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A flow-injection biosensor system with an on-line microdialysis sampling is proposed for the simultaneous assay of l-lactate and pyruvate in serum and rat brain. The dialysate collected in the sample loop by perfusing Ringer's solution through the microdialysis probe is automatically injected into the flow-injection li... |
A series of hyperbranched poly(amine-ester)s based on 1,1,1-trimethylolpropane, methyl acrylate and diethanolamine were synthesized and coated on the inner surface of the fused-silica capillaries by physical adsorption. The most effective coating was the seventh generation hyperbranched poly(amine-ester) coating, which... |
The cell lines chosen have demonstrated a positive response in terms of cell proliferation and associated modifications in proteins content, evaluated through DNA and proteins synthesis, at environmentally relevant dose of dioxins, brought by a typical environmental PCDD/F mixture. The response is time and species depe... |
Analyses of dioxins in food have become increasingly important since the European Commission has enforced maximal toxic equivalent concentration (TEQ) levels in various food and feed products. Screening methodologies are usually used to exempt those samples that are below the maximum permitted limit and that can, there... |
Polyhalogenated aromatic hydrocarbons, such as polychlorinated dibenzo-p-dioxins are a large and diverse group of environmental pollutants. Their tendency to accumulate in the food chain and their toxicity make monitoring necessary. The reference analysis method is laborious and very expensive, therefore cheap and rapi... |
Stabilisation of electrochemically deposited Prussian blue (PB) films on glassy carbon (GC) electrodes has been investigated and an enhancement in the stability of the PB films is reported if the electrodes are treated with tetrabutylammonium toluene-4-sulfonate (TTS) in the electrochemical activation step following th... |
The use and application of synthetic zeolites for ion exchange, adsorption and catalysis has shown enormous potential in industry. In this study, X-ray fluorescence (XRF) analysis was used to determine Si and Al in fly ash (FA) precipitates. The Si and Al contents of the fly ash precipitates were used as indices for th... |
An amperometric biosensor was prepared by in situ deposition of horseradish peroxidase (HRP) enzyme on a polyaniline (PANI)-doped platinum disk electrode. The PANI film was electrochemically deposited on the electrode at 100mVs(-1)/Ag-AgCl. Cyclic voltammetric characterization of the PANI film in 1M HCl showed two dist... |
Immunosensors and biochemical array detection systems based on electrochemical transducers have many advantages such as low detection limit, fast response, simple design and ease of miniaturization. However, further development of such sensors will depend on the availability of suitable substrates that can be converted... |
A new method for the fluorometric determination of zearalenone (ZEN) based on its reaction with betaNADH in the presence of the enzyme 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD) is described. The procedure is based on the change in fluorescence intensity that takes place during the enzymatic reaction (excitation ... |
The use of 3-indoxyl phosphate (3-IP) as an electrochemical substrate for ELISAs with voltammetric detection was investigated. Indirect measurements of alkaline phosphatase (AP) and horseradish peroxidase (HRP) activity in solution were carried out. Picomolar levels of both enzymes can be detected, which enables the de... |
A new, highly sensitive, fast responding and stable potentiometric biosensor for creatinine determination is developed. The biosensor is based on an ammonium ion-selective electrode. Creatinine deiminase (EC 3.5.4.21) is chemically immobilized on the surface of the polymeric ion-sensitive membrane in the form of monomo... |
Three cholesterol biosensor configurations based on the formation of a layer of Prussian-Blue (PB) on a Pt electrode for the electrocatalytic detection of the H(2)O(2) generated during the enzymatic reaction of cholesterol with cholesterol oxidase (ChOx) were constructed. The enzyme was entrapped within a polypyrrole (... |
A flow-injection spectrophotometric method has been developed for the simultaneous determination of selenium(IV) and (IV + VI) at nanogram per milliliter levels. It is based on the catalytic effect of selenium(IV) on the photooxidative coupling of p-hydrazinobenzenesulfonic acid (HBS) with N-(1-naphthyl)ethylenediamine... |
