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9006020 | Secretion of nuclease across the outer membrane of Serratia marcescens and its energy requirements. | Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its exp... |
9006012 | Detection and comparison of specific hemin binding by Porphyromonas gingivalis and Prevotella intermedia. | A radioligand assay was designed to detect and compare specific hemin binding by the periodontal anaerobic black-pigmenting bacteria (BPB) Porphyromonas gingivalis and Prevotella intermedia. The assay included physiological concentrations of the hemin-binding protein rabbit serum albumin (RSA) to prevent self-aggregati... |
9001247 | Role of hydrophobic amino acid clusters in the transactivation activity of the human glucocorticoid receptor. | We have performed a mutagenesis analysis of the 58-amino-acid tau1-core peptide, which represents the core transactivation activity of the tau1 transactivation domain from the glucocorticoid receptor. Mutants with altered activity were identified by phenotypic screening in the yeast Saccharomyces cerevisiae. Most mutan... |
9001242 | Constitutive activation of Epstein-Barr virus (EBV) nuclear antigen 1 gene transcription by IRF1 and IRF2 during restricted EBV latency. | The Epstein-Barr virus (EBV) EBNA1 gene promoter active in the type I program of restricted viral latency was recently identified and shown to reside in the viral BamHI Q fragment. This promoter, Qp, is active in a wide variety of cell lines and has an architecture reminiscent of eukaryotic housekeeping gene promoters ... |
8995696 | The sequence element of the internal ribosome entry site and a 25-kilodalton cellular protein contribute to efficient internal initiation of translation of hepatitis C virus RNA. | Translation of hepatitis C virus (HCV) RNA is initiated by internal entry of ribosomes into the 5' noncoding region (NCR). This process depends on genomic elements within the 5' NCR called the internal ribosome entry site (IRES) and may involve host factors. The alpha-branch structure (nucleotides 47 to 67) of the HCV ... |
8995680 | The barley stripe mosaic virus 58-kilodalton beta(b) protein is a multifunctional RNA binding protein. | The barley stripe mosaic virus (BSMV) beta(b) gene product is the major viral nonstructural protein synthesized during early stages of the infection cycle and is required for systemic movement of the virus. To examine the biochemical properties of beta(b), a histidine tag was engineered at the amino terminus and the pr... |
8995652 | Assembly of herpes simplex virus capsids using the human cytomegalovirus scaffold protein: critical role of the C terminus. | An essential step in assembly of herpes simplex virus (HSV) type 1 capsids involves interaction of the major capsid protein (VP5) with the C terminus of the scaffolding protein (encoded by the UL26.5 gene). The final 12 residues of the HSV scaffolding protein contains an A-X-X-F-V/A-X-Q-M-M-X-X-R motif which is conserv... |
8995649 | Overlapping epitopes in human immunodeficiency virus type 1 gp120 presented by HLA A, B, and C molecules: effects of viral variation on cytotoxic T-lymphocyte recognition. | Human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTL) are thought to exert immunologic selection pressure in infected persons, yet few data regarding the effects of this constraint on viral sequence variation in vivo, particularly in the highly variable Env protein, are available. In this study, CD8... |
8995608 | An amino-terminal domain of the Sendai virus nucleocapsid protein is required for template function in viral RNA synthesis. | The nucleocapsid protein (NP) of Sendai virus encapsidates the genome RNA, forming a helical nucleocapsid which is the template for RNA synthesis by the viral RNA polymerase. The NP protein is thought to have both structural and functional roles, since it is an essential component of the NP0-P (P, phosphoprotein), NP-N... |
10374481 | [Choice of time and mode of operation in severe acute pancreatitis]. | We report the treatment of 75 patients of severe acute pancreatitis. Operations were performed on patients with pancreatic infection and organ failure according to the principle of "individualization". Operation was also indicated for the patients aged 60 who had had no improvement after a short period of non-operative... |
28862565 | Enzymatic Synthesis of Mannosyl-cyclodextrin by α-Mannosidase from Jack Bean. | Mannosylated derivatives of cyclodextrins (CDs), mannosyl-α, β, and γCD were synthesized from a mixture of mannose and α, β, and γCD by the reverse action of α-mannosidase from jack bean, respectively. Their structures were analyzed by FAB-MS and <sup>13</sup>C-NMR spectroscopies, and they were identified as 6-O-α-d-ma... |
27396736 | Isolation of Taka-amylase A Peptides Required for Substrate Binding. | An α-amylase from Aspergillus oryzae, Taka-amylase A (TAA), was cleaved into peptide fragments by an acid protease. Inactivation of TAA was greatly retarded by the addition of α-cyclodextrin or Ca(2+). TAA peptide fragments were separated into two groups having no and high affinity to the substrate, soluble starch. Thi... |
22358518 | Development of a bioartificial liver employing xenogeneic hepatocytes. | Liver failure is a major cause of mortality. A bioartificial liver (BAL) employing isolated hepatocytes can potentially provide temporary support for liver failure patients. We have developed a bioartificial liver by entrapping hepatocytes in collagen loaded in the luminal side of a hollow fiber bioreactor. In the firs... |
