id
stringlengths
15
19
title
stringlengths
4
685
content
stringlengths
54
6.04k
contents
stringlengths
77
6.1k
PMID
int64
21
3.77M
pubmed23n0001_300
[Effect of peripheral counterpulsation on the body of an animal with intact heart].
The effect of lasting peripheral counterpulsation upon the haemodynamics and the main biochemical factors of the blood was studied in 14 dogs with intact hearts. In cases of significant tachycardia counterpulsation in a 1:2 regimen results in only a partial reduction of the resistance to the cardiac output. This does n...
[Effect of peripheral counterpulsation on the body of an animal with intact heart]. The effect of lasting peripheral counterpulsation upon the haemodynamics and the main biochemical factors of the blood was studied in 14 dogs with intact hearts. In cases of significant tachycardia counterpulsation in a 1:2 regimen resu...
534
pubmed23n0001_301
[Regulation of interrelation between pulmonary ventilation and circulation].
In acute experiments on 92 cats anesthetized with Urethane and kept under controlled respiration the mechanism of tonic activity of the pulmonary vessels was studied in the presence of a decreased partial pressure of oxygen in the alveoli. The tonicity of the pulmonary vessels was recorded during autoperfusion of the v...
[Regulation of interrelation between pulmonary ventilation and circulation]. In acute experiments on 92 cats anesthetized with Urethane and kept under controlled respiration the mechanism of tonic activity of the pulmonary vessels was studied in the presence of a decreased partial pressure of oxygen in the alveoli. The...
535
pubmed23n0001_302
Multiple cyclic nucleotide phosphodiesterases in rat kidney.
Using DEAE-cellulose chromatography and Agarose gel filtration we have partially purified a low Km cyclic adenosine monophosphate (AMP) phosphodiesterase from the 100,000 X g supernatant of rat kidneys. The characteristics of this enzyme included a Km of approximately 4 muM a pH optimum of around 8.0 and a requirement ...
Multiple cyclic nucleotide phosphodiesterases in rat kidney. Using DEAE-cellulose chromatography and Agarose gel filtration we have partially purified a low Km cyclic adenosine monophosphate (AMP) phosphodiesterase from the 100,000 X g supernatant of rat kidneys. The characteristics of this enzyme included a Km of appr...
537
pubmed23n0001_303
Effect on aging on plasma renin and aldosterone in normal man.
The influence of aging on the renin-angiotensin-aldosterone system was evaluated by comparing young (20 to 30 yr) with elderly (62 to 70 yr) healthy subjects. Despite comparable body sodium-fluid balance in the two age groups, serum renin concentration, plasma renin activity and aldosterone concentrations were lower in...
Effect on aging on plasma renin and aldosterone in normal man. The influence of aging on the renin-angiotensin-aldosterone system was evaluated by comparing young (20 to 30 yr) with elderly (62 to 70 yr) healthy subjects. Despite comparable body sodium-fluid balance in the two age groups, serum renin concentration, pla...
538
pubmed23n0001_304
[Immune mechanisms in uremia].
There is both clinical and experimental evidence that cellular and humoral immunity are suppressed in patients with renal insufficiency: observations in organ transplantation and in vitro stimulation of lymphocytes from uraemic patients, investigations of acute and late hypersensitivity reactions, the immune response a...
[Immune mechanisms in uremia]. There is both clinical and experimental evidence that cellular and humoral immunity are suppressed in patients with renal insufficiency: observations in organ transplantation and in vitro stimulation of lymphocytes from uraemic patients, investigations of acute and late hypersensitivity r...
540
pubmed23n0001_305
Improvement of renin determination in human plasma using a commonly available renin standard in a radioimmunological method.
A new method for the measurement of renin in human plasma is described. The method is based on the introduction of the internationally available renin standard of the Medical Research Council (MRC) London, as a calibration system. Thus, some principal disadvantages of methods expressing results in renin reaction veloci...
Improvement of renin determination in human plasma using a commonly available renin standard in a radioimmunological method. A new method for the measurement of renin in human plasma is described. The method is based on the introduction of the internationally available renin standard of the Medical Research Council (MR...
541
pubmed23n0001_306
[Validity of pH measurements by means of micro pH combination electrodes in blood and other biological fluids (author's transl)].
pH measurements in blood or in media containing either proteins or polypeptides and performed by means of micro pH combination electrodes type N 58 (Schott & Gen., Mainz) yield in a systematic error according to the regression line y = 1.135 chi - 0.842, in the range between pH 5.3 and 8.3. This deviation from the ...
[Validity of pH measurements by means of micro pH combination electrodes in blood and other biological fluids (author's transl)]. pH measurements in blood or in media containing either proteins or polypeptides and performed by means of micro pH combination electrodes type N 58 (Schott & Gen., Mainz) yield in a syst...
542
pubmed23n0001_307
The effects of drinking on offspring: an historical survey of the American and British literature.
Current research on the effects on offspring of drinking during pregnancy has revived interest in an extremely old topic. Observations made during England's Gin Epidemic (1720-1750) were followed by warnings of 19th-century medical writers that parental drinking could damage the fetus. Many concurring studies were repo...
The effects of drinking on offspring: an historical survey of the American and British literature. Current research on the effects on offspring of drinking during pregnancy has revived interest in an extremely old topic. Observations made during England's Gin Epidemic (1720-1750) were followed by warnings of 19th-centu...
545
pubmed23n0001_308
[Glutathione (author's transl)].
Glutathione plays an important role in biology and medicine. Most cells of plants and animals contain high concentrations of reduced glutathione and a much smaller amount of oxidised glutathione. GSH is important for several metabolic functions of live cells, e.g. the protection of oxidative stress by peroxides, mediat...
[Glutathione (author's transl)]. Glutathione plays an important role in biology and medicine. Most cells of plants and animals contain high concentrations of reduced glutathione and a much smaller amount of oxidised glutathione. GSH is important for several metabolic functions of live cells, e.g. the protection of oxid...
543
pubmed23n0001_309
Hyperexcitability in the neural substrate of emotional behavior in cats after alcohol withdrawal. Evidence of a rapid development of alcohol dependence.
Substantial and prolonged withdrawal hyperexcitability in the neural substrate for affective defense was revealed by behavioral and electrophysiological measures in cats exposed to moderate to heavy doses of alcohol for periods ranging from 6 to 72 hours. The data are interpreted as indicating a rapid development of ph...
Hyperexcitability in the neural substrate of emotional behavior in cats after alcohol withdrawal. Evidence of a rapid development of alcohol dependence. Substantial and prolonged withdrawal hyperexcitability in the neural substrate for affective defense was revealed by behavioral and electrophysiological measures in ca...
549
pubmed23n0001_310
Interactions of age, sex and long-term alcohol intake in selectively bred strains of rats.
The alcohol consumption by five genotypes of rats was studied in two experiments. Alcohol intake was age-dependent in rats bred for high emotional reactivity and avoidance conditionability. Differences in consumption by sex appeared to be primarily due to differences in body weight.
Interactions of age, sex and long-term alcohol intake in selectively bred strains of rats. The alcohol consumption by five genotypes of rats was studied in two experiments. Alcohol intake was age-dependent in rats bred for high emotional reactivity and avoidance conditionability. Differences in consumption by sex appea...
550
pubmed23n0001_311
Regional study of acid hydrolases and lysosomal membrane properties in the normal human brain at various ages.
Acid hydrolases and lysosomal membrane properties were studied at various ages in the normal human brain. In CSF and four brain regions, the inferior olive, the cerebellar cortex, the caudate nucleus and the frontal cortex were thus beta-galactosidase, beta-glucosidase, alpha-mannosidase, hexosaminidase and acid phosph...
Regional study of acid hydrolases and lysosomal membrane properties in the normal human brain at various ages. Acid hydrolases and lysosomal membrane properties were studied at various ages in the normal human brain. In CSF and four brain regions, the inferior olive, the cerebellar cortex, the caudate nucleus and the f...
561
pubmed23n0001_312
The late effects of selected immunosuppressants on immunocompetence, disease incidence, and mean life-span. II. Cell-mediated immune activity.
The late effects of various immunosuppressive insults on cell-mediated immunity in mice were studied in an attempt to assess the role of immune surveillance in the aging process. Results were obtained using susceptibility to allogeneic tumor cell challenge, graft-versus-host reaction (GVHR), blastogenic response to PHA...