An acid urease column was applied to a fluorometric flow-injection analysis (FIA) system as a recognition element for determination of urea in rice wines. The acid urease has specific properties of showing its catalytic activity in low pH range and tolerance to ethanol in comparison to those of a urease from jack-beans... |
An electrode for glucose has been prepared by using an iodide selective electrode with the glucose oxidase enzyme. The iodide selective electrode used was prepared from 10% TDMAI and PVC according our previous study. The enzyme was immobilized on the iodide electrode by holding it at pH 7 phosphate buffer for 10min at ... |
The possibility of using moss (Funaria hygrometrica), immobilized in a polysilicate matrix as substrate for speciation of Cr(III) and Cr(VI) in various water samples has been investigated. Experiments were performed to optimize conditions such as pH, amount of sorbent and flow rate, to achieve the quantitative separati... |
In this study, a flow injection analysis (FIA) system using a cartridge of immobilized isocitrate lyase (ICL) and isocitrate dehydrogenase (ICDH) was developed to monitor the concentrations of succinic acid in biotechnological processes. The ICL and ICDH immobilized on VA-Epoxy Biosynth E3-carrier had a good operationa... |
Simple and sensitive method was developed for the spectrophotometric determination of tungsten. The method was based on the formation of the colour associate of molybdotungsten isopolyanion (IPA) with organic dye malachite green and solubilization in the solution of non-ionic surface-active substance (SAS). Recommended... |
In this paper, it is demonstrated that a single-receptor biosensor can be used to quantitatively determine each analyte in binary mixtures using multivariate data analysis tools based on the dynamic responses received from flow injection peaks. Mixtures with different concentrations of two phenolic compounds, catechol ... |
Electrochemical glucose enzyme biosensors have been prepared on carbon film electrodes made from carbon film electrical resistors. Evaluation and characterisation of these electrodes in phosphate buffer saline solution has been carried out with and without pretreatment by cycling in perchloric acid or at fixed applied ... |
A tandem technique for the detection of very low levels E. coli within about 2h is demonstrated. The technique couples the widely employed microbial enzymatic detection methods with an immunoassay step. The bacterial marker enzyme, E. coli beta-D-galactosidase, was used in conjunction with synthetic enzyme substrates t... |
The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase, peroxidase, acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticide... |
Immunoenzyme assay (ELISA) is proposed and characterized for determination of alkylphenol ethoxylates, a primary class of manufactured non-ionic surfactants. The assay is based on the obtained polyclonal antibodies against nonylphenol (NP), the main stable intermediate of the decomposition of nonylphenol ethoxylates. A... |
Luminescent particles of lead carboxymethyl cellulose (Pb(CMC)(2)), which contains salicyl fluorones (THBF), Pb(CMC)(2)-THBF, were synthesized by sol-gel method. Pb(CMC)(2)-THBF can emit intense and stable solid substrate room temperature phosphorescence (SS-RTP) on filter paper. EDTA can chelate the Pb(2+) in Pb(CMC)(... |
Voltammetric enzyme immunosensors based on the employment of streptavidin modified screen-printed carbon electrodes (SPCEs) for the detection of rabbit IgG, as a model analyte, were described. Alkaline phosphatase (AP) and 3-indoxyl phosphate (3-IP) were used as the enzymatic label and substrate, respectively. The adso... |
In the present paper a focused-microwave Kjeldahl digestion procedure without metal catalyst for nitrogen determination in bean samples was developed. Temperature at which the decomposition plateau occurs, mass of potassium sulphate and either volume of sulphuric acid or hydrogen peroxide were optimised. Results of the... |
In this work a method is presented for the enzymatic determination of glucose using fluorescence anisotropy. During the enzymatic reaction a change in the fluorescence anisotropy of the glucose oxidase (GOx) is produced; the reaction time at which this change appears (t(m)) depends on the glucose concentration. A theor... |
In the work described here, a biosensor was developed for the determination of sulfite in food. Malva vulgaris tissue homogenate containing sulfite oxidase enzyme was used as the biological material. M. vulgaris tissue homogenate was crosslinked with gelatin using glutaraldehyde and fixed on a pretreated Teflon membran... |
