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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Nucleotides absorb UV light with a maximum at? [Answer]
|
260nm
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] The purpose of an alcohol precipitation is to? [Answer]
|
1. concentrate DNA or RNA and/or 2. remove detergents, salts and other low MW contaminants. or to change buffer composition.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] nucleic acids will not precipitate unless... [Answer]
|
there is a monovalent cation to neutralize the negative charges and permit DNA to aggregate.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] NH4acetate is preferred when one wants to remove.. [Answer]
|
free deoxynucleotides, as they co-precipitate less with NH4 than Na.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] LiCl is commonly used to ppt [Answer]
|
RNA, b/c it is more soluble in ethanol than Na, and efficient ppt of RNA requires large amounts of ethanol than DNA.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] When ppting nucleic acids in the presence of SDS, why use K+? [Answer]
|
K+ is used when you want the SDS to ppt as well. SDS is soluble, and KDS or potassium dodecyl sulfate is is insoluble.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Why is water an excellent solvent for oppositely charged molecules? [Answer]
|
Since the polar water molecules orient in concentric shells around the charges, forming hydration spheres. This shields there charge so the attractive forces btwn them is less and they don't come together to form a ppt.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] hydration sphere [Answer]
|
separates charges so the attractive forces btwn them is less and they don't come together and form a ppt.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] When alcohol is added to solution, solubility is decreased for two reasons: [Answer]
|
1.alcohols form Hbonds with water, which reduces the amt of water available to dissolve 2. the capacity of alcohols to shield opp charges and keep them apart is much less than water.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] restriction enzymes [Answer]
|
are nucleases that are produced by bacteria to protect them from viruses that infect bacterial cells, called bacteriophage. They recognize a spec. seq of nucleotides in bacteriophage chrom. and produce a dbl str cut in DNA to prevent replication.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] How does the bacterium protect its own DNA from being cut by restriction enzymes? [Answer]
|
By methylating adenosine or cytidine nucleotide in the sequence, which prevents endonuclease from binding.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] How are restriction enzymes named? [Answer]
|
with the first letter of the genus, followed by the first 2 letters of the species, followed by the strain or serotype if any, and a roman numeral if the bacterium contains more than 1type of res. enzy.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Why does DNA have a uniformly negative charge? [Answer]
|
This is due to the acidic phosphodiaster groups.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Supercoiled DNA will migrate slower or faster than liner DNA on agarose gel electrophoresis? [Answer]
|
it migrates faster b/c it is more compact. plasmid dna is normally supercoiled
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] If DNA is nicked, how will this affect its migration down a gel? [Answer]
|
dna uncoils, but remains circular and usually migrates more slowly than linear dna of the same size
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] The 2 most common buffer systems for agarose gel electrophoresis are: [Answer]
|
Tris-acetate EDTA (TAE) and Tris-borate-EDTA (TBE).
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] What is the difference between using TAE and TBE as a buffer system? [Answer]
|
TAE gives a better resolution and is preferred when DNA is to be purified from the gel afterwards. TBE has a better buffering capacity.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] What dye was used to track the proteins in the electrophoresis gel? [Answer]
|
bromophenol blue. it will run at about the same position as 300bp
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Syber safe [Answer]
|
a fluorescent dye used in our electro gel to help visualize dna. it works by slipping btwn base pairs of dbl str. dna. it fluoresces at 500nm, and will detect down to 50ng of dna.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] ethidium bromide [Answer]
|
a powerful mutagen. a fluorescent dye. fluoresces intensely at 560nm when excited by UV light (260-360nm), permitting detection of as little as 20ng dna.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] competent cells [Answer]
|
cells that have been treated so they are temporarily able to take up DNA.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Method for making competent cells [Answer]
|
cold CaCl2 treatment of cells that are dividing rapidly, followed by heat shock.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] What cell phase is transformation most successful in? [Answer]
|
early-mid log phase.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] How is it believed that the CaCl2 allow dna molecules to cross through the plasma membrane? [Answer]
|
it is proposed that dna cross through adhesion zones where there are pores formed by the fusion of the inner and outer mbrn. adhesion zns only form in growing cells. Ca2+ shields - charge on dna and pm, allowing close contact,this may be favored by low T
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] how is it believed the heat shock helps with dna crossing through the PM? [Answer]
|
The heat shock could briefly destabilize the membrane so that somehow the dna is allowed to get across.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] JM109-DE3 [Answer]
|
a strain of E.coli carrying the gene for T7 RNA polymerase.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] T7RNA polymerase [Answer]
|