The late effects of selected immunosuppressants on immunocompetence, disease incidence, and mean life-span. II. Cell-mediated immune activity. The late effects of various immunosuppressive insults on cell-mediated immunity in mice were studied in an attempt to assess the role of immune surveillance in the aging process...
562
pubmed23n0001_313
Lipoxygenase isozymes of peanut.
Lipoxygenase was isolated and partially purified from peanut seed by ammonium sulfate precipitation, gel filtration, and ion exchange column chromatography. Three isozymes of lipoxygenase were identified. Two had pH optima of 6.2, and the other an optimum of 8.3. Molecular weight of each isozyme was 7.3 x 10(4), as det...
Lipoxygenase isozymes of peanut. Lipoxygenase was isolated and partially purified from peanut seed by ammonium sulfate precipitation, gel filtration, and ion exchange column chromatography. Three isozymes of lipoxygenase were identified. Two had pH optima of 6.2, and the other an optimum of 8.3. Molecular weight of eac...
580
pubmed23n0001_314
Crassin acetate, the principal antineoplastic agent in four gorgonians of the Pseudoplexaura genus.
Crassin acetate, a lactonic cembrane diterpene, has been shown to be the principal antineoplastic agent present in the marine invertebrates (gorgonians) Pseudoplexaura porosa, P. flagellosa, P. wagenaari and P. crucis.
Crassin acetate, the principal antineoplastic agent in four gorgonians of the Pseudoplexaura genus. Crassin acetate, a lactonic cembrane diterpene, has been shown to be the principal antineoplastic agent present in the marine invertebrates (gorgonians) Pseudoplexaura porosa, P. flagellosa, P. wagenaari and P. crucis.
578
pubmed23n0001_315
[Computer experience and further developments in the respiratory function laboratory (author's transl)].
Reported is on satisfactory results obtained with a small-size computer consisting of punching and scanning device, as well as plain writing machine in the respiratory function laboratory. Developed in on- as well as off-line processing by an own technical staff, a diagnostic and teaching program was established for al...
[Computer experience and further developments in the respiratory function laboratory (author's transl)]. Reported is on satisfactory results obtained with a small-size computer consisting of punching and scanning device, as well as plain writing machine in the respiratory function laboratory. Developed in on- as well a...
594
pubmed23n0001_316
Phosphate transport in rat liver mitochondria. Kinetics, inhibitor sensitivity, energy requirements, and labeled components.
Experiments were carried out to define the kinetic parameters of the major phosphate transport processes of rat liver mitochondria, and to obtain information about the molecular properties of these systems.
Phosphate transport in rat liver mitochondria. Kinetics, inhibitor sensitivity, energy requirements, and labeled components. Experiments were carried out to define the kinetic parameters of the major phosphate transport processes of rat liver mitochondria, and to obtain information about the molecular properties of the...
609
pubmed23n0001_317
L-tyrosine: 2-oxoglutarate aminotransferase induction by hydrocortisone in the thymus of the white rat.
Hydrocortisone hemisuccinate within 4 hours after in vivo administration produced an increase in precursor incoporation into rat thymus RNA and proteins in the whole animal. From these results, together with information obtained from measurements of the tyrosine aminotransferase activity and the action of mitomycin C a...
L-tyrosine: 2-oxoglutarate aminotransferase induction by hydrocortisone in the thymus of the white rat. Hydrocortisone hemisuccinate within 4 hours after in vivo administration produced an increase in precursor incoporation into rat thymus RNA and proteins in the whole animal. From these results, together with informat...
610
pubmed23n0001_318
Identification of the 30 S protein adjacent to peptidyl transferase catalytic center of Escherichia coli ribosomes.
Iodoacetylphenylalanyl-tRNAPhe was used as an affinity label to localize the ribosomal components involved in the peptidyl transferase catalytic center of Escherichia coli ribosomes. When labeling was carried out at pH 5.0, the affinity label could specifically label the ribosomal components which comprise the catalyti...
Identification of the 30 S protein adjacent to peptidyl transferase catalytic center of Escherichia coli ribosomes. Iodoacetylphenylalanyl-tRNAPhe was used as an affinity label to localize the ribosomal components involved in the peptidyl transferase catalytic center of Escherichia coli ribosomes. When labeling was car...
611
pubmed23n0001_319
The value of a histamine H2-receptor antagonist in the management of patients with the Zollinger-Ellison syndrome.
Inhibition of acid secretion by an H2-receptor antagonist (metiamide) was assessed in three patients with the Zollinger-Ellison syndrome. Metiamide (200 or 300 mg) inhibited acid secretion transiently (2 1/2 hours) by 85 to 100 per cent in all patients. Although anticholinergic drugs alone inhibited acid secretion by o...
The value of a histamine H2-receptor antagonist in the management of patients with the Zollinger-Ellison syndrome. Inhibition of acid secretion by an H2-receptor antagonist (metiamide) was assessed in three patients with the Zollinger-Ellison syndrome. Metiamide (200 or 300 mg) inhibited acid secretion transiently (2 1...
616
pubmed23n0001_320
Histamine receptors in the vasculature of the rabbit ear.
Histamine has a dual action on the isolated perfused ear preparation of the rabbit. The amine induced a dose-dependent rise in perfusion pressure when the preparation was perfused with Krebs' solution. This pressor response was reversed to a depressor effect when meypramine was added to the perfusion fluid. This depres...
Histamine receptors in the vasculature of the rabbit ear. Histamine has a dual action on the isolated perfused ear preparation of the rabbit. The amine induced a dose-dependent rise in perfusion pressure when the preparation was perfused with Krebs' solution. This pressor response was reversed to a depressor effect whe...
626
pubmed23n0001_321
Relative pre- and postsynaptic potencies of alpha-adrenoceptor agonists in the rabbit pulmonary artery.
The rabbit pulmonary artery contains postsynaptic alpha-adrenoceptors which meidate smooth muscle contraction; its noradrenergic nerves contain presynaptic alpha-adrenoceptors which mediate inhibition of the release of the transmitter evoked by nerve impulses. Dose-response curves for the pre- and postsynaptic effects ...
Relative pre- and postsynaptic potencies of alpha-adrenoceptor agonists in the rabbit pulmonary artery. The rabbit pulmonary artery contains postsynaptic alpha-adrenoceptors which meidate smooth muscle contraction; its noradrenergic nerves contain presynaptic alpha-adrenoceptors which mediate inhibition of the release ...
627
pubmed23n0001_322
Investigation into some imidazoline compounds, with respect to peripheral alpha-adrenoceptor stimulation and depression of cardiovascular centers.
Peripheral alpha-adrenoceptor stimulation was tested by means of hypertensive effects of the drugs following i.v. injection in spinal rats. Naphazoline (NP), oxymetazoline (OM), St 91-2-(2,6-diethylphenylimino)-2-imidazolidine--and St 1697--2-(2-ethyl, 6-methylphenylimino)-2-imidazolidine--were 3 to 5 times more potent...
Investigation into some imidazoline compounds, with respect to peripheral alpha-adrenoceptor stimulation and depression of cardiovascular centers. Peripheral alpha-adrenoceptor stimulation was tested by means of hypertensive effects of the drugs following i.v. injection in spinal rats. Naphazoline (NP), oxymetazoline (...
628
pubmed23n0001_323
The regulation of striatal tyrosine hydroxylase. Effects of gamma hydroxybutric acid and healperidol.
Gamma-hydroxybutyric acid (GHBA) in doses that increased the striatal dopamine (DA) content of rat brain failed to increase the affinity of striatal tyrosine hydroxylase (TH) for its pterdine cofactor or to change the sensitivity of the enzyme to the inhibition by DA. Haloperidol (1 mg/kg) decreased the apparent Km of ...
The regulation of striatal tyrosine hydroxylase. Effects of gamma hydroxybutric acid and healperidol. Gamma-hydroxybutyric acid (GHBA) in doses that increased the striatal dopamine (DA) content of rat brain failed to increase the affinity of striatal tyrosine hydroxylase (TH) for its pterdine cofactor or to change the ...
629
pubmed23n0001_324
Host-tumor relationship XXXIII. Inhibitor of hyaluronidase in blood serum of cancer patients.