18475810 | Antitumor Activity of the Carboplatin-alpha-Cyclodextrin Adduct. | Antitumor activity of the adduct between Carboplatin and alpha-cyclodextrin has been tested against Sarcoma 180 (S(180)) and Ehrlich ascites carcinoma (EAC) murine tumors. The preliminary toxicity has also been evaluated by histological examinations of the treated animals. The results show the adduct has less antitumor... |
18475797 | Synthesis, Characterisation and Antifungal Activities of Some New Copper(II) Complexes of Isomeric 3,5,7,7,10,12,14,14-Octamethyl-1,4,8,11-Tetraazacyclotetradecanes. | Three isomeric Me(8)[14]anes, L(A), L(B) and L(C), undergo complexation with copper(II) salts to form a series of [CuLX(n)(H(2)O)(x)]X(y).(H(2)O)(z) complexes where L = L(A), L(B) and L(C); X = Cl, Br, NO(3); n, x, y and z may have values of 0, 1 or 2. The complexes have been characterised on the basis of analytical, s... |
18475787 | Antiviral metal complexes. | The initial events (virus adsorption and fusion with the cells) in the replicative cycle of human immunodeficiency virus (HIV) can serve as targets for the antiviral action of metal-binding compounds such as polyanionic compounds (polysulfates, polysulfonates, polycarboxylates, polyoxometalates, and sulfonated or carbo... |
18475786 | Properties of paramagnetic metals in MRI. | The use of different metals in Magnetic Resonance Imaging will be briefly described.This overview will be more a qualitative description than a full paper on this topic, in other words, this paper contributes to explaining how and why some metal ions have been chosen for MRI.Paramagnetic compounds are currently used in... |
18475781 | Workshop on: Chemistry of Metals in Medicine - The Industrial Perspective. | The Workshop was attended by 61 participants from 20 countries. Most of the speakers were industrialists and the Chairpersons and Discussion Leaders were academics.The area "Chemistry of Metals in Medicine" has the potential for producing innovative, high quality, and original research.This is a new and emerging area o... |
18472839 | Eicosanoid and cytokine levels in plasma of patients during mesenteric infarction. | Multible organ failure (MOF) induced by mesenteric infarction is associated with a high mortality rate. This study reports eicosanoid and cytokine levels in the blood of three atherosclerotic patients who ultimately died from MOF induced by mesenteric infarction. High plasma levels of 6- keto-prostaglandin (PG) F(1alph... |
9028882 | The Effect of Sodium Dodecyl Sulfate and M-alpha-Cyclodextrin on the Solubility and Partitioning of Xylenes in Aqueous Solutions | The solubility and the partitioning of p- and o-xylene in aqueous solutions containing M-alpha-CD, a partially methylated alpha-cyclodextrin, and the anionic surfactant sodium dodecyl sulfate (SDS) were studied by extraction experiments to evaluate the applicability of liquid membrane permeation for the separation of x... |
11671410 | Total Synthesis of Dehydrodidemnin B. Use of Uronium and Phosphonium Salt Coupling Reagents in Peptide Synthesis in Solution. | New total syntheses of didemnin A and of dehydrodidemnin B are described. The latter didemnin has the highest antiproliferative activity of all members of this family of macrocyclic depsipeptides. It was produced on coupling the side chain Pyr-Pro-OH to didemnin A, which was itself synthesized by two novel routes. One ... |
11671372 | Complexes of Macrocycles with gamma-Cyclodextrin As Deduced from NMR Diffusion Measurements. | The complexes of 12-crown-4 (12C4), cyclen (12N4), and 1,4,7,10-tetrathiacyclododecane (12S4) with gamma-cyclodextrin (gamma-CD) were studied by NMR diffusion measurements in the absence and in the presence of inorganic and organic salts. The 12-crown-4:gamma-CD system was also used to evaluate the effect of the nature... |
11671361 | Recognition of Bipyridinium-Based Derivatives by Hydroquinone- and/or Dioxynaphthalene-Based Macrocyclic Polyethers: From Inclusion Complexes to the Self-Assembly of [2]Catenanes. | A range of pi-electron-rich macrocyclic polyethers incorporating dioxybenzene (hydroquinone) and/or dioxynaphthalene units have been synthesized in good yields by simple two-step procedures. These macrocycles are able to bind bipyridinium-based guests as a result of a series of cooperative noncovalent bonding interacti... |
9460561 | Molecular automata assembly: principles and simulation of bacterial membrane construction. | The motivation to understand the basic rules and principles governing molecular self-assembly may be relevant to explain in the context of molecular biology the self-organization and biological functions exhibited within cells. This paper presents a molecular automata model to simulate molecular self-assembly introduci... |
9460056 | Adsorption of haematoporphyrins on the planar bilayer membrane. | Adsorption of haematoporphyrin derivatives with different hydrophobicities of peripheral groups on a planar bilayer lipid membrane (BLM) was studied in the dark and upon illumination by the visible light. Haematoporphyrin molecules were shown to adsorb on the BLM as anions. The adsorption changed the boundary potential... |