A method is described for the evaluation of the peroxide value (PV, meq. O(2) kg(-1)) in olive oil. The method is based on the chemiluminogenic energy-transfer reaction of bis(2,4,6-(trichlorophenyl)oxalate (TCPO) with hydrogen peroxide or total peroxides in the presence of Mn(II) as catalyst and 9,10-dimethylanthracen... |
The performance of amperometric glucose biosensors based on the dispersion of glucose oxidase (GOx) and copper oxide within a classical carbon (graphite) paste composite is reported in this work. Copper oxide promotes an excellent electrocatalytic activity towards the oxidation and reduction of hydrogen peroxide, allow... |
Sequential injection-bead-based immunoassay system has been developed. The main purpose is to make immunoassay process more automated by manipulating the precise delivery of micro-volumes of reagents and the precise timing of incubation and washing steps with a computer program that controls the bi-directional syringe ... |
In this work quantum-sized CdS nanocrystals were synthesized using a quaternary water-in-oil microemulsion and immobilized onto gold working electrode by self-assembled monolayers techniques. Formaldehyde dehydrogenase was covalently immobilized onto a protecting membrane, which was stratified on part of the semiconduc... |
Chirasil-beta-Dex containing an undecamethylene spacer (C11-Chirasil-Dex) was used as chiral stationary phase (CSP) in enantioselective gas chromatography (GC). The versatility of the new stationary phase in the simultaneous enantiomeric separation of a set of cyclopropane derivatives is demonstrated. The GC method pro... |
A highly selective, fast and stable biosensor for determination of glucose in soluble coffee has been developed. The biosensor electrode consist of a thin film of ferric hexacyanoferrate (Prussian Blue or PB) electrodeposited on the glassy carbon electrode (GCE) (to provide a catalytic surface for the detection of hydr... |
Anthropogenic emission of platinum group elements (PGEs) from the abrasion of automotive catalytic converters into the environment has significantly increased. Dust emitted from the catalyst is causing pollution problems of these metals in the future. However, the concentration level of these PGEs is still very low in ... |
This research has evaluated the ability of cross-flow filtration (CFF) to perform correct size fractionation of natural aquatic colloids (materials from 1nm to 1mum in size) and particles (>1mum) using scanning electron microscopy (SEM) combined with atomic force microscopy (AFM). SEM provided very clear images at high... |
To improve the antigen-binding activity of liposome-coupled antibodies and to develop universal liposomal nanovesicles for immunoassays, protein G was conjugated to dye-loaded liposomal nanovesicles for the preparation of immunoliposomes. Sulfosuccinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC), a... |
A simple, rapid, and highly sensitive immunosensor for the direct determination of carcinoembryonic antigen (CEA) in human serum using a piezoelectric crystal has been developed and optimized. In order to improve sensitivity of the immunosensor, a protein A-based orientation-controlled immobilization method for antibod... |
The use of aerosol defense sprays has increased as a means of self-defense and as a weapon in the commission of a crime. The residue of these sprays is often left behind as physical evidence on a victim's clothing or personal belongings. As the popularity of self-defense weaponry increases, so does the likelihood that ... |
The nature of art lends itself to forgery as a skilled and determined forger can mimic the techniques and styles of an artist to a level where even an expert can be duped. The authentication of paintings is a subjective process, but modern techniques may provide the means to provenance artist pigments based on elementa... |
This paper reviews recent developments in the design and application of two types of optical nanosensor, those based on: (1) localized surface plasmon resonance (LSPR) spectroscopy and (2) surface-enhanced Raman scattering (SERS). The performance of these sensors is discussed in the context of biological and chemical s... |
CdSe nanocrystals, also called quantum dots (Qdots) are a novel class of fluorophores, which have a diameter of a few nanometers and possess high quantum yield, tunable emission wavelength and photostability. They are an attractive alternative to conventional fluorescent dyes. Quantum dots can be silanized to be solubl... |
