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1d0f566bbf3fa3360c9413fa41a8d66b22360deed9a0cf45c1cf1fec6012052b
Python
189,635
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from __future__ import annotations from collections.abc import ( Callable, Collection, Generator, Hashable, Iterable, Sequence, ) from functools import wraps from itertools import zip_longest from sys import getsizeof from typing import ( TYPE_CHECKING, Any, Literal, NoReturn, ...
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Python
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""" Provide the groupby split-apply-combine paradigm. Define the GroupBy class providing the base-class of operations. The SeriesGroupBy and DataFrameGroupBy sub-class (defined in pandas.core.groupby.generic) expose these user-facing objects to provide specific functionality. """ from __future__ import annotations f...
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Python
193,209
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from __future__ import annotations from collections.abc import ( Callable, Collection, Generator, Hashable, Iterable, Sequence, ) from functools import wraps from itertools import zip_longest from sys import getsizeof from typing import ( TYPE_CHECKING, Any, Literal, NoReturn, ...
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Python
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"""test to_datetime""" import calendar from collections import deque from datetime import ( UTC, date, datetime, timedelta, timezone, ) from decimal import Decimal import locale import re import zoneinfo from dateutil.parser import parse import numpy as np import pytest from pandas._libs import t...
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Python
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"""test to_datetime""" import calendar from collections import deque from datetime import ( UTC, date, datetime, timedelta, timezone, ) from decimal import Decimal import locale import re import zoneinfo from dateutil.parser import parse import numpy as np import pytest from pandas._libs import t...
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Python
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# -*- coding: utf-8 -*- # Form implementation generated from reading ui file 'GUI.ui' # # Created by: PyQt5 UI code generator 5.11.3 # # WARNING! All changes made in this file will be lost! from PyQt5 import QtCore, QtGui, QtWidgets class Ui_MainWindow(object): def setupUi(self, MainWindow): MainWindow.s...
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Python
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# pylint: skip-file """File that contains the Christiansen Bosonic Operator data generated via functions in vibrant.""" # fmt: off # Reference generated from vibrant # mol = H2S # coords =[[0.0, -1.0, -1.0], [0.0, 1.0, -1.0], [0.0, 0.0, 0.0]] # localize = True # nbos = 4 # quad_order = 9 # cubic = True from pennylan...
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Python
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import textwrap import time as pytime from collections import defaultdict import subprocess import scipy.interpolate import scipy.linalg import scipy.signal import scipy.stats from sklearn.metrics import accuracy_score, f1_score from .backend import * from .data import build_CDR_impulse_data, build_CDR_response_data,...
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Python
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from typing import Iterable, List, Literal, Mapping, Optional, Sequence, Tuple, Union import numpy as np import pandas as pd import tqdm from numba import jit _Peakcalling_Method = Optional[Literal["CCcaller", "MACCs", "Blockify"]] _reference = Optional[Literal["hg38", "mm10", "sacCer3"]] _PeakTestMethod = Optional[L...
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Python
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""" DataFrame --------- An efficient 2D container for potentially mixed-type time series or other labeled data series. Similar to its R counterpart, data.frame, except providing automatic data alignment and a host of useful data manipulation methods having to do with the labeling information """ from __future__ impor...
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Python
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#! /usr/bin/env python ############################################################################## ## DendroPy Phylogenetic Computing Library. ## ## Copyright 2010-2015 Jeet Sukumaran and Mark T. Holder. ## All rights reserved. ## ## See "LICENSE.rst" for terms and conditions of usage. ## ## If you use this wo...
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Python
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from __future__ import annotations from collections import abc from datetime import ( datetime, tzinfo, ) import functools from itertools import zip_longest import operator from typing import ( TYPE_CHECKING, Any, ClassVar, Literal, NoReturn, Self, SupportsIndex, cast, final...
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Python
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""" DataFrame --------- An efficient 2D container for potentially mixed-type time series or other labeled data series. Similar to its R counterpart, data.frame, except providing automatic data alignment and a host of useful data manipulation methods having to do with the labeling information """ from __future__ impor...
