IdA
stringlengths
6
21
IdB
stringlengths
6
21
labels
int64
0
2
mechanism
stringclasses
40 values
effect
stringclasses
10 values
score
float64
0.1
0.99
sentence
stringlengths
10
1.63k
signor_id
stringlengths
12
14
Q9H7Z6
P49327
1
acetylation
down-regulates quantity by destabilization
0.2
Overexpression of Myc-KAT8 increased the acetylation level of endogenous FASN by 2.2-fold (Fig. 3C). In contrast, knockdown of KAT8 decreased endogenous FASN acetylation by as much as 55%
SIGNOR-267366
P19086
P28222
2
binding
up-regulates activity
0.25
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257115
P51587
Q9BXW9
2
binding
up-regulates activity
0.812
Fanconi anemia complementation group FANCD2 protein serine 331 phosphorylation is important for fanconi anemia pathway function and BRCA2 interaction
SIGNOR-263238
Q02548
P27361
0
phosphorylation
down-regulates activity
0.247
In this study, we demonstrated that PAX5 was phosphorylated by ERK1/2 in vitro and in vivo at serines 189 and 283. This phosphorylation attenuated the transcriptional repression of BLIMP1 by PAX5.
SIGNOR-269086
Q19QW5
P52292
1
relocalization
down-regulates activity
0.2
Taken together, these data support a direct interaction between KPNA2 and ORF6 in the context of virus infection.|SARS-CoV, but not SARSdeltaORF6, retains KPNA2 at the ER/Golgi membrane.
SIGNOR-260272
Q12756
P0DP23
2
binding
up-regulates activity
0.275
To better understand how KIF1A-driven dense core vesicle (DCV) transport is regulated, we identified the KIF1A interactome and focused on three binding partners, the calcium binding protein calmodulin (CaM) and two synaptic scaffolding proteins: liprin-α and TANC2. We showed that calcium, acting via CaM, enhances KIF1A...
SIGNOR-266888
Q92731
P03372
2
binding
up-regulates
0.527
It was recently shown that er? And er? Could form a heterodimer complex both in vitro and in vivo
SIGNOR-64427
Q12933
Q8IUC6
1
polyubiquitination
up-regulates
0.433
Here, we show that the TRAF family proteins directly bind TICAM-1 and demonstrate that TRAF2 and TRAF6 bind different sites of the N-terminal TICAM-1 and accelerate its polyubiquitination. we speculate that polyubiquitination of TICAM-1 by TRAF2 and TRAF6 is required for TICAM-1 to induce IRF-3 and NF-κB activation. Th...
SIGNOR-271427
Q99683
Q9H2G2
0
phosphorylation
up-regulates
0.253
Induction of apoptosis by the ste20-like kinase slk, a germinal center kinase that activates apoptosis signal-regulating kinase and p38
SIGNOR-142665
P03372
P17612
0
phosphorylation
down-regulates
0.486
Phosphorylation of human estrogen receptor alpha by protein kinase a regulates dimerizationeralpha is phosphorylated by protein kinase a (pka) on serine-236 within the dna binding domain. Mutation of serine-236 to glutamic acid prevents dna binding by inhibiting dimerization by eralpha
SIGNOR-63984
P09471
P32238
2
binding
up-regulates activity
0.252
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257236
Q08881
P06239
0
phosphorylation
up-regulates
0.554
Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity. The major site of Lck phosphorylation on Itk was mapped to the conserved tyrosine (Tyr511) in the activation loop of the Itk kinase domain.
SIGNOR-251380
P49354
O14807
1
null
up-regulates activity
0.2
Major investments have been made to target Ras through indirect routes. Inhibition of farnesyl transferase to block Ras maturation has failed in large clinical trials.
SIGNOR-242553
Q12913
Q86YJ5
0
ubiquitination
down-regulates quantity by destabilization
0.2
MARCH9, a member of the RING-CH family of transmembrane E3 ubiquitin ligases, down-regulates CD4, major histocompatibility complex-I (MHC), and ICAM-1 in lymphoid cells. To identify novel MARCH9 substrates, we used high throughput flow cytometry and quantitative mass spectrometry by stable isotope labeling by amino aci...
