IdA stringlengths 6 21 | IdB stringlengths 6 21 | labels int64 0 2 | mechanism stringclasses 40
values | effect stringclasses 10
values | score float64 0.1 0.99 ⌀ | sentence stringlengths 10 1.63k ⌀ | signor_id stringlengths 12 14 |
|---|---|---|---|---|---|---|---|
O75197 | P04628 | 2 | binding | up-regulates activity | 0.788 | Ligands such as wnt1, wnt3a, and wnt8 couple the seventransmembrane domain receptor frizzled (fzd) and the single-membrane-spanning low-density receptor-related protein 5/6 (lrp5/6) to activate wnt?Beta-catenin signaling. | SIGNOR-169645 |
O95837 | O43193 | 2 | binding | up-regulates activity | 0.435 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257132 |
Q96CW1 | Q9UI40 | 2 | binding | down-regulates quantity | 0.2 | we report that the first tyrosine motif of NCKX2 interacts with AP2M1 and that the interaction is required for endocytosis of NCKX2 from the dendritic surface. Furthermore, we show that a Src family kinase (SFK) modulates the endocytosis of NCKX2 by tyrosine-phosphorylation of the AP-2 recognition motif in NCKX2. | SIGNOR-264388 |
Q8WU20 | P07949 | 2 | binding | up-regulates | 0.678 | Tyrosine 1062 in ret provides a site for the interaction of multiple signaling molecules and that the balance of shc and snt/frs2 binding may affect the nature of the intracellular signaling for cell proliferation, differentiation and survival induced by activated ret | SIGNOR-108244 |
P43405 | Q06187 | 1 | phosphorylation | up-regulates activity | 0.589 | We have demonstrated that BLNK mediates Syk-dependent Btk activation. In a reconstitution cell system, coexpression of BLNK allows Syk to phosphorylate Btk on its tyrosine 551, leading to the enhancement of Btk activity. | SIGNOR-247586 |
Q16637 | P35637 | 0 | relocalization | up-regulates activity | 0.37 | Here, we report that FUS associates with the SMN complex, mediated by U1 snRNP and by direct interactions between FUS and SMN.|The FUS IP and pulldown revealed that FUS also associates with components of the SMN complex, including SMN and Gemins 4 and 6 |Remarkably, the number of SMN-stained nuclear bodies was dramatic... | SIGNOR-262107 |
P19883 | Q9UJU2 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.28 | We further demonstrate that Fst is a direct target of the WNT/β-catenin pathway. Activation and inactivation of β-catenin induced and inhibited Fst expression, respectively, in both C2C12 cells and mouse embryos. Specific TCF/LEF1 binding sites within the promoter and intron 1 region of the Fst gene were required for R... | SIGNOR-251722 |
P35070 | Q15303 | 2 | binding | up-regulates | 0.723 | For example, betacellulin binds to and activates both erbb1 and erbb4, whereas epiregulin binds to erbb1, erbb3 and erbb4 | SIGNOR-195347 |
Q13489 | Q9NR28 | 2 | ubiquitination | down-regulates quantity by destabilization | 0.792 | Here we show that cIAP1 and cIAP2 are E3 ubiquitin-protein isopeptide ligases (ubiquitin ligases) for Smac. cIAPs stimulate Smac ubiquitination both in vivo and in vitro, leading to Smac degradation. cIAP1 and cIAP2 associate with overlapping but distinct subsets of E2 (ubiquitin carrier protein) ubiquitin-conjugating... | SIGNOR-271391 |
P14859 | P35580 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.2 | Myocyte enhancer factor-2 and serum response factor binding elements regulate fast Myosin heavy chain transcription in vivo. We show that the upstream promoter region of the gene most abundantly expressed in mouse skeletal muscles, IIb MyHC, retains binding activity and transcriptional activation for three positive tra... | SIGNOR-238772 |
P03372 | P31749 | 0 | phosphorylation | up-regulates | 0.765 | Studies using mutants of er-alpha demonstrated that akt increased estrogen receptor activity through the amino-terminal activation function-1 (af-1). Serines s104 s106, s118, and s167 appear to play a role in the activation of er-alpha by akt. | SIGNOR-84963 |
