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14
P27708
P28482
0
phosphorylation
up-regulates
0.382
Cad is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. The activation of the pathway required for cell proliferation is a consequence of the phosphorylation of cad thr-456 by mitogen-activated protein (map) kinase.Activated map kinase (erk1/2), the enzyme responsible fo...
SIGNOR-137171
Q92974
P27448
0
phosphorylation
up-regulates activity
0.382
Rho-Rac guanine nucleotide exchange factor 2 (ARHGEF2), which activates Ras homolog family member A (RHOA), is anchored to the microtubule network and sequestered in an inhibited state through binding to dynein light chain Tctex-1 type 1 (DYNLT1). We showed in mammalian cells that liver kinase B1 (LKB1) activated the m...
SIGNOR-277368
P0DP25
P00533
0
phosphorylation
down-regulates
0.382
Phosphorylation of calmodulin by the epidermal-growth-factor-receptor tyrosine kinase. Phosphorylated calmodulin does not exhibit the characteristic ca2+ shift normally observed with calmodulin in electrophoretic gels, an observation that is consistent with this modification affecting the biological activity of the mol...
SIGNOR-266335
Q13153
O95747
0
phosphorylation
down-regulates activity
0.382
OSR1 phosphorylated threonine 84 in the N-terminal regulatory domain of PAK1. phosphorylation of PAK1 by OSR1 desensitizes PAK1 to activation by small G proteins, providing a modulatory input to PAK1 activity.
SIGNOR-250210
P06730
P17252
0
phosphorylation
up-regulates
0.382
Phosphorylation of eIF-4E on serine 209 by protein kinase C is inhibited by the translational repressors, 4E-binding proteins.[..] This suggests a two-step model for the phosphorylation (and activation) of eIF4E by growth factors and hormones: first, dissociation of eIF4E .
SIGNOR-248945
P01116
P49354
0
null
up-regulates activity
0.382
Major investments have been made to target Ras through indirect routes. Inhibition of farnesyl transferase to block Ras maturation has failed in large clinical trials.
SIGNOR-242559
P31645
Q13976
0
phosphorylation
up-regulates
0.382
These results are consistent with the hypothesis that pkg phosphorylates hsert at thr-276 and increases its activity by modifying the substrate permeation pathway formed, in part, by tm5.
SIGNOR-158186
O75581
P45983
0
phosphorylation
up-regulates
0.382
We show that several proline-directed mitogen-activated protein kinases (mapks), such as p38, erk1/2, and jnk1 are sufficient and required for the phosphorylation of ppps/tp motifs of lrp6. External stimuli, which control the activity of mapks, such as phorbol esters and fibroblast growth factor 2 (fgf2) control the ch...
SIGNOR-169007
P35052
O75093
0
binding
up-regulates
0.382
Slit family proteins are functional ligands of glypican-1 in nervous tissue and suggest that their interactions may be critical for certain stages of central nervous system histogenesis.
SIGNOR-68327
P33981
P53350
0
phosphorylation
up-regulates activity
0.381
Here, we demonstrate that Plk1 promotes checkpoint signaling at kinetochores through the phosphorylation of at least two Mps1 substrates, including KNL-1 and Mps1 itself. As a result, Plk1 activity enhances Mps1 catalytic activity as well as the recruitment of the SAC components Mad1:C-Mad2 and Bub3:BubR1 to kinetochor...
SIGNOR-276199
Q8NEA6
Q9UMX1
0
binding
down-regulates
0.381
These data indicate that the inhibition of glis3-mediated transactivation by sufu appears to rely on the interaction with glis3 through the ygh motif and is not related to an effect on the general transcriptional machinery
SIGNOR-173573
P38432
P24941
0
phosphorylation
up-regulates
0.381
In particular, we have recently found that the cdk2/cyclin e complex can phosphorylate coilin in vitro . there is but a single consensus cdk2/cyclin e phosphorylation site in coilin, located at serine 184. when serine 184 was mutated to an alanine (s184a), mimicking a dephosphorylated state, a nucleolar mislocalization...
SIGNOR-84949
O15169
P63092
0
binding
up-regulates
0.381
We show that pge2 stimulates colon cancer cell growth through its heterotrimeric guanine nucleotide-binding protein (g protein) coupled receptor, ep2, by a signaling route that involves the activation of phosphoinositide 3-kinase and the protein kinase akt by free g protein bg subunits and the direct association of the...