9451948 | Molecular recognition in fungal mating. | Fungal cells need to recognize that they are genetically different in order to mate. Cells signal to each other by secreting small peptide pheromones which are detected by cell surface receptors. New transcriptional regulators are formed that alter the pattern of gene transcription. Basidiomycete fungi are unusual in h... |
9451786 | Dielectric measurements of lysozyme and tri-N-acetyl-D-glucosamine association at radio and microwave frequencies. | Time domain dielectric measurements were applied to the monitoring of molecular recognition by proteins. Lysozyme and tri-N-acetyl-D-glucosamine((NAG)3) were selected as a typical lock and key type recognition system. After association of (NAG)3, relaxation related to lysozyme itself was increased and depended on the p... |
9442897 | Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. | Eukaryotic cells evolved when their genetic information was packed into the cell nucleus. DNA replication and RNA biogenesis occur inside the nucleus while protein synthesis takes place in the cytoplasm. Bi-directional trafficking between these two compartments is mediated by a single supramolecular assembly, the nucle... |
9442881 | The two-component signaling pathway of bacterial chemotaxis: a molecular view of signal transduction by receptors, kinases, and adaptation enzymes. | The chemosensory pathway of bacterial chemotaxis has become a paradigm for the two-component superfamily of receptor-regulated phosphorylation pathways. This simple pathway illustrates many of the fundamental principles and unanswered questions in the field of signaling biology. A molecular description of pathway funct... |
9435099 | Synthesis and structural determination of stereoisomers of muscarinic ligands of the (3-propylthio-1,2,5-thiadiazol-4-yl)-1-azabicycloalkane type. | Methods for the synthesis of each of the four stereoisomers of 6-(3-propylthio-1,2,5-thiadiazol-4-yl)-1-azabicyclo[3.2.1]oc tane (10, 11, 12, and 13) and 3-(3-propylthio-1,2,5-thiadiazol-4-yl)-1-azabicyclo[2.2.1]he ptane (18, 19, 20, and 21), and the two stereoisomers of 3-(3-propylthio-1,2,5-thiadiazol-4-yl)-1-azabicy... |
9427006 | Structural and mechanistic consequences of polypeptide binding by GroEL. | The remarkable ability of the chaperonin GroEL to recognise a diverse range of non-native states of proteins constitutes one of the most fascinating molecular recognition events in protein chemistry. Recent structural studies have revealed a possible model for substrate binding by GroEL and a high-resolution image of t... |
9421888 | Impedance and shear wave resonance analysis of ligand-receptor interactions at functionalized surfaces and of cell monolayers. | The present paper scrutinizes the application of impedance spectroscopy and quartz-crystal microbalance (QCM) measurements in the analysis of composite layers of receptor containing lipid bilayers, and their interaction with external ligands or pore-forming peptides. The formation of supramolecular structures and their... |
9421884 | Role of conducting polymeric interfaces in promoting biological electron transfer. | In this paper, we explore the role that polymer conductivity and functionality play in determining the nature of molecular recognition at artificial polymer interfaces, as evidenced by electron transfer with the small redox protein, cytochrome c. The relationship is investigated electrochemically using cyclic voltammet... |
9422439 | Acute renal failure and outcome of continuous arteriovenous hemodialysis (CAVHD) and continuous hemofiltration (CAVH) in elderly patients following cardiovascular surgery. | A total of 111 elderly patients from the cardiac surgery intensive care unit (ICU) with acute renal failure (ARF) were studied during a period of 7 years (1988-1994). Forty-two patients being operated for coronary bypass (CBP) (31 M, 11 F), 26 patients for valve replacement (VR) (18 M, 8 F), 20 patients for a combined ... |
9413960 | Preliminary results of the use of intraperitoneal carbon-adsorbed mitomycin C in intra-abdominal malignancy. | Eleven patients suffering from intra-abdominal malignancy were treated with various doses of intraperitoneal mitomycin C adsorbed onto activated carbon particles. Seven of the patients underwent resection of their primary gastric tumour and all developed potentially life-threatening severe complications that proved to ... |
9366027 | Chirality of the gamma-lactones produced by Sporidiobolus salmonicolor grown in two different media. | Sporidiobolus salmonicolor is an aroma-producing yeast which gives a peach-like smell to the culture media. The enantiomeric ratios of the five gamma-lactones produced by this yeast cultivated in two different media were determined by multidimensional gas chromatography (MDGC) on a fused silica capillary column coupled... |
9366016 | Quartz crystal microbalances for quantitative biosensing and characterizing protein multilayers. | The use of quartz crystal microbalances (QCMs) for quantitative biosensing and characterization of protein multilayers is demonstrated in three case studies. Monolayers of QCM-based affinity biosensors were investigated first. Layers of a thiol-containing synthetic peptide constituting an epitope of the foot-and-mouse-... |
9366015 | A cell-based immunobiosensor with engineered molecular recognition--Part III: Engineering molecular recognition. | We have been studying the feasibility of exploiting the recognition and amplification abilities of living immune cells for the development of hybrid immunosensors. Our group has previously reported that cell metabolic activation responses, induced by calcium ionophore A23187, can be directly transduced using calorimetr... |