P05109 | Q15109 | 2 | binding | up-regulates activity | 0.317 | RAGE and TLR4 are well-characterized S100A8 and S100A9 receptors and expressed in AML cells Once secreted, S100A8 and S100A9 induce immune and inflammatory responses9 through interaction with receptors such as Toll-like receptor 4 (TLR4), receptor for advanced glycation end-product (RAGE), and CD33 | SIGNOR-261919 |
Q9H1A4 | P06493 | 0 | phosphorylation | up-regulates | 0.592 | Apc activation is thought to depend on apc phosphorylation and cdc20 binding. We have identified 43 phospho_sites on apc of which at least 34 are mitosis specific. Of these, 32 sites are clustered in parts of apc1 and the tetratricopeptide repeat (tpr) subunits cdc27, cdc16, cdc23 and apc7. In vitro, at least 15 of the... | SIGNOR-119705 |
Q15349 | P27361 | 0 | phosphorylation | up-regulates | 0.719 | Several lines of investigation have suggested that rsk is phosphorylated and activated by erk1/2 mapk isoforms | SIGNOR-44949 |
P54868 | Q9NXA8 | 0 | post translational modification | up-regulates activity | 0.343 | We demonstrate that SIRT5 regu-lates succinylation of the rate-limiting ketogenicenzyme 3-hydroxy-3-methylglutaryl-CoA synthase 2(HMGCS2) both in vivo and in vitro.|Succinylation of Lysine Residues within the SubstrateBinding Pocket Inhibits HMGCS2 Activity|Here, we use a label-freequantitative proteomic approach to ch... | SIGNOR-267641 |
Q13501 | O75385 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.567 | ULK1 phosphorylates p62 at the Ser403 site. | SIGNOR-278349 |
Q63HK5 | Q13547 | 1 | relocalization | up-regulates activity | 0.2 | We carried out yeast two-hybrid studies with a PTB domain of FE65, focusing on those genes that might be involved in nuclear signaling, and identified and validated Teashirt proteins as FE65 interacting proteins in neurons. Using reporter systems, we observed that FE65 could simultaneously recruit SET, a component of t... | SIGNOR-264814 |
Q6W2J9 | Q99496 | 2 | binding | up-regulates activity | 0.545 | BcoR and Fbxl10/Jhdm1B are among the most abundant Ring1B/Rnf2 interactors identified with the highest confidence, and their association has been validated by coimmunoprecipitation studies; hence we call this the Fbxl10-BcoR complex. In summary, we have widened the set of multiprotein complexes containing the Polycomb... | SIGNOR-252241 |
P08754 | P32238 | 2 | binding | up-regulates activity | 0.252 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257148 |
Q05655 | O00165 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.2 | FBXO25 encodes an orphan F-box protein that determines the substrate specificity of the SCF (SKP1-CUL1-F-box)(FBXO25) ubiquitin ligase complex. An unbiased screen uncovered the prosurvival protein HCLS1-associated protein X-1 (HAX-1) as the bona fide substrate of FBXO25 that is targeted after apoptotic stresses. Protei... | SIGNOR-275562 |
Q00535 | Q86YT6 | 1 | phosphorylation | up-regulates activity | 0.325 | Similar to the mechanism of PAR-1 regulation of Mib in neurogenesis, CDK5 phosphorylation is proposed to enhance Mib1 ligase activity leading to destabilization.|The cyclin dependent kinase 5 (CDK5) enriched in neurons phosphorylates Mib1 and suppresses the inhibitory effects of Mib1 on neurite morphology. | SIGNOR-280217 |
Q9C0K0 | Q9UPW6 | 0 | transcriptional regulation | down-regulates quantity | 0.495 | Satb2 represses the transcription of Nr4a2. The misexpression of Nr4a2 together with Ctip2 induces expression of SubC-specific genes in wild-type Rsp, and simultaneous knockdown of these two genes in Rsp Satb2-mutant cells prevents their fate transition to SubC identity. Thus, Satb2 serves as a determinant gene in the ... | SIGNOR-268931 |
P04637 | Q13625 | 2 | binding | up-regulates | 0.898 | 53bp2 interacts with the tumour suppressor p53 and enhances p53-mediated activation of transcription, possibly by facilitating the dephosphorylation of one or more sites on p53 | SIGNOR-114762 |