Inhibiting activity of blood serum was determined from the decrease of N-acetyl-hexosamine end groups of hyaluronic acid products released by testicular hyaluronidase. Maximum inhibition was observed within the region of pH from 6.5 to 6.8. Correlation between the serum concentration and its inhibiting effect on hyalur...
Host-tumor relationship XXXIII. Inhibitor of hyaluronidase in blood serum of cancer patients. Inhibiting activity of blood serum was determined from the decrease of N-acetyl-hexosamine end groups of hyaluronic acid products released by testicular hyaluronidase. Maximum inhibition was observed within the region of pH fr...
630
pubmed23n0001_325
Influence of peritoneal dialysis on factors affecting oxygen transport.
To determine the effect of changing concentrations of uremic metabolites on factors affecting oxygen transport, without the effects of extracorporeal blood pumping, we studied five patients before, during and after peritoneal dialysis. Significant decreases in serum urea, creatinine and phosphate and increase in serum ...
Influence of peritoneal dialysis on factors affecting oxygen transport. To determine the effect of changing concentrations of uremic metabolites on factors affecting oxygen transport, without the effects of extracorporeal blood pumping, we studied five patients before, during and after peritoneal dialysis. Significant ...
631
pubmed23n0001_326
Some effects of ammonium salts on renal histology and function in the dog.
NH4Cl was infused into the left renal artery of anesthetized dogs at 50-125 mum/kg/min for up to 110 min. Renal blood flow declined early then increased to supra-control levels during infusion. Kidneys perfused at 125 mum/kg/min for 90 min showed patchy to confluent mixtures of cortical necrosis and tubular necrosis. E...
Some effects of ammonium salts on renal histology and function in the dog. NH4Cl was infused into the left renal artery of anesthetized dogs at 50-125 mum/kg/min for up to 110 min. Renal blood flow declined early then increased to supra-control levels during infusion. Kidneys perfused at 125 mum/kg/min for 90 min showe...
632
pubmed23n0001_327
Papillary necrosis associated with calyceal arteritis.
The renal papilla has a double blood supply - from both the vasa recta and the calyceal arteries. The importance of the latter supply is not established. A case of polyarteritis associated with papillary necrosis is reported, in which the calyceal vessels, supplying the area, show acute necrotizing arteritis and occlus...
Papillary necrosis associated with calyceal arteritis. The renal papilla has a double blood supply - from both the vasa recta and the calyceal arteries. The importance of the latter supply is not established. A case of polyarteritis associated with papillary necrosis is reported, in which the calyceal vessels, supplyin...
633
pubmed23n0001_328
Fasting uric acid and phosphate in urine and plasma of renal calcium-stone formers.
Clearance experiments in calcium-stone patients (n = 60) and controls (n = 60) demonstrated significantly higher urinary uric acid (UA) in younger (less 40 years) stone patients than controls (median: 480 vs. 351 mug/min) but not in older (greater than 40 years) patients. Serum UA and urinary oxypurines were comparable...
Fasting uric acid and phosphate in urine and plasma of renal calcium-stone formers. Clearance experiments in calcium-stone patients (n = 60) and controls (n = 60) demonstrated significantly higher urinary uric acid (UA) in younger (less 40 years) stone patients than controls (median: 480 vs. 351 mug/min) but not in old...
634
pubmed23n0001_329
Effects of varying dialysate calcium concentrations on the plasma calcium fractions in patients on dialysis.
The plasma-ionized calcium levels decreased during haemodialysis when a dialysate calcium concentration of 5 mg/100 ml was used. When dialysis was performed with a bath calcium concentration of 7.5 mg/100 ml, there was a significant increase in the plasm-ionized calcium levels in the post-dialysis period. These results...
Effects of varying dialysate calcium concentrations on the plasma calcium fractions in patients on dialysis. The plasma-ionized calcium levels decreased during haemodialysis when a dialysate calcium concentration of 5 mg/100 ml was used. When dialysis was performed with a bath calcium concentration of 7.5 mg/100 ml, th...
635
pubmed23n0001_330
[Borderline between antiepileptic and psychopharmacological drugs (author's transl)].
From the chemical and pharmalogical point of view it is impossible to separate strictly between antiepileptic and psychopharmalogical drugs. Inspite of that and several clinical overlappings it is possible and helpful finding differences between the two groups of drugs. Basing on the indication we try to demonstrate th...
[Borderline between antiepileptic and psychopharmacological drugs (author's transl)]. From the chemical and pharmalogical point of view it is impossible to separate strictly between antiepileptic and psychopharmalogical drugs. Inspite of that and several clinical overlappings it is possible and helpful finding differen...
643
pubmed23n0001_331
Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei.
Soluble glycoproteins have been purified from a series of clones of Trypanosoma brucei 427. Each clone yielded a characteristic predominant glycoprotein which induced clone-specific immunity to trypanosome infection in mice. These glycoproteins were shown by specific labelling and enzyme digestion of cells to be the ma...
Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Soluble glycoproteins have been purified from a series of clones of Trypanosoma brucei 427. Each clone yielded a characteristic predominant glycoprotein which induced clone-specific i...
645
pubmed23n0001_332
The determination of gamma-glutamyl transpeptidase by reaction rate assay at 37 degrees C.
The determination of gamma-glutamyl transpeptidase by a reaction rate assay using optimal reaction conditions at 37 degrees is described. Specific conditions and instrument settings are described for the LKB Reaction Rate Analyser but the actual assay conditions are applicable to any similar reaction rate system. The p...
The determination of gamma-glutamyl transpeptidase by reaction rate assay at 37 degrees C. The determination of gamma-glutamyl transpeptidase by a reaction rate assay using optimal reaction conditions at 37 degrees is described. Specific conditions and instrument settings are described for the LKB Reaction Rate Analyse...
646
pubmed23n0001_333
The influence of cortisol on the enzymes of fatty acid synthesis in developing mammalian lung and brain.
Administration of cortisol to fetal rabbits resulted in a 42% inhibition of pulmonary de novo fatty acid synthesis from acetyl coenzyme A (CoA) (P = less than 0.025). This was associated with inhibition of acetyl-CoA carboxylase (EC. 6.4.1.2.) activity (P = less than 0.01) and a tendency towards decreased activity of f...
The influence of cortisol on the enzymes of fatty acid synthesis in developing mammalian lung and brain. Administration of cortisol to fetal rabbits resulted in a 42% inhibition of pulmonary de novo fatty acid synthesis from acetyl coenzyme A (CoA) (P = less than 0.025). This was associated with inhibition of acetyl-Co...
648
pubmed23n0001_334
The relation between myosin adenosinetriphosphatase activity and inactivation of myosin under alkaline conditions of heart muscles in mammals of different size.
ATPase activity of myosin in the heart muscle of the mouse, rat, guinea-pig, rabbit and pig was studied at neutral pH and under mild alkaline conditions. At neutral pH the ATPase activity of myosin is inversely related to body size of the animal species. The decrease of ATPase activity of myosin after alkaline preincub...
The relation between myosin adenosinetriphosphatase activity and inactivation of myosin under alkaline conditions of heart muscles in mammals of different size. ATPase activity of myosin in the heart muscle of the mouse, rat, guinea-pig, rabbit and pig was studied at neutral pH and under mild alkaline conditions. At ne...
651
pubmed23n0001_335
Carbon dioxide response curves during hypothermia.
The responsiveness of the medullary chemoreceptors, measured by the ventilatory response to hypercapnia given in an hyperoxic gas mixture in intact anesthetized dogs has been evaluated during normothermia and at two levels of hypothermia. The response was studied in: 1) 20 dogs during normothermia, 2) 10 of these dogs ...
Carbon dioxide response curves during hypothermia. The responsiveness of the medullary chemoreceptors, measured by the ventilatory response to hypercapnia given in an hyperoxic gas mixture in intact anesthetized dogs has been evaluated during normothermia and at two levels of hypothermia. The response was studied in: 1...
652
pubmed23n0001_336
[Zollinger-Ellison syndrome treated medically by an inhibitor of H2 histamine receptors].
Metiamide an histamine H2-receptors antagonist has been used to treat a case of Zollinger-Ellison syndrome characterized by a long standing diarrhea, an important gastric hypersecretion and a moderatly elevated plasma gastrin but without digestive ulceration. At the dose of 600 mg per day, Metiamide induced a complete ...