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Python
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#name name2 NM_000299 PKP1 NM_001276351 C1orf141 NM_001005337 PKP1 NM_001276352 C1orf141 NR_075077 C1orf141 NM_001042681 RERE NM_012102 RERE NR_038261 HIVEP3 NM_001042682 RERE NM_001281956 CSMD2 NM_052896 CSMD2 NM_001130058 SLC44A5 NM_001350214 TTLL7 NM_001320287 SLC44A5 NM_024686 TTLL7 NM_001350215 TTLL7 NM_001320285 ...
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Python
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from __future__ import annotations from collections import abc from datetime import ( datetime, tzinfo, ) import functools from itertools import zip_longest import operator from typing import ( TYPE_CHECKING, Any, ClassVar, Literal, NoReturn, Self, SupportsIndex, cast, final...
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Python
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""" High level interface to PyTables for reading and writing pandas data structures to disk """ from __future__ import annotations from contextlib import suppress import copy from datetime import ( date, tzinfo, ) import itertools import os import re from textwrap import dedent from typing import ( TYPE_C...
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Python
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# pyright: reportPropertyTypeMismatch=false from __future__ import annotations import collections from copy import deepcopy import datetime as dt from functools import partial from json import loads import operator import pickle import re import sys from typing import ( TYPE_CHECKING, Any, ClassVar, Co...
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# pyright: reportPropertyTypeMismatch=false from __future__ import annotations import collections from copy import deepcopy import datetime as dt from functools import partial from json import loads import operator import pickle import re import sys from typing import ( TYPE_CHECKING, Any, ClassVar, Co...
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""" High level interface to PyTables for reading and writing pandas data structures to disk """ from __future__ import annotations from contextlib import suppress import copy from datetime import ( date, tzinfo, ) import itertools import os import re from textwrap import dedent from typing import ( TYPE_C...
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Python
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""" Data structure for 1-dimensional cross-sectional and time series data """ from __future__ import annotations from collections.abc import ( Callable, Hashable, Iterable, Mapping, Sequence, ) import functools import operator import sys from typing import ( IO, TYPE_CHECKING, Any, ...
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Python
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""" Data structure for 1-dimensional cross-sectional and time series data """ from __future__ import annotations from collections.abc import ( Callable, Hashable, Iterable, Mapping, Sequence, ) import functools import operator import sys from typing import ( IO, TYPE_CHECKING, Any, ...
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from __future__ import division import sys import numpy as np import torch import scipy import copy from itertools import combinations from sklearn.manifold import MDS import nn_analyses as nna import nn_models as nm import task_train_test as tk import nn_plot_functions as npf def get_trai...
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--- title: "Dorgau_lu pseudobulk" format: html: self-contained: true --- ```{r global_options, include=FALSE} knitr::opts_chunk$set(echo=FALSE, warning=FALSE, message=FALSE, fig.width = 10, fig.height = 10) ``` Can the new data be integrated successfully with previously published data or do they appear to be...
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--- title: "FlowSOM_PDG_BrM" author: "Benoit Duc" format: html editor: visual --- ## FlowSOM analysis of PDGfp and breast-BrM tumors ```{r setup} library(data.table) library(janitor) library(tidyverse) library(flowCore) library(FlowSOM) library(pheatmap) ``` ```{r function-definitions} ###########...
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--- format: html: self-contained: true --- # Lu et al, 2020 ### Single-Cell Analysis of Human Retina Identifies Evolutionarily Conserved and Species-Specific Mechanisms Controlling Development {-} ```{r global_options, include=FALSE} knitr::opts_chunk$set(echo=FALSE, warning=FALSE, message=FALSE, fig.width ...
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--- title: "HIFI Spatial Analysis - Neighborhood Analysis" format: html editor: visual --- ### Neighborhood Analysis This script takes single cell HIFI data from QuPath after cell type annotation and analyzes cellular neighborhoods. Interaction analysis is included to perform statisitical tests of cellular co...
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--- title-block-banner: true title: "Creation of point patterns using the spatstat package" date: today date-format: full author: - name: "Daniel Manrique-Castano" orcid: 0000-0002-1912-1764 affiliation: Univerisity Laval (Laboratory of neurovascular interactions) keywords: - GFAP analysis -...
