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8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is DNA polymerase? [proteins expressed by genome] | Kornberg discovered it catalyzes DNA synthesis adds deoxyribonucleotides to preexisting DNA template directed by the existing DNA template some remove mismatched pairings | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What does DNA replication require to take place? [proteins expressed by genome] | DNA n dNTP (of all 4 bases) 5' deoxynucleoside triphosphates Mg2+ free 3'OH primer | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] How does the template direct DNA replication? [proteins expressed by genome] | goes in a 5' -----> 3' direction 3' OH mounts a Nucleophilic attack on the alpha triphosphate chain attached to the 5' of the previous nucleotide PPi (pyrophosphate) is given off | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] describe the order in which a protein is made. [proteins expressed by genome] | DNA transcription to mRNA mRNA takes it to ribosome RNA translates with help from tRNA the codons into proteins | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe mRNA (E.coli) [proteins expressed by genome] | template with codons which serves as the master for translation. 1.2 kb long average for E coli 5% of total RNA (ribosomal RNA, tRNA make up rest) | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe tRNA [proteins expressed by genome] | one for each of 20 amino acids ~75 nucleotides ~25 kDa carries the activated amino acid and with its corresponding anticodon aids in translation in ribosome makes up about 15% of RNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe rRNA [proteins expressed by genome] | ribosomal RNA makes up 80% in E. coli This 80% is comprised of 23S, 16S and 5S species | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe snRNA [proteins expressed by genome] | only in Eukaryotes works in splicing out the inons | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe miRNA [proteins expressed by genome] | micro RNA class of small non-coding RNA's (~21 nucleotides These guys bind complementary mRNAs and therefore inhibit translation | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] describe small interfering RNA [proteins expressed by genome] | siRNA small non-coding RNA bind up mRNA and degrade it | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe RNA polymerases [proteins expressed by genome] | catalyzes transcription uses dsDNA or ssDNA as template makes and elongates RNA chains | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What does an RNA polymerase need to start elongation? [proteins expressed by genome] | ATP, GTP, UTP, CTP Mg2+ or Mn2+ template but NO primer needed | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe transcription [proteins expressed by genome] | RNA polymerase aids for the free 3'OH to mount nucleophilic attack on the incoming alpha phosphate reaction is aided by hydrolysis of PPi No proofreading here. The new mRNA strand is identical to the coding DNA strand. (not identical to template, but complementary to template) | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] How do mRNA in Eukaryotes differ from those in Prokaryotes? [proteins expressed by genome] | There is a 5' cap and a 3' poly AAAAAAAA structure added after transcription | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe the role and mechanism of tRNA [proteins expressed by genome] | brings amino acids to ribosome for making proteins aminoacyl tRNA synthetase uses ATP to make aminoacyl-tRNA which is a unique tRNA for each amino acid each one of these aminoacyl-tRNA's have a unique anticoding site which will recognize the codon on mRNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what is and what is the significance of a degenerate genetic code? [proteins expressed by genome] | amino acids can have more than one codon. 64 possible codons for 20 amino acids 3 triplets are stop codons so the leftover 61 code for the 20 amino acids degenerate code decreases harmful mutations and having too many chain terminators | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Where is the amino acid attached to tRNA? What's on the other end [proteins expressed by genome] | 3' end of tRNA attaches to the carboxyl carbon of the amino acid 5' end is phosphorolated | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Which two amino acids only have one codon? [proteins expressed by genome] | Trp Met | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Which three amino acids have 6 codons? [proteins expressed by genome] | Leu Ser Arg | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What are the stop codons? [proteins expressed by genome] | UAA UAG UGA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is the codon for Trp [proteins expressed by genome] | UGG Take a Trp to australia to get some uggs | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is the codon for Met? [proteins expressed by genome] | AUG Mett's birthday is in AUG | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] XY___ and XY____ ALWAYS encode for the same amino acid [proteins expressed by genome] | C and U | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] XY____ and XY_____ USUALLY code for same amino acid [proteins expressed by genome] | G and A | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] For E. coli what starts translation? [proteins expressed by genome] | On the mRNA chain a 5' shine dalgano (purine rich) marker occurs at -10, and then an AUG or GUG is +1 which tells the fMet to bind there and begin | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] For eukaryotes what starts translation? [proteins expressed by genome] | At the 5' cap, Met-tRNA binds to the first AUG it sees and goes from there. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] How does translation know when to stop? [proteins expressed by genome] | UAA, UAG and UGA are stop codons This binds protein which is not tRNA and it uses release factors to release the new protein | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe how universal the genetic code is. [proteins expressed by genome] | Nearly universal but some deviations in humans UGA is a code for Trp and not a stop codon instead we have AGA and AGG as stop codons and we don't code for Arg mitochondrial DNA is different because they make their own tRNA's 16 organisms deviate from the standard genetic code | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what are introns and exons [proteins expressed by genome] | introns are unused code and exons are expressed genes which means we must splice these guys. Humans have 8 introns which can be 50-10000 nucleotides long | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe human hemoglobin B chain with regard to introns and exons [proteins expressed by genome] | 3 exons and 2 introns | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe how we know about introns and exons? [proteins expressed by genome] | pre m RNA is heavier--has a 15S rate as opposed to the actual mRNA which is 9S rate. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is a spliceosome? [proteins expressed by genome] | assemblies of proteins and small RNA molecules recognizes that introns begin with GU and end with AG preceeded by essential pyrimidine tract | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What would be the benefit of exon shuffling or alternative splicing? [proteins expressed by genome] | shuffling allows for recombined genes which may provide diversity of function alternative splicing may lead to new proteins being formed | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] How do restriction enzymes provide the bases for gene exploration? [proteins expressed by genome] | restriction endonucleases cut DNA in certain spots where they recognize specific sequences and cut both strands | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Where do restriction enzymes know where to cut? [proteins expressed by genome] | the cleavage site is 4-8 bp at a palindromic symmetry site. They see this symmetry and hydrolyze the phosphodiester bonds here can leave staggered (sticky) or even (blunt) cuts. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] EcoRI [proteins expressed by genome] | 5' G*AATTC 3' 3' CTTAA*G 3' derived from E. coli | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What are the endogenous and laboratory uses for restriction endonucleases [proteins expressed by genome] | In cells, these guys cleave foriegn DNA without harming host DNA In labs, we use it to cleave DNA into fingerprints so we can work with it better | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe SV40 [proteins expressed by genome] | 5.1 kb circular DNA has 1 EcoRI cleavage sit has 4 HpaI has 11 HindIII | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Once you have DNA fragments what do you do with them to manipulate them? [proteins expressed by genome] | Gel electrophoresis will separate up to 1,000bp (more porous agarose up to 20 kb) Pulse Field Gel Elect. separates chromosomes | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what does ethidium bromide do? [proteins expressed by genome] | stains dsDNA fragments after separated by gel elect. and has sensitivity to show 50ng with orange fluorescence | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Explain how to identify restriction fragments using 32P probe which technique is this? [proteins expressed by genome] | Separate different fragments in gel denature them to make ssDNA use a nitrocellulose sheet a strand, matching the one you are looking for has 32P incorporated. when it finds its ppair it links up and audioradiography will show you which one it is on paper southern blotting | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What other things can southern blots reveal about a restriction fragment? [proteins expressed by genome] | mutations on restriction fragment grounds for polymorphism RFLP restriction fragment length polymorphism | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is northern blotting? [proteins expressed by genome] | same technique as southern but for RNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe dideoxy method for DNA sequencing [proteins expressed by genome] | The method of incorporating a small amount