SIGNOR-271535
P30518
P63092
2
binding
up-regulates activity
0.589
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-256754
Q9NPB9
O00585
2
binding
up-regulates activity
0.666
 In the present study, however, we demonstrate for the first time the concentration-dependent recruitment of β-arrestins to the atypical chemokine receptor CCX-CKR upon stimulation with CCL19, CCL21, or CCL25 using three different methodologies in various transfected cell lines.
SIGNOR-268417
O43524
O43638
0
transcriptional regulation
down-regulates quantity by repression
0.2
Fkhl18 suppressed the transcriptional activity of FoxO3a and FoxO4.
SIGNOR-261610
P21453
P31749
0
phosphorylation
up-regulates activity
0.704
Activated akt binds to edg-1 and phosphorylates the third intracellular loop at the t(236) residue. Transactivation of edg-1 by akt is not required for g(i)-dependent signaling but is indispensable for rac activation, cortical actin assembly, and chemotaxis
SIGNOR-252467
P0C6X7-PRO_0000037317
P00403
2
binding
down-regulates activity
0.2
This result suggests that the nsp10 protein could affect the activities of NADH and cytochrome oxidase II via a direct interaction while being involved in viral replication.
SIGNOR-260254
P17612
P21817
1
phosphorylation
up-regulates activity
0.326
PKA-mediated hyperphosphorylation of a conserved serine, Ser-2843 in skeletal RyR and Ser-2809 in cardiac RyR, results in an aberrant SR function during heart failure. hyperphosphorylated RyRs are leaky and therefore lead to a reduced SR Ca2+ load and impaired contractile function in heart failure
SIGNOR-250078
P32243
P14652
0
transcriptional regulation
up-regulates quantity by expression
0.259
Transactivation of the mouse OTX2 Luc constructs by the human HOXB1, HOXB2, and HOXB3 proteins. | Likewise, the construct pOTX2LucΔ−710 showed an 8-, 12-, and 6-fold increase in transcriptional activity if co-transfected with pSG-HOXB1, -HOXB2, and -HOXB3, respectively
SIGNOR-261634
P12931
O75553
1
phosphorylation
up-regulates activity
0.42
Dab1 is rapidly phosphorylated when neurons isolated from embryonic brains are stimulated with Reelin, and several tyrosines have been implicated in this response. Mice with phenylalanine substitutions of all five tyrosines (Tyr(185), Tyr(198), Tyr(200), Tyr(220), and Tyr(232)) exhibit a reeler phenotype, implying that...
SIGNOR-247080
Q9HAU5
Q92900
2
binding
up-regulates activity
0.979
UPF2 and UPF3b increase UPF1 ATPase activity
SIGNOR-265246
P19784
Q9UD71
1
phosphorylation
up-regulates activity
0.375
Study of [Plphosphate release during manual Edman degradation confirmed that the phosphorylated residues in rat DARPP-32 were Ser45 and Ser102. | Phosphorylation by casein kinase II did not affect the potency of DARPP-32 as an inhibitor of protein phosphatase-1, which depended only on phosphorylation of Thr34 by cAMP-...
SIGNOR-251018
P63092
O00254
2
binding
up-regulates activity
0.2
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-256761
P33151
Q9UQB3
2
binding
up-regulates quantity by stabilization
0.316
To clarify the role of p120 in mammalian cells, we have knocked down p120 with siRNA in cells expressing epithelial (E-), placental (P-), neuronal (N-), and vascular endothelial (VE-) cadherins. We report that each of these cadherins, as well as α- and β-catenins, were rapidly degraded in the absence of p120, resulting...
SIGNOR-252132
P42229
P24394
0
null
up-regulates
0.556
Several cytokine receptors share subchains and targets. For example, the common gamma chain (CGC) is shared by IL2, IL4, IL7, IL9 and IL15 receptors that lead to the activation of STAT5
SIGNOR-254298
O15379
Q8NHE4
1
transcriptional regulation
down-regulates quantity by repression
0.2
Consistent with previous data, HDAC3 only bound to the ATP6V0E2 promoter in the presence of ALDH2.|Taken together, our data demonstrate that in the macrophages of LDLR-KO or ALDH2 rs671 mutant, AMPK phosphorylates ALDH2 at T356, which enables its nuclear translocation. Once in the nucleus, ALDH2 binds to HDAC3 and supp...