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--- title: "HIFI Spatial Analysis - Proximity Networks" format: html editor: visual --- ### **Proximity Network Analysis** This notebook creates graph-based networks of cellular proximity from HIFI digital pathology data. The code generates a matrix of unidirectional cell type connections (currently all-to...
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--- title: "Dissecting glial scar formation by spatial point pattern and topological data analysis" subtitle: "Supplementary data analysis notebook" format: html: code-fold: true embed-resources: true toc: true toc-location: left theme: spacelab knitr: opts_chunk: warning: fals...
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ftrans <- function(a){ return(log((1+a)/(1-a))) }
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R
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library(testthat) library(UCell) test_check("UCell")
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R
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soft <- function(x,d){ return(sign(x)*pmax(0, abs(x)-d)) }
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#' @useDynLib fibrationSymmetries #' @importFrom Rcpp sourceCpp NULL
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l2n <- function(vec){ a <- sqrt(sum(vec^2)) if(a==0) a <- .05 return(a) }
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### export_deps.R ### write.csv(installed.packages(), "./tools/requirements_r.csv")
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install.packages("psych") library(psych) r.test(n=91, r12=.29, r13=.07,r23=.55)
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zscore <- function(x, na.rm=TRUE){ (x-mean(x, na.rm=na.rm))/sd(x, na.rm=na.rm) }
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#' @name snpsOfInterest #' @title snpsOfInterest #' @description Example SNPs of interest from simulated \code{gwasResults} data. #' @docType data NULL
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#' @name gwasResults #' @title Simulated GWAS results #' @description Simulated GWAS results as obtained from \code{plink --assoc}. #' @docType data NULL
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dlpfc <- list(spatial = readRDS('data-raw/LIBD_spatial_3639cells_2cols.rds'), count_exp = readRDS('data-raw/LIBD_mrna_count_18011genes_3639cells.rds')) usethis::use_data(dlpfc)
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workflowr::wflow_publish( c( "analysis/figure_2d-THP7.Rmd", "analysis/figure_2e-Pr5P7.Rmd" ), verbose = TRUE ) # workflowr::wflow_publish("analysis/eda.Rmd", all = TRUE, update = TRUE, republish = TRUE, verbose = TRUE)
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library(DiffBind) K27ac_B<-dba(sampleSheet = "K27ac_B.csv",dir=system.file("extra", package="DiffBind")) K27ac_B_consensus<-dba.peakset(K27ac_B,bRetrieve = TRUE) write.csv(K27ac_B_consensus,file="C:/Users/Jerry Liu/Documents/K27ac_B_consensus.csv")
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library(tidyverse) library(stringr) library(R.matlab) library(cowplot) library(expm) library(mgcv) library(RColorBrewer) library(lm.beta) library(wesanderson) library(rjson) library(reshape2) library(reticulate) library(car) library(viridis) library(ggrepel)
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# Side effects of upon package loading and unloading .onLoad <- function(libname, pkgname) { # Initialize global options ecOptions() invisible() } .onUnload <- function(libpath) { # Reverse side effects options(ecOptions = NULL) invisible() }
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#SR_TAU_CELL .libPaths("/data/user/jfisher7/.conda/envs/SR_TAU_CELL/lib/R/library") #make sure the environment is clean rm(list=ls()) info<- sessionInfo() print(info) # Listing packages packages<- installed.packages()[,c(1,3)] print("Listing packages") print(packages)
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library(ggplot2) library(stringr) library(R.matlab) library(cowplot) library(expm) library(mgcv) library(RColorBrewer) library(lm.beta) library(wesanderson) library(rjson) library(stringr) library(reshape2) library(reticulate) library(car) library(viridis) library(gsubfn) library(ggrepel)
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#' STew S3 object #' #' @param count_exp count_exp data #' @param spatial spatial data #' #' @return S3 Object #' @export STew_Obj = function(count_exp = NULL, spatial = NULL){ stew = list() stew$count_exp <- count_exp stew$spatial <- spatial class(stew) <- 'STew_Object' return (stew) }