[Zollinger-Ellison syndrome treated medically by an inhibitor of H2 histamine receptors]. Metiamide an histamine H2-receptors antagonist has been used to treat a case of Zollinger-Ellison syndrome characterized by a long standing diarrhea, an important gastric hypersecretion and a moderatly elevated plasma gastrin but ...
653
pubmed23n0001_337
[Exercise test in the asthmatic patient. Study of 75 patients].
Frequency of exercise-induced asthma has been studied in 75 unselected asthmatic patients (adults and children) by measuring the forced expiratory volume at one second (FEV1) and the vital capacity (VC) before and after a treadmill exercise, continued until heart rate was at least equal to 80 p.cent-85 p.cent of maximu...
[Exercise test in the asthmatic patient. Study of 75 patients]. Frequency of exercise-induced asthma has been studied in 75 unselected asthmatic patients (adults and children) by measuring the forced expiratory volume at one second (FEV1) and the vital capacity (VC) before and after a treadmill exercise, continued unti...
656
pubmed23n0001_338
[Anaphylactic accidents due to glaphenine. 5 cases].
The authors report 5 cases of shock type anaphylactic reactions after the ingestion of glafenine. Up to the present, acute tubulo-interstitial nephritis had chiefly been reported. The existence of allergy to this medication remains to be proved by the wider application of allergological studies.
[Anaphylactic accidents due to glaphenine. 5 cases]. The authors report 5 cases of shock type anaphylactic reactions after the ingestion of glafenine. Up to the present, acute tubulo-interstitial nephritis had chiefly been reported. The existence of allergy to this medication remains to be proved by the wider applicati...
657
pubmed23n0001_339
Intranasal betamethasone valerate in seasonal rhinitis.
A double-blind study comparing betamethasone valerate and placebo aerosols was carried out in 40 patients with a history of seasonal allergic rhinitis and positive skin tests to grass pollens. Analysis of the symptoms recorded on a daily record card for a period of one month indicated that the mean monthly symptom-scor...
Intranasal betamethasone valerate in seasonal rhinitis. A double-blind study comparing betamethasone valerate and placebo aerosols was carried out in 40 patients with a history of seasonal allergic rhinitis and positive skin tests to grass pollens. Analysis of the symptoms recorded on a daily record card for a period o...
665
pubmed23n0001_340
Urinary acidification in renal allografts.
Urinary acid excretion was measured in 35 human renal allograft recipients during the first few days after the onset of diuresis and two months after transplantation. In 16 out of 17 rejection episodes an early significant decrease of renal total net H+ excretion was observed. This urinary acidification impairment prec...
Urinary acidification in renal allografts. Urinary acid excretion was measured in 35 human renal allograft recipients during the first few days after the onset of diuresis and two months after transplantation. In 16 out of 17 rejection episodes an early significant decrease of renal total net H+ excretion was observed....
666
pubmed23n0001_341
A simple test for early detection of severe renal homograft rejection.
Adding urine to a standard buffered fibrinogen solution and then coagulating it with thrombin gives reproducible coagulation times with normal urines. Coagulation of fibrinogen by thrombin is prolonged in acid solutions with a pH below six. Urines of high acidity lower the buffer pH of the fibrinogen solution below a v...
A simple test for early detection of severe renal homograft rejection. Adding urine to a standard buffered fibrinogen solution and then coagulating it with thrombin gives reproducible coagulation times with normal urines. Coagulation of fibrinogen by thrombin is prolonged in acid solutions with a pH below six. Urines o...
667
pubmed23n0001_342
Catalysis by acetylcholinesterase: evidence that the rate-limiting step for acylation with certain substrates precedes general acid-base catalysis.
Inferences about the catalytic mechanism of acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) are frequently made on the basis of a presumed analogy with chymotrypsin, EC 3.4.21.1. Although both enzymes are serine hydrolases, several differences in the steady-state kinetic properties of the two have been obser...
Catalysis by acetylcholinesterase: evidence that the rate-limiting step for acylation with certain substrates precedes general acid-base catalysis. Inferences about the catalytic mechanism of acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) are frequently made on the basis of a presumed analogy with chymotryp...
668
pubmed23n0001_343
Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes.
The light-induced absorbance change at 518 nm of isolated chloroplasts consists of a rapid phase, and a slow phase which is complete in about 20 sec. The slow component of the 518 nm absorbance change correlates with the light-induced change in 90 degrees light scattering at 518 nm. Both show a similar time course, sim...
Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes. The light-induced absorbance change at 518 nm of isolated chloroplasts consists of a rapid phase, and a slow phase which is complete in about 20 sec. The slow component of the 518 nm absorbance change correlates wi...
669
pubmed23n0001_344
phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.
Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside triphosphates, and ATP, and is s...
phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli. Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. ...
670
pubmed23n0001_345
Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: a monitor of catalytic events.
The intensely chromophoric intramolecular coordination complex formed between arsanilazotyrosine-248 and the active site zinc atom of azocarboxypeptidase A (Johansen, J. T. & Vallee, B. L. (1971) Proc. Nat. Acad. Sci. USA 68, 2532-2535) is a spectrokinetic probe of catalytic events. The interconversion of the azoTy...
Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: a monitor of catalytic events. The intensely chromophoric intramolecular coordination complex formed between arsanilazotyrosine-248 and the active site zinc atom of azocarboxypeptidase A (Johansen, J. T. & Vallee, B. L. (1971) Proc. Nat. Acad. ...
671
pubmed23n0001_346
Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli.
Addition of ubiquinone-1 to E. coli ML 308-225 membrane vesicles dramatically increases coupling between NADH oxidation and active transport such that initial rates and steady-state levels of lactose and amino-acid accumulation are comparable to those observed during D-lactate oxidation. Similar but less dramatic effec...
Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli. Addition of ubiquinone-1 to E. coli ML 308-225 membrane vesicles dramatically increases coupling between NADH oxidation and active transport such that initial rates and steady-state levels of lactose and a...
672
pubmed23n0001_347
Protection of lethally irradiated mice with allogeneic fetal liver cells: influence of irradiation dose on immunologic reconstitution.
After lethal irradiation long-lived, immunologically vigorous C3Hf mice were produced by treatment with syngeneic fetal liver cells or syngeneic newborn or adult spleen cells. Treatment of lethally irradiated mice with syngeneic or allogeneic newborn thymus cells or allogeneic newborn or adult spleen cells regularly le...
Protection of lethally irradiated mice with allogeneic fetal liver cells: influence of irradiation dose on immunologic reconstitution. After lethal irradiation long-lived, immunologically vigorous C3Hf mice were produced by treatment with syngeneic fetal liver cells or syngeneic newborn or adult spleen cells. Treatment...
673
pubmed23n0001_348
Mechanism of action of penicillin: triggering of the pneumococcal autolytic enzyme by inhibitors of cell wall synthesis.
During penicillin treatment of an autolysin defective mutant pneumococcus we have observed three novel phenomena: (i) Growth of the mutant cultures is inhibited by the same concentrations of penicillin that induce lysis in the wild type. (ii) Mutant bacteria treated with the minimum growth inhibitory concentration of p...
Mechanism of action of penicillin: triggering of the pneumococcal autolytic enzyme by inhibitors of cell wall synthesis. During penicillin treatment of an autolysin defective mutant pneumococcus we have observed three novel phenomena: (i) Growth of the mutant cultures is inhibited by the same concentrations of penicill...
674
pubmed23n0001_349
Identification of NADPH-thioredoxin reductase system in Euglena gracillis.
Euglena gracilis contains a protein system which can utilize the reducing power of NADPH in the ribonucleotide reductase-catalyzed reduction of CTP. The proteins required for this reaction are a flavoprotien with a molecular weight of approximately 185,000 which is functionally similar to thioredoxin reductase (NADPH),...
Identification of NADPH-thioredoxin reductase system in Euglena gracillis. Euglena gracilis contains a protein system which can utilize the reducing power of NADPH in the ribonucleotide reductase-catalyzed reduction of CTP. The proteins required for this reaction are a flavoprotien with a molecular weight of approximat...
675
pubmed23n0001_350
Purification of folate binding factor in normal umbilical cord serum.
Human umbilical cord serum was found to contain both free folate and folate complexed to a high-molecular weight factor. The complexed folate was bound to a very high affinity binder and was present in concentrations equivalent to as much as 60 ng of 5-methyltetrahydrofolic acid per ml of serum. Acidification of the se...