SIGNOR-271868
Q12802
P17612
0
phosphorylation
up-regulates
0.332
Using a combination of biochemical, enzymatic, and immunofluorescence techniques, we show that the anchoring protein contributes to pkd activation in two ways: it recruits an upstream kinase pkceta and coordinates pka phosphorylation events that release activated protein kinase d. Thus, akap-lbc synchronizes pka and pk...
SIGNOR-129345
P35367
P63096
2
binding
up-regulates activity
0.2
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257050
Q05086
P51665
1
ubiquitination
down-regulates quantity by destabilization
0.271
Our experiments collectively suggest that UBE3A stimulates Wnt pathway activation by interacting with, ubiquitinating, and reducing the levels of multiple (PSMB1, PSMC2, PSMD2, and PSMD7) proteasome subunits.
SIGNOR-265134
P08514
Q99828
2
binding
up-regulates activity
0.472
The small GTPase Rac3 interacts with the integrin-binding protein CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and spreading
SIGNOR-269061
O15550
P31268
1
transcriptional regulation
up-regulates quantity by expression
0.275
Evidence for direct involvement of UTX in regulation of HOX gene activity was demonstrated through UTX knockdown experiments in HEK293T cells in which loss of UTX induced transcriptional repression of HOXA and HOXC clusters.
SIGNOR-260024
O14965
O15350
1
phosphorylation
down-regulates activity
0.449
Aurora-A inhibits p73 and p53 transactivation functions through a common molecular mechanism.|We report that Aurora-A phosphorylation of p73 at serine235 abrogates its transactivation function and causes cytoplasmic sequestration in a complex with the chaperon protein mortalin.
SIGNOR-278263
Q99665
P29459
2
binding
up-regulates
0.578
Il-12r beta 2 plays an essential role in mediating the biological functions of il-12 in mice.
SIGNOR-84361
P08887
P40189
2
binding
up-regulates
0.751
Part of the receptor for interleukin 6. Binds to il6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with il6st. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
SIGNOR-105504
Q15759
P30281
1
phosphorylation
down-regulates quantity by destabilization
0.2
P38SAPK2 phosphorylates cyclin D3 at Thr-283 and targets it for proteasomal degradation.
SIGNOR-280023
P19784
P55087
1
phosphorylation
down-regulates activity
0.338
We found that the stress-induced kinase casein kinase (CK)II phosphorylates the Ser276 immediately preceding the tyrosine motif, increasing AQP4-mu 3A interaction and enhancing AQP4-lysosomal targeting and degradation. | To determine whether Ser276 is an actual CKII substrate, we used GST–AQP4‐Cter proteins in which on...
SIGNOR-250976
Q5VWK5
Q9NPF7
2
binding
up-regulates
0.861
We identify a novel member of the hemopoietin receptor family as a subunit of the receptor for il-23, il-23r.
SIGNOR-87805
Q9Y5G5
Q9Y5I2
2
binding
up-regulates activity
0.2
The clustered protocadherins comprise the largest subfamily of the cadherin superfamily and are predominantly expressed in the nervous system. Pcdh-alpha proteins interact with beta1-integrin to promote cell adhesion. They also form oligomers with Pcdh-gamma proteins at the same membrane sites.
SIGNOR-265702
P27037
Q04771
2
binding
up-regulates
0.664
The major bmp7 type i receptor observed was alk2,
SIGNOR-60234
P06493
Q12834
1
phosphorylation
down-regulates activity
0.935
Cdk1 phosphorylates Cdc20 to negatively regulate its ability to bind and activate the APC/C.
SIGNOR-279321
P19086
Q9UKP6
2
binding
up-regulates activity
0.25
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257319
O75197
P56705
2
binding
up-regulates
0.649
Wnt proteins bind to the frizzled receptors and lrp5/6 co-receptors, and through stabilizing the critical mediator betBeta-catenin, initiate a complex signaling cascade that plays an important role in regulating cell proliferation and differentiation.
SIGNOR-131832
Q9HDB5
Q9NZ94
2
binding
up-regulates activity
0.825
Pre- and postsynaptic plasma membranes are always precisely aligned, and are separated by a synaptic cleft of ~20 nm. The cleft contains an undefined proteinaceous material in the middle, and is presumably bridged by synaptic cell-adhesion molecules such as Nrxns and Nlgns that align the pre- and postsynaptic elements ...