SIGNOR-141789
P12830
P56545
0
transcriptional regulation
down-regulates quantity by repression
0.381
Overexpression of the CtBP2 protein enhanced the repression activity of the E-cadherin promoter in a dose-dependent manner, whereas overexpression of ataxin-1 increased the activity of the E-cadherin promoter in a dose-dependent manner 
SIGNOR-261578
P10275
P11309
0
phosphorylation
up-regulates activity
0.381
PIM1 phosphorylates the AR and 14-3-3 ζ and coordinates their interaction. PIM1 phosphorylation of the AR and 14-3-3 ζ enhances their interaction and shifts their occupancy on chromatin, resulting in 14-3-3 ζ co-regulation of AR, likely by recruiting other AR co-regulators such as hnRNPK and TRIM28.
SIGNOR-277575
O60674
Q8NI17
0
binding
up-regulates
0.381
Il-31 can activate janus kinase (jak) 1 and jak2 signaling molecules after binding to its receptor complex.
SIGNOR-161430
P67809
Q7Z6M2
0
binding
down-regulates quantity by destabilization
0.381
Here we identify FBX33 as a component of an SCF E3-ubiquitin ligase that targets the multifunctional regulator Y-box binding protein 1 (YB-1)/dbpB/p50 for polyubiquitination and destruction by the proteasome. By targeting YB-1 for proteasomal degradation, FBX33 negatively interferes with YB-1 mediated functions.  FBX33...
SIGNOR-271604
Q92918
P07948
0
phosphorylation
up-regulates activity
0.381
BCR ligation induced rapid tyrosine-phosphorylation of HPK1 mainly by Syk and Lyn, resulting in its association with BASH and catalytic activation. Tyr-379 within HPK1 is essential for binding to BASH and thus strongly suggest that the DDDYDDV sequence containing the phosphorylated Tyr-379 is the binding site for the B...
SIGNOR-251403
Q9ULX9
P35452
0
binding
down-regulates activity
0.381
Hoxd12 and MHox, that interact with v-/c-Maf, using the phage display method. The Hox proteins also could associate with the other Maf protein family members, MafB, MafK, MafF, and MafG, but not with Jun and Fos. The Hox proteins negatively regulated the DNA binding, transactivation and cell-transforming abilities of M...
SIGNOR-221884
P41180
Q9NV58
0
ubiquitination
down-regulates quantity by destabilization
0.381
Coexpression with dorfin decreased the amount of total CaR protein and increased CaR ubiquitination, whereas a dominant negative fragment of dorfin had opposite effects. dorfin-mediated proteasomal degradation of immature CaR occurs from the endoplasmic reticulum. Because endogenous CaR in Madin-Darby canine kidney cel...
SIGNOR-271456
P28069
Q92793
0
binding
up-regulates activity
0.381
 We and others have recently shown that CBP can constitutively bind to Pit-1 and synergistically activate transcription of promoters containing Pit-1 DNA-binding sites.
SIGNOR-267204
Q15208
Q9Y6E0
0
phosphorylation
up-regulates
0.381
Ndr1/ndr2 protein kinase is activated by phosphorylation on the activation loop phosphorylation site ser281/ser282 and the hydrophobic motif phosphorylation site thr444/thr442. Autophosphorylation of ndr is responsible for phosphorylation on ser281/ser282, whereas thr444/thr442 is targeted by an upstream kinase. Here w...
SIGNOR-142467
Q9BT67
Q9H0M0
0
ubiquitination
down-regulates quantity
0.381
Accordingly, the ubiquitination assays were performed and the results revealed that knockdown of WWP1 reduced the ubiquitination of NDFIP1 in HuCCT1 and vice versa in HCCC-9810 and RBE (Fig. 7E).|In addition, we found that WWP1 could reduce the protein level of NDFIP1 via ubiquitination.
SIGNOR-278796
Q96ST2
Q9Y243
0
phosphorylation
up-regulates activity
0.381
The data presented in this report confirmed the differential phosphorylation of IWS1 at Ser720/Thr721 by Akt3 and Akt1 and showed that its phosphorylation at this site is required for the recruitment of SetD2 to the Spt6-IWS1-Aly/REF complex.