Purification of folate binding factor in normal umbilical cord serum. Human umbilical cord serum was found to contain both free folate and folate complexed to a high-molecular weight factor. The complexed folate was bound to a very high affinity binder and was present in concentrations equivalent to as much as 60 ng of...
676
pubmed23n0001_351
Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: a monitor of multiple conformational states in solution.
The red azoTyr-248-Zn complex of arnilazocarboxypeptidase, previously used to demonstrate differences in conformation of the enzyme in crystals and in solution, has now provided means to detect multiple conformations of the enzyme in solution by stopped-flow pH and temperature jump experiments. These studies identify t...
Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: a monitor of multiple conformational states in solution. The red azoTyr-248-Zn complex of arnilazocarboxypeptidase, previously used to demonstrate differences in conformation of the enzyme in crystals and in solution, has now provided means to dete...
677
pubmed23n0001_352
Presence of norepinephrine and related enzymes in isolated brain microvessels.
Norepinephrine and the enzymes involved in its synthesis and degradation were found to be associated with isolated brain microvessels. The significance of these results are discussed with respect to adrenergic innervation of the cerebral microvessels and thereby neural regulation of the cerebral microcirculation.
Presence of norepinephrine and related enzymes in isolated brain microvessels. Norepinephrine and the enzymes involved in its synthesis and degradation were found to be associated with isolated brain microvessels. The significance of these results are discussed with respect to adrenergic innervation of the cerebral mic...
678
pubmed23n0001_353
Disorders of micturition in bacterial prostatitis.
Measurements of urinary flow rate were performed in 16 patients with established prostatitis before and after a course of antimicrobial therapy. Before treatment the maximum flow rates were poor with abnormal flow curves and significant improvement in voiding characteristics were observed with treatment (P less than 0....
Disorders of micturition in bacterial prostatitis. Measurements of urinary flow rate were performed in 16 patients with established prostatitis before and after a course of antimicrobial therapy. Before treatment the maximum flow rates were poor with abnormal flow curves and significant improvement in voiding character...
681
pubmed23n0001_354
Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification.
The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme.
Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification. The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast rea...
698
pubmed23n0001_355
Relationship between bovine serum albumin structure and its chemical equilibria with hydrogen and 5-hydroxytryptamine ions.
From the analysis of titration curves with hydrogen and 5-HT ions, it was found that the electrostatic interaction parameter of protein macroion and the number sites of 5-HT fixing were smaller over an acid and base pH range as compared to their values at neutral pH. Our data were interpreted by the conformational chan...
Relationship between bovine serum albumin structure and its chemical equilibria with hydrogen and 5-hydroxytryptamine ions. From the analysis of titration curves with hydrogen and 5-HT ions, it was found that the electrostatic interaction parameter of protein macroion and the number sites of 5-HT fixing were smaller ov...
699
pubmed23n0001_356
Some psysicochemical properties of mitochondrial and cell sap alanine aminotransferase from the rat CNS.
The authors describe different properties of brain mitochondrial and cell sap alanine aminotransferase. They showed that the mitochondrial enzyme was inhibited by maleate, chlorides, acetate and phosphate with a high ionic strength (over 1.8), that its pH optimum lay between 7.5 and 8.5, that it was thermolabile at ove...
Some psysicochemical properties of mitochondrial and cell sap alanine aminotransferase from the rat CNS. The authors describe different properties of brain mitochondrial and cell sap alanine aminotransferase. They showed that the mitochondrial enzyme was inhibited by maleate, chlorides, acetate and phosphate with a hig...
701
pubmed23n0001_357
[Postvacccinal encephalomyelitis after protective antirabic innoculation, combined with polioencephalomyelitis (lyssa?)].
By means of two cases of postvaccinal encephalomyelitis following antirabies vaccination has been demonstrated the meaning of these complications. In one of these cases has been presumed a simultaneous illness of quiet rabies. The difficulty of an aetiological diagnostic by the morphological effigy of the encephalomyel...
[Postvacccinal encephalomyelitis after protective antirabic innoculation, combined with polioencephalomyelitis (lyssa?)]. By means of two cases of postvaccinal encephalomyelitis following antirabies vaccination has been demonstrated the meaning of these complications. In one of these cases has been presumed a simultane...
702
pubmed23n0001_358
The effect of L-dopa on young patients with simple schizophrenia, treated with neuroleptic drugs: a double-blind cross-over trial with Madopar and placebo.
Thirteen out of 18 young out-patients with simple schizophrenia under neuroleptic treatment completed a double-blind cross-over trial with Madopar [L-Dopa + benserazid (a peripheral decarboxylase inhibitor)] and placebo. Nine patients were given 900 mg L-Dopa + 225 mg benserazid daily, 1 patient received 600 mg L-Dopa ...
The effect of L-dopa on young patients with simple schizophrenia, treated with neuroleptic drugs: a double-blind cross-over trial with Madopar and placebo. Thirteen out of 18 young out-patients with simple schizophrenia under neuroleptic treatment completed a double-blind cross-over trial with Madopar [L-Dopa + bensera...
706
pubmed23n0001_359
The specificity of binding of the narcotic agonist etorphine in synaptic membranes of rat brain in vivo.
When 3H-etorphine was administered to rats in a pharmacologically effective dose (0.75 mug/kg intracisternally), the labeled drug was concentrated in synaptic membrane fractions isolated from the brains of rats killed 10 min after etorphine injection. Pretreatment of the animals with the narcotic antagonists naloxone, ...
The specificity of binding of the narcotic agonist etorphine in synaptic membranes of rat brain in vivo. When 3H-etorphine was administered to rats in a pharmacologically effective dose (0.75 mug/kg intracisternally), the labeled drug was concentrated in synaptic membrane fractions isolated from the brains of rats kill...
707
pubmed23n0001_360
The cholinergic system and nociception in the primate: interactions with morphine.
In Experiment 1 the shock titration task was used to evaluate the antinoceptive properties of 5 different classes of cholinergic compounds in the rhesus monkey. Only scopolamine and high doses of physostigmine were effective in elevating the shock threshold. The apparent antinociceptive effect of physostigmine, however...
The cholinergic system and nociception in the primate: interactions with morphine. In Experiment 1 the shock titration task was used to evaluate the antinoceptive properties of 5 different classes of cholinergic compounds in the rhesus monkey. Only scopolamine and high doses of physostigmine were effective in elevating...
708
pubmed23n0001_361
Effects of thienodiazepine derivatives on human sleep as compared to those of benzodiazepine derivatives.
The effects of new thienodiazepine derivatives, such as clotiazepam and Y-7131, on normal human sleep were investigated on 5 subjects and compared to those of benzodiazepine derivatives, such as diazepam and nitrazepam. REM sleep was significantly decreased only with 2 mg of Y-7131 and rebound elevation of REM sleep di...
Effects of thienodiazepine derivatives on human sleep as compared to those of benzodiazepine derivatives. The effects of new thienodiazepine derivatives, such as clotiazepam and Y-7131, on normal human sleep were investigated on 5 subjects and compared to those of benzodiazepine derivatives, such as diazepam and nitraz...
709
pubmed23n0001_362
Effect of two weeks' treatment with thioridazine, chlorpromazine, sulpiride and bromazepam, alone or in combination with alcohol, on learning and memory in man.
Forty paid healthy male students participated in two subacute experiments of 6 weeks each. In the first trial 20 of them received bromazepam, thioridazine, and placebo double blind cross over for 2 weeks each, and in the second trial the active agents administered to the other 20 participants were chlorpromazine and su...
Effect of two weeks' treatment with thioridazine, chlorpromazine, sulpiride and bromazepam, alone or in combination with alcohol, on learning and memory in man. Forty paid healthy male students participated in two subacute experiments of 6 weeks each. In the first trial 20 of them received bromazepam, thioridazine, and...
710
pubmed23n0001_363
Effects of chronic exposure to stressors on avoidance-escape behavior and on brain norepinephrine.
A single exposure to a severe stressor (either cold swim or inescapable shock) impairs subsequent performance in a shuttle avoidance-escape task (1), a deficit attributed to reduction in brain noradrenergic activity produced by these stressors. In the present paper, two experiments are described which examine how repea...
Effects of chronic exposure to stressors on avoidance-escape behavior and on brain norepinephrine. A single exposure to a severe stressor (either cold swim or inescapable shock) impairs subsequent performance in a shuttle avoidance-escape task (1), a deficit attributed to reduction in brain noradrenergic activity produ...