SIGNOR-264167
P17252
Q07954
1
phosphorylation
up-regulates
0.2
Serine and threonine phosphorylation of the low density lipoprotein receptor-related protein by protein kinase calpha regulates endocytosis and association with adaptor moleculesthese results indicate that elimination of serine and threonine phosphorylation sites in the lrp cytoplasmic domain reduces the extent of tyr6...
SIGNOR-127215
P37231
P00519
0
phosphorylation
up-regulates quantity
0.341
We show that the tyrosine kinase Abelson murine leukemia viral oncogene (cAbl) is an adipogenic key regulator. c-Abl promotes adipogenesis by phosphorylation and subsequent stabilization of PPARγ.
SIGNOR-262297
Q14247
P23470
0
dephosphorylation
down-regulates activity
0.2
PTPRG activation by the P1-WD peptide affected the tyrosine phosphorylation of several signaling molecules. Data analysis identified 31 molecules whose phosphorylation was modified in a statistically significant manner (Table I). inhibition of ABL1, BMX, BTK, DAB1, ITGB1, JAK2, KDR, KIT, LIMK1, MET, PDGFRB, SHC1, and V...
SIGNOR-254696
Q99835
P04899
2
binding
up-regulates activity
0.429
it was proposed that Smo might signal through activation of Gi proteins to reduce PKA activity.
SIGNOR-199162
O14773
O76064
0
ubiquitination
up-regulates activity
0.302
Our data demonstrate that RNF8 directly ubiquitylates and stabilizes TPP1 at telomeres.|Taken together, these results suggest that RNF8 dependent K63 linked, but not K48 linked ubiquitin chain formation on TPP1 is required to promote TPP1 stability and function at telomeres.
SIGNOR-278574
O14744
Q15831
0
phosphorylation
up-regulates activity
0.2
We found that PRMT5 is a bona fade substrate for LKB1. We identified T132, 139 and 144 residues, located in the TIM-Barrel domain of PRMT5, as target sites for LKB1 phosphorylation. The point mutation of PRMT5 T139/144 to A139/144 drastically decreased its methyltransferase activity, due probably to the loss of its int...
SIGNOR-277412
P36956
Q5VTR2
0
ubiquitination
down-regulates activity
0.425
Here, we reveal that RNF20-induced SREBP1c ubiquitination down-regulates hepatic lipogenic activity upon PKA activation.|Overexpression of RNF20 represses SREBP1c activity, leading to a decrease in the expression of lipogenic genes.
SIGNOR-278643
P19174
P16234
0
phosphorylation
up-regulates
0.65
Tyrosine phosphorylation has been shown to increase the enzymatic activity of plc-? / we show that the human pdgf ?- And ?-Receptors differ quantitatively in their abilities to associate with and phosphorylate plc-? And to stimulate inositol phosphate production.
SIGNOR-28176
O43353
Q15306
2
binding
down-regulates activity
0.478
These studies demonstrate that inhibition of RICK (RIPK2) polyubiquitination by IRF4 involves polyubiquitination of the kinase domain of RICK and this inhibition is facilitated by binding of IRF4 to the kinase and/or intermediate domains of RICK.
SIGNOR-280454
O95837
P34969
2
binding
up-regulates activity
0.25
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257430
Q13153
P35222
1
phosphorylation
up-regulates
0.557
Pak1 directly phosphorylates _-catenin proteins at ser675 site and this leads to more stable and transcriptional active _-catenin
SIGNOR-175944
Q13490
Q9Y385
1
ubiquitination
down-regulates quantity by destabilization
0.37
We also found that ubiquitin-ligase (E3), c-IAP1 preferentially interacts with phosphorylated Ube2j1. Moreover, we noticed that phosphorylated Ube2j1 is rapidly degraded by the proteasome during ER stress cell recovery. Taken together, these data suggest that Ube2j1 and its phosphorylation is important for transient ER...
SIGNOR-263092
Q9NXA8
O94925
2
binding
up-regulates activity
0.449
Immunoprecipitation assays of interaction between GLS and SIRT5 in HepG2 cells infected with lentivirus containing empty or BAG3 construct. Ectopic BAG3 expression decreases the interaction between GLS and SIRT5. It has been reported that SIRT5 is responsible for desuccinylation of GLS35, immunoprecipitation (IP) was ...