SIGNOR-273496
Q9UBN7
P49841
0
phosphorylation
up-regulates activity
0.381
GSK3beta was found to co-localize with HDAC6 in hippocampal neurons, and inhibition of GSK3beta resulted in decreased binding of antibody to phosphoserine-22, a potential GSK3beta phosphorylation site in HDAC6.|This suggests that GSK3\u03b2 may directly phosphorylate HDAC6 at this site, although further work with purif...
SIGNOR-278941
P06241
Q16620
0
binding
up-regulates
0.381
All these data suggest the involvement of fyn in the neurotrophin signal transduction pathways downstream of trkb. We investigated whether fyn is involved in the trk-dependent signal transduction pathways of neurotrophin. The fyn-src homology domain 2 (sh2) was observed to associate in vitro with the intracellular doma...
SIGNOR-58424
P09917
P28482
0
phosphorylation
up-regulates activity
0.381
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product ...
SIGNOR-264409
Q09472
Q86Y01
0
binding
up-regulates
0.381
We found that a significant fraction of dtx1 proteins were localized in the nucleus and physically interacted with the transcriptional coactivator p300.
SIGNOR-110629
Q16552
Q9BYH8
0
transcriptional regulation
up-regulates quantity by expression
0.381
In cooperation with RORγt and RORα, IκBζ enhances Il17a expression by binding directly to the regulatory region of the Il17a gene. 
SIGNOR-266210
P27986
P36897
0
binding
up-regulates
0.381
These studies revealed that PI 3-kinase is associated in vivo with both TGF-_ receptor subtypes and that TGF-_1 stimulation enhances PI 3-kinase activity associated with type I TGF-_ receptor in hASM cells.
SIGNOR-227525
P30086
P05129
0
phosphorylation
up-regulates activity
0.381
Here we report that one mechanism involves dissociation of Raf kinase inhibitory protein (RKIP) from Raf-1. Classic and atypical but not novel PKC isoforms phosphorylate RKIP at serine 153 (Ser-153). RKIP Ser-153 phosphorylation by PKC either in vitro or in response to 12-O-tetradecanoylphorbol-13-acetate or epidermal ...
SIGNOR-249190
Q96IZ0
P0C2W1
0
binding
down-regulates quantity by destabilization
0.381
Fbxo45 interacts with Par-4 in the cytoplasm and mediates its ubiquitylation and proteasomal degradation. Fbxo45 silencing results in stabilization of Par-4 with increased apoptosis.Fbxo45 forms an atypical ubiquitin ligase complex that contains Skp1 and the Ring-finger protein PAM (protein-associated with myc) also kn...
SIGNOR-272223
Q9NRM7
O14965
0
phosphorylation
up-regulates
0.38
On the basis of these observations, we conclude that s83 of lats2 is a phosphorylation target of aurora-a and this phosphorylation plays a role of the centrosomal localization of lats2.
SIGNOR-124830
O75925
O15111
0
phosphorylation
up-regulates activity
0.38
In addition, we show that IKKalpha is associated with PIAS1 and mediates the S90 phosphorylation of PIAS1.|Mutational studies indicate that Ser90 phosphorylation is required for PIAS1 to repress transcription.
SIGNOR-278926
P60520
Q96A56
0
binding
up-regulates
0.38
In this work, we show that tp53inp1 is also able to interact with atg8-family proteins and to induce autophagy-dependent cell death. mammalian cells contain multiple atg8 orthologs belonging to three subfamilies: microtubule-associated protein 1 light chain 3, -aminobutyric acid receptor-associated protein (gabarap) a...
SIGNOR-196667
P14598
Q9NWZ3
0
phosphorylation
up-regulates
0.38
Phosphorylation of the cytosolic factor p47phox is essential for activation of the nadph oxidase.We found that thr133, ser288 and thr356, targets for irak-4 phosphorylation in vitro, are also phosphorylated in endogenous p47phox after lps stimulation. We conclude that irak-4 phosphorylates p47phox and regulates nadph o...
SIGNOR-152027
Q5EBL8
Q6IQ23
0
binding
up-regulates activity
0.38
Using cell biological and biochemical methods, we now show that ADAM10 is docked to junctions by its transmembrane partner Tspan33, whose cytoplasmic C terminus binds to the WW domain of PLEKHA7 in the presence of PDZD11. The PLEKHA7-PDZD11 Complex Clusters ADAM10 at Junctions through Tspan33
SIGNOR-261253
Q03135
P04150
0
binding
up-regulates
0.38
He mGR appears to reside in caveolae and its association with caveolin-1 (Cav-1) was clearly detected in two of the four cell lines investigated using double recognition proximity ligation assay.