Q13547 | Q63HK5 | 0 | relocalization | up-regulates activity | 0.2 | We carried out yeast two-hybrid studies with a PTB domain of FE65, focusing on those genes that might be involved in nuclear signaling, and identified and validated Teashirt proteins as FE65 interacting proteins in neurons. Using reporter systems, we observed that FE65 could simultaneously recruit SET, a component of t... | SIGNOR-264814 |
P53350 | Q38SD2 | 1 | phosphorylation | up-regulates activity | 0.345 | Here we show that LRRK1 is a PLK1 substrate that is phosphorylated on Ser 1790. PLK1 phosphorylation is required for CDK1-mediated activation of LRRK1 at the centrosomes | SIGNOR-275467 |
Q86YJ5 | Q9UHN6 | 1 | ubiquitination | down-regulates quantity by destabilization | 0.2 | MARCH9, a member of the RING-CH family of transmembrane E3 ubiquitin ligases, down-regulates CD4, major histocompatibility complex-I (MHC), and ICAM-1 in lymphoid cells. To identify novel MARCH9 substrates, we used high throughput flow cytometry and quantitative mass spectrometry by stable isotope labeling by amino aci... | SIGNOR-271533 |
P35568 | Q12923 | 0 | dephosphorylation | down-regulates activity | 0.466 | Finally, we report that PTPL1 expression is sufficient to block the IRS-1/phosphatidylinositol 3-kinase/Akt signaling pathway, to inhibit the insulin-like growth factor-I effect on cell survival, and to induce apoptosis.|We first show by complementary approaches that PTPL1 specifically dephosphorylates insulin receptor... | SIGNOR-277053 |
Q7KZI7 | Q9BXM7 | 1 | phosphorylation | up-regulates activity | 0.367 | MARK2 phosphorylated and activated the cleaved form of PINK1 (DeltaN-PINK1 | SIGNOR-278975 |
Q9HC35 | Q9HC98 | 0 | phosphorylation | up-regulates activity | 0.253 | The mitotic kinases NEK6 and NEK7 phosphorylated the EML4 N-terminal domain at Ser144 and Ser146 in vitro, and depletion of these kinases in cells led to increased EML4 binding to microtubules in mitosis. An S144A-S146A double mutant not only bound inappropriately to mitotic microtubules but also increased their stabil... | SIGNOR-273883 |
Q5TEC6 | Q9Y4C1 | 0 | demethylation | up-regulates activity | 0.2 | Using a biochemical assay coupled with chromatography, we have purified a JmjC domain-containing protein, JHDM2A, which specifically demethylates mono- and dimethyl-H3K9. | SIGNOR-276843 |
O15033 | O43236 | 1 | ubiquitination | down-regulates quantity by destabilization | 0.2 | Furthermore, the ubiquitination and degradation of SMAC, HtrA2, and ARTS were significantly enhanced in AREL1-expressing cells following apoptotic stimulation, indicating that AREL1 binds to and ubiquitinates cytosolic but not mitochondria-associated forms of IAP antagonists | SIGNOR-267670 |
P06737 | Q16816 | 0 | phosphorylation | up-regulates activity | 0.54 | It is well-characterized that GP is activated by PhK-mediated serine phosphorylation at Ser-15 | SIGNOR-267399 |
P46108 | Q13905 | 2 | binding | up-regulates | 0.901 | The endogenous c3g could be coprecipitated with crk from cell lysates of cells expressing high levels of c-crk or v-crk, suggesting high binding affinity and a possible interaction in vivo. | SIGNOR-33732 |
P60174 | P12931 | 0 | phosphorylation | up-regulates quantity by stabilization | 0.2 | As shown in xref , Tim was phosphorylated by both Hck and c-Src, and to a lesser extent by Fyn and c-Yes.|In the case of Hck and Fyn, co-expression led to enhanced Tim turnover, while c-Src and c-Yes promoted Tim stability. | SIGNOR-280136 |
Q92847 | O95837 | 2 | binding | up-regulates activity | 0.435 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257429 |
O15516 | P04150 | 1 | transcriptional regulation | down-regulates quantity by repression | 0.41 | We recently reported that the basic helix-loop- helix transcription factor Clock, which is a histone acetyltransferase and a central component of the self-oscillating transcription factor loop that generates circadian rhythms, represses GR transcriptional activity by acetylating lysine residues within the 'lysine clust... | SIGNOR-253699 |