SIGNOR-261207
Q05397
O43707
1
phosphorylation
down-regulates
0.558
Phosphorylation at y12 by fak reduces _-actinin1's affinity for actin [25] and [27]. _-actinin4 is phosphorylated at y4, y31, and y265. Phosphorylation at y4 or y31 decreases its binding to actin [28] while phosphorylation of y265 increases its affinity for actin
SIGNOR-192195
P29597
Q14765
1
phosphorylation
up-regulates activity
0.656
IL-12 activates the Janus family tyrosine kinases JAK2 and Tyk2, which in turn phosphorylate STAT4 on tyrosine 693.
SIGNOR-279303
O15530
P00558
2
phosphorylation
up-regulates activity
0.2
PDPK1 Phosphorylates PGK1 T243. Macrophages increase PGK1 pT243 to reduce the 3-PG affinity of PGK1, which alters the equilibrium of the PGK1-catalyzed reaction toward glycolysis, promoting tumor cell proliferation.
SIGNOR-277402
P60568
Q9UL17
0
transcriptional regulation
down-regulates quantity by repression
0.473
T-bet Transactivates the IFNγ Gene and Represses the IL-2 Gene in EL4 Cells
SIGNOR-266233
P56524
Q5H9F3
2
binding
up-regulates activity
0.427
BCoR-L1 interacts with Class II HDACs, HDAC4, HDAC5, and HDAC7, suggesting that they are involved in its function as transcriptional corepressor.
SIGNOR-259112
P49841
O95863
1
phosphorylation
down-regulates
0.557
Snail is a well-known zn-finger transcription factor that promotes emt by repressing e-cadherin expression. It is known that snail is phosphorylated by gsk3beta and degraded by beta-trcp-mediated ubiquitination. A variant of snail (snail-6sa), which abolishes these phosphorylations, is much more stable and resides excl...
SIGNOR-129402
P00519
Q8WYQ5
1
phosphorylation
up-regulates activity
0.2
The kinase ABL phosphorylates the microprocessor subunit DGCR8 to stimulate primary microRNA processing in response to DNA damage. When coexpressed in HEK293T cells, ABL phosphorylated DGCR8 at Tyr(267).
SIGNOR-262604
P31751
Q99683
1
phosphorylation
down-regulates activity
0.646
Akt2 interacts with and phosphorylates ask1 at ser-83 resulting in inhibition of its kinase activity
SIGNOR-100588
P42338
P49841
1
phosphorylation
down-regulates activity
0.48
In our cultures, both p110alpha and p110beta phosphorylated GSK-3beta at Ser9 confirming previous data.|Whereas p110alpha reduced the protein levels of the CDK2-inhibitor p27 Kip1, p110beta phosphorylated and inactivated GSK-3beta.
SIGNOR-279089
Q9H6T0
Q6ZNA4
0
ubiquitination
up-regulates activity
0.388
These findings suggest that Arkadia ubiquitinates ESRP2 and potentiates the splicing function of ESRP2 ( ).
SIGNOR-278613
P17948
P15692
2
binding
up-regulates
0.843
Vegf exerts its action by binding to vegfr-1 and vegfr-2.
SIGNOR-121132
Q92585
P68431
1
acetylation
down-regulates activity
0.2
The n-terminal domain of maml1 directly interacts with both p300 and histones, and the p300-maml1 complex specifically acetylates histone h3 and h4 tails in chromatin.
SIGNOR-153038
Q16539
P07949
0
phosphorylation
up-regulates
0.332
Dually phosphorylated on thr-180 and tyr-182 by the map2ks map2k3/mkk3, map2k4/mkk4 and map2k6/mkk6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme.
SIGNOR-40493
Q9UPN9
Q15796
2
binding
up-regulates activity
0.638
The ubiquitious nuclear protein transcriptional intermediary factor 1gamma (tif1gamma) selectively binds receptor-phosphorylated smad2/3 in competition with smad4. Rapid and robust binding of tif1gamma to smad2/3 occurs in hematopoietic, mesenchymal, and epithelial cell types in response to tgfbeta. Tif1gamma mediates ...
SIGNOR-236064
P35222
Q05513
0
phosphorylation
down-regulates quantity by destabilization
0.599
 Yap and β-catenin are direct substrates of PKCζ. Similar MS/MS analysis to map the sites phosphorylated in β-catenin by PKCζ identified S45 and several sites of low abundance that included S552 and S675 (Figure S3C).