SIGNOR-251683
Q92911
P01222
0
transcriptional regulation
up-regulates quantity by expression
0.38
I– uptake is stimulated by TSH, the master hormone for thyroid gland regulation. TSH stimulation results, at least in part, from the cAMP-mediated increase in NIS biosynthesis. TSH not only stimulates NIS transcription and biosynthesis but is also required for modulating the NIS phosphorylation pattern, maintaining i...
SIGNOR-251995
P53805
P27361
0
phosphorylation
up-regulates activity
0.38
Consensus phosphorylation sites for p42/44 MAPK and GSK-3 are present in the SP repeat of MCIP1 at serine 112 and serine 108, respectively |Several endogenous proteins are capable of inhibiting the catalytic activity of calcineurin. Modulatory calcineurin interacting protein 1 (MCIP1) is unique among these proteins on ...
SIGNOR-249478
Q01860
Q96GD4
0
phosphorylation
down-regulates activity
0.38
Aurkb phosphorylates Oct4(S229) during G2/M phase, leading to the dissociation of Oct4 from chromatin, whereas PP1 binds Oct4 and dephosphorylates Oct4(S229) during M/G1 transition, which resets Oct4-driven transcription for pluripotency and the cell cycle.
SIGNOR-279592
Q16620
P18031
0
dephosphorylation
down-regulates activity
0.38
Collectively, these data establish a direct enzyme-substrate interaction between PTP1B and phosphorylated Y705/706 (p-Y705/706) TRKB, the critical autophosphorylation sites that mediate BDNF-induced signaling.| Therefore, the data are consistent with a role of PTP1B as an inhibitor of BDNF/TRKB signaling
SIGNOR-264554
Q9HBA0
P17252
0
phosphorylation
up-regulates activity
0.38
We conclude that the serine/threonine kinases PKC and PKA enhance activation of the TRPV4 ion channel by phosphorylation at specific sites and that phosphorylation depends on assembly of PKC and PKA by AKAP79 into a signaling complex with TRPV4.
SIGNOR-260882
P18206
P00519
0
phosphorylation
up-regulates activity
0.38
Abl is the tyrosine kinase that phosphorylates vinculin Y822.|Finally we show that Abl inhibition prevents vinculin actions in cadherin containing complexes, resulting in defects in cell stiffening.
SIGNOR-278464
P01023
P14091
0
cleavage
down-regulates quantity by destabilization
0.38
Disruption of structural and functional integrity of alpha 2-macroglobulin by cathepsin E|Analysis of the N-terminal amino-acid sequences of these proteins revealed that alpha 2M was selectively cleaved at the Phe811-Leu812 bond in about 100mer downstream of the bait region.
SIGNOR-266977
Q04759
Q8IVH8
0
phosphorylation
up-regulates
0.38
We report that the kinase glk (map4k3) directly activated pkc-? During tcr signaling.
SIGNOR-176744
Q16832
P12931
0
phosphorylation
up-regulates
0.38
Here, using baculoviral co-expression of the ddr2 cytosolic domain and src, we show that src targets three tyrosine residues (tyr-736, tyr-740, and tyr-741) in the activation loop of ddr2 for phosphorylation. This phosphorylation by src stimulates ddr2 cis-autophosphorylation of additional tyrosine residues.
SIGNOR-140767
Q13480
P18031
0
dephosphorylation
down-regulates activity
0.38
Since Gab1 is negatively regulated by PTP1B, a part of the retinal neuroprotective effect we have observed previously in PTP1B deficient mice could be contributed by Gab1 as well.|The results indicate that PTP1B completely dephosphorylated Gab1 and the mutant protein failed to dephosphorylate Gab1 (Figure\u00a0 xref C)...
SIGNOR-276965
Q96RI1
Q05513
0
phosphorylation
up-regulates
0.38
The effect of fic1 on fxr phosphorylation and nuclear localization and its effects on bsep promoter activity could be blocked with protein kinase c zeta (pkc zeta) inhibitors (pseudosubstrate or small interfering rna silencing). Recombinant pkc zeta directly phosphorylated immunoprecipitated fxr. The mutation of threon...
