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1,284 | https://bio-protocol.org/exchange/protocoldetail?id=1284&type=0 | # Bio-Protocol Content
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Measurement of the Number of Peroxisomes
MS Michitaro Shibata
Kazusato Oikawa
SM Shoji Mano
Mikio Nishimura
Published: Vol 4, Iss 21, Nov 5, 2014
DOI: 10.21769/BioProtoc.1284 Views: 9811
Edited by: Ru Zhang
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1,285 | https://bio-protocol.org/exchange/protocoldetail?id=1285&type=0 | # Bio-Protocol Content
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In vitro Assay of the Glycosyltransferase Activity of a Heterologously Expressed Plant Protein
CA Carsten P Ade
FB Felix Bemm
JD James M J Dickson
CW Christian Walter
PH Philip J Harris
Published: Vol 4, Iss 21, Nov 5, 2014
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1,286 | https://bio-protocol.org/exchange/protocoldetail?id=1286&type=0 | # Bio-Protocol Content
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Stable Transformation of Cyanobacterium Synechocystis sp.
Reinhard K Proels
Published: Vol 4, Iss 21, Nov 5, 2014
DOI: 10.21769/BioProtoc.1286 Views: 13286
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Fractionation by Ultracentrifugation of Gram Negative Cytoplasmic and Membrane Proteins
SS Sara M Sandrini
RH Richard Haigh
PF Primrose P. E. Freestone
Published: Vol 4, Iss 21, Nov 5, 2014
DOI: 10.21769/BioProtoc.1287 Views:... |
1,288 | https://bio-protocol.org/exchange/protocoldetail?id=1288&type=0 | # Bio-Protocol Content
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Pain Assessment Using the Rat and Mouse Formalin Tests
Nian Gong
Qian Huang
Yuan Chen
Meng Xu
Shuai Ma
Yong-Xiang Wang
Published: Vol 4, Iss 21, Nov 5, 2014
DOI: 10.21769/BioProtoc.1288 Views: 26434
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1,289 | https://bio-protocol.org/exchange/protocoldetail?id=1289&type=0 | # Bio-Protocol Content
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Analysis of Intestinal Permeability in Mice
Jalaj Gupta
AN Angel R. Nebreda
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1289 Views: 45646
Reviewed by: Kate HannanIsabel Cristiane da Silva
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129 | https://bio-protocol.org/exchange/protocoldetail?id=129&type=1 | # Bio-Protocol Content
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Expansion of Worms for Microarray, IP, ChIP and Similar Experiments
Peichuan Zhang
Published: Sep 5, 2011
DOI: 10.21769/BioProtoc.129 Views: 11601
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1,290 | https://bio-protocol.org/exchange/protocoldetail?id=1290&type=0 | # Bio-Protocol Content
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Induction of Colitis and Colitis-associated Colorectal Cancer (CAC)
Jalaj Gupta
AN Angel R. Nebreda
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1290 Views: 15620
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1,291 | https://bio-protocol.org/exchange/protocoldetail?id=1291&type=0 | # Bio-Protocol Content
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Preparation of Golgi Membranes from Rat Liver
CV Carmen Valente
GT Gabriele Turacchio
SS Stefania Spanò
AL Alberto Luini
DC Daniela Corda
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1291 Views: 8636
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1,292 | https://bio-protocol.org/exchange/protocoldetail?id=1292&type=0 | # Bio-Protocol Content
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Isolation of FAP Cells from Mouse Dystrophic Skeletal Muscle Using Fluorescence Activated Cell Sorting
Nicoletta Cordani
VP Viviana Pisa
LP Laura Pozzi
Clara Sciorati
Published: Vol 4, Iss 22, Nov 20, 2014
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1,293 | https://bio-protocol.org/exchange/protocoldetail?id=1293&type=0 | # Bio-Protocol Content
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Purification and Sequencing of DNA Guides from Prokaryotic Argonaute
Daan C. Swarts
EW Edze R. Westra
Stan J. J. Brouns
John van der Oost
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1293 Views: 13929
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Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)
DL Damya Laoui
EO Eva Van Overmeire
JK Jiri Keirsse
KM Kiavash Movahedi
JG Jo A Van Ginderachter
Published: Vol 4, Iss 22, Nov 20,... |
1,295 | https://bio-protocol.org/exchange/protocoldetail?id=1295&type=0 | # Bio-Protocol Content