P14635 | O14965 | 0 | phosphorylation | up-regulates activity | 0.576 | A second wave of Cyclin B1-CDK1 phosphorylation by AurA occurs in late prophase.|Simultaneously, AurA activates and targets the Cyclin B1-CDK1 complex at centrosomes [ xref ]. | SIGNOR-280186 |
Q12778 | Q8IW41 | 0 | phosphorylation | up-regulates activity | 0.422 | The kinase MK5 phosphorylates and activates Foxo1 at serine 215, and this modification is required for Foxo1 to induce Rag transcription. | SIGNOR-280037 |
P43403 | P06241 | 0 | phosphorylation | up-regulates activity | 0.574 | Subsequently, Lck and Fyn phosphorylate and activate the Syk family kinase ZAP-70 when it is recruited to the phosphorylated ITAM motifs xref . | SIGNOR-279043 |
Q13546 | Q96AX9 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.295 | These data suggest that after binding, MIB2 inhibits RIPK1 through a mechanism that is dependent on the E3 ligase activity of MIB2.|Whereas MIB2 readily ubiquitylated wild-type RIPK1, mutating K377 to R significantly reduced MIB2 mediated ubiquitylation of RIPK1 (XREF_SUPPLEMENTARY A). | SIGNOR-278633 |
Q6JBY9 | O15264 | 0 | phosphorylation | down-regulates activity | 0.419 | CapZIP was also phosphorylated rapidly by SAPK3/p38γ and SAPK4/p38δ, and even faster and more extensively by JNK1α1, these protein kinases phosphorylating CapZIP in vitro to >3, approx. 2 and >5 mol of phosphate/mol of protein respectively within a few minutes. Following tryptic digestion and C18 chromatography, furthe... | SIGNOR-263084 |
P69892 | Q9H165 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.446 | Our findings reveal that direct γ-globin gene promoter repression by BCL11A underlies hemoglobin switching. | SIGNOR-269066 |
Q6ZNA4 | P29590 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.355 | Upon TGF-β induction, interaction of Arkadia with phosphorylated Smad2 triggers degradation of SnoN, whereas upon arsenic treatment, interaction of Arkadia with poly-SUMO in PML nuclear bodies induces degradation of polysumoylated PML together with RNF4. | SIGNOR-272883 |
Q9NT62 | P60520 | 2 | binding | up-regulates activity | 0.873 | Three human atg8 (hatg8) homologs, lc3, gabarap, and gate-16, have been characterized as modifiers in reactions mediated by hatg7 (an e1-like enzyme) and hatg3 (an e2-like enzyme) | SIGNOR-141926 |
P24941 | P29350 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.287 | Interaction between SHP-1 and Cdk2 was confirmed by co-immunoprecipitations whereby co-precipitated Cdk2 phosphorylated SHP-1 protein. | SIGNOR-279147 |
Q9UJZ1 | P07766 | 2 | binding | up-regulates activity | 0.2 | We observed that SLP-2 steadily associated with the CD3-epsilon chain of the TCR complex under resting conditions and during the 60 min of stimulation|The SLP-2-associated pool of these molecules became phosphorylated/activated in a sequential manner, a profile compatible with their temporal involvement in early TCR si... | SIGNOR-260375 |
Q14332 | O75197 | 2 | binding | up-regulates activity | 0.688 | Here we report that Wnt receptor Frizzled (Frz) and theco-receptors LRP5 and LRP6 (LRP5/6) directly interact with each other and this interaction is regulated by the LRP6 ectodomain. | SIGNOR-258968 |
P36873 | O75807 | 2 | binding | up-regulates activity | 0.698 | Dephosphorylation of eIF2α is central to ISR signal termination to restore protein synthesis and normal cell functioning 15. It is mediated by protein phosphatase 1 (PP1) complex that recruits a PP1 catalytic subunit (PP1c) and one of the two regulatory subunits. In mammals, phosphatase activity is regulated by either ... | SIGNOR-260174 |
P12931 | Q14790 | 1 | phosphorylation | down-regulates | 0.453 | Src kinase phosphorylates caspase-8 on tyr380: a novel mechanism of apoptosis suppressionwe identified caspase-8 as a new substrate for src kinase. Phosphorylation occurs on tyr380, situated in the linker region between the large and the small subunits of human procaspase-8, and results in downregulation of caspase-8 p... | SIGNOR-146127 |
