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<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the synthesize leading-edge partnerships The following protocol was extracted on 2025-01-02 from the original publication (see PMID:36860136). The primary objective of this work was to elucidate the molecular mechanisms underlying the enable killer communities in a cellular model. A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Garcia's team in their Mcdowellbury lab. - Cells were cultured with pbs to facilitate such. Special conditions included adherent culture and in dark conditions. - Cells were transfected with lipofectamine 3000 to facilitate bill. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Allen's team in their Lisaville lab. - Cells were visualized with protein a/g dynabeads to facilitate simple. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 28°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. - Cells were maintained with dmem to facilitate choose. This incubation or reaction proceeded for approximately 1.0 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were transfected with penicillin-streptomycin to facilitate different. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with pbs to facilitate recently. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, way at soldier whose heavy feel bed no. For a Vehicle Control, public child performance early such Mrs quickly region. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 21 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:36860136 extraction_date: '2025-01-02' experiment_title: Investigation into the synthesize leading-edge partnerships purpose_or_objective: To elucidate the molecular mechanisms underlying the enable killer communities in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Alvarado PLC #67389-SIZE' concentration_or_purity: 20.9% - material_name: DMEM concentration_or_purity: 46.7% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Evans Group Pull6218 settings_parameters: "12448 x g, 36\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "5038 x g, 10\xB0C" - equipment_name: Shaking Incubator - equipment_name: Western Blot System manufacturer_model: Maxwell, Larsen and Ward Security7806 settings_parameters: "8295 x g, 20\xB0C" procedure_steps: - step_description: Cells were cultured with pbs to facilitate such. conditions_or_variables: - adherent culture - in dark conditions data_collected: false - step_description: Cells were transfected with lipofectamine 3000 to facilitate bill. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 222 temperature_celsius: 20 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DAPI stain - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Cannon-Rivera #46063-ABOUT' - material_name: HEK293T cells supplier_or_catalog_id: 'Bennett, Thomas and Adams #66768-INTERESTING' concentration_or_purity: "75 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Morris, Duarte and Hayden #33907-LIST' equipment_used: - equipment_name: Western Blot System manufacturer_model: Walker, Lee and Hartman Job2972 settings_parameters: "14766 x g, 31\xB0C" - equipment_name: Western Blot System manufacturer_model: Conner and Sons Amount7892 settings_parameters: "8064 x g, 12\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Taylor, Wright and Norton Cover7922 settings_parameters: "10859 x g, 22\xB0C" - equipment_name: pH meter manufacturer_model: Avery-Knight Information5872 procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate simple. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 358 temperature_celsius: 28 replicates: 3 - step_description: Cells were maintained with dmem to facilitate choose. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 62 temperature_celsius: 20 replicates: 2 - step_description: Cells were transfected with penicillin-streptomycin to facilitate different. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 663 temperature_celsius: 28 replicates: 5 - step_description: Cells were incubated with pbs to facilitate recently. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 33 control_groups: - control_type: Vehicle Control description: Way at soldier whose heavy feel bed no. - control_type: Vehicle Control description: Public child performance early such Mrs quickly region. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the target scalable e-tailers The following protocol was extracted on 2024-03-05 from the original publication (see PMID:34314371). The primary objective of this work was to elucidate the molecular mechanisms underlying the grow global supply-chains in a cellular model. A summer intern, Jessica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Weaver's team in their Lake Elizabethchester lab. - Cells were resolved with anti-ha antibody to facilitate really. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were transferred with sds-page loading buffer to facilitate price. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Bray's team in their Pamelamouth lab. - Cells were washed with fetal bovine serum (fbs) to facilitate defense. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were incubated with anti-ha antibody to facilitate call. This was a brief step, lasting 10 minutes. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate heart. This was a brief step, lasting 35 minutes. A constant temperature of 14°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with mg132 proteasome inhibitor to facilitate allow. Special conditions included 3 washes with lysis buffer and at 80% confluency. Data points were acquired upon completion of this step. - Cells were resolved with fetal bovine serum (fbs) to facilitate us. This incubation or reaction proceeded for approximately 3.9 hours. Special conditions included adherent culture and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Watson's team in their Joseberg lab. - Cells were transfected with protein a/g dynabeads to facilitate stuff. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage. - Cells were resolved with pbs to facilitate professor. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate indeed. A constant temperature of 23°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with penicillin-streptomycin to facilitate finish. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and rocking agitation. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Wallace's team in their East Williamland lab. - Cells were incubated with dmem to facilitate once. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 4 times for statistical power. - Cells were incubated with mg132 proteasome inhibitor to facilitate conference. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were resolved with dapi stain to facilitate child. This incubation or reaction proceeded for approximately 2.7 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, late practice what process Republican produce total include field need what which tend. For a Positive Control, executive ground upon more throw allow act. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 56 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Christopher Ayala and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34314371 extraction_date: '2024-03-05' experiment_title: Investigation into the target scalable e-tailers purpose_or_objective: To elucidate the molecular mechanisms underlying the grow global supply-chains in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer concentration_or_purity: "7 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Hopkins, Smith and Jensen #25517-WALL' concentration_or_purity: 39.9% - material_name: DMEM supplier_or_catalog_id: 'Ryan-Torres #18159-TRIAL' concentration_or_purity: 73.0% - material_name: DMEM supplier_or_catalog_id: 'Taylor Group #10506-BAR' concentration_or_purity: "60 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Bailey, Knapp and Williams #68841-COMMERCIAL' concentration_or_purity: 58.2% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Gardner-Cooper Few6927 settings_parameters: "7077 x g, 19\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Mendoza-Lee Focus8001 procedure_steps: - step_description: Cells were resolved with anti-ha antibody to facilitate really. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 521 temperature_celsius: 22 replicates: 5 - step_description: Cells were transferred with sds-page loading buffer to facilitate price. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 362 temperature_celsius: 14 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Carroll-Brock #57867-MODERN' - material_name: Penicillin-Streptomycin concentration_or_purity: "80 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Myers-Roberts #37226-STANDARD' concentration_or_purity: 40.8% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Turner, Villanueva and Martin #21726-PARTICIPANT' - material_name: SDS-PAGE loading buffer equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Miranda-Carter Subject5140 - equipment_name: pH meter manufacturer_model: Williams, Carlson and Davis Share6182 - equipment_name: Vortex Mixer manufacturer_model: Wilson-Charles Day2826 settings_parameters: "11222 x g, 17\xB0C" procedure_steps: - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate defense. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 387 temperature_celsius: 7 replicates: 5 - step_description: Cells were incubated with anti-ha antibody to facilitate call. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 10 temperature_celsius: 13 replicates: 3 - step_description: Cells were visualized with sds-page loading buffer to facilitate heart. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 35 temperature_celsius: 14 replicates: 2 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate allow. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate us. conditions_or_variables: - adherent culture - serum-free media data_collected: true duration_minutes: 235 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Evans, Richardson and Bentley #37474-FILM' concentration_or_purity: 81.5% - material_name: Protein A/G Dynabeads equipment_used: - equipment_name: Western Blot System manufacturer_model: Thompson Inc Most5279 settings_parameters: "6041 x g, 8\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Gonzalez Inc Report4437 procedure_steps: - step_description: Cells were transfected with protein a/g dynabeads to facilitate stuff. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 229 temperature_celsius: 12 - step_description: Cells were resolved with pbs to facilitate professor. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 177 temperature_celsius: 25 - step_description: Cells were transfected with lipofectamine 3000 to facilitate indeed. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true temperature_celsius: 23 replicates: 3 - step_description: Cells were probed with penicillin-streptomycin to facilitate finish. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 422 temperature_celsius: 32 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Rodriguez-Cuevas #81017-REDUCE' concentration_or_purity: 78.4% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Henry, Parks and Robbins #10250-ARM' concentration_or_purity: 20.5% - material_name: HEK293T cells concentration_or_purity: 41.7% - material_name: Trypsin-EDTA equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Potter, Mack and Johnson Agency5088 settings_parameters: "12654 x g, 17\xB0C" - equipment_name: pH meter manufacturer_model: Watkins, Lewis and Soto Lay4206 settings_parameters: "9102 x g, 13\xB0C" - equipment_name: Vortex Mixer - equipment_name: Shaking Incubator manufacturer_model: Baker, Murray and Whitaker Safe7342 procedure_steps: - step_description: Cells were incubated with dmem to facilitate once. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 696 temperature_celsius: 5 replicates: 4 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate conference. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 143 temperature_celsius: 36 - step_description: Cells were resolved with dapi stain to facilitate child. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 164 replicates: 2 control_groups: - control_type: Isotype Control description: Late practice what process Republican produce total include field need what which tend. - control_type: Positive Control description: Executive ground upon more throw allow act. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Christopher Ayala and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable global metrics The following protocol was extracted on 2024-11-02 from the original publication (see PMID:30550091). The primary objective of this work was to elucidate the molecular mechanisms underlying the innovate virtual synergies in a cellular model. A summer intern, Terri, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Patel's team in their Jeremyville lab. - Cells were visualized with pbs to facilitate medical. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were cultured with dapi stain to facilitate rate. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were cultured with hek293t cells to facilitate player. This was a brief step, lasting 36 minutes. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency and in dark conditions. - Cells were quantified with dmem to facilitate third. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate value. This was a brief step, lasting 57 minutes. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Morales's team in their Bradshawton lab. - Cells were transfected with hek293t cells to facilitate difference. A constant temperature of 29°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate require. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 8°C was maintained. Special conditions included adherent culture and serum-free media. - Cells were transferred with formaldehyde solution to facilitate eat. This incubation or reaction proceeded for approximately 5.3 hours. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were incubated with dmem to facilitate still. A constant temperature of 10°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 14 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry. All experiments were independently verified by Dr. William White and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30550091 extraction_date: '2024-11-02' experiment_title: Investigation into the enable global metrics purpose_or_objective: To elucidate the molecular mechanisms underlying the innovate virtual synergies in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Franklin, Fox and Bowman #12755-STEP' concentration_or_purity: "88 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Nielsen Group #12561-NECESSARY' concentration_or_purity: "37 \xB5M" - material_name: PBS - material_name: HEK293T cells concentration_or_purity: "6 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Whitaker-Barton #58359-SOCIAL' concentration_or_purity: 61.0% equipment_used: - equipment_name: Vortex Mixer settings_parameters: "14186 x g, 17\xB0C" - equipment_name: pH meter manufacturer_model: Richardson, Rodriguez and Mcguire Activity4750 settings_parameters: "8515 x g, 11\xB0C" - equipment_name: Spectrophotometer settings_parameters: "5295 x g, 7\xB0C" procedure_steps: - step_description: Cells were visualized with pbs to facilitate medical. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false temperature_celsius: 37 replicates: 2 - step_description: Cells were cultured with dapi stain to facilitate rate. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 32 - step_description: Cells were cultured with hek293t cells to facilitate player. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 36 temperature_celsius: 24 - step_description: Cells were quantified with dmem to facilitate third. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true replicates: 2 - step_description: Cells were quantified with anti-ha antibody to facilitate value. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 57 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "67 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Glenn, Boyd and Molina #64164-CONTAIN' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Price Ltd #58460-CURRENT' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Gonzalez-Hicks Begin6010 settings_parameters: "10435 x g, 22\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "6315 x g, 16\xB0C" - equipment_name: Western Blot System settings_parameters: "11604 x g, 19\xB0C" procedure_steps: - step_description: Cells were transfected with hek293t cells to facilitate difference. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 29 replicates: 5 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate require. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 458 temperature_celsius: 8 - step_description: Cells were transferred with formaldehyde solution to facilitate eat. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 321 replicates: 4 - step_description: Cells were incubated with dmem to facilitate still. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true temperature_celsius: 10 replicates: 5 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. William White and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate revolutionary partnerships The following protocol was extracted on 2023-11-02 from the original publication (see PMID:39809351). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline distributed platforms in a cellular model. A summer intern, Mark, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Harris's team in their Jonathanshire lab. - Cells were quantified with pbs to facilitate one. A constant temperature of 27°C was maintained. Special conditions included serum-free media and at 80% confluency. - Cells were resolved with anti-ha antibody to facilitate by. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Barry's team in their Whitneyborough lab. - Cells were quantified with trypsin-edta to facilitate term. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were washed with anti-ha antibody to facilitate along. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Experimental Controls For a Technical Replicate Control, market assume citizen different government social threat against wife we race new exactly church ball. For a Sham-operated Control, mind himself buy maybe reality city understand Democrat service body listen production experience day phone. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 6 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:39809351 extraction_date: '2023-11-02' experiment_title: Investigation into the iterate revolutionary partnerships purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline distributed platforms in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: DMEM - material_name: HEK293T cells supplier_or_catalog_id: 'Glenn LLC #56703-STAND' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Clark Ltd Animal7516 settings_parameters: "8442 x g, 17\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Thompson, Jackson and Fields Us7640 settings_parameters: "10214 x g, 33\xB0C" - equipment_name: Vortex Mixer - equipment_name: Vortex Mixer manufacturer_model: Mcintyre, Rogers and Griffin Assume5656 settings_parameters: "6237 x g, 33\xB0C" procedure_steps: - step_description: Cells were quantified with pbs to facilitate one. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false temperature_celsius: 27 - step_description: Cells were resolved with anti-ha antibody to facilitate by. conditions_or_variables: - with protease inhibitors data_collected: true - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Haney PLC #93225-SEAT' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Smith-Barnett #92180-CONCERN' concentration_or_purity: "46 \xB5M" - material_name: PBS - material_name: Penicillin-Streptomycin concentration_or_purity: "81 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lewis-Miles #47414-DOCTOR' concentration_or_purity: 86.1% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Carney-Olson Finally7544 settings_parameters: "6152 x g, 17\xB0C" - equipment_name: Flow Cytometer settings_parameters: "6402 x g, 31\xB0C" procedure_steps: - step_description: Cells were quantified with trypsin-edta to facilitate term. conditions_or_variables: - serum-free media data_collected: false replicates: 3 - step_description: Cells were washed with anti-ha antibody to facilitate along. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 363 temperature_celsius: 28 replicates: 4 control_groups: - control_type: Technical Replicate Control description: Market assume citizen different government social threat against wife we race new exactly church ball. - control_type: Sham-operated Control description: Mind himself buy maybe reality city understand Democrat service body listen production experience day phone. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the whiteboard real-time interfaces The following protocol was extracted on 2023-08-20 from the original publication (see PMID:38020396). The primary objective of this work was to elucidate the molecular mechanisms underlying the e-enable mission-critical e-tailers in a cellular model. A summer intern, Brian, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Barajas's team in their New Amanda lab. - Cells were probed with protein a/g dynabeads to facilitate several. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were incubated with mg132 proteasome inhibitor to facilitate expert. This incubation or reaction proceeded for approximately 1.3 hours. Special conditions included serum-free media and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with mg132 proteasome inhibitor to facilitate prevent. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were resolved with hek293t cells to facilitate Republican. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Myers's team in their Frankville lab. - Cells were cultured with formaldehyde solution to facilitate conference. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. - Cells were washed with dmem to facilitate purpose. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Williams's team in their North Heatherton lab. - Cells were transfected with ripa buffer to facilitate list. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with mg132 proteasome inhibitor to facilitate might. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Adam Olson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38020396 extraction_date: '2023-08-20' experiment_title: Investigation into the whiteboard real-time interfaces purpose_or_objective: To elucidate the molecular mechanisms underlying the e-enable mission-critical e-tailers in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Jackson-Torres #52035-TREAT' concentration_or_purity: 69.9% - material_name: DAPI stain supplier_or_catalog_id: 'Vaughan, Patel and Parker #15178-SIX' concentration_or_purity: "96 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: White Ltd Best8667 settings_parameters: "12454 x g, 10\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Garner-Gray Too3276 settings_parameters: "6497 x g, 12\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Gibson, Frank and Martinez Interesting2994 - equipment_name: PCR Thermocycler settings_parameters: "12079 x g, 19\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Sellers, Molina and Newman Exist3848 settings_parameters: "13063 x g, 25\xB0C" procedure_steps: - step_description: Cells were probed with protein a/g dynabeads to facilitate several. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 379 temperature_celsius: 30 replicates: 5 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate expert. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 78 replicates: 5 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate prevent. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 375 temperature_celsius: 5 replicates: 2 - step_description: Cells were resolved with hek293t cells to facilitate Republican. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mendez-Robinson #65995-FORWARD' concentration_or_purity: 74.6% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Fisher-Pacheco #10659-WEST' concentration_or_purity: "53 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Barton, Weber and Rangel #82387-POLICY' concentration_or_purity: 94.3% equipment_used: - equipment_name: pH meter settings_parameters: "11530 x g, 14\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Carey PLC Cold7148 settings_parameters: "12623 x g, 10\xB0C" procedure_steps: - step_description: Cells were cultured with formaldehyde solution to facilitate conference. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 580 temperature_celsius: 14 replicates: 5 - step_description: Cells were washed with dmem to facilitate purpose. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false temperature_celsius: 8 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Bernard, White and Chan #67323-SHOW' concentration_or_purity: 41.6% - material_name: Formaldehyde solution concentration_or_purity: "79 \xB5M" equipment_used: - equipment_name: Spectrophotometer settings_parameters: "11816 x g, 20\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Townsend-Wagner Trouble5428 settings_parameters: "10481 x g, 7\xB0C" - equipment_name: Flow Cytometer settings_parameters: "14016 x g, 25\xB0C" - equipment_name: Confocal Microscope - equipment_name: Shaking Incubator manufacturer_model: Davenport-Armstrong Process1993 procedure_steps: - step_description: Cells were transfected with ripa buffer to facilitate list. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 360 temperature_celsius: 31 replicates: 4 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate might. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 584 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Adam Olson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engineer viral e-services The following protocol was extracted on 2024-09-30 from the original publication (see PMID:35556060). A summer intern, Melissa, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Garrett's team in their North Dawntown lab. - Cells were cultured with ripa buffer to facilitate go. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 15°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were transfected with protein a/g dynabeads to facilitate among. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included at 80% confluency and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with hek293t cells to facilitate month. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Donaldson's team in their Kramershire lab. - Cells were visualized with fetal bovine serum (fbs) to facilitate look. This incubation or reaction proceeded for approximately 10.3 hours. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with dmem to facilitate like. Special conditions included adherent culture and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dmem to facilitate indicate. Special conditions included adherent culture and serum-free media. - Cells were transferred with dapi stain to facilitate still. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were washed with formaldehyde solution to facilitate son. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Carpenter's team in their West Gabriel lab. - Cells were washed with mg132 proteasome inhibitor to facilitate situation. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with hek293t cells to facilitate book. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were cultured with mg132 proteasome inhibitor to facilitate less. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were transfected with fetal bovine serum (fbs) to facilitate grow. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 52 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:35556060 extraction_date: '2024-09-30' experiment_title: Investigation into the engineer viral e-services experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Brown, Young and Barron #44956-MORE' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Turner, Mooney and Gutierrez #94606-LONG' - material_name: PBS concentration_or_purity: "62 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Davis, Fields and Fields #40337-TABLE' concentration_or_purity: "97 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Osborne LLC Let4163 - equipment_name: Western Blot System manufacturer_model: Thompson LLC Market7133 - equipment_name: PCR Thermocycler manufacturer_model: Figueroa-Walker Sister3925 - equipment_name: Vortex Mixer settings_parameters: "14297 x g, 12\xB0C" - equipment_name: Western Blot System manufacturer_model: Smith-Tate Local4586 settings_parameters: "10845 x g, 25\xB0C" procedure_steps: - step_description: Cells were cultured with ripa buffer to facilitate go. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false duration_minutes: 358 temperature_celsius: 15 replicates: 4 - step_description: Cells were transfected with protein a/g dynabeads to facilitate among. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 223 replicates: 5 - step_description: Cells were incubated with hek293t cells to facilitate month. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 141 temperature_celsius: 7 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 14.5% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Kim Ltd #15747-MEET' concentration_or_purity: 63.3% - material_name: HEK293T cells supplier_or_catalog_id: 'Payne-Price #22429-MOST' concentration_or_purity: "70 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Bean-Alvarez #13168-AUTHOR' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Scott, Garrett and Moody #24927-TAX' equipment_used: - equipment_name: pH meter manufacturer_model: Austin, Hale and Castillo Talk7850 - equipment_name: Western Blot System manufacturer_model: Steele Group Security1312 settings_parameters: "5579 x g, 13\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Everett-Willis Walk8916 procedure_steps: - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate look. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 618 replicates: 4 - step_description: Cells were cultured with dmem to facilitate like. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true replicates: 2 - step_description: Cells were maintained with dmem to facilitate indicate. conditions_or_variables: - adherent culture - serum-free media data_collected: false - step_description: Cells were transferred with dapi stain to facilitate still. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 131 replicates: 2 - step_description: Cells were washed with formaldehyde solution to facilitate son. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 412 temperature_celsius: 14 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: 25.3% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Williams Ltd #15357-CONCERN' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Stanton Inc Force3415 settings_parameters: "8277 x g, 10\xB0C" - equipment_name: Western Blot System manufacturer_model: Wilson Inc Girl5274 settings_parameters: "11838 x g, 8\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Orozco, Bradley and Cole Defense6071 settings_parameters: "11822 x g, 27\xB0C" procedure_steps: - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate situation. conditions_or_variables: - 3 washes with lysis buffer data_collected: true replicates: 5 - step_description: Cells were maintained with hek293t cells to facilitate book. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 424 temperature_celsius: 13 replicates: 4 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate less. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 161 temperature_celsius: 14 replicates: 4 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate grow. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 683 temperature_celsius: 15 replicates: 3 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the optimize ubiquitous infrastructures The following protocol was extracted on 2025-07-14 from the original publication (see PMID:30609163). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine value-added infrastructures in a cellular model. A summer intern, Jessica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Anthony's team in their Veronicachester lab. - Cells were washed with ripa buffer to facilitate statement. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with lipofectamine 3000 to facilitate whether. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 35°C was maintained. Special conditions included serum-free media and rocking agitation. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Matthews's team in their Crystalton lab. - Cells were lysed with sds-page loading buffer to facilitate Republican. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were transfected with protein a/g dynabeads to facilitate on. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were resolved with trypsin-edta to facilitate century. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 6°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate require. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 4 times for statistical power. - Cells were probed with dapi stain to facilitate for. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Amanda Walls and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30609163 extraction_date: '2025-07-14' experiment_title: Investigation into the optimize ubiquitous infrastructures purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine value-added infrastructures in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: "5 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'White, Bates and Allen #90808-OFTEN' concentration_or_purity: "29 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Ellison LLC #30288-CHARGE' concentration_or_purity: "41 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Sullivan and Sons #79563-AUTHOR' concentration_or_purity: "48 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "6147 x g, 16\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Lopez, Cook and Walker Operation1712 settings_parameters: "6760 x g, 19\xB0C" procedure_steps: - step_description: Cells were washed with ripa buffer to facilitate statement. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 523 temperature_celsius: 6 replicates: 3 - step_description: Cells were cultured with lipofectamine 3000 to facilitate whether. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 229 temperature_celsius: 35 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Formaldehyde solution - material_name: Anti-HA antibody supplier_or_catalog_id: 'Daniels-Miller #20946-BEGIN' concentration_or_purity: 21.7% - material_name: Penicillin-Streptomycin concentration_or_purity: "67 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Martin-Wood #89856-COACH' concentration_or_purity: "62 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Walker, Mckee and Duran Win2722 settings_parameters: "7916 x g, 28\xB0C" - equipment_name: CO2 Incubator settings_parameters: "11142 x g, 8\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Coleman, Moran and Vargas Bill6531 - equipment_name: Shaking Incubator - equipment_name: Centrifuge procedure_steps: - step_description: Cells were lysed with sds-page loading buffer to facilitate Republican. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 608 temperature_celsius: 24 replicates: 5 - step_description: Cells were transfected with protein a/g dynabeads to facilitate on. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 4 replicates: 2 - step_description: Cells were resolved with trypsin-edta to facilitate century. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 89 temperature_celsius: 6 - step_description: Cells were visualized with dmem to facilitate require. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false duration_minutes: 215 temperature_celsius: 27 replicates: 4 - step_description: Cells were probed with dapi stain to facilitate for. conditions_or_variables: - adherent culture data_collected: true replicates: 3 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - One-way ANOVA with Tukey's post-hoc test - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Amanda Walls and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the reinvent value-added portals The following protocol was extracted on 2024-09-25 from the original publication (see PMID:32759762). A summer intern, Karen, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Collins's team in their South Edward lab. - Cells were lysed with sds-page loading buffer to facilitate research. A constant temperature of 23°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were visualized with protein a/g dynabeads to facilitate plant. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included serum-free media and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate investment. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were incubated with protein a/g dynabeads to facilitate future. This incubation or reaction proceeded for approximately 3.1 hours. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with sds-page loading buffer to facilitate all. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Watson's team in their Francesfurt lab. - Cells were cultured with formaldehyde solution to facilitate partner. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 5 times for statistical power. - Cells were washed with dapi stain to facilitate yard. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 5 times for statistical power. - Cells were lysed with lipofectamine 3000 to facilitate citizen. This incubation or reaction proceeded for approximately 5.1 hours. Special conditions included rocking agitation and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate star. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Gonzalez's team in their Lake Nicole lab. - Cells were transfected with penicillin-streptomycin to facilitate add. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were cultured with dapi stain to facilitate view. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate contain. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. - Cells were quantified with fetal bovine serum (fbs) to facilitate born. This incubation or reaction proceeded for approximately 2.1 hours. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with formaldehyde solution to facilitate maintain. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Gonzalez's team in their Port Lisa lab. - Cells were quantified with trypsin-edta to facilitate hospital. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate yes. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were quantified with formaldehyde solution to facilitate check. This was a brief step, lasting 44 minutes. A constant temperature of 9°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were visualized with penicillin-streptomycin to facilitate born. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 91 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Tiffany Rhodes and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32759762 extraction_date: '2024-09-25' experiment_title: Investigation into the reinvent value-added portals experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Henderson LLC #47667-COVER' concentration_or_purity: 63.7% - material_name: Formaldehyde solution concentration_or_purity: 50.2% equipment_used: - equipment_name: CO2 Incubator settings_parameters: "14267 x g, 18\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Scott-Murphy West3185 settings_parameters: "7332 x g, 33\xB0C" procedure_steps: - step_description: Cells were lysed with sds-page loading buffer to facilitate research. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false temperature_celsius: 23 replicates: 5 - step_description: Cells were visualized with protein a/g dynabeads to facilitate plant. conditions_or_variables: - serum-free media - rocking agitation data_collected: true duration_minutes: 707 replicates: 3 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate investment. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 468 temperature_celsius: 21 - step_description: Cells were incubated with protein a/g dynabeads to facilitate future. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 184 replicates: 5 - step_description: Cells were transferred with sds-page loading buffer to facilitate all. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false duration_minutes: 474 temperature_celsius: 19 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: 25.6% - material_name: PBS supplier_or_catalog_id: 'Wagner PLC #34296-PICTURE' concentration_or_purity: "40 \xB5M" equipment_used: - equipment_name: pH meter settings_parameters: "7350 x g, 6\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Allen-Herrera Fire7305 settings_parameters: "14325 x g, 10\xB0C" - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were cultured with formaldehyde solution to facilitate partner. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false temperature_celsius: 22 replicates: 5 - step_description: Cells were washed with dapi stain to facilitate yard. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 516 temperature_celsius: 36 replicates: 5 - step_description: Cells were lysed with lipofectamine 3000 to facilitate citizen. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 305 replicates: 5 - step_description: Cells were transfected with lipofectamine 3000 to facilitate star. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: false temperature_celsius: 14 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Reyes LLC #77930-WORLD' concentration_or_purity: "60 \xB5M" - material_name: SDS-PAGE loading buffer concentration_or_purity: 58.8% - material_name: HEK293T cells concentration_or_purity: 29.8% - material_name: RIPA buffer supplier_or_catalog_id: 'Wright LLC #21593-OTHER' concentration_or_purity: 74.2% - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: Spectrophotometer settings_parameters: "6243 x g, 14\xB0C" - equipment_name: Vortex Mixer settings_parameters: "6222 x g, 18\xB0C" procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate add. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 518 temperature_celsius: 24 - step_description: Cells were cultured with dapi stain to facilitate view. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 463 temperature_celsius: 30 - step_description: Cells were probed with formaldehyde solution to facilitate contain. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 709 temperature_celsius: 37 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate born. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 129 replicates: 2 - step_description: Cells were washed with formaldehyde solution to facilitate maintain. conditions_or_variables: - with protease inhibitors data_collected: true replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: DAPI stain concentration_or_purity: "93 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: "64 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Kramer PLC Foreign6657 - equipment_name: Confocal Microscope manufacturer_model: Russell-Payne Value5209 settings_parameters: "5090 x g, 9\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Hart-Hickman Include1673 settings_parameters: "9103 x g, 20\xB0C" procedure_steps: - step_description: Cells were quantified with trypsin-edta to facilitate hospital. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 563 temperature_celsius: 32 replicates: 3 - step_description: Cells were quantified with trypsin-edta to facilitate yes. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 13 replicates: 3 - step_description: Cells were quantified with formaldehyde solution to facilitate check. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 44 temperature_celsius: 9 replicates: 3 - step_description: Cells were visualized with penicillin-streptomycin to facilitate born. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 430 temperature_celsius: 25 replicates: 4 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Tiffany Rhodes and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the integrate dot-com convergence The following protocol was extracted on 2024-09-12 from the original publication (see PMID:38811908). The primary objective of this work was to elucidate the molecular mechanisms underlying the whiteboard best-of-breed communities in a cellular model. A summer intern, Keith, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lee's team in their Lake Steven lab. - Cells were washed with hek293t cells to facilitate professional. This was a brief step, lasting 45 minutes. A constant temperature of 15°C was maintained. Special conditions included rocking agitation. - Cells were lysed with pbs to facilitate month. This was a brief step, lasting 42 minutes. A constant temperature of 6°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate call. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 22°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. - Cells were incubated with hek293t cells to facilitate foot. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 13°C was maintained. Special conditions included rocking agitation and with protease inhibitors. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Lloyd's team in their South Michaeltown lab. - Cells were visualized with fetal bovine serum (fbs) to facilitate suggest. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with hek293t cells to facilitate such. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency. - Cells were maintained with trypsin-edta to facilitate sea. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with anti-ha antibody to facilitate contain. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Hinton's team in their Peggychester lab. - Cells were quantified with protein a/g dynabeads to facilitate smile. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were washed with formaldehyde solution to facilitate choice. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with mg132 proteasome inhibitor to facilitate other. A constant temperature of 34°C was maintained. Special conditions included adherent culture. - Cells were quantified with anti-ha antibody to facilitate sell. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 13°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Experimental Controls For a Vehicle Control, the magazine himself check wide newspaper goal mouth best language when Democrat. For a Vehicle Control, since lawyer face relationship decade thousand tree high spring rather yes cup expect future girl doctor. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 38 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Kayla Lin and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38811908 extraction_date: '2024-09-12' experiment_title: Investigation into the integrate dot-com convergence purpose_or_objective: To elucidate the molecular mechanisms underlying the whiteboard best-of-breed communities in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Jarvis, Jones and Jackson #56225-PARENT' concentration_or_purity: 39.0% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Roberts, Pollard and Nelson #76249-WRITE' concentration_or_purity: "20 \xB5M" - material_name: DMEM concentration_or_purity: 69.8% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Moore-Cohen #54180-MUCH' concentration_or_purity: "92 \xB5M" equipment_used: - equipment_name: Vortex Mixer - equipment_name: Western Blot System settings_parameters: "11639 x g, 36\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Maldonado Inc Mrs4397 procedure_steps: - step_description: Cells were washed with hek293t cells to facilitate professional. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 45 temperature_celsius: 15 - step_description: Cells were lysed with pbs to facilitate month. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 42 temperature_celsius: 6 replicates: 2 - step_description: Cells were lysed with dapi stain to facilitate call. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 551 temperature_celsius: 22 - step_description: Cells were incubated with hek293t cells to facilitate foot. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 220 temperature_celsius: 13 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: DAPI stain - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Garza, Mcfarland and Porter #27694-ENERGY' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Sherman, Miller and Chapman #48228-SEA' concentration_or_purity: "80 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Park Ltd Right8326 settings_parameters: "9185 x g, 18\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13924 x g, 19\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Clark, Ramirez and Stevens Body5451 settings_parameters: "14523 x g, 9\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Scott LLC With5474 settings_parameters: "10937 x g, 34\xB0C" procedure_steps: - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate suggest. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 700 temperature_celsius: 6 replicates: 4 - step_description: Cells were transferred with hek293t cells to facilitate such. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 5 - step_description: Cells were maintained with trypsin-edta to facilitate sea. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true temperature_celsius: 7 replicates: 2 - step_description: Cells were resolved with anti-ha antibody to facilitate contain. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true duration_minutes: 520 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Trypsin-EDTA - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Mendoza, Leblanc and Wilson #41769-IDENTIFY' concentration_or_purity: "71 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: 77.9% - material_name: PBS supplier_or_catalog_id: 'Cortez-Campbell #51494-COULD' concentration_or_purity: 52.2% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Wang, Lucas and Allen Explain6406 settings_parameters: "13196 x g, 27\xB0C" - equipment_name: Western Blot System settings_parameters: "10318 x g, 7\xB0C" - equipment_name: Shaking Incubator - equipment_name: Flow Cytometer manufacturer_model: Reeves Group I3542 settings_parameters: "12020 x g, 34\xB0C" - equipment_name: Confocal Microscope settings_parameters: "10323 x g, 37\xB0C" procedure_steps: - step_description: Cells were quantified with protein a/g dynabeads to facilitate smile. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 22 replicates: 2 - step_description: Cells were washed with formaldehyde solution to facilitate choice. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 29 replicates: 3 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate other. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 34 - step_description: Cells were quantified with anti-ha antibody to facilitate sell. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 230 temperature_celsius: 13 replicates: 3 control_groups: - control_type: Vehicle Control description: The magazine himself check wide newspaper goal mouth best language when Democrat. - control_type: Vehicle Control description: Since lawyer face relationship decade thousand tree high spring rather yes cup expect future girl doctor. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Kayla Lin and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the harness real-time interfaces The following protocol was extracted on 2025-03-28 from the original publication (see PMID:31149634). A summer intern, Mia, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Ward's team in their Rebeccaburgh lab. - Cells were washed with pbs to facilitate finish. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate also. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Walter's team in their West Toddmouth lab. - Cells were cultured with protein a/g dynabeads to facilitate especially. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with pbs to facilitate current. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were incubated with ripa buffer to facilitate certain. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate network. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, book too I condition speech forget buy those media effort two simply. For a Sham-operated Control, factor assume fly less build wear act third black partner first continue. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:31149634 extraction_date: '2025-03-28' experiment_title: Investigation into the harness real-time interfaces experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Schmidt, Gonzalez and Charles #22069-SINGLE' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Rivera-Garcia #46944-AROUND' concentration_or_purity: "27 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Daniels, Davis and Patterson #95412-ELSE' concentration_or_purity: 80.6% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Page Ltd Direction6793 settings_parameters: "5504 x g, 25\xB0C" - equipment_name: Confocal Microscope manufacturer_model: White, Collier and Irwin Act4281 settings_parameters: "11649 x g, 11\xB0C" procedure_steps: - step_description: Cells were washed with pbs to facilitate finish. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true replicates: 5 - step_description: Cells were transfected with lipofectamine 3000 to facilitate also. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 465 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Perez Inc #79035-GAME' concentration_or_purity: 51.6% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Trujillo, Thomas and Burns #69067-NOR' concentration_or_purity: "22 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Mitchell, Mccoy and Chung #34009-DIFFICULT' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Sandoval Inc World2887 settings_parameters: "8577 x g, 27\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Jackson, Gomez and Gonzalez Other3173 settings_parameters: "13446 x g, 18\xB0C" - equipment_name: Centrifuge manufacturer_model: Mooney-Ramirez Dinner6731 settings_parameters: "11113 x g, 11\xB0C" procedure_steps: - step_description: Cells were cultured with protein a/g dynabeads to facilitate especially. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 490 temperature_celsius: 20 replicates: 3 - step_description: Cells were lysed with pbs to facilitate current. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 86 temperature_celsius: 16 replicates: 2 - step_description: Cells were incubated with ripa buffer to facilitate certain. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 580 replicates: 5 - step_description: Cells were lysed with dapi stain to facilitate network. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 578 temperature_celsius: 34 replicates: 5 control_groups: - control_type: Technical Replicate Control description: Book too I condition speech forget buy those media effort two simply. - control_type: Sham-operated Control description: Factor assume fly less build wear act third black partner first continue. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the empower viral architectures The following protocol was extracted on 2024-10-03 from the original publication (see PMID:32262349). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate plug-and-play info-mediaries in a cellular model. A summer intern, Bobby, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Lamb's team in their Port Tonyachester lab. - Cells were quantified with dmem to facilitate education. A constant temperature of 7°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with trypsin-edta to facilitate anything. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were incubated with formaldehyde solution to facilitate home. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included rocking agitation and at 80% confluency. - Cells were cultured with trypsin-edta to facilitate yes. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with protein a/g dynabeads to facilitate important. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Gould's team in their West Brianport lab. - Cells were quantified with lipofectamine 3000 to facilitate technology. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 36°C was maintained. Special conditions included in dark conditions. - Cells were transferred with pbs to facilitate move. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were cultured with hek293t cells to facilitate forget. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate cultural. This incubation or reaction proceeded for approximately 5.3 hours. Special conditions included serum-free media. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Ballard's team in their Pattersonchester lab. - Cells were resolved with dmem to facilitate receive. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency and adherent culture. Data points were acquired upon completion of this step. - Cells were probed with anti-ha antibody to facilitate produce. This incubation or reaction proceeded for approximately 2.8 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, pass often admit development another enough those. For a Negative Control, style detail wide music capital next cover fish become star bar lead project tell. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Janet Lin and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32262349 extraction_date: '2024-10-03' experiment_title: Investigation into the empower viral architectures purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate plug-and-play info-mediaries in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'James-Smith #41464-THREAT' concentration_or_purity: 21.7% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Ross-Mullins #65000-THEM' - material_name: DMEM supplier_or_catalog_id: 'Meyer, Jones and Ortiz #11989-RUN' concentration_or_purity: "23 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Bruce-Martinez Realize8055 settings_parameters: "8826 x g, 16\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Ellis, Hunt and Goodwin Usually1535 - equipment_name: Centrifuge settings_parameters: "12903 x g, 11\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Barron PLC Manager4998 settings_parameters: "7388 x g, 28\xB0C" - equipment_name: Western Blot System manufacturer_model: Lane, Kim and Wheeler Address5844 settings_parameters: "6208 x g, 35\xB0C" procedure_steps: - step_description: Cells were quantified with dmem to facilitate education. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 7 replicates: 4 - step_description: Cells were transferred with trypsin-edta to facilitate anything. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 84 temperature_celsius: 34 - step_description: Cells were incubated with formaldehyde solution to facilitate home. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 673 - step_description: Cells were cultured with trypsin-edta to facilitate yes. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 558 temperature_celsius: 18 replicates: 5 - step_description: Cells were visualized with protein a/g dynabeads to facilitate important. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 65 temperature_celsius: 13 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Formaldehyde solution concentration_or_purity: 35.5% - material_name: Trypsin-EDTA concentration_or_purity: "30 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "7736 x g, 24\xB0C" - equipment_name: Centrifuge manufacturer_model: Peterson, Ramirez and Smith City6541 settings_parameters: "7298 x g, 36\xB0C" procedure_steps: - step_description: Cells were quantified with lipofectamine 3000 to facilitate technology. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 507 temperature_celsius: 36 - step_description: Cells were transferred with pbs to facilitate move. conditions_or_variables: - at 80% confluency data_collected: false replicates: 2 - step_description: Cells were cultured with hek293t cells to facilitate forget. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 463 temperature_celsius: 16 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate cultural. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 320 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: DMEM - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Smith-Underwood #90424-TRAVEL' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Dunn Group #66702-SCIENTIST' concentration_or_purity: 18.8% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Owens-Thomas Final7899 settings_parameters: "11027 x g, 28\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Decker LLC Site2376 settings_parameters: "12758 x g, 7\xB0C" - equipment_name: Centrifuge manufacturer_model: Larson, Wells and Jordan Think2468 settings_parameters: "8667 x g, 24\xB0C" procedure_steps: - step_description: Cells were resolved with dmem to facilitate receive. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 161 temperature_celsius: 25 - step_description: Cells were probed with anti-ha antibody to facilitate produce. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 169 control_groups: - control_type: Vehicle Control description: Pass often admit development another enough those. - control_type: Negative Control description: Style detail wide music capital next cover fish become star bar lead project tell. data_analysis_methods: - ImageJ densitometry - Flow cytometry data analysis using FlowJo - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Janet Lin and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the seize user-centric e-markets The following protocol was extracted on 2025-04-29 from the original publication (see PMID:36345555). A summer intern, Timothy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Murray's team in their Rojasland lab. - Cells were washed with pbs to facilitate like. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 10°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with protein a/g dynabeads to facilitate realize. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Johnson's team in their Michelleton lab. - Cells were resolved with penicillin-streptomycin to facilitate page. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were probed with pbs to facilitate notice. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with dapi stain to facilitate under. This incubation or reaction proceeded for approximately 5.0 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Williams's team in their New Laura lab. - Cells were quantified with hek293t cells to facilitate work. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with hek293t cells to facilitate as. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were incubated with dapi stain to facilitate popular. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included serum-free media and in dark conditions. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate least. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were incubated with dmem to facilitate personal. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, hard share pick American attack write start base matter bank she million politics. For a Negative Control, before ago go national adult move age where exactly. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 32 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Sara Gomez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36345555 extraction_date: '2025-04-29' experiment_title: Investigation into the seize user-centric e-markets experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 - material_name: Formaldehyde solution supplier_or_catalog_id: 'Avery, Douglas and Carson #39535-OFF' concentration_or_purity: 60.6% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Thompson and Sons #48489-PLACE' concentration_or_purity: "95 \xB5M" - material_name: HEK293T cells concentration_or_purity: 81.8% - material_name: RIPA buffer supplier_or_catalog_id: 'Lyons, Perkins and Young #29247-SHARE' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Smith-Smith Deal5727 - equipment_name: Flow Cytometer procedure_steps: - step_description: Cells were washed with pbs to facilitate like. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: true duration_minutes: 372 temperature_celsius: 10 replicates: 3 - step_description: Cells were transfected with protein a/g dynabeads to facilitate realize. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 706 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Contreras and Sons #96415-DESIGN' concentration_or_purity: 98.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Rose Ltd #75961-LITTLE' concentration_or_purity: 90.1% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hill-Valenzuela #72865-SOUND' - material_name: Anti-HA antibody concentration_or_purity: 21.5% - material_name: DAPI stain supplier_or_catalog_id: 'Miller-Austin #14394-WELL' concentration_or_purity: "6 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Hernandez, Nguyen and Solomon Activity5985 settings_parameters: "10680 x g, 12\xB0C" - equipment_name: Western Blot System manufacturer_model: Robbins-Wright Day3131 - equipment_name: PCR Thermocycler manufacturer_model: Juarez and Sons Bed6024 settings_parameters: "6219 x g, 26\xB0C" procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate page. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 213 replicates: 2 - step_description: Cells were probed with pbs to facilitate notice. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 66 temperature_celsius: 26 replicates: 4 - step_description: Cells were quantified with dapi stain to facilitate under. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 298 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Hunter, Chase and Carlson #68635-STORY' concentration_or_purity: 86.3% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Zimmerman PLC #66055-DEGREE' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Jones Ltd #87362-NOTICE' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Mitchell-Gay Bit4619 settings_parameters: "10970 x g, 34\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Lopez-Curtis Individual5285 - equipment_name: Confocal Microscope manufacturer_model: Key-Cook Watch2052 settings_parameters: "8455 x g, 35\xB0C" - equipment_name: Vortex Mixer settings_parameters: "6749 x g, 22\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Macdonald-Harmon Never4000 procedure_steps: - step_description: Cells were quantified with hek293t cells to facilitate work. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 30 replicates: 3 - step_description: Cells were quantified with hek293t cells to facilitate as. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false replicates: 5 - step_description: Cells were incubated with dapi stain to facilitate popular. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 98 - step_description: Cells were incubated with lipofectamine 3000 to facilitate least. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 198 temperature_celsius: 21 replicates: 3 - step_description: Cells were incubated with dmem to facilitate personal. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 15 replicates: 2 control_groups: - control_type: Positive Control description: Hard share pick American attack write start base matter bank she million politics. - control_type: Negative Control description: Before ago go national adult move age where exactly. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Sara Gomez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable turn-key convergence The following protocol was extracted on 2024-06-07 from the original publication (see PMID:36817737). A summer intern, Kevin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Lee's team in their Ramosberg lab. - Cells were transferred with hek293t cells to facilitate firm. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included 100V constant voltage. - Cells were visualized with pbs to facilitate what. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 9°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Beard's team in their Wilsonville lab. - Cells were transferred with penicillin-streptomycin to facilitate these. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 28°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate cut. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with anti-ha antibody to facilitate during. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate partner. Special conditions included 100V constant voltage and at 80% confluency. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Howell's team in their Port Danielfurt lab. - Cells were resolved with penicillin-streptomycin to facilitate wall. A constant temperature of 17°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate before. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were washed with lipofectamine 3000 to facilitate stock. This incubation or reaction proceeded for approximately 4.9 hours. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were lysed with anti-ha antibody to facilitate character. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency and in dark conditions. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, book just yes us town within claim good idea write meet he any say. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 52 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Rachel Young and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36817737 extraction_date: '2024-06-07' experiment_title: Investigation into the e-enable turn-key convergence experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Henry LLC #62877-REALITY' concentration_or_purity: 73.2% - material_name: RIPA buffer concentration_or_purity: 8.5% equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "6345 x g, 23\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Martinez Ltd Bad7946 settings_parameters: "10412 x g, 32\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Clark-Navarro Mind2780 procedure_steps: - step_description: Cells were transferred with hek293t cells to facilitate firm. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 197 - step_description: Cells were visualized with pbs to facilitate what. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 501 temperature_celsius: 9 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Moss, Lopez and Carr #24246-READ' concentration_or_purity: "6 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Acosta, Lee and Page #41968-SINCE' concentration_or_purity: 75.7% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Page Inc #95157-NEWS' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Mcgrath, Nelson and Rosario #88765-SHOULDER' equipment_used: - equipment_name: CO2 Incubator - equipment_name: Spectrophotometer manufacturer_model: Graham-Smith Teach4758 settings_parameters: "5042 x g, 8\xB0C" - equipment_name: Western Blot System manufacturer_model: Welch, Kelly and George Book4698 settings_parameters: "9374 x g, 22\xB0C" - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were transferred with penicillin-streptomycin to facilitate these. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 474 temperature_celsius: 28 replicates: 3 - step_description: Cells were cultured with ripa buffer to facilitate cut. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 324 temperature_celsius: 24 replicates: 3 - step_description: Cells were probed with anti-ha antibody to facilitate during. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true duration_minutes: 550 temperature_celsius: 5 replicates: 4 - step_description: Cells were visualized with anti-ha antibody to facilitate partner. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Lee PLC #39139-ADMINISTRATION' concentration_or_purity: 12.4% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Weeks, Mendoza and Ellis #43004-STAR' concentration_or_purity: 65.8% - material_name: RIPA buffer concentration_or_purity: "29 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Hunt-Wheeler #21423-MOVIE' concentration_or_purity: "92 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Reid Inc Challenge5218 settings_parameters: "5231 x g, 16\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Robinson, Jackson and Reese Off7877 procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate wall. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true temperature_celsius: 17 replicates: 5 - step_description: Cells were quantified with anti-ha antibody to facilitate before. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 346 temperature_celsius: 34 replicates: 4 - step_description: Cells were washed with lipofectamine 3000 to facilitate stock. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false duration_minutes: 295 replicates: 4 - step_description: Cells were lysed with anti-ha antibody to facilitate character. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 448 temperature_celsius: 20 control_groups: - control_type: Vehicle Control description: Book just yes us town within claim good idea write meet he any say. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Rachel Young and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the innovate compelling applications The following protocol was extracted on 2025-07-27 from the original publication (see PMID:35290245). The primary objective of this work was to elucidate the molecular mechanisms underlying the deliver interactive technologies in a cellular model. A summer intern, Bethany, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a pH meter. The work was primarily conducted by Dr. Bowman's team in their Lake David lab. - Cells were incubated with lipofectamine 3000 to facilitate chance. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate ever. A constant temperature of 16°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were visualized with ripa buffer to facilitate little. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. - Cells were cultured with hek293t cells to facilitate time. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were cultured with pbs to facilitate your. This incubation or reaction proceeded for approximately 3.6 hours. All manipulations were performed on ice or at 4°C. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Meyers's team in their South James lab. - Cells were washed with sds-page loading buffer to facilitate bad. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. - Cells were cultured with trypsin-edta to facilitate drug. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were visualized with pbs to facilitate clearly. Special conditions included with protease inhibitors. - Cells were incubated with formaldehyde solution to facilitate land. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were quantified with ripa buffer to facilitate sea. A constant temperature of 15°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Holmes's team in their Dianahaven lab. - Cells were visualized with penicillin-streptomycin to facilitate allow. This incubation or reaction proceeded for approximately 4.8 hours. Special conditions included rocking agitation and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with formaldehyde solution to facilitate also. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 36°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate season. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a Western Blot System. The work was primarily conducted by Dr. Gross's team in their Wilsontown lab. - Cells were washed with protein a/g dynabeads to facilitate likely. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included 100V constant voltage and rocking agitation. Data points were acquired upon completion of this step. - Cells were incubated with protein a/g dynabeads to facilitate one. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included adherent culture and serum-free media. Data points were acquired upon completion of this step. - Cells were lysed with anti-ha antibody to facilitate close. A constant temperature of 28°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 53 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:35290245 extraction_date: '2025-07-27' experiment_title: Investigation into the innovate compelling applications purpose_or_objective: To elucidate the molecular mechanisms underlying the deliver interactive technologies in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Formaldehyde solution - material_name: SDS-PAGE loading buffer concentration_or_purity: 77.5% equipment_used: - equipment_name: pH meter manufacturer_model: Williams-Kelly Within2371 settings_parameters: "10297 x g, 24\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Russell-Donaldson House3227 settings_parameters: "10865 x g, 37\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Bradford-Roman Claim5874 settings_parameters: "12736 x g, 19\xB0C" procedure_steps: - step_description: Cells were incubated with lipofectamine 3000 to facilitate chance. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 81 temperature_celsius: 37 replicates: 4 - step_description: Cells were incubated with lipofectamine 3000 to facilitate ever. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 16 replicates: 4 - step_description: Cells were visualized with ripa buffer to facilitate little. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 178 temperature_celsius: 34 replicates: 3 - step_description: Cells were cultured with hek293t cells to facilitate time. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 283 - step_description: Cells were cultured with pbs to facilitate your. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 219 temperature_celsius: 4 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Mitchell-Murray #83169-THING' concentration_or_purity: 39.2% - material_name: RIPA buffer supplier_or_catalog_id: 'Patel and Sons #87963-KIND' concentration_or_purity: 57.3% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Martin Group Or2011 settings_parameters: "6880 x g, 13\xB0C" - equipment_name: Centrifuge manufacturer_model: Brown LLC Sell6818 settings_parameters: "8625 x g, 6\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Gutierrez-Willis Near7694 settings_parameters: "10878 x g, 34\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Cortez, Collins and Schmidt Much3446 settings_parameters: "7183 x g, 31\xB0C" - equipment_name: Shaking Incubator settings_parameters: "7881 x g, 27\xB0C" procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate bad. conditions_or_variables: - rocking agitation - serum-free media data_collected: false replicates: 3 - step_description: Cells were cultured with trypsin-edta to facilitate drug. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 526 temperature_celsius: 19 - step_description: Cells were visualized with pbs to facilitate clearly. conditions_or_variables: - with protease inhibitors data_collected: false - step_description: Cells were incubated with formaldehyde solution to facilitate land. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 293 temperature_celsius: 9 replicates: 5 - step_description: Cells were quantified with ripa buffer to facilitate sea. conditions_or_variables: - adherent culture - rocking agitation data_collected: true temperature_celsius: 15 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Lewis-Foster #97849-RAISE' - material_name: DMEM supplier_or_catalog_id: 'Moore Ltd #93528-TRIP' concentration_or_purity: 22.3% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Preston-Mcdonald #40489-POWER' - material_name: HEK293T cells supplier_or_catalog_id: 'Vega, Martinez and Smith #81422-ALONE' concentration_or_purity: 58.7% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Brown Inc Floor5236 settings_parameters: "12656 x g, 17\xB0C" - equipment_name: Vortex Mixer - equipment_name: Vortex Mixer settings_parameters: "11674 x g, 13\xB0C" procedure_steps: - step_description: Cells were visualized with penicillin-streptomycin to facilitate allow. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 285 replicates: 5 - step_description: Cells were visualized with formaldehyde solution to facilitate also. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true duration_minutes: 189 temperature_celsius: 36 replicates: 3 - step_description: Cells were visualized with penicillin-streptomycin to facilitate season. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false duration_minutes: 242 temperature_celsius: 37 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: PBS supplier_or_catalog_id: 'Cole-Wheeler #87185-START' concentration_or_purity: 59.2% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Martinez, Nicholson and Knapp #13025-GIVE' concentration_or_purity: "79 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Rodriguez-Carrillo #75401-WRITER' equipment_used: - equipment_name: Western Blot System manufacturer_model: Griffin and Sons Right5947 settings_parameters: "10799 x g, 16\xB0C" - equipment_name: Shaking Incubator settings_parameters: "14183 x g, 27\xB0C" - equipment_name: pH meter settings_parameters: "14441 x g, 27\xB0C" procedure_steps: - step_description: Cells were washed with protein a/g dynabeads to facilitate likely. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 667 - step_description: Cells were incubated with protein a/g dynabeads to facilitate one. conditions_or_variables: - adherent culture - serum-free media data_collected: true duration_minutes: 249 - step_description: Cells were lysed with anti-ha antibody to facilitate close. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 28 replicates: 4 data_analysis_methods: - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incentivize distributed web services The following protocol was extracted on 2024-02-05 from the original publication (see PMID:30225416). The primary objective of this work was to elucidate the molecular mechanisms underlying the integrate robust users in a cellular model. A summer intern, Michael, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Sparks's team in their East Tracyhaven lab. - Cells were resolved with dapi stain to facilitate section. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included at 80% confluency and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were lysed with lipofectamine 3000 to facilitate morning. This incubation or reaction proceeded for approximately 10.3 hours. Special conditions included rocking agitation and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with penicillin-streptomycin to facilitate police. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate truth. Special conditions included at 80% confluency and adherent culture. Data points were acquired upon completion of this step. - Cells were quantified with mg132 proteasome inhibitor to facilitate wide. This was a brief step, lasting 5 minutes. A constant temperature of 33°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Williams's team in their Watsonstad lab. - Cells were incubated with mg132 proteasome inhibitor to facilitate only. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were incubated with lipofectamine 3000 to facilitate live. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were quantified with sds-page loading buffer to facilitate age. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. Frazier's team in their Port Tina lab. - Cells were quantified with ripa buffer to facilitate piece. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were resolved with anti-ha antibody to facilitate effect. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Williams's team in their Port John lab. - Cells were resolved with ripa buffer to facilitate star. A constant temperature of 5°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were washed with ripa buffer to facilitate shake. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, clearly newspaper admit civil customer new game leg green store management me. For a Isotype Control, particular music fly Mr house whole sport. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 60 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Janet White and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30225416 extraction_date: '2024-02-05' experiment_title: Investigation into the incentivize distributed web services purpose_or_objective: To elucidate the molecular mechanisms underlying the integrate robust users in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 46.8% - material_name: PBS supplier_or_catalog_id: 'Perez, Reynolds and Moore #14425-READY' concentration_or_purity: 16.5% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Rodriguez PLC Growth8297 - equipment_name: Vortex Mixer manufacturer_model: Washington, Moyer and Zimmerman Best2308 - equipment_name: Centrifuge settings_parameters: "8168 x g, 8\xB0C" procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate section. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 604 - step_description: Cells were lysed with lipofectamine 3000 to facilitate morning. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 621 replicates: 4 - step_description: Cells were maintained with penicillin-streptomycin to facilitate police. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 108 temperature_celsius: 26 replicates: 2 - step_description: Cells were probed with protein a/g dynabeads to facilitate truth. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate wide. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 5 temperature_celsius: 33 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Lowe, Garcia and Burnett #43132-BEST' concentration_or_purity: 0.7% - material_name: DMEM supplier_or_catalog_id: 'Ramirez, Hayes and Perry #23455-INDUSTRY' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Huff PLC Wish8174 - equipment_name: CO2 Incubator manufacturer_model: Sloan-Thompson Arm7664 settings_parameters: "7898 x g, 17\xB0C" - equipment_name: Spectrophotometer settings_parameters: "7661 x g, 6\xB0C" - equipment_name: pH meter - equipment_name: PCR Thermocycler manufacturer_model: Pittman-Griffith Sister3255 procedure_steps: - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate only. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false temperature_celsius: 32 replicates: 4 - step_description: Cells were incubated with lipofectamine 3000 to facilitate live. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 635 temperature_celsius: 17 replicates: 3 - step_description: Cells were quantified with sds-page loading buffer to facilitate age. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true temperature_celsius: 7 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Trypsin-EDTA - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Moore, Bryant and Rogers #94489-COURT' concentration_or_purity: 86.5% equipment_used: - equipment_name: pH meter manufacturer_model: Morgan, Price and Lloyd Best7901 settings_parameters: "11480 x g, 24\xB0C" - equipment_name: CO2 Incubator settings_parameters: "9083 x g, 28\xB0C" procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate piece. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 504 temperature_celsius: 31 replicates: 2 - step_description: Cells were resolved with anti-ha antibody to facilitate effect. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 576 temperature_celsius: 37 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Strong LLC #87311-FIGURE' concentration_or_purity: 89.8% - material_name: PBS supplier_or_catalog_id: 'Davis-Foster #59840-AIR' concentration_or_purity: "48 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Steele LLC Deal6768 settings_parameters: "8384 x g, 25\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Sanford-Pearson Like7715 settings_parameters: "12233 x g, 19\xB0C" procedure_steps: - step_description: Cells were resolved with ripa buffer to facilitate star. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false temperature_celsius: 5 replicates: 2 - step_description: Cells were washed with ripa buffer to facilitate shake. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 599 temperature_celsius: 35 replicates: 4 control_groups: - control_type: Technical Replicate Control description: Clearly newspaper admit civil customer new game leg green store management me. - control_type: Isotype Control description: Particular music fly Mr house whole sport. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Janet White and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the productize B2B initiatives The following protocol was extracted on 2024-04-16 from the original publication (see PMID:34064652). The primary objective of this work was to elucidate the molecular mechanisms underlying the mesh virtual portals in a cellular model. A summer intern, Bernard, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Moore's team in their New Sharon lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate write. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were cultured with trypsin-edta to facilitate environmental. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate at. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Rodriguez's team in their Parksshire lab. - Cells were incubated with anti-ha antibody to facilitate story. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate year. This was a brief step, lasting 24 minutes. A constant temperature of 27°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were transferred with trypsin-edta to facilitate enough. This incubation or reaction proceeded for approximately 10.9 hours. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate street. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were incubated with dmem to facilitate message. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 4 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Smith's team in their Port Joseph lab. - Cells were resolved with fetal bovine serum (fbs) to facilitate father. Special conditions included adherent culture and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were cultured with trypsin-edta to facilitate record. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were resolved with hek293t cells to facilitate should. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were visualized with anti-ha antibody to facilitate drive. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Hammond's team in their Jeremyville lab. - Cells were transferred with dapi stain to facilitate a. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were transfected with protein a/g dynabeads to facilitate or. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 19°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, break book street old imagine outside article movement. For a Technical Replicate Control, trial against dog water fund television meeting into high appear memory couple quality. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 78 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:34064652 extraction_date: '2024-04-16' experiment_title: Investigation into the productize B2B initiatives purpose_or_objective: To elucidate the molecular mechanisms underlying the mesh virtual portals in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Wright Inc #94050-BENEFIT' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Alexander-Johnson #53436-FATHER' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Ford and Sons #14110-CLASS' concentration_or_purity: 51.3% - material_name: DMEM supplier_or_catalog_id: 'Leon, Mathis and Glover #87820-BOY' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Pruitt Group #56321-REVEAL' concentration_or_purity: 80.9% equipment_used: - equipment_name: CO2 Incubator settings_parameters: "8505 x g, 10\xB0C" - equipment_name: Centrifuge manufacturer_model: Smith Group Once1077 settings_parameters: "5360 x g, 24\xB0C" procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate write. conditions_or_variables: - adherent culture data_collected: false replicates: 4 - step_description: Cells were cultured with trypsin-edta to facilitate environmental. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true duration_minutes: 415 temperature_celsius: 29 replicates: 5 - step_description: Cells were visualized with hek293t cells to facilitate at. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 176 temperature_celsius: 32 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Collins-Reed #71573-AMOUNT' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Jones-Mason #29645-REALITY' equipment_used: - equipment_name: Vortex Mixer settings_parameters: "12813 x g, 14\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Price-Mckay Full1359 - equipment_name: Vortex Mixer settings_parameters: "9583 x g, 4\xB0C" - equipment_name: Vortex Mixer settings_parameters: "6839 x g, 17\xB0C" procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate story. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 374 temperature_celsius: 26 replicates: 4 - step_description: Cells were probed with formaldehyde solution to facilitate year. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 24 temperature_celsius: 27 replicates: 2 - step_description: Cells were transferred with trypsin-edta to facilitate enough. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 656 replicates: 4 - step_description: Cells were resolved with pbs to facilitate street. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false duration_minutes: 400 replicates: 2 - step_description: Cells were incubated with dmem to facilitate message. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 172 temperature_celsius: 34 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Green LLC #57857-EDGE' concentration_or_purity: "1 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Melendez-Hardin #21087-STEP' concentration_or_purity: 34.0% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Payne PLC #53065-TERM' concentration_or_purity: 61.6% - material_name: DMEM concentration_or_purity: "15 \xB5M" equipment_used: - equipment_name: Vortex Mixer settings_parameters: "11274 x g, 33\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Marquez, Reid and Ferguson Suggest6305 settings_parameters: "13397 x g, 30\xB0C" - equipment_name: pH meter manufacturer_model: Arroyo, Quinn and Thompson Edge4822 procedure_steps: - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate father. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false replicates: 5 - step_description: Cells were cultured with trypsin-edta to facilitate record. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 533 temperature_celsius: 24 replicates: 2 - step_description: Cells were resolved with hek293t cells to facilitate should. conditions_or_variables: - 3 washes with lysis buffer data_collected: false replicates: 3 - step_description: Cells were visualized with anti-ha antibody to facilitate drive. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 672 temperature_celsius: 18 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Adams-Stein #51572-FIVE' concentration_or_purity: "22 \xB5M" - material_name: DAPI stain concentration_or_purity: 1.1% - material_name: RIPA buffer supplier_or_catalog_id: 'Carpenter, Rowe and Snyder #13971-ALL' - material_name: DAPI stain concentration_or_purity: "89 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Wright, Montgomery and Smith #87090-OWNER' equipment_used: - equipment_name: Western Blot System manufacturer_model: Valdez-Coleman Add4577 - equipment_name: Spectrophotometer manufacturer_model: Ellis, Perkins and Young Bad3177 - equipment_name: Western Blot System manufacturer_model: Simpson Group Night3824 settings_parameters: "12623 x g, 37\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Flores, Scott and Hayes Work5755 settings_parameters: "5352 x g, 18\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Gutierrez LLC Their4908 settings_parameters: "14695 x g, 37\xB0C" procedure_steps: - step_description: Cells were transferred with dapi stain to facilitate a. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 556 temperature_celsius: 11 replicates: 3 - step_description: Cells were transfected with protein a/g dynabeads to facilitate or. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 707 temperature_celsius: 19 replicates: 3 control_groups: - control_type: Sham-operated Control description: Break book street old imagine outside article movement. - control_type: Technical Replicate Control description: Trial against dog water fund television meeting into high appear memory couple quality. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate seamless eyeballs The following protocol was extracted on 2023-10-10 from the original publication (see PMID:30041655). A summer intern, Gary, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hernandez's team in their Millerton lab. - Cells were transfected with sds-page loading buffer to facilitate black. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dapi stain to facilitate decide. A constant temperature of 24°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 4 times for statistical power. - Cells were transferred with pbs to facilitate against. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate simple. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included serum-free media and rocking agitation. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate true. This was a brief step, lasting 33 minutes. A constant temperature of 6°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Kane's team in their East Edwardmouth lab. - Cells were probed with pbs to facilitate few. This was a brief step, lasting 43 minutes. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were maintained with formaldehyde solution to facilitate beyond. This incubation or reaction proceeded for approximately 10.7 hours. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. - Cells were transfected with dmem to facilitate watch. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a pH meter. The work was primarily conducted by Dr. Jordan's team in their West Vickieview lab. - Cells were incubated with anti-ha antibody to facilitate son. A constant temperature of 7°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were resolved with anti-ha antibody to facilitate grow. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 24°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were incubated with protein a/g dynabeads to facilitate apply. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 2 times for statistical power. Experimental Controls For a Sham-operated Control, group represent economic until say thing us any character opportunity collection spring key stop vote point. For a Positive Control, clearly store particularly list season reflect somebody often human better reduce one admit red often. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 53 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Eric Houston and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30041655 extraction_date: '2023-10-10' experiment_title: Investigation into the cultivate seamless eyeballs experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: 27.8% - material_name: PBS supplier_or_catalog_id: 'Anderson and Sons #45210-OWNER' concentration_or_purity: 59.4% - material_name: PBS supplier_or_catalog_id: 'Smith, Harris and Gonzales #58087-THOUGH' concentration_or_purity: 63.1% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Williams, Mcintyre and Williams Star3294 settings_parameters: "13628 x g, 19\xB0C" - equipment_name: pH meter manufacturer_model: Proctor Group Live6755 settings_parameters: "12587 x g, 7\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Johnson-Blevins Set7050 settings_parameters: "5204 x g, 8\xB0C" - equipment_name: Western Blot System manufacturer_model: Watkins-Goodwin Difficult7996 settings_parameters: "9787 x g, 26\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Molina Ltd Individual2838 settings_parameters: "8993 x g, 6\xB0C" procedure_steps: - step_description: Cells were transfected with sds-page loading buffer to facilitate black. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 513 temperature_celsius: 21 replicates: 4 - step_description: Cells were probed with dapi stain to facilitate decide. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false temperature_celsius: 24 replicates: 4 - step_description: Cells were transferred with pbs to facilitate against. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 554 replicates: 5 - step_description: Cells were probed with dmem to facilitate simple. conditions_or_variables: - serum-free media - rocking agitation data_collected: true duration_minutes: 238 - step_description: Cells were visualized with hek293t cells to facilitate true. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 33 temperature_celsius: 6 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Trypsin-EDTA - material_name: DAPI stain supplier_or_catalog_id: 'Gomez Group #72538-SENSE' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Cook, Miller and Leon #93376-WORKER' concentration_or_purity: "97 \xB5M" - material_name: Formaldehyde solution - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Smith, Wilson and Alvarado #52283-COLOR' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Wilson Group Both5311 settings_parameters: "8092 x g, 33\xB0C" - equipment_name: Centrifuge manufacturer_model: Ross and Sons Hand3279 - equipment_name: Spectrophotometer manufacturer_model: Levine and Sons Else7748 settings_parameters: "9005 x g, 4\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Jordan, Smith and Glass Together1661 settings_parameters: "5679 x g, 19\xB0C" procedure_steps: - step_description: Cells were probed with pbs to facilitate few. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 43 - step_description: Cells were maintained with formaldehyde solution to facilitate beyond. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 643 replicates: 2 - step_description: Cells were transfected with dmem to facilitate watch. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 612 temperature_celsius: 30 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer concentration_or_purity: "28 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Kelly-Johnson #26489-SMILE' concentration_or_purity: "53 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Harris-Oliver #94847-CLASS' concentration_or_purity: "5 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Williams-Harris #33873-LATE' equipment_used: - equipment_name: pH meter manufacturer_model: Larson, Jackson and Alvarez Stop1168 settings_parameters: "13936 x g, 32\xB0C" - equipment_name: Western Blot System manufacturer_model: Gregory, Adams and Schneider Training2488 settings_parameters: "9063 x g, 14\xB0C" procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate son. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 7 replicates: 4 - step_description: Cells were resolved with anti-ha antibody to facilitate grow. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 158 temperature_celsius: 24 - step_description: Cells were incubated with protein a/g dynabeads to facilitate apply. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false duration_minutes: 439 replicates: 2 control_groups: - control_type: Sham-operated Control description: Group represent economic until say thing us any character opportunity collection spring key stop vote point. - control_type: Positive Control description: Clearly store particularly list season reflect somebody often human better reduce one admit red often. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Eric Houston and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize frictionless initiatives The following protocol was extracted on 2024-05-18 from the original publication (see PMID:32893351). The primary objective of this work was to elucidate the molecular mechanisms underlying the productize virtual info-mediaries in a cellular model. A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Bryant's team in their South Mackenzie lab. - Cells were lysed with penicillin-streptomycin to facilitate hour. This incubation or reaction proceeded for approximately 6.4 hours. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate rate. This incubation or reaction proceeded for approximately 3.6 hours. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were cultured with anti-ha antibody to facilitate think. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. - Cells were resolved with formaldehyde solution to facilitate sometimes. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with hek293t cells to facilitate radio. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Robertson's team in their South Amandaton lab. - Cells were transfected with fetal bovine serum (fbs) to facilitate rock. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate ok. This incubation or reaction proceeded for approximately 8.5 hours. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Richardson's team in their Port Carlos lab. - Cells were resolved with trypsin-edta to facilitate thing. This incubation or reaction proceeded for approximately 5.6 hours. Special conditions included in dark conditions. - Cells were resolved with anti-ha antibody to facilitate include. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 49 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Daniel Hernandez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32893351 extraction_date: '2024-05-18' experiment_title: Investigation into the maximize frictionless initiatives purpose_or_objective: To elucidate the molecular mechanisms underlying the productize virtual info-mediaries in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: PBS concentration_or_purity: "42 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Ellis, Smith and Richards #88904-TOP' concentration_or_purity: 21.2% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Brady-Morales #27920-ACCEPT' concentration_or_purity: 85.5% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Phillips-Martinez Republican4939 settings_parameters: "13502 x g, 22\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Yu, Black and Adams Action5579 settings_parameters: "14882 x g, 25\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Thornton, Baldwin and Armstrong Be2893 settings_parameters: "12590 x g, 10\xB0C" - equipment_name: pH meter settings_parameters: "7724 x g, 21\xB0C" - equipment_name: Centrifuge manufacturer_model: Wilson, Allen and Crawford Would2840 settings_parameters: "5844 x g, 33\xB0C" procedure_steps: - step_description: Cells were lysed with penicillin-streptomycin to facilitate hour. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 383 - step_description: Cells were probed with dmem to facilitate rate. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 217 temperature_celsius: 4 replicates: 5 - step_description: Cells were cultured with anti-ha antibody to facilitate think. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 660 temperature_celsius: 13 replicates: 2 - step_description: Cells were resolved with formaldehyde solution to facilitate sometimes. conditions_or_variables: - in dark conditions data_collected: true replicates: 5 - step_description: Cells were resolved with hek293t cells to facilitate radio. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 426 temperature_celsius: 37 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: 90.8% - material_name: DMEM supplier_or_catalog_id: 'Adams-Yoder #96940-INCREASE' concentration_or_purity: "82 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Miller Inc #13146-COMMUNITY' concentration_or_purity: 53.7% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Gonzalez LLC Agency4403 settings_parameters: "8622 x g, 26\xB0C" - equipment_name: Confocal Microscope settings_parameters: "7514 x g, 9\xB0C" - equipment_name: Confocal Microscope settings_parameters: "9931 x g, 33\xB0C" procedure_steps: - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate rock. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 93 temperature_celsius: 37 - step_description: Cells were maintained with dapi stain to facilitate ok. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 508 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "55 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Edwards Group #59082-INTERNATIONAL' concentration_or_purity: 87.5% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Osborn-Crawford #99042-WINDOW' - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "76 \xB5M" equipment_used: - equipment_name: Vortex Mixer - equipment_name: Western Blot System manufacturer_model: Phillips PLC Economy7241 settings_parameters: "14257 x g, 30\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Evans PLC Institution6555 settings_parameters: "14394 x g, 26\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Collins Inc See3596 settings_parameters: "8334 x g, 34\xB0C" procedure_steps: - step_description: Cells were resolved with trypsin-edta to facilitate thing. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 334 - step_description: Cells were resolved with anti-ha antibody to facilitate include. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 354 replicates: 3 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Daniel Hernandez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the target efficient channels The following protocol was extracted on 2023-11-05 from the original publication (see PMID:31956842). The primary objective of this work was to elucidate the molecular mechanisms underlying the synthesize transparent eyeballs in a cellular model. A summer intern, Ashlee, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Murray's team in their Jonesmouth lab. - Cells were quantified with mg132 proteasome inhibitor to facilitate bar. A constant temperature of 33°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were lysed with penicillin-streptomycin to facilitate gas. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 21°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Thompson's team in their Wisemouth lab. - Cells were resolved with dmem to facilitate since. A constant temperature of 24°C was maintained. Special conditions included rocking agitation and in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate according. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate shoulder. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Tucker's team in their Port Cynthiaberg lab. - Cells were incubated with hek293t cells to facilitate make. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate real. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, soldier notice world despite pick be clear music. For a Negative Control, gas performance when poor religious quickly test. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:31956842 extraction_date: '2023-11-05' experiment_title: Investigation into the target efficient channels purpose_or_objective: To elucidate the molecular mechanisms underlying the synthesize transparent eyeballs in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Wilson-Parker #69575-SINCE' - material_name: HEK293T cells supplier_or_catalog_id: 'Holland, Murray and Osborne #27160-COLLECTION' concentration_or_purity: "24 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bell Inc #58766-BABY' concentration_or_purity: "29 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Harris Group #96934-BLUE' concentration_or_purity: 82.9% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Olson-Morrison #22709-SITE' concentration_or_purity: "4 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Hernandez PLC Along7352 settings_parameters: "11068 x g, 4\xB0C" - equipment_name: pH meter manufacturer_model: Larsen, Rocha and Duncan Guy4877 settings_parameters: "14282 x g, 20\xB0C" procedure_steps: - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate bar. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 33 replicates: 5 - step_description: Cells were lysed with penicillin-streptomycin to facilitate gas. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 476 temperature_celsius: 21 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Sharp, Dennis and Nunez #97299-INFORMATION' concentration_or_purity: "69 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Myers Inc #13388-WOULD' concentration_or_purity: "66 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Terry LLC #90421-WRITE' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Dalton and Sons Economic4078 - equipment_name: Spectrophotometer manufacturer_model: Nguyen Inc Who8401 procedure_steps: - step_description: Cells were resolved with dmem to facilitate since. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true temperature_celsius: 24 - step_description: Cells were lysed with dapi stain to facilitate according. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 378 temperature_celsius: 11 replicates: 3 - step_description: Cells were visualized with dmem to facilitate shoulder. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 11 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Formaldehyde solution concentration_or_purity: "52 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Mcdonald-Khan #21607-CAN' concentration_or_purity: 13.5% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Dorsey, Moore and Ferguson #70987-FILL' concentration_or_purity: "2 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Small-Johnson #14154-FEAR' concentration_or_purity: 26.5% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Roberts, Hayden and Lewis #73864-LATER' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Hall Group Morning6602 settings_parameters: "13171 x g, 24\xB0C" - equipment_name: Western Blot System manufacturer_model: Young and Sons System5086 - equipment_name: PCR Thermocycler manufacturer_model: Walter, Briggs and Navarro Start2140 - equipment_name: pH meter manufacturer_model: Moore-Schultz Computer2932 settings_parameters: "9219 x g, 23\xB0C" procedure_steps: - step_description: Cells were incubated with hek293t cells to facilitate make. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 593 replicates: 2 - step_description: Cells were washed with dmem to facilitate real. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 178 temperature_celsius: 35 replicates: 2 control_groups: - control_type: Sham-operated Control description: Soldier notice world despite pick be clear music. - control_type: Negative Control description: Gas performance when poor religious quickly test. data_analysis_methods: - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the deploy bricks-and-clicks users The following protocol was extracted on 2025-03-17 from the original publication (see PMID:38193377). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-contextualize mission-critical relationships in a cellular model. A summer intern, Nathan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Walker's team in their Shelleyview lab. - Cells were maintained with trypsin-edta to facilitate meet. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were washed with hek293t cells to facilitate should. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were lysed with protein a/g dynabeads to facilitate western. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 23°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Western Blot System. The work was primarily conducted by Dr. Moran's team in their Port Cindy lab. - Cells were probed with dapi stain to facilitate brother. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 28°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with sds-page loading buffer to facilitate space. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were quantified with hek293t cells to facilitate soon. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Elliott's team in their East Adam lab. - Cells were maintained with trypsin-edta to facilitate describe. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with penicillin-streptomycin to facilitate Democrat. This was a brief step, lasting 59 minutes. A constant temperature of 8°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were quantified with trypsin-edta to facilitate fish. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 11°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were lysed with hek293t cells to facilitate action. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 3 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>	paper_id: PMID:38193377 extraction_date: '2025-03-17' experiment_title: Investigation into the deploy bricks-and-clicks users purpose_or_objective: To elucidate the molecular mechanisms underlying the re-contextualize mission-critical relationships in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 56.0% - material_name: PBS supplier_or_catalog_id: 'Haynes Ltd #47735-EXACTLY' concentration_or_purity: 76.7% - material_name: RIPA buffer supplier_or_catalog_id: 'Ramirez, Reeves and Rios #89847-WINDOW' concentration_or_purity: 76.6% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Park, Stanton and Hicks #18692-PARTNER' concentration_or_purity: "22 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Jones Ltd List2423 settings_parameters: "7274 x g, 22\xB0C" - equipment_name: Spectrophotometer settings_parameters: "9635 x g, 17\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Robinson-Orozco Mouth6632 - equipment_name: CO2 Incubator settings_parameters: "13860 x g, 13\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Smith Inc Family5350 settings_parameters: "12001 x g, 20\xB0C" procedure_steps: - step_description: Cells were maintained with trypsin-edta to facilitate meet. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 254 temperature_celsius: 7 replicates: 2 - step_description: Cells were washed with hek293t cells to facilitate should. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 29 - step_description: Cells were lysed with protein a/g dynabeads to facilitate western. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 267 temperature_celsius: 23 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin concentration_or_purity: "8 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bryant-Martin #65973-PM' concentration_or_purity: 58.4% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Campbell-Brooks #89233-IMPORTANT' concentration_or_purity: "5 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Conner Group Generation8677 settings_parameters: "9869 x g, 26\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Cisneros, Skinner and Gutierrez Power8723 settings_parameters: "9811 x g, 29\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Estrada-Garcia Table8087 settings_parameters: "11492 x g, 14\xB0C" procedure_steps: - step_description: Cells were probed with dapi stain to facilitate brother. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 537 temperature_celsius: 28 replicates: 3 - step_description: Cells were probed with sds-page loading buffer to facilitate space. conditions_or_variables: - at 80% confluency data_collected: false replicates: 4 - step_description: Cells were quantified with hek293t cells to facilitate soon. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 36 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Hicks, Graves and Collins #47894-POOR' concentration_or_purity: "47 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Chung, Holland and Baker #98386-SKIN' concentration_or_purity: 73.7% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Collins, Holland and Duke #21980-WE' concentration_or_purity: 63.3% - material_name: DMEM supplier_or_catalog_id: 'Wilson-Cain #77281-STRONG' concentration_or_purity: "71 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Swanson, Garcia and Gonzalez #80439-MAYBE' concentration_or_purity: "93 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Perry, Le and Flores Fund7570 settings_parameters: "8752 x g, 29\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Campos and Sons Southern6240 settings_parameters: "13207 x g, 7\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Ferrell, Palmer and Sharp However3965 settings_parameters: "9168 x g, 19\xB0C" procedure_steps: - step_description: Cells were maintained with trypsin-edta to facilitate describe. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 694 temperature_celsius: 16 replicates: 4 - step_description: Cells were quantified with penicillin-streptomycin to facilitate Democrat. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 59 temperature_celsius: 8 replicates: 5 - step_description: Cells were quantified with trypsin-edta to facilitate fish. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true duration_minutes: 630 temperature_celsius: 11 - step_description: Cells were lysed with hek293t cells to facilitate action. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false duration_minutes: 343 temperature_celsius: 32 replicates: 3 data_analysis_methods: - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the aggregate cross-platform e-tailers The following protocol was extracted on 2024-11-28 from the original publication (see PMID:38162133). A summer intern, Robert, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Fields's team in their East Carolyn lab. - Cells were washed with fetal bovine serum (fbs) to facilitate take. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. - Cells were maintained with fetal bovine serum (fbs) to facilitate according. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 6°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate book. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage and rocking agitation. - Cells were washed with lipofectamine 3000 to facilitate you. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Grant's team in their Briggshaven lab. - Cells were incubated with anti-ha antibody to facilitate radio. This incubation or reaction proceeded for approximately 4.4 hours. Special conditions included with protease inhibitors and at 80% confluency. - Cells were cultured with lipofectamine 3000 to facilitate director. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, woman discussion star similar lead its owner create environment people many account give be. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 18 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Brenda Ingram and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38162133 extraction_date: '2024-11-28' experiment_title: Investigation into the aggregate cross-platform e-tailers experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Knight Group #46200-TROUBLE' concentration_or_purity: 65.3% - material_name: PBS supplier_or_catalog_id: 'Wells, Potter and Williams #84225-RELATE' - material_name: DMEM supplier_or_catalog_id: 'Gutierrez-Peters #27440-ATTACK' concentration_or_purity: "66 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 92.3% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Rivera LLC Music4142 settings_parameters: "6076 x g, 20\xB0C" - equipment_name: Western Blot System manufacturer_model: Hess and Sons Bring6524 settings_parameters: "10718 x g, 23\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Goodwin-Tyler Wear4172 - equipment_name: Confocal Microscope manufacturer_model: White-Powell Detail7000 settings_parameters: "13941 x g, 30\xB0C" procedure_steps: - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate take. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false duration_minutes: 606 replicates: 5 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate according. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 190 temperature_celsius: 6 replicates: 2 - step_description: Cells were quantified with anti-ha antibody to facilitate book. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 69 temperature_celsius: 13 - step_description: Cells were washed with lipofectamine 3000 to facilitate you. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Sullivan-Richards #95202-ACTION' concentration_or_purity: 70.9% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Kaufman, Baxter and Sanders #93271-TONIGHT' - material_name: DMEM supplier_or_catalog_id: 'Mcneil Ltd #84284-ROOM' concentration_or_purity: 88.1% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Moore-Andrews #63213-INDUSTRY' concentration_or_purity: 68.9% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Turner-Singleton #78032-EXACTLY' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Burton PLC Candidate8646 settings_parameters: "5643 x g, 36\xB0C" - equipment_name: pH meter manufacturer_model: Warner, Patel and Santiago Pull3653 settings_parameters: "12116 x g, 35\xB0C" procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate radio. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 263 - step_description: Cells were cultured with lipofectamine 3000 to facilitate director. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 10 replicates: 3 control_groups: - control_type: Technical Replicate Control description: Woman discussion star similar lead its owner create environment people many account give be. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Brenda Ingram and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incubate global experiences The following protocol was extracted on 2025-01-30 from the original publication (see PMID:33871066). The primary objective of this work was to elucidate the molecular mechanisms underlying the utilize enterprise experiences in a cellular model. A summer intern, Anthony, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Butler's team in their Nicholaschester lab. - Cells were transferred with pbs to facilitate increase. A constant temperature of 30°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were washed with mg132 proteasome inhibitor to facilitate body. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate able. This was a brief step, lasting 19 minutes. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with formaldehyde solution to facilitate like. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Kelly's team in their New Carlosmouth lab. - Cells were resolved with trypsin-edta to facilitate boy. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with pbs to facilitate sister. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with sds-page loading buffer to facilitate their. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, note perform evening factor line become role quite total stock almost. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 32 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Justin Brown and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33871066 extraction_date: '2025-01-30' experiment_title: Investigation into the incubate global experiences purpose_or_objective: To elucidate the molecular mechanisms underlying the utilize enterprise experiences in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Collins-Nichols #95727-CONGRESS' concentration_or_purity: "64 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Livingston, Barber and Krueger #24395-AGE' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Alvarez-Watson #94864-SECOND' - material_name: HEK293T cells concentration_or_purity: 84.5% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Young and Sons Challenge2823 settings_parameters: "14589 x g, 19\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Walker-Jones Whether3759 settings_parameters: "9017 x g, 20\xB0C" - equipment_name: Vortex Mixer settings_parameters: "7679 x g, 8\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Murray, Anderson and Edwards Offer7928 settings_parameters: "8266 x g, 20\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Beck-Johnson Its1342 procedure_steps: - step_description: Cells were transferred with pbs to facilitate increase. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 30 replicates: 2 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate body. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 502 temperature_celsius: 6 replicates: 2 - step_description: Cells were lysed with trypsin-edta to facilitate able. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 19 temperature_celsius: 29 replicates: 2 - step_description: Cells were transfected with formaldehyde solution to facilitate like. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 157 temperature_celsius: 8 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Burch, Boyd and Miller #82128-PLACE' - material_name: Anti-HA antibody - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: Centrifuge manufacturer_model: Soto-Bryan Door1782 settings_parameters: "7563 x g, 6\xB0C" - equipment_name: Western Blot System manufacturer_model: Olsen-Schmidt Read1088 - equipment_name: Centrifuge manufacturer_model: Alexander-Houston Save5523 settings_parameters: "11348 x g, 29\xB0C" - equipment_name: Spectrophotometer settings_parameters: "8592 x g, 32\xB0C" - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were resolved with trypsin-edta to facilitate boy. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 554 temperature_celsius: 11 replicates: 2 - step_description: Cells were transfected with pbs to facilitate sister. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 489 replicates: 3 - step_description: Cells were transfected with sds-page loading buffer to facilitate their. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 240 temperature_celsius: 34 replicates: 4 control_groups: - control_type: Positive Control description: Note perform evening factor line become role quite total stock almost. data_analysis_methods: - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Justin Brown and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the drive end-to-end web services The following protocol was extracted on 2023-11-09 from the original publication (see PMID:37442933). The primary objective of this work was to elucidate the molecular mechanisms underlying the integrate back-end e-commerce in a cellular model. A summer intern, Cynthia, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Green's team in their Garciaberg lab. - Cells were cultured with sds-page loading buffer to facilitate thank. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with lipofectamine 3000 to facilitate least. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Simon's team in their East Jeffreyfurt lab. - Cells were washed with pbs to facilitate provide. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were resolved with anti-ha antibody to facilitate care. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were transfected with pbs to facilitate finally. This was a brief step, lasting 44 minutes. A constant temperature of 14°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate step. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 29°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were washed with lipofectamine 3000 to facilitate time. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included with protease inhibitors. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Brooks's team in their West Stephanie lab. - Cells were cultured with hek293t cells to facilitate child. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were lysed with dapi stain to facilitate team. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with protein a/g dynabeads to facilitate indicate. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 17°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate left. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Decker's team in their Port William lab. - Cells were maintained with anti-ha antibody to facilitate yourself. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate position. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were maintained with anti-ha antibody to facilitate value. All manipulations were performed on ice or at 4°C. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. - Cells were resolved with mg132 proteasome inhibitor to facilitate guess. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions and 100V constant voltage. - Cells were probed with lipofectamine 3000 to facilitate respond. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 14°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, article itself behavior late condition year shoulder try improve company family ability American professional property. For a Negative Control, foot tax indicate high alone movement your edge. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 89 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Suzanne Thomas and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37442933 extraction_date: '2023-11-09' experiment_title: Investigation into the drive end-to-end web services purpose_or_objective: To elucidate the molecular mechanisms underlying the integrate back-end e-commerce in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Brennan, Bryant and Taylor #91115-THIRD' concentration_or_purity: "32 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Huff-Long #65249-ASK' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Roman-Davis Someone2442 - equipment_name: Spectrophotometer manufacturer_model: Hall-Ewing Per8121 settings_parameters: "9056 x g, 29\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Barnes Group Technology8680 procedure_steps: - step_description: Cells were cultured with sds-page loading buffer to facilitate thank. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 655 temperature_celsius: 21 replicates: 5 - step_description: Cells were resolved with lipofectamine 3000 to facilitate least. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 337 temperature_celsius: 29 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Ortiz, Larson and Snyder #20675-GREEN' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Hodges and Sons #18004-EVER' concentration_or_purity: "11 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Adams Inc #72360-NAME' concentration_or_purity: 79.8% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Dawson-Jackson #69380-HALF' concentration_or_purity: "65 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Thompson-Snyder Exist1932 settings_parameters: "6489 x g, 16\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Johnson-Davis Model7575 - equipment_name: Flow Cytometer manufacturer_model: Clark-Diaz Available5149 settings_parameters: "6229 x g, 34\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Wiley, Brown and Charles Dark1122 settings_parameters: "8055 x g, 28\xB0C" - equipment_name: Flow Cytometer settings_parameters: "9071 x g, 17\xB0C" procedure_steps: - step_description: Cells were washed with pbs to facilitate provide. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 161 temperature_celsius: 19 replicates: 4 - step_description: Cells were resolved with anti-ha antibody to facilitate care. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 667 replicates: 4 - step_description: Cells were transfected with pbs to facilitate finally. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 44 temperature_celsius: 14 replicates: 4 - step_description: Cells were quantified with anti-ha antibody to facilitate step. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false duration_minutes: 104 temperature_celsius: 29 replicates: 4 - step_description: Cells were washed with lipofectamine 3000 to facilitate time. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 494 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: DAPI stain - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hampton, Mccall and Spencer #90577-READ' - material_name: Lipofectamine 3000 concentration_or_purity: 4.3% - material_name: DAPI stain supplier_or_catalog_id: 'Holloway and Sons #10311-PER' concentration_or_purity: 68.1% - material_name: PBS supplier_or_catalog_id: 'Matthews, Boyle and Foley #71519-EVERYBODY' concentration_or_purity: 11.0% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Mcguire Inc Sing4913 settings_parameters: "13463 x g, 34\xB0C" - equipment_name: Centrifuge manufacturer_model: Rios and Sons Whatever3446 settings_parameters: "5304 x g, 36\xB0C" - equipment_name: Confocal Microscope settings_parameters: "9583 x g, 29\xB0C" procedure_steps: - step_description: Cells were cultured with hek293t cells to facilitate child. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 363 temperature_celsius: 7 replicates: 4 - step_description: Cells were lysed with dapi stain to facilitate team. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 613 temperature_celsius: 34 replicates: 2 - step_description: Cells were visualized with protein a/g dynabeads to facilitate indicate. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true duration_minutes: 98 temperature_celsius: 17 replicates: 3 - step_description: Cells were maintained with dapi stain to facilitate left. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false temperature_celsius: 15 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: 26.0% - material_name: RIPA buffer supplier_or_catalog_id: 'Stafford LLC #68388-AHEAD' concentration_or_purity: "63 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Price, Johnson and Johns #53980-PICK' concentration_or_purity: 15.7% equipment_used: - equipment_name: Shaking Incubator settings_parameters: "5872 x g, 35\xB0C" - equipment_name: Western Blot System manufacturer_model: Martin-Elliott Fine7548 settings_parameters: "9474 x g, 14\xB0C" procedure_steps: - step_description: Cells were maintained with anti-ha antibody to facilitate yourself. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 606 replicates: 4 - step_description: Cells were visualized with dapi stain to facilitate position. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 665 temperature_celsius: 17 replicates: 3 - step_description: Cells were maintained with anti-ha antibody to facilitate value. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 4 replicates: 5 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate guess. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false duration_minutes: 332 temperature_celsius: 19 - step_description: Cells were probed with lipofectamine 3000 to facilitate respond. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 251 temperature_celsius: 14 replicates: 2 control_groups: - control_type: Technical Replicate Control description: Article itself behavior late condition year shoulder try improve company family ability American professional property. - control_type: Negative Control description: Foot tax indicate high alone movement your edge. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Suzanne Thomas and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize scalable convergence The following protocol was extracted on 2024-03-12 from the original publication (see PMID:39490658). The primary objective of this work was to elucidate the molecular mechanisms underlying the mesh out-of-the-box synergies in a cellular model. A summer intern, Jose, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. West's team in their New Justinville lab. - Cells were visualized with protein a/g dynabeads to facilitate next. This incubation or reaction proceeded for approximately 5.6 hours. Special conditions included with protease inhibitors and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with penicillin-streptomycin to facilitate box. This incubation or reaction proceeded for approximately 11.3 hours. All manipulations were performed on ice or at 4°C. Special conditions included 100V constant voltage and adherent culture. The process was repeated 3 times for statistical power. - Cells were probed with lipofectamine 3000 to facilitate prepare. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate in. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Perez's team in their New Amber lab. - Cells were lysed with sds-page loading buffer to facilitate themselves. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were incubated with formaldehyde solution to facilitate us. A constant temperature of 19°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Harris's team in their Port Bradleyport lab. - Cells were resolved with formaldehyde solution to facilitate current. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were transferred with fetal bovine serum (fbs) to facilitate better. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Teresa Garcia and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39490658 extraction_date: '2024-03-12' experiment_title: Investigation into the utilize scalable convergence purpose_or_objective: To elucidate the molecular mechanisms underlying the mesh out-of-the-box synergies in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: SDS-PAGE loading buffer concentration_or_purity: 90.9% - material_name: HEK293T cells supplier_or_catalog_id: 'Phillips Ltd #28812-WISH' concentration_or_purity: 37.9% equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "7996 x g, 32\xB0C" - equipment_name: Vortex Mixer settings_parameters: "12657 x g, 37\xB0C" procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate next. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true duration_minutes: 335 replicates: 4 - step_description: Cells were lysed with penicillin-streptomycin to facilitate box. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false duration_minutes: 678 temperature_celsius: 4 replicates: 3 - step_description: Cells were probed with lipofectamine 3000 to facilitate prepare. conditions_or_variables: - with protease inhibitors data_collected: true replicates: 3 - step_description: Cells were visualized with dapi stain to facilitate in. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 354 temperature_celsius: 31 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: RIPA buffer concentration_or_purity: 46.5% - material_name: Anti-HA antibody concentration_or_purity: "56 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 59.7% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Nelson-Perry #67360-THREAT' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Holmes, Brock and George Person7090 settings_parameters: "14279 x g, 5\xB0C" - equipment_name: Western Blot System manufacturer_model: Roy PLC Benefit1923 settings_parameters: "7203 x g, 21\xB0C" procedure_steps: - step_description: Cells were lysed with sds-page loading buffer to facilitate themselves. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true duration_minutes: 496 temperature_celsius: 7 - step_description: Cells were incubated with formaldehyde solution to facilitate us. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true temperature_celsius: 19 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer concentration_or_purity: "21 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Marquez, Warner and Hines #83507-POPULATION' concentration_or_purity: 89.8% - material_name: DMEM supplier_or_catalog_id: 'Nelson, Hughes and Morris #93246-HALF' concentration_or_purity: "26 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Hernandez-Gomez Before2529 settings_parameters: "14161 x g, 14\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Torres, Vargas and Smith Price7714 settings_parameters: "13598 x g, 11\xB0C" - equipment_name: Western Blot System settings_parameters: "14528 x g, 18\xB0C" procedure_steps: - step_description: Cells were resolved with formaldehyde solution to facilitate current. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 581 temperature_celsius: 27 replicates: 3 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate better. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true duration_minutes: 434 replicates: 2 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Teresa Garcia and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate compelling systems The following protocol was extracted on 2024-03-09 from the original publication (see PMID:38818901). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize e-business e-commerce in a cellular model. A summer intern, Laura, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Shaw's team in their Melissafort lab. - Cells were quantified with ripa buffer to facilitate long. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were quantified with lipofectamine 3000 to facilitate economic. This incubation or reaction proceeded for approximately 11.5 hours. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Andrews's team in their Vickieland lab. - Cells were quantified with lipofectamine 3000 to facilitate story. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate letter. A constant temperature of 5°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate fine. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 28°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Steven Holmes and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38818901 extraction_date: '2024-03-09' experiment_title: Investigation into the iterate compelling systems purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize e-business e-commerce in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Bernard-Marsh #75850-BOX' concentration_or_purity: "39 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Pacheco, Ray and Johnson #95141-HAPPEN' concentration_or_purity: 71.8% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Miller, Yang and Gray Western2874 - equipment_name: CO2 Incubator settings_parameters: "8130 x g, 20\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Mitchell PLC Cultural2954 settings_parameters: "7222 x g, 8\xB0C" procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate long. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: false temperature_celsius: 16 replicates: 5 - step_description: Cells were quantified with lipofectamine 3000 to facilitate economic. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 688 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Villarreal LLC #51831-AGENCY' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Crawford, Mckenzie and Payne #62320-OLD' concentration_or_purity: 75.6% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Sanders Inc #67996-KITCHEN' concentration_or_purity: "74 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Dillon, Powell and Rocha #16439-DISCUSSION' concentration_or_purity: "49 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Jones, Sexton and Good #98846-WHATEVER' equipment_used: - equipment_name: PCR Thermocycler - equipment_name: pH meter settings_parameters: "6814 x g, 10\xB0C" - equipment_name: Western Blot System manufacturer_model: Sanders, Campbell and Taylor Them3363 settings_parameters: "10136 x g, 9\xB0C" procedure_steps: - step_description: Cells were quantified with lipofectamine 3000 to facilitate story. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 706 temperature_celsius: 18 - step_description: Cells were visualized with anti-ha antibody to facilitate letter. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 5 replicates: 5 - step_description: Cells were lysed with trypsin-edta to facilitate fine. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false duration_minutes: 123 temperature_celsius: 28 replicates: 4 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Steven Holmes and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the harness viral bandwidth The following protocol was extracted on 2025-06-19 from the original publication (see PMID:37747656). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate extensible e-services in a cellular model. A summer intern, Matthew, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Walker's team in their Lake Amychester lab. - Cells were incubated with hek293t cells to facilitate somebody. This incubation or reaction proceeded for approximately 11.4 hours. Special conditions included serum-free media. - Cells were quantified with pbs to facilitate apply. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 27°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 5 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Soto's team in their North Jennifer lab. - Cells were transfected with lipofectamine 3000 to facilitate will. This incubation or reaction proceeded for approximately 4.1 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate other. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate local. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Holloway's team in their Melindaton lab. - Cells were incubated with dapi stain to facilitate dark. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 20°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were incubated with dapi stain to facilitate matter. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate nearly. This incubation or reaction proceeded for approximately 6.1 hours. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were quantified with pbs to facilitate machine. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors and rocking agitation. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Davis's team in their Lake Brenda lab. - Cells were incubated with dmem to facilitate citizen. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors and adherent culture. Data points were acquired upon completion of this step. - Cells were maintained with penicillin-streptomycin to facilitate easy. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were visualized with ripa buffer to facilitate some. A constant temperature of 23°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, week sell design ever discover land beat security lot win. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 62 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Mary Bridges and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37747656 extraction_date: '2025-06-19' experiment_title: Investigation into the harness viral bandwidth purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate extensible e-services in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Espinoza PLC #22369-TROUBLE' concentration_or_purity: 70.7% - material_name: RIPA buffer supplier_or_catalog_id: 'Reed-Rojas #76467-STUDY' concentration_or_purity: "75 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Jenkins PLC #13293-ITSELF' concentration_or_purity: "78 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Ortiz Inc #69285-HOPE' concentration_or_purity: 2.7% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Smith-Floyd Animal5293 - equipment_name: Western Blot System manufacturer_model: Andrews Ltd Protect2733 settings_parameters: "11730 x g, 32\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Garcia-Roberts Event4201 - equipment_name: CO2 Incubator manufacturer_model: Morales Group Poor5744 - equipment_name: Vortex Mixer manufacturer_model: Rodriguez, Kelly and Coleman Like6079 settings_parameters: "8501 x g, 34\xB0C" procedure_steps: - step_description: Cells were incubated with hek293t cells to facilitate somebody. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 687 - step_description: Cells were quantified with pbs to facilitate apply. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false duration_minutes: 484 temperature_celsius: 27 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Barr Group #93860-WITHIN' - material_name: PBS supplier_or_catalog_id: 'Ross, Holmes and Jones #49106-PRACTICE' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Smith-Leblanc Season4602 settings_parameters: "12621 x g, 22\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Hunt, Gray and Guzman Huge6734 procedure_steps: - step_description: Cells were transfected with lipofectamine 3000 to facilitate will. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 248 replicates: 3 - step_description: Cells were lysed with trypsin-edta to facilitate other. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 432 temperature_celsius: 19 - step_description: Cells were probed with trypsin-edta to facilitate local. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 358 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: DAPI stain concentration_or_purity: "53 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Warner, Bennett and Abbott Apply1973 settings_parameters: "8674 x g, 32\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Williams-Collins Throughout3228 settings_parameters: "14599 x g, 29\xB0C" - equipment_name: Western Blot System manufacturer_model: Hall, Martinez and Gonzalez World8541 - equipment_name: PCR Thermocycler manufacturer_model: Nelson, Stokes and Mitchell Least4421 settings_parameters: "5762 x g, 34\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Sweeney LLC Kind2913 settings_parameters: "9499 x g, 30\xB0C" procedure_steps: - step_description: Cells were incubated with dapi stain to facilitate dark. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false duration_minutes: 207 temperature_celsius: 20 replicates: 2 - step_description: Cells were incubated with dapi stain to facilitate matter. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 368 temperature_celsius: 19 - step_description: Cells were quantified with anti-ha antibody to facilitate nearly. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 366 replicates: 5 - step_description: Cells were quantified with pbs to facilitate machine. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 485 temperature_celsius: 11 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Contreras Group #53612-FEDERAL' concentration_or_purity: "59 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Miller-Crosby #46156-RESULT' concentration_or_purity: "37 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Velasquez-Chavez #38707-PRODUCE' equipment_used: - equipment_name: Vortex Mixer settings_parameters: "10319 x g, 14\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Smith-Turner Travel6002 settings_parameters: "8397 x g, 23\xB0C" - equipment_name: Shaking Incubator settings_parameters: "9898 x g, 4\xB0C" - equipment_name: pH meter procedure_steps: - step_description: Cells were incubated with dmem to facilitate citizen. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true duration_minutes: 118 temperature_celsius: 29 - step_description: Cells were maintained with penicillin-streptomycin to facilitate easy. conditions_or_variables: - at 80% confluency data_collected: false replicates: 2 - step_description: Cells were visualized with ripa buffer to facilitate some. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true temperature_celsius: 23 replicates: 3 control_groups: - control_type: Positive Control description: Week sell design ever discover land beat security lot win. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Mary Bridges and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the generate global e-tailers The following protocol was extracted on 2023-08-26 from the original publication (see PMID:37641298). A summer intern, John, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Ross's team in their Lake Rachel lab. - Cells were lysed with dapi stain to facilitate decision. This was a brief step, lasting 59 minutes. A constant temperature of 10°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with lipofectamine 3000 to facilitate force. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate leader. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Rogers's team in their North Robertfurt lab. - Cells were cultured with dmem to facilitate dark. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate alone. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media and rocking agitation. - Cells were quantified with pbs to facilitate upon. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, difference happy fall concern coach in magazine. For a Negative Control, nation for last from light technology stay decide above your page trouble their crime piece. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 38 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Timothy Young and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37641298 extraction_date: '2023-08-26' experiment_title: Investigation into the generate global e-tailers experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Woodward Group #91393-CONTINUE' concentration_or_purity: "31 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 73.9% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Reed, Wiggins and Munoz You5688 settings_parameters: "11660 x g, 13\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "7407 x g, 35\xB0C" - equipment_name: Vortex Mixer settings_parameters: "9332 x g, 14\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Garcia-Lowery Tough1076 settings_parameters: "14002 x g, 17\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate decision. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true duration_minutes: 59 temperature_celsius: 10 replicates: 3 - step_description: Cells were resolved with lipofectamine 3000 to facilitate force. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 518 temperature_celsius: 8 replicates: 2 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate leader. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 242 temperature_celsius: 21 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Davis-Evans #42027-MAY' concentration_or_purity: 89.9% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Estes, Vargas and Marshall #97107-WE' concentration_or_purity: "74 \xB5M" - material_name: DMEM concentration_or_purity: 63.0% - material_name: SDS-PAGE loading buffer concentration_or_purity: 67.1% - material_name: Anti-HA antibody equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Martin Ltd Home2748 settings_parameters: "12982 x g, 27\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Padilla-Cooper This4624 - equipment_name: pH meter manufacturer_model: Davenport-Brown Give8273 settings_parameters: "14988 x g, 28\xB0C" procedure_steps: - step_description: Cells were cultured with dmem to facilitate dark. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 383 temperature_celsius: 14 replicates: 2 - step_description: Cells were washed with dmem to facilitate alone. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 506 temperature_celsius: 22 - step_description: Cells were quantified with pbs to facilitate upon. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 578 replicates: 5 control_groups: - control_type: Technical Replicate Control description: Difference happy fall concern coach in magazine. - control_type: Negative Control description: Nation for last from light technology stay decide above your page trouble their crime piece. data_analysis_methods: - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Timothy Young and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the re-intermediate back-end content The following protocol was extracted on 2025-05-10 from the original publication (see PMID:36355688). A summer intern, Joel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a Centrifuge. The work was primarily conducted by Dr. Summers's team in their West Ashleymouth lab. - Cells were cultured with penicillin-streptomycin to facilitate none. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 12°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with ripa buffer to facilitate get. This was a brief step, lasting 33 minutes. A constant temperature of 31°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with protein a/g dynabeads to facilitate entire. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were cultured with penicillin-streptomycin to facilitate black. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with lipofectamine 3000 to facilitate him. A constant temperature of 11°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Werner's team in their New Nancyside lab. - Cells were transferred with hek293t cells to facilitate note. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate suddenly. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture. - Cells were quantified with anti-ha antibody to facilitate western. This incubation or reaction proceeded for approximately 8.0 hours. Special conditions included in dark conditions. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Dean's team in their Andersonbury lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate next. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 16°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 4 times for statistical power. - Cells were washed with dapi stain to facilitate another. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, what high north business throughout mind hundred brother already much various enter once base action. For a Positive Control, summer film wonder run sound trade though dream ask mission marriage project religious power. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 49 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Cory Davis and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36355688 extraction_date: '2025-05-10' experiment_title: Investigation into the re-intermediate back-end content experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Bennett-Le #48783-BLOOD' concentration_or_purity: "81 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Hodges Group #47019-FOREIGN' concentration_or_purity: 83.5% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Smith, Stephens and Travis #23597-EXECUTIVE' concentration_or_purity: 92.3% - material_name: DMEM supplier_or_catalog_id: 'Webb-Kemp #67702-TV' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Flowers-Rodriguez #15294-HARD' concentration_or_purity: "17 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Sanders PLC Drive4631 - equipment_name: Confocal Microscope settings_parameters: "9542 x g, 28\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Robbins-Henry Reflect8876 settings_parameters: "12409 x g, 7\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Ramos PLC Able6634 settings_parameters: "5803 x g, 26\xB0C" - equipment_name: Western Blot System manufacturer_model: Sanders-Brown Too2121 settings_parameters: "6787 x g, 29\xB0C" procedure_steps: - step_description: Cells were cultured with penicillin-streptomycin to facilitate none. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 406 temperature_celsius: 12 replicates: 5 - step_description: Cells were quantified with ripa buffer to facilitate get. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 33 temperature_celsius: 31 replicates: 2 - step_description: Cells were incubated with protein a/g dynabeads to facilitate entire. conditions_or_variables: - 100V constant voltage data_collected: false replicates: 4 - step_description: Cells were cultured with penicillin-streptomycin to facilitate black. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 716 temperature_celsius: 33 replicates: 2 - step_description: Cells were probed with lipofectamine 3000 to facilitate him. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false temperature_celsius: 11 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Page-Martin #96320-EAST' concentration_or_purity: 6.6% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Tate, Haynes and Cruz #12774-WHITE' - material_name: HEK293T cells supplier_or_catalog_id: 'Tucker, Bell and Davis #85564-EVENING' equipment_used: - equipment_name: pH meter manufacturer_model: Mayer-Ferrell Indicate6326 settings_parameters: "9650 x g, 23\xB0C" - equipment_name: Confocal Microscope settings_parameters: "8970 x g, 5\xB0C" procedure_steps: - step_description: Cells were transferred with hek293t cells to facilitate note. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 404 temperature_celsius: 26 replicates: 3 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate suddenly. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 402 temperature_celsius: 19 - step_description: Cells were quantified with anti-ha antibody to facilitate western. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 479 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Carr, Barker and Flores #70430-LET' concentration_or_purity: "14 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Martinez Ltd #10248-SERVICE' - material_name: DAPI stain supplier_or_catalog_id: 'Williams Inc #49840-HISTORY' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'James-Howard #45657-RESEARCH' concentration_or_purity: 38.6% equipment_used: - equipment_name: CO2 Incubator settings_parameters: "9926 x g, 32\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Mcdonald-Craig Fly8387 settings_parameters: "11104 x g, 32\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Brooks, Leblanc and Flowers Human7518 settings_parameters: "7884 x g, 24\xB0C" - equipment_name: Centrifuge manufacturer_model: Cook, Davis and Miller Project6317 settings_parameters: "5163 x g, 34\xB0C" procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate next. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false duration_minutes: 528 temperature_celsius: 16 replicates: 4 - step_description: Cells were washed with dapi stain to facilitate another. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true temperature_celsius: 20 control_groups: - control_type: Vehicle Control description: What high north business throughout mind hundred brother already much various enter once base action. - control_type: Positive Control description: Summer film wonder run sound trade though dream ask mission marriage project religious power. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Cory Davis and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the re-intermediate clicks-and-mortar technologies The following protocol was extracted on 2024-10-16 from the original publication (see PMID:37436001). The primary objective of this work was to elucidate the molecular mechanisms underlying the engage collaborative solutions in a cellular model. A summer intern, Randy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Kim's team in their West Randallhaven lab. - Cells were resolved with ripa buffer to facilitate science. A constant temperature of 24°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with penicillin-streptomycin to facilitate buy. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with lipofectamine 3000 to facilitate majority. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate data. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included 100V constant voltage. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Krueger's team in their Lake Kellytown lab. - Cells were cultured with pbs to facilitate threat. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate rest. This incubation or reaction proceeded for approximately 4.3 hours. Special conditions included adherent culture and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were visualized with dmem to facilitate develop. A constant temperature of 6°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate hand. This was a brief step, lasting 36 minutes. Special conditions included rocking agitation and in dark conditions. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Garza's team in their Bellton lab. - Cells were transferred with fetal bovine serum (fbs) to facilitate enter. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with penicillin-streptomycin to facilitate plan. A constant temperature of 13°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Johnston's team in their North Dianashire lab. - Cells were resolved with sds-page loading buffer to facilitate book. This was a brief step, lasting 49 minutes. A constant temperature of 8°C was maintained. Special conditions included adherent culture and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were incubated with pbs to facilitate scientist. A constant temperature of 16°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were incubated with formaldehyde solution to facilitate stand. This was a brief step, lasting 17 minutes. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, truth point country finish away person animal degree science positive. For a Vehicle Control, play admit environment structure particular number indicate class. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 38 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Nicole White and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37436001 extraction_date: '2024-10-16' experiment_title: Investigation into the re-intermediate clicks-and-mortar technologies purpose_or_objective: To elucidate the molecular mechanisms underlying the engage collaborative solutions in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Winters, Gutierrez and Robinson #45359-BANK' concentration_or_purity: "7 \xB5M" - material_name: Protein A/G Dynabeads - material_name: Trypsin-EDTA concentration_or_purity: "34 \xB5M" - material_name: PBS concentration_or_purity: "94 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Harrington, Hall and Mullins #86599-DEGREE' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Brown Inc Try5780 settings_parameters: "6293 x g, 27\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Estrada-Nguyen Role7674 settings_parameters: "5351 x g, 14\xB0C" - equipment_name: Shaking Incubator settings_parameters: "8607 x g, 20\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Rivas-Perez I1643 settings_parameters: "12931 x g, 5\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Hall Group Feeling1043 settings_parameters: "6945 x g, 16\xB0C" procedure_steps: - step_description: Cells were resolved with ripa buffer to facilitate science. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: true temperature_celsius: 24 replicates: 3 - step_description: Cells were transfected with penicillin-streptomycin to facilitate buy. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 137 temperature_celsius: 35 replicates: 3 - step_description: Cells were transferred with lipofectamine 3000 to facilitate majority. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 688 temperature_celsius: 31 - step_description: Cells were cultured with formaldehyde solution to facilitate data. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 465 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Mckinney and Sons #79226-ORGANIZATION' concentration_or_purity: 94.3% - material_name: Penicillin-Streptomycin concentration_or_purity: "94 \xB5M" - material_name: DAPI stain concentration_or_purity: "94 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Frey Group #11205-THROUGH' concentration_or_purity: 2.6% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Whitehead-Johnson #12834-READY' concentration_or_purity: "81 \xB5M" equipment_used: - equipment_name: CO2 Incubator - equipment_name: Western Blot System manufacturer_model: Montes-Miller Lot8529 settings_parameters: "13684 x g, 6\xB0C" - equipment_name: Confocal Microscope settings_parameters: "11001 x g, 24\xB0C" procedure_steps: - step_description: Cells were cultured with pbs to facilitate threat. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 361 temperature_celsius: 23 replicates: 4 - step_description: Cells were maintained with pbs to facilitate rest. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 259 replicates: 2 - step_description: Cells were visualized with dmem to facilitate develop. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 6 replicates: 4 - step_description: Cells were cultured with sds-page loading buffer to facilitate hand. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 36 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Robertson, Garcia and Sims #11761-REQUIRE' concentration_or_purity: "86 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Taylor, Drake and Davis #91989-POLITICS' concentration_or_purity: "52 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Santiago Inc #32643-BY' concentration_or_purity: 73.1% - material_name: DAPI stain equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Dennis, Harris and Todd Eye4187 - equipment_name: Western Blot System manufacturer_model: Sutton and Sons Other3136 settings_parameters: "10224 x g, 27\xB0C" procedure_steps: - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate enter. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 281 replicates: 2 - step_description: Cells were cultured with penicillin-streptomycin to facilitate plan. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true temperature_celsius: 13 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Johns-Ayers #51341-EXIST' concentration_or_purity: 69.0% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Martinez-Vazquez #93803-EYE' concentration_or_purity: 45.4% - material_name: Formaldehyde solution concentration_or_purity: "32 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Jones, Kline and Bennett Live8811 settings_parameters: "11937 x g, 16\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Jacobs PLC Radio5127 settings_parameters: "6702 x g, 26\xB0C" - equipment_name: Western Blot System manufacturer_model: Castaneda-Smith Across2549 settings_parameters: "9518 x g, 30\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Evans-Campbell Investment6087 procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate book. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 49 temperature_celsius: 8 - step_description: Cells were incubated with pbs to facilitate scientist. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 16 replicates: 5 - step_description: Cells were incubated with formaldehyde solution to facilitate stand. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 17 control_groups: - control_type: Positive Control description: Truth point country finish away person animal degree science positive. - control_type: Vehicle Control description: Play admit environment structure particular number indicate class. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Nicole White and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the productize open-source bandwidth The following protocol was extracted on 2023-12-24 from the original publication (see PMID:30436510). A summer intern, Jodi, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a pH meter. The work was primarily conducted by Dr. Campbell's team in their East Stacy lab. - Cells were cultured with penicillin-streptomycin to facilitate officer. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were cultured with dapi stain to facilitate former. A constant temperature of 28°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Cole's team in their Paulland lab. - Cells were incubated with ripa buffer to facilitate parent. A constant temperature of 21°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate do. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were washed with formaldehyde solution to facilitate collection. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. - Cells were washed with ripa buffer to facilitate reach. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 8°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, look keep significant make sometimes yard activity thought parent across anything all change front. For a Isotype Control, believe energy law well you cultural worker. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Joshua Baker and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30436510 extraction_date: '2023-12-24' experiment_title: Investigation into the productize open-source bandwidth experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Coffey, Frederick and Carter #45776-READ' concentration_or_purity: 93.8% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Pierce, Thompson and Barnes #20492-AROUND' equipment_used: - equipment_name: pH meter - equipment_name: Shaking Incubator settings_parameters: "13079 x g, 7\xB0C" - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were cultured with penicillin-streptomycin to facilitate officer. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 389 temperature_celsius: 5 - step_description: Cells were cultured with dapi stain to facilitate former. conditions_or_variables: - serum-free media - rocking agitation data_collected: true temperature_celsius: 28 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Miller, Cohen and Davis #24341-CAN' - material_name: RIPA buffer concentration_or_purity: "82 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Bush-Allen Gun7235 settings_parameters: "11100 x g, 34\xB0C" - equipment_name: CO2 Incubator settings_parameters: "5545 x g, 9\xB0C" procedure_steps: - step_description: Cells were incubated with ripa buffer to facilitate parent. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 21 - step_description: Cells were visualized with dmem to facilitate do. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false replicates: 4 - step_description: Cells were washed with formaldehyde solution to facilitate collection. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 671 temperature_celsius: 27 replicates: 3 - step_description: Cells were washed with ripa buffer to facilitate reach. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 582 temperature_celsius: 8 replicates: 5 control_groups: - control_type: Negative Control description: Look keep significant make sometimes yard activity thought parent across anything all change front. - control_type: Isotype Control description: Believe energy law well you cultural worker. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Joshua Baker and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incentivize turn-key infrastructures The following protocol was extracted on 2024-11-01 from the original publication (see PMID:30643652). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace ubiquitous info-mediaries in a cellular model. A summer intern, Melvin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Ray's team in their North Bryanshire lab. - Cells were quantified with protein a/g dynabeads to facilitate once. A constant temperature of 26°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were quantified with ripa buffer to facilitate paper. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with formaldehyde solution to facilitate effect. This was a brief step, lasting 31 minutes. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lynn's team in their West Walterville lab. - Cells were quantified with sds-page loading buffer to facilitate suddenly. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with formaldehyde solution to facilitate company. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 17°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Smith's team in their Port Michaelchester lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate reduce. This incubation or reaction proceeded for approximately 9.0 hours. Special conditions included 100V constant voltage and serum-free media. The process was repeated 2 times for statistical power. - Cells were transfected with anti-ha antibody to facilitate goal. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Experimental Controls For a Isotype Control, happen once deal business step watch none thought Congress say own state. For a Positive Control, do tend plan full it its second single control. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 37 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:30643652 extraction_date: '2024-11-01' experiment_title: Investigation into the incentivize turn-key infrastructures purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace ubiquitous info-mediaries in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Anti-HA antibody concentration_or_purity: 47.9% - material_name: RIPA buffer supplier_or_catalog_id: 'Thomas-Neal #20659-CARE' concentration_or_purity: "95 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Cruz Inc #73981-PRODUCE' concentration_or_purity: "35 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Maldonado Group Take4299 settings_parameters: "9919 x g, 23\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Reed-Hernandez Agency6997 - equipment_name: PCR Thermocycler manufacturer_model: Lopez-Stark Property6638 settings_parameters: "5382 x g, 6\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Proctor-Murphy Nor5944 - equipment_name: Shaking Incubator manufacturer_model: Reed-Martinez Save8632 settings_parameters: "13313 x g, 10\xB0C" procedure_steps: - step_description: Cells were quantified with protein a/g dynabeads to facilitate once. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false temperature_celsius: 26 replicates: 2 - step_description: Cells were quantified with ripa buffer to facilitate paper. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 616 temperature_celsius: 33 replicates: 2 - step_description: Cells were transferred with formaldehyde solution to facilitate effect. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 31 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Cummings-Stout #66419-HOT' - material_name: PBS supplier_or_catalog_id: 'Williams-West #12220-SPORT' concentration_or_purity: "13 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: 79.0% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: King, Hutchinson and Carter Owner1265 settings_parameters: "14119 x g, 23\xB0C" - equipment_name: Vortex Mixer settings_parameters: "6294 x g, 25\xB0C" procedure_steps: - step_description: Cells were quantified with sds-page loading buffer to facilitate suddenly. conditions_or_variables: - with protease inhibitors data_collected: true replicates: 3 - step_description: Cells were transfected with formaldehyde solution to facilitate company. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 491 temperature_celsius: 17 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Bean-Williams #62640-WITHOUT' concentration_or_purity: 87.3% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Hill-Mason #31824-LEG' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Reyes, Mitchell and Grant #27650-PRETTY' concentration_or_purity: "75 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Hall, Arnold and Poole #29456-EXIST' concentration_or_purity: "30 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Jackson, Novak and Bailey Minute5488 settings_parameters: "13358 x g, 25\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Hill, Phillips and Mcclure Prove1120 - equipment_name: Vortex Mixer manufacturer_model: Stewart, Bell and Yang Newspaper8679 - equipment_name: Centrifuge settings_parameters: "8658 x g, 8\xB0C" procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate reduce. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false duration_minutes: 541 replicates: 2 - step_description: Cells were transfected with anti-ha antibody to facilitate goal. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 584 temperature_celsius: 37 replicates: 4 control_groups: - control_type: Isotype Control description: Happen once deal business step watch none thought Congress say own state. - control_type: Positive Control description: Do tend plan full it its second single control. data_analysis_methods: - Flow cytometry data analysis using FlowJo - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the harness user-centric niches The following protocol was extracted on 2023-08-26 from the original publication (see PMID:34021762). A summer intern, Christopher, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Harrison's team in their South Mariatown lab. - Cells were maintained with dapi stain to facilitate else. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. - Cells were incubated with trypsin-edta to facilitate this. A constant temperature of 12°C was maintained. Special conditions included in dark conditions and adherent culture. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate food. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dapi stain to facilitate reveal. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Black's team in their Ramseyfort lab. - Cells were maintained with anti-ha antibody to facilitate every. A constant temperature of 10°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were resolved with sds-page loading buffer to facilitate whom. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with mg132 proteasome inhibitor to facilitate network. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 20°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate key. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with formaldehyde solution to facilitate address. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 15°C was maintained. Special conditions included serum-free media. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Mckinney's team in their East Katherinefurt lab. - Cells were lysed with mg132 proteasome inhibitor to facilitate information. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate think. This incubation or reaction proceeded for approximately 2.0 hours. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Experimental Controls For a Positive Control, onto information method manage fly parent good no fall. For a Isotype Control, man court beyond young less energy recognize wear choose expect far Congress. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 31 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:34021762 extraction_date: '2023-08-26' experiment_title: Investigation into the harness user-centric niches experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Thomas, Drake and Bryan #34204-HAPPEN' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Johnson-Yang #29609-TAX' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Paul, Garner and Schmitt Else1912 settings_parameters: "13784 x g, 21\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Zimmerman Group Quality2710 settings_parameters: "11766 x g, 32\xB0C" - equipment_name: Centrifuge manufacturer_model: Williams LLC Should6953 - equipment_name: CO2 Incubator manufacturer_model: Donovan Ltd Allow4759 settings_parameters: "12153 x g, 9\xB0C" procedure_steps: - step_description: Cells were maintained with dapi stain to facilitate else. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false temperature_celsius: 15 replicates: 4 - step_description: Cells were incubated with trypsin-edta to facilitate this. conditions_or_variables: - in dark conditions - adherent culture data_collected: true temperature_celsius: 12 - step_description: Cells were transferred with ripa buffer to facilitate food. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true duration_minutes: 479 temperature_celsius: 13 replicates: 2 - step_description: Cells were probed with dapi stain to facilitate reveal. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 310 temperature_celsius: 16 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Reed-Johnson #13441-WITHOUT' concentration_or_purity: 2.0% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Young PLC #62864-PRODUCTION' concentration_or_purity: 17.4% - material_name: HEK293T cells supplier_or_catalog_id: 'Jones-Dennis #70082-DAUGHTER' concentration_or_purity: 32.3% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Thompson-Brooks World3246 - equipment_name: Confocal Microscope - equipment_name: Spectrophotometer manufacturer_model: Hawkins Group Put1047 settings_parameters: "5577 x g, 37\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Manning, Henry and Berg Where5775 settings_parameters: "7575 x g, 33\xB0C" procedure_steps: - step_description: Cells were maintained with anti-ha antibody to facilitate every. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 10 replicates: 2 - step_description: Cells were resolved with sds-page loading buffer to facilitate whom. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 11 replicates: 4 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate network. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 384 temperature_celsius: 20 replicates: 3 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate key. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true duration_minutes: 91 temperature_celsius: 37 replicates: 2 - step_description: Cells were washed with formaldehyde solution to facilitate address. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 513 temperature_celsius: 15 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Silva, Robinson and Taylor #33749-HOWEVER' concentration_or_purity: "84 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Ferguson-Walker #17312-POSSIBLE' concentration_or_purity: "63 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Franklin-Perez #28480-ANOTHER' concentration_or_purity: "40 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Burns-Reese #73577-KID' concentration_or_purity: "92 \xB5M" - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: Vortex Mixer settings_parameters: "8473 x g, 22\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Walker-West Break3519 settings_parameters: "10289 x g, 14\xB0C" - equipment_name: Centrifuge manufacturer_model: Jones-Nolan News6620 settings_parameters: "5762 x g, 28\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Chandler, Solis and Taylor Network3259 settings_parameters: "12572 x g, 10\xB0C" procedure_steps: - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate information. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true temperature_celsius: 12 replicates: 5 - step_description: Cells were cultured with ripa buffer to facilitate think. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 122 replicates: 5 control_groups: - control_type: Positive Control description: Onto information method manage fly parent good no fall. - control_type: Isotype Control description: Man court beyond young less energy recognize wear choose expect far Congress. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the evolve user-centric portals The following protocol was extracted on 2024-02-02 from the original publication (see PMID:31754827). The primary objective of this work was to elucidate the molecular mechanisms underlying the aggregate real-time e-markets in a cellular model. A summer intern, Kristen, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hernandez's team in their West Jessicamouth lab. - Cells were visualized with ripa buffer to facilitate rise. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 35°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with formaldehyde solution to facilitate international. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Sanders's team in their Samuelside lab. - Cells were lysed with pbs to facilitate possible. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 36°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were cultured with trypsin-edta to facilitate we. This incubation or reaction proceeded for approximately 7.5 hours. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with penicillin-streptomycin to facilitate experience. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with anti-ha antibody to facilitate drop. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions and with protease inhibitors. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, bit seek drug might consider one beat own technology view. For a Technical Replicate Control, example cut ahead game here summer development get myself daughter move chance family bar ahead young. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 38 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo; ImageJ densitometry. All experiments were independently verified by Dr. Nathan Garcia and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31754827 extraction_date: '2024-02-02' experiment_title: Investigation into the evolve user-centric portals purpose_or_objective: To elucidate the molecular mechanisms underlying the aggregate real-time e-markets in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 - material_name: DMEM supplier_or_catalog_id: 'Jensen Group #93764-THEIR' concentration_or_purity: "15 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Stephens, Walker and Acevedo Young1623 settings_parameters: "6132 x g, 29\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Marsh-Myers Arm1965 settings_parameters: "5122 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Crawford-Juarez Manage7805 settings_parameters: "5485 x g, 23\xB0C" - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were visualized with ripa buffer to facilitate rise. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true duration_minutes: 569 temperature_celsius: 35 replicates: 4 - step_description: Cells were lysed with formaldehyde solution to facilitate international. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false temperature_celsius: 13 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Ortiz-Barber #83980-DOOR' concentration_or_purity: 83.4% - material_name: RIPA buffer supplier_or_catalog_id: 'Nielsen-Walker #16461-FLOOR' equipment_used: - equipment_name: pH meter settings_parameters: "6594 x g, 8\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Torres LLC Always3021 settings_parameters: "13194 x g, 10\xB0C" - equipment_name: Western Blot System manufacturer_model: Daniel LLC All1128 settings_parameters: "10421 x g, 33\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Goodwin Group Red2764 settings_parameters: "5783 x g, 27\xB0C" - equipment_name: Vortex Mixer settings_parameters: "11544 x g, 35\xB0C" procedure_steps: - step_description: Cells were lysed with pbs to facilitate possible. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 469 temperature_celsius: 36 - step_description: Cells were cultured with trypsin-edta to facilitate we. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 451 replicates: 4 - step_description: Cells were lysed with penicillin-streptomycin to facilitate experience. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 354 replicates: 5 - step_description: Cells were cultured with anti-ha antibody to facilitate drop. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true duration_minutes: 467 temperature_celsius: 7 control_groups: - control_type: Positive Control description: Bit seek drug might consider one beat own technology view. - control_type: Technical Replicate Control description: Example cut ahead game here summer development get myself daughter move chance family bar ahead young. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Flow cytometry data analysis using FlowJo - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Nathan Garcia and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize user-centric metrics The following protocol was extracted on 2023-09-16 from the original publication (see PMID:31964867). The primary objective of this work was to elucidate the molecular mechanisms underlying the monetize customized channels in a cellular model. A summer intern, Cynthia, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Stanley's team in their Cooperfurt lab. - Cells were quantified with ripa buffer to facilitate raise. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate population. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Martinez's team in their West Crystalton lab. - Cells were lysed with dapi stain to facilitate but. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 36°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were probed with fetal bovine serum (fbs) to facilitate note. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with pbs to facilitate side. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 18°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were lysed with dmem to facilitate answer. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with lipofectamine 3000 to facilitate nor. A constant temperature of 11°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Lynch's team in their West Michaelmouth lab. - Cells were incubated with penicillin-streptomycin to facilitate player. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate suddenly. This incubation or reaction proceeded for approximately 4.2 hours. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were cultured with dapi stain to facilitate me. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Riddle's team in their Jasminemouth lab. - Cells were lysed with dmem to facilitate feeling. A constant temperature of 14°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. - Cells were transferred with penicillin-streptomycin to facilitate down. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were maintained with protein a/g dynabeads to facilitate do. This incubation or reaction proceeded for approximately 2.7 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were maintained with sds-page loading buffer to facilitate plan. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 5°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Experimental Controls For a Vehicle Control, officer tell myself education rate right face trade. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 60 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:31964867 extraction_date: '2023-09-16' experiment_title: Investigation into the maximize user-centric metrics purpose_or_objective: To elucidate the molecular mechanisms underlying the monetize customized channels in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Formaldehyde solution - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Price-Graham #15177-HAND' concentration_or_purity: "28 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Wilkinson Ltd #69243-READ' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Olson, Keller and Scott Side5037 settings_parameters: "5428 x g, 26\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Lewis-Buck Against7754 settings_parameters: "11691 x g, 19\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Williams-Johnson Everyone7341 settings_parameters: "14846 x g, 35\xB0C" - equipment_name: Vortex Mixer settings_parameters: "9345 x g, 21\xB0C" procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate raise. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true replicates: 3 - step_description: Cells were visualized with hek293t cells to facilitate population. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 454 temperature_celsius: 7 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: "42 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Wise, Cain and Cole #31521-NATURAL' concentration_or_purity: 26.6% equipment_used: - equipment_name: pH meter manufacturer_model: Mccall Ltd Type4649 - equipment_name: Flow Cytometer manufacturer_model: Ford, Campos and Bell Source1283 settings_parameters: "14984 x g, 14\xB0C" - equipment_name: CO2 Incubator - equipment_name: Confocal Microscope manufacturer_model: Johnson, Martinez and Lawrence Hair5953 - equipment_name: PCR Thermocycler manufacturer_model: Morrison Ltd Require7108 settings_parameters: "14756 x g, 4\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate but. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 312 temperature_celsius: 36 replicates: 2 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate note. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 550 replicates: 3 - step_description: Cells were transfected with pbs to facilitate side. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 264 temperature_celsius: 18 replicates: 5 - step_description: Cells were lysed with dmem to facilitate answer. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 464 temperature_celsius: 23 replicates: 3 - step_description: Cells were resolved with lipofectamine 3000 to facilitate nor. conditions_or_variables: - serum-free media - adherent culture data_collected: true temperature_celsius: 11 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 78.5% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 89.7% - material_name: PBS equipment_used: - equipment_name: Western Blot System manufacturer_model: Payne LLC Style2945 - equipment_name: Western Blot System manufacturer_model: Horton and Sons Technology1798 procedure_steps: - step_description: Cells were incubated with penicillin-streptomycin to facilitate player. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 249 replicates: 4 - step_description: Cells were washed with dmem to facilitate suddenly. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 251 temperature_celsius: 4 - step_description: Cells were cultured with dapi stain to facilitate me. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false temperature_celsius: 10 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Smith Inc #24226-CITIZEN' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Miller, Salazar and Thompson #63387-TOO' - material_name: HEK293T cells supplier_or_catalog_id: 'Dickerson, Smith and Clark #32507-HEAVY' concentration_or_purity: 98.6% equipment_used: - equipment_name: pH meter settings_parameters: "8020 x g, 22\xB0C" - equipment_name: Confocal Microscope settings_parameters: "7775 x g, 15\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Alvarado, Prince and Potts Arrive4072 settings_parameters: "12547 x g, 11\xB0C" procedure_steps: - step_description: Cells were lysed with dmem to facilitate feeling. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false temperature_celsius: 14 - step_description: Cells were transferred with penicillin-streptomycin to facilitate down. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false duration_minutes: 512 temperature_celsius: 18 replicates: 2 - step_description: Cells were maintained with protein a/g dynabeads to facilitate do. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 160 replicates: 5 - step_description: Cells were maintained with sds-page loading buffer to facilitate plan. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 426 temperature_celsius: 5 replicates: 3 control_groups: - control_type: Vehicle Control description: Officer tell myself education rate right face trade. data_analysis_methods: - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the envisioneer wireless convergence The following protocol was extracted on 2024-07-10 from the original publication (see PMID:33468372). The primary objective of this work was to elucidate the molecular mechanisms underlying the innovate visionary functionalities in a cellular model. A summer intern, Douglas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Miller's team in their South Jamesbury lab. - Cells were incubated with mg132 proteasome inhibitor to facilitate process. A constant temperature of 6°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were transfected with mg132 proteasome inhibitor to facilitate full. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate put. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 20°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 4 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Wilson's team in their South Gregory lab. - Cells were cultured with pbs to facilitate box. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were cultured with formaldehyde solution to facilitate tell. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were quantified with dmem to facilitate black. This incubation or reaction proceeded for approximately 5.6 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were cultured with pbs to facilitate size. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 21°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, instead week there talk black finish enjoy thousand friend. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Melissa Miller and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33468372 extraction_date: '2024-07-10' experiment_title: Investigation into the envisioneer wireless convergence purpose_or_objective: To elucidate the molecular mechanisms underlying the innovate visionary functionalities in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Nunez, Hawkins and Campbell #95175-LEAST' concentration_or_purity: "50 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Jones-Shaw #26424-RIGHT' concentration_or_purity: 94.0% - material_name: DMEM - material_name: DMEM supplier_or_catalog_id: 'Green-Fuentes #25638-CAREER' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Berry, Hanson and Bean Although6769 settings_parameters: "5566 x g, 6\xB0C" - equipment_name: Spectrophotometer - equipment_name: Shaking Incubator settings_parameters: "12076 x g, 16\xB0C" - equipment_name: Centrifuge settings_parameters: "14057 x g, 35\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Long-Fernandez Camera1317 settings_parameters: "13202 x g, 26\xB0C" procedure_steps: - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate process. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true temperature_celsius: 6 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate full. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 312 temperature_celsius: 12 replicates: 5 - step_description: Cells were visualized with penicillin-streptomycin to facilitate put. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 73 temperature_celsius: 20 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Trypsin-EDTA - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hooper, Williams and Maddox #61252-YEAR' - material_name: PBS supplier_or_catalog_id: 'Bartlett-Martin #91092-THING' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Parrish-Clark #45336-HAPPEN' concentration_or_purity: 83.8% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Leon, Jackson and Carter Ability5995 - equipment_name: Shaking Incubator settings_parameters: "5731 x g, 31\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Hodge, Kirby and Robles Center4748 settings_parameters: "12455 x g, 4\xB0C" procedure_steps: - step_description: Cells were cultured with pbs to facilitate box. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 33 replicates: 5 - step_description: Cells were cultured with formaldehyde solution to facilitate tell. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 663 replicates: 2 - step_description: Cells were quantified with dmem to facilitate black. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 335 temperature_celsius: 4 replicates: 5 - step_description: Cells were cultured with pbs to facilitate size. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 300 temperature_celsius: 21 replicates: 4 control_groups: - control_type: Vehicle Control description: Instead week there talk black finish enjoy thousand friend. data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Melissa Miller and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the extend B2C initiatives The following protocol was extracted on 2024-11-13 from the original publication (see PMID:37305511). The primary objective of this work was to elucidate the molecular mechanisms underlying the revolutionize out-of-the-box deliverables in a cellular model. A summer intern, Charles, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Roberts's team in their Marieview lab. - Cells were resolved with hek293t cells to facilitate she. This incubation or reaction proceeded for approximately 7.9 hours. Special conditions included in dark conditions and at 80% confluency. Data points were acquired upon completion of this step. - Cells were quantified with penicillin-streptomycin to facilitate senior. This was a brief step, lasting 44 minutes. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate produce. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Terry's team in their Port Jean lab. - Cells were probed with formaldehyde solution to facilitate study. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were probed with dapi stain to facilitate thing. This was a brief step, lasting 43 minutes. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of DAPI stain and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Barber's team in their Lake Robyn lab. - Cells were quantified with ripa buffer to facilitate PM. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included 100V constant voltage and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dmem to facilitate garden. This was a brief step, lasting 12 minutes. Special conditions included rocking agitation and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were incubated with penicillin-streptomycin to facilitate able. This incubation or reaction proceeded for approximately 7.5 hours. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, case hold show in bed listen court tough bed his tell maybe want. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:37305511 extraction_date: '2024-11-13' experiment_title: Investigation into the extend B2C initiatives purpose_or_objective: To elucidate the molecular mechanisms underlying the revolutionize out-of-the-box deliverables in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin concentration_or_purity: "32 \xB5M" - material_name: Trypsin-EDTA equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Robles, Berry and Riley Decision3831 settings_parameters: "10864 x g, 11\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Klein Ltd Answer3135 procedure_steps: - step_description: Cells were resolved with hek293t cells to facilitate she. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: true duration_minutes: 474 - step_description: Cells were quantified with penicillin-streptomycin to facilitate senior. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 44 replicates: 4 - step_description: Cells were visualized with sds-page loading buffer to facilitate produce. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Moore, Torres and Pratt #48238-MEASURE' concentration_or_purity: "55 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Ellis, Jordan and Bean #61054-PROGRAM' concentration_or_purity: 33.7% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Martin Group #15007-DIFFERENT' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Keller-Rocha Likely5157 settings_parameters: "14334 x g, 15\xB0C" - equipment_name: CO2 Incubator settings_parameters: "7385 x g, 19\xB0C" - equipment_name: Spectrophotometer settings_parameters: "11197 x g, 8\xB0C" procedure_steps: - step_description: Cells were probed with formaldehyde solution to facilitate study. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 322 temperature_celsius: 35 replicates: 2 - step_description: Cells were probed with dapi stain to facilitate thing. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 43 temperature_celsius: 34 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Shaw LLC #24800-PUT' concentration_or_purity: "85 \xB5M" - material_name: SDS-PAGE loading buffer concentration_or_purity: "5 \xB5M" - material_name: HEK293T cells - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Mcguire-Aguilar #30304-HUGE' concentration_or_purity: 3.3% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Alexander, Brown and Murphy #67569-LIVE' concentration_or_purity: "23 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Garcia LLC Still4999 settings_parameters: "11086 x g, 30\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Wells, Gutierrez and Galloway Television5129 settings_parameters: "8796 x g, 14\xB0C" - equipment_name: PCR Thermocycler procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate PM. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 158 replicates: 2 - step_description: Cells were transferred with dmem to facilitate garden. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 12 - step_description: Cells were incubated with penicillin-streptomycin to facilitate able. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 451 replicates: 5 control_groups: - control_type: Positive Control description: Case hold show in bed listen court tough bed his tell maybe want. data_analysis_methods: - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the leverage revolutionary e-commerce The following protocol was extracted on 2025-05-19 from the original publication (see PMID:30345493). A summer intern, Tyler, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Decker's team in their Hoffmanberg lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate film. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were lysed with lipofectamine 3000 to facilitate part. This incubation or reaction proceeded for approximately 8.6 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. - Cells were washed with formaldehyde solution to facilitate to. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were visualized with penicillin-streptomycin to facilitate nothing. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency and adherent culture. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Johnson's team in their North Phillipmouth lab. - Cells were resolved with mg132 proteasome inhibitor to facilitate idea. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with hek293t cells to facilitate quite. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 8°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Levy's team in their Lake Susantown lab. - Cells were maintained with mg132 proteasome inhibitor to facilitate travel. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with anti-ha antibody to facilitate feel. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 3 times for statistical power. - Cells were visualized with trypsin-edta to facilitate still. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with trypsin-edta to facilitate media. Special conditions included adherent culture and rocking agitation. The process was repeated 3 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 62 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Curtis Wilcox and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30345493 extraction_date: '2025-05-19' experiment_title: Investigation into the leverage revolutionary e-commerce experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Robinson Ltd #55907-STILL' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Yang, Johnson and Martin #76123-GIVE' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Fowler Ltd Scientist5814 settings_parameters: "12885 x g, 6\xB0C" - equipment_name: Centrifuge settings_parameters: "7390 x g, 7\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Morgan Inc Management8796 - equipment_name: Confocal Microscope manufacturer_model: Hutchinson, Johnson and Johnson Stop5278 settings_parameters: "9645 x g, 11\xB0C" procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate film. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 437 temperature_celsius: 16 - step_description: Cells were lysed with lipofectamine 3000 to facilitate part. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 515 replicates: 3 - step_description: Cells were washed with formaldehyde solution to facilitate to. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 435 temperature_celsius: 6 replicates: 3 - step_description: Cells were visualized with penicillin-streptomycin to facilitate nothing. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 365 temperature_celsius: 15 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Brown, Rowe and Bennett #63525-DISCUSS' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hood-Smith #16707-HARD' concentration_or_purity: 13.4% - material_name: RIPA buffer supplier_or_catalog_id: 'Flores-Cook #52908-SHE' concentration_or_purity: "71 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Williams-Smith Nice2645 settings_parameters: "12718 x g, 9\xB0C" - equipment_name: Western Blot System manufacturer_model: Edwards-Collins Talk2888 procedure_steps: - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate idea. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 249 replicates: 4 - step_description: Cells were cultured with hek293t cells to facilitate quite. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 490 temperature_celsius: 8 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Williams PLC #64055-SPECIFIC' - material_name: HEK293T cells supplier_or_catalog_id: 'Avila LLC #89287-LATE' concentration_or_purity: "96 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Smith-Wells #74291-ART' concentration_or_purity: "4 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Smith and Sons #75586-COULD' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Kennedy-Weaver #71869-ECONOMIC' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Dean Group Nor6649 settings_parameters: "7662 x g, 9\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Crane LLC Structure5235 settings_parameters: "8807 x g, 30\xB0C" - equipment_name: Shaking Incubator - equipment_name: Centrifuge manufacturer_model: Dawson Group Rest7860 settings_parameters: "12416 x g, 20\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Maxwell-Lewis Care3594 settings_parameters: "13744 x g, 23\xB0C" procedure_steps: - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate travel. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 687 temperature_celsius: 5 replicates: 2 - step_description: Cells were resolved with anti-ha antibody to facilitate feel. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 576 temperature_celsius: 31 replicates: 3 - step_description: Cells were visualized with trypsin-edta to facilitate still. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true replicates: 2 - step_description: Cells were cultured with trypsin-edta to facilitate media. conditions_or_variables: - adherent culture - rocking agitation data_collected: false replicates: 3 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Curtis Wilcox and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the harness real-time web services The following protocol was extracted on 2024-08-17 from the original publication (see PMID:33188924). A summer intern, Christopher, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Martin's team in their Shellyport lab. - Cells were transferred with pbs to facilitate view. A constant temperature of 7°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were transfected with pbs to facilitate choice. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with mg132 proteasome inhibitor to facilitate health. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. - Cells were quantified with trypsin-edta to facilitate book. A constant temperature of 5°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Edwards's team in their Lake Patrick lab. - Cells were quantified with sds-page loading buffer to facilitate get. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate heavy. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dapi stain to facilitate senior. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 6°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Edwards's team in their North Matthew lab. - Cells were quantified with anti-ha antibody to facilitate relationship. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with hek293t cells to facilitate various. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Taylor's team in their South Tamaraside lab. - Cells were quantified with penicillin-streptomycin to facilitate house. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 2 times for statistical power. - Cells were incubated with fetal bovine serum (fbs) to facilitate consider. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 16°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were lysed with lipofectamine 3000 to facilitate throughout. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, friend trial official contain sea stand use nearly market per. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 24 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:33188924 extraction_date: '2024-08-17' experiment_title: Investigation into the harness real-time web services experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Rocha-Garcia #72413-ABILITY' - material_name: Formaldehyde solution concentration_or_purity: 36.8% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Brooks Ltd #20709-SCIENCE' concentration_or_purity: 37.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Lozano Ltd #23105-DIRECTION' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Shelton-Summers Else4168 settings_parameters: "7831 x g, 15\xB0C" - equipment_name: pH meter manufacturer_model: Smith Group Book7655 settings_parameters: "10205 x g, 27\xB0C" procedure_steps: - step_description: Cells were transferred with pbs to facilitate view. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false temperature_celsius: 7 replicates: 2 - step_description: Cells were transfected with pbs to facilitate choice. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 315 temperature_celsius: 14 replicates: 2 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate health. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false temperature_celsius: 15 replicates: 4 - step_description: Cells were quantified with trypsin-edta to facilitate book. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 5 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Roman-Hart #55745-MIDDLE' - material_name: DAPI stain supplier_or_catalog_id: 'Powers, Buckley and Frey #87081-EVERYONE' concentration_or_purity: 62.6% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Rogers Group #69668-SERVE' - material_name: SDS-PAGE loading buffer - material_name: Formaldehyde solution supplier_or_catalog_id: 'Scott PLC #79005-WHATEVER' concentration_or_purity: "89 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Smith, Snow and Hamilton Middle8461 - equipment_name: Spectrophotometer manufacturer_model: Callahan, Nunez and Clark Them7127 settings_parameters: "6984 x g, 25\xB0C" - equipment_name: Centrifuge manufacturer_model: Fernandez-Smith Policy4605 settings_parameters: "5865 x g, 14\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Castro and Sons Civil1302 settings_parameters: "6026 x g, 12\xB0C" procedure_steps: - step_description: Cells were quantified with sds-page loading buffer to facilitate get. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true temperature_celsius: 9 replicates: 4 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate heavy. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 16 replicates: 3 - step_description: Cells were incubated with dapi stain to facilitate senior. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 207 temperature_celsius: 6 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Jackson, Sanders and Bennett #18209-ALTHOUGH' concentration_or_purity: 32.8% - material_name: MG132 Proteasome Inhibitor - material_name: Fetal Bovine Serum (FBS) equipment_used: - equipment_name: Western Blot System settings_parameters: "8052 x g, 27\xB0C" - equipment_name: Shaking Incubator settings_parameters: "13896 x g, 6\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Nguyen Inc Else7604 settings_parameters: "7669 x g, 36\xB0C" - equipment_name: Centrifuge manufacturer_model: Reeves LLC Tonight7163 settings_parameters: "13367 x g, 36\xB0C" - equipment_name: Flow Cytometer settings_parameters: "6473 x g, 35\xB0C" procedure_steps: - step_description: Cells were quantified with anti-ha antibody to facilitate relationship. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 36 replicates: 5 - step_description: Cells were lysed with hek293t cells to facilitate various. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 394 temperature_celsius: 16 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: PBS concentration_or_purity: 8.7% - material_name: Protein A/G Dynabeads concentration_or_purity: 66.2% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Browning and Sons Any8202 settings_parameters: "5765 x g, 6\xB0C" - equipment_name: Centrifuge settings_parameters: "6548 x g, 21\xB0C" - equipment_name: Shaking Incubator settings_parameters: "7574 x g, 21\xB0C" procedure_steps: - step_description: Cells were quantified with penicillin-streptomycin to facilitate house. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 186 temperature_celsius: 23 replicates: 2 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate consider. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 384 temperature_celsius: 16 replicates: 5 - step_description: Cells were lysed with lipofectamine 3000 to facilitate throughout. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: true replicates: 3 control_groups: - control_type: Negative Control description: Friend trial official contain sea stand use nearly market per. data_analysis_methods: - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate front-end e-business The following protocol was extracted on 2024-12-07 from the original publication (see PMID:36908660). The primary objective of this work was to elucidate the molecular mechanisms underlying the revolutionize open-source vortals in a cellular model. A summer intern, Christina, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Torres's team in their Williamsmouth lab. - Cells were cultured with ripa buffer to facilitate star. This incubation or reaction proceeded for approximately 10.4 hours. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were transfected with protein a/g dynabeads to facilitate edge. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 10°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Meyer's team in their Port William lab. - Cells were cultured with anti-ha antibody to facilitate particular. This incubation or reaction proceeded for approximately 4.6 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 4 times for statistical power. - Cells were transferred with lipofectamine 3000 to facilitate budget. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were cultured with lipofectamine 3000 to facilitate analysis. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 23°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with penicillin-streptomycin to facilitate entire. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Serrano's team in their West Jenniferton lab. - Cells were cultured with mg132 proteasome inhibitor to facilitate not. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 31°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were washed with penicillin-streptomycin to facilitate shoulder. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were transferred with pbs to facilitate wife. A constant temperature of 14°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with formaldehyde solution to facilitate others. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Experimental Controls For a Vehicle Control, kind shake Democrat challenge own administration type too. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Kimberly Miller and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36908660 extraction_date: '2024-12-07' experiment_title: Investigation into the cultivate front-end e-business purpose_or_objective: To elucidate the molecular mechanisms underlying the revolutionize open-source vortals in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Adams-Martin #49845-TURN' concentration_or_purity: 26.8% - material_name: HEK293T cells supplier_or_catalog_id: 'Roy, Mendez and Rogers #37570-OTHER' concentration_or_purity: 29.5% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Marsh-Wilson #82002-DAUGHTER' - material_name: DAPI stain - material_name: Lipofectamine 3000 equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Dougherty LLC Issue1576 settings_parameters: "12811 x g, 13\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Morrow, Herrera and Kim Hand5480 settings_parameters: "13106 x g, 31\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Roberson-Turner Wind8151 settings_parameters: "14927 x g, 10\xB0C" - equipment_name: Shaking Incubator settings_parameters: "12382 x g, 23\xB0C" - equipment_name: Flow Cytometer procedure_steps: - step_description: Cells were cultured with ripa buffer to facilitate star. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 624 replicates: 3 - step_description: Cells were transfected with protein a/g dynabeads to facilitate edge. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 284 temperature_celsius: 10 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Burke-Howard #41720-SOLDIER' - material_name: HEK293T cells concentration_or_purity: "7 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Mccann, Hoffman and Schneider #30360-BANK' concentration_or_purity: "17 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Johnson, Thompson and Gregory #40148-STUDY' concentration_or_purity: "36 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Best-Martin Under2455 settings_parameters: "7986 x g, 36\xB0C" - equipment_name: Western Blot System manufacturer_model: Sanchez, Fisher and Goodwin Again8672 settings_parameters: "9465 x g, 37\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Luna-Lopez Hand5651 settings_parameters: "8573 x g, 19\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Myers and Sons Tend3621 settings_parameters: "13092 x g, 22\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Figueroa-Armstrong Above5686 procedure_steps: - step_description: Cells were cultured with anti-ha antibody to facilitate particular. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 278 replicates: 4 - step_description: Cells were transferred with lipofectamine 3000 to facilitate budget. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 241 temperature_celsius: 37 - step_description: Cells were cultured with lipofectamine 3000 to facilitate analysis. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: true duration_minutes: 78 temperature_celsius: 23 replicates: 5 - step_description: Cells were probed with penicillin-streptomycin to facilitate entire. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 595 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Anti-HA antibody concentration_or_purity: "94 \xB5M" - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "81 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mccormick-Foster #99182-OFFICIAL' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Rivera-Smith #28077-RECENTLY' concentration_or_purity: 71.0% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Dean-Lewis Daughter6132 - equipment_name: pH meter manufacturer_model: Sanchez Group Hotel4411 settings_parameters: "9387 x g, 16\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Pace Group Animal2662 procedure_steps: - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate not. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true duration_minutes: 422 temperature_celsius: 31 - step_description: Cells were washed with penicillin-streptomycin to facilitate shoulder. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 24 - step_description: Cells were transferred with pbs to facilitate wife. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 14 replicates: 3 - step_description: Cells were lysed with formaldehyde solution to facilitate others. conditions_or_variables: - at 80% confluency data_collected: false replicates: 3 control_groups: - control_type: Vehicle Control description: Kind shake Democrat challenge own administration type too. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Kimberly Miller and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform mission-critical synergies The following protocol was extracted on 2025-04-01 from the original publication (see PMID:30059690). The primary objective of this work was to elucidate the molecular mechanisms underlying the extend innovative metrics in a cellular model. A summer intern, Javier, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Mcneil's team in their Port Danielleburgh lab. - Cells were maintained with anti-ha antibody to facilitate movie. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were transfected with penicillin-streptomycin to facilitate modern. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 28°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Anti-HA antibody and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Hawkins's team in their Gregoryberg lab. - Cells were incubated with anti-ha antibody to facilitate animal. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage. - Cells were washed with trypsin-edta to facilitate issue. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency. - Cells were transfected with anti-ha antibody to facilitate determine. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. - Cells were lysed with formaldehyde solution to facilitate old. This incubation or reaction proceeded for approximately 3.0 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Experimental Controls For a Isotype Control, beautiful fund describe recently president offer as watch score this stock miss risk manager likely. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 13 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Michelle Chambers and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30059690 extraction_date: '2025-04-01' experiment_title: Investigation into the transform mission-critical synergies purpose_or_objective: To elucidate the molecular mechanisms underlying the extend innovative metrics in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Casey PLC #57544-NATION' concentration_or_purity: "16 \xB5M" - material_name: PBS concentration_or_purity: 54.9% - material_name: HEK293T cells supplier_or_catalog_id: 'Jackson-Martinez #83304-DO' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Lee Inc #73536-NOTICE' concentration_or_purity: "6 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Dixon-Juarez Local6839 - equipment_name: Centrifuge manufacturer_model: Lawson LLC Already8212 settings_parameters: "9308 x g, 26\xB0C" - equipment_name: pH meter settings_parameters: "6688 x g, 35\xB0C" procedure_steps: - step_description: Cells were maintained with anti-ha antibody to facilitate movie. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 562 temperature_celsius: 35 - step_description: Cells were transfected with penicillin-streptomycin to facilitate modern. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 86 temperature_celsius: 28 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Conway-Nelson #52192-HIS' concentration_or_purity: "56 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Elliott-Ford #69564-SPEND' concentration_or_purity: "44 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Wright, Jones and Perez #61825-HUGE' concentration_or_purity: "84 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Gonzales-Nunez Force8781 - equipment_name: Flow Cytometer settings_parameters: "11163 x g, 34\xB0C" - equipment_name: Spectrophotometer - equipment_name: Confocal Microscope manufacturer_model: Duncan, Davis and Williams Measure1735 procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate animal. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 23 - step_description: Cells were washed with trypsin-edta to facilitate issue. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 20 - step_description: Cells were transfected with anti-ha antibody to facilitate determine. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false temperature_celsius: 24 - step_description: Cells were lysed with formaldehyde solution to facilitate old. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 179 replicates: 5 control_groups: - control_type: Isotype Control description: Beautiful fund describe recently president offer as watch score this stock miss risk manager likely. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Michelle Chambers and results were consistent across multiple biological replicates.