PMCID
string
Title
string
Sentences
string
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Following the isolation of total RNA, the quality and concentration of the obtained RNA were assessed using a NanoDrop.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Then, the mRNA expression of p53, Bax, and Bcl-2 genes was analyzed using the Light Cycler 96 instrument system (Roche, USA).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The primer sequences were listed in Table 1.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The 2 method was used to determine the fold change of gene expression (27).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The primer sequences for qRT-PCR analysis.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Data were reported as mean ± standard deviation and analyzed using GraphPad Prism 8.2.1 (San Diego, CA, USA).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
A one-way ANOVA followed by a Tukey post-hoc test was used to show statistically significant differences between the groups.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
P < 0.05 was considered a significant difference.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
All experiments were examined in triplicate.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
SH-SY5Y cell viability was assessed using the MTT assay after 24 h treatment with various concentrations of α-LA, D-gal, and Met.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The results showed that treatment with concentrations higher than 1500 μM of α-LA significantly reduced the SH-SY5Y cell viability (Fig. 1A).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
However, concentrations of 31.25, 62.5, and 125 μM of α-LA did not show any significant effect on cell viability, so these non-toxic concentrations were used for further experiments.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The results also showed that Met up to 20 mM had no notable impact on the viability of SH-SY5Y cells (Fig. 1B).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Therefore, the 5 mM concentration of Met was used for further experiments (2).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
On the other hand, D-gal significantly reduced cell viability, and the half-maximal inhibitory concentration (IC50) value for D-gal was calculated to be 155 mM (Fig. 1C).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Therefore, a D-gal concentration of 155 mM was used for subsequent experiments.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Effect of (A) α-LA, (B) Met, and (C) D-gal on SEESY5Y cell viability after 24 h; (D) the protective effect of α-LA concentrations (31.25, 62.5, and 125 μM) against the D-gal-induced cytotoxicity in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
MTT assay was used for evaluating cell viability.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Untreated and D-gal + Met groups were assigned as negative control and positive control groups, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Met and D-gal were used at the concentrations of 5 and 155 mM, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
P < 0.001 indicates significant difference compared to untreated group; P < 0.01 and P < 0.001 versus D-gal group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
α-LA, α-lipoic acid; Met, metformin; D-gal, D-galctose.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The MTT assay was also carried out to evaluate the protective effect of α-LA against the cytotoxicity of D-gal in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The findings indicated that α-LA at concentrations of 31.25, 62.5, and 125 μM significantly reduced D-gal-induced cytotoxicity in SH-SY5Y cells (Fig. 1D).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The ONPG assay was conducted to assess the cellular senescence induced by D-gal in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The findings revealed a notable increase in beta-galactosidase levels in the D-gal group in comparison to the untreated cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
However, α-LA and Met significantly decreased the level of beta-galactosidase and thus D-gal-caused senescence compared to the D-gal group (Fig. 2).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Effect of α-LA (62.5 and 125 μM) on D-gal-induced senescence in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Senescence-associated β-galactosidase activity was examined using the ONPG.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Untreated and D-gal + Met groups were assigned as negative control and positive control groups, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Met and D-gal were used at the concentrations of 5 and 155 mM, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
P < 0.001 indicates significant difference compared to untreated group; P < 0.001 versus D-gal group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
α-LA, α-lipoic acid; Met, metformin; D-gal, D-galctose; ONPG, o-nitrophenyl beta-D-galactopyranoside.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The ROS assay findings indicated a notable increase in ROS levels in SH-SY5Y cells following treatment with D-gal compared to untreated cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
On the other hand, treatment with α-LA and Met significantly reduced the ROS levels induced by D-gal (Fig. 3).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Effect of the α-LA (62.5 and 125 μM) on ROS production in SH-SY5Y cells after 24 h. Intracellular ROS levels were determined by using the fluorescent ROS indicator dye, 2ʹ,7ʹ-dichloro dihydrofluorescein diacetate.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Untreated cells and TBHP were used as the negative and positive controls, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Met and D-gal were used at the concentrations of 5 and 155 mM, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