of dideoxynucleoside triphosphatees which serve to effectively terminate replication at various points primed with synthesized fragment use DNA polymerase add all the deoxyribonucleoside triphosphates and 2' 3' dideoxy gel and audioradiogram to sequence | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what's a more modern less radioactive sanger method? [proteins expressed by genome] | fluorescence tagging can run all four bases in one reaction up to 500 bases can be determined automated. can generate more than 1 million bases / day | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What method is used for making DNA or DNA probes? [proteins expressed by genome] | solid phase phosphite triester method use glass bead add excess reagents to drive rxn to completion sequential addition of protonated (proteceted) deoxyribonucleoside 3' phosphoramidites wash soluable protectors away | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what are the reagents for phosphite triester method? [proteins expressed by genome] | deoxynucleoside 3' phosphoramidite DMT dimethoxytrityl BCE B cyanoethyl I2 (oxidizer) dicholoroacetic acid NH3 | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is the role of NH3 in phosphite triester method? [proteins expressed by genome] | remove all protecting groups and remove the deoxyribonucleoside 3' phosphoramidite from the glass bead | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what is role of dichloroacetic acid in phosphite triester method? [proteins expressed by genome] | removes DMT from 5' end of newly added deoxyribonucleoside. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] where does the triester form in phosphite diester method on activated monomer and chain? [proteins expressed by genome] | phosphit on 3' of activated monomer attaches to 5 ' OH of chain. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What's the efficiency of the phosphite triester method? [proteins expressed by genome] | 99% 10 min per cycle elongation is never 100% so the longest one is the golden one. You can find this using PAGE or HPLC | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Once you makes some DNA using the phosphite triester method, what can you do with it? [proteins expressed by genome] | make probes (32P or fluorescence tag and then set it loose in fragments to find the needles in the haystack) use it as a primer for new neighboring DNA make new designer genes which can make new funcitons or new proteins | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is PCR? [proteins expressed by genome] | Polymerase Chain Reaction amplifies your sample | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What are the 3 steps in PCR> [proteins expressed by genome] | strand separation (heat) hybridize primers (cool with a ton of primers in there --20-30 nucleotides long) heat to 72 so Taq polymerase can start making the strand 5' -3' repeat 25-30 times n cycles = 2tothe n results | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe recombinant DNA technology [proteins expressed by genome] | DNA fragment + a vector (plasmids or lambda phage) host (E. coli) | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe how Eco RI works with plasmid pSC101 [proteins expressed by genome] | cuts pSC101 at a specific site leaving a sticky end. If you use Eco RI to cut any piece of inserting DNA those same sticky ends will line up and voila. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is DNA ligase? [proteins expressed by genome] | DNA ligase catalyzes the formation of phosphodiester bonds needs a free 3'OH and 5' phosphate as well as ATP or NAD+ | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe the cohesive ends (Linkers) method. [proteins expressed by genome] | If you have a DNA segment you can add cohesive ends to put the tape on the ends. polynucleotide kinase phosphorylates the 5' end of a linker T4phage ligase seals them (can be blunt ended). then use EcoRI to make the sticky ends and insert into your vector | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what is a polylinker? [proteins expressed by genome] | a synthetic DNA fragment programed with several sequences that many restriction endonucleases recognize. Can custom make your sticky ends. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] why are plasmids and lambda phages good vectors? [proteins expressed by genome] | they get the recombinant DNA into bacteria easily which you can then select for. Plasmids carry genes for inactivation of antibiotics and can replicate independently of host genome | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is it about pBR322 that makes it good for cloning? [proteins expressed by genome] | has genes for resistance to tetracycline and ampicilin the EcoRI site isn't in these genes for the ab resistance but it does have other restriction enzyme sites which will interrupt the resistance | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe selection using pBR322 [proteins expressed by genome] | pBR322 uses the enzyme cut in amp. resistant element and foreign DNA ligated in. Now these guys are going to be amp sensitive. Still resistant to tetra. grow E