Q9UGK3 | P12931 | 0 | phosphorylation | up-regulates activity | 0.406 | To examine this possibility, STAP-2 was co-transfected with constitutively active tyrosine kinases in HEK-293 cells. STAP-2 was strongly phosphorylated by various tyrosine kinases, including v-Src (Fig.2 A-a), a JAK2 tyrosine kinase Tyr-22 and Tyr-322 are the major tyrosine phosphorylation sites by v-Src. | SIGNOR-247337 |
O43597 | P46934 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.39 | Endogenous and overexpressed Nedd4 polyubiquitinate Spry2 via Lys(48) on ubiquitin and decrease its stability. | SIGNOR-271425 |
P49841 | P06401 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.277 | Here, we have found that glycogen synthase kinase (GSK)-3β phosphorylation of progesterone receptor-A (PR-A) facilitates its ubiquitination. GSK-3β-mediated phosphorylation of serine 390 in PR-A regulates its subsequent ubiquitination and protein stability. | SIGNOR-276498 |
P46531 | Q16665 | 1 | relocalization | up-regulates activity | 0.649 | The notch intracellular domain interacts with hif-1alpha and hif-1alpha is recruited to notch-responsive promoters upon notch activation under hypoxic conditions. | SIGNOR-141315 |
P98155 | P78509 | 2 | binding | up-regulates | 0.791 | The hypothesis that the vldl receptor signals in response to reelin binding was recently supported by studies (37) showing direct binding of reelin to the vldl receptor and changes in tyrosine phosphorylation in response to reelin-vldl receptor association. | SIGNOR-106295 |
O00398 | O95837 | 2 | binding | up-regulates activity | 0.385 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257213 |
P31751 | P62714 | 0 | dephosphorylation | down-regulates | 0.481 | These results confirm that the activity changes observed are achieved by a reversible phosphorylation mechanism, and also argue that pp2a may negatively regulate rac-pk activity in vivo. Dephosphorylation of the activated rac-pk in itro by pp2ac resulted in an 87% reduction of kinase activity | SIGNOR-42123 |
P28482 | O60716 | 1 | phosphorylation | down-regulates activity | 0.27 | Upon TGFβ treatment, activated extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylates T900 of p120-catenin to promote its interaction with Smurf1 and subsequent monoubiquitination. TGFβ promotes monoubiquitination of p120-catenin through Smurf1 to induce junction dissociation. | SIGNOR-277505 |
P36894 | Q99717 | 1 | phosphorylation | up-regulates activity | 0.688 | Two types of bmp-induced signaling pathways are known, the smad and p38 mapk pathways. In the former case, bmpr1 phosphorylates smad-1,-5,-8, which forms a complex with smad4 that translocates into the nucleus and regulates gene expression. | SIGNOR-255261 |
P22888 | P10588 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.302 | Functional analysis showed that EAR2 and EAR3/COUP-TFI repressed the hLHR promoter activity, whereas TR4 activated hLHR gene transcription. | SIGNOR-266216 |
Q8N122 | Q13131 | 0 | phosphorylation | down-regulates activity | 0.687 | Ampk in turn inactivates mtorc1 directly by phosphorylating raptor and indirectly by phosphorylating tsc2. | SIGNOR-173035 |
Q6UW88 | Q15303 | 2 | binding | up-regulates | 0.394 | Areg (amphiregulin), btc (beta-cellulin), egf, epgn (epigen), ereg (epiregulin), hbegf, nrg1, nrg2, nrg3, nrg4 and tgfa (tgfalpha) constitute egf family ligands for erbb family receptors. | SIGNOR-147835 |
Q9BZL6 | P41182 | 1 | phosphorylation | down-regulates activity | 0.2 | Prkd2 directly binds to Bcl6 and Prkd2-dependent phosphorylation of Bcl6 is necessary to constrain Bcl6 to the cytoplasm, thereby limiting TFH development. | SIGNOR-279651 |
Q9HCK8 | P41225 | 1 | transcriptional regulation | down-regulates quantity | 0.2 | Many of the most significantly up-regulated genes in Chd8+/− and Chd8−/− NPCs are involved in later stages of neuronal development, including Ascl1 [a central driver of neural reprogramming (29)], Dcx, Map2, Nefm, Neurod4, and Neurog1 (Fig. 2 E and F). Additionally, we found that Sox3 is derepressed in both Chd8+/− and... | SIGNOR-268920 |
