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d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] TRICARE Prime. [Question]
The health maintenance organization provided for dependents of active duty military personnel is called
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] CHAMPVA beneficiaries. [Question]
People NOT entitled to benefits under TRICARE are
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] TRI-CHECK [Question]
What is the system called that TRICARE claims processors use to verify beneficiary eligibility?
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] certification from a military hospital stating that it cannot provide the necessary care. [Question]
An NAS is a/an
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] Both a and c [Question]
The NAS catchment area is
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] cooperative care. [Question]
Medical care that is cost-shared by both TRICARE Standard and a civilian source is known as
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] October 1 to September 30. [Question]
The TRICARE fiscal year extends from
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] All of the above. [Question]
Health care professionals who may treat a TRICARE patient are
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] HCF. [Question]
A health care professional, usually a registered nurse, who helps the patient work with his or her primary care manager to locate a specialist or obtain a preauthorization for care is referred to as a/an
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] no more than 115% of the TRICARE allowable charge. [Question]
A physician who chooses not to participate in TRICARE bills
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] 1 year at a time. [Question]
enrollment in TRICARE Prime is for
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] PCM. [Question]
The physician who is responsible for coordinating and managing all of the health care for the TRICARE Prime patient is referred to as a/an
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] service/health benefit prgrams. [Question]
TRICARE Standard and CHAMPVA are
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] CHAMPVA program. [Question]
The Veterans Health Care Expansion Act of 1973 authorized the
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] Both a and b [Question]
The wife of a veteran with total permanent disability resulting from a service-connected injury is eligible for CHAMPVA benefits
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] All of the above [Question]
The Privacy Act of 1974 establishes an individual's right to review his or her medical records maintained by a
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] within 1 year from the dte a service is provided [Question]
The time limit within which a TRICARE outpatient claim must be filed is
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] 1 year from a patient's discharge from an inpatient facility. [Question]
The time limit within which a TRICARE inpatient claim must be filed is within
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] filed by the provider to a TRICARE subcontractor. [Question]
TRICARE Prime and TRICARE Extra claims are
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] individual time limits for each item on the claim. [Question]
If a TRICARE Extra claim is submitted with several items and several dates of service, the time limit that would apply to the claim for filing would be
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] nonavailability statement (NAS) [Question]
A certification from a military hospital stating that it connot provide the care needed is called a ______________________
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] 10 [Question]
All dependents _________ years of age or oder are required to have a Uniformed Services (military) identification card.
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
d2d73e7f_Flashcards_about_Fordney_14__Question
[Answer] network. [Question]
The physician who provides medical care at contracted rates to beneficiaries under the TRICARE Extra program is called a/an ________________ provider.
[]
Flashcards about Fordney 14
Question
http://www.studystack.com/flashcard-41278
22/1438042990609.0_20150728002310-00045-ip-10-236-191-2_745874149_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] Mistreatment, in any form, of older people. [Question]
elder abuse
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] Not taking proper care of someone in your care; this could be a child, an elderly person, or even a spouse. [Question]
neglect
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] mistreatment of a child of a sexual, physical, or emotional nature; battering; beating; hurting. [Question]
child abuse
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] this occurs when someone uses another person for sexual reasons; it is usually an adult who uses a child this way, but not always [Question]
sexual abuse
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] mistreating a person you are married to; the meaning has been enlarged to include boyfriends and girlfriends; it is also generally called battering. [Question]
spouse abuse
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] sexual relationship with a member of the family. [Question]
incest
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] government office that looks after the health or well-being of children. [Question]
child welfare agency
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] care from someone other than the natural parents. [Question]
foster parenting
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] a court order to stop a particular activity, such as harassment. [Question]
injunction
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] toll-free telephone number people can call for assistance of a certain kind. [Question]
hotline
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] when a criminal has to repay the victim as part of his/her sentence. [Question]
Restitution
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] when the government helps a victim replace his/her losses due to a crime [Question]
compensation
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
c188e841_rds_about_Ms_Joyner___fam__law__Question
[Answer] a law in CA that states a woman's history of abuse can be considered at trial when she has committed a crime against her abuser. [Question]
The Habeas Law
[]
Flashcards about Ms Joyner - fam. law
Question
http://www.studystack.com/flashcard-812032
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] the mental activities associated with thinking, knowing, remembering, and communicating. [Question]
cognition
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] a mental grouping of similar objects, events, ideas, or people. [Question]
concept
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] a mental image or best example of a category. Matching new items to a prototype provides a quick and easy method for sorting items into categories [Question]
prototype