9384716 | Analysis of venlafaxine by capillary zone electrophoresis. | Capillary electrophoresis has been used for the separation of venlafaxine and two of its impurities deriving from the synthesis process. The electrophoretic experiments were performed using background electrolytes at different pHs in the 2.5-9.2 range in order to study the effective mobilities and resolution of the thr... |
9355703 | Glycosylation of hemoglobin and erythrocyte membrane proteins mediated changes in osmotic fragility of erythrocytes. | The increased levels of glycosylated hemoglobin and glycosylated erythrocyte membrane proteins in gestational diabetes could bring about alterations in osmotic fragility in erythrocytes of gestational diabetes during their first trimester. The glycosylated hemoglobin levels in erythrocytes were found to be significantl... |
9350510 | On the role of Müller glia cells in histogenesis: only retinal spheroids, but not tectal, telencephalic and cerebellar spheroids develop histotypical patterns. | The establishment of cell and fibre layers and the specification of different cell types are crucial processes during development of the central nervous system. Here we investigated the developmental architecture of radial glia cells in these processes using so-called spheroids that arise from dissociated chicken embry... |
8987783 | Activation of hippocampal adenosine A3 receptors produces a desensitization of A1 receptor-mediated responses in rat hippocampus. | The adenosine A3 receptor is expressed in brain, but the consequences of activation of this receptor on electrophysiological activity are unknown. We have characterized the actions of a selective adenosine A3 receptor agonist, 2-chloro-N6-(3-lodobenzyl)-adenosine-5'-N-methyluronamide (Cl-IB-MECA), and a selective A3 re... |
9332492 | Changes in the phosphorylation status of the 27 kDa heat shock protein (HSP27) associated with the modulation of growth and/or differentiation in MCF-7 cells. | We have used human mammary cells of the MCF-7 strain, which constitutively express high levels of the small heat shock protein HSP27 and we have compared the changes in the phosphorylation status of this protein together with changes in cell growth and/or morphology induced by the action of one of the following agents:... |
9332463 | Methyl-beta-cyclodextrin in HL-60 parental and multidrug-resistant cancer cell lines: effect on the cytotoxic activity and intracellular accumulation of doxorubicin. | The purpose of this work was to determine the role of methyl-beta-cyclodextrin (MEBCD) in combination with doxorubicin (DOX) on the cellular proliferation of a sensitive parental and a multidrug-resistant human cancer cell line (HL-60 S and HL-60 R) and to study the effect of MEBCD on DOX intracellular accumulation. Th... |
9309775 | The role of drugs in the control of parasitic nematode infections: must we do without? | Parasitic helminths (worms) cause serious infectious diseases in humans and domestic animals. Control of these infections relies mostly on chemotherapeutics (the anthelmintics), but resistance has developed against most of these broad-spectrum drugs in many parasite species. These resistant parasites are being used to ... |
9305550 | Non-random ionic-charge distribution responsible for the structural stability and molecular recognition of proteins. | The 'ionic-charge shuffling method' is presented to generate a complete set of electrostatic mutants for a natural protein where ionic charges on the molecular surface of the template protein are exhaustively interchanged with each other. Total Coulomb interaction energies are evaluated for all of the mutants by numeri... |
9277134 | Design of peptides, proteins, and peptidomimetics in chi space. | Peptide and protein biological activities depend on their three dimensionals structures in the free state and when interacting with their receptors/acceptors. The backbone conformations such as alpha-helix, beta-sheet, beta-turn, and so forth provide critical templates for the three-dimensional structure, but the overa... |
9275313 | Chiral recognition by the copper(II) complex of 6-deoxy-6-N-(2-methylaminopyridine)-beta-cyclodextrin. | A modified beta-cyclodextrin bearing a 2-aminomethylpyridine binding site for copper(II) (6-deoxy-6-[N-(2-methylamino)pyridine)]-beta-cyclodextrin, CDampy) was synthesized by C6-monofunctionalization. The acid-base properties of the new ligand in aqueous solution were investigated by potentiometry and calorimetry, and ... |
9275310 | Improvement of enantioselective syntheses and chiral high resolution gas chromatographic analyses of (+)-2-allyl-2-carboethoxy-cyclopentanol. | The improvement of the biocatalytic reduction of 2-allyl-carboethoxy-cyclopentanone (2) to the corresponding cyclopentanol derivative (+)-(1R,2R)-(1) was accomplished employing baker's yeast in organic media. This chiral cyclopentanol derivative (1), analyzed by high resolution gas chromatography performed over beta-cy... |
9271847 | Bacterial growth and virulence factors production by different Bordetella pertussis strains. | The aim of this study was to compare bacteria growth and pertussis antigens (pertussis toxin-PT, filamentous haemagglutinin-FHA and endotoxin-LPS) production by 11 Bordetella pertussis strains. A synthetic Stainer-Scholte culture medium supplemented with (2,6-0-dimethyl) beta-cyclodextrin (heptakis) or methylcellulose ... |
