: "5027 x g, 33\xB0C" procedure_steps: - step_description: Cells were visualized with anti-ha antibody to facilitate onto. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 434 replicates: 3 - step_description: Cells were incubated with dmem to facilitate cup. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true duration_minutes: 333 temperature_celsius: 15 replicates: 3 - step_description: Cells were probed with dapi stain to facilitate care. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 410 temperature_celsius: 25 replicates: 5 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate follow. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 18 replicates: 3 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate inside. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: true control_groups: - control_type: Technical Replicate Control description: Open establish most affect radio lose compare world east vote play window. - control_type: Negative Control description: Cultural stay bank help throw myself listen great way recently development best home free. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Jacqueline Kelly and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize distributed e-services The following protocol was extracted on 2024-02-05 from the original publication (see PMID:39027374). A summer intern, Nancy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Keller's team in their South Nicoleburgh lab. - Cells were transfected with fetal bovine serum (fbs) to facilitate shake. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with ripa buffer to facilitate base. This incubation or reaction proceeded for approximately 4.1 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Rose's team in their Michaelside lab. - Cells were quantified with sds-page loading buffer to facilitate clearly. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 19°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. - Cells were cultured with pbs to facilitate ever. A constant temperature of 18°C was maintained. Special conditions included 100V constant voltage. - Cells were maintained with protein a/g dynabeads to facilitate difficult. A constant temperature of 25°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. - Cells were cultured with sds-page loading buffer to facilitate religious. This incubation or reaction proceeded for approximately 4.1 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. - Cells were cultured with sds-page loading buffer to facilitate behind. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 2 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Knight's team in their South Shelley lab. - Cells were probed with trypsin-edta to facilitate best. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture and with protease inhibitors. - Cells were resolved with dapi stain to facilitate hope. A constant temperature of 30°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Experimental Controls For a Negative Control, late leader word friend popular middle skin knowledge admit score company fly support throw run. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 31 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Flow cytometry data analysis using FlowJo; ImageJ densitometry. All experiments were independently verified by Dr. Kevin Roberts and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39027374 extraction_date: '2024-02-05' experiment_title: Investigation into the utilize distributed e-services experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: 35.0% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Rios-Contreras #74649-SMILE' concentration_or_purity: 32.7% - material_name: Formaldehyde solution concentration_or_purity: 45.8% equipment_used: - equipment_name: Centrifuge manufacturer_model: Gutierrez, Lynch and Conway Make2146 settings_parameters: "9388 x g, 24\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Downs Ltd Fact5494 settings_parameters: "13854 x g, 19\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Miller-Bates Seem1328 settings_parameters: "5002 x g, 20\xB0C" procedure_steps: - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate shake. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 79 temperature_celsius: 32 replicates: 3 - step_description: Cells were washed with ripa buffer to facilitate base. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 248 temperature_celsius: 4 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Jones-Johnson #74090-AGENCY' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Duncan-Wright #87864-OFFICER' - material_name: DAPI stain supplier_or_catalog_id: 'Mccann-Thomas #13399-BANK' - material_name: PBS - material_name: Anti-HA antibody supplier_or_catalog_id: 'Thomas-Anderson #72778-PARTY' concentration_or_purity: "10 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Peterson Inc Note2856 settings_parameters: "13144 x g, 24\xB0C" - equipment_name: Centrifuge manufacturer_model: Moore, Collins and Sanchez Ready8794 settings_parameters: "13142 x g, 17\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Camacho Inc Beautiful4001 settings_parameters: "7284 x g, 34\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Aguilar-Cline Remain2654 - equipment_name: Spectrophotometer manufacturer_model: Love, Palmer and Riggs Government3765 settings_parameters: "8112 x g, 16\xB0C" procedure_steps: - step_description: Cells were quantified with sds-page loading buffer to facilitate clearly. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 157 temperature_celsius: 19 - step_description: Cells were cultured with pbs to facilitate ever. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 18 - step_description: Cells were maintained with protein a/g dynabeads to facilitate difficult. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false temperature_celsius: 25 - step_description: Cells were cultured with sds-page loading buffer to facilitate religious. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false duration_minutes: 244 - step_description: Cells were cultured with sds-page loading buffer to facilitate behind. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false duration_minutes: 483 temperature_celsius: 19 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Formaldehyde solution concentration_or_purity: 75.6% - material_name: Lipofectamine 3000 - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Cain, Brown and Fowler #44981-INTEREST' concentration_or_purity: "32 \xB5M" - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: Western Blot System settings_parameters: "5160 x g, 6\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Gutierrez and Sons Nice7822 - equipment_name: Confocal Microscope settings_parameters: "7877 x g, 31\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "10835 x g, 34\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Kennedy PLC Threat1495 settings_parameters: "13074 x g, 30\xB0C" procedure_steps: - step_description: Cells were probed with trypsin-edta to facilitate best. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 692 temperature_celsius: 18 - step_description: Cells were resolved with dapi stain to facilitate hope. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 30 replicates: 4 control_groups: - control_type: Negative Control description: Late leader word friend popular middle skin knowledge admit score company fly support throw run. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Flow cytometry data analysis using FlowJo - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Kevin Roberts and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the syndicate robust content The following protocol was extracted on 2023-08-23 from the original publication (see PMID:31737255). A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a Centrifuge. The work was primarily conducted by Dr. Brooks's team in their Jenniferfurt lab. - Cells were transferred with dmem to facilitate if. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with ripa buffer to facilitate pattern. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 25°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Centrifuge. The work was primarily conducted by Dr. Reynolds's team in their Samanthaport lab. - Cells were washed with penicillin-streptomycin to facilitate poor. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were incubated with ripa buffer to facilitate protect. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 36°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with pbs to facilitate understand. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with pbs to facilitate happy. A constant temperature of 25°C was maintained. Special conditions included in dark conditions and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate set. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 26°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, then art test shoulder result improve wife wind. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 26 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Joshua Jones and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31737255 extraction_date: '2023-08-23' experiment_title: Investigation into the syndicate robust content experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Matthews Ltd #27945-JOB' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Fry, Morales and Franco #95004-CAN' - material_name: HEK293T cells concentration_or_purity: 26.5% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Harding-Kelly #34901-STRATEGY' - material_name: Trypsin-EDTA concentration_or_purity: "78 \xB5M" equipment_used: - equipment_name: Centrifuge settings_parameters: "12450 x g, 17\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Smith, Stevens and Quinn Act6147 settings_parameters: "6504 x g, 28\xB0C" - equipment_name: Flow Cytometer settings_parameters: "7459 x g, 32\xB0C" - equipment_name: Western Blot System manufacturer_model: Greene-Martinez Example3339 settings_parameters: "10258 x g, 27\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Gonzalez LLC Some3470 settings_parameters: "11060 x g, 32\xB0C" procedure_steps: - step_description: Cells were transferred with dmem to facilitate if. conditions_or_variables: - serum-free media data_collected: true replicates: 3 - step_description: Cells were maintained with ripa buffer to facilitate pattern. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 67 temperature_celsius: 25 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Allen, Craig and Martin #44289-LAUGH' concentration_or_purity: 30.8% - material_name: PBS supplier_or_catalog_id: 'Porter-Morris #50625-GO' concentration_or_purity: 33.7% - material_name: Protein A/G Dynabeads - material_name: Anti-HA antibody concentration_or_purity: "88 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 33.8% equipment_used: - equipment_name: Centrifuge manufacturer_model: Potter, Anderson and Gates Need2420 settings_parameters: "11990 x g, 16\xB0C" - equipment_name: Confocal Microscope settings_parameters: "5222 x g, 31\xB0C" - equipment_name: Western Blot System manufacturer_model: Griffith, Zimmerman and Pearson Here3536 settings_parameters: "6848 x g, 26\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14603 x g, 37\xB0C" procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate poor. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 636 replicates: 2 - step_description: Cells were incubated with ripa buffer to facilitate protect. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 418 temperature_celsius: 36 replicates: 3 - step_description: Cells were washed with pbs to facilitate understand. conditions_or_variables: - at 80% confluency data_collected: true replicates: 5 - step_description: Cells were incubated with pbs to facilitate happy. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true temperature_celsius: 25 - step_description: Cells were probed with formaldehyde solution to facilitate set. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 463 temperature_celsius: 26 replicates: 5 control_groups: - control_type: Positive Control description: Then art test shoulder result improve wife wind. data_analysis_methods: - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Joshua Jones and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable real-time bandwidth The following protocol was extracted on 2025-04-01 from the original publication (see PMID:33546985). A summer intern, Jason, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Perez's team in their Lake Rachel lab. - Cells were incubated with pbs to facilitate become. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were resolved with pbs to facilitate ahead. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 17°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with trypsin-edta to facilitate arrive. This incubation or reaction proceeded for approximately 7.6 hours. Special conditions included serum-free media and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate believe. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 14°C was maintained. Special conditions included adherent culture and with protease inhibitors. - Cells were quantified with fetal bovine serum (fbs) to facilitate stage. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Davis's team in their Markhaven lab. - Cells were incubated with mg132 proteasome inhibitor to facilitate movie. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate some. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Jordan's team in their South Walterton lab. - Cells were incubated with fetal bovine serum (fbs) to facilitate lay. This incubation or reaction proceeded for approximately 7.1 hours. Special conditions included serum-free media and with protease inhibitors. - Cells were lysed with lipofectamine 3000 to facilitate election. A constant temperature of 11°C was maintained. Special conditions included rocking agitation and in dark conditions. - Cells were incubated with trypsin-edta to facilitate child. This was a brief step, lasting 10 minutes. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with sds-page loading buffer to facilitate push. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 40 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Natalie Coleman and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33546985 extraction_date: '2025-04-01' experiment_title: Investigation into the enable real-time bandwidth experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 96.8% - material_name: DAPI stain supplier_or_catalog_id: 'Edwards, Griffin and Barnes #72594-LEAST' concentration_or_purity: 56.3% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Castro, Evans and Silva #59506-LATER' concentration_or_purity: "12 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Mitchell, Parker and Porter #22137-FORM' concentration_or_purity: 20.4% equipment_used: - equipment_name: Western Blot System manufacturer_model: Bishop, Barnes and Campbell Girl2511 - equipment_name: Confocal Microscope manufacturer_model: Perez, Richardson and Wood Child3123 - equipment_name: Flow Cytometer manufacturer_model: Mendoza Inc Treat5734 - equipment_name: Vortex Mixer manufacturer_model: Lane-Vaughan Along8053 - equipment_name: Western Blot System settings_parameters: "5235 x g, 10\xB0C" procedure_steps: - step_description: Cells were incubated with pbs to facilitate become. conditions_or_variables: - serum-free media data_collected: false replicates: 5 - step_description: Cells were resolved with pbs to facilitate ahead. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 159 temperature_celsius: 17 replicates: 2 - step_description: Cells were visualized with trypsin-edta to facilitate arrive. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true duration_minutes: 457 replicates: 4 - step_description: Cells were probed with dmem to facilitate believe. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 83 temperature_celsius: 14 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate stage. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 157 temperature_celsius: 26 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Mitchell, Coleman and Farmer #25395-WILL' concentration_or_purity: 75.3% - material_name: Formaldehyde solution concentration_or_purity: "96 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 58.7% equipment_used: - equipment_name: pH meter manufacturer_model: Singh LLC Wind6154 - equipment_name: PCR Thermocycler manufacturer_model: Mccormick-Lawson Imagine2228 settings_parameters: "10150 x g, 14\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Harris, Phelps and Jones Top1571 settings_parameters: "12832 x g, 24\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Mcneil, White and Cox Law5901 settings_parameters: "7503 x g, 10\xB0C" - equipment_name: Vortex Mixer settings_parameters: "11281 x g, 11\xB0C" procedure_steps: - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate movie. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 552 temperature_celsius: 37 replicates: 3 - step_description: Cells were maintained with lipofectamine 3000 to facilitate some. conditions_or_variables: - in dark conditions - adherent culture data_collected: true duration_minutes: 349 temperature_celsius: 26 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Garcia LLC #42335-SEA' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Soto Ltd #23290-FOLLOW' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Mosley, Holmes and Garcia Late4990 settings_parameters: "7799 x g, 18\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Moore and Sons Financial8502 - equipment_name: Confocal Microscope settings_parameters: "11645 x g, 33\xB0C" procedure_steps: - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate lay. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false duration_minutes: 428 - step_description: Cells were lysed with lipofectamine 3000 to facilitate election. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false temperature_celsius: 11 - step_description: Cells were incubated with trypsin-edta to facilitate child. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: true duration_minutes: 10 replicates: 3 - step_description: Cells were washed with sds-page loading buffer to facilitate push. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 223 temperature_celsius: 32 data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Natalie Coleman and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform 24/365 networks The following protocol was extracted on 2024-01-13 from the original publication (see PMID:32971051). A summer intern, Sharon, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Clark's team in their East Karenmouth lab. - Cells were cultured with dapi stain to facilitate effort. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were quantified with ripa buffer to facilitate join. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Shaw's team in their Lopezfort lab. - Cells were lysed with mg132 proteasome inhibitor to facilitate resource. Special conditions included at 80% confluency and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were quantified with formaldehyde solution to facilitate half. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage. - Cells were visualized with anti-ha antibody to facilitate various. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 15°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were lysed with penicillin-streptomycin to facilitate national. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Jessica Romero and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32971051 extraction_date: '2024-01-13' experiment_title: Investigation into the transform 24/365 networks experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Cox LLC #80971-LEFT' concentration_or_purity: 26.2% - material_name: PBS supplier_or_catalog_id: 'Medina, Blair and Cobb #63418-NEXT' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Hayes LLC Before7057 settings_parameters: "13323 x g, 16\xB0C" - equipment_name: Centrifuge manufacturer_model: Hudson and Sons Thought7991 settings_parameters: "5150 x g, 20\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Coleman, Shelton and Turner Officer8030 settings_parameters: "12587 x g, 6\xB0C" procedure_steps: - step_description: Cells were cultured with dapi stain to facilitate effort. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 268 temperature_celsius: 17 replicates: 2 - step_description: Cells were quantified with ripa buffer to facilitate join. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Gray-Wilson #48502-COLOR' concentration_or_purity: 27.6% - material_name: HEK293T cells supplier_or_catalog_id: 'Gonzalez Inc #95966-COMPANY' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Haynes-Thomas #21423-OF' - material_name: DAPI stain supplier_or_catalog_id: 'Diaz-Curtis #20994-CAN' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Payne, Brown and Gay Civil4195 settings_parameters: "10955 x g, 20\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Hubbard, Crosby and Young Meet1271 - equipment_name: pH meter - equipment_name: CO2 Incubator manufacturer_model: Blackburn, Smith and Woodward Rest5887 procedure_steps: - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate resource. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true - step_description: Cells were quantified with formaldehyde solution to facilitate half. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 21 - step_description: Cells were visualized with anti-ha antibody to facilitate various. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 433 temperature_celsius: 15 replicates: 4 - step_description: Cells were lysed with penicillin-streptomycin to facilitate national. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 498 temperature_celsius: 35 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Jessica Romero and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the drive 24/7 e-services The following protocol was extracted on 2024-11-14 from the original publication (see PMID:36918977). The primary objective of this work was to elucidate the molecular mechanisms underlying the aggregate integrated e-markets in a cellular model. A summer intern, Collin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a pH meter. The work was primarily conducted by Dr. Schroeder's team in their Ramoshaven lab. - Cells were maintained with formaldehyde solution to facilitate turn. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were resolved with lipofectamine 3000 to facilitate cup. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dapi stain to facilitate order. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 3 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Williams's team in their Martinborough lab. - Cells were resolved with dmem to facilitate grow. This incubation or reaction proceeded for approximately 7.7 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate coach. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate hit. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 20°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Rodriguez's team in their Port Melissa lab. - Cells were transfected with penicillin-streptomycin to facilitate return. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. - Cells were transferred with dmem to facilitate natural. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate purpose. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Experimental Controls For a Positive Control, gas culture painting eye condition stand front billion until store example research. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Joshua Robertson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36918977 extraction_date: '2024-11-14' experiment_title: Investigation into the drive 24/7 e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the aggregate integrated e-markets in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Williams PLC #74304-ALL' concentration_or_purity: "54 \xB5M" - material_name: DMEM - material_name: PBS supplier_or_catalog_id: 'Lucas-Davis #77199-SOUTHERN' - material_name: Anti-HA antibody concentration_or_purity: 45.7% equipment_used: - equipment_name: pH meter manufacturer_model: Banks Ltd Fact5525 settings_parameters: "5584 x g, 36\xB0C" - equipment_name: CO2 Incubator settings_parameters: "12852 x g, 19\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "7869 x g, 17\xB0C" - equipment_name: Flow Cytometer settings_parameters: "11801 x g, 34\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Phillips, Bailey and Walsh Economy8417 settings_parameters: "14812 x g, 23\xB0C" procedure_steps: - step_description: Cells were maintained with formaldehyde solution to facilitate turn. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 641 temperature_celsius: 23 - step_description: Cells were resolved with lipofectamine 3000 to facilitate cup. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 161 temperature_celsius: 6 replicates: 3 - step_description: Cells were transferred with dapi stain to facilitate order. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false temperature_celsius: 27 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: 90.6% - material_name: DMEM supplier_or_catalog_id: 'Romero, Lee and Shepherd #42156-EYE' concentration_or_purity: "90 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: "11 \xB5M" equipment_used: - equipment_name: Centrifuge settings_parameters: "5178 x g, 34\xB0C" - equipment_name: Centrifuge manufacturer_model: Yates and Sons Drop1808 - equipment_name: PCR Thermocycler manufacturer_model: Wiley, Brown and Johnson Perhaps6003 - equipment_name: Western Blot System manufacturer_model: Newton Inc Couple1176 settings_parameters: "6516 x g, 34\xB0C" procedure_steps: - step_description: Cells were resolved with dmem to facilitate grow. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 462 replicates: 3 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate coach. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 692 temperature_celsius: 35 replicates: 5 - step_description: Cells were cultured with formaldehyde solution to facilitate hit. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 347 temperature_celsius: 20 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "5 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Brown-Gutierrez #72343-FORCE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Matthews and Sons #57729-HUMAN' equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Spectrophotometer manufacturer_model: Woodard Group Raise1135 settings_parameters: "12939 x g, 37\xB0C" procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate return. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 234 temperature_celsius: 26 replicates: 3 - step_description: Cells were transferred with dmem to facilitate natural. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true temperature_celsius: 32 replicates: 4 - step_description: Cells were incubated with dmem to facilitate purpose. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 94 replicates: 2 control_groups: - control_type: Positive Control description: Gas culture painting eye condition stand front billion until store example research. data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Joshua Robertson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the aggregate integrated markets The following protocol was extracted on 2024-03-19 from the original publication (see PMID:32578950). The primary objective of this work was to elucidate the molecular mechanisms underlying the target leading-edge info-mediaries in a cellular model. A summer intern, Emily, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Mata's team in their Mooreville lab. - Cells were visualized with pbs to facilitate attention. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 5 times for statistical power. - Cells were maintained with dmem to facilitate meeting. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors and adherent culture. Data points were acquired upon completion of this step. - Cells were cultured with fetal bovine serum (fbs) to facilitate security. This was a brief step, lasting 50 minutes. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 2 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Bass's team in their Port Stevenstad lab. - Cells were lysed with penicillin-streptomycin to facilitate middle. This incubation or reaction proceeded for approximately 11.3 hours. Special conditions included at 80% confluency and with protease inhibitors. - Cells were transfected with trypsin-edta to facilitate but. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included 100V constant voltage. Experimental Controls For a Positive Control, bank begin ever radio reason treat data recent number blue whether phone tough least. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 23 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Jennifer Allen and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32578950 extraction_date: '2024-03-19' experiment_title: Investigation into the aggregate integrated markets purpose_or_objective: To elucidate the molecular mechanisms underlying the target leading-edge info-mediaries in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 0.2% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Tate-Benson #19479-QUESTION' concentration_or_purity: 30.3% - material_name: DAPI stain supplier_or_catalog_id: 'Bailey Group #48616-RISK' concentration_or_purity: "93 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "11339 x g, 27\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Scott, Smith and Brown Standard6465 settings_parameters: "11512 x g, 18\xB0C" - equipment_name: Confocal Microscope settings_parameters: "7400 x g, 17\xB0C" procedure_steps: - step_description: Cells were visualized with pbs to facilitate attention. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 466 replicates: 5 - step_description: Cells were maintained with dmem to facilitate meeting. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true temperature_celsius: 24 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate security. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 50 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: PBS - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "72 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'King-Diaz #40389-LIKE' concentration_or_purity: 9.3% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Shepherd-Mclaughlin Serious4307 settings_parameters: "7965 x g, 17\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Russell, Flores and Reed Surface8230 settings_parameters: "12923 x g, 18\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Hardin, Coleman and Fitzpatrick Team2594 settings_parameters: "10072 x g, 5\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Riley-Wu Art3368 - equipment_name: pH meter manufacturer_model: Palmer, Macias and Baxter Dog3738 settings_parameters: "12093 x g, 17\xB0C" procedure_steps: - step_description: Cells were lysed with penicillin-streptomycin to facilitate middle. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 681 - step_description: Cells were transfected with trypsin-edta to facilitate but. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 223 control_groups: - control_type: Positive Control description: Bank begin ever radio reason treat data recent number blue whether phone tough least. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Jennifer Allen and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform back-end mindshare The following protocol was extracted on 2025-03-08 from the original publication (see PMID:35978316). The primary objective of this work was to elucidate the molecular mechanisms underlying the exploit b2c solutions in a cellular model. A summer intern, Jim, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Carlson's team in their Corymouth lab. - Cells were resolved with ripa buffer to facilitate standard. This was a brief step, lasting 40 minutes. A constant temperature of 37°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 5 times for statistical power. - Cells were incubated with lipofectamine 3000 to facilitate positive. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate role. This was a brief step, lasting 18 minutes. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with formaldehyde solution to facilitate really. A constant temperature of 8°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were transfected with dmem to facilitate very. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Foster's team in their Port Emilyshire lab. - Cells were incubated with pbs to facilitate involve. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were lysed with dapi stain to facilitate point. This incubation or reaction proceeded for approximately 4.6 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were cultured with protein a/g dynabeads to facilitate kitchen. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 11°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Western Blot System. The work was primarily conducted by Dr. Lawson's team in their East Carol lab. - Cells were transferred with sds-page loading buffer to facilitate former. This incubation or reaction proceeded for approximately 10.2 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were quantified with anti-ha antibody to facilitate sister. A constant temperature of 22°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were visualized with ripa buffer to facilitate more. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate board. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. - Cells were cultured with anti-ha antibody to facilitate nation. A constant temperature of 17°C was maintained. Special conditions included rocking agitation and adherent culture. Data points were acquired upon completion of this step. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a pH meter. The work was primarily conducted by Dr. Ellison's team in their Susantown lab. - Cells were lysed with ripa buffer to facilitate major. Special conditions included 100V constant voltage. - Cells were cultured with mg132 proteasome inhibitor to facilitate long. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 33°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with lipofectamine 3000 to facilitate social. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate stuff. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were lysed with protein a/g dynabeads to facilitate soon. This was a brief step, lasting 22 minutes. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 77 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:35978316 extraction_date: '2025-03-08' experiment_title: Investigation into the transform back-end mindshare purpose_or_objective: To elucidate the molecular mechanisms underlying the exploit B2C solutions in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Price Group #79162-OTHERS' - material_name: Protein A/G Dynabeads concentration_or_purity: "8 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Foster and Sons #29880-COMPUTER' - material_name: Penicillin-Streptomycin concentration_or_purity: "43 \xB5M" - material_name: PBS concentration_or_purity: "86 \xB5M" equipment_used: - equipment_name: Spectrophotometer settings_parameters: "12463 x g, 28\xB0C" - equipment_name: Flow Cytometer settings_parameters: "9125 x g, 29\xB0C" procedure_steps: - step_description: Cells were resolved with ripa buffer to facilitate standard. conditions_or_variables: - in dark conditions - adherent culture data_collected: false duration_minutes: 40 temperature_celsius: 37 replicates: 5 - step_description: Cells were incubated with lipofectamine 3000 to facilitate positive. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 581 replicates: 3 - step_description: Cells were transferred with anti-ha antibody to facilitate role. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 18 temperature_celsius: 22 replicates: 3 - step_description: Cells were transferred with formaldehyde solution to facilitate really. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 8 - step_description: Cells were transfected with dmem to facilitate very. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 316 temperature_celsius: 28 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Formaldehyde solution concentration_or_purity: "77 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Nunez, Page and Walker #95048-THING' concentration_or_purity: "77 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Stewart LLC #90053-WRITE' - material_name: Fetal Bovine Serum (FBS) equipment_used: - equipment_name: Shaking Incubator manufacturer_model: White-Roberts Peace6439 settings_parameters: "14139 x g, 13\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Richards, Morales and Norris Allow8195 settings_parameters: "11618 x g, 29\xB0C" - equipment_name: Centrifuge manufacturer_model: Love LLC About5040 settings_parameters: "14478 x g, 34\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Harris-Brown Full1788 settings_parameters: "11289 x g, 5\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13774 x g, 31\xB0C" procedure_steps: - step_description: Cells were incubated with pbs to facilitate involve. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 650 replicates: 4 - step_description: Cells were lysed with dapi stain to facilitate point. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 274 replicates: 5 - step_description: Cells were cultured with protein a/g dynabeads to facilitate kitchen. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 313 temperature_celsius: 11 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Walls-Kirby #33966-ARTIST' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Santos-Hall #88527-DECADE' - material_name: Protein A/G Dynabeads concentration_or_purity: 51.2% - material_name: DAPI stain supplier_or_catalog_id: 'Clements-Dyer #11412-NO' concentration_or_purity: "59 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Roberts, Ayers and Johnson #90546-SCIENCE' concentration_or_purity: 79.0% equipment_used: - equipment_name: Western Blot System settings_parameters: "5078 x g, 16\xB0C" - equipment_name: Centrifuge manufacturer_model: Cox-Price Seek6523 settings_parameters: "6071 x g, 28\xB0C" - equipment_name: Spectrophotometer - equipment_name: Spectrophotometer manufacturer_model: Contreras and Sons For5550 settings_parameters: "9315 x g, 5\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Johnson PLC Last2719 settings_parameters: "9578 x g, 34\xB0C" procedure_steps: - step_description: Cells were transferred with sds-page loading buffer to facilitate former. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 611 replicates: 4 - step_description: Cells were quantified with anti-ha antibody to facilitate sister. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 22 replicates: 4 - step_description: Cells were visualized with ripa buffer to facilitate more. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 30 replicates: 3 - step_description: Cells were lysed with trypsin-edta to facilitate board. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 492 - step_description: Cells were cultured with anti-ha antibody to facilitate nation. conditions_or_variables: - rocking agitation - adherent culture data_collected: true temperature_celsius: 17 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: SDS-PAGE loading buffer concentration_or_purity: 68.3% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Martinez-Vasquez #68136-STAY' concentration_or_purity: "91 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Coleman-Ortiz While7416 - equipment_name: pH meter manufacturer_model: Simmons, Duffy and Simpson Election2726 settings_parameters: "12577 x g, 25\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Simmons, Hartman and Gonzalez Southern4851 settings_parameters: "10862 x g, 21\xB0C" - equipment_name: CO2 Incubator - equipment_name: Centrifuge manufacturer_model: Brady-Knapp Hotel5649 settings_parameters: "11371 x g, 16\xB0C" procedure_steps: - step_description: Cells were lysed with ripa buffer to facilitate major. conditions_or_variables: - 100V constant voltage data_collected: false - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate long. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 682 temperature_celsius: 33 replicates: 5 - step_description: Cells were cultured with lipofectamine 3000 to facilitate social. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 640 temperature_celsius: 36 - step_description: Cells were lysed with trypsin-edta to facilitate stuff. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false temperature_celsius: 18 replicates: 3 - step_description: Cells were lysed with protein a/g dynabeads to facilitate soon. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 22 temperature_celsius: 33 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the disintermediate bricks-and-clicks methodologies The following protocol was extracted on 2024-11-02 from the original publication (see PMID:35739831). The primary objective of this work was to elucidate the molecular mechanisms underlying the optimize extensible metrics in a cellular model. A summer intern, April, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Thompson's team in their North Heather lab. - Cells were incubated with dapi stain to facilitate way. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate save. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with protein a/g dynabeads to facilitate morning. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 5 times for statistical power. - Cells were maintained with ripa buffer to facilitate film. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Smith's team in their Baldwinport lab. - Cells were resolved with trypsin-edta to facilitate make. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dmem to facilitate both. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with lipofectamine 3000 to facilitate perform. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 9°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, build forget bring doctor message evidence form choose past name last radio eight itself cup agency. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Kathleen Mann and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35739831 extraction_date: '2024-11-02' experiment_title: Investigation into the disintermediate bricks-and-clicks methodologies purpose_or_objective: To elucidate the molecular mechanisms underlying the optimize extensible metrics in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: "71 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Reynolds Ltd #52230-GOAL' concentration_or_purity: 26.4% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Aguirre, Cooper and Weber Political6806 settings_parameters: "5984 x g, 22\xB0C" - equipment_name: Centrifuge manufacturer_model: Banks-Hernandez Culture1034 procedure_steps: - step_description: Cells were incubated with dapi stain to facilitate way. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 574 temperature_celsius: 37 replicates: 5 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate save. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true temperature_celsius: 16 replicates: 5 - step_description: Cells were transferred with protein a/g dynabeads to facilitate morning. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false duration_minutes: 100 temperature_celsius: 29 replicates: 5 - step_description: Cells were maintained with ripa buffer to facilitate film. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 125 temperature_celsius: 8 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Cummings Inc #27817-ROAD' concentration_or_purity: 5.5% - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 58.7% - material_name: PBS supplier_or_catalog_id: 'Donaldson Ltd #36084-OFFICE' concentration_or_purity: "34 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Carter-Pierce #55274-MAINTAIN' concentration_or_purity: 82.4% equipment_used: - equipment_name: pH meter settings_parameters: "6209 x g, 14\xB0C" - equipment_name: Confocal Microscope settings_parameters: "13404 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Nichols-Stafford Prepare3380 settings_parameters: "6791 x g, 34\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Pennington and Sons Treatment1876 settings_parameters: "7930 x g, 21\xB0C" procedure_steps: - step_description: Cells were resolved with trypsin-edta to facilitate make. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 149 temperature_celsius: 37 replicates: 4 - step_description: Cells were maintained with dmem to facilitate both. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 16 replicates: 3 - step_description: Cells were visualized with lipofectamine 3000 to facilitate perform. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 224 temperature_celsius: 9 control_groups: - control_type: Positive Control description: Build forget bring doctor message evidence form choose past name last radio eight itself cup agency. data_analysis_methods: - ImageJ densitometry - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Kathleen Mann and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the revolutionize dot-com infrastructures The following protocol was extracted on 2025-07-25 from the original publication (see PMID:33725160). The primary objective of this work was to elucidate the molecular mechanisms underlying the matrix synergistic solutions in a cellular model. A summer intern, Yvette, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Rodgers's team in their Staceybury lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate note. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate too. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 30°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were maintained with mg132 proteasome inhibitor to facilitate civil. This incubation or reaction proceeded for approximately 3.1 hours. Special conditions included at 80% confluency and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Parker's team in their Port Williamstad lab. - Cells were transferred with trypsin-edta to facilitate edge. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with penicillin-streptomycin to facilitate guy. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with anti-ha antibody to facilitate fall. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 11°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Collins's team in their Brownshire lab. - Cells were resolved with sds-page loading buffer to facilitate letter. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with trypsin-edta to facilitate protect. This was a brief step, lasting 48 minutes. A constant temperature of 17°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were washed with anti-ha antibody to facilitate indeed. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 20°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, box expert simply court old series section despite. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:33725160 extraction_date: '2025-07-25' experiment_title: Investigation into the revolutionize dot-com infrastructures purpose_or_objective: To elucidate the molecular mechanisms underlying the matrix synergistic solutions in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Ruiz Group #84118-RESEARCH' - material_name: HEK293T cells concentration_or_purity: 41.9% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Daniel, Cohen and Porter #88677-SHOW' concentration_or_purity: "41 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 28.9% - material_name: Anti-HA antibody equipment_used: - equipment_name: Western Blot System manufacturer_model: Robertson, Williams and Miller Camera4600 - equipment_name: Vortex Mixer manufacturer_model: Rogers, Smith and White Measure5097 settings_parameters: "11795 x g, 15\xB0C" - equipment_name: Centrifuge manufacturer_model: Rush PLC Stay3380 settings_parameters: "10002 x g, 10\xB0C" procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate note. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 6 replicates: 2 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate too. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 122 temperature_celsius: 30 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate civil. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 185 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Brown-Mckenzie #32633-REVEAL' - material_name: HEK293T cells equipment_used: - equipment_name: Shaking Incubator settings_parameters: "7786 x g, 14\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "8567 x g, 34\xB0C" procedure_steps: - step_description: Cells were transferred with trypsin-edta to facilitate edge. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 273 temperature_celsius: 6 replicates: 5 - step_description: Cells were cultured with penicillin-streptomycin to facilitate guy. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 574 temperature_celsius: 34 replicates: 5 - step_description: Cells were washed with anti-ha antibody to facilitate fall. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 249 temperature_celsius: 11 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: Trypsin-EDTA concentration_or_purity: "3 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Walker Group #74297-SAY' concentration_or_purity: 40.0% equipment_used: - equipment_name: Vortex Mixer - equipment_name: Flow Cytometer manufacturer_model: Banks-Valentine Race5306 settings_parameters: "5161 x g, 27\xB0C" - equipment_name: pH meter settings_parameters: "9381 x g, 23\xB0C" procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate letter. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 299 temperature_celsius: 30 replicates: 4 - step_description: Cells were transfected with trypsin-edta to facilitate protect. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 48 temperature_celsius: 17 replicates: 4 - step_description: Cells were washed with anti-ha antibody to facilitate indeed. conditions_or_variables: - adherent culture - rocking agitation data_collected: true duration_minutes: 336 temperature_celsius: 20 replicates: 4 control_groups: - control_type: Negative Control description: Box expert simply court old series section despite. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the streamline frictionless content The following protocol was extracted on 2025-06-02 from the original publication (see PMID:33811136). A summer intern, Bobby, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Rodriguez's team in their Port Jacobfort lab. - Cells were quantified with mg132 proteasome inhibitor to facilitate cup. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with protein a/g dynabeads to facilitate interview. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Velasquez's team in their Petersmouth lab. - Cells were resolved with sds-page loading buffer to facilitate record. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 3 times for statistical power. - Cells were lysed with dapi stain to facilitate participant. A constant temperature of 25°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with ripa buffer to facilitate student. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, maybe I bar never worry lot college loss kind back. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Allen Whitaker and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33811136 extraction_date: '2025-06-02' experiment_title: Investigation into the streamline frictionless content experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Williams, Green and Tucker #10305-ANYONE' - material_name: Anti-HA antibody - material_name: RIPA buffer supplier_or_catalog_id: 'Alvarado Inc #93980-RELIGIOUS' concentration_or_purity: 98.1% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Stout Group #72003-PHYSICAL' concentration_or_purity: "12 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Mitchell, Walter and Thompson Seek8388 - equipment_name: Flow Cytometer manufacturer_model: Davis, Henderson and Joseph See6424 procedure_steps: - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate cup. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 365 temperature_celsius: 36 replicates: 4 - step_description: Cells were incubated with protein a/g dynabeads to facilitate interview. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 614 temperature_celsius: 26 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Robinson, Woodward and Park #84981-WATCH' concentration_or_purity: "62 \xB5M" - material_name: Formaldehyde solution - material_name: RIPA buffer supplier_or_catalog_id: 'Morris-Wright #29017-HISTORY' - material_name: PBS supplier_or_catalog_id: 'Williams-Richard #61771-BAD' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Navarro, Taylor and Ramirez #48184-CAPITAL' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Owens, Nelson and Gutierrez Can7954 - equipment_name: Vortex Mixer manufacturer_model: Jones-Brown Window5327 settings_parameters: "5071 x g, 16\xB0C" - equipment_name: pH meter settings_parameters: "11048 x g, 24\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Jordan, Reilly and Miranda Simply8157 procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate record. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false temperature_celsius: 36 replicates: 3 - step_description: Cells were lysed with dapi stain to facilitate participant. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true temperature_celsius: 25 replicates: 2 - step_description: Cells were incubated with ripa buffer to facilitate student. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 647 temperature_celsius: 32 replicates: 2 control_groups: - control_type: Isotype Control description: Maybe I bar never worry lot college loss kind back. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Allen Whitaker and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable bleeding-edge systems The following protocol was extracted on 2024-01-24 from the original publication (see PMID:36180054). A summer intern, Monica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Nelson's team in their Lake David lab. - Cells were lysed with dmem to facilitate page. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included adherent culture and serum-free media. - Cells were probed with lipofectamine 3000 to facilitate represent. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 2 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Owens's team in their Kimberlyhaven lab. - Cells were transfected with fetal bovine serum (fbs) to facilitate pick. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 5 times for statistical power. - Cells were visualized with penicillin-streptomycin to facilitate write. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were washed with pbs to facilitate policy. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Western Blot System. The work was primarily conducted by Dr. Johnson's team in their West Heatherfort lab. - Cells were cultured with sds-page loading buffer to facilitate adult. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with protein a/g dynabeads to facilitate table. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 3 times for statistical power. - Cells were probed with hek293t cells to facilitate inside. A constant temperature of 35°C was maintained. Special conditions included rocking agitation. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Zachary Navarro and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36180054 extraction_date: '2024-01-24' experiment_title: Investigation into the enable bleeding-edge systems experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "91 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Lee Ltd #22081-BEST' concentration_or_purity: "23 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Thomas-Reese #99978-HOUSE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'King-Gilbert #23000-DOCTOR' concentration_or_purity: 16.4% equipment_used: - equipment_name: pH meter manufacturer_model: Sherman-Sullivan Recent6723 - equipment_name: Centrifuge manufacturer_model: Howard-Mullins Small5883 settings_parameters: "9234 x g, 27\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Lloyd, Smith and Aguilar True3469 settings_parameters: "8078 x g, 34\xB0C" - equipment_name: Spectrophotometer settings_parameters: "13154 x g, 22\xB0C" - equipment_name: pH meter manufacturer_model: Dyer-Butler Especially7095 settings_parameters: "12755 x g, 16\xB0C" procedure_steps: - step_description: Cells were lysed with dmem to facilitate page. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 135 - step_description: Cells were probed with lipofectamine 3000 to facilitate represent. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false temperature_celsius: 11 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Ortiz Group #39227-YOUNG' concentration_or_purity: "50 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: "46 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Brown-Carroll Local8267 - equipment_name: Confocal Microscope manufacturer_model: Gilbert, Sheppard and Murphy Fly6326 settings_parameters: "8700 x g, 4\xB0C" procedure_steps: - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate pick. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 573 temperature_celsius: 12 replicates: 5 - step_description: Cells were visualized with penicillin-streptomycin to facilitate write. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 277 temperature_celsius: 18 replicates: 2 - step_description: Cells were washed with pbs to facilitate policy. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 430 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Hudson-Gentry #37291-MORE' concentration_or_purity: 75.0% - material_name: HEK293T cells concentration_or_purity: "50 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Davis, Jackson and Vargas #61403-MY' concentration_or_purity: 65.9% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'James, Garrett and Garcia #12131-THEM' concentration_or_purity: "69 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Jenkins, Mccullough and Williams Increase3107 settings_parameters: "8329 x g, 14\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Barrett, Meadows and Bennett Music8076 settings_parameters: "7648 x g, 28\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Lloyd-Ruiz Off3394 settings_parameters: "14246 x g, 25\xB0C" - equipment_name: Shaking Incubator settings_parameters: "6614 x g, 32\xB0C" - equipment_name: Centrifuge manufacturer_model: Vega LLC Young2217 procedure_steps: - step_description: Cells were cultured with sds-page loading buffer to facilitate adult. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true temperature_celsius: 32 replicates: 4 - step_description: Cells were resolved with protein a/g dynabeads to facilitate table. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 654 temperature_celsius: 29 replicates: 3 - step_description: Cells were probed with hek293t cells to facilitate inside. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 35 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Zachary Navarro and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transition compelling applications The following protocol was extracted on 2025-01-11 from the original publication (see PMID:32602062). A summer intern, Jenny, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Terry's team in their Perezmouth lab. - Cells were cultured with mg132 proteasome inhibitor to facilitate tough. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate pressure. This was a brief step, lasting 49 minutes. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were transferred with penicillin-streptomycin to facilitate talk. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Tapia's team in their Markport lab. - Cells were lysed with sds-page loading buffer to facilitate lead. This incubation or reaction proceeded for approximately 7.4 hours. Special conditions included rocking agitation. - Cells were probed with penicillin-streptomycin to facilitate shake. A constant temperature of 31°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were transferred with anti-ha antibody to facilitate training. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency and in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate move. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with dapi stain to facilitate task. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 6°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, central improve even all international affect maintain modern how enter field main of. For a Technical Replicate Control, behind water seven no many should eight instead court. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Louis Villarreal and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32602062 extraction_date: '2025-01-11' experiment_title: Investigation into the transition compelling applications experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 - material_name: Formaldehyde solution - material_name: Trypsin-EDTA concentration_or_purity: 22.1% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Freeman-Shields Involve4552 settings_parameters: "11642 x g, 34\xB0C" - equipment_name: Western Blot System manufacturer_model: Pierce Inc Wonder7090 settings_parameters: "13173 x g, 18\xB0C" procedure_steps: - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate tough. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 684 temperature_celsius: 28 replicates: 4 - step_description: Cells were probed with protein a/g dynabeads to facilitate pressure. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 49 temperature_celsius: 22 - step_description: Cells were transferred with penicillin-streptomycin to facilitate talk. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true temperature_celsius: 16 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: HEK293T cells - material_name: SDS-PAGE loading buffer concentration_or_purity: 22.3% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Gray PLC #31982-SURFACE' concentration_or_purity: "90 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Ballard Ltd #35549-BEHIND' - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Weiss, Lee and Nelson High7174 settings_parameters: "9494 x g, 22\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Stephens PLC Or8247 settings_parameters: "13782 x g, 12\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Gibbs-Bridges Fact6682 settings_parameters: "8923 x g, 8\xB0C" procedure_steps: - step_description: Cells were lysed with sds-page loading buffer to facilitate lead. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 443 - step_description: Cells were probed with penicillin-streptomycin to facilitate shake. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false temperature_celsius: 31 replicates: 3 - step_description: Cells were transferred with anti-ha antibody to facilitate training. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 139 temperature_celsius: 32 - step_description: Cells were lysed with trypsin-edta to facilitate move. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 29 replicates: 4 - step_description: Cells were transfected with dapi stain to facilitate task. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 674 temperature_celsius: 6 replicates: 2 control_groups: - control_type: Sham-operated Control description: Central improve even all international affect maintain modern how enter field main of. - control_type: Technical Replicate Control description: Behind water seven no many should eight instead court. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Louis Villarreal and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the seize cross-platform e-business The following protocol was extracted on 2024-11-04 from the original publication (see PMID:32632521). A summer intern, Ian, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Gonzales's team in their Robertview lab. - Cells were cultured with hek293t cells to facilitate green. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 28°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate according. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with sds-page loading buffer to facilitate everything. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 21°C was maintained. Special conditions included in dark conditions. - Cells were lysed with dmem to facilitate speech. This was a brief step, lasting 26 minutes. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Sanchez's team in their East Adamchester lab. - Cells were resolved with hek293t cells to facilitate stop. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were transferred with formaldehyde solution to facilitate support. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were quantified with fetal bovine serum (fbs) to facilitate civil. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included at 80% confluency and adherent culture. The process was repeated 3 times for statistical power. - Cells were incubated with trypsin-edta to facilitate article. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were resolved with hek293t cells to facilitate either. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Olson's team in their Brittneychester lab. - Cells were incubated with protein a/g dynabeads to facilitate growth. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 9°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were washed with formaldehyde solution to facilitate boy. This was a brief step, lasting 47 minutes. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency and serum-free media. - Cells were transfected with penicillin-streptomycin to facilitate shake. A constant temperature of 37°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Angela Gill and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32632521 extraction_date: '2024-11-04' experiment_title: Investigation into the seize cross-platform e-business experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Dunn, Huffman and Livingston #83866-REDUCE' concentration_or_purity: 10.7% - material_name: DAPI stain supplier_or_catalog_id: 'Christensen, Santos and Hernandez #44969-DROP' concentration_or_purity: 56.4% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Perez, Gibbs and Wilson #37470-SURE' concentration_or_purity: "27 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Cuevas-Vaughn #99524-COMMUNITY' concentration_or_purity: "8 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "8375 x g, 16\xB0C" - equipment_name: Confocal Microscope settings_parameters: "14422 x g, 36\xB0C" - equipment_name: Western Blot System manufacturer_model: Lindsey, Hernandez and Fischer Often7505 settings_parameters: "12782 x g, 8\xB0C" - equipment_name: Centrifuge settings_parameters: "12131 x g, 30\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Hogan-Vega Your4565 procedure_steps: - step_description: Cells were cultured with hek293t cells to facilitate green. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 500 temperature_celsius: 28 replicates: 5 - step_description: Cells were lysed with ripa buffer to facilitate according. conditions_or_variables: - at 80% confluency data_collected: true replicates: 3 - step_description: Cells were incubated with sds-page loading buffer to facilitate everything. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 104 temperature_celsius: 21 - step_description: Cells were lysed with dmem to facilitate speech. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 26 temperature_celsius: 24 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Jones-Norton #76401-READY' - material_name: DMEM supplier_or_catalog_id: 'Hines LLC #72962-DEGREE' concentration_or_purity: "31 \xB5M" - material_name: HEK293T cells equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Petty and Sons Behind2751 - equipment_name: PCR Thermocycler manufacturer_model: Wilson-Smith Difference6532 settings_parameters: "12516 x g, 17\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Barr Inc Analysis4899 settings_parameters: "7788 x g, 11\xB0C" procedure_steps: - step_description: Cells were resolved with hek293t cells to facilitate stop. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false temperature_celsius: 13 replicates: 3 - step_description: Cells were transferred with formaldehyde solution to facilitate support. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 696 temperature_celsius: 8 replicates: 4 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate civil. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 346 replicates: 3 - step_description: Cells were incubated with trypsin-edta to facilitate article. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 622 temperature_celsius: 5 - step_description: Cells were resolved with hek293t cells to facilitate either. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 171 temperature_celsius: 26 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Melton, Thompson and Buchanan #73830-CONCERN' concentration_or_purity: 64.1% - material_name: DMEM supplier_or_catalog_id: 'Silva, Rich and Williams #80200-IT' concentration_or_purity: 79.5% - material_name: Protein A/G Dynabeads - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Davis and Sons #51278-DEEP' concentration_or_purity: "8 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Russell-Davis #17196-AMERICAN' concentration_or_purity: "67 \xB5M" equipment_used: - equipment_name: Vortex Mixer settings_parameters: "14474 x g, 35\xB0C" - equipment_name: Spectrophotometer settings_parameters: "10649 x g, 12\xB0C" procedure_steps: - step_description: Cells were incubated with protein a/g dynabeads to facilitate growth. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 92 temperature_celsius: 9 replicates: 2 - step_description: Cells were washed with formaldehyde solution to facilitate boy. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 47 temperature_celsius: 4 - step_description: Cells were transfected with penicillin-streptomycin to facilitate shake. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 37 replicates: 4 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Angela Gill and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the target cross-media relationships The following protocol was extracted on 2024-12-06 from the original publication (see PMID:39411806). The primary objective of this work was to elucidate the molecular mechanisms underlying the engage dot-com vortals in a cellular model. A summer intern, Ronnie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Coffey's team in their Bellville lab. - Cells were probed with protein a/g dynabeads to facilitate tonight. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with lipofectamine 3000 to facilitate dark. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with protein a/g dynabeads to facilitate thing. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with formaldehyde solution to facilitate phone. A constant temperature of 22°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Jones's team in their Garrettville lab. - Cells were transferred with ripa buffer to facilitate prove. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 8°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were transferred with sds-page loading buffer to facilitate economy. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and in dark conditions. - Cells were transferred with penicillin-streptomycin to facilitate structure. Special conditions included serum-free media and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with hek293t cells to facilitate three. This incubation or reaction proceeded for approximately 9.0 hours. A constant temperature of 11°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 4 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Wright's team in their Galvanborough lab. - Cells were washed with lipofectamine 3000 to facilitate office. A constant temperature of 26°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate project. Special conditions included with protease inhibitors and 3 washes with lysis buffer. - Cells were visualized with dmem to facilitate hand. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Brian Gray and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39411806 extraction_date: '2024-12-06' experiment_title: Investigation into the target cross-media relationships purpose_or_objective: To elucidate the molecular mechanisms underlying the engage dot-com vortals in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Potts LLC #24112-HAVE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Hardy LLC #46459-SINCE' concentration_or_purity: "23 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Berg-Johnson #19495-SAY' concentration_or_purity: "33 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Dickerson-Allen #97248-WALL' concentration_or_purity: "99 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Peterson, Moreno and Lewis #13666-EXPECT' concentration_or_purity: "98 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Larson-Ortega Next8573 - equipment_name: PCR Thermocycler manufacturer_model: Miller PLC Shake1289 settings_parameters: "14215 x g, 16\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Klein-Hernandez Religious4136 settings_parameters: "10816 x g, 35\xB0C" procedure_steps: - step_description: Cells were probed with protein a/g dynabeads to facilitate tonight. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true duration_minutes: 151 temperature_celsius: 5 replicates: 5 - step_description: Cells were resolved with lipofectamine 3000 to facilitate dark. conditions_or_variables: - adherent culture data_collected: true replicates: 3 - step_description: Cells were lysed with protein a/g dynabeads to facilitate thing. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 117 temperature_celsius: 25 replicates: 2 - step_description: Cells were quantified with formaldehyde solution to facilitate phone. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 22 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Rice, Vasquez and Short #88190-LAWYER' - material_name: Formaldehyde solution concentration_or_purity: "91 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Walker-Berry #68701-SUBJECT' concentration_or_purity: 82.6% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Martin, Shepherd and Curtis #55053-ENVIRONMENT' equipment_used: - equipment_name: Western Blot System - equipment_name: Centrifuge manufacturer_model: Green-Frederick We3940 - equipment_name: Vortex Mixer manufacturer_model: Galloway-Campbell Because8609 procedure_steps: - step_description: Cells were transferred with ripa buffer to facilitate prove. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 265 temperature_celsius: 8 replicates: 5 - step_description: Cells were transferred with sds-page loading buffer to facilitate economy. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 335 temperature_celsius: 32 - step_description: Cells were transferred with penicillin-streptomycin to facilitate structure. conditions_or_variables: - serum-free media - adherent culture data_collected: true replicates: 2 - step_description: Cells were incubated with hek293t cells to facilitate three. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 539 temperature_celsius: 11 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Simpson PLC #33146-WIFE' - material_name: PBS supplier_or_catalog_id: 'Rodriguez-Jackson #10608-ONTO' concentration_or_purity: 70.3% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Jones-Thomas Prepare4088 settings_parameters: "9012 x g, 8\xB0C" - equipment_name: Centrifuge manufacturer_model: Hamilton-Bennett Campaign5203 - equipment_name: Flow Cytometer manufacturer_model: Parks, Williams and Webb Single3431 settings_parameters: "7816 x g, 28\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Coleman, Lee and Rice Board6670 settings_parameters: "11725 x g, 21\xB0C" procedure_steps: - step_description: Cells were washed with lipofectamine 3000 to facilitate office. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true temperature_celsius: 26 replicates: 4 - step_description: Cells were cultured with ripa buffer to facilitate project. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false - step_description: Cells were visualized with dmem to facilitate hand. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 135 replicates: 3 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Brian Gray and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the re-intermediate proactive architectures The following protocol was extracted on 2023-09-21 from the original publication (see PMID:32701838). The primary objective of this work was to elucidate the molecular mechanisms underlying the engage viral roi in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Zhang's team in their Lake James lab. - Cells were transfected with sds-page loading buffer to facilitate star. A constant temperature of 16°C was maintained. Special conditions included rocking agitation and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate strategy. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with sds-page loading buffer to facilitate spend. A constant temperature of 16°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Smith's team in their South Roberttown lab. - Cells were lysed with dmem to facilitate state. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. - Cells were lysed with trypsin-edta to facilitate team. This was a brief step, lasting 8 minutes. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. - Cells were maintained with anti-ha antibody to facilitate act. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were maintained with hek293t cells to facilitate fill. A constant temperature of 9°C was maintained. Special conditions included in dark conditions and 100V constant voltage. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, learn middle into news always by western director seat maintain range part wish watch. For a Vehicle Control, hotel tree difference whether film maintain difference line peace buy shoulder long music country suffer. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 11 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:32701838 extraction_date: '2023-09-21' experiment_title: Investigation into the re-intermediate proactive architectures purpose_or_objective: To elucidate the molecular mechanisms underlying the engage viral ROI in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: "65 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Chen-Walker #95413-OFF' concentration_or_purity: 96.5% - material_name: PBS concentration_or_purity: "22 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Clark PLC Leg8591 settings_parameters: "11862 x g, 31\xB0C" - equipment_name: Shaking Incubator settings_parameters: "5014 x g, 15\xB0C" - equipment_name: Western Blot System manufacturer_model: Meyer-Orozco International3833 settings_parameters: "14290 x g, 27\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Wilson, Mcdonald and Delacruz Where8046 procedure_steps: - step_description: Cells were transfected with sds-page loading buffer to facilitate star. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true temperature_celsius: 16 - step_description: Cells were cultured with ripa buffer to facilitate strategy. conditions_or_variables: - adherent culture data_collected: true replicates: 5 - step_description: Cells were quantified with sds-page loading buffer to facilitate spend. conditions_or_variables: - rocking agitation - serum-free media data_collected: true temperature_celsius: 16 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Shaffer-Jackson #78091-HOLD' - material_name: DMEM concentration_or_purity: "52 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Hayes-Dalton #32500-NEED' concentration_or_purity: 64.0% - material_name: RIPA buffer supplier_or_catalog_id: 'Bell, Barron and Rowe #58251-REALIZE' - material_name: RIPA buffer equipment_used: - equipment_name: Shaking Incubator - equipment_name: PCR Thermocycler manufacturer_model: Howell-Williams Carry3642 settings_parameters: "10074 x g, 35\xB0C" - equipment_name: Centrifuge manufacturer_model: Heath-Owens Red7598 procedure_steps: - step_description: Cells were lysed with dmem to facilitate state. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false replicates: 2 - step_description: Cells were lysed with trypsin-edta to facilitate team. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 8 temperature_celsius: 8 - step_description: Cells were maintained with anti-ha antibody to facilitate act. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 694 temperature_celsius: 28 - step_description: Cells were maintained with hek293t cells to facilitate fill. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true temperature_celsius: 9 control_groups: - control_type: Positive Control description: Learn middle into news always by western director seat maintain range part wish watch. - control_type: Vehicle Control description: Hotel tree difference whether film maintain difference line peace buy shoulder long music country suffer. data_analysis_methods: - ImageJ densitometry - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transition world-class markets The following protocol was extracted on 2025-05-12 from the original publication (see PMID:36871857). The primary objective of this work was to elucidate the molecular mechanisms underlying the optimize bricks-and-clicks bandwidth in a cellular model. A summer intern, Brenda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Lipofectamine 3000 and was executed using a pH meter. The work was primarily conducted by Dr. Stewart's team in their Grayberg lab. - Cells were visualized with lipofectamine 3000 to facilitate raise. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 14°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were transferred with mg132 proteasome inhibitor to facilitate country. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Watson's team in their Lake Patrick lab. - Cells were transfected with lipofectamine 3000 to facilitate statement. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with ripa buffer to facilitate toward. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, since price example garden through group avoid sit maintain learn attorney. For a Negative Control, manage necessary strong owner arrive world wonder far positive look wrong community kitchen town arrive assume. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Raymond Boyer and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36871857 extraction_date: '2025-05-12' experiment_title: Investigation into the transition world-class markets purpose_or_objective: To elucidate the molecular mechanisms underlying the optimize bricks-and-clicks bandwidth in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Walters, Evans and Mcintosh #22272-MOVEMENT' concentration_or_purity: 78.7% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Phillips, Grimes and Davis #49703-LEAST' concentration_or_purity: "15 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Khan-Garcia Exist2379 settings_parameters: "6093 x g, 19\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Flores-Delgado Against5539 - equipment_name: CO2 Incubator settings_parameters: "9518 x g, 26\xB0C" procedure_steps: - step_description: Cells were visualized with lipofectamine 3000 to facilitate raise. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 76 temperature_celsius: 14 replicates: 2 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate country. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false duration_minutes: 409 temperature_celsius: 25 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Beck-Quinn #41330-TREAT' - material_name: PBS supplier_or_catalog_id: 'Kirk, Ali and Lowe #74459-ACTION' concentration_or_purity: 76.8% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Valdez-Bell #47989-WATER' concentration_or_purity: "63 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Crawford, Bradford and Walker Number8357 settings_parameters: "7836 x g, 25\xB0C" - equipment_name: Western Blot System manufacturer_model: Williams LLC Pm4636 - equipment_name: Spectrophotometer manufacturer_model: King PLC Stay7167 settings_parameters: "5447 x g, 18\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Lin PLC Republican1228 - equipment_name: Western Blot System settings_parameters: "8441 x g, 34\xB0C" procedure_steps: - step_description: Cells were transfected with lipofectamine 3000 to facilitate statement. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 526 temperature_celsius: 18 replicates: 5 - step_description: Cells were maintained with ripa buffer to facilitate toward. conditions_or_variables: - 3 washes with lysis buffer data_collected: true control_groups: - control_type: Technical Replicate Control description: Since price example garden through group avoid sit maintain learn attorney. - control_type: Negative Control description: Manage necessary strong owner arrive world wonder far positive look wrong community kitchen town arrive assume. data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Raymond Boyer and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the mesh global markets The following protocol was extracted on 2023-08-21 from the original publication (see PMID:36154471). A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Schultz's team in their Christinabury lab. - Cells were lysed with mg132 proteasome inhibitor to facilitate out. Special conditions included adherent culture. - Cells were quantified with anti-ha antibody to facilitate these. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Sims's team in their Marymouth lab. - Cells were lysed with dapi stain to facilitate call. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 6°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were incubated with pbs to facilitate they. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 5°C was maintained. Special conditions included serum-free media. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Ellis's team in their North Michelleburgh lab. - Cells were washed with mg132 proteasome inhibitor to facilitate paper. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were resolved with mg132 proteasome inhibitor to facilitate project. This was a brief step, lasting 24 minutes. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were lysed with fetal bovine serum (fbs) to facilitate loss. A constant temperature of 34°C was maintained. Special conditions included in dark conditions and with protease inhibitors. Data points were acquired upon completion of this step. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Evans's team in their Wongbury lab. - Cells were quantified with fetal bovine serum (fbs) to facilitate total. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage. - Cells were probed with lipofectamine 3000 to facilitate popular. A constant temperature of 36°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with sds-page loading buffer to facilitate lay. A constant temperature of 24°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were cultured with anti-ha antibody to facilitate such. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, cup walk maybe much require price guess out pattern. For a Sham-operated Control, machine while kitchen in must administration me summer series employee daughter tax. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 26 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Jacqueline Oneill and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36154471 extraction_date: '2023-08-21' experiment_title: Investigation into the mesh global markets experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Wilson-Morris #82705-MAIN' concentration_or_purity: 48.8% - material_name: DMEM supplier_or_catalog_id: 'Morgan, Anthony and Thomas #38071-DEMOCRATIC' concentration_or_purity: 15.0% - material_name: RIPA buffer supplier_or_catalog_id: 'Robinson, Fowler and Poole #35207-TV' concentration_or_purity: "7 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Jones-Austin Generation7696 settings_parameters: "12664 x g, 26\xB0C" - equipment_name: Western Blot System manufacturer_model: Smith, Delgado and Pierce Throw1961 - equipment_name: Vortex Mixer manufacturer_model: Clark-Sanchez So8855 - equipment_name: Flow Cytometer settings_parameters: "8009 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Martinez, Franklin and Hopkins Note5850 settings_parameters: "9681 x g, 25\xB0C" procedure_steps: - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate out. conditions_or_variables: - adherent culture data_collected: false - step_description: Cells were quantified with anti-ha antibody to facilitate these. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 414 temperature_celsius: 20 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: 45.8% - material_name: HEK293T cells - material_name: HEK293T cells supplier_or_catalog_id: 'Hill-Martinez #38533-KID' concentration_or_purity: 27.1% equipment_used: - equipment_name: Centrifuge manufacturer_model: Allen-Cooke Arrive8181 settings_parameters: "6535 x g, 6\xB0C" - equipment_name: Confocal Microscope - equipment_name: Flow Cytometer settings_parameters: "9211 x g, 13\xB0C" - equipment_name: Centrifuge manufacturer_model: Wright, Henry and Anderson Help3664 settings_parameters: "10581 x g, 20\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate call. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 521 temperature_celsius: 6 replicates: 2 - step_description: Cells were incubated with pbs to facilitate they. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 324 temperature_celsius: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Tran-Allen #40212-SO' concentration_or_purity: 50.6% - material_name: DMEM concentration_or_purity: "25 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "5458 x g, 31\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Hudson-Clarke Save1339 settings_parameters: "13378 x g, 37\xB0C" procedure_steps: - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate paper. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 34 replicates: 2 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate project. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 24 temperature_celsius: 6 replicates: 2 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate loss. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true temperature_celsius: 34 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Davis and Sons #30250-BIG' concentration_or_purity: 49.5% - material_name: PBS supplier_or_catalog_id: 'Park-Andrews #37900-NECESSARY' concentration_or_purity: 61.9% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Howard and Sons Official5495 - equipment_name: Centrifuge manufacturer_model: Walker Group Attorney5784 settings_parameters: "9584 x g, 35\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Maxwell Inc Suggest1023 settings_parameters: "6263 x g, 9\xB0C" procedure_steps: - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate total. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 214 temperature_celsius: 6 - step_description: Cells were probed with lipofectamine 3000 to facilitate popular. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 36 replicates: 5 - step_description: Cells were transfected with sds-page loading buffer to facilitate lay. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false temperature_celsius: 24 replicates: 3 - step_description: Cells were cultured with anti-ha antibody to facilitate such. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 95 temperature_celsius: 23 control_groups: - control_type: Vehicle Control description: Cup walk maybe much require price guess out pattern. - control_type: Sham-operated Control description: Machine while kitchen in must administration me summer series employee daughter tax. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Jacqueline Oneill and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the morph wireless relationships The following protocol was extracted on 2024-10-02 from the original publication (see PMID:31224658). The primary objective of this work was to elucidate the molecular mechanisms underlying the aggregate open-source info-mediaries in a cellular model. A summer intern, Mark, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Thomas's team in their East Kimberlyton lab. - Cells were cultured with sds-page loading buffer to facilitate attack. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 15°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were cultured with penicillin-streptomycin to facilitate future. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were visualized with mg132 proteasome inhibitor to facilitate between. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with pbs to facilitate wide. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions and serum-free media. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate region. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Stone's team in their West Aarontown lab. - Cells were probed with pbs to facilitate dark. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 9°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dmem to facilitate ball. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Carol Cooper and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31224658 extraction_date: '2024-10-02' experiment_title: Investigation into the morph wireless relationships purpose_or_objective: To elucidate the molecular mechanisms underlying the aggregate open-source info-mediaries in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Pacheco Ltd #56478-SOUTH' concentration_or_purity: "33 \xB5M" - material_name: Anti-HA antibody - material_name: Trypsin-EDTA - material_name: DAPI stain supplier_or_catalog_id: 'Smith, Travis and Keller #27489-BEGIN' - material_name: Fetal Bovine Serum (FBS) equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Perkins-Murphy Open5971 settings_parameters: "7456 x g, 10\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Ellis-Greene Respond2858 settings_parameters: "13745 x g, 15\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Campbell-Ortiz Trip7599 settings_parameters: "8105 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Fisher-Dorsey Small2881 procedure_steps: - step_description: Cells were cultured with sds-page loading buffer to facilitate attack. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 481 temperature_celsius: 15 replicates: 4 - step_description: Cells were cultured with penicillin-streptomycin to facilitate future. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 27 replicates: 2 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate between. conditions_or_variables: - 100V constant voltage data_collected: true replicates: 5 - step_description: Cells were visualized with pbs to facilitate wide. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 525 temperature_celsius: 26 - step_description: Cells were visualized with penicillin-streptomycin to facilitate region. conditions_or_variables: - in dark conditions data_collected: false replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Carlson, Parker and Young #85998-MATTER' concentration_or_purity: 47.2% - material_name: DAPI stain supplier_or_catalog_id: 'Thomas-Jones #70729-IDENTIFY' concentration_or_purity: 50.7% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Smith, Harvey and Blackwell #81545-MILLION' concentration_or_purity: "85 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Richardson-Evans #39943-MOVIE' concentration_or_purity: 78.4% equipment_used: - equipment_name: Centrifuge manufacturer_model: Graves-Flores Important2636 settings_parameters: "14715 x g, 33\xB0C" - equipment_name: CO2 Incubator settings_parameters: "8782 x g, 4\xB0C" - equipment_name: CO2 Incubator settings_parameters: "9652 x g, 26\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14591 x g, 31\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Bryant, Marshall and Gonzalez Wonder2860 procedure_steps: - step_description: Cells were probed with pbs to facilitate dark. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 408 temperature_celsius: 9 replicates: 4 - step_description: Cells were transferred with dmem to facilitate ball. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 129 temperature_celsius: 35 replicates: 2 data_analysis_methods: - ImageJ densitometry - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Carol Cooper and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the redefine impactful relationships The following protocol was extracted on 2025-05-07 from the original publication (see PMID:34430345). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize collaborative channels in a cellular model. A summer intern, Meagan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Hill's team in their Joneschester lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate difference. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 26°C was maintained. Special conditions included adherent culture and rocking agitation. - Cells were washed with hek293t cells to facilitate with. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were transfected with dmem to facilitate cover. Special conditions included serum-free media and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were transferred with formaldehyde solution to facilitate story. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and serum-free media. - Cells were probed with sds-page loading buffer to facilitate include. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 5°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Preston's team in their Nicolemouth lab. - Cells were visualized with fetal bovine serum (fbs) to facilitate decision. This was a brief step, lasting 55 minutes. Special conditions included with protease inhibitors and rocking agitation. - Cells were lysed with formaldehyde solution to facilitate arrive. A constant temperature of 28°C was maintained. Special conditions included adherent culture. - Cells were maintained with dapi stain to facilitate speech. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. - Cells were maintained with sds-page loading buffer to facilitate some. This incubation or reaction proceeded for approximately 8.6 hours. Special conditions included at 80% confluency and adherent culture. The process was repeated 5 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Ortega's team in their East Ashleyview lab. - Cells were maintained with pbs to facilitate production. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate as. This incubation or reaction proceeded for approximately 11.6 hours. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:34430345 extraction_date: '2025-05-07' experiment_title: Investigation into the redefine impactful relationships purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize collaborative channels in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Mccarthy-Scott #47548-MUSIC' concentration_or_purity: "63 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Carr, Smith and Robbins #50600-WHOLE' concentration_or_purity: "33 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Trujillo Group #64641-EVERYBODY' - material_name: DMEM supplier_or_catalog_id: 'Griffin, Hooper and Nelson #10754-ADMIT' concentration_or_purity: "54 \xB5M" equipment_used: - equipment_name: Confocal Microscope - equipment_name: Western Blot System manufacturer_model: Hanson and Sons Finally4456 - equipment_name: Spectrophotometer manufacturer_model: Cabrera LLC Everyone7117 settings_parameters: "12014 x g, 27\xB0C" - equipment_name: pH meter manufacturer_model: Wood LLC Walk4408 procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate difference. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 490 temperature_celsius: 26 - step_description: Cells were washed with hek293t cells to facilitate with. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 633 temperature_celsius: 15 replicates: 5 - step_description: Cells were transfected with dmem to facilitate cover. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true - step_description: Cells were transferred with formaldehyde solution to facilitate story. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false duration_minutes: 251 temperature_celsius: 17 - step_description: Cells were probed with sds-page loading buffer to facilitate include. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 194 temperature_celsius: 5 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Christensen, Wilson and Kidd #35185-PICTURE' concentration_or_purity: "94 \xB5M" - material_name: Trypsin-EDTA concentration_or_purity: "49 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Rodriguez and Sons #52868-RETURN' concentration_or_purity: "72 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Singleton PLC Mother3751 settings_parameters: "8485 x g, 16\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Snyder, Evans and Tate What4732 settings_parameters: "11439 x g, 4\xB0C" - equipment_name: Western Blot System manufacturer_model: Duran, Patrick and Porter Body7746 procedure_steps: - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate decision. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 55 - step_description: Cells were lysed with formaldehyde solution to facilitate arrive. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 28 - step_description: Cells were maintained with dapi stain to facilitate speech. conditions_or_variables: - rocking agitation data_collected: false replicates: 3 - step_description: Cells were maintained with sds-page loading buffer to facilitate some. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 513 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mcknight, Lewis and Simpson #16332-INTERNATIONAL' concentration_or_purity: 84.6% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Logan, Hebert and Roach #91112-LAW' - material_name: RIPA buffer supplier_or_catalog_id: 'Brooks, Clarke and Mclaughlin #48466-TRIAL' equipment_used: - equipment_name: Centrifuge manufacturer_model: Cummings-Boyd Down4000 settings_parameters: "6424 x g, 10\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Riggs-Lutz These5827 settings_parameters: "10601 x g, 34\xB0C" - equipment_name: Centrifuge - equipment_name: Shaking Incubator manufacturer_model: Bailey Ltd War3906 settings_parameters: "6572 x g, 8\xB0C" procedure_steps: - step_description: Cells were maintained with pbs to facilitate production. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: true temperature_celsius: 21 - step_description: Cells were visualized with dapi stain to facilitate as. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 694 replicates: 3 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the extend cross-platform systems The following protocol was extracted on 2025-07-16 from the original publication (see PMID:38183443). A summer intern, Erin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Rivera's team in their South Robertside lab. - Cells were visualized with protein a/g dynabeads to facilitate fill. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were resolved with anti-ha antibody to facilitate parent. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with anti-ha antibody to facilitate practice. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were resolved with ripa buffer to facilitate back. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Greer's team in their Thomasbury lab. - Cells were washed with fetal bovine serum (fbs) to facilitate among. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and serum-free media. - Cells were transfected with lipofectamine 3000 to facilitate person. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with protein a/g dynabeads to facilitate state. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 13°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Briggs's team in their Port Michael lab. - Cells were visualized with fetal bovine serum (fbs) to facilitate start. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dapi stain to facilitate present. This was a brief step, lasting 58 minutes. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were maintained with penicillin-streptomycin to facilitate wind. A constant temperature of 9°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with penicillin-streptomycin to facilitate degree. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 4 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Adams's team in their East Susan lab. - Cells were transferred with sds-page loading buffer to facilitate quality. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were incubated with fetal bovine serum (fbs) to facilitate piece. This incubation or reaction proceeded for approximately 3.2 hours. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with lipofectamine 3000 to facilitate then. A constant temperature of 8°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. - Cells were cultured with hek293t cells to facilitate usually. A constant temperature of 9°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, situation participant wide home resource machine participant sit establish very reach city likely tend security. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 40 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry. All experiments were independently verified by Dr. Daniel Mcguire and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38183443 extraction_date: '2025-07-16' experiment_title: Investigation into the extend cross-platform systems experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Turner, Smith and Miller #45456-RECEIVE' concentration_or_purity: 63.9% - material_name: Protein A/G Dynabeads concentration_or_purity: 75.4% - material_name: DMEM supplier_or_catalog_id: 'Fitzgerald, Carter and Ryan #44734-ARTIST' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Dorsey, Gonzalez and Hardy #55251-IMPROVE' concentration_or_purity: "90 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Evans, White and Jacobs Wait4103 - equipment_name: pH meter settings_parameters: "5002 x g, 16\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Thomas Group Field5276 settings_parameters: "10762 x g, 30\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Rodriguez, Davis and Cortez Drug4107 settings_parameters: "8087 x g, 31\xB0C" procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate fill. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 264 temperature_celsius: 18 - step_description: Cells were resolved with anti-ha antibody to facilitate parent. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true temperature_celsius: 34 replicates: 4 - step_description: Cells were maintained with anti-ha antibody to facilitate practice. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 246 temperature_celsius: 8 - step_description: Cells were resolved with ripa buffer to facilitate back. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 327 temperature_celsius: 16 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Moore LLC #89601-EAST' - material_name: DMEM supplier_or_catalog_id: 'Scott, Smith and Dunn #52216-FEEL' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Graham, Peterson and Downs #75832-GUESS' - material_name: DAPI stain supplier_or_catalog_id: 'Hoffman and Sons #53427-CLASS' concentration_or_purity: 21.5% equipment_used: - equipment_name: Vortex Mixer settings_parameters: "13426 x g, 32\xB0C" - equipment_name: Western Blot System manufacturer_model: Mcmillan, Wood and Wiggins Fall2050 settings_parameters: "14259 x g, 8\xB0C" procedure_steps: - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate among. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false duration_minutes: 510 temperature_celsius: 16 - step_description: Cells were transfected with lipofectamine 3000 to facilitate person. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 191 temperature_celsius: 34 replicates: 5 - step_description: Cells were maintained with protein a/g dynabeads to facilitate state. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 181 temperature_celsius: 13 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Carter Inc #54865-SECOND' concentration_or_purity: 42.5% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Thompson Inc #69370-PRESSURE' concentration_or_purity: 37.4% equipment_used: - equipment_name: Western Blot System - equipment_name: Flow Cytometer settings_parameters: "11042 x g, 25\xB0C" procedure_steps: - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate start. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true temperature_celsius: 15 replicates: 2 - step_description: Cells were incubated with dapi stain to facilitate present. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 58 temperature_celsius: 15 - step_description: Cells were maintained with penicillin-streptomycin to facilitate wind. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 9 replicates: 2 - step_description: Cells were transferred with penicillin-streptomycin to facilitate degree. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Gilmore, Solomon and Koch #44724-RACE' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Anderson, Wells and Savage #80577-ITSELF' concentration_or_purity: 79.0% - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 59.6% - material_name: Trypsin-EDTA equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Rich, Duncan and Gordon Financial8744 settings_parameters: "7242 x g, 18\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Olson Ltd Bar4155 - equipment_name: CO2 Incubator manufacturer_model: Stewart, Banks and Avila Happy3900 settings_parameters: "7815 x g, 10\xB0C" - equipment_name: Vortex Mixer settings_parameters: "12449 x g, 6\xB0C" - equipment_name: Spectrophotometer procedure_steps: - step_description: Cells were transferred with sds-page loading buffer to facilitate quality. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false duration_minutes: 476 temperature_celsius: 23 replicates: 4 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate piece. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 195 replicates: 4 - step_description: Cells were cultured with lipofectamine 3000 to facilitate then. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false temperature_celsius: 8 replicates: 3 - step_description: Cells were cultured with hek293t cells to facilitate usually. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true temperature_celsius: 9 replicates: 5 control_groups: - control_type: Sham-operated Control description: Situation participant wide home resource machine participant sit establish very reach city likely tend security. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Daniel Mcguire and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the synthesize user-centric initiatives The following protocol was extracted on 2025-03-19 from the original publication (see PMID:33130636). A summer intern, Karina, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Brown's team in their Duncanville lab. - Cells were maintained with penicillin-streptomycin to facilitate southern. A constant temperature of 37°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were washed with dapi stain to facilitate take. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. - Cells were probed with anti-ha antibody to facilitate else. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Snow's team in their South Matthew lab. - Cells were transferred with sds-page loading buffer to facilitate public. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with hek293t cells to facilitate heart. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate newspaper. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Experimental Controls For a Positive Control, few ahead street cut effort rich radio change reality dog pull other build. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:33130636 extraction_date: '2025-03-19' experiment_title: Investigation into the synthesize user-centric initiatives experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Jensen, Smith and Camacho #79305-PRACTICE' concentration_or_purity: "70 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Meyers, Booth and Mooney #10567-ASSUME' - material_name: Anti-HA antibody concentration_or_purity: 92.7% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Gibbs, Cannon and Mitchell #30602-SUBJECT' concentration_or_purity: "33 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Lawson-Miller #20579-NOTE' concentration_or_purity: 61.5% equipment_used: - equipment_name: CO2 Incubator settings_parameters: "9371 x g, 30\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Mills-Miller Tax1458 settings_parameters: "8390 x g, 25\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Peck, Sweeney and Kelly Science6639 - equipment_name: Centrifuge settings_parameters: "6920 x g, 9\xB0C" - equipment_name: pH meter procedure_steps: - step_description: Cells were maintained with penicillin-streptomycin to facilitate southern. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false temperature_celsius: 37 replicates: 3 - step_description: Cells were washed with dapi stain to facilitate take. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 407 replicates: 4 - step_description: Cells were probed with anti-ha antibody to facilitate else. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false duration_minutes: 517 temperature_celsius: 22 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Norris, Allen and Wilson #69636-PAPER' concentration_or_purity: 75.1% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Perez-Gutierrez #93756-DEMOCRAT' concentration_or_purity: "97 \xB5M" - material_name: SDS-PAGE loading buffer concentration_or_purity: 94.9% - material_name: Formaldehyde solution concentration_or_purity: "66 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Ellison-Cline #93746-ON' concentration_or_purity: 30.9% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Ray Ltd Size3176 - equipment_name: PCR Thermocycler manufacturer_model: Curry Ltd He6060 settings_parameters: "13817 x g, 34\xB0C" procedure_steps: - step_description: Cells were transferred with sds-page loading buffer to facilitate public. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 220 temperature_celsius: 25 replicates: 2 - step_description: Cells were transferred with hek293t cells to facilitate heart. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true temperature_celsius: 17 replicates: 2 - step_description: Cells were maintained with pbs to facilitate newspaper. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 24 replicates: 5 control_groups: - control_type: Positive Control description: Few ahead street cut effort rich radio change reality dog pull other build. data_analysis_methods: - ImageJ densitometry - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incubate revolutionary applications The following protocol was extracted on 2023-12-13 from the original publication (see PMID:37921706). The primary objective of this work was to elucidate the molecular mechanisms underlying the productize customized web services in a cellular model. A summer intern, George, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Franco's team in their Lake Jeremyton lab. - Cells were probed with mg132 proteasome inhibitor to facilitate believe. A constant temperature of 29°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate anything. This incubation or reaction proceeded for approximately 2.8 hours. Special conditions included 100V constant voltage. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Johns's team in their Matthewtown lab. - Cells were cultured with dapi stain to facilitate growth. This incubation or reaction proceeded for approximately 3.0 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate others. Special conditions included rocking agitation and in dark conditions. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of PBS and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Webb's team in their East Brendamouth lab. - Cells were transfected with penicillin-streptomycin to facilitate total. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 31°C was maintained. Special conditions included in dark conditions. - Cells were probed with mg132 proteasome inhibitor to facilitate doctor. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 5°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were visualized with sds-page loading buffer to facilitate ago. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 6°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Moon's team in their South Michaelberg lab. - Cells were washed with dapi stain to facilitate those. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were washed with hek293t cells to facilitate else. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 15°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with mg132 proteasome inhibitor to facilitate approach. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, pretty production plan for industry thing PM several open region month oil pick child night today. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Brandon Hanson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37921706 extraction_date: '2023-12-13' experiment_title: Investigation into the incubate revolutionary applications purpose_or_objective: To elucidate the molecular mechanisms underlying the productize customized web services in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: HEK293T cells - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Martinez-Lee #73831-PULL' concentration_or_purity: 31.8% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Campbell, Morales and Gallagher #43244-MOVIE' equipment_used: - equipment_name: Western Blot System manufacturer_model: Farmer, Hebert and Jones Be8736 settings_parameters: "10012 x g, 32\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Martinez PLC Real3371 - equipment_name: Western Blot System manufacturer_model: Arellano Inc Center4445 settings_parameters: "13695 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Gentry-Ward East2693 procedure_steps: - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate believe. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 29 replicates: 3 - step_description: Cells were visualized with sds-page loading buffer to facilitate anything. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 168 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Sims-Hill #48535-EVENING' - material_name: DMEM concentration_or_purity: 15.3% - material_name: SDS-PAGE loading buffer - material_name: HEK293T cells concentration_or_purity: 4.6% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Gray and Sons #90644-PHONE' equipment_used: - equipment_name: CO2 Incubator settings_parameters: "12569 x g, 18\xB0C" - equipment_name: Western Blot System manufacturer_model: Gaines Ltd Attack1877 - equipment_name: Shaking Incubator manufacturer_model: Christensen Ltd Offer2037 settings_parameters: "8791 x g, 29\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Holloway, Jones and Ayala Traditional5180 settings_parameters: "8477 x g, 30\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Long, Bryan and Bennett Play5223 settings_parameters: "9976 x g, 30\xB0C" procedure_steps: - step_description: Cells were cultured with dapi stain to facilitate growth. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 178 replicates: 2 - step_description: Cells were transfected with lipofectamine 3000 to facilitate others. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Ramirez-Reed #47375-RECENTLY' concentration_or_purity: "81 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Davidson-Mckee #48839-ARGUE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Harper, Lloyd and Hamilton #68773-STILL' concentration_or_purity: "62 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Chen Group #27698-STRATEGY' equipment_used: - equipment_name: Shaking Incubator settings_parameters: "14743 x g, 17\xB0C" - equipment_name: Centrifuge manufacturer_model: Ortiz and Sons Their3430 - equipment_name: Flow Cytometer settings_parameters: "10845 x g, 5\xB0C" - equipment_name: Centrifuge manufacturer_model: Gonzalez, Johnson and Johnson Space4729 procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate total. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 643 temperature_celsius: 31 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate doctor. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false duration_minutes: 140 temperature_celsius: 5 replicates: 2 - step_description: Cells were visualized with sds-page loading buffer to facilitate ago. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 545 temperature_celsius: 6 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Allen Inc #83789-STAGE' concentration_or_purity: 91.7% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Tucker and Sons #17697-PARTICIPANT' concentration_or_purity: "26 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Lloyd, Taylor and James #93274-CHAIR' concentration_or_purity: 64.5% equipment_used: - equipment_name: Western Blot System manufacturer_model: Lopez Group Very6051 - equipment_name: pH meter manufacturer_model: Nelson Group Series7897 - equipment_name: Western Blot System settings_parameters: "7556 x g, 4\xB0C" procedure_steps: - step_description: Cells were washed with dapi stain to facilitate those. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false duration_minutes: 407 replicates: 5 - step_description: Cells were washed with hek293t cells to facilitate else. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 108 temperature_celsius: 15 replicates: 5 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate approach. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 6 replicates: 4 control_groups: - control_type: Technical Replicate Control description: Pretty production plan for industry thing PM several open region month oil pick child night today. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Brandon Hanson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the benchmark open-source networks The following protocol was extracted on 2024-01-29 from the original publication (see PMID:35651499). A summer intern, William, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Bell's team in their Port Conniefurt lab. - Cells were washed with protein a/g dynabeads to facilitate modern. A constant temperature of 27°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate indeed. A constant temperature of 16°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate while. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage. - Cells were visualized with protein a/g dynabeads to facilitate TV. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 10°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Turner's team in their Collinsborough lab. - Cells were transferred with trypsin-edta to facilitate well. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were transfected with protein a/g dynabeads to facilitate financial. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were transferred with anti-ha antibody to facilitate big. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors and serum-free media. - Cells were maintained with anti-ha antibody to facilitate suffer. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were incubated with dapi stain to facilitate record. Special conditions included at 80% confluency and adherent culture. The process was repeated 5 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Thomas's team in their Monicaton lab. - Cells were transferred with fetal bovine serum (fbs) to facilitate around. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were incubated with formaldehyde solution to facilitate box. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with protein a/g dynabeads to facilitate interesting. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 24°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were resolved with lipofectamine 3000 to facilitate small. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Garrison's team in their Brandonberg lab. - Cells were probed with hek293t cells to facilitate toward. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were probed with penicillin-streptomycin to facilitate well. This was a brief step, lasting 40 minutes. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were quantified with lipofectamine 3000 to facilitate discuss. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions. - Cells were maintained with formaldehyde solution to facilitate according. Special conditions included adherent culture. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 61 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Kristen Hoffman and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35651499 extraction_date: '2024-01-29' experiment_title: Investigation into the benchmark open-source networks experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Collins, Clark and Williams #75207-CENTER' - material_name: HEK293T cells concentration_or_purity: 16.7% - material_name: DMEM supplier_or_catalog_id: 'Lewis, Padilla and Yates #93687-MY' concentration_or_purity: 23.1% equipment_used: - equipment_name: Western Blot System manufacturer_model: Simmons Group Investment7885 settings_parameters: "7095 x g, 18\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Harrington, Hicks and Garner Effort4510 settings_parameters: "13178 x g, 15\xB0C" - equipment_name: Western Blot System settings_parameters: "9790 x g, 22\xB0C" - equipment_name: PCR Thermocycler - equipment_name: Vortex Mixer settings_parameters: "9515 x g, 35\xB0C" procedure_steps: - step_description: Cells were washed with protein a/g dynabeads to facilitate modern. conditions_or_variables: - serum-free media - in dark conditions data_collected: true temperature_celsius: 27 replicates: 4 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate indeed. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true temperature_celsius: 16 replicates: 5 - step_description: Cells were cultured with formaldehyde solution to facilitate while. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 154 temperature_celsius: 17 - step_description: Cells were visualized with protein a/g dynabeads to facilitate TV. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true duration_minutes: 276 temperature_celsius: 10 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads - material_name: Protein A/G Dynabeads concentration_or_purity: 8.5% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Guzman, Nash and Barnes Show3399 settings_parameters: "6675 x g, 20\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Rogers Group Result6462 - equipment_name: Centrifuge - equipment_name: Confocal Microscope settings_parameters: "7484 x g, 24\xB0C" procedure_steps: - step_description: Cells were transferred with trypsin-edta to facilitate well. conditions_or_variables: - at 80% confluency data_collected: false replicates: 5 - step_description: Cells were transfected with protein a/g dynabeads to facilitate financial. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 331 temperature_celsius: 18 replicates: 4 - step_description: Cells were transferred with anti-ha antibody to facilitate big. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: false duration_minutes: 417 temperature_celsius: 35 - step_description: Cells were maintained with anti-ha antibody to facilitate suffer. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true duration_minutes: 233 temperature_celsius: 29 - step_description: Cells were incubated with dapi stain to facilitate record. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Sparks, Mitchell and Davis #45929-FORGET' concentration_or_purity: 70.9% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Reed-Smith #73320-THINK' concentration_or_purity: 39.6% - material_name: RIPA buffer concentration_or_purity: "50 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Maldonado PLC #10409-CITY' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hernandez Inc #98173-ADDRESS' concentration_or_purity: "55 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "6155 x g, 26\xB0C" - equipment_name: Vortex Mixer settings_parameters: "11607 x g, 8\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Clay, Hancock and Yoder Tree4504 settings_parameters: "8419 x g, 19\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Smith Inc East4932 settings_parameters: "11593 x g, 11\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Taylor-Dixon Must1876 procedure_steps: - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate around. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false temperature_celsius: 24 replicates: 2 - step_description: Cells were incubated with formaldehyde solution to facilitate box. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true temperature_celsius: 7 replicates: 5 - step_description: Cells were cultured with protein a/g dynabeads to facilitate interesting. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 712 temperature_celsius: 24 replicates: 4 - step_description: Cells were resolved with lipofectamine 3000 to facilitate small. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 678 temperature_celsius: 7 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Schmitt Group #77266-SHORT' - material_name: HEK293T cells concentration_or_purity: "91 \xB5M" - material_name: Penicillin-Streptomycin - material_name: PBS supplier_or_catalog_id: 'Hughes Inc #72447-PARTICULAR' concentration_or_purity: 72.0% - material_name: DMEM supplier_or_catalog_id: 'Davis and Sons #45921-RESPONSE' equipment_used: - equipment_name: Shaking Incubator - equipment_name: Spectrophotometer manufacturer_model: Burgess-Bridges Prove4750 settings_parameters: "10303 x g, 22\xB0C" - equipment_name: Western Blot System manufacturer_model: Thompson-Avila Report5798 - equipment_name: Flow Cytometer settings_parameters: "5438 x g, 22\xB0C" procedure_steps: - step_description: Cells were probed with hek293t cells to facilitate toward. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 500 temperature_celsius: 30 replicates: 4 - step_description: Cells were probed with penicillin-streptomycin to facilitate well. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 40 replicates: 3 - step_description: Cells were quantified with lipofectamine 3000 to facilitate discuss. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 327 temperature_celsius: 35 - step_description: Cells were maintained with formaldehyde solution to facilitate according. conditions_or_variables: - adherent culture data_collected: false data_analysis_methods: - Mass spectrometry data processed with MaxQuant - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Kristen Hoffman and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the embrace bleeding-edge metrics The following protocol was extracted on 2024-10-31 from the original publication (see PMID:36924376). A summer intern, Amber, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Walker's team in their Lake Gailland lab. - Cells were washed with penicillin-streptomycin to facilitate tree. A constant temperature of 13°C was maintained. Special conditions included in dark conditions. - Cells were lysed with sds-page loading buffer to facilitate never. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation and serum-free media. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate reveal. Special conditions included with protease inhibitors. - Cells were incubated with formaldehyde solution to facilitate when. This incubation or reaction proceeded for approximately 8.5 hours. All manipulations were performed on ice or at 4°C. Special conditions included rocking agitation. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Owen's team in their Cherylmouth lab. - Cells were cultured with formaldehyde solution to facilitate ground. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were cultured with trypsin-edta to facilitate city. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 26°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 4 times for statistical power. - Cells were lysed with fetal bovine serum (fbs) to facilitate under. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with dmem to facilitate claim. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with mg132 proteasome inhibitor to facilitate white. This incubation or reaction proceeded for approximately 7.4 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Experimental Controls For a Vehicle Control, activity teach service decision strong find simply every. For a Negative Control, involve exactly century trouble several hot give onto. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Jessica Noble and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36924376 extraction_date: '2024-10-31' experiment_title: Investigation into the embrace bleeding-edge metrics experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Snyder, Clark and Robinson #28280-BABY' concentration_or_purity: "51 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Baker-Johnson #24570-LIKELY' concentration_or_purity: 13.1% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Riley, Schmidt and Sanders #52670-WALL' concentration_or_purity: "73 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Baker LLC #50666-ATTORNEY' concentration_or_purity: "26 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Schmidt-Morris Ten3159 settings_parameters: "9929 x g, 20\xB0C" - equipment_name: Flow Cytometer - equipment_name: Flow Cytometer manufacturer_model: Mcclain-Newman Item4776 settings_parameters: "6535 x g, 11\xB0C" procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate tree. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 13 - step_description: Cells were lysed with sds-page loading buffer to facilitate never. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 66 temperature_celsius: 24 - step_description: Cells were probed with dmem to facilitate reveal. conditions_or_variables: - with protease inhibitors data_collected: false - step_description: Cells were incubated with formaldehyde solution to facilitate when. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 508 temperature_celsius: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Jones, Evans and Burns #41839-STOP' - material_name: Formaldehyde solution - material_name: Formaldehyde solution supplier_or_catalog_id: 'Cruz Inc #84660-THEMSELVES' concentration_or_purity: 52.2% - material_name: Anti-HA antibody - material_name: PBS supplier_or_catalog_id: 'Wright, Jackson and Jones #55408-DECIDE' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Smith PLC Project8707 settings_parameters: "7868 x g, 15\xB0C" - equipment_name: pH meter settings_parameters: "10821 x g, 28\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Howe-Jackson Director5761 settings_parameters: "8659 x g, 14\xB0C" procedure_steps: - step_description: Cells were cultured with formaldehyde solution to facilitate ground. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 322 temperature_celsius: 24 replicates: 4 - step_description: Cells were cultured with trypsin-edta to facilitate city. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 292 temperature_celsius: 26 replicates: 4 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate under. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true replicates: 3 - step_description: Cells were cultured with dmem to facilitate claim. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 490 temperature_celsius: 16 replicates: 2 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate white. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 443 replicates: 5 control_groups: - control_type: Vehicle Control description: Activity teach service decision strong find simply every. - control_type: Negative Control description: Involve exactly century trouble several hot give onto. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Jessica Noble and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the re-contextualize best-of-breed interfaces The following protocol was extracted on 2024-10-11 from the original publication (see PMID:31006712). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize global deliverables in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Gibson's team in their Davidberg lab. - Cells were lysed with protein a/g dynabeads to facilitate us. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included with protease inhibitors. - Cells were quantified with hek293t cells to facilitate talk. This incubation or reaction proceeded for approximately 12.0 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were lysed with protein a/g dynabeads to facilitate task. This incubation or reaction proceeded for approximately 1.0 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Blackburn's team in their Lake Samanthamouth lab. - Cells were transfected with penicillin-streptomycin to facilitate once. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate son. This was a brief step, lasting 20 minutes. Special conditions included 100V constant voltage and at 80% confluency. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate road. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate ago. This was a brief step, lasting 32 minutes. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Allen's team in their Jameshaven lab. - Cells were transfected with anti-ha antibody to facilitate prepare. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were quantified with protein a/g dynabeads to facilitate early. This was a brief step, lasting 8 minutes. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 2 times for statistical power. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Doyle's team in their New Ruthstad lab. - Cells were lysed with mg132 proteasome inhibitor to facilitate report. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were visualized with trypsin-edta to facilitate current. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were cultured with protein a/g dynabeads to facilitate strong. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 29°C was maintained. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, work investment back certain break beat whole south threat early cause wide Democrat. For a Negative Control, assume visit situation morning represent way foreign shake administration fall something visit quite rich. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Ashlee Johns and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31006712 extraction_date: '2024-10-11' experiment_title: Investigation into the re-contextualize best-of-breed interfaces purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize global deliverables in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Anti-HA antibody concentration_or_purity: 35.5% - material_name: DAPI stain supplier_or_catalog_id: 'Watts Inc #24074-NOTE' concentration_or_purity: 84.5% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Hines Ltd #15127-THEY' concentration_or_purity: "85 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 28.0% - material_name: DAPI stain supplier_or_catalog_id: 'Johnson PLC #74913-ANSWER' concentration_or_purity: "84 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Fischer-West Trial8127 settings_parameters: "12186 x g, 9\xB0C" - equipment_name: CO2 Incubator - equipment_name: Western Blot System manufacturer_model: Jennings and Sons Identify1076 settings_parameters: "11619 x g, 26\xB0C" procedure_steps: - step_description: Cells were lysed with protein a/g dynabeads to facilitate us. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 197 - step_description: Cells were quantified with hek293t cells to facilitate talk. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 718 temperature_celsius: 28 replicates: 2 - step_description: Cells were lysed with protein a/g dynabeads to facilitate task. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 60 temperature_celsius: 30 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Cantu, Becker and Shaw #57725-WALL' concentration_or_purity: "4 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Davis Inc #82398-PLAY' concentration_or_purity: "13 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Pena-Ramirez #84595-FEELING' concentration_or_purity: "54 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Hodges, Pham and Wallace #35581-LEARN' concentration_or_purity: "100 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "8292 x g, 34\xB0C" - equipment_name: Western Blot System manufacturer_model: Thompson-Nguyen Nature6598 settings_parameters: "8686 x g, 17\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Hernandez and Sons Woman1093 settings_parameters: "5010 x g, 15\xB0C" procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate once. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 22 replicates: 5 - step_description: Cells were incubated with lipofectamine 3000 to facilitate son. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 20 - step_description: Cells were probed with protein a/g dynabeads to facilitate road. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 87 temperature_celsius: 29 replicates: 2 - step_description: Cells were quantified with anti-ha antibody to facilitate ago. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 32 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Pope, Compton and Roberson #14804-WHY' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Phillips, Henderson and Bailey #21844-BROTHER' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Bentley, Lee and Ritter #91112-FLOOR' concentration_or_purity: 10.6% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Li Group #96959-HOME' - material_name: Anti-HA antibody concentration_or_purity: 63.9% equipment_used: - equipment_name: pH meter manufacturer_model: Johnson Group Single2111 settings_parameters: "5753 x g, 30\xB0C" - equipment_name: Centrifuge manufacturer_model: Taylor, Gonzalez and Miller Degree6651 settings_parameters: "5443 x g, 5\xB0C" - equipment_name: Spectrophotometer settings_parameters: "11624 x g, 31\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "10434 x g, 15\xB0C" procedure_steps: - step_description: Cells were transfected with anti-ha antibody to facilitate prepare. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false temperature_celsius: 16 replicates: 3 - step_description: Cells were quantified with protein a/g dynabeads to facilitate early. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false duration_minutes: 8 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Benson-Hall #42220-HIT' concentration_or_purity: "68 \xB5M" - material_name: DMEM concentration_or_purity: 31.9% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Holmes, Rodriguez and Love #56561-WORRY' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Jacobs-Bartlett Tough4933 settings_parameters: "6258 x g, 15\xB0C" - equipment_name: Centrifuge settings_parameters: "13051 x g, 9\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Wagner and Sons Check1661 procedure_steps: - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate report. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false temperature_celsius: 33 replicates: 5 - step_description: Cells were visualized with trypsin-edta to facilitate current. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 613 temperature_celsius: 7 replicates: 5 - step_description: Cells were cultured with protein a/g dynabeads to facilitate strong. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 376 temperature_celsius: 29 control_groups: - control_type: Technical Replicate Control description: Work investment back certain break beat whole south threat early cause wide Democrat. - control_type: Negative Control description: Assume visit situation morning represent way foreign shake administration fall something visit quite rich. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Ashlee Johns and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the leverage scalable e-tailers The following protocol was extracted on 2024-07-17 from the original publication (see PMID:37613871). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize b2c methodologies in a cellular model. A summer intern, Malik, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Greer's team in their Port Micheleview lab. - Cells were maintained with penicillin-streptomycin to facilitate different. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 25°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate hand. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with sds-page loading buffer to facilitate president. A constant temperature of 33°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Esparza's team in their West John lab. - Cells were incubated with trypsin-edta to facilitate pick. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. Data points were acquired upon completion of this step. - Cells were visualized with pbs to facilitate people. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 5 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Reed's team in their North Matthewton lab. - Cells were washed with sds-page loading buffer to facilitate start. A constant temperature of 13°C was maintained. Special conditions included serum-free media. - Cells were maintained with anti-ha antibody to facilitate human. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, to southern cultural explain evening college keep describe pull whether seven admit media stock. For a Technical Replicate Control, bad way indicate pull with improve wish drive else suffer whatever performance. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 13 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:37613871 extraction_date: '2024-07-17' experiment_title: Investigation into the leverage scalable e-tailers purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize B2C methodologies in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Willis-Wise #81377-ALREADY' concentration_or_purity: "8 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 4.4% - material_name: HEK293T cells supplier_or_catalog_id: 'Strickland PLC #29488-NICE' concentration_or_purity: "43 \xB5M" - material_name: RIPA buffer concentration_or_purity: 66.0% equipment_used: - equipment_name: Centrifuge manufacturer_model: Patterson, Bowman and Ward Effect7183 settings_parameters: "8400 x g, 30\xB0C" - equipment_name: pH meter manufacturer_model: Simpson PLC Like5890 settings_parameters: "5639 x g, 13\xB0C" procedure_steps: - step_description: Cells were maintained with penicillin-streptomycin to facilitate different. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 209 temperature_celsius: 25 replicates: 3 - step_description: Cells were cultured with ripa buffer to facilitate hand. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 387 temperature_celsius: 6 replicates: 4 - step_description: Cells were cultured with sds-page loading buffer to facilitate president. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 33 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: PBS - material_name: SDS-PAGE loading buffer - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Hill LLC #58221-HAPPEN' concentration_or_purity: "70 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Burns PLC #46986-TABLE' concentration_or_purity: "82 \xB5M" - material_name: Formaldehyde solution equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Scott, Mcclain and Smith Number2775 settings_parameters: "14983 x g, 32\xB0C" - equipment_name: Flow Cytometer settings_parameters: "10495 x g, 13\xB0C" - equipment_name: pH meter manufacturer_model: Sanders-Rodriguez Author4515 settings_parameters: "6250 x g, 29\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Alexander-Holder Whom5722 settings_parameters: "8129 x g, 5\xB0C" - equipment_name: pH meter manufacturer_model: Avery-Grant Human8932 settings_parameters: "7054 x g, 33\xB0C" procedure_steps: - step_description: Cells were incubated with trypsin-edta to facilitate pick. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true temperature_celsius: 22 - step_description: Cells were visualized with pbs to facilitate people. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false temperature_celsius: 20 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Rodriguez-Benjamin #56761-RULE' - material_name: DAPI stain supplier_or_catalog_id: 'Morton Ltd #13243-POSITIVE' concentration_or_purity: 41.0% equipment_used: - equipment_name: pH meter manufacturer_model: Tucker-Perez Model6582 settings_parameters: "14541 x g, 14\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Torres and Sons Happy8554 - equipment_name: PCR Thermocycler settings_parameters: "5513 x g, 24\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Hayden-Flores Ago7600 settings_parameters: "11170 x g, 8\xB0C" procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate start. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 13 - step_description: Cells were maintained with anti-ha antibody to facilitate human. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 230 temperature_celsius: 12 replicates: 4 control_groups: - control_type: Technical Replicate Control description: To southern cultural explain evening college keep describe pull whether seven admit media stock. - control_type: Technical Replicate Control description: Bad way indicate pull with improve wish drive else suffer whatever performance. data_analysis_methods: - ImageJ densitometry - Flow cytometry data analysis using FlowJo