This paper reports an improved catalytic molecular beacon. Addition of the target oligonucleotide activates a DNA enzyme (DNAzyme), which, in turn, activates multiple copies of molecular beacons (MB) and gives rise to a strong fluorescence signal. In a previous design, the activated DNAzyme could oligomerize, especiall... |
This work reports a hybrid flow-injection analysis (FIA)/sequential-injection analysis (SIA) method for the rapid enzymatic assay of glucose with soluble glucose oxidase (GOD). The method relies on the sequential injection of segments of the sample and of a solution of enzyme by means of a multi-port selection valve in... |
There is a need to develop reliable portable analytical systems for on-site and real-time biomonitoring of lead (Pb) from both occupational and environmental exposures. Saliva is an appealing matrix since it is easily obtainable, and therefore a potential substitute for blood due to existing reasonably good correlation... |
The elimination or minimization of non-specific protein adsorption from serum is critical for the use of surface plasmon resonance (SPR) sensors for in vitro and in vivo analysis of complex biological solutions. The ultimate goals in this application are to minimize non-specific adsorption of protein and to maximize an... |
A simple reversed-phase high-performance liquid chromatographic method employing C-18 column has been developed for simultaneous analysis of three intermediates in the synthesis of S-duloxetine, the antidepressant drug, viz., 2-acetyl thiophene (AT), N,N-dimethyl-3-keto-(2-thienyl)-propanamine (DKTP) and (S)-N,N-dimeth... |
A rapid and sensitive method is described for the determination of scopolamine and its metabolites in rat urine by combining liquid chromatography and tandem mass spectrometry (LC-MS/MS). Various extraction techniques (free fraction, acid hydrolyses and enzyme hydrolyses) and their comparison were carried out for inves... |
The preparation and characterization of an amperometric glucose biosensor based on the entrapment of glucose oxidase (GOx) in a polyacrylamide microgel is described. This study proves that polyacrylamide microgels provide an excellent matrix for GOx immobilization that can be used as a biological material in amperometr... |
Carbon film resistor electrodes have been evaluated as transducers for the development of multiple oxidase-based enzyme electrode biosensors. The resistor electrodes were first modified with Prussian Blue (PB) and then covered by a layer of covalently immobilized enzyme. Electrochemical impedance spectroscopy was used ... |
A polyclonal antibody against trinitrophenyl (TNP) derivatives was raised in rabbit, and the antibody was applied to detection of trinitrotoluene (TNT) using a surface plasmon resonance (SPR) biosensor. TNP-keyhole limpet hemocyanine (TNP-KLH) conjugate was injected into a rabbit, and a polyclonal anti-TNP antibody was... |
A pre-column derivatization method for the sensitive determination of amino acids and peptides using the tagging reagent 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) followed by high-performance liquid chromatography with fluorescence detection has been developed. Identification of derivatives was carri... |
Speciation of mercury in salmon egg cell cytoplasm was investigated by surfactant-mediated high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS), where an ODS (octadecylsilica) column coated with a bile acid derivative, CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1-propane... |
The purpose of this work was the development of a method for the determination of Se compounds in leaves of plants. Water-soluble Se compounds were extracted from samples by water. Enzymatic hydrolysis with the non-specific enzyme protease XIV was used for the release of Se compounds bound to proteins. Separation of Se... |
A wide size range of SiO(2) particles were synthesized and were used as enzyme immobilization carriers to fabricate glucose biosensors. The size of the particles was in the range of 17-520 nm. These biosensors could be operated under physiological conditions (0.1M phosphate buffer, pH 7.2). Particle size could affect t... |
The monodisperse, 5.0 microm non-porous poly(glycidylmethacrylate-co-ethylenedimethacrylate) (P(GMA/EDMA)) beads were prepared by a single-step swelling and polymerization method. The seed particles prepared by dispersion polymerization exhibited good absorption of the monomer phase. Based on this media, a weak cation ... |