SIGNOR-179771
Q13322
P06241
0
phosphorylation
down-regulates
0.38
Grb10 tyrosine phosphorylation was stimulated by expression of constitutively active src or fyn in cells and by incubation with purified src or fyn in vitro. The insulin stimulated or src/fyn-mediated tyrosine phosphorylation in vivo was significantly reduced when grb10 tyrosine 67 was changed to glycine. This mutant f...
SIGNOR-78702
Q96N67
Q6ZNJ1
0
binding
up-regulates activity
0.38
In summary, from 129 binding partners of Nbeal2 identified by mass spectrometry, we have confirmed the interaction of 3, Dock7, Sec16a, and Vac14, by different biochemical and cellular approaches|Given the significant reduction of Dock7 levels and its altered localization in Nbeal2−/− platelets, we postulated that this...
SIGNOR-261891
Q01860
P28482
0
phosphorylation
down-regulates
0.38
We demonstrate that oct4a interacts with erk1/2 by using both in vitro gst pulldown and in vivo co-immunoprecipitation assays. Ms analysis identified phosphorylation of oct4a at ser-111. / serine 111 phosphorylation regulates oct4a protein subcellular distribution and degradation.
SIGNOR-192097
Q16143
Q9H4B4
0
phosphorylation
down-regulates activity
0.38
Polo-like kinase (plk) family (plk1, plk2, and plk3) phosphorylate alpha-syn and beta-syn specifically at ser-129 and ser-118, respectively. Polo-like kinase 2 (plk2) phosphorylates alpha-synuclein at serine 129 in central nervous system. The membrane association of pd-linked mutant alpha -synuclein, but not wild-type ...
SIGNOR-189057
P46531
Q13950
0
binding
down-regulates
0.38
Runx2 is an inhibitor of the notch1 signaling pathway during normal osteoblast differentiation. The n-terminal domain of runx2 was crucial to the binding and inhibition of the the n-terminus of the notch1 intracellular domain.
SIGNOR-167627
P63000
Q9Y3L3
0
gtpase-activating protein
down-regulates activity
0.38
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity...
SIGNOR-260514
Q9P1Z0
Q9H2X6
0
phosphorylation
down-regulates activity
0.379
The human protein kinase HIPK2 phosphorylates and downregulates the methyl-binding transcription factor ZBTB4.
SIGNOR-262882
P52655
P68400
0
phosphorylation
up-regulates activity
0.379
We now show that human TFIIA is phosphorylated in vivo on serine residues that are partially conserved between yeast and human TFIIA large subunits. Alanine substitution mutation of serine residues 316 and 321 in TFIIA alphabeta reduced TFIIA phosphorylation significantly in vivo. Additional alanine substitutions at se...
SIGNOR-250877
P45983
P14778
0
phosphorylation
up-regulates activity
0.379
Il-1 binding to its receptor triggers a cascade of signaling events, including activation of the stress-activated mitogen-activated protein (map) kinases, c-jun nh2-terminal kinase (jnk) and p38 map kinase, as well as transcription factor nuclear factor kappab (nf-kappab
SIGNOR-249513
P46531
O75056
0
binding
up-regulates activity
0.379
Furthermore, we show that Syndecan-3 interacts with Notch and is required for Notch processing by ADAM17/tumor necrosis factor-€“converting enzyme (TACE) and signal transduction. Together, our data support the conclusion that Syndecan-3 and Notch cooperate in regulating homeostasis of the satellite cell population and ...
SIGNOR-244072
Q92918
P00519
0
phosphorylation
up-regulates activity
0.379
C-Abl phosphorylates HPK1 in cytoplasm and stimulates HPK1 activity. the c-Abl phosphorylation site (YXXP) in HPK1 (Y232QPP; aa 232–235) is localized in HPK1-KD
SIGNOR-251429
P78352
Q9UIJ5
0
palmitoylation
up-regulates activity
0.379
Plasma membrane targeting of DHHC2 palmitoyltransferase rapidly recruited PSD-95 to the plasma membrane and proved essential for postsynaptic nanodomain formation.
SIGNOR-261290
O60563
P53350
0
phosphorylation
down-regulates activity
0.379
 Further analysis indicated that Plk1 could phosphorylate cyclin T1 at Ser564 and inhibit the kinase activity of cyclin T1/Cdk9 complex on phosphorylation of the C-terminal domain (CTD) of RNA polymerase II. 
