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The patients underwent patch tests with allergens from the Chemotechnique Diagnostics Malmö, Sweden, including nickel, chromium, cobalt, palladium, copper, aluminum. | W1583351496 | [
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In addition, allergens, such as titanium, vanadium and molybdenum prepared by chemical laboratory in the Nofer Institute of Occupational Medicine, Lódiź, Poland, were introduced. | W1583351496 | [
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Owing to the exchange of prosthesis the complaints subsided, including healing up skin fibulas. | W1583351496 | [
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The result of the study showed that 6.2 of the students prefer visual mode, 11.3 prefer aural mode, 2.3 prefer reading writing mode and 8.1 prefer kinesthetic mode, and the rest 72.1 prefer multiple learning styles. | W2187029704 | [
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The result of this study can provide useful information for improving the quality of the teaching and learning process in Semarang State Polytechnic. | W2187029704 | [
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Introduction Neutrophils play a major role in sepsisinduced organ dysfunction, especially in the lung. | W1891465115 | [
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HMGB1 has emerged as a late cytokine and is implicated in the perpetuation of inflammatory stimulus and organ dysfunction development as well. | W1891465115 | [
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Objectives To evaluate the differences of gene expression and activation of NFκB, Akt, and p38MAPK in blood neutrophils from septic patients exposed to HMGB1 and LPS and to compare response patterns between blood neutrophils from patients and healthy volunteers. | W1891465115 | [
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Methods Twentytwo sepsisinduced acute lung injury patients and 34 healthy volunteers were enrolled in this study. | W1891465115 | [
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... | Synthesising sources: supporting evidence or ideas | 739,294 |
The primary clinical variables collected were the 28day survival and the presence of shock at ICU admission. | W1891465115 | [
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Peripheral blood was obtained and neutrophils were isolated by plasmapercoll gradients after dextran sedimentation of erythrocytes. | W1891465115 | [
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Neutrophils were resuspended in RPMI and cultured with or without 1000 ngml rHMGB1 or with or without 100 ngml LPS for 15, 30, and 60 min.The electrophoretic mobility shift assay technique was used to measure the NFκB translocation, while western blot analysis was used to determine Akt phosphorylation and an ELISA was ... | W1891465115 | [
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Microarray analysis was used to evaluate the neutrophil gene expression in unstimulated neutrophils and after either HMGB1 stimulus or LPS stimulus. | W1891465115 | [
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P 0.05 was considered significant. | W1891465115 | [
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"s... | Describing the process: expressing purpose with for | 743,835.0625 |
Results Although with some similarities, HMGB1 and LPS induced distinct patterns of gene expression in peripheral blood neutrophils from septic patients. | W1891465115 | [
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A Venn diagram Fig. 1 displays genes upregulated greater than twofold that are both common and unique after both stimuli. | W1891465115 | [
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Using functional ontology, the genes upregulated by both HMGB1 and LPS primarily consisted of cytokines, chemokines, coagulationrelated proteins, phosphatases, and transcriptional regulators factors. | W1891465115 | [
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Importantly, while HMGB1 induced an HMGB1related gene downregulation, LPS did not induce any changes in HMGB1 gene expression in these patients. | W1891465115 | [
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Regarding intracellular activation, both HMGB1 and LPS increased translocation of NFκB and the phosphorylation of Akt and p38MAPK in neutrophils from septic patients. | W1891465115 | [
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However, there were some differences in terms of the degree and kinetics of activation between neutrophils cultured with LPS and HMGB1 Fig. 2. | W1891465115 | [
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... | Establishing the importance of the topic for the discipline | 714,118 |
There are no important differences in terms of intracellular activation when we compared neutrophils from septic patients with those from volunteers. | W1891465115 | [
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Finally, neither NFκB translocation nor kinase phosphorylation was associated with sepsis severity. | W1891465115 | [
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However, the majority of genes in unstimulated neutrophils and after HMGB1 had a higher expression in mild patients. | W1891465115 | [
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In contrast, CCL20, CCRL2, CIAS1, PTGER, PTX3, and MAP3K8 had a higher expression in severe patients only after LPS stimulus. | W1891465115 | [
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{
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ConclusionAlthough with some similarities, HMGB1 and LPS induced distinct pattern of gene expression in neutrophils from septic patients. | W1891465115 | [
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Both stimuli were able to increase intracellular activation and this activation was similar to that found in neutrophils from volunteers, showing that even after sepsis stimulus the neutrophil keeps its ability to respond to a second hit. | W1891465115 | [
