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To evaluate spray-freeze drying and spray drying processes for fabricating micron-sized particles of darbepoetin alfa (NESP, Aranesp) with uniform size distribution and retention of protein integrity, requirements for encapsulation. |
Fluid-bed spray-coating process is widely used to prepare non-protein pharmaceutical solid dosage forms using macro-size seed particles (200-1000 microm) at kilogram batch sizes. In this study we developed a small-scale fluid-bed spray-coating process (20 g) to produce micro-sized vaccine powder formulations (40-60 mic... |
A number of neurohormones and cytokines are activated in heart failure and their activity is related to the progression of heart failure. Inhibiting the deleterious effects of the activated sympathetic and the renin-angiotensin-aldosterone systems with b-blockers, angiotensin-converting enzyme inhibitors, and aldostero... |
Left ventricular (LV) remodeling describes dynamic changes in ventricular size and shape that result from hemodynamic and metabolic insults to the failing heart. The remodeling hypothesis in heart failure asserts that LV remodeling is the central pathophysiologic lesion whereby alterations in cardiac structure are foll... |
Results of prospective trials of effects of various antihypertensive drugs on left ventricular hypertrophy in patients with hypertensive disease are reviewed. According to 2 meta-analyses angiotensin converting enzyme inhibitors are most effective inducers of regression of left ventricular hypertrophy. However in compa... |
About 60% of patients with mild and moderate hypertension have insulin resistance and half of them have clinically manifest metabolic syndrome which comprises abdominal obesity, hyperlipidemia, impaired glucose tolerance, hypertension and insulin resistance. In a framework of metabolic syndrome hypertension is characte... |
A library of fluorous, (1H,1H,2H,2H-perfluoroalkyl)silyl-substituted derivatives of triphenylphosphine, Ph(3-a)P[C(6)H(5-y)[SiMe(3-b)(CH(2)CH(2)C(x)F(2x+1))(b)](y)-pos](a) [a = 1-3; b = 1-3; x = 4, 6, 8, or 10; pos = 3, 4 (y = 1) or 3,5 (y = 2)], was prepared using parallel synthetic techniques. Upon variation of these... |
To express Japanese encephalitis virus (JEV) E protein in methylotrophic yeast Pichia pastoris. |
To investigate the killing effect on SKOV3 cells by fusion protein HER2-specific antibody-reversed caspase-3 in the secreted form. |
To prepare and characterize mAb to Fc fragment of Ig fusion protein, and to establish sandwich ELISA for detecting Ig fusion proteins and affinity chromatography method for Ig fusion protein purification. |
To investigate the effect of momordica anti-HIV protein of M(r ) being 30 000 (MAP30) on HBV expression by laser scanning confocal microscopy. |
To study the expression and localization of p27(KIP1)-GFP fusion protein in hepatocellular carcinoma cell line HCC-9204. |
To express fusion protein of GST and vWf binding domain(GP302) of platelet GPIbalpha in E.coli and its preparation of rabbit anti-serum. |
To construct, express and characterize the eukaryotic expression vector of encoding human CD226 (PTA1) extracellular region Ig fusion protein gene containing 3C protease-restricted site. |
To investigate the role of P38 mitogen-activated protein kinase (P38MAPK) in expression of P-selectin on human umbilical vein endothelial cells and diabetic atherosclerosis. |
To assess in a randomized open study effect of 12-15 week use of angiotensin converting enzyme inhibitors (ACEI) with and without trimetazidine on myocardial perfusion reserve in patients with ischemic heart disease (IHD) and/or hypertension associated with type II diabetes. |
To study the activation effect of BCR/ABL antigen on T cells from CML patients mediated by protein transduction domain (PTD). |
To express the fusion protein TGF-betaR II/Fc in large amounts by using recombinant Bac-TR II baculovirus expression system constructed by our laboratory and to purify and characterize it. Then, to verify whether the fusion protein TGF-betaR II/Fc can be able to block the biological activity of cytokine TGF-beta1. |
The C1423T polymorphism in von Willebrand factor-cleaving protease (vWF-cp) gene affects its enzyme activity. The present study was to investigate the polymorphism frequency among Chinese Han population and its relevance to arterial thrombotic disorders. |
To investigate the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cells affected by all-trans retinoic acid (ATRA) and daunorubincin (DNR) respectively. |
During last 10 years the potential interaction between aspirin and angiotensin converting enzyme inhibitors (ACEI) has received considerable attention. Retrospective analysis of randomized trials and their meta-analyses gave conflicting results concerning existence and effects of this interaction. Taking into considera... |