711
pubmed23n0001_364
Evaluation of combined pharmacological and psychotherapeutic treatment in patients with functional abdominal disorders.
78 patients suffering from various functional abdominal complaints have been trated in a 2 x 2 double-blind design: (a) psychotherapy with Ro 5-3350 (TH/Ro); (b) psychotherapy with placebo (TH/P); (c) Ro 5-3350 without psychotherapy (NIH/Ro); (d) placebo without psychotherapy (NTH/P). Results show that a considerable a...
Evaluation of combined pharmacological and psychotherapeutic treatment in patients with functional abdominal disorders. 78 patients suffering from various functional abdominal complaints have been trated in a 2 x 2 double-blind design: (a) psychotherapy with Ro 5-3350 (TH/Ro); (b) psychotherapy with placebo (TH/P); (c)...
725
pubmed23n0001_365
Role of cardiovascular and ionic changes in pathogenesis and prevention of isoprenaline-induced cardiac necrosis.
Blood pressure, heart rate, oxygen uptake, and blood values of PO2, PCO2, and pH were studied in unanesthetized rats for 8 hours. After a cardiotoxic dose of 20 mg/kg isoprenaline, s.c., blood pressure fell from 117 to 72 mm Hg, heart rate accelerated from 326 to 497 beats/minute, and cardiac work diminished by about 1...
Role of cardiovascular and ionic changes in pathogenesis and prevention of isoprenaline-induced cardiac necrosis. Blood pressure, heart rate, oxygen uptake, and blood values of PO2, PCO2, and pH were studied in unanesthetized rats for 8 hours. After a cardiotoxic dose of 20 mg/kg isoprenaline, s.c., blood pressure fell...
741
pubmed23n0001_366
Alterations in norepinephrine pattern in the damaged myocardium in the rat.
In the albino rat, the evolvement of myocardial necrosis induced by a single injection of ISO was accompanied by a fall in total NE. Pretreatment with propranolol and pargyline protected against ISO-induced necrosis and myocardial hypertrophy, but did not influence the ISO-induced depletion of NE stores. The depletion ...
Alterations in norepinephrine pattern in the damaged myocardium in the rat. In the albino rat, the evolvement of myocardial necrosis induced by a single injection of ISO was accompanied by a fall in total NE. Pretreatment with propranolol and pargyline protected against ISO-induced necrosis and myocardial hypertrophy, ...
742
pubmed23n0001_367
Prevention of myocardial Ca overload and necrotization by Mg and K salts or acidosis.
The crucial point in the pathogenesis of isoproterenol-induced myocardial necrotization is an abundant intracellular Ca accumulation leading to high energy phosphate exhaustion. Accordingly, in the early stage of the isoproterenol-induced necrotization process, the onset of ATP and creatine phosphate breakdown strictly...
Prevention of myocardial Ca overload and necrotization by Mg and K salts or acidosis. The crucial point in the pathogenesis of isoproterenol-induced myocardial necrotization is an abundant intracellular Ca accumulation leading to high energy phosphate exhaustion. Accordingly, in the early stage of the isoproterenol-ind...
743
pubmed23n0001_368
Explanation of the stimulation of microsomal N-demethylation reactions by soluble supernatant fraction.
Addition of the cell soluble supernatant fraction to an assay medium containing NADPH generating system, mixed function oxidase substrate and microsomes, resulted in a stimulation of drug metabolism ranging from 12-75%. This stimulation was observed only when the supply of DADPH generating system (isocitric dehydrogena...
Explanation of the stimulation of microsomal N-demethylation reactions by soluble supernatant fraction. Addition of the cell soluble supernatant fraction to an assay medium containing NADPH generating system, mixed function oxidase substrate and microsomes, resulted in a stimulation of drug metabolism ranging from 12-7...
745
pubmed23n0001_369
An attempt to correlated analgesia to changes in brain neuromediators in rats.
The analgesic activity of delta9THC, morphine and sodium salicylate was studied concomitantly with changes in brain stem levels of 5HT, 5HIAA, DA and NA. The results show that a correlation exists between analgesia and changes in the serotonergic system of the brain stem. Furthermore morphine sulfate was found to incre...
An attempt to correlated analgesia to changes in brain neuromediators in rats. The analgesic activity of delta9THC, morphine and sodium salicylate was studied concomitantly with changes in brain stem levels of 5HT, 5HIAA, DA and NA. The results show that a correlation exists between analgesia and changes in the seroton...
746
pubmed23n0001_370
Colorimetric determination of 5-aminosalicylic acid and its N-acetylated metabolite on urine and feces.
A simple and convenient colorimetric method is described for the quantitative determination of 5-aminosalicylic acid (5-ASA) and N-acetyl-5-ASA in urine and feces after oral administration of salicylazosulfa-pyridine (SASP), the drug of choice in the treatment of ulcerative colitis. N-acetyl-5-ASA is extracted directly...
Colorimetric determination of 5-aminosalicylic acid and its N-acetylated metabolite on urine and feces. A simple and convenient colorimetric method is described for the quantitative determination of 5-aminosalicylic acid (5-ASA) and N-acetyl-5-ASA in urine and feces after oral administration of salicylazosulfa-pyridine...
747
pubmed23n0001_371
[Pressures, blood gases, pH, lactate and pyruvate concentrations in the portal venous blood in patients after laparotomy during the first 9 days (author's transl)].
In 24 patients (16 women, 8 men, mean age 60 years), who underwent abdominal operations and who had a uncomplicated postoperative course, a teflon-tube was inserted into the portal vein at the end of laparotomy and remained there for maximal 9 days. The mean values of the portal venous pressure as well as of the portal...
[Pressures, blood gases, pH, lactate and pyruvate concentrations in the portal venous blood in patients after laparotomy during the first 9 days (author's transl)]. In 24 patients (16 women, 8 men, mean age 60 years), who underwent abdominal operations and who had a uncomplicated postoperative course, a teflon-tube was...
748
pubmed23n0001_372
Biochemical and biophysical changes in guinea pigs after acute head injury.
Animal experiments were set up mainly to derive additional diagnostic data from the study of biochemical changes after acute head injury. In standardized experiments guinea pigs were subjected in groups of 20 to three identical head injuries, each of either 1.0 J or 1.5 J intensity. The trauma was likely to result in a...
Biochemical and biophysical changes in guinea pigs after acute head injury. Animal experiments were set up mainly to derive additional diagnostic data from the study of biochemical changes after acute head injury. In standardized experiments guinea pigs were subjected in groups of 20 to three identical head injuries, e...
749
pubmed23n0001_373
Total brain ischaemia in dogs: cerebral physiological and metabolic changes after 15 minutes of circulatory arrest.
Cross-clamping of the ascending aorta in dogs for 15 min produced severe neurological deficit, observed for up to 20 h. Immediately after restoration of the circulation, the intracranial pressure in the cisterna magna increased transiently to a mean peak of 22.8 Torr (SD +/- 1.7) because of a compensatory increase in s...
Total brain ischaemia in dogs: cerebral physiological and metabolic changes after 15 minutes of circulatory arrest. Cross-clamping of the ascending aorta in dogs for 15 min produced severe neurological deficit, observed for up to 20 h. Immediately after restoration of the circulation, the intracranial pressure in the c...
750
pubmed23n0001_374
Oxygen and carbon dioxide dissociation of duck blood.
Oxygen and CO2 dissociation of duck blood was studied in blood samples equilibrated with known gas mixtures at the bird's body temperature (41 degrees C) and analyzed in the Van Slyke manometric apparatus and in pH electrodes. At various pH values between 7.38 and 7.55 the Hill plots yielded straight and parallel lines...
Oxygen and carbon dioxide dissociation of duck blood. Oxygen and CO2 dissociation of duck blood was studied in blood samples equilibrated with known gas mixtures at the bird's body temperature (41 degrees C) and analyzed in the Van Slyke manometric apparatus and in pH electrodes. At various pH values between 7.38 and 7...
751
pubmed23n0001_375
First apparent dissociation constant of carbonic acid, pK'1, of plasma and erythrocytes.
The first apparent dissociation constant of carbonic acid, pK'1, of plasma and red cells was determined on venous blood of ten healthy, adult, male, human subjects. pH and PCO2 of plasma and red cells were analyzed electrometrically and a micromanometric method was used for the determination of total carbon dioxide con...
