PMCID string | Title string | Sentences string |
|---|---|---|
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | Next, we applied two different analyses to measure cell death. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | We further analyzed the FACS data, particularly cell populations at sub-G1 phase, in which cells undergo cell death. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | This cell population contained both floating cells in culture media and cells attached to the culture plate. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | We found additive effects on cell death indices in combined treatment groups with KCC-07 and DNA damaging reagents in both cell lines (Fig. 3B). |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | Additionally, dead cells were measured in real-time by live cell imaging using IncuCyte. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | However, the dead cell index was not significantly different between cell lines with DNA damage induction and combined treatment (Fig. 3C), most likely due to measurement of only the attached cell population on the plate using IncuCyte. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | Of note, SH-SY5Y cell lines showed aggregation of cells upon KCC-07 treatment, which was also detected in combined treatment groups, indicating that cell response to KCC-07 might vary depending on cell lines. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | Moreover, we measured proliferation indices in continuous exposure to KCC-07 and/or DNA damaging reagents. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | There was further proliferation reduction in U-87MG and SH-SY5Y cell lines with combined treatment of KCC-07 and DNA damaging reagents compared to individual treatments (Fig. 4A), suggesting that additive effects on growth suppression of tumor cells could be achieved by combined treatment with KCC-07 and DNA damage induction. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | Collectively, these data demonstrate the expandable application of KCC-07 to other neural tumors and its potential to enhance the efficacy of existing treatments. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | The combination approach with KCC-07 suggested here could enable dose reduction of DNA damaging agents, substantially mitigating their associated toxicity while preserving or even enhancing therapeutic efficacy for brain tumors. |
PMC12426421 | KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells | In addition, further studies are warranted to identify the specific mediators that bridge MBD2 inhibition and p53 pathway activation in U-87MG and SH-SY5Y cell lines, which could provide valuable insights for optimizing KCC-07 based therapeutic strategies in neural tumors. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Background and Objectives: Neuroblastoma is the most common extracranial solid tumor in children, often presenting challenges in treatment due to its clinical and genetic heterogeneity. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | This study investigated the anticancer potential of Pelargonium sidoides root extract on the human neuroblastoma cell line (SH-SY5Y). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Using XTT assays, ELISA-based oxidative stress markers, and RT-PCR analysis of apoptotic genes, the study explored the extract’s effects on cell proliferation, oxidative stress, and apoptosis. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Materials and Methods: For the cell culture, SH-SY5Y human neuroblastoma cells were thawed, cultured, and maintained under appropriate conditions for experiments. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The dose- and time-dependent activity of Pelorgonium sidoides extract on SH-SY5Y neuroblastoma cells was investigated by XTT assay. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The change in the oxidative stress marker 8-Hydroxy-2′-deoxyguanosine (8-OhDG) level was determined by ELISA for the doses applied to the control group root extract at a concentration of 25 μg/mL. Total antioxidant status (TAS) and total oxidant status (TOS) were measured from the cells in the study group with the help of a commercial kit. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The oxidative stress index (OSI) was calculated by dividing the TAS by the TOS and multiplying by 100. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | In order to evaluate the expression levels of apoptosis-related Bax, Bcl-2, Caspase-3, Caspase-8, and Caspase-9 genes at the mRNA level in control and dose group cells, RNA isolation was performed from the SH-SY5Y control and dose group cells (IC50 value). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Results: It is observed that the P. sidoides substance inhibits proliferation in cells at 24 h (p < 0.05). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | As the dose increases, cell proliferation decreases (p < 0.05). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The IC50 value was calculated to be 113.83 μg/mL at 24 h. The concentration of 8-OhDG increased in neuroblastoma cells as a result of P. sidoides extract treatment (p < 0.05). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | TOS levels increased in neuroblastoma cells treated with P. sidoides extract (p < 0.01). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | OSI levels increased in cells treated with P. sidoides extract (p < 0.001). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | BAX and Caspase-8 expression increased are statistically significant in the P. sidoides dose group (p < 0.05). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Conclusions: P. sidoides extract induces apoptosis in neuroblastoma cells through oxidative stress and mitochondrial- and death receptor-mediated pathways. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | This study highlights the potential of P. sidoides as a complementary therapeutic agent for neuroblastoma, warranting further in vivo and clinical investigations to assess its safety and efficacy. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Childhood cancers stand out as a serious public health problem in the medical field. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Every year, thousands of children are diagnosed with cancer worldwide, and these diseases are responsible for a significant portion of childhood deaths . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Early diagnosis and treatment of these cancers are of great importance for both the course of the disease and the quality of life of patients. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Cancers seen in childhood generally originate from cells with high proliferative potential, and in addition to genetic factors, environmental effects also play a role in the emergence of these diseases . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Among childhood cancers, neuroblastoma holds a particularly critical position. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | It is the most common extracranial solid tumor diagnosed in children and accounts for approximately 15% of all pediatric cancer-related deaths . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Originating from primitive neural crest cells in the embryonic period, this tumor is the third most common type of cancer in childhood, with a rate of 7% . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Neuroblastoma, which shows great clinical and genetic heterogeneity, presents challenges that limit the effectiveness of current treatment methods . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The pathogenesis of neuroblastoma involves a complex interplay of genetic, epigenetic, and environmental factors. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Approximately 90% of neuroblastoma patients exhibit chromosomal changes, including MYCN amplification, ALK mutations, and 1p or 11q deletions, which are linked to disease progression and poor outcomes . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | These alterations underscore the need for targeted therapies that can address these genetic aberrations. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Current treatment strategies for neuroblastoma typically include surgery, chemotherapy, radiotherapy, and immunotherapy. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | However, these approaches often come with significant toxicity, particularly in young patients, and are limited in their efficacy for high-risk cases. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | As such, there is an urgent need for novel therapeutic strategies that can improve survival while minimizing side effects. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | In recent years, intensive studies have been conducted on the role of plant-based bioactive molecules in cancer treatment . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | It has been shown that these molecules have the potential to suppress tumor proliferation, activate apoptosis mechanisms, and regulate oxidative stress levels . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Pelargonium sidoides (P. sidoides) root extract has been used in traditional treatment methods for a long time and has been found to be effective in modern medicine, especially in the treatment of upper respiratory tract infections . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | However, recent studies also focus on the potential anticancer effects of P. sidoides and show that this plant may affect various biological pathways associated with cancer . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The phenolic compounds of P. sidoides attract attention with their potential to regulate PI3K-Akt and Ras/MAPK pathways, which are cancer-related signaling pathways . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The aim of this study was to investigate the effects of P. sidoides root extract on the SH-SY5Y human neuroblastoma cell line. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The effects of the extract on cell proliferation, oxidative stress levels and apoptosis mechanisms were examined with various experimental methods, and the findings are presented in a way that will contribute to new treatment approaches for childhood cancers. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | This study was supported by the Ordu University scientific research project (BAP project number: A-2308). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The study was conducted between 6 June 2023, and 29 May 2024. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | For the cell culture, SH-SY5Y human neuroblastoma cells were thawed, cultured, and maintained under appropriate conditions for experiments. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Cell proliferation was achieved using DMEM-F12 culture medium containing 10% fetal bovine serum, 2 mM L-glutamine, and 1% penicillin–streptomycin in a 95% humidity and 5% CO2 oven at 37 °C. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Cells were multiplied under appropriate conditions and made ready for experiments. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | In order to renew our stocks, they were frozen in new cryos with DMSO and stored at minus 80 °C. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The dose- and time-dependent activity of Pelorgonium sidoides extract on SH-SY5Y neuroblastoma cells was investigated by XTT assay. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Briefly, cells plated in 96-well plates were treated with the synthesized substance at appropriate dose ranges specified in the literature for 24, 48, and 72 h, following an initial incubation period of 24 h. After incubation, XTT solution was added to the 96-well plates, and after 4 h, absorbance values (OD) were measured using an ELISA reader at a wavelength of 450 nm with a reference range of 630 nm. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The percentage of cell viability was calculated by dividing the optical density value measured in each well by the control optical density value and multiplying by one hundred. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Then, non-toxic doses were determined, and dose selection was made for further experiments. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The inhibitory concentration (IC50) value was calculated via the (https://www.aatbio.com/tools/ic50-calculator, accessed on 20 December 2023). % |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Cell viability = [(measured optical density value)/(control optical density value)] × 100. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The change in the oxidative stress marker 8-Hydroxy-2′-deoxyguanosine level was determined by ELISA for the doses applied to the control group root extract at a concentration of 25 μg/mL. A human 8-Hydroxy-deoxyguanosine ELISA kit (Cat No: EA0048Hu; Bioassay Technology Laboratory, Shanghai, China) was used. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The application procedure was as follows: First, the standard concentration provided with the kit was prepared by serial dilution. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Standard concentrations were adjusted to be 800, 400, 200, 100, and 50 ng/mL from the main stock of 1600 ng/mL. Then, 20 mL of wash solution provided as 30X was diluted to 1X with distilled water. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Kit components were brought to room temperature, and the study began. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Standards were loaded into 96-well ELISA plates as 50 μL. No antibody was added to the standards. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Samples were loaded as 40 μL and then 10 μL of anti-8-OHDG antibody was added. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Then, 50 μL of streptavidin–HRP was added to both standards and samples. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | This addition was not made for the blank well. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | After all these added components, incubation was applied for 60 min at 37 °C in the dark. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | At the end of the incubation, washing was performed 5 times for all wells with wash solution. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Then, substrate solution A and 50 μL of substrate solution B were pipetted. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Then, incubation was performed for 10 min at 37 °C in the dark. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | After the incubation was completed, 50 μL of stop solution was added and readings were performed using a microplate reader (Epoch 2) at 450 nm within 10 min. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | A similar protocol was applied for all other ELISA tests. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The principle of total oxidant level measurement is based on the conversion of the ferrous ion chelator complex of the oxidants in the sample to ferric ion, which reacts with the chromogen in an acidic environment and causes an increase in absorbance. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The increase in absorbance observed spectrophotometrically is directly proportional to the oxidant molecules in the sample . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The principle of total antioxidant level measurement is based on the principle that all antioxidants in the sample convert the blue–green ABTS radical into colorless reduced ABTS . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | In this study, a control and dose group were created for SH-SY5Y neuroblastoma cells in order to investigate the effect of P. sidoides root extract. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Total oxidant and total antioxidant levels were measured from the cells in the study group with the help of the commercial kit provided (TAS and TOS, Rel Assay, Gaziantep, Turkey. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | OSI was calculated by dividing the TAS by the TOS and multiplying by 100. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The experiments were repeated three times to ensure the reliability of the experiments . |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | In order to evaluate the expression levels of apoptosis-related Bax, Bcl-2, Caspase-3, Caspase-8, and Caspase-9 genes at the mRNA level in control and dose group cells, RNA isolation was performed from the SH-SY5Y control and dose group cells (IC50 value). |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Neuroblastoma cells were plated in 6-well cell culture plates at a density of 1 × 10 to 2 × 10 cells per well and incubated for 24 h to achieve appropriate confluency for further experiments. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Then, control and dose groups were adjusted. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | At the end of incubation, the medium in the wells was removed and the cells were washed with 3 mL cold PBS. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | After removing the PBS, 500 μL Trizol was added to the wells to lift the cells. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The homogenate was transferred to 1.7 mL Eppendorf tubes and incubated for 10 min at room temperature. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Then, 100 μL of chloroform was added to each Eppendorf tube and vortexed, and incubated again at room temperature for 15 min. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Then, the tubes were centrifuged at 15,000× g for 15 min at 4 °C, and the colorless upper phase containing RNA was collected and transferred into separate Eppendorf tubes. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | A total of 250 μL of isopropanol was added to the collected upper phase, pipetted, and left at room temperature for 10 min. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | After the tubes were centrifuged at 15,000× g for 10 min at +40 °C, the supernatant was carefully discarded, and 70% ethanol was added to the pellet and centrifuged at 12,000× g for 10 min at +40 °C. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The supernatant then was discarded again and the pellet was air dried for a short time. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | Finally, the pellet was dissolved with 40 μL of RNase–DNase-free water. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | The quantity and quality of the obtained RNAs were determined using Nanodrop. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | A260/A280 and A260/A230 ratios were evaluated to determine the phenol, protein, and genomic DNA contamination that may occur during isolation. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | After UV measurements, RNA samples with 2 ± 0.1 for A260/A280 and 2.0–2.4 for A260/A230 were used in the analyses. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | For mRNA expression analysis, cDNA synthesis was performed from isolated RNAs using the A.B.T.™ cDNA Synthesis Kit with RNase Inhibitor (High-Capacity) synthesis kit (CatNo: C03-01-20, ABT, Ankara, Turkey) along with a random hexamer, dNTP, and reverse transcriptase enzyme (RT) followingg the manufacturer’s protocol. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | After the mixture was prepared, it was incubated at 25 °C for 10 minutes and then at 37 °C for 120 minutes for cDNA synthesis. |
PMC11679892 | Effects of Pelargonium Sidoides Extract on Apoptosis and Oxidative Stress in Human Neuroblastoma Cells | At the end of this period, the mixturewas kept at 85 °C for 5 min to inhibit the enzyme. |
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