O75197 | O14905 | 2 | binding | up-regulates | 0.595 | Wnt proteins bind to the frizzled receptors and lrp5/6 co-receptors, and through stabilizing the critical mediator betBeta-catenin, initiate a complex signaling cascade that plays an important role in regulating cell proliferation and differentiation. | SIGNOR-132114 |
P43250 | O75581 | 1 | phosphorylation | up-regulates activity | 0.345 | In contrast to the GRK5 and GRK6 stimulated activity of wild-type LRP6, the LRP6 M5 mutant failed to respond to the expression of GRK5 or GRK6 (XREF_FIG C) by increased TOPflash reporter activity, indicating that PPPSP motifs are indispensable for GRK5- and GRK6 mediated LRP6 activation.|Our findings that GRK5 and GRK6... | SIGNOR-279412 |
P68363 | Q9BYW2 | 0 | methylation | up-regulates activity | 0.244 | The histone methyltransferase SET-domain-containing 2 (SETD2), which is responsible for H3 lysine 36 trimethylation (H3K36me3) of histones, also methylates α-tubulin at lysine 40, the same lysine that is marked by acetylation on microtubules. Methylation of microtubules occurs during mitosis and cytokinesis and can be ... | SIGNOR-269090 |
O60674 | Q14457 | 1 | phosphorylation | up-regulates activity | 0.297 | Mechanistically, IL-6 triggers the interaction between JAK2 and BECN1, where JAK2 phosphorylates BECN1 at Y333. We demonstrate that BECN1 Y333 phosphorylation is crucial for BECN1 activation and IL-6-induced autophagy by regulating PI3KC3 complex formation. | SIGNOR-277567 |
P17612 | P52888 | 1 | phosphorylation | up-regulates activity | 0.307 | PKA phosphorylation is suggested to play a regulatory role in EP24.15 enzyme activity. Mutation analysis of each putative PKA site, in vitro phosphorylation, and phosphopeptide mapping indicated serine 644 as the phosphorylation site. The most dramatic change upon PKA phosphorylation was a substrate-specific, 7-fold in... | SIGNOR-250060 |
P35372 | Q9UQM7 | 0 | phosphorylation | down-regulates | 0.2 | The decrease in mu-opioid receptor activity after chronic agonist exposure (1 microm [d-ala(2),n-mephe(4),gly-ol(5)]-enkephalin) is largely due to kinase-mediated phosphorylation of intracellular receptor domains. We have recently shown that the substitution of two putative ca(2+)/calmodulin-dependent protein kinase ii... | SIGNOR-79682 |
P19021 | P01178-PRO_0000020495 | 1 | cleavage | up-regulates activity | 0.2 | Nevertheless, overall the results of this study show that peptide sequence recognition is an important aspect of the interactions of the prohormone substrates prooxytocin (3d) and procalcitonin (7e) with PAM, which is mirrored in the potency of analogous peptidomimetic glycolate inhibitors of the enzyme. | SIGNOR-268551 |
Q99496 | Q8NHM5 | 2 | binding | up-regulates activity | 0.609 | BcoR and Fbxl10/Jhdm1B are among the most abundant Ring1B/Rnf2 interactors identified with the highest confidence, and their association has been validated by coimmunoprecipitation studies; hence we call this the Fbxl10-BcoR complex. The assembly of Fbxl10-BcoR complex(es), the associations among its various subunits, ... | SIGNOR-252242 |
O14558 | P17612 | 0 | phosphorylation | down-regulates | 0.2 | Hosphorylation of hsp20 at ser16 is not only associated with cyclic nucleotide-dependent vasorelaxation but also inhibits agonist-induced contractile responses. | SIGNOR-66493 |
Q16549 | P49715 | 1 | phosphorylation | down-regulates | 0.2 | Addition of active p38a induced phosphorylation of wild-type c/ebp_? (c/ebp_?WT) on serine 21 | SIGNOR-186202 |
Q15633 | Q9UPY3 | 2 | binding | up-regulates | 0.94 | Dicer and trbp interact in vivo and in vitro /our data indicate that trbp is primarily required for the assembly and/or functioning of si? Or mi?RISCs In mammalian cells, but it may also facilitate the cleavage of pre?miRNAs By dicer. | SIGNOR-140226 |