9289273 | [Elucidation of functionally active domains in molecules of protective antigen bacillus anthracis's toxin]. | Limited proteolysis has established that the protective antigen (PA) molecule consists of four functional-active domains. So, the shielding domain borrows an area in the linear structure of the PA molecule with NH2 of the end up to Lys 166 and plays a conducting role in the proteolytic activation of PA. The associative... |
9253152 | A cell-based immunobiosensor with engineered molecular recognition--Part II: Enzyme amplification systems. | Immune cells in vivo routinely perform highly selective immunosensing in blood and tissues as part of their normal immune surveillance functions. We have been investigating the potential of exploiting the immunosensing detection abilities of excitable immune cells (i.e. the mast cell) for the development of whole cell ... |
9248540 | Prehospital care, importance of early intervention on outcome. | The improvement of rescue systems and on-scene therapy has lead to a significant reduction of early posttraumatic death. It was the goal of this study to critically analyse the value of prehospital care in respect to early but also to delayed complications (single = SOF or multiple organ failure = MOF) In a retrospecti... |
9238646 | Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5. | The FLAG peptides DYKDDDDK and MDYKDDDDK are widely used affinity tags. Here we describe new variants of the FLAG peptides which, in direct ELISA, showed selective and differential binding to the commercially available anti-FLAG monoclonal antibodies M1, M2 and M5. Variants of the FLAG peptides were synthesized on poly... |
9204138 | Natural and artificial carbohydrate-glued protein aggregates. | Carbohydrate gluing (which may have a carbohydrate-lectin binding mechanism) was first recognized as a major contributor in the supramolecular assembly of annelid giant Hb from the marine-worm P. aibuhitensis. Although this assembly obviously also relies on protein-protein interactions, the authors tested the applicati... |
9204137 | Supramolecular architectures for the functionalization of solid surfaces. | Surface plasmon optical techniques are described as sensitive tools that allow for the on-line characterization of supramolecular biofunctional architectures at solid/solution interfaces. After a short introduction into the fundamentals of surface plasmon optics the observation of the build up of a functional bio-inter... |
9204134 | The influence of protein and interfacial structure on the self-assembly of oriented protein arrays. | These results demonstrate the complexity of factors that impact the self-assembly of protein arrays via the specific binding to receptor-functionalized interfaces. Both the composition and colloidal properties of the protein and target membrane surfaces will affect the protein-surface interactions. However, different s... |
9204131 | Supramolecular assembly using helical peptides. | We investigated supramolecular assemblies of various hydrophobic helical peptides. The assemblies were formed at the air/water interface or in aqueous medium. The hexadecapeptide, Boc-(Ala-Aib)s-OMe (BA16M), was reported to take alpha-helical structure by X-ray analysis. Several derivatives were prepared, which have th... |
9203358 | Sialic acids in molecular and cellular interactions. | Sialic acids (Sias) are terminal components of many glycoproteins and glycolipids especially of higher animals. In this exposed position they contribute significantly to the structural properties of these molecules, both in solution and on cell surfaces. Therefore, it is not surprising that Sias are important regulator... |
9195042 | Effect of self association of bis-ANS and bis-azo dyes on protein binding. | A correlation was found between the ability of dyes (ANS, bis-ANS, Congo red, Evans blue) to form self-associated supramolecular structures in water and their tendency to form complexes with proteins. The self-association ability of dyes was measured as the resistance of a molecular sieve to their penetration. Quantita... |
9189630 | The biomimetics of enamel: a paradigm for organized biomaterials synthesis. | The formation of enamel takes place through a sequence of processes that can be mimicked in inorganic materials chemistry. This chapter describes the generic features of enamel biomineralization in terms of a house-building analogy. Four stages are identified: supramolecular preorganization and spatial patterning; inte... |
9189622 | Tuftelin: enamel mineralization and amelogenesis imperfecta. | Tuftelin is a novel acidic enamel protein thought to play a major role in enamel mineralization. Its identity and localization has been confirmed by amino acid composition, enzyme-linked immunosorbant assay, Western blots, indirect immunohistochemistry and high resolution protein-A gold immunocytochemistry. The deduced... |
9189621 | Amelogenin proteins of developing dental enamel. | The amelogenins of developing dental enamel are tissue-specific proteins, rich in proline, leucine, histidine and glutamyl residues, and synthesized by the ameloblast cells of the inner enamel epithelium. These proteins comprise the bulk of the extracellular matrix that becomes mineralized with a hydroxyapatite phase t... |