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#' List all tips descendant from a node #' #' @param tree an object of class 'phylo'. #' @param node number of interest. #' @return vector of tip numbers. #' @export getTips<-function(tree,node){ getDescendants(tree,node)[which(getDescendants(tree,node)<(length(tree$tip.label)+1))] }
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## this function returns the name of the sheet from the filepath getRegion = function(f) { x = unlist(strsplit(f, split = "/", fixed = TRUE)) x = x[3] x = unlist(strsplit(x, "_", fixed = TRUE)) x = x[-c(1:5)] x = x[1:(length(x) - 1)] # remove last segment x = paste(x, collapse = "_") return(x) }
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#' List all nodes descendant from a node #' #' @param tree an object of class 'phylo'. #' @param node number of interest. #' @return vector of node numbers. #' @export getNodes<-function(tree,node){ c(getDescendants(tree,node)[which(getDescendants(tree,node)>(length(tree$tip.label)+1))],node) }
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# install the required R packages for the demos install.packages("Rcpp",dependencies=TRUE) install.packages("Matrix",dependencies=TRUE) install.packages("pracma",dependencies=TRUE) install.packages("RcppAnnoy",dependencies=TRUE) install.packages("RSpectra",dependencies=TRUE) install.packages("igraph",dependencies=TRUE)...
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#install.packages("irr") library("irr") #install.packages("readr") library(readr) data1 <- read_csv("pubmed.csv") data2 <- read_csv("scopus.csv") combined_data <- rbind(data1, data2) # binarize it combined_data <- combined_data + 1 combined_data <- sign(combined_data) kappam.fleiss(combined_data, detail = TRUE) agr...
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# This file is part of the standard setup for testthat. # It is recommended that you do not modify it. # # Where should you do additional test configuration? # Learn more about the roles of various files in: # * https://r-pkgs.org/tests.html # * https://testthat.r-lib.org/reference/test_package.html#special-files libr...
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#' List all edges descendant from a node #' #' @param tree an object of class 'phylo'. #' @param node number of interest. #' @return vector of edge numbers. #' @export getEdges<-function(tree,node){ Desc<-getDescendants(tree,node) Edges<-c() for(i in 1:length(Desc)){Edges<-c(Edges,which(tree$edge[,2...
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library(randomForest) load('/Users/jianinglu1/Documents/GitHub/deltaVinaXGB_develop/Feature/RF20.rda') args <- commandArgs(trailingOnly = TRUE) infn = args[1] outfn = args[2] df = read.table(infn, header=T, stringsAsFactors = F, sep=',') feats = df[3:22] pred = predict(rffit, newdata = feats) + df$vina output = data.fr...
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.onLoad <- function(libname, pkgname) { desc <- read.dcf(file.path(system.file(package = pkgname, lib.loc = libname), "DESCRIPTION")) cat("Package:", desc[, "Package"], "\n") cat("Description:", desc[, "Description"], "\n") cat("Version:", desc[, "Version"], "\n") cat("Release Date:", desc[, "Date"], "\n") ...
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### DATA d <- read.table("GSE93374_Merged_all_020816_DGE.txt") d <- d[,order(colnames(d))] ### ANNOTATIONS ann <- read.table("GSE93374_cell_metadata.txt", sep = "\t", header = T) ann <- ann[order(ann[,1]), ] rownames(ann) <- ann[,1] ann <- ann[,2:ncol(ann)] colnames(ann)[9] <- "cell_type1" ### SINGLECELLEXPERIMENT so...
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--- title: "221221_rstudio_cancer_dr_info" author: "Jennifer Fisher" date: '2022-12-21' output: html_document: default --- GitHub Repo: Transfer_Leanring_R03 Docker: rstudio_cancer_dr Directory of operations: /home/rstudio (Docker) ```{r setup, include=FALSE} knitr::opts_chunk$...
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### bioinfo_installer.R ### install.packages("BiocManager", repos="http://cran.ism.ac.jp/") BiocManager::install("clusterProfiler") BiocManager::install("AnnotationDbi") BiocManager::install("org.Hs.eg.db") BiocManager::install("org.Mm.eg.db") BiocManager::install("org.Rn.eg.db") BiocManager::install("GO.db") install....