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Infectious Focus Assays and Multiplicity of Infection (MOI) Calculations for Alpha-herpesviruses
Anna Sloutskin
RG Ronald S. Goldstein
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1295 Views: 43015
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1,296 | https://bio-protocol.org/exchange/protocoldetail?id=1296&type=0 | # Bio-Protocol Content
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In vitro Detection of S-acylation on Recombinant Proteins via the Biotin-Switch Technique
DQ Dong Qi
Roger W. Innes
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1296 Views: 9270
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1,297 | https://bio-protocol.org/exchange/protocoldetail?id=1297&type=0 | # Bio-Protocol Content
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Semi-denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE)
Laura Molina-García
Fátima Gasset-Rosa
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1297 Views: 22686
Edited by: Fanglian He
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1,298 | https://bio-protocol.org/exchange/protocoldetail?id=1298&type=0 | # Bio-Protocol Content
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Competitive ELISA for Protein-lipopolysaccharide (LPS) Binding
Victoria Martínez-Sernández
Florencio M. Ubeira
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1298 Views: 14251
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1,299 | https://bio-protocol.org/exchange/protocoldetail?id=1299&type=0 | # Bio-Protocol Content
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Separation of the Inner and Outer Mitochondrial Membrane in HeLa Cells
NN Naotaka Nishimura
MY Masato Yano
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1299 Views: 27819
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Metabolic Labeling of Yeast Proteins
Bio-protocol Editor
Published: Jan 5, 2011
DOI: 10.21769/BioProtoc.13 Views: 13424
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Proteins of Saccharomyces cerevisiae can be metabo... |
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Stable Caco-2 Cell Line Construction
Lin Fang
Published: Vol 2, Iss 6, Mar 20, 2012
DOI: 10.21769/BioProtoc.130 Views: 21294
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1,300 | https://bio-protocol.org/exchange/protocoldetail?id=1300&type=0 | # Bio-Protocol Content
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Isolation and Characterisation of Dendritic Cells from Peripheral Blood
MR Matthew Reeves
JS John Sinclair
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1300 Views: 16738
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1,301 | https://bio-protocol.org/exchange/protocoldetail?id=1301&type=0 | # Bio-Protocol Content
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Assay of Ornithine Decarboxylase and Spermidine/Spermine N1-acetyltransferase Activities
Mervi T. Hyvönen
TK Tuomo Keinänen
Leena Alhonen
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1301 Views: 9598
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1,302 | https://bio-protocol.org/exchange/protocoldetail?id=1302&type=0 | # Bio-Protocol Content
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Secretion Assay in Shigella flexneri
JR Jonathan Reinhardt
MK Michael Kolbe
Published: Vol 4, Iss 22, Nov 20, 2014
DOI: 10.21769/BioProtoc.1302 Views: 10800
Edited by: Fanglian He
Reviewed by: Kanika Gera
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1,303 | https://bio-protocol.org/exchange/protocoldetail?id=1303&type=0 | # Bio-Protocol Content
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Detection of ALT Associated Promyelocytic Leukemia Nuclear Bodies (APBs) by Immunofluorescence-FISH (IF-FISH)
Siamak A. Kamranvar
Maria G. Masucci
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1303 Views: 1314... |
1,304 | https://bio-protocol.org/exchange/protocoldetail?id=1304&type=0 | # Bio-Protocol Content
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Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons
HL Hongmei Li
HP Han-A Park
Elizabeth A. Jonas
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1304 Views: 11159... |
1,305 | https://bio-protocol.org/exchange/protocoldetail?id=1305&type=0 | # Bio-Protocol Content
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Visualization of Cell Complexity in the Filamentous Cyanobacterium Mastigocladus laminosus by Transmission Electron Microscopy (TEM)
Dennis J. Nürnberg
GM Giulia Mastroianni
CM Conrad W. Mullineaux
GM Graham D. McPhail
Publis... |