9184756 | Origin of biochemical organization. | The problems of the origin of life and biochemical evolution are analyzed in the context of the biochemical organization concept proposed earlier by the authors. The traditional view according to which the evolution of biological systems occurred from lower levels to systems of higher levels is criticized. It is sugges... |
9179779 | Comparative studies on the interaction of proteins with a polydimethylsiloxane elastomer. II. The comparative antigenicity of primary and secondarily adsorbed IgG1 and IgG2a and their non-adsorbed counterparts. | The antigenicity of bovine IgG1 and IgG2a adsorbed on a polydimethysiloxane (PEP) elastomer, on a widely used polystyrene (Imm 2, Dynatech) or immobilized as biotinylated proteins to streptavidin covalently bound to polystyrene (SA-PS) was compared using various monoclonal (mAbs) and polyclonal antibodies (pAb) to bovi... |
9179778 | Comparative studies on the interaction of proteins with a polydimethylsiloxane elastomer. I. Monolayer protein capture capacity (PCC) as a function of protein pl, buffer pH and buffer ionic strength. | Polydimethylsiloxane (PEP) is widely used in medical prostheses and therefore is in contact with plasma and secretory proteins. Two pair of globular proteins, lactoferrin (Lf) and transferrin (Trf), and bovine IgG1 and IgG2a, which differ substantially between pair members in their pl, were used to study the interactio... |
9179777 | Crystal structure of the bovine alpha-chymotrypsin:Kunitz inhibitor complex. An example of multiple protein:protein recognition sites. | The crystal structure of bovine alpha-chymotrypsin (alpha-CHT) in complex with the bovine basic pancreatic trypsin inhibitor (BPTI) has been solved and refined at 2.8 A resolution (R-factor = 0.18). The proteinase:inhibitor complex forms a compact dimer (two alpha-CHT and two BPTI molecules), which may be stabilized by... |
9179776 | Peptide-derived self-assembled monolayers: adsorption of N-stearoyl L-cysteine methyl ester on gold. | The work reported herein concerns the assembly of N-stearoyl L-cysteine methyl ester [CH3(CH2)16COCysOMe, 1] on the surface of gold. This compound serves as a simple model of a related polypeptide, which has been designed to adopt a beta-sheet architecture on metallic and oxide surfaces. We describe the preparation of ... |
9179775 | Detection of mutations in PCR products from clinical samples by surface plasmon resonance. | Two different strategies for scanning and screening of mutations in polymerase chain reaction (PCR) products by hybridization analysis are described, employing real-time biospecific interaction analysis (BIA) for detection. Real-time BIA was used to detect differences in hybridization responses between PCR products and... |
9179774 | Structural studies of spider silk proteins in the fiber. | Although spider silk has been studied for a number of years the structures of the proteins involved have yet to be definitely determined. X-ray diffraction and solid-state nuclear magnetic resonance (NMR) were used to study major ampullate (dragline) silk from Nephila clavipes. The silk was studied in its natural state... |
9178514 | A cell-based immunobiosensor with engineered molecular recognition--Part I: Design feasibility. | A novel bioelectronic sensor is described in which living immune cells are transformed into unique biotransducer couples by engineering their molecular recognition for preselected antigens of clinical interest. This 'hybrid' biosensor, constructed with mast cells interfaced to a microfabricated thermoelectric device wi... |
9169988 | Release of amphotericin B from delivery systems and its action against fungal and mammalian cells. | Spectroscopic studies of four amphotericin B (AmB) lipid preparations--small negatively charged unilamellar vesicles "AmBisome", positively charged oligolamellar liposomes "Ampholiposomes", AmB Lipid Complex "L-AmpB33"--and AmB association with gamma cyclodextrin demonstrated that the composition of drug delivery syste... |
9151192 | Synthesis and characterization of biodegradable polyrotaxane as a novel supramolecular-structured drug carrier. | Polyrotaxanes were synthesized as novel biodegradable polymers with supramolecular assembly and their properties evaluated in vitro. The synthesis of biodegradable polyrotaxanes consists of three steps: preparation of an inclusion complex consisting of alpha-cyclodextrins (alpha-CDs) and amino-terminated poly(ethylene ... |
9149432 | Effect of cyclodextrins on the hydrolysis of an oxazolidine prodrug of (1R,2S)-(-)-ephedrine-cis-2-(4-methoxyphenyl)-3, 4-dimethyl-5-phenyloxazolidine. | Molecular complexes of an oxazolidine prodrug of (-)-ephedrine, cis-2-(4-methoxyphenyl)-3,4-dimethyl-5-phenyloxazolidine, with alpha-, beta-, dimethyl-beta- (DM-beta-) and hydroxypropl-beta- (HP-beta-) cyclodextrins (CDs) were examined using ionspray mass spectrometry. The results suggest, under our experimental condit... |
9134696 | Chiral recognition in liquid chromatography utilising chargeable cyclodextrins for resolution of doxazosin enantiomers. | The chromatographic resolution of rac-doxazosin using reversed-phase high performance liquid chromatography (HPLC) with the chargeable chiral mobile phase additive, carboxymethyl-beta-cyclodextrin (CM-beta-CD), is described. The effects of different modifiers (acetonitrile, methanol and tetrahydrofuran), pH, temperatur... |