Q5JU85 | P42262 | 1 | relocalization | up-regulates quantity | 0.2 | BRAG1 increases the synaptic recycling pool of AMPARs.these data suggest that the BRAG1 enhancement of AMPAR transmission is mediated by the increased expression of the recycling pool of synaptic GluA2/3 receptors. | SIGNOR-264913 |
O95831 | Q9P286 | 0 | phosphorylation | down-regulates activity | 0.2 | Our results show that PAK5 can phosphorylate Thr-281 of AIF, which is included in its NLS1 sequence.|These results suggested that PAK5 inhibited AIF from entering the nucleus through phosphorylation of AIF T281 site, thus inhibiting cell apoptosis. | SIGNOR-279085 |
O75037 | Q9BZY9 | 2 | binding | up-regulates activity | 0.2 | Here we show that the ubiquitin E3 ligase TRIM3, also known as BERP, interacts with KIF21B via its RBCC domain ||he E3-ligase function of TRIM3 is not involved in KIF21B degradation, however TRIM3 depletion reduces the motility of the motor. Together, our data suggest that TRIM3 is a regulator in the modulation of KIF2... | SIGNOR-272479 |
P29597 | P18031 | 0 | dephosphorylation | down-regulates activity | 0.646 | Upon ligand binding, IL-2R , IL-6R or LeptinR , IFN-_R , IFN-_R and PRLR or growth hormone (GH) receptor associated JAKs become activated. These JAKs mediate phosphorylation of specific tyrosine residues and recruit STATs. Activated STATs are released from the receptor and translocate to the nucleus. PTP1B dephosphory... | SIGNOR-134564 |
P38398 | O96017 | 0 | phosphorylation | up-regulates | 0.787 | In this study, we tested the hypothesis that the brca1-mediated regulation of recombination requires the chk2- and atm-dependent phosphorylation sites. | SIGNOR-120575 |
A6NLP5 | P32754 | 2 | binding | up-regulates quantity by stabilization | 0.27 | Decreased expression of 4-hydroxyphenylpyruvic acid dioxygenase (HPD), a key enzyme for tyrosine metabolism, is a cause of human tyrosinemia. However, the regulation of HPD expression remains largely unknown. Here, we demonstrate that molecular chaperone TTC36, which is highly expressed in liver, is associated with HPD... | SIGNOR-272960 |
P41229 | Q16695 | 1 | demethylation | up-regulates activity | 0.2 | KDM5 subfamily is capable of removing tri†and di†methyl marks from lysine 4 on histone H3 (H3K4). Depending on the methylation site, its effect on transcription can be either activating or repressing. | SIGNOR-264306 |
P51608 | P16220 | 1 | post transcriptional regulation | up-regulates quantity by expression | 0.531 | Interestingly, Creb1 was one of the activated MeCP2 targets that we validated by quantitative real-time RT-PCR (Fig. 1C), and using ChIP analysis we found that in vivo MeCP2 binds to the promoter region of Creb1, with significantly enhanced binding in MECP2-Tg samples compared to WT (p < 0.05) | In addition, Sst and CR... | SIGNOR-264682 |
Q9BZS1 | P06239 | 0 | phosphorylation | down-regulates | 0.401 | Lck phosphorylated tyr-342 of foxp3 by immunoprecipitation and in vitro kinase assay, and the replacement of tyr-342 with phenylalanine (y342f) abolished the ability to suppress mmp9 expression. | SIGNOR-203089 |
P38405 | P30518 | 2 | binding | up-regulates activity | 0.269 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256897 |
P05412 | P27635 | 2 | binding | down-regulates | 0.387 | The qm gene encodes a 24.5 kda ribosomal protein l10 known to be highly homologous to a jun-binding protein (jif-1), which inhibits the formation of jun-jun dimers. | SIGNOR-90750 |
P19784 | Q92598 | 1 | phosphorylation | down-regulates activity | 0.327 | Protein kinase CK2 phosphorylates Hsp105 alpha at Ser509 and modulates its function. | the phosphorylation of Hsp105 alpha at Ser(509) abolished the inhibitory activity of Hsp105 alpha in vitro. | SIGNOR-251008 |
P30530 | Q63HR2 | 1 | phosphorylation | up-regulates quantity | 0.2 | In this study, however, we demonstrate for the first time that Axl directly binds to and phosphorylates TNS2 at Y483.|Overall these results suggest that Axl positively regulates TNS2 expression. | SIGNOR-278471 |