E-MAP-115 (ensconsin) is a microtubule-associated protein (MAP) abundant in carcinoma and other epithelia-derived cells. We expressed chimeras of green fluorescent protein (GFP) conjugated to ensconsin's N-terminal MT-binding domain (EMTB), to study distribution, dynamics, and function of the MAP in living cells. We te... |
In the present study we describe the evaluation of the effectiveness of gonad protection in diagnostic radiology based on the measurement of organ and the effective doses with and without lead clothing to gonads. We devised in-phantom dosimetry system and measured organ and effective doses in x-ray radiography and CT e... |
SPring-8 RIKEN beamline I has been designed and developed for structural biology research by the Institute of Physical and Chemical Research (RIKEN). The beamline consists of two experimental stations for protein crystallography and small-angle X-ray scattering. Both types of experiments can be carried out simultaneous... |
A two-dimensional microstrip gas chamber (MSGC) has been developed with a 10 cm-square detection area and an ultrafast read-out system. The MSGC was made using multi-chip module (MCM) technology, and has a very thin substrate of 17 micro m and many anodes and back strips, both with 200 micro m pitches. The new read-out... |
A multilayer monochromator was installed on a bending-magnet beamline at the Cornell High Energy Synchrotron Source (CHESS) and was used to provide an unfocused pseudo-monochromatic X-ray beam for protein crystallography experiments. Datasets were collected from lysozyme at room temperature and human methylthioadenosin... |
A new multipole wiggler device has been designed for the 2.0 GeV Synchrotron Radiation Source at Daresbury Laboratory in the UK. The nine-pole 2.0 T device will provide radiation for two beamlines dedicated to protein crystallography, one of which will be of high intensity. This article provides details of the design o... |
A novel X-ray beam-position detection device that we call a position-sensitive photoconductive detector (PSPCD) is designed to have synthetic diamond as its substrate material. We proved that it is feasible to use synthetic diamond to make a hard X-ray position-sensitive detector based on the photoconductivity principl... |
The first insertion-device beamline at the Pohang Light Source is designed for high-resolution spectroscopy and spectromicroscopy. The beamline will contain a variable-included-angle plane-grating monochromator (VIA-PGM) using a grating substrate which has seven different grooves with different depths. The advantages o... |
A semitransparent Mo/Si multilayer beam splitter with a completely self-standing active area (10 x 10 mm) and a flatness of 1.1 nm (r.m.s.) was fabricated. The influence of the roughness of the membrane substrate on the reflectivity of a beam splitter was investigated for different materials and deposition schemes. Pre... |
The optical performance of platinum-carbon multilayers deposited onto different substrates has been examined. Specular reflectivity and non-specular diffuse scattering were measured to study the replication of substrate roughness into the multilayer structure. Surface topography was measured before and after deposition... |
A UHV surface X-ray scattering system has been constructed at the SRRC, providing users with a state-of-the-art system for performing X-ray scattering studies of two-dimensional crystallography, in situ growth mechanisms as well as phase transitions of surfaces and interfaces. A study of the phase transition of the Si(... |
As a basic layered structure for giant magnetoresistive (GMR) heads, NiFe/Cu/NiFe/Ta/Si substrate was measured by X-ray reflectometry at Cu Kalpha, Cu Kbeta and Cu K-absorption-edge energies. The accuracy of both the Cu thickness and the interface width between the upper NiFe and the Cu layers was found to improve in t... |
Accurate diffraction intensity data have been collected from a twinned P6(3) crystal of the 24-haem protein hydroxylamine oxidoreductase, from a nitrifying chemoautotrophic bacterium Nitrosomonas europaea, using synchrotron radiation at station BL6A of the Photon Factory. Estimation of the twinning fraction and deconvo... |
To evaluate the application of the conversion-electron-yield (CEY) method in catalyst analysis, the intensities of the CEY and X-ray fluorescence (XRF) as a function of glancing angle were measured simultaneously. The probing depth of the CEY method is shallower than that of the XRF method. The CEY method also shows po... |
Angle-resolved UV photoelectron spectra (ARUPS) were measured for thin films of perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA) deposited on cleaved MoS(2) surfaces. The take-off angle (theta) dependence of the photoelectron intensity of the highest pi band showed a sharp maximum at theta = 32-34 degrees. A spect... |