binds to the T7 promoter region in the pRSET-B plasmin and synthesizes mRNA coding for the regions marked in the pRSET-B plasmid map.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Why is JM109-DE3 preferred over BL21-DE3? [Answer]
|
JM109-DE3 grow slower, giving FP's time to mature to their full fluorescence.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] 2 controls for transformation [Answer]
|
1. competent cells w/out plasmid: positive control 2. competent cells w/out plasmid on LB + ampicillin: negative control
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] What does the positive control tell us? (comp cells w/out plasmid) [Answer]
|
If nothing grows, then something may be wrong with competent cells.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] What does the negative control tell us? (comp cells w/out plasmid + LB and ampicillin) [Answer]
|
tests to be sure ampicillin is still good (good for 2-3 months). Should see nothing.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] 3 pieces of evidence when determining the identity of the fluorescent protein. [Answer]
|
1. Color of bacterial colony expressing the gene. 2. absorption spectrum 3. observing MW on SDS-PAGE gel.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] His-Tag was added to which end of the FP? [Answer]
|
the N-terminal end.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] NTA stands for [Answer]
|
nitrilotriacetic acid
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] NTA [Answer]
|
is a spacer molecule that nickel is chelated to.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] the lysis and column wash buffers have a low concentration of free imidazole, what does this low concentration do? [Answer]
|
competes out low affinity binding to the resin of a single or two adjacent histidines in a protein.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] What role does the high concentration of imidazole in the elution buffer perform? [Answer]
|
It is high enough to compete successfully for the nickel and displace the his-tagged proteins.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] A molecule will only absorb light when... [Answer]
|
it contains a dipole that can be made to oscillate at the same frequency as the incident light. light sensing molecules in our retinas contain these dipoles.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] following the absorption of photons, the 3 principle processes by which the excitation energy is dissipated or converted are: [Answer]
|
heat, chemical reactions and fluorescence.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Why didn't we use reducing agents when we ran our SDS-PAGE gels? [Answer]
|
to maintain fluorescence. prolonged exposure to SDS will also reduce fluorescence. This is why samples were saved in elution buffer not sample buffer.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] what stain did we use on our SDS-PAGE gels? [Answer]
|
Coumassie blue
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Where is the GFP fluorophore located in the AA sequence? [Answer]
|
Ser 65, Tyr 66, Gly 67
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] What issues had to be addressed in order to make FP's more useful in the laboratory? [Answer]
|
1. Brightness of fluorescence 2. Color, the more colors, the more flexibility when planning experiments 3. Folding temperature,needed to be able to fold properly at warmer temperatures, at which bio systems and prtns of interest fcn 4. work as fusion prot
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Where is the DsRed fluorophore located in the AA sequence? [Answer]
|
Gln 66, Tyr 67, Gly 68
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Why is wild type DsRed problematic when labeling proteins of interest? [Answer]
|
It is a tetramer, and it is difficult to label a protein of interest with an FP that is more than one polypeptide.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] What needed to be done in order to make DsRed useful? [Answer]
|
prevent the formation of a tetramer, and to obtain active fluorescence in the monomer form.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
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0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] How many aa substitutions went into mRFP1? [Answer]
|
33, but, from here, further substitutions produced variations in the color of the fluorescence.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Why was some of the sequence of GFP added to DsRed derivatives? [Answer]
|
after all the work to produce mRFP1, it did not work well as a fusion protein.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] fusion protein [Answer]
|
the protein produced by combining coding regions of the genes of two different proteins in order to link them together as a continuous polypeptide.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Can things around the fluorophore also affect fluorescence? [Answer]
|
Yes, by changing the electrostatic environment around the rings.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] To get wild type FP's to fold correctly, what aa substitution was made? [Answer]
|
Phe64Leu
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Blue shift [Answer]
|
ECFP Tyr66Trp: Green to blue (shorter wave length emission max) Means emission to shorter wavelength
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Red shift [Answer]
|
EYFP Ser65Gly shifts to a longer wavelength emission
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] Using a FP to label a protein of interest [Answer]
|
Use a mammalian expression vector and insert a fusion protein (inframe)
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] An example of FRET-Using a FP: [Answer]
|
Calcium binding protein fusion protein to detect Ca2+ influx. FRET needs 2 fluorophores.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] FRET stands for [Answer]
|
Fluorescence Resonance Energy Transfer
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
0136fa84_hcards_about_UCSD_BIBC3_Exam_3__Answer
|
[Question] bio luminescence in Jelly fish [Answer]
|
an enzyme called acquorin catalyzes a luminescent chemical rxn. the light given off is "blue light". If GFP is close to acquorin, RET occurs.