First apparent dissociation constant of carbonic acid, pK'1, of plasma and erythrocytes. The first apparent dissociation constant of carbonic acid, pK'1, of plasma and red cells was determined on venous blood of ten healthy, adult, male, human subjects. pH and PCO2 of plasma and red cells were analyzed electrometricall...
752
pubmed23n0001_376
The effects of changes in pH and PCO2 in blood and water on breathing in rainbow trout, Salmo gairdneri.
The effect of sustained hypercapnia on the acid-base balance and gill ventilation in rainbow trout, Salmo gairdneri, was studied. The response to an increase in PICO2 from 0.3 to 5.2 mm Hg was a five-fold increase in gill ventilation volume and a slight increase in breathing frequency. There was a concomitant rise in P...
The effects of changes in pH and PCO2 in blood and water on breathing in rainbow trout, Salmo gairdneri. The effect of sustained hypercapnia on the acid-base balance and gill ventilation in rainbow trout, Salmo gairdneri, was studied. The response to an increase in PICO2 from 0.3 to 5.2 mm Hg was a five-fold increase i...
753
pubmed23n0001_377
The effect of pH upon fluoride uptake in intact enamel.
The relationship between pH and fluoride uptake in intact enamel of permanent premolars was investigated by using: (1) a sodium fluoride dentifrice, (2) a potassium fluoride + manganese chloride dentifrice, and (3) a sodium fluoride solution of the same fluoride concentration. The first part of this paper deals with th...
The effect of pH upon fluoride uptake in intact enamel. The relationship between pH and fluoride uptake in intact enamel of permanent premolars was investigated by using: (1) a sodium fluoride dentifrice, (2) a potassium fluoride + manganese chloride dentifrice, and (3) a sodium fluoride solution of the same fluoride c...
783
pubmed23n0001_378
Determination of inorganic pyrophosphatase in rat odontoblast layer by a radiochemical method.
The enzyme inorganic pyrophosphatase (PPiase, EC 3.6.1.1) from the odontoblastic layer of rat incisors has been studied by means of a radiochemical micromethod. The enzyme was incubated with 32P-pyrophosphate in tris-HCl buffer at 37 degrees C. The reaction was linear with time for at least 45 min, and the pH optimum w...
Determination of inorganic pyrophosphatase in rat odontoblast layer by a radiochemical method. The enzyme inorganic pyrophosphatase (PPiase, EC 3.6.1.1) from the odontoblastic layer of rat incisors has been studied by means of a radiochemical micromethod. The enzyme was incubated with 32P-pyrophosphate in tris-HCl buff...
784
pubmed23n0001_379
Assessment of denture plaque pH in subjects with and without denture stomatitis.
To evaluate the "resting" pH and induced pH changes in denture plaque, soft deposits were collected from the fitting surface of the denture, pooled and suspended in water. Plaque pH was determined with microelectrode equipment before and after mouth rinsing with a sucrose solution. A characteristic level in the "restin...
Assessment of denture plaque pH in subjects with and without denture stomatitis. To evaluate the "resting" pH and induced pH changes in denture plaque, soft deposits were collected from the fitting surface of the denture, pooled and suspended in water. Plaque pH was determined with microelectrode equipment before and a...
785
pubmed23n0001_380
Bioluminescence assay of enzymes obtained from buccal epithelium by superficial scraping.
A method is presented for the simultaneous assay of buccal enzymes by measuring reduced nicotinamide adenine dinucleotide and adenosine triphosphate with the aid of the bioluminescence of luciferase extracts. The activity of glucose-6-phosphate dehydrogenase (G6PDH) was shown earlier to be increased in homogeneous leuk...
Bioluminescence assay of enzymes obtained from buccal epithelium by superficial scraping. A method is presented for the simultaneous assay of buccal enzymes by measuring reduced nicotinamide adenine dinucleotide and adenosine triphosphate with the aid of the bioluminescence of luciferase extracts. The activity of gluco...
786
pubmed23n0001_381
Allogeneic marrow transplantation for the treatment of leukaemia. A review.
22 HL-A antigen and mixed leukocyte culture-matched sibling bone marrow transplants were attempted in patients with acute leukaemia (at the National Cancer Institute) to define the toxicities of four different immunosuppressive regimens, the complications associated with warrow engraftment and antileukaemic effect. 73%...
Allogeneic marrow transplantation for the treatment of leukaemia. A review. 22 HL-A antigen and mixed leukocyte culture-matched sibling bone marrow transplants were attempted in patients with acute leukaemia (at the National Cancer Institute) to define the toxicities of four different immunosuppressive regimens, the co...
789
pubmed23n0001_382
Current status of treatment of pneumonia.
Proper treatment of pneumonia is dependent upon a correct diagnosis. Pneumonia may be due to infectious agents, allergic phenomena, or chemical causes. Treatment regimens are outlined for the various types of pneumonia--pneumococcal, staphylococcal, fungal, and pneumonia due to gram-negative and anaerobic gram-negative...
Current status of treatment of pneumonia. Proper treatment of pneumonia is dependent upon a correct diagnosis. Pneumonia may be due to infectious agents, allergic phenomena, or chemical causes. Treatment regimens are outlined for the various types of pneumonia--pneumococcal, staphylococcal, fungal, and pneumonia due to...
792
pubmed23n0001_383
'Picture frame' fibres in a carrier of the trait for malignant hyperpyrexia.
A member of a family which was known to be susceptible to malignant hyperpyrexia, who was identified as a carrier by the presence of an elevated serum creatine-phosphokinase, has been investigated further. Muscle was examined biochemically, and the study included the sarcoplasmic ATPase-activity, actinomycin, Mg2+ ATPa...
'Picture frame' fibres in a carrier of the trait for malignant hyperpyrexia. A member of a family which was known to be susceptible to malignant hyperpyrexia, who was identified as a carrier by the presence of an elevated serum creatine-phosphokinase, has been investigated further. Muscle was examined biochemically, an...
795
pubmed23n0001_384
The GP dilemma. Recommendations and synopsis of a student conference.
The proceedings of a conference organised by students are reported. The present standing of the general practitioner and his need in different societies are equated and the obvious deficiencies are considered. Such themes as maldistribution, service and education are discussed. Resolutions derived from the conference a...
The GP dilemma. Recommendations and synopsis of a student conference. The proceedings of a conference organised by students are reported. The present standing of the general practitioner and his need in different societies are equated and the obvious deficiencies are considered. Such themes as maldistribution, service ...
796
pubmed23n0001_385
Gastric emptying of liquids after different vagotomies and pyloroplasty.
Gastric emptying of five liquid meals which differ in their physicochemical properties have been measured in control dogs and dogs that have received a Heinecke-Mikulicz pyloroplasty alone, proximal gastric vagotomy without drainage, selective gastric vagotomy and pyloroplasty and truncal vagotomy and pyloroplasty. The...
Gastric emptying of liquids after different vagotomies and pyloroplasty. Gastric emptying of five liquid meals which differ in their physicochemical properties have been measured in control dogs and dogs that have received a Heinecke-Mikulicz pyloroplasty alone, proximal gastric vagotomy without drainage, selective gas...
800
pubmed23n0001_386
Pancreatic enzyme response with an elemental diet.
Elemental diets can maintain or slightly improve the nutritional status without a major stimulatory effect on the pancreas. Six dogs were maintained with a regular chow diet, switched to an elemental diet and, subsequently, returned to a chow diet. Cannulation of the pancreatic duct through a duodenal cutaneous fistula...
Pancreatic enzyme response with an elemental diet. Elemental diets can maintain or slightly improve the nutritional status without a major stimulatory effect on the pancreas. Six dogs were maintained with a regular chow diet, switched to an elemental diet and, subsequently, returned to a chow diet. Cannulation of the p...
801
pubmed23n0001_387
Gastric fibrinolysis.
Gastric juice from 15 normals, 20 patients with gastric ulcer and 14 patients with erosive haemorrhagic gastroduodenitis was investigated in respect of its activity on unheated and heated fibrin plates and its content of FDP and plasminogen or plasmin with immunochemical methods. Gastric juice from normals showed no ac...