SIGNOR-276880
P69905
P30519
0
null
down-regulates activity
0.254
Heme oxygenase (HO), by catabolizing heme to bile pigments, down-regulates cellular hemoprotein, hemoglobin, and heme
SIGNOR-251814
Q12933
Q99558
2
binding
down-regulates quantity by destabilization
0.586
We report here that one important mechanism of nik regulation is through its dynamic interaction with the tumor necrosis factor receptor-associated factor 3 (traf3). Traf3 physically associates with nik via a specific sequence motif located in the n-terminal region of nik; this molecular interaction appears to target n...
SIGNOR-124233
P61289
Q9NRC8
2
binding
down-regulates quantity by destabilization
0.2
Here, the authors show that energy stress induces an AMPK-dependent phosphorylation of Sirt7, which promotes its ubiquitin-independent degradation by REGγ, resulting in the down-regulation of rRNA transcription and cell survival.|These results strongly suggest that the phosphorylation status of SirT7 at T153 plays a cr...
SIGNOR-275866
Q9NPG1
P16520
2
binding
up-regulates
0.385
In the non-canonical wnt pathway, frizzled uses galfaq or galfai and gbetagamma dimers to activate phospholipase c (plc), resulting in protein kinase c (pkc) activation and calcium mobilization that regulates the transcription factor nfat, and frizzled also signals through the small gtpases rho and rac to c-jun n-termi...
SIGNOR-152603
O75676
Q16539
0
phosphorylation
up-regulates
0.604
Rskb, a 90-kda ribosomal s6 protein kinase family (rsk) member with two complete catalytic domains connected by a linker, is activated through p38- and erk-mitogen-activated protein kinases. unlike other rsks, the activation loop phosphorylation sites of both catalytic domains of rskb, ser(196) and thr(568), were requ...
SIGNOR-77212
Q15436
Q9NR31
2
binding
up-regulates quantity
0.823
Biogenesis of COPII vesicles is initiated by the activation of the small guanosine triphosphate (GTP)-binding protein secretion-associated Ras-related protein 1 (Sar1) at specialized subdomains of the ER, called ER exit sites (ERES) or transitional ER (tER). Membrane-bound Sar1 then recruits the inner COPII coat subcom...
SIGNOR-265299
Q14457
O96017
0
phosphorylation
up-regulates activity
0.299
We report that CHK2 binds to and phosphorylates Beclin 1 at Ser90/Ser93, thereby impairing Beclin 1-Bcl-2 autophagy-regulatory complex formation in a ROS-dependent fashion.|CHK2 binds to and phosphorylates Beclin 1 at Ser90/Ser93, promoting autophagy via Beclin 1 release from Bcl‐2 sequestration
SIGNOR-264557
P63000
P45984
2
binding
up-regulates
0.523
We show that rac1 activates jnk2 that in turn phosphorylates beta-catenin on critical residues and controls its nuclear translocation.
SIGNOR-178265
Q99558
Q99558
2
phosphorylation
up-regulates activity
0.2
As nik levels increase, nik presumably becomes activated by autophosphorylation (p).
SIGNOR-167063
P19022
O00192
2
binding
up-regulates quantity by stabilization
0.466
To clarify the role of p120 in mammalian cells, we have knocked down p120 with siRNA in cells expressing epithelial (E-), placental (P-), neuronal (N-), and vascular endothelial (VE-) cadherins. We report that each of these cadherins, as well as α- and β-catenins, were rapidly degraded in the absence of p120, resulting...
SIGNOR-252128
P61020
Q99698
2
binding
down-regulates activity
0.2
Mauve interacts with Rab5, Msps, and gamma-tubulin|Mauve/LYST opposes Rab5, which promotes vesicle fusion affecting PCM recruitment
SIGNOR-266002
P49841
P19544
1
phosphorylation
down-regulates quantity by destabilization
0.284
Glycogen synthase kinase 3β promoted phosphorylation of cugWT1 at S64, resulting in ubiquitination and degradation of the cugWT1 associated with the F-box-/- WD repeat-containing protein 8.