To investigate the changes in the pulmonary surface tension and the tissue content of surfactant substance protein B (SP-B) in rabbits during early post-injury stage after smoke inhalation injury. |
This study demonstrates utilization of the novel GTG initiation codon for translation of a human mRNA transcript that encodes the serpin endopin 2B, a protease inhibitor. Molecular cloning revealed the nucleotide sequence of the human endopin 2B cDNA. Its deduced primary sequence shows high homology to bovine endopin 2... |
We have determined the Rubisco components and activity, whole leaf protein and amino acid components of thermo-sensitive mutant line 1103s of rice (Oryza sativa ssp. indica) leaves during induces green and yellow banding in this study. The results are as follows: The structure and components of Rubisco in the mutant ar... |
The present study has determined the cellular distribution of cytochrome P450scc in human early placenta by immunohistochemistry and assessed the abundances of cytochrome P450scc protein in the villous tissue at 6-9 weeks of human pregnancy by Western blotting. The results showed that immunoreactive P450scc was mainly ... |
According to results of multiple studies depression and anxiety are found in more than 50% of patients with hypertension. Presence of affective disorders elevates risk of progression of hypertension. That is why complex therapy comprising antihypertensive drugs from various groups and antidepressants takes on higher an... |
To elucidate effect of beta-blocker bisoprolol on hibernating myocardium in patients with congestive heart failure (CHF) of ischaemic etiology without concomitant use of angiotensin converting enzyme inhibitors. |
In order to shed light on the mechanisms of TGF-beta action in the testis,we examined the expression and function of Smad4 protein, the common-mediator Smads, which is one of intracellular signaling molecules of TGF-beta superfamily members, in rat testis during postnatal development. Whole testes were collected from S... |
The localization of the new estrogen receptor, ER-beta, in the rat brain was studied by immunocytochemical technique, and the results revealed that ER-beta immunoactive material was predominantly localized in the neuronal nucleus, but it was also detectable in the cytoplasm and neuronal processes. High levels of ER-bet... |
The centrosomal protein, Cenexin, is a molecular marker of mature centriole. To elucidate the variability and function of mature centriole in spermatogenesis, the high titer polyclonal antibody against rat cenexin was obtained by immunizing mice with recombinant cenexin which was made up in this study. The expression o... |
Differentially expressed genes between normal liver and hepatocellular carcinomas were investigated using differential display. In previous study, human F-LANa was identified as a differentially expressed gene, up-regulated in hepatocellular carcinoma. In this study, we developed an in silico cloning approach to rapidl... |
The most commonly observed change that has been linked to resistance development is the increase in activity of carboxylesterases. The putative mechanism involves an overproduction of this enzyme for the sequestration and the hydroxylation of various organophosphate and carbamate insecticides. Carboxylesterases A2 cDNA... |
In order to explore the role of MNSFbeta in the process of implantaion, MNSFbeta and its antibodies are required. The expression plasmid pBV220/MNSFbeta-hCGbeta was constructed, and then transferred into E. coli to express the fusion protein MNSFbeta-hCGbeta. The anti-hCGbeta antibody was used to identify the fusion pr... |
In this study, the possibility of sheep transgenesis by intracytoplasmic sperm injection (ICSI) was assessed. In experiment 1, activation of ovine oocytes matured in vitro in preparation for ICSI has been investigated with 3.42 mmol/L Ca2+ treatment, ionomycin alone and ionomycin followed by 6-dimethylaminopurine (DMAP... |
Bt-CpTI fusion protein gene was transferred to the explants of hypocotyl, cotyledon with petiole and shoot apex of cabbage (Brassica oleracea L. var. capitata) variety "Zhonggan No 8" via Agrobacterium tumefaciens and particle bombardment, and 13 kanamycin-resistant plants were obtained. PCR and Southern blotting hybri... |
The term RNA editing is generally used to describe those molecular processes in which the information content is altered in an RNA molecule. This process is not limited to mRNA since alterations of non-informational RNA have also been found. RNA editing exists extensively in the higher plant mitochondria, and is the ne... |