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] a methodical, logical rule or procedure that guarantees solving a particular problem. Contrast with the usually speedier-but also more error-prone-use of heuristics. [Question]
algorithm
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] a simple thinking strategy that often allows us to make judgements and solve problems efficiently; usually speedier but also more error-prone than algorithms. [Question]
heuristic
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] a sudden and often novel realization of the solution to a problem; it contrasts with strategy-based solutions. [Question]
insight
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] a tendency to search for information that supports our preconceptions and to ignore or distort contradictory evidence. [Question]
confirmation bias
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] the inability to see a problem from a new perspective, by employing a different mental set. [Question]
fixation
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] a tendency to approach a problem in one particular way, often a way that has been successful in the past. [Question]
mental set
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] the tendency to think of things only in terms of their usual functions; an impediment to problem solving. [Question]
functional fixedness
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] judging the likelihood of things in terms of how well they seem to represent, or match, particular prototypes; may lead us to ignore other relevant information. [Question]
representativeness heuristic
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] estimating the likelihood of events based on their availability in memory; if instances come readily to mind (perhaps because of their vividness), we presume such events are common. [Question]
availability heuristic
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] the tendency to be more confident than correct-to overestimate the accuracy of our beliefs and judgements. [Question]
overconfidence
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] clinging to one's initial conceptions after the basis on which they were formed has been discredited. [Question]
belief perseverance
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] an effortless, immediate, automatic feeling or thought, as contrasted with explicit, conscious reasoning. [Question]
intuition
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] the way an issue is posed; how an issue is framed can significantly affect decisions and judgements. [Question]
framing
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] our spoken, written, or signed words and the ways we combine them to communicate meaning. [Question]
language
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] in language, the smallest distinctive sound unit. [Question]
phoneme
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] in a language, the smallest unit that carries meaning; may be a word or a part of a word. [Question]
morpheme
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] in a language, a system of rules that enables us to communicate with and understand others. [Question]
grammar
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] the set of rules by which we derive meaning from morphemes, words, and sentences in a given language; also, the study of meaning. [Question]
semantics
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] the rules for combining words into grammatically sensible sentences in a given language. [Question]
syntax
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] beginning at about 4 months, the stage of speech development in which the infant spontaneously utters various sounds at first unrelated to the household language. [Question]
babbling stage
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] the stage in speech development, from about age 1 to 2, during which a child speaks mostly in single words. [Question]
one-word stage
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] beginning about age 2, the stage in speech development during which a child speaks mostly two-word statements. [Question]
two-word stage
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] early speech stage in which a child speaks like a telegram-"go car"- using mostly nouns and verbs. [Question]
telegraphic speech
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
22/1438043062635.98_20150728002422-00230-ip-10-236-191-2_733581263_3.json
6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] impairment of language, usually caused by left hemisphere damage either to Broca's area (impairing speaking) or to Wernicke's area (impairing understanding) [Question]
aphasia
[]
Flashcards about Myers 9 Chapter 9
Question
http://www.studystack.com/flashcard-715587
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6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] control language expression-an area of the frontal lobe, uaually in the left hemisphere, that directs the muscle movements involved in speech. [Question]
Broca's area
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6da7949b_hcards_about_Myers_9_Chapter_9__Question
[Answer] controls language reception-a brain area involved in language comprehension and expression; usually in the left temporal lobe. [Question]
Wernicke's area
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[Answer] Whorf's hypothesis that language determines the way we think. [Question]
linguistic determinism
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] What is fluorescence? [Answer]
The process by which light is absorbed by a molecule and then re emitted at a longer wavelength, producing a particular color.
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[Question] What is a fluorophore? [Answer]
the chemical structure in a fluorescent molecule that absorbs and emits the photons of light.
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] EGFP [Answer]
enhanced green fluorescent protein. has increased fluorescent light output. 35x more brightly. Ser64Thr
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Flashcards about UCSD BIBC3 Exam 3
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] ECFP [Answer]
enhanced cyan fluorescent protein. changed the color from green to cyan.
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] EYFP [Answer]
enhanced yellow FP. changed color to yellow.
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Flashcards about UCSD BIBC3 Exam 3
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] DsRed [Answer]
is from the coral Discosoma. It is a tetramer formed of 4 identical subunits of 26kDa.
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] The 2 parent proteins [Answer]
GFP and DsRed
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[Question] FP genes were cloned into the plasmid vector... [Answer]
pRSET in the host bacterium E.Coli strain DH5alpha.
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] plasmids [Answer]
are circular double stranded DNA mol'cs capable of autonomous replication w/in host bacterium. they are not part of the bacterial chromosome.