P < 0.001 indicates significant difference compared to untreated group; P < 0.001 versus D-gal group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
TBHP, Tert-butyl hydroperoxide; α-LA, α-lipoic acid; Met, metformin; D-gal, D-galactose; ROS, reactive oxygen species.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
According to the findings, the level of MDA in the D-gal group exhibited a significant increase in comparison to the untreated cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
However, the MDA level in the α-LA + D-gal and Met + D-gal groups was significantly reduced in comparison to the D-gal group (Fig. 4A).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Total thiol levels in the D-gal group showed significant reduction compared to the untreated group, while total thiol levels significantly increased in the α-LA + D-gal and Met + D-gal groups compared to the D-gal group (Fig. 4B).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
In addition, the results indicated that SOD activity in the D-gal group was significantly reduced compared tothe untreatedgroup, while treatment with α-LA and Met modulated this effect and significantly enhanced the SOD activity in comparison to the D-gal group (Fig. 4C).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Effect of α-LA(62.5 and 125 μM) on (A) MDA, (B) total thiol, and (C) SOD levels in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Untreated and D-gal + Met groups were assigned as negative control and positive control groups, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Met and D-gal were used at the concentrations of 5 and 155 mM, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
P < 0.001 indicates significant difference compared to untreated group; P < 0.01 and P < 0.001 versus D-gal group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
α-LA, α-lipoic acid; Met, metformin; D-gal, D-galctose; MDA, malondialdehyde; SOD, superoxide dismutase.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The mRNA expression of apoptosis-related genes was investigated by quantitative real-time PCR (qRT-PCR) in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The findings revealed that treatment with 155 mM of D-gal significantly increased the expression of Bax and p53 genes, while decreasing the Bcl-2 gene expression compared to the untreated group (Fig. 5).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
However, treatment with α-LA and Met reversed these effects and significantly reduced the expression of pro-apoptotic Bax and p53 genes, while increasing the expression of the anti-apoptotic Bcl-2 gene compared to the D-gal group (Fig. 5).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Effect of α-LA on mRNA expression of (A) Bax, (B) Bcl-2, and (C) p53 in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
SH-SY5Y cells were exposed to 62.5 and 125 μM of α-LA, 5 mM of Met, and 155 mM of D-gal.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
mRNA expression of apoptosis-related genes was assessed using quantitative real-time PCR.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Untreated and D-gal + Met groups were assigned as negative control and positive control groups, respectively.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
P < 0.001 indicates significant difference compared to untreated group; P < 0.05 and P < 0.01 versus D-gal group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
α-LA, α-lipoic acid; Met, metformin; D-gal, D-galctose.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
In many tissues, free radical damage and mitochondrial dysfunction lead to biological aging.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The effects of oxidative stress on mitochondria have been demonstrated in neurons under pathological conditions (28).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
a-LA is one of the most promising antioxidants that has recently been noted, and it reacts with ROS (29).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
By scavenging the harmful molecules, α-LA helps prevent oxidative damage to cellular components.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Additionally, it can stimulate the expression and activity of cellular antioxidant enzymes, such as SOD and glutathione peroxidase, which further enhance the cell's ability to combat oxidative stress (30).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
D-gal has been extensively used in studies to simulate aging by producing oxidative stress (456).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
D-gal mimics the aging process in cells by increasing ROS when galactitol accumulates in cells (6).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
In addition, D-gal increases MDA levels, decreases the antioxidant capacity, and activity of antioxidant enzymes such as SOD, which causes oxidative stress and premature aging (6).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Therefore, the current study aimed to evaluate the protective effects of α-LA on D-gal-induced cellular senescence in SH-SY5Y neuronal cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The results revealed that α-LA reduced the cytotoxicity and senescence caused by D-gal.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
α-LA also effectively reduced the ROS generation compared to the D-gal group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Treatment with α-LA significantly modulated the levels of MDA, total thiol, and SOD activity, which were altered by D-gal.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
