Prophage ACR Screening Pipelines
This directory contains two analysis pipelines for screening Anti-CRISPR (Acr) proteins from prophage genomes.
Directory Structure
| Folder | Description |
|---|---|
| double_diff/ | Double-divergence screening pipeline: identifies novel Acr candidates that are significantly different from known Acr proteins at both sequence and structure levels |
| double_similar/ | High-confidence Acr identification pipeline: identifies high-confidence Acr candidates that are highly similar to known Acr proteins at both sequence and structure levels |
Quick Navigation
- double_diff — For discovery of Acrs with novel mechanisms. Excludes proteins with sequence homology (qcovs≥30, pident≥30) or structural similarity to known Acrs, retaining "double-divergent" candidates.
- double_similar — For expansion of known Acr families. Applies stringent filters for sequence (qcovs≥50, pident>50) and structural (TM-score>0.7, prob=1.0) similarity to obtain high-confidence candidates.
Detailed Documentation
Each folder contains a comprehensive README document describing:
- Screening strategies and workflows
- Input and output data formats
- Software tools and parameter settings
- Screening thresholds and criteria
Click the links above to view detailed documentation for each pipeline.