SIGNOR-276501
P31749
Q5S007
0
phosphorylation
up-regulates
0.379
Expression of wild-type LRRK2 promoted neuronal survival against apoptosis through activation of the downstream effector, Akt by phosphorylation of Ser473. Phosphorylated Akt in turn inhibited FOXO 1 signaling
SIGNOR-252598
P55010
P67870
0
phosphorylation
up-regulates activity
0.379
Mass spectrometric analysis of maximally in vitro phosphorylated eIF5 localized the major phosphorylation sites at Ser-387 and Ser-388 near the C-terminus of eIF5. These serine residues are embedded within a cluster of acidic amino acid residues and account for nearly 90% of the total in vitro eIF5 phosphorylation. A m...
SIGNOR-251070
Q92793
Q07666
0
binding
down-regulates activity
0.379
These results suggest that Sam68 and CBP interact in vivo in a manner dependent on the FXE/DXXXL motif. Sam68 Represses CBP-dependent Transcriptional Activation
SIGNOR-266202
Q92813
Q9Y385
0
ubiquitination
down-regulates quantity by destabilization
0.379
ER residency places D2 physically close to an array of proteins that interact and modify the D2 molecule via ubiquitination and targeting to the proteasomal system, explaining its relatively short half-life. Both ubiquitin conjugases UBC6 and or UBC7 interact with D2 and support D2 ubiquitination. Two Lys residues in D...
SIGNOR-267481
P14866
Q16566
0
phosphorylation
up-regulates
0.379
Here we show that the regulation of the stress axis-regulated exon of the slo1 potassium channel transcripts by membrane depolarization requires a highly conserved camkiv target serine (ser-513) of the heterogeneous ribonucleoprotein l. Ser-513 phosphorylation within the rna recognition motif 4 enhanced heterogeneous r...
SIGNOR-197367
Q92569
Q9UM73
0
phosphorylation
up-regulates activity
0.379
Subsequent studies revealed that ALK promoted cell migration through the P3K-AKT pathway via the p55γ regulatory subunit of PI3K.
SIGNOR-253217
P10644
O43164
0
polyubiquitination
down-regulates quantity by destabilization
0.379
Praja2 controls the stability of PKA regulatory subunits. Praja2 ubiquitylates RIIα/β subunits. Subunits
SIGNOR-271855
P17861-2
Q96EB6
0
deacetylation
down-regulates activity
0.379
P300 increases the acetylation and protein stability of XBP1s, and enhances its transcriptional activity, whereas SIRT1 deacetylates XBP1s and inhibits its transcriptional activity.. The mRNA encoding the active spliced form of XBP1 (XBP1s) is generated from the unspliced form by IRE1 (inositol-requiring enzyme 1) duri...
SIGNOR-260430
Q96RD7
P12931
0
phosphorylation
up-regulates activity
0.379
We recently identified amino acids 198-200 (YLK) on the PANX1 intracellular loop that are critical for α1-AR-mediated vasoconstriction and PANX1 channel function. We report herein that the YLK motif is contained within an SRC homology 2 domain and is directly phosphorylated by SRC proto-oncogene, nonreceptor tyrosine k...
SIGNOR-277817
P49715
P49841
0
phosphorylation
up-regulates activity
0.379
Glycogen synthase kinase 3 (GSK3) phosphorylates T222 and T226, causing a conformational change in C/EBPα. GSK3-mediated phosphorylation does not, in itself, dramatically alter the activity of C/EBPα in our assays. phosphorylation of C/EBPalpha and other substrates by GSK3 may be required for adipogenesis, since treatm...
SIGNOR-251231
P24385
O15111
0
phosphorylation
down-regulates
0.379
Ikkalpha regulates subcellular localization and proteolysis of cyclin d1 by phosphorylation of cyclin d1 at thr286.
SIGNOR-139570
P41970
Q16539
0
phosphorylation
up-regulates
0.379
Tcf sap-1a is efficiently phosphorylated by p38 map kinase in vitro and in vivo on the homologous residues ser381 and ser387. Mutation of these sites to alanine severely reduces c-fos sre-dependent transcription mediated by sap-1a and p38 map kinase.
SIGNOR-47685
P07202
P43699
0
transcriptional regulation
up-regulates quantity by expression
0.379
TSH regulates TPO expression through the cAMP pathway and acts with thyroid-specific transcription factors such as TTF-1, TTF-2 and Pax-8.