coli on plates of tetra. transferred in matching postions to new plates . One plate has tetra and another has amp and tetra. Now you will know which ones are am... | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What size is a plasmid? [proteins expressed by genome] | 2-100+ kb | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is the size of lambda phage? [proteins expressed by genome] | 48 kb | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What are the differences between a lytic and lysogenic pathway? [proteins expressed by genome] | lytic kills host cell quickly and produces ~100 virons lysogenic inserts DNA into host cell genome and it is replicated for many generations Upon activation it expresses dormant viral DNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is lambda gt-lambdaB [proteins expressed by genome] | mutant lambda phage with only 2 EcoRI sites as opposed to the normal 5 enter bacteria way easier than plasmid | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe lambda gt-lambdaB and how it gets packaged. [proteins expressed by genome] | EcoRI cleavage gives two arms, and foreign DNA inserted to make it 93% (fits in the 75-105% size requirements | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what is cosmid lambda? [proteins expressed by genome] | hybrid of lambda phage and a plasmid accomodates large DNA inserts of 45 kb!! | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is a BAC [proteins expressed by genome] | Bacterial artificial chromosome engineered version of ecoli fertility F factor. accomodates inserts of DNA in 100,000-300,000 bp selectable with chloramphenicol resistance put in bacteria by electroporation | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is a YAC [proteins expressed by genome] | Yeast Artificial Chromosome accomodates inserts as big as 2,000,000 bp lots of stuff in em that we didn't really cover but that makes them ideal for big genomes | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is a genomic digest? [proteins expressed by genome] | mechanically sheared fragments of a 3,000,000 kb genome makes random overlapping fragments which can be PAGEd isolate 15 kb long molecules and add linkers and then insert into lambda phages and then grown in E coli. gives you genomic library | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Why do we need DNA hybridization? [proteins expressed by genome] | Allows us to screen the genomic library | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is DNA hybridization? [proteins expressed by genome] | the recombinant phages are plated on a lawn of bacteria where they like guys grow a plaque. Nitrocellulose is then applied and we add NaOH to lyse and denature the DNA. 32P probe DNA is introduced and the target sequence is radioaudiographed. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] How do we know how to make the probe for human hybridization screening? [proteins expressed by genome] | take the mRNA from an enriched source of what you are looking for. Make a cDNA from this mRNA and use as probe use amino acid sequence from target (use sequences with a lot of Trp and Met Synthesize the probes with 32P Use all the probes and isolate and sequence positive clones | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Why do we need a probe with minimal degeneracy? [proteins expressed by genome] | lots of peptides are encoded by several oligonucleotides. Sequences where there is alot of Met or Trp are best because they only have one codon | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Why isn't Ecoli. good for mammalian mRNA? [proteins expressed by genome] | E. coli doesn't have the ability to splice, so we have to make cDNA from mRNA (which doesn't have introns) | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What is the difference between SINES and LINES? [proteins expressed by genome] | Short intersperced elements (DNA that doesn't code for proteins) 1,000,000 Alu sequences of 300bp Long intersperced elements nearly 1 million LINES up to 10 kbp | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] how do we make cDNAs [proteins expressed by genome] | mRNA + oligo T primer with 3'OH + 4 dNTP's oligo T pairs with poly A at 3' end of mRNA reverse transcriptase makes a DNA complementary to RNA template increase the pH and dissolve mRNA add terminal transferase which adds oligo GGG to 3' end of the ssDNA then add oligo CCCC to make cDNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] What antibody do we use in immunochemical screening of cloned cDNA? [proteins expressed by genome] | 125I antibody | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] Describe DNA microinjection method/ [proteins expressed by genome] | 0.1 mm micropipet yeilds 2% viability foster mother mouse | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