Eighteen-nanometer gold and 3.5-nm silver colloidal particles closely packed by cetyltrimethylammonium bromide (CTAB) to form its positively charged shell. The DNA network was formed on a mica substrate firstly. Later, CTAB-capped gold or silver colloidal solutions were cast onto DNA network surface. It was found that ... |
A new amperometric biosensor for hydrogen peroxide was developed based on cross-linking horseradish peroxidase (HRP) by glutaraldehyde with multiwall carbon nanotubes/chitosan (MWNTs/chitosan) composite film coated on a glassy carbon electrode. MWNTs were firstly dissolved in a chitosan solution. Then the morphology of... |
A COD measurement by a photocatalytic oxidation method using nano-TiO(2) film was investigated. K(2)Cr(2)O(7) was added into the solution to enhance the efficiency of photocatalytic degradation, and simultaneously K(2)Cr(2)O(7) was reduced to Cr(III) by photogenerated electrons, which were adsorbed on the surface of Ti... |
The use of several designs of amperometric enzymatic biosensors based on the immobilized tyrosinase enzyme (Tyr) for determining dichlorvos organophosphate pesticide are described. The biosensors are based on the reversible inhibition of the enzyme and the chronocoulometric measurement of the charge due to the charge-t... |
A new kind of near-infrared fluorescence agent, tricarbochlorocyanine dye (Cy.7.Cl), had been synthesized in house and used for near-infrared spectrofluorimetric determination of hydrogen peroxide (H(2)O(2)) by flow injection analysis (FIA) for the first time. The oxidation reaction of Cy.7.Cl with H(2)O(2) occurred un... |
A detection of alkaline phosphatase (ALP, EC 3.1.3.1) activity by the monitoring of pH changes caused by the biocatalytic action of the enzyme has been experimentally examined. Enzymatically catalyzed hydrolysis of monofluorophosphate has been found to be the best basis for such measurements. Protolytic equilibria conn... |
Wavelength modulation surface plasmon resonance biosensors (SPR) using colloidal Au nanoparticles and double-linker sensing membrane enhancement are reported for determination of transferrin. The 2-mercaptoethylamine (MEA) was immobilized on the biosensor surface with traditional amine coupling method. The interaction ... |
Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor s... |
A mediator-free amperometric biosensor for screening organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system based on anticholinesterase activity of OPs to immobilized acetylcholinesterase enzyme (AChE) has been developed. The enzyme biosensor is prepared by entrapping AChE in Al(2)O(3) sol-gel matrix... |
An electrode covered with a lectin/collagen film was constructed to investigate whether the film was usable as a reaction field of binding between the lectin and sugar. The protein-sugar binding on cell surface plays an important role to various physiologic processes. The film is considered to be a cell surface, due to... |
Ligand-tagged liposomes, obtained by covalent conjugation of ligands to the liposomal surface, have been widely used as detection reagents in bioanalytical assays. A non-covalent conjugation method where IgG was attached to protein G-tagged liposomes has been recently utilized. To enlarge the application of non-covalen... |
A reagentless amperometric uric acid biosensor based on zinc sulfide (ZnS) quantum dots (QDs) was firstly developed. It could detect uric acid without the presence of an electron mediator. The carboxyl group functionalized ZnS QDs were synthesized, and they were soluble biocompatible and conductive. ZnS QDs conjugates ... |
The use of the RP-HPLC official method of the International Honey Commission (IHC) for the determination of 5-hydroxymethylfurfural (HMF) in strawberry tree honey (Arbutus unedo, a typical Sardinian honey) has brought to light a specific and heavy chromatographic interference that prevents accurate quantification. The ... |
The response characteristics of various carbon substrates towards the direct oxidative measurement of urate and other key purine biomarkers have been compared. A novel carbon fibre laminate assembly has been proposed as an alternative substrate for the preparation of disposable sensing strips. The fabrication method is... |
With lower limits of detection and increased stability constantly being demanded of biosensor devices, characterisation of the constituent layers that make up the sensor has become unavoidable, since this is inextricably linked with its performance. This work describe the optimisation and characterisation of two aspect... |