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[Question] vectors [Answer]
are a vehicle for maintaining and propagating cloned genes in a host organism, such as E.coli. foreign genes can by inserted into a plasmid vector and are rep'd along w/plasmid DNA inside bact. cell.
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] cloning [Answer]
the production of multiple identical copies of an organism, cell or gene. if a gene is isolated from genetic material of an organism, and inserted into a vector where identical copies can be rep'd, it is referred to as cloned.
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[Question] PT7 [Answer]
bacteriophage T7 promotor. This sequence is the binding site for T7 RNA polymerase that will synthesize the mRNA.
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[Question] RBS [Answer]
ribosome binding site. Sequence that binds the mRNA to the ribosome.
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[Question] ATG [Answer]
vector start codon. This codon codes for methionine, and is the signal for the ribosome to initiate synthesis of the AA chain.
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
[Question] 6xHis [Answer]
His-tag. This sequence encodes 6 histidine aa acids in tandem. These will bind to a nickel aff. column for fast and efficient purification of the protein encoded on the cloned gene.
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[Question] Xpress Epitope Tag [Answer]
8 aa sequence(DLYDDDDK). that is recognized by an antibody. This provides an alternative method for purifying(and detecting) the protein by aff. chromatography.
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[Question] EK [Answer]
aa seq. that is cut by the protease enterokinase. it can be used to remove the preceding aa(met,6 his, DLYDDDDK) that are encoded by the vector.
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[Question] MCS [Answer]
multiple cloning site. a series of tandem restr.enzy. cutting sites.(BAMHI-HindIII).this is where the gene encoding the protein of interest (DNA frag. containing pRSET and gene) is inserted into vector.
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[Question] Stop [Answer]
vector translation stop. The stop codon TAA terminates translation from the mRNA.
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[Question] f1 ori [Answer]
origin of rep. for f1 bacteriophage of e.coli. used in connection w/helper phage(which provides all necessary f1 gene products) to produce single str. copies of the plasmid w/cloned genes(useful for DNA seq.ing of the cloned gene)
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[Question] Ampicillin(BLA gene) [Answer]
the BLA gene that encodes beta-lactamase, the enzy. that degrades the antibiotic ampicillin. In the presence of ampicillin, only bact. containing a plasmid w/amp-resistance gene will live.
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[Question] Ampicillin [Answer]
inhibits the enzymes that form peptide cross-links btwn carbohydrate chains and bacterial cell wall. This weakens the cell wall and lysis eventually occurs.
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[Question] pUC [Answer]
an E.coli replication initiation site is inserted here. The DNA polymerases of the host bacterium bind to this site to replicate the vector DNA.
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[Question] Where were the genes for FP's cloned to? [Answer]
The MCS of pRSET-B. BAMHI is the site cut by the restriction enzyme BAMHI, where the 5' end of the FP gene was insterted.EcoR1 is the site where the 3' end of the gene was inserted.
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[Question] The role of glucose in Solution one of alkaline lysis mini plasmid prep? [Answer]
to increase the [solute] outside of the cell so when sol.II is added, osmotic pressure draws H2O out hopefully carrying the plasmid.
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[Question] The role of EDTA in solution I of the alkaline lysis mini plasmid prep? [Answer]
EDTA is a chelator. It's main function is to remove Mg2+ and inactivate DNA nucleases for which this ion is cofactor.
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[Question] The role of RNase in solution I of the alkaline lysis mini plasmid prep? [Answer]
It is a ribonuclease that will degrade contaminating bacterial RNA.
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[Question] The role of NaOH in solution II? [Answer]
To raise the pH, which causes the DNA to denature and the separate strands will ppt from solution.
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[Question] The role of 1% SDS Page in solution II? [Answer]
to lyse the cells by dissolving the cell membrane.
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[Question] The role of Kacetate in solution III? [Answer]
It makes the solution more neutral. Causes plasmid DNA strands to re-anneal. K+ ions comples w/dodecyl sulfate ions and ppt.
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[Question] Why doesn't the chromosomal DNA re-anneal in solution III? [Answer]
It is too big and complex to re-anneal under those conditions.
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[Question] The role of Isopropanol? [Answer]
causes the plasmid DNA to ppt.
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[Question] The role of ethanol wash? [Answer]
to remove remaining salts and detergents.
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Flashcards about UCSD BIBC3 Exam 3
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[Question] Purity of Plasmid DNA [Answer]
Is measured by the ratio of A260/A280. Greater than 1.6 is adequate. Greater than 1.8 is better. Protein contamination reduces the ratio.
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