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convert_evetech_names = function(x) { x = gsub("SDF-1α\\+β", "SDF-1ab", x) x = gsub("FLT-3L", "Flt-3l", x) x = gsub("GROα", "GRO", x) x = gsub("IFN-α2", "IFNa2", x) x = gsub("γ", "g", x) x = gsub("IL-1α", "IL-1a", x) x = gsub("β", "b", x) x = gsub("MIP-1α", "MIP-1a", x) x = gsub("δ", "d", x) x = gsu...
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### DATA d <- read.csv("data.csv") ### ANNOTATIONS genes <- unlist(lapply(strsplit(as.character(d[,1]), "_"), "[[", 2)) d <- d[!duplicated(genes), ] rownames(d) <- genes[!duplicated(genes)] d <- d[,2:ncol(d)] # metadata ann <- data.frame(cell_type1 = unlist(lapply(strsplit(colnames(d), "__"), "[[", 2))) rownames(ann) ...
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.onAttach <- function(...){ packageStartupMessage() packageStartupMessage("For example usage please run: vignette('qqman')") packageStartupMessage() packageStartupMessage("Citation appreciated but not required:") packageStartupMessage("Turner, (2018). qqman: an R package for visualizing GWAS results...
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# Set directory to source file loaction setwd(paste0(figureDirectory, "Figure7-survival" ) ) library(ggplot2) library(cowplot) load("plotListSurvival.RData") png(filename = "Figrue7.png", height = 1000, width = 1000, res = 90) plot_grid(plotList[[9]],plotList[[2]],plotList[[3]],plotList[[5]],plotList[[11]],plotLi...
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R
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install.packages("psych") library(psych) # week 8 r.test(n = 24, r12 = 0.62, r13 = 0.48, r23=.97, twotailed = FALSE) # TSI vs MAIP r.test(n = 23, r12 = 0.62, r13 = 0.21, r23=.55, twotailed = FALSE) # TSI vs mathres r.test(n = 24, r12 = 0.62, r13 = 0.06, r23=.27, twotailed = FALSE) # TSI vs numops # week 4 r.test(...
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R
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#' Extract variant type (snp/indel) #' #' This function takes in a CollapsedVCF object and returns a vector: variant type (snp/indel) #' #' @param VCF_DATA CollapsedVCF object (as extracted by VariantAnnotation::readVcf()) #' @return non null GT vector snp_indel <- function( VCF_DATA ){ cat( " -> extracting varian...
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R
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res1 <- read.delim("AXXC-WT_deseq2_LFC.txt",row.names = "gene_symbol") head(res1) library(EnhancedVolcano) EnhancedVolcano(res1, lab = rownames(res1), x = 'log2FoldChange', y = 'padj', xlim = c(-3, 3), ylim = c(-0.5,80), ...
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R
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### This code creates fig. S6a dev.off() rm(list=ls()) library(ggplot2) library(tidyr) library(gridExtra) library(dplyr) Data <- read.table("Chr12_binning_summary_10kb", header = F) Data <- Data[-6111,] Data$V2 <- as.integer(Data$V2) pdf("TE_density.pdf", width=5, height=5, useDingbats=FALSE) ggplot(Data, aes(x=V2, ...
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R
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--- title: "221221_rstudio_tf_dr_v3_singularity" author: "Jennifer Fisher" date: "12/21/2022" output: html_document: default --- GitHub Repo: Transfer_Learning_R03 Docker: rstudio_tf_dr_v3 Directory of operations: /data/project/lasseigne_lab/JLF_scratch/Transfer_Learning_R03 (singula...
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R
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BinarySearch <- function(argu,sumabs){ if (length(sumabs) == 0) { sumabs <- 0 } if(l2n(argu)==0 || sum(abs(argu/l2n(argu)))<= sumabs) return(0) lam1 <- 0 lam2 <- max(abs(argu))-1e-5 iter <- 1 while(iter < 150){ su <- soft(argu,(lam1+lam2)/2) if(sum(abs(su/l2n(su)))<sumabs){ lam2 <- (lam1...