SIGNOR-277540
Q05513
O43315
1
phosphorylation
up-regulates
0.2
Wt-pkc_-mediated phosphorylation of wt aqp9 in vitro. In the experiments, substitution of ser11 to ala markedly inhibited phosphorylation. the s11a mutation in fibroblasts caused a smoother cell periphery with fewer aqp9-induced filopodia
SIGNOR-176278
O43524
O43524
2
transcriptional regulation
up-regulates quantity
0.2
We show that FOXO3 binds and activates its own promoter via a positive autoregulatory feedback loop
SIGNOR-255757
Q5S007
P10415
1
phosphorylation
up-regulates activity
0.329
Thus, we propose that Thr56 phosphorylation of Bcl-2 by LRRK2 G2019S might be a crucial step of mitochondrial disorder, which initiates the subsequent cellular damage in the context of PD relevant to G2019S.|We found that LRRK2 G2019S induced loss of the MMP was recovered in both GFP-Bcl-2 and GFP-M-Bcl-2 stable cells ...
SIGNOR-279531
P78536
P01135
1
cleavage
up-regulates activity
0.489
ADAM17 is involved in the release and activation of several growth factors and cytokine receptor ligands. Among the growth factors activated by ADAM17 are TGF-alpha, amphiregulin, epiregulin and HB-EGF
SIGNOR-259841
P42345
P03372
1
phosphorylation
up-regulates activity
0.498
Thus our results indicate that the interaction between ER\u03b1 and raptor is necessary to recruit raptor to the nucleus, where mTOR phosphorylates and activates ER\u03b1.
SIGNOR-278295
Q9P2J5
Q5S007
0
phosphorylation
down-regulates activity
0.2
In this study, we elucidated that leucyl-tRNA synthetase (LRS) was an LRRK2 kinase substrate and identified T293 as an LRRK2 phosphorylation site. LRRK2-meidated LRS phosphorylation or G2019S can lead to impairment of LRS editing, increased ER stress, and accumulation of autophagy markers.
SIGNOR-277417
Q712K3
P68400
0
phosphorylation
up-regulates activity
0.338
Ck2-dependent phosphorylation of the e2 ubiquitin conjugating enzyme ubc3b induces its interaction with beta-trcp and enhances beta-catenin degradation
SIGNOR-88050
P49841
P23246
1
phosphorylation
down-regulates
0.345
Psf is directly phosphorylated by gsk3, thus promoting interaction of psf with trap150, which prevents psf from binding cd45 pre-mrna. / threonine phosphorylation of psf by gsk3 primarily occurs on residue t687
SIGNOR-168392
P30411
P08754
2
binding
up-regulates activity
0.401
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-256838
P13639
Q9H2P9
0
methylation
down-regulates activity
0.747
Analysis of EF2 in the mutant cells revealed a novel form of diphthamide with an additional methyl group that prevented ADP-ribosylation and inactivation of EF2. The abnormal methylation appeared to be catalyzed by DPH5.
SIGNOR-261146
P01112
Q92565
0
guanine nucleotide exchange factor
up-regulates
0.508
Gefs catalyse the transition from gdp-bound, inactive ras to gtp-bound, active ras.
SIGNOR-183732
O14965
Q93073
1
phosphorylation
up-regulates quantity
0.2
To explore the underlying mechanisms, we found that SLAN, a potential tumor suppressor, served as a substrate of Aurora-A and knockdown of SLAN induced immature cytokinesis. Aurora-A phosphorylates SLAN at T573 under the help of the scaffold protein 14-3-3η. Intriguingly, SLAN T573D or T573E inactivated and T573A activ...
SIGNOR-273547
Q92569
Q9H7P9
2
binding
up-regulates activity
0.249
Through deletion and base substitution mutagenesis we have identified Tyr489 of PLEKHG2 as the site phosphorylated by cSrc.The interaction between PLEKHG2 and the full-length of PIK3R3, but not ABL1, occurs in a tyrosine-phosphorylation-dependent manner.
SIGNOR-273809
P37231
Q8IXJ9
2
binding
down-regulates activity
0.2
Our genome-wide analysis confirmed the physiological roles of ASXL1 and ASXL2 in adipogenesis at the molecular level, supporting the hypothesis that ASXL1 is an authentic corepressor of PPARγ, whereas ASXL2 is a PPARγ coactivator, and that together ASXL1 and ASXL2 fine-tune adipogenesis via differential regulation of P...
SIGNOR-260064
P24941
Q12974
0
dephosphorylation
up-regulates
0.2
Cells overexpressing prl-2 exhibited enhanced cyclin-dependent kinase 2 (cdk2) activity
SIGNOR-119478