The mouse zinc finger protein ZF-12 gene is homologous to human gene and encodes a protein of 368 amino acids, which contains four tandem C2H2-type zinc finger motifs in the N-terminal and one SCAN domain in the C-terminal. Some recent studies suggest that ZNF191 might be a hepatocarcinogenesis-associated gene. We scre... |
Fluo-3/AM, a calcium indicator, was introduced by low temprature loading method into callus protoplasts of Stevia rebaudiana Bertoni. The microscope observation results showed that NaCl in different concentrations (30-200mmol/L) can elevate the intracellular calcium concentration in protoplasts. The elevation was relat... |
The bcl-2 protein, which widely expressed in the developing central and peripheral nervous systems, has the functional role of blocking apoptosis. The purpose of this study was to map bcl-2 expression in the human enteric nervous system and investigate the value of bcl-2 immunohistochemical method in the diagnosis of H... |
The study on proteome of human cancer is helpful to explain its pathogenesis and make good effect on its prevention and cure. We compared the 2-DE maps of whole proteins of human lung cancer line A549 at 37 degrees C, 42 degrees C and 45 degrees C for the purpose of studying the expression of its heat shock proteins. T... |
The phosphorylation of the fibroblast growth factor receptor (FGFR) kinase substrate SNT1 (also called FGFR substrate 2, FRS2) by FGFR tyrosine kinases is both host cell- and receptor isotype-specific. To study the determinants of the host cell-specific phosphorylation of SNT1 by FGFR1 tyrosine kinase, we constructed a... |
Pot experiment was used to study the responses of membrane protection enzyme system of tobacco leaves on Hg, Cd and Pb stresses in soil. The results showed that POD activity gradually increased with increasing concetrations of Hg, Cd and Pb. CAT and SOD activity gradually decreased under three heavy metals common exist... |
Chalcone synthase (CHS) is a key enzyme in the biosynthesis of all classes of flavonoids. The production of flower pigment is specifically regulated by the activity of CHS. We cloned the cDNA sequence of CHS-A gene from Narcissus by PCR and analyzed the coding sequence of gene. The result demonstrated that the sequence... |
Increases of functional T-type calcium channel (T-channel) expression have been associated with cellular proliferation although evidence for this remains controversial. In the present study, we have used a variety of cellular, molecular and electrophysiological techniques to test the hypothesis that T-type channels pla... |
Abscisic acid (ABA) was critical in plant seed development and response to environmental factors such as stress situations. To study the possible ABA related signaling transduction pathways, we tried to isolate the ABA-regulated genes through fluorescent differential display PCR (FDD-PCR) technology using rice seedling... |
Silver crucian carp (Carassius auratus gibelio) has been known for its gynogenetic reproduction. In this paper, eggs from clone F of gynogenetic silver crucian carp were inseminated respectively by sperms of clone D, clone A and common carp, and polymorphic patterns of transferrin and isozymes of the produced three off... |
The ultrastructures of storage protein accumulation in cotyledons and aleurone layer in buckwheat (Fagopyrum esculentum Monch) were investigated by electron microscopy. (1) On 15 days after anthesis (DAA), there are many dictyosomes and vacuoles accumulating protein in cytoplasm of the outer layer endosperm cells. Thes... |
Congenital lipoid adrenal hyperplasia (CLAH) is an autosomal recessive inherited disorder, characterized by deficiency of adrenal and gonadal steroid hormones. Recent studies have shown that mutations in the gene for steroidogenic acute regulatory protein (StAR) cause this most severe genetic disorder in steroid hormon... |
To investigate the relationship of Fas mRNA and protein expression and apoptosis in human oral squamous cell carcinoma. |
While studies of schistosome antigens have proceeded rapidly over the past ten years, studies of schistosome enzymes have also been increasing apace. Now the two `lines' of research are coming together. Parasites such as schistosomes hardly present antigens merely to stimulate a host immune response, so it is not surpr... |
Neonatal septicemia is a critical disease in neonatal period. Its incidence among live births is between 1 per thousand and 8 per thousand. Mortality of neonatal septicemia may be as high as 50% for infants who are not treated. The early signs of septicemia in the newborn are generally nonspecific. Blood culture and th... |
To enhance the immunogenicity of hCGbeta protein vaccine and the hCG neutralization capacity of anti-serum by using the molecular adjuvant C3d3, the secreted 6his-hCGbeta-C3d3 fusion protein and 6his-hCGbeta were expressed in CHO cells and purified with Ni(2+)-chelating chromatography and Sephadex G-150 column. Then we... |