9131821 | Analysis of glycosidic linkages in saccharide compounds by post-source decay fragment methods in matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy. | Maltotriosyl- and panosyl-alpha-cyclodextrins and the nonaose of pullulan were analyzed by post-source decay (PSD) fragment methods of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectroscopy. By the mass number analysis, it was found that all of the PSD fragment ions were produced by cl... |
9127952 | Basement-membrane stromal relationships: interactions between collagen fibrils and the lamina densa. | Collagens, the most abundant molecules in the extracellular space, predominantly form either fibrillar or sheet-like structures-the two major supramolecular conformations that maintain tissue integrity. In connective tissues, other than cartilage, collagen fibrils are mainly composed of collagens I, III, and V at diffe... |
9112756 | Investigating synthetic P-regions from voltage-gated sodium channel at the conformational and functional levels. | Four peptides mimicking the four P-regions of the electric eel sodium channel were chemically synthesized to characterize their secondary structure and their contribution to the channel selectivity. Circular dichroism spectra of these peptides in trifluoroethanol demonstrate an important beta-sheet conformational compo... |
9112070 | Recent studies on dendrotoxins and potassium ion channels. | 1. Dendrotoxins are small proteins isolated from mamba (Dendroaspis) snake venoms. They block some subtypes of voltage-dependent potassium channels in neurons. 2. Dendrotoxins contain 57-60 amino acid residues crosslinked by three disulfide bridges. They are homologous to Kunitz-type serine protease inhibitors, such as... |
9083625 | Advanced fluorocarbon-based systems for oxygen and drug delivery, and diagnosis. | Fluorocarbons and fluorocarbon-derived materials constitute a vast family of synthetic components that have a range of remarkable properties including exceptional chemical and biological inertness, gas-dissolving capacity, low surface tension, high fluidity, excellent spreading characteristics, unique hydro- and lipoph... |
9080479 | Biodistribution of model 105Rh-labeled tetradentate thiamacrocycles in rats. | 105Rh(III)Cl2 complexes with a limited series of [14]ane- and [16]ane- thia macrocycles were prepared and their biodistributions in Sprague-Dawley rats studied. These studies demonstrate that modifications in the structure and composition of the 105Rh-thia macrocycle complexes produce significant differences in their u... |
9181798 | [Interpretation of the resonance Raman spectrum of cytochrome P-450]. | Normal coordinate analysis has been carried out for the Fe(III) protoporphyrin IX dimethyl ester molecule and its vinyl deuterated analogs. All atoms of substituents were included explicitly in the calculations. On the basis of the calculation results the assignment of the resonance Raman active modes of cytochrome P-4... |
9076999 | Observation of non-covalent interactions between beauvericin and oligonucleotides using electrospray ionization mass spectrometry. | Electrospray ionization mass spectrometry has been used to study the possible non-covalent interaction between oligonucleotides and beauvericin (B) mycotoxin. Beauvericin-oligonucleotide adduct formation was observed even at low mycotoxin concentration (25 pmol/microL). Adducts were found with different numbers of B li... |
9148724 | Ultrastructural analysis of the vestibular nerve in Ménière's disease. | Numerical analysis of the vestibular nerve in Méniè's disease was performed. Vestibular nerve specimens were obtained from three patients with classical clinical findings of Ménière's disease during retrosigmoid vestibular neurectomy. For each patient, an ultrathin section, including an entire cross-section of the nerv... |
9062414 | Lack of correlation between thymidylate synthase levels in primary colorectal tumours and subsequent response to chemotherapy. | The increasing interest in 5-fluorouracil (5-FU) modulation and the development of new antifolates has focused attention in recent studies on the expression of the target enzyme thymidylate synthase (TS) as a determinant of drug sensitivity and resistance. Resistance to TS-directed drugs has been shown to occur in vitr... |
9072306 | Enhancement of cellular immunity in melanoma patients immunized with a peptide from MART-1/Melan A. | <h4>Purpose</h4>In this study, we tested the effectiveness of a melanoma-associated antigen-derived peptide, MART-1(27-35), in eliciting cellular immune responses in vivo in the context of a phase I active immunization protocol. This peptide (AAGIGILTV) corresponds to residues 27-35 from the nonmutated melanoma-associa... |
9051732 | Protein engineering tests of a homology model of Plasmodium falciparum lactate dehydrogenase. | This paper describes the testing of a homology model of Plasmodium falciparum lactate dehydrogenase (pfLDH) by protein engineering. The model had been validated in structural terms. It suggests explanations of the unusual properties of pfLDH (compared with all other LDHs). These unusual features are a lack of substrate... |