Total protein assay was made using copper(II)-neocuproine (Nc) reagent in alkaline medium (with the help of a hydroxide-carbonate-tartarate solution) after 30min incubation at 40 degrees C. The absorbance of the reduction product, Cu(I)-Nc complex, was recorded at 450nm against a reagent blank. The absorptivity of the ... |
A very simple, highly selective and sensitive assay of proteins based on the biuret absorption in the ultraviolet region has been developed. The well-known biuret assay is based on the reaction of proteins with copper ions under strong alkaline conditions to form a copper-protein complex. Yet, copper ions may seriously... |
A new fluorometric enzyme immunoassay for 17beta-estradiol (E2) using biotinylated estradiol (BE) as a probe ligand, is described. In this method, E2 is detected indirectly by a solid-phase avidin-biotin binding assay, in which the biotin is immobilized on a microtiter plate (biotin-plate). After the competitive reacti... |
A sensitive procedure for the quantification of total protein bovine serum albumen (BSA) in human serum was presented with sequential injection sampling and fluorometric detection. A few microliters of sample and fluorescamine solutions were aspirated into the holding coil to facilitate the reaction of protein with flu... |
A new strategy to construct amperometric immunosensor for human IgG assay based on ZnO/chitosan composite as sensing platform has been described. This material, which combined the advantages of inorganic species, ZnO and organic polymer, chitosan, can maintain biological activity well. A sequential sandwich immunoassay... |
Sol-gel derived tetraethylorthosilicate (TEOS) films were prepared by spin coating method on indium tin oxide (ITO) coated glass plate. Hydrophobic interaction method was used to coat the bovine serum albumin film over the surface of tetraethylorthosilicate sol-gel film to minimize cracking, biofouling and to improve t... |
An optical biosensor consisting of a chromoionophore (ETH5294) (CM) doped sol-gel film interfaced with another sol-gel film immobilized with acetylcholinesterase (AChE) was employed to detect the insecticide dichlorvos. The main advantage of this optical biosensor is the use of a sol-gel layer with immobilized CM that ... |
Metal speciation analysis in MTs was carried out in two tropical fish species of Brazil, the freshwater fish pearl cichlid (Geophagus brasiliensis) and the marine fish white sea catfish (Netuma barba), that are presently used to monitor the effects of heavy metal pollution in aquatic ecosystems in Brazil. In order to o... |
An integrated light emitting diode (LED)-induced fluorescence detector was described and evaluated. The LED and its related components including lens and interference filter, the optical fiber used to collect fluorescence, and the capillary column are integrated into a substrate block, which eliminates the need of alig... |
This paper describes novel electrochemical properties of gold nanoparticles/alkanedithiol conductive films and their electroanalytical applications for voltammetric determination of trace amount of one kind of environmental pollutants, catechol. The conductive films are prepared by closely packing 12-nm diameter gold n... |
An on-line simple and rapid method for the simultaneous determination of tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DC) residues in fish muscle was developed by coupling in-tube solid-phase microextraction (SPME) to high-performance liquid chromatography (HPLC) with a photodiode ... |
In this work, a new kinetic method was proposed for quantification phenoxyl radicals generated in enzyme reaction. Instead of direct detecting the spectral signals of phenoxyl radicals, a molecular probe, the reduced form of nicotinamide adenine dinucleotide (NADH), was employed to indicate the formation of phenoxyl fr... |
Based on the multidomain structure of Pseudomonas aeruginosa exotoxin A, a fusion protein termed rPEA has been constructed, which is expected to serve as a gene carrier in vitro. The expression and purification of rPEA are described. The basal properties of rPEA as a gene carrier are evaluated by investigating its inte... |
The development of an optical biosensor based on immobilization of 3-methyl-2-benzothiazolinone hydrazone (MBTH) in hybrid nafion/sol-gel silicate film and tyrosinase in chitosan film for the detection of phenolic compounds has been described. Tyrosinase was immobilized in chitosan film deposited on the hybrid nafion/s... |