8b8294df_r_3_Berg_Flashcards___Cram_com__proteins_expressed_by_genome | [proteome] what is GH 21-kd protein? [proteins expressed by genome] | rat growth hormone in rats it is promoted by metallothionein promoter have an activator | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteins expressed by genome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Mild cases- artificial tears Moderate to severe- Elevate head of bed –reduce congestion Oral prednisone Orbital radiation Orbital decompression [Cataract] | Thyroid eye disease | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | The diabetic control and complications trial has shown that tight glycemic control decreases the progression of diabetic retinopathy, nephropathy, and neuropathy Prompt referral indicated Laser photocoagulation [Cataract] | Diabetic retinopathy | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Tight glycemic control Refer Laser photocoagulation to decrease further decline in visual acuity by reducing amount of macular edema (ETDRS) [Cataract] | Diabetic macular degeneration | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treat the underlying hypertension Treat the systemic vascular disease or renal disorders Chronic does not require specific ophthalmic treatment [Cataract] | Hypertensive retinopathy | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Mandatory ophthalmic exam within 48-72 hours of diagnosis BRVO with macular edema may result in decreased vision-laser photocoagulation Follow up- crvo assessed every month for 6 months by the eye doc Visual prognosis depends on severity [Cataract] | Retinal Vein occlusion | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Acute vision changes-refer within 24 hours Laser photocoagulation Subretinal surgery experimental Low vision aids-magnification Preservision vitamins have been shown to improve patients with moderate to severe AMD Visudyne – a new procedure for wet AMD [Cataract] | age related macular degeneration | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment: remove debris, antibiotic and steroid drops, keep dry Acidifying agent +/- hydrocortisone Neomycin/polymyxin-B/hydrocortisone Fluoroquinolone +/- dexamethasone Perforated TM: Floxin otic + PO quinolone Wick insertion [Cataract] | Otitis Externa | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment: Antibiotics: Moderate to severe otalgia Fever > 39c (102.2f) Less than 2 years of age Observation: Improvement: 61% in 24h, 75% in 1 week Antibiotics: First line: amoxicillin (macrolide) Second line: amox/clavulanate, ceph2 PCN allergic: macrolide Auralgan: benzocaine drops Tympanocentesis [Cataract] | Acute otitis media | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment options: Antibiotics: first line, limited benefit Antihistamines/decongestants: no benefit Steroids: not recommended Ventilating tubes: initial benefit, limited by one year. No proven effects on language Adenoidectomy: older than 4 y.o. [Cataract] | Otitis media with effusion | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment: Antibiotics: fluoroquinolones Surgery [Cataract] | Chronic Otitis media | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Hearing aid, cochlear implant [Cataract] | Presbyacusis | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment: surgery, radiotherapy [Cataract] | Acoustic neuroma | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment: Low salt diet, limit alcohol and caffeine Diuretics, antiemetics, anxiolytics (alprazolam) Surgery [Cataract] | Menieres disease | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment: Reverse underlying cause Treat associated insomnia and depression [Cataract] | Tinnitus | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | anticholinergics, antiemetics, benzodiazepines [Cataract] | Vertigo | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment: Antihistamines, decongestants, guaifenesin Antitussives Ipratropium, cromolyn Zinc Vitamin C: no benefit, may help prevent Echinacea: no benefit [Cataract] | Viral URI | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment First line antibiotics: 7-10 days Amoxicillin Amoxicillin-clavulanate Trimethoprim-sulfamethoxasole Clarithromycin Doxycycline Second line antibiotics: 7-28 days 2nd generation cephalosporin (cefuroxime) Fluoroquinolone (levofloxacin) Treatment: Intranasal steroids: no data Surgery: endoscopic [Cataract] | Acute Sinusitis | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment: Medications: Antibiotics (3-6 weeks) Intranasal steroid sprays Guaifenesin Antihistamines Nasal irrigation Antibiotics: First line: amox-clavulanate, ceph2 PCN allergic: clarithromycin, clindamycin Second line: levofloxacin, moxifloxacin Functional endoscopic surgery (FESS) [Cataract] | Chronic sinusitis | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
310113b3_Language_Flashcards___Cram_com__Cataract | Treatment Avoidance: dust, mold, dander, pollen Medications: Intranasal steroids (fluticasone) Antihistamines (first and second generation) Decongestants Leukotriene inhibitors (montelukast) Mast cell stabilizer sprays (nedrocromil) Ipratropium bromide nasal spray: rhinorrhea Allergen injection immunotherapy [Cataract]... | Allergic Rhinitis | [] | Clin Med exam 1 treatments Foreign Language Flashcards - Cram.com | Cataract | http://www.cram.com/flashcards/clin-med-exam-1-treatments-861452 | 29/1438042987628.47_20150728002307-00054-ip-10-236-191-2_375950659_0.json |
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