9051207 | Direct chiral resolution of phenylalkylamines using a crown ether chiral stationary phase. | A direct, isocratic high-performance liquid chromatographic method is described for the enantiomeric resolution of a number of phenylalkylamines, namely, racemic cathinone, amphetamine, norephedrine, and norphenylephrine, without sample derivatization. The separations were achieved on an S-18-crown-6-ether chiral stati... |
9051206 | Systematic multivariate optimization of separation of auxins by capillary electrophoresis based on overlapping resolution mapping scheme. | In this paper, the overlapping resolution mapping (ORM) scheme in the separation of 15 plant auxins by capillary electrophoresis. An interpretative optimization approach was utilised to predict the optimum buffer pH, and the concentrations of alpha-, beta-, and gamma-cyclodextrins. A four-dimensional overlapping resolu... |
9133103 | [Acute pancreatitis--conservative versus surgical therapy]. | Based on own studies and on results of other centers the multiorgan failure (MOF) resp. the systemic inflammatory response syndrome (SIRS) in acute necrotizing pancreatitis seems to be no longer an compelling reason for surgery in the absence of proven infection of pancreatic necrosis. Growing experience of continuous ... |
9043816 | Cyclodextrins--enabling excipients: their present and future use in pharmaceuticals. | Cyclodextrins (CDs) complex hydrophobic drugs, increasing their aqueous solubility and stability. CD complexation enables the creation of formulations for water-insoluble drugs that are difficult to deliver with more traditional formulations. Currently, 10 pharmaceutical products are marketed as CD formulations. A CD-b... |
9038014 | [The role of surgery for chronic empyema of the advanced ages]. | Chronic empyema, a sequelae of pulmonary tuberculosis, is now a only tuberculosis-related disease which was remained to be treated surgically. The candidates who have basically poor respiratory function are now attained advanced age. Over a 15 years period (1980-95), 22 patients 70 years of age or older underwent surgi... |
9034218 | Tensioactivity and supramolecular organization of the palmitoyl prodrug of timolol. | <h4>Purpose</h4>To improve the bioavailability of the ocular drug timolol by facilitating its transport through the cornea, an amphiphilic prodrug was synthesized via the addition of a palmitic chain by esterification. The present study was undertaken to investigate the physicochemical and tensioactive properties of th... |
9034203 | Hypocholesterolemic action of beta-cyclodextrin and its effects on cholesterol metabolism in pigs fed a cholesterol-enriched diet. | To examine the effects of beta-cyclodextrin (BCD), a non-absorbable carbohydrate, on lipid metabolism, growing pigs were fed a 0.3% cholesterol-enriched diet for 4 weeks or this diet containing 5% or 10% BCD. Pigs fed a basal diet without added cholesterol or BCD were used as controls. The cholesterol-rich diet induced... |
9030197 | Improved oligonucleotide uptake and stability by a new drug carrier, the SupraMolecular Bio Vector (SMBV). | Antisense oligodeoxynucleotides are potential therapeutic agents, but their development is still limited by both a poor cellular uptake and a high degradation rate in biological media. The strategy that we propose to face these problems is to use small synthetic carriers, around 30 nm diameter, the SupraMolecular Bio V... |
9029726 | Molecular recognition and electron transfer in mitochondrial steroid hydroxylase systems. | Mitochondrial monooxygenase systems are involved in the biosynthesis of glucocorticoids, mineralocorticoids, bile acids, and 1,25-dihydroxyvitamin D. The reactions are catalyzed by specific P450 enzymes that receive reducing equivalents via NADPH-ferredoxin oxidoreductase (adrenodoxin reductase) and ferredoxin (adrenod... |
9029725 | P450BM-3; a tale of two domains--or is it three? | Over 400 P450s have been identified to date in prokaryotes and eukaryotes, plants and animals, mitochondria and endoplasmic reticulum. These enzymes function in areas such as metabolism and steroidogenesis. The eukaryotic members of this gene superfamily of proteins have proved difficult to study because of the hydroph... |
9029322 | Determination of a basic drug, bambuterol, in human plasma by capillary electrophoresis using double stacking for large volume injection and supported liquid membranes for sample pretreatment. | In this work we show the potential of using a double stacking procedure based on field enhancement as a means to increase the concentration sensitivity in CZE analysis of human plasma extracted by the supported liquid membrane (SLM) technique. A basic drug, bambuterol, was used as a model substance. The low ionic stren... |
9029143 | Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation. | Small heat shock proteins (sHsps) are a conserved and ubiquitous protein family. Their ability to convey thermoresistance suggests their participation in protecting the native conformation of proteins. However, the underlying functional principles of their protective properties and their role in concert with other chap... |
9116113 | Complications of planned relaparotomy in patients with severe general peritonitis. | <h4>Objective</h4>To analyse the complications of planned relaparotomy for severe general peritonitis and to define when to discontinue relaparotomies.<h4>Design</h4>Retrospective study.<h4>Setting</h4>University hospital, The Netherlands.<h4>Subjects</h4>24 consecutive patients who underwent planned relaparotomy for w... |
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