It is difficult to detect glucose by surface-enhanced Raman spectroscopy (SERS) due to the small normal Raman cross-section and the weak adsorption of glucose molecules on the surface of noble metal. A simple and fast method is proposed in this paper for the detection of glucose based on SERS signal of the enzyme react... |
Polycarbonate-a thermostable polymer is activated by a simple and rapid method using a photolinker, 1-fluoro-2-nitro-4-azidobenzene (FNAB) for covalent immobilization of a biomolecule. Horseradish peroxidase (HRP) is used as a model enzyme to check the efficacy of the activated surface. HRP is immobilized on the activa... |
The fluorescence and solid substrate room temperature phosphorescence (SS-RTP) properties of Pd(II) meso-tetrakis (4-N-methyl-pyridiniumyl) porphyrin (Pd(II)TMPyP) were studied. The factors influencing the SS-RTP emission, such as filter type, inorganic salt sort, drying temperature, pre-drying time and drying time wer... |
We describe in this report an economic approach to construct a multiplexed capillary electrophoresis (CE) system with optic-fiber UV detection. To demonstrate the feasibility of this approach, a seven-lane CE setup is built. The system has a background noise of approximately 10(-5) absorbance unit, a limit of detection... |
The use of H(2)O(2) and UV irradiation to remove organic ligands in a chromium(III) complex for the subsequent chromium analysis is reported. The Advanced Oxidation Process (AOP) using a 5.5-W UV lamp, H(2)O(2) and Fe(2+)/Fe(3+) as catalyst (photo Fenton process) was found to give complete and quantitative Cr(III)-->Cr... |
The pheromone-binding protein from the silkworm moth, Bombyx mori (BmorPBP) has been covalently bonded to a liquid chromatographic stationary phase. The resulting column was evaluated using radiolabeled bombykol and the immobilized protein retained its ability to bind this ligand. The data also demonstrate that the Bmo... |
A kinetic method for the determination of cysteine on flow injection system is described. Cysteine was found as a catalyst for the complexation reaction of Cu(II) with an ultra sensitive colorimetric reagent of 5,10,15,20-tetrakis (4-N-trimethylammino-phenyl) porphyrin (TTMAPP) (varepsilon=4.8x10(5)cm(-1)M(-1) at 433nm... |
A modified three-step sequential extraction procedure for the fractionation of heavy metals, proposed by the Commission of the European Communities Bureau of Reference (BCR) has been applied to the Slovak reference materials of soils (soil orthic luvisols, soil rendzina and soil eutric cambisol), which represent pedolo... |
Analysis of nitrosamine (NA) standards in a model system was carried out by extraction using SPME coupled to a direct extraction device (DED) and subsequent GC/MS in selected ion monitoring mode. Gelatine (20%, w/v) systems of a NA standard (10mugL(-1)) were prepared, in order to mimic food protein matrix systems such ... |
A new matrix separation/preconcentration method is developed for the on-line determination of palladium(II) and platinum(IV) in complex matrices using a sequential ICP-OES instrument. These metals are preconcentrated in a microcolumn packed with Metalfix-Chelamine, a polymeric functionalised resin containing the tetrae... |
The synthesis of CH(2)CHCH(2)OCH(2)[15-crown-5] (III) is achieved by the treatment of HOCH(2)- [15-crown-5] (I) with equimolar amounts of CH(2)CHCH(2)Br (II) in the presence of KOH. The hydrosilylation of III with Si(CH(2)CH(2)CH(2)SiMe(2)H)(4) (IV) in the presence of the Karstedt catalyst affords the crown ether end-c... |
Currently employed bone tissue engineered scaffolds often lack the potential for vascularization, which may be enhanced through the incorporation of and regulated release of angiogenic factors. For this reason, the objective here was to fabricate and characterize protein-loaded amino acid ester polyphosphazene (Pphos)-... |
RNA analysis has been applied to forensic work to determine wound age. We investigated mRNA expression using quantitative RT-PCR of ten genes, including c-fos, fosB, mitogen-activated protein kinase phosphatase-1 (MKP-1), CD14, chemokine (C-C motif) ligand 9 (CCL9), placenta growth factor (PlGF), mast cell protease-5 (... |
We describe in this paper the structure and main features of a domain specific ontology for neuroscience, the BAMS Neuroanatomical Ontology. The ontology includes a complete set of concepts that describe the parts of the rat nervous system, a growing set of concepts that describe neuron populations identified in differ... |
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