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R
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### DATA d <- read.table("nature13173-s4.txt") d <- t(d) genes <- d[,1][5:nrow(d)] # remove genes and bulk samples d <- d[,2:(ncol(d) - 2)] exprs_data <- as.data.frame(matrix(as.numeric(d[5:nrow(d),]), ncol = ncol(d))) rownames(exprs_data) <- genes colnames(exprs_data) <- d[1,] ### ANNOTATIONS ann <- data.frame(cell_t...
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R
510
19
library(Seurat) library(DropletUtils) library(argparse) library(dplyr) parser <- ArgumentParser("Parameters for 10x matrix") parser$add_argument("--hto_h5", help = "Path to HTO H5 ") parser$add_argument("--hto_mtx_path", help = "Path to save HTO mtx. ") args <- parser$parse_args() hto_h5 <- Read10X_h5(args$hto_h5) ...
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R
511
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### DATA d <- read.table("GSE87544_Merged_17samples_14437cells_count.txt", header = TRUE) rownames(d) <- d[,1] d <- d[,2:ncol(d)] d <- d[,order(colnames(d))] ### ANNOTATIONS ann <- read.csv("GSE87544_1443737Cells.SVM.cluster.identity.renamed.csv") rownames(ann) <- ann[,2] colnames(ann)[3] <- "cell_type1" ann <- ann[,3...
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R
515
19
library(ggplot2) rm(list = ls()) options(stringsAsFactors = F) QC_K27 = read.csv('RNA_QC.csv') colnames(QC_K27) pdf("RNA_deduplication.rate.pdf",height = 5,width = 8) ggplot(QC_K27,aes(x=condition,y=deduplication.rate,fill=condition)) + geom_violin(trim = TRUE) + geom_boxplot(width=0.2,fill="white") + scale_co...
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R
515
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library(rmarkdown) library(dplyr) allData <- "/data/rachel/Linda_Lako/Spatial/spaceranger_outs_2021_102" tissue <- "AD3" projDir <- paste0("/data/rachel/Linda_Lako/Spatial/", tissue) outputDir <- file.path(projDir, "/rObjects/") ## find markers sampleNames <- paste0("AD3_D", c(10,20,35,60,90,150,210)) for (sampleNa...
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R
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test_that("bamEdges() working", { test.bam <- test_path("fixtures", "sample.bam") test.edges <- test_path("fixtures", "sample.bamEdges.rds") expect_true( all(bamEdges(test.bam) == readRDS(test.edges)) ) }) test_that("countEdges() working", { test.bam <- test_path("fixtures", "sample.bam") ...
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R
525
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# Function that takes in a vector of p-values and outputs a corresponding number of stars p_star <- function(x){ if(sum(is.na(x)) != 0){ warning("Vector contains NA values") } star <- factor() star <- sapply(x, function(i) { if(i <= 10^(-4)){"****" ...
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R
534
16
### DATA data1 <- read.table("ExprMat1.txt", header=T) ann1 <- read.table("Ann_part1.txt", header=T) data2 <- read.table("ExprMat2.txt", header=T) ann2 <- read.table("Ann_part2.txt", header=T) DATA <- cbind(data1, data2) ANN <- rbind(t(ann1), t(ann2)) ### ANNOTATIONS rownames(ANN) <- NULL colnames(ANN) <- c("Source", ...
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R
549
21
#DeLong test library(pROC) y<-c() for(i in 1:300){ y<-c(y,0,1) } df<-read.delim('C:/Users/oonar/Downloads/unet_binClass_predictions.txt', header=FALSE) dim(df) x<-df[,1] df<-read.delim('C:/Users/oonar/Downloads/inception_binClass_predictions.txt', header=FALSE) z<-df[,1] roc...
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R
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23
make.kNNG <- function(DM, k=as.integer( 2*log(nrow(DM)) ), symm =FALSE, weight=FALSE ) {#begin function N <- nrow(DM) ADM <- matrix(0, N, N) #Search kNN point if(weight==TRUE){ for (i in 1:N) { nid <- order(DM[i,]) ADM[ i, nid[2:(k+1)] ] <- DM[ i, nid[2:(k+1)] ] } }else...