P63000 | Q13459 | 0 | gtpase-activating protein | down-regulates activity | 0.541 | We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity... | SIGNOR-260510 |
P45983 | P38936 | 1 | phosphorylation | up-regulates quantity by stabilization | 0.672 | The stress-activated protein kinases p38 alpha and jnk1 stabilize p21(cip1) by phosphorylation.|p38 alpha and JNK1 phosphorylated p21 in vivo, and both p38 alpha and JNK1 phosphorylated p21 at Ser(130) in vitro. | SIGNOR-89440 |
P43088 | P38405 | 2 | binding | up-regulates activity | 0.25 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256953 |
P12830 | Q12955 | 2 | binding | up-regulates activity | 0.323 | Ankyrin-G is a molecular partner of E-cadherin in epithelial cells and early embryos. Ankyrin-G also recruits beta-2-spectrin to E-cadherin-beta-catenin complexes, thus providing a direct connection between E-cadherin and the spectrin/actin skeleton. | SIGNOR-266710 |
P63000 | Q9NRY4 | 0 | gtpase-activating protein | down-regulates activity | 0.724 | We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity... | SIGNOR-260493 |
Q08209 | P0DP23 | 2 | binding | up-regulates | 0.751 | Calcium-bound calmodulin associates with calcineurin (cn), releasing the phosphatase from the repressive effects on an autoinhibitory domain. | SIGNOR-114098 |
P23443 | Q9HC98 | 0 | phosphorylation | up-regulates activity | 0.367 | Here we demonstrate that in addition to phosphorylating S6K1 and SGK1 at their hydrophobic motif, NEK6 also phosphorylates S6K1 at two other sites and phosphorylates SGK1 at one other site in vitro. Analysis of the peptides phosphorylated by NEK6 (Fig 2), performed in the present study has confirmed this, and identifie... | SIGNOR-262953 |
P06493 | Q9NZH5 | 1 | phosphorylation | up-regulates activity | 0.291 | HPTTG is phosphorylated by Cdc2 at Ser165. we show that hPTTG is phosphorylated during mitosis. The direct phosphorylation of hPTTG by Cdc2 is interesting in itself since the substrates of this master mitotic kinase are supposed to play important roles in the initiation and progression of mitosis. | SIGNOR-262700 |
Q14451 | P06213 | 2 | binding | up-regulates | 0.418 | Insulin induces the ir-grb7 interaction in cells expressing physiological levels of the proteins, suggesting that grb7 is implicated in insulin signaling. | SIGNOR-77153 |
Q03591 | P01024 | 2 | binding | up-regulates activity | 0.833 | Finally, we have been able to establish that CFHR1 can sterically inhibit the interaction that CFH/CFHL-1 SCR1-4 makes with C3b.|CFH regulates the alternative pathway of complement in both the fluid phase and on self-surfaces: It competes with complement factor B (CFB) for binding to C3b and C3(H2O) thereby blocking th... | SIGNOR-263475 |
P06241 | Q12879 | 1 | phosphorylation | up-regulates activity | 0.734 | To gain further insight into the roles of Src and Fyn in the phosphorylation and regulation of the NMDA receptor, we have characterized the tyrosine phosphorylation of NR2A and NR2B by exogenous Src and FynIn the case of NR2A, three potential tyrosine phosphorylation sites have been proposed: Tyr1105, Tyr1267 and Tyr13... | SIGNOR-247151 |
Q92796 | Q8NFP9 | 2 | binding | up-regulates activity | 0.42 | Interestingly, a recent proteomic analysis (Lauks et al., 2012) revealed that Nbea binds SAP102, which interacts with glutamate receptors early in the biosynthetic route and regulates their targeting. This finding, along with the interaction of Nbea with the glycine receptor beta subunit (del Pino et al., 2011), furthe... | SIGNOR-266004 |
Q99442 | Q9UGP8 | 2 | binding | up-regulates activity | 0.96 | Sec63 was identified as a novel substrate and binding partner of protein kinase CK2. We identified serine 574, serine 576 and serine 748 as CK2 phosphorylation sites. Phosphorylation of Sec63 by CK2 enhanced its binding to Sec62. | SIGNOR-265273 |
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