Gastric fibrinolysis. Gastric juice from 15 normals, 20 patients with gastric ulcer and 14 patients with erosive haemorrhagic gastroduodenitis was investigated in respect of its activity on unheated and heated fibrin plates and its content of FDP and plasminogen or plasmin with immunochemical methods. Gastric juice fro...
807
pubmed23n0001_388
Acid-base parameters in the dehydrated camel.
The effect of prolonged (10 days) dehydration on acid-base parameters of camel blood was examined. The pH and PCO2 levels rose significantly in the course of dehydration. This state was comparable with compensated non-respiratory alkalosis found in other animals. The plasma sodium, and magnesium levels rose significan...
Acid-base parameters in the dehydrated camel. The effect of prolonged (10 days) dehydration on acid-base parameters of camel blood was examined. The pH and PCO2 levels rose significantly in the course of dehydration. This state was comparable with compensated non-respiratory alkalosis found in other animals. The plasm...
814
pubmed23n0001_389
[Clinical experiences with untreated homologous vein grafts in reconstruction of arteries (author's transl)].
50 transplantations of homologous vein grafts in reconstruction of arteries are reported on. Vein grafts were either transplanted immediately or used after deep freezing. This procedure has proved to be effective in the replacement of arteries during our observation period of four years. Results of homologous vein tran...
[Clinical experiences with untreated homologous vein grafts in reconstruction of arteries (author's transl)]. 50 transplantations of homologous vein grafts in reconstruction of arteries are reported on. Vein grafts were either transplanted immediately or used after deep freezing. This procedure has proved to be effecti...
815
pubmed23n0001_390
The effect of a simulated subarachnoid hemorrhage on cerebral blood flow in the monkey.
The hydrogen clearance method was used to measure local and total cerebral blood flow (CBF) in the rhesus monkey before and for five hours after a simulated subarachnoid hemorrhage (SAH). CBF remained stable after SAH unless SAH was associated with a fall in cerebral perfusion pressure. In addition, cerebrovascular res...
The effect of a simulated subarachnoid hemorrhage on cerebral blood flow in the monkey. The hydrogen clearance method was used to measure local and total cerebral blood flow (CBF) in the rhesus monkey before and for five hours after a simulated subarachnoid hemorrhage (SAH). CBF remained stable after SAH unless SAH was...
820
pubmed23n0001_391
Effects of oxygen saturation and pCO2 on brain uptake of glucose analogues in rabbits.
The effect of oxygen saturation and PCO2 on brain uptake of glucose analogues was studied in rabbits. Using a modified Oldendorf technique, 14C-labeled glucose analogues with a 3H2O reference standard were introduced into the cerebral circulation via the common carotid artery, and the radioactivity of the ipsilateral c...
Effects of oxygen saturation and pCO2 on brain uptake of glucose analogues in rabbits. The effect of oxygen saturation and PCO2 on brain uptake of glucose analogues was studied in rabbits. Using a modified Oldendorf technique, 14C-labeled glucose analogues with a 3H2O reference standard were introduced into the cerebra...
821
pubmed23n0001_392
Engraftment of allogeneic dog bone marrow.
Resistance to allogeneic bone-marrow grafts (AR) was found to occur in many species, including the dog. The i.v. administration of silica particles suppressed AR in vivo in this species. Genetic studies provide suggestive evidence for the existence of a previously unrecognized system or systems in the canine major hist...
Engraftment of allogeneic dog bone marrow. Resistance to allogeneic bone-marrow grafts (AR) was found to occur in many species, including the dog. The i.v. administration of silica particles suppressed AR in vivo in this species. Genetic studies provide suggestive evidence for the existence of a previously unrecognized...
825
pubmed23n0001_393
Lactate and pyruvate concentrations, and acid-base balance of cerebrospinal fluid in experimentally induced intracerebral and subarachnoid hemorrhage in dogs.
The effect of blood injected into either subarachnoid space or subcortical brain tissue upon lactate and pyruvate concentrations as well as acid-base balance of cerebrospinal fluid (CSF) was studied in the anesthetized dog. CSF lactate and lactate/pyruvate ratio (L/P ratio) increased progressively following the intracr...
Lactate and pyruvate concentrations, and acid-base balance of cerebrospinal fluid in experimentally induced intracerebral and subarachnoid hemorrhage in dogs. The effect of blood injected into either subarachnoid space or subcortical brain tissue upon lactate and pyruvate concentrations as well as acid-base balance of ...
822
pubmed23n0001_394
A study of variables affecting the quality of platelets stored at "room temperature".
The effect of variables associated with the donor and with methods of collecting, processing, and storing platelets on the quality of platelets kept at ambient temperature was studied. Changes in structural integrity of platelets, decrease in pH, loss of aggregability, and kinetics in vivo of platelets tagged with 51Cr...
A study of variables affecting the quality of platelets stored at "room temperature". The effect of variables associated with the donor and with methods of collecting, processing, and storing platelets on the quality of platelets kept at ambient temperature was studied. Changes in structural integrity of platelets, dec...
823
pubmed23n0001_395
Dopamine-containing neurons of the substantia nigra and their terminals in the neostriatum.
Tne ultrastructural and fluorescence histochemical characteristics of the mature rabbit substantia nigra and neostriatum have been reviewed as a frame of reference for the developmental study. Biochemical investigations were reported on neostriatal dopamine concentrations and the relative uptake and accumulation of 3H-...
Dopamine-containing neurons of the substantia nigra and their terminals in the neostriatum. Tne ultrastructural and fluorescence histochemical characteristics of the mature rabbit substantia nigra and neostriatum have been reviewed as a frame of reference for the developmental study. Biochemical investigations were rep...
827
pubmed23n0001_396
[Separation of the hormones of the adenohypophysis of rats by use of electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate].
A comparative study of rat adenohypophysis extract and its alcohol fractions was performed by two variants of the method of electrophoresis in polyacrylamide gel: at pH 9,5 and with the presence of sodium dodecyl sulphate at pH 7.2. With the presence of sodium dodecylsulphate four protein zones are found which in the o...
[Separation of the hormones of the adenohypophysis of rats by use of electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate]. A comparative study of rat adenohypophysis extract and its alcohol fractions was performed by two variants of the method of electrophoresis in polyacrylamide gel: at pH ...
829
pubmed23n0001_397
[Properties of NAD-glycohydrolase of the nuclei of the liver cells of rats].
Certain properties of the rat liver cell nuclei NAD-glycohydrolase (EC 3.2.2.5) were investigated. It is established that its highest activity is at 37 degrees with activation energy equal to 9480 cal/M and with factor Q10 equal to 1.5. The enzyme pH optimum in 0.2 M tris acetate is equal to 6.5 and in 0.2 potassium ph...
[Properties of NAD-glycohydrolase of the nuclei of the liver cells of rats]. Certain properties of the rat liver cell nuclei NAD-glycohydrolase (EC 3.2.2.5) were investigated. It is established that its highest activity is at 37 degrees with activation energy equal to 9480 cal/M and with factor Q10 equal to 1.5. The en...
830
pubmed23n0001_398
[Some properties of "soluble" Na+ and K+-ATPase obtained from various subcellular membrane structures of the brain by use of non-ionic detergents].
A comparative study was carried out of some properties of "soluble" Na+, K+-ATPase obtained from different subcellular membrane brain structures by means of non-ionic detergents of triton X-100 and digitonin. It is established that temperature and pH-optima of "soluble" Na+, K+-ATPase are close to these optima of the i...
[Some properties of "soluble" Na+ and K+-ATPase obtained from various subcellular membrane structures of the brain by use of non-ionic detergents]. A comparative study was carried out of some properties of "soluble" Na+, K+-ATPase obtained from different subcellular membrane brain structures by means of non-ionic deter...
831
pubmed23n0001_399
[Properties of glutamine synthetase of the brain of rats during ontogenesis].
When investigating activity of glutamine synthetase of the enzymatic preparations isolated from the brain of rats of 0.5, 1, 3, 12 and 24-month age, no considerable differences were found in the indices of the values Km to a-glutamate and Vmax which are respectively equal to: Km (M-10(-3))=5.5; 3.5; 3.6; 3.9; 5.5; Vmax...
[Properties of glutamine synthetase of the brain of rats during ontogenesis]. When investigating activity of glutamine synthetase of the enzymatic preparations isolated from the brain of rats of 0.5, 1, 3, 12 and 24-month age, no considerable differences were found in the indices of the values Km to a-glutamate and Vma...
832