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R
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#' Combine colData and in_tissue column of SpatialData from SpatialExperiment into a data.frame #' #' @param spe SpatialExperiment object with per spot QC metrics in colData and #' in_tissue column present in spatialData #' #' @return data.frame containing both colData and in_tissue from spatialData of original spe s...
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R
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### _go_pipeline.R ### suppressPackageStartupMessages({ library(clusterProfiler) library(optparse) source("/home/jovyan/tools/_go.R") }) optslist <- list( make_option( c("-t", "--tempdir"), type="character", default="/home/jovyan/out", help="temporary directory to save ...
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R
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### _gprofiler_pipeline.R ### suppressPackageStartupMessages({ library(optparse) source("/home/jovyan/tools/_gprofiler.R") }) optslist <- list( make_option( c("-t", "--tempdir"), type="character", default="/home/jovyan/out", help="temporary directory to save intermediate fi...
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R
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M3_RNA_count<-read.csv("E:/R files/M3_RNA_count.csv",row.names = 1) M3_RNA_count<-as.matrix(M3_RNA_count) M3_RNA_count_coldata<-read.csv("E:/R files/M3_RNA_count_coldata.csv",row.names = 1) M3_RNA_count_coldata<-as.matrix(M3_RNA_count_coldata) library("DESeq2") dds <- DESeqDataSetFromMatrix(countData = M3_RNA_coun...
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R
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HG38_FASTA_FILE <- '/home/enrico/columbia/FASTA/hg38.fa' CHR_NAMES <- c("chr1","chr2","chr3","chr4","chr5","chr6","chr7","chr8","chr9","chr10","chr11","chr12","chr13","chr14","chr15","chr16","chr17","chr18","chr19","chr20","chr21","chr22","chrX","chrY","chrM") genomestrings <- Biostrings::readDNAStringSet(HG38_FASTA_...
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R
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#' joint_embedding() #' #' Extracting spatial-informed canonical loading #' #' @param obj stCCA S3 Object #' #' @export joint_embedding <- function(obj) { stcc <- obj$optimal_cca meta_cell <- stcc$v rownames(meta_cell) <- stcc$znames meta_cell <- data.frame(meta_cell) for ( col in 1:ncol(meta_cell)){ co...
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R
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#' Calculating row mean for each gene using normalized gene expression matrix #' #' @param obj stCCA object #' #' @export #' #'@import tibble #'Matrix #'matrixStats var_genes <- function(obj){ environment(FindVariableGenesSeurat) <- asNamespace("Seurat", base.OK = TRUE) exp <- Matrix(obj$count_exp, sparse = TRUE)...
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R
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#' return a vector with null GT removed #' #' This function takes in a GT vector object and returns it without null GTs #' #' @param GT_VECTOR vector object with GT (as extracted by VariantAnnotation::readVcf()) #' @param GT_NULL (optional) vector passing GT values to consider null (default: c( '0/0', './.', '0|0', '.|...
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R
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#' Barplot of canonical correlation for each pair of canonical variate #' #' @param cc Output of CCA() function. Result of Sparse CCA. #' @param t Title #' @param tsub Subtitle #' #' @export #' #' @import ggplot2 #' ggpubr plot_corr <- function(cc, t, tsub=NULL) { cca_cor <- data.frame(cc$cors) cca_cor$CC <- paste...
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R
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### _get_go.R ### suppressPackageStartupMessages({ library(clusterProfiler) library(optparse) source("/home/jovyan/tools/_go.R") }) optslist <- list( make_option( c("-t", "--tempdir"), type="character", default="/home/jovyan/out", help="temporary directory to save inter...
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R
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### DATA d <- read.table("data.txt") # select 5 patients d <- d[,grepl("MGH26_", colnames(d)) | grepl("MGH264_", colnames(d)) | grepl("MGH28_", colnames(d)) | grepl("MGH29_", colnames(d)) | grepl("MGH30_", colnames(d)) | grepl("MGH31_", colnames(d))] ### ANNOTATIONS patients <- unlis...