In addition, treatment with α-LA decreased the expression of Bax and p53 genes, while increasing the expression of the Bcl-2 gene.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
A common marker of senescence in cells and animals is the increased activity of SA-β-Gal.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
SA-β-gal is an enzymatic activity of β-galactosidase that can be detected at pH 6.0 in cultured cells experiencing replicated or induced senescence, while it is not present in actively dividing cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
SA-β-gal activity has been found in the organs of elderly humans as well as animals, revealing that senescent cells accumulate in tissue with age, and that cellular senescence is a characteristic of organismal aging (31).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
A typical measurement of SA-β-gal activity utilizes the ONPG color assay (21).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
In the present study, ONPG results showed an increase in β-galactosidase level in the group treated with D-gal alone, while β-galactosidase decreased in the groups treated with α-LA and Met.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
In this regard, Baeeri et al. investigated the anti-senescence effects of α-LA on rat embryonic fibroblast cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
They reported that β-galactosidase and oxidative stress markers were significantly decreased following α-LA treatment (32).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Oxidative stress arises from an imbalance between the generation of ROS and the capacity of cells to neutralize effectively or repair the resulting damage.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
ROS are highly reactive molecules that can cause harm to cell structures such as proteins, lipids, and DNA.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
This damage can lead to disruptions in cellular functions and signaling pathways, ultimately contributing to various diseases and aging processes.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
ROS can affect lipid membranes by causing damage, resulting in lipid peroxidation.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Additionally, ROS can induce oxidation of amino acid residues present in proteins, altering their structure and functionality.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
These changes can have a significant impact on enzymatic activities, signaling pathways, and the overall balance within the cell.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Furthermore, ROS can directly target DNA, causing various modifications including base oxidation, DNA strand breaks, and DNA crosslinking.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Failure to repair the DNA damage can result in mutations, genomic instability, and ultimately cell death (33).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Hence, antioxidants are employed as a therapeutic strategy to address abnormalities linked to oxidative stress (34).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Zhao et al. demonstrated that the R form of α-LA is effective in reducing 6-hydroxydopamine (6-OHDA)-induced toxicity in SH-SY5Y cells (35).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The current findings indicated that treatment with α-LA at concentrations of 62.5 and 125 μM effectively reduced D-gal-induced ROS levels in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Consistent with the findings, it has been indicated that α-LA can effectively reduce ROS levels in the PC12 cell line induced by 6-OHDA (36).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Furthermore, a study showed that α-LA effectively reduced ROS levels induced by cadmium toxicity in HepG2 cells through the Nrf2/ARE signaling pathway (37).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Najafi et al found that α-LA significantly reduced the toxicity caused by high glucose via increasing the activity of catalase and SOD antioxidant enzymes in PC12 cells (38).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
In the previous study conducted on the protective effects of α-LA in quinolinic acid-treated cells, α-LA at non-toxic concentrations was shown to protect against quinolinic acid-induced toxicity.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
In addition, pre-treatment with α-LA reduced ROS levels and the number of sub-G1 cells in quinolinic acid-treated SH-SY5Y cells (39).
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The current study evaluated the effect of a-LA on the SOD activity, MDA level, and total thiol content in SH-SY5Y cells.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
The results indicated that the MDA levels in SH-SY5Y cells were considerably higher in the D-gal group compared to the untreated group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
However, the MDA level was significantly lower in the α-LA + D-gal and Met + D-gal groups.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Thiol levels and SOD enzyme activity were remarkably decreased in the D-gal group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
On the other hand, total thiol levels and SOD activity in the α-LA + D-gal and Met + D-gal groups were significantly higher than in the D-gal group.
PMC12614211
The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line
Additionally, α-LA is protective against oxidative stress induced by arsenic in rat liver mitochondria, and treatment with α-LA reduced the levels of MDA and ROS and improved catalase activity, as well as improving mitochondrial membrane damage (40).