A full length cDNA clone encoding invertase inhibitor was isolated by RT-PCR combined with 5' RACE from potato (S. tuberosum) tubers of cv. JH and designated as St-inh. The encoding region of St-inh is of 663bp encoding a protein of 221 amino acids. The DNA fragment including St-inh cDNA was cloned into the vector pET2... |
To study the pathogenesis of dysosphresia in patients with chronic sinusitis, to discuss the expression and significance of olfaction marker protein in olfactory mucosa. |
Two genes (MAT1A and MAT2A) encode for the essential enzyme methionine adenosyltransferase (MAT). MAT1A is silenced in hepatocellular carcinoma (HCC), and absence of MAT1A leads to spontaneous development of HCC in mice. Here we investigated the role of methylation in regulating MAT1A expression. There are three MspI/H... |
To elucidate relationships between blood levels of matric metalloproteinases (MMP), C-reactive protein (CRP), markers of thrombinemia, and development of restenosis after percutaneous coronary interventions (PCI) in patients with stable angina. |
To investigate the expression and distribution of extracellular-regulated protein kinase (ERK) and c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) under the mechanical pressure in rabbit mandibular condylar chondrocytes (MCC). |
The surface of oral mucous is highly specialized stratified squamous epithelium that plays an important role in protecting the tissue beneath it from physical, chemical or biological damages. However, the change of the epithelium structure as well as the related deficiency in functions due to some genetic or immunologi... |
In order to illustrate the possible roles of PML-RARalpha protein in arsenic trioxide (AsO3)-induced NB4 cell apoptosis. |
To detect the expression of glycosyl-phosphatidyl-inositol (GPI) anchored protein on the blood cell membrane and its implication in the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). |
To investigate the Factor V Leiden mutation associated with activated protein C resistance (APCR) in Chinese. |
To explore the regulatory mechanism of iron responsive element binding protein (IRE-BP) in iron metabolism. |
Human Heme Oxygenase-1 (hHO-1) is the rate-limiting enzyme in the catabolism reaction of heme, which directly regulates the concentration of bilirubin in human body. The mutant structure was simulated by Swiss-pdbviewer procedure, which showed that the structure of active pocket was changed distinctly after Ala25 subst... |
To study the oncogene transcriptor c-myc, stimulatory protein 1 (SP1) expression in ameloblastoma (AB) and their relation with telomerase reverse transcripase (hTERT), and to investigate the clinical biological characteristics of AB. |
Dilated cardiomyopathy (DCM) is characterized by enlargement and dilation of all heart compartments associated with serious decrease of its contractile function. DCM hallmark is the combination of dystrophic and hypertrophic alterations of cardiomyocytes. Since the power output of cardiac cells is directly related to r... |
In part V of a series of papers on epidemiology and drug prevention of stroke and other thromboembolic complications of atrial fibrillation the authors analyze data of randomized trials exhibiting ability of long term use of beta-adrenoblockers, angiotensin converting enzyme inhibitors, angiotensin receptor antagonists... |
A series of hyperbranched poly(amine-ester)s were synthesized using one-step and pseudo-step procedure respectively, and their molecular structures were characterized by IR, hydroxyl value and viscosity. These polymers were coated on the inner surface of the fused-silica capillaries in a chemical bonding process. The e... |
Hyperphosphorylated microtubule-associated protein tau is the major protein component of neurofibrillary tangles in the brain of patients with Alzheimer's disease (AD). Until now, there is no effective cure to arrest this hyperphosphorylation. The present study was designed to explore the in vivo preventive effect of m... |
Increased vascular endothelial growth factor (VEGF) biosynthesis in vascular endothelial cells has been reported to play an obligatory role in promoting angiogenesis. Nevertheless, the intracellular signaling mechanisms of hypoxia-induced VEGF release remain largely unknown. Human umbilical vein endothelial cell lines ... |
A reversed-phase HPLC assay has been developed for measuring cefotaxime (CTX) in human serum. A solid-phase extraction column (Bond Elute C18) was used for the pre-purification of sample. Serum (200 microL) was mixed with 100 microL of internal standard (acetylamino phenol) solution and 200 microL of sodium acetate buf... |