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R
623
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library(Signac) library(Seurat) library(GenomeInfoDb) library(EnsDb.Hsapiens.v86) library(ggplot2) library(dplyr) library(patchwork) library(rtracklayer) library(cowplot) set.seed(1234) sampleInfo <- readRDS("rObjects/SampleInfo.rds") sObj_predicted <- readRDS("rObjects/sObj_predictions.rds") for (i in 1:length(sO...
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R
623
23
.libPaths("/home/u23/haozhang1/miniconda2/envs/r_env/lib/R/library") args = commandArgs(TRUE) options(stringsAsFactors = FALSE) library(tidyverse) library(Matrix) ################## # load binary matrix bmat = read_tsv(args[1]) #bmat = bmat[, -c(1:3)] #bmat = bmat %>% # column_to_rownames("annot") #rownames(bmat) = ...
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R
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library(stats) ChooseLambda1Lambda2 <- function(y,chrom=NULL){ # using as of 8/25/09 if(is.null(chrom)) chrom <- rep(1, length(y)) y.lo <- lowess(y, f=min(50/length(y),.2))$y y.lo <- soft(y.lo, quantile(abs(y.lo), .45)) s1=sum(abs(y.lo)) lambdas=exp(seq(log(10^(-4)), .2, len=50)) out <- matrix(NA, nrow=leng...
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R
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# Generated by using Rcpp::compileAttributes() -> do not edit by hand # Generator token: 10BE3573-1514-4C36-9D1C-5A225CD40393 calculateVectors <- function(nodeColors, edges, directed, weighted, numberOfWeights, fromR = FALSE) { .Call('_fibrationSymmetries_calculateVectors', PACKAGE = 'fibrationSymmetries', nodeCol...
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R
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library(dplyr) library(Seurat) library("ggplot2") library(monocle3) library(patchwork) library(SeuratWrappers) library(harmony) library(ggplotify) library(rmarkdown) subsets <- readRDS("rObjects/subsets_list.rds") for (i in 1:9){ outName <- names(subsets)[i] seuratObj_subset <- SetIdent(seuratObj_subset, value ...
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R
657
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### install_deps.R ### deps <- read.csv("./tools/requirements_r.csv", row.names=1) "%notin%" <- Negate("%in%") current_pkg <- installed.packages() for(package in rownames(deps)) { if (package %notin% rownames(current_pkg)) { remotes::install_version(package, version = deps[package,"Version"], repos="http:...
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R
663
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### DATA d <- read.table("data.txt", header = T) d <- d[!duplicated(d$ID),] rownames(d) <- d$ID d <- d[,2:ncol(d)] ### ANNOTATIONS ann <- read.table("biase_cell_types.txt", stringsAsFactors = F) ### SINGLECELLEXPERIMENT source("../utils/create_sce.R") sceset <- create_sce_from_normcounts(d, ann) # convert ensembl ids...
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R
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CHR_NAMES <- c("chr1","chr2","chr3","chr4","chr5","chr6","chr7","chr8","chr9","chr10","chr11","chr12","chr13","chr14","chr15","chr16","chr17","chr18","chr19","chr20","chr21","chr22","chrX","chrY") CODING_LENGTH <- c( 8345447,5878924,4836344,3417120,3959062,4251743,3840011,2910726,3182390,3240638,4701713,4544590,1644627...
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R
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sce <- import(sampleDirs = c("/data1/zj_mut/")) sce.raw <- import(sampleDirs = c("/data1/zj_mut/"), dataType = "raw") scemut <- decontX(sce, background=sce.raw) umap <- reducedDim(scemut, "decontX_UMAP") plotDimReduceCluster(x = scemut$decontX_clusters, dim1 = umap[, 1], dim2 = umap[, 2]) plotDecon...
4436cbe4022860fab0df8d2f70311d4da4bb501eb0434f700f09bf475051f24d
R
669
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library(Spaniel) library(scran) library(scater) library(batchelor) library(dplyr) library(Seurat) library(SCFunctionsV3) library(cowplot) library(RColorBrewer) library(SCFunctionsV3) library(harmony) allData <- "/data/rachel/Linda_Lako/Spatial/spaceranger_outs_2021_102" tissue <- "fetal_retina" projDir <- paste0("/d...