SIGNOR-267278
P31749
Q8N5S9
0
phosphorylation
up-regulates activity
0.379
Protein kinase B (PKB) was recently reported to be activated on the phosphorylation of Thr(308) by Ca(2+)/calmodulin-dependent protein kinase kinase alpha (CaM-kinase kinase alpha), suggesting that PKB was regulated through not only the phosphoinositide 3-kinase pathway but also the Ca(2+)/calmodulin protein kinase pat...
SIGNOR-252609
O15379
P60510
0
dephosphorylation
down-regulates activity
0.379
Here we demonstrate that, in addition to protein-protein interactions with NCoR/SMRT, the activity of HDAC3 is regulated by both phosphorylation and dephosphorylation. A protein kinase CK2 phosphoacceptor site in the HDAC3 protein was identified at position Ser424, which is a nonconserved residue among the class I HDAC...
SIGNOR-248548
Q9Y6K1
P19544
0
transcriptional regulation
up-regulates quantity by expression
0.379
Here, we show that Wilms' tumour 1 (WT1), a developmental master regulator that can also act as a tumour suppressor or oncoprotein, transcriptionally regulates the de novo DNA methyltransferase 3A (DNMT3A) and that cellular WT1 levels can influence DNA methylation of gene promoters genome-wide. we demonstrate that depl...
SIGNOR-255904
Q86WG3
Q9UNE7
0
polyubiquitination
down-regulates quantity by destabilization
0.378
CHIP e.g. was found to efficiently polyubiquitinate caytaxin in vitro, suggesting that it might influence caytaxin degradation in vivo.
SIGNOR-272651
P35222
P23471
0
dephosphorylation
up-regulates activity
0.378
PTPRZ1 constitutively promotes the tyrosine dephosphorylation of \u03b2-catenin, and thus \u03b2-catenin participation in TCF-mediated transcription.
SIGNOR-277044
P06213
Q05209
0
dephosphorylation
down-regulates
0.378
Interestingly, all PTPs that were tested could completely dephosphorylate the receptor, given sufficient time, including a negative control (PTP-PEST) that failed to bind IRK as a trapping mutant.
SIGNOR-75894
P02452
P03956
0
cleavage
down-regulates quantity by destabilization
0.378
In vitro, MMP1 initiates degradation of native fibrillar collagens, crucial components of vertebrate extracellular matrix (ECM), by cleaving the peptide bond between Gly775–Ile776 or Gly775–Lys776 in native type I, II or III collagen molecules3,4. 
SIGNOR-272336
P30260
P68400
0
phosphorylation
up-regulates
0.378
We report here that phosphorylation of cdc27, a core subunit of apc, in response to tgf- signaling can facilitate the activation of apc.we have demonstrated that casein kinase ii (ckii) is involved in the phosphorylation of cdc27 in response to tgf- signaling.
SIGNOR-170872
Q96KG7
P12931
0
phosphorylation
up-regulates activity
0.378
Our data suggest that c-Src augments the phosphorylation of MEGF10 and helps form a signaling complex.|These results indicate that overexpressed MEGF10 is phosphorylated by c-Src.
SIGNOR-279290
P06493
P27707
0
binding
down-regulates activity
0.378
We demonstrate that dCK interacts with cyclin-dependent kinase 1 (Cdk1) after IR and that the interaction inhibits Cdk1 activity both in vitro and in vivo.
SIGNOR-275805
Q13362-1
Q13315
0
phosphorylation
up-regulates activity
0.378
In the present study, we demonstrate that ataxia-telangiectasia mutated (ATM) directly phosphorylates and specifically regulates B56γ3, B56γ2 and B56δ, after DNA damage. We further show that phosphorylation of B56γ3 at Ser510 leads to an increase in B56γ3-PP2A complexes, and direction of PP2A phosphatase activity towar...
SIGNOR-276318
Q15796
Q9Y5K5
0
deubiquitination
up-regulates
0.378
Here, we report a novel interaction between smads and ubiquitin c-terminal hydrolase uch37, a deubiquitinating enzyme that could potentially reverse smurf-mediated ubiquitination. In gst pull down experiments, uch37 bound weakly to smad2 and smad3, and bound very strongly to smad7 in a region that is distinct from the ...
SIGNOR-138876
P09471
P28566
0
binding
up-regulates activity
0.378
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-256984
Q8IWV1
P43405
0
phosphorylation
up-regulates activity
0.378
Upon stimulation via the B or T cell receptors, LAX is rapidly phosphorylated by Src and Syk family tyrosine kinases and interacts with Grb2, Gads, and p85. 