A rapid high performance liquid chromatography for determination of demethylvancomycin in neonate serum has been developed. The procedure involved a simple protein precipitation by acetonitrile-isopropanol (1:1) and then the sample was chromatographed on a reversed phase C18 column with UV detection at 236 nm. The mobi... |
Based on inorganic matrix controlled pore glass (CPG) and macro-pore silica sphere, by using polyethylene glycol (PEG 1000) as a ligand, a preparation method of hydrophobic interaction chromatographic (HIC) packing material was improved by adding a proper catalyst during the bonding process. The packing material can be... |
Hydrophobic interaction chromatography (HIC) is an attractive method for protein purification. The paper gives a review on the characteristics of HIC packings, including matrices and hydrophobic ligands. The efficiencies for protein separations were compared for different HIC packings. The present situation and the dev... |
L-4-oxalysinyl-norvalinyl-N3-4-methoxyfumaroyl-L-2,3-diaminopropanoic acid (I-677-Nva-FMDP) is a new tripeptide synthesized in our group. This peptide exhibits potent anticandida Albicans activity. In the presence of serum, the antifun gal activity of I-677-Nva-FMDP decreases after incubating over 1h. In order to inves... |
From the seeds of Pachyrrhizus errosus, three protein constituents, namel PE1, PE2 and PE3, have been isolated and purified by extraction with 5mmol/L phosphate saline (0.9% NaCl) buffer (PB) at pH 7.2, and S-Sepharose Fast Flow Column (2.6cm x 15cm) chromatography which eluted with 5mmol/L phosphate buffer (pH 7.0) co... |
A new type of SnO2/SnCl2 semiconductor as detector in gas chromatography has been developed. The sensing crystalloid with SnO2 as main block has an outstanding sensitivity to the reducing gases in a certain oxidative circumstance. The reducing gases can be reversibly adsorbed on the surface of crystal and make the resi... |
A capillary isoelectric focusing (CIEF) method was developed for the separation of proteins with different pI. Uncoated capillary (57 cm x 50 microm i.d.) was used instead of commercial coated column because of its ease in use and a longer lifetime. Polymeric additive, methylcellulose (MC-1500), acts as dynamic coating... |
High-performance ion exchange chromatography (HPIEC) is extensively used in the separation of peptides and proteins, especially in the biotechnology process. The principle of separation of proteins is based on the changes of pH and salt concentration in the mobile phase for the chromatographic model. A new synthetic me... |
A high performance liquid chromatographic system is described for the purification to homogeneity of recombinant human gamma-interferon (gamma-IFN) from the inclusion bodies produced in genetically transformed Escherichia Coli cells. Crude products of gamma-IFN obtained from typical preparative liquid chromatographic s... |
Analysis of butyraldehyde obtained by the interaction of butanol with cytochrome P450 II E1 in rat liver microsomes prepared by centrifugation and with the NADPH added is described in this paper. The biotransformation rate of butanol into butyraldehyde can be used as an index for the assessment of the enzyme activity o... |
A series of unexpected products were obtained when 1,4-dichloro-2-butyne reacted with allyl bromide with the catalyst PdCl2 (PhCN)2. It is difficult to separate these products from each other and to identify the structure of each one due to their similar properties. In order to solve this problem, gas chromatography-ma... |
Catalytic hydrogenation of nitrobenzene with supported palladium catalyst is a new method to produce p-aminophenol. p-Aminophenol, aniline and 4,4'-diaminodiphenyl ether obtained from this method were determined by reversed phase high performance liquid chromatography. The factors, e.g., concentration of methanol, pH a... |
An RP-HPLC method is described for the assay of propofol (2,6-diisopropylphenol), a new anesthesia drug, in human plasma. Protein in sample was precipitated with methanol, followed by extraction of propofol with cyclohexane. Both propofol and the internal standard thymol were derivatized with Gibbs' reagent and then ch... |
The hydrolytic solution of proteins mainly contains free amino acids and peptides. The separation of amino acids and di- and tri-peptides is very significant and also a complicated work. This report presents a chelated metal ion affinity chromatographic (CMAC) method for the separation of alpha-amino acids and peptides... |
The levels of R- and S-mephenytoin in human urine were determined by chiral capillary gas chromatography with nitrogen-phosphorus detector. The conditions of the chromatography and detection included a chiral capillary column (Chirasil-Val, 25 m x 0.25 mm i.d., Alltech), column temperature (T) 190 degrees C, injector T... |
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