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... | Highlighting inadequacies or weaknesses of previous studies (also refer to Being Critical) | 721,060 |
Background Intratumor hypoxia, the main factor responsible for the angiogenic switch, represents one of the major events leading to tumor progression. | W2979682323 | [
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Tumor hypoxia leads to the stabilization of hypoxiainducible factor1α HIF1α which influences tumor angiogenesis. | W2979682323 | [
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"s": 745204.... | Describing the limitations of the current study | 695,676.9375 |
The new blood vessels formed by the HIF1αvascular endothelial growth factor VEGF signaling axis create the tumor microenvironment, which inhibits drug delivery to solid tumors. | W2979682323 | [
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The present study aimed to investigate the effect of Andrographis paniculata leaf extract as a powerful anticancer agent targeting the HIF1αVEGF signaling axis in mouse melanoma cell. | W2979682323 | [
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Materials and Methods We induced hypoxiamimicking conditions in mouse B16 melanoma cell with cobalt chloride. | W2979682323 | [
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"m": "Highlighting inadequacies o... | Referring to important texts in the area of interest | 728,330 |
Total RNA was isolated followed by reverse transcriptase polymerase chain reaction to study the transcriptional expressions of HIF1α and VEGF. | W2979682323 | [
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{
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"... | Indicating criteria for selection or inclusion in the study | 754,595.0625 |
Further confirmation of the transcriptional profiling was done at the protein level with Western blot analysis. | W2979682323 | [
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... | Explaining the significance of the findings or contribution of the study | 606,531 |
Expression profiling of transcriptional factors involved in the hypoxia signaling cascade was done. | W2979682323 | [
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"s": 691552.9... | Referring to a single investigation in the past: investigation prominent | 651,655 |
An immunofluorescence study was also used to confirm the results obtained from transcriptional and translational analyses. | W2979682323 | [
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Results A. paniculata leaf extract significantly downregulated the expressions of HIF1α and VEGF both at the transcriptional and translational level. | W2979682323 | [
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Sp1, p300, CBP expressions were also downregulated, whereas the expression of Sp3 was significantly upregulated by A. paniculata leaf extract in B16 melanoma cells. | W2979682323 | [
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Conclusion In the present study, A. paniculatatreated cells demonstrated lower expressions of VEGF and HIF1α both at the transcriptional and translational level. | W2979682323 | [
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The mechanism of the downregulation of HIF1α was probably through the altered expressions of transcriptional factors involved in the hypoxiasignaling cascade. | W2979682323 | [
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The aim of this study was to compare insertion torque and resonance frequency analysis of different implant systems in an animal model with loaded implants. | W144988327 | [
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Three types of Brånemark implants machined MkIII, TiUnite MkIII, and MkIV and 2 types of Straumann implants sandblasted, largegrit, acidetched SLA and titanium plasmasprayed TPS were studied. | W144988327 | [
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"s... | Commenting on the strengths of the current study | 774,168 |
Thirtytwo implants of each type n 160 were placed in 16 beagle dogs. | W144988327 | [
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Maximum insertion torque values were recorded. | W144988327 | [
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After a healing period of 8 weeks, the implants were loaded for 3 months the animals were then sacrificed. | W144988327 | [
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At placement, after healing, and at the end of the loading phase, resonance frequency analysis was performed and implant stability quotients ISQs were recorded. | W144988327 | [
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Higher insertion torque values were seen for the conical MkIV than for the MkIII. | W144988327 | [
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No difference was seen between the Brånemark and Straumann implants on the basis of ISQ value at placement. | W144988327 | [
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ISQ and insertion torque values were lower for the cylindric Straumann implants than for the selftapping implants. | W144988327 | [
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For all implant systems a significant decrease in median ISQ was observed, with a median decrease ranging from 3 to 6. | W144988327 | [
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ISQ values for selftapping implants remained stable after loading, whereas the ISQ values for nonselftapping cylinders decreased. | W144988327 | [
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The maximum insertion torque values for failed and successful implants were not significantly different. | W144988327 | [
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Significantly higher ISQ values at placement were seen for successful implants P .003. | W144988327 | [
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Based on this model for ISQ, a threshold of 65.5 was identified, with a sensitivity of 83 and specificity of 61 for prediction of implant loss. | W144988327 | [
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