SIGNOR-273535
P49841
Q15831
0
phosphorylation
down-regulates activity
0.378
In this regard, it was shown that LKB1 physically associates with PKC-zeta, which is known to inhibit GSK3beta kinase activity by promoting its phosphorylation.|Thus, inhibitory phosphorylation of GSK3beta by LKB1 and/or AKT may be a cardinal event leading to constitutive activation of Wnt and beta-catenin signaling (X...
SIGNOR-280148
P46531
O75553
0
binding
up-regulates
0.378
The induction of disabled-1 (dab-1) tyrosine phosphorylation, and the subsequent activation of src family kinases, were found to be essential steps for the activation of notch-1 signaling by reelin
SIGNOR-196438
Q9NQB0
P19784
0
phosphorylation
up-regulates activity
0.378
We show here that Tcf-4 can be phosphorylated in vitro by protein kinase CK2 stoichiometrically in amino acids Ser-58-Ser-59-Ser-60. Phosphorylation of these residues does not modify the interaction of Tcf-4 with beta-catenin but reduces its association to plakoglobin. | Experiments performed using a Tcf-4 mutant with ...
SIGNOR-251044
P35240
P62140
0
dephosphorylation
up-regulates
0.378
When serine 518 is dephosphorylated by the myosin phosphatase mypt-1-pp1?, The tumor suppressor function of merlin is activated, inhibiting the ras signaling pathway and leading to growth arrest
SIGNOR-159836
P31749
O75925
0
sumoylation
up-regulates activity
0.378
Although multiple sites on Akt could be SUMOylated, K276 was identified as a major SUMO acceptor site. K276R or E278A mutation reduced SUMOylation of Akt but had little effect on its ubiquitination. Strikingly, these mutations also completely abolished Akt kinase activity. In support of these results, we found that exp...
SIGNOR-252735
Q9Y6Q9
O14920
0
phosphorylation
up-regulates activity
0.378
We demonstrated the in vitro phosphorylation of SRC-3 by the two catalytic subunits of the IKK complex, IKKα and IKKβ.  IKK kinase activity is required for synergistic activation with SRC-3
SIGNOR-251298
P42768
P07948
0
phosphorylation
up-regulates activity
0.378
We demonstrated that WASP is phosphorylated on tyrosine 291 in macrophages, and the WASP phosphorylation is important for the phagocytic cup formation. In addition, we showed that WASP and WASP-interacting protein (WIP) form a complex at the phagocytic cup and that the WASP.WIP complex plays a critical role in the phag...
SIGNOR-273959
P10244
Q13309
0
polyubiquitination
down-regulates quantity by destabilization
0.378
P19Skp1 and Cul-1 bind to the F-box protein p45Skp2 to form a complex (SCF) that functions as E3 ubiquitin ligase.We show that B-Myb physically and functionally interacts with components of the Cdc34-SCFp45Skp2 ubiquitin pathway and propose that B-Myb degradation may be required for controlling the correct alternation ...
SIGNOR-272572
P63167
Q13153
0
phosphorylation
down-regulates
0.378
Dlc1 phosphorylation on ser(88) by p21-activated kinase 1 (pak1), a signaling nodule, promotes mammalian cell survival by regulating its interaction with bim and the stability of bim. Here we discovered that phosphorylation of ser(88), which juxtapose each other at the interface of the dlc dimer, disrupts dlc1 dimer fo...
SIGNOR-159995
Q13362-3
Q13315
0
phosphorylation
up-regulates activity
0.378
In the present study, we demonstrate that ataxia-telangiectasia mutated (ATM) directly phosphorylates and specifically regulates B56γ3, B56γ2 and B56δ, after DNA damage. We further show that phosphorylation of B56γ3 at Ser510 leads to an increase in B56γ3-PP2A complexes, and direction of PP2A phosphatase activity towar...
SIGNOR-276320
P04629
P18031
0
dephosphorylation
down-regulates activity
0.378
PTP1B inactivation prevents TrkA exit from soma and causes receptor degradation, suggesting a " gate-keeper " mechanism that ensures targeting of inactive receptors to axons to engage with ligand.|We identify a gate keeping mechanism in which TrkA receptors, destined for transcytosis, are dephosphorylated in neuronal s...
SIGNOR-277081