|
[] |
Flashcards about UCSD BIBC3 Exam 3
|
Answer
|
http://www.studystack.com/flashcard-415050
|
22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] Special Amount Column [Answer]
|
a journal amount column headed with an account title
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] General Amount Column [Answer]
|
journal amount column that is not headed with an account title
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] entry [Answer]
|
information for each transaction recorded in a journal
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] Double entry accounting [Answer]
|
recording of debit and credit parts of transaction
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] Source document [Answer]
|
business paper from which ino is obtained for a journal entry
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] invoice [Answer]
|
form describing the goods or services sol, the quanity, and the price
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] sales invoice [Answer]
|
invoice used as a source document for recording a sale on account
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] check [Answer]
|
business form ordering a bank to pay cash from a bank account
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] memorandumn [Answer]
|
form on which brief message is written describing a transaction
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] receipt [Answer]
|
business form giving written acknowledgement for cash received
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
fa5c9328_rds_about_Accounting_Chapter_4__Answer
|
[Question] proving cash [Answer]
|
determining that the amount of cash agrees with the accounting records
|
[] |
Flashcards about Accounting Chapter 4
|
Answer
|
http://www.studystack.com/flashcard-722094
|
22/1438043062635.98_20150728002422-00227-ip-10-236-191-2_726965531_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] foreign policy [Answer]
|
the strategies and goals that guide a nation's relations with other countries
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] national security [Answer]
|
protection of a nation's borders and territories against invasion of control by foreign powers
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] isolationism [Answer]
|
the avoidance of involvement in world affairs
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] Monroe Doctrine [Answer]
|
a foreign policy establisehd in 1823 to isolate Eurpore from the United States
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] containment [Answer]
|
foreign policy that stated that the U.S. would respond to any action by the Soviets; they didn't want communism to spread
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] detene [Answer]
|
a relaxation of tensions between nations
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] Commander-in-Chief [Answer]
|
President's job to control the army
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] Head of State [Answer]
|
President is the symbol of the country
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] ambassador [Answer]
|
an official of the government who represents the nation in dipolmatic matters
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] treaty [Answer]
|
a formal agreement between the governments of two or more countries
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] Secretary of State [Answer]
|
head of cabinet; gives president info
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] Secretary of Defense [Answer]
|
head of cabinet; gives president info
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] National Security Advisor [Answer]
|
director of the National Security Council Staff
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] Central Intelligence Agency [Answer]
|
gathers info about other countrie to give to the president
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] executive agreements [Answer]
|
agreement made between president and head of state
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] most-favored-nation [Answer]
|
reduces tariff rates on all exports to the nation; can be overturnedby 2/3 congressional vote
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] bipartisan [Answer]
|
consisting of both major political parties
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] embassy [Answer]
|
an ambassador's official residence/office in a foreign country
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] consulate [Answer]
|
office that promotes American business/safegaurds its travelers its travelers in a foriegn country
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] mutual defense alliances [Answer]
|
an agreement between nations to supprt each other in an attack
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] regional security pacts [Answer]
|
mutual defense treaty among nations of a region
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] North Atlantic Treaty Organization (NATO) [Answer]
|
US and European countries are allies against attacks
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] multilateral treaties [Answer]
|
international agreement signed by several nations
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] bilateral treaties [Answer]
|
agreement between two nations
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] collective security [Answer]
|
nations agree to take action with each other if attacked
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] Agency for Internation Developmentt (AID) [Answer]
|
government agency to provide aid for international development
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] sanctions [Answer]
|
withholding economic aid to influence a foreign governments activities
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
7b2731be_rds_about_Foreign_Policy_Vocab__Answer
|
[Question] internationalism [Answer]
|
involvment in world affairs
|
[] |
Flashcards about Foreign Policy Vocab
|
Answer
|
http://www.studystack.com/flashcard-35061
|
22/1438042990609.0_20150728002310-00121-ip-10-236-191-2_740625307_3.json
|
e429f9ac_rds_about_Ms_Joyner___fam__law__Answer
|
[Question] elder abuse [Answer]
|
Mistreatment, in any form, of older people.
|
[] |
Flashcards about Ms Joyner - fam. law
|
Answer
|
http://www.studystack.com/flashcard-812032
|
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
|
e429f9ac_rds_about_Ms_Joyner___fam__law__Answer
|
[Question] neglect [Answer]
|
Not taking proper care of someone in your care; this could be a child, an elderly person, or even a spouse.
|
[] |
Flashcards about Ms Joyner - fam. law
|
Answer
|
http://www.studystack.com/flashcard-812032
|
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
|
e429f9ac_rds_about_Ms_Joyner___fam__law__Answer
|
[Question] child abuse [Answer]
|
mistreatment of a child of a sexual, physical, or emotional nature; battering; beating; hurting.
|
[] |
Flashcards about Ms Joyner - fam. law
|
Answer
|
http://www.studystack.com/flashcard-812032
|
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
|
e429f9ac_rds_about_Ms_Joyner___fam__law__Answer
|
[Question] sexual abuse [Answer]
|
this occurs when someone uses another person for sexual reasons; it is usually an adult who uses a child this way, but not always
|
[] |
Flashcards about Ms Joyner - fam. law
|
Answer
|
http://www.studystack.com/flashcard-812032
|
22/1438042990177.43_20150728002310-00187-ip-10-236-191-2_724038599_3.json
|
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