1,306 | https://bio-protocol.org/exchange/protocoldetail?id=1306&type=0 | # Bio-Protocol Content
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In situ Digestion of Wheat Cell-wall Polysaccharides
DV Dušan Veličković
HR Hélène Rogniaux
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1306 Views: 11239
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T... |
1,307 | https://bio-protocol.org/exchange/protocoldetail?id=1307&type=0 | # Bio-Protocol Content
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Induction of Tigecycline Resistance in Acinetobacter baumannii
Ming-Feng Lin
YL Yun-You Lin
Chung-Yu Lan
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1307 Views: 8285
Edited by: Arsalan Daudi
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1,308 | https://bio-protocol.org/exchange/protocoldetail?id=1308&type=0 | # Bio-Protocol Content
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Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii
Ming-Feng Lin
YL Yun-You Lin
Chung-Yu Lan
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1308 Views: 47394
Edited by: Arsalan Daudi
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1,309 | https://bio-protocol.org/exchange/protocoldetail?id=1309&type=0 | # Bio-Protocol Content
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Vanillin Assay of Arabidopsis Seeds for Proanthocyanidins
LX Lijie Xuan
ZW Zhong Wang
Lixi Jiang
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1309 Views: 11557
Edited by: Tie Liu
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Th... |
131 | https://bio-protocol.org/exchange/protocoldetail?id=131&type=1 | # Bio-Protocol Content
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Chloroform-ethanol Isolation Genomic DNA from Mouse Tail
Lin Fang
Published: Sep 20, 2011
DOI: 10.21769/BioProtoc.131 Views: 11809
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1,310 | https://bio-protocol.org/exchange/protocoldetail?id=1310&type=0 | # Bio-Protocol Content
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CytoTrap Two-Hybrid Screening Assay
LZ Lei Zhang
LD Liqun Du
B. W. Poovaiah
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1310 Views: 12430
Edited by: Tie Liu
Reviewed by: Saminathan Thangasamy
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1,311 | https://bio-protocol.org/exchange/protocoldetail?id=1311&type=0 | # Bio-Protocol Content
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Infection Assays of Tomato and Apple Fruit by the Fungal Pathogen Botrytis cinerea
ZZ Zhanquan Zhang
GQ Guozheng Qin
BL Boqiang Li
Shiping Tian
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1311 Views: 12700
E... |
1,312 | https://bio-protocol.org/exchange/protocoldetail?id=1312&type=0 | # Bio-Protocol Content
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Enzymatic Activity Assays in Yeast Cell Extracts
Melike Çağlayan
SW Samuel H. Wilson
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1312 Views: 10962
Edited by: Fanglian He
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1,313 | https://bio-protocol.org/exchange/protocoldetail?id=1313&type=0 | # Bio-Protocol Content
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TGFβ Stimulation Assay
Emanuel Rognoni
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1313 Views: 14792
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The authors used this protocol in Apr 2014
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1,314 | https://bio-protocol.org/exchange/protocoldetail?id=1314&type=0 | # Bio-Protocol Content
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TGFβ Release Co-culture Assay
Emanuel Rognoni
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1314 Views: 11004
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1,315 | https://bio-protocol.org/exchange/protocoldetail?id=1315&type=0 | # Bio-Protocol Content
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Creating a Rat Model of Chronic Variate Stress
Rocío M. de Pablos
Manuel Sarmiento
Ana María Espinosa-Oliva
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.21769/BioProtoc.1315 Views: 11149
Edited by: Soyun Kim
Reviewed by: Emm... |
1,316 | https://bio-protocol.org/exchange/protocoldetail?id=1316&type=0 | # Bio-Protocol Content
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Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
TA Tomás Albi
MO M. Isabel Ortiz-Marchena
MR M. Teresa Ruiz
José M. Romero
Federico Valverde
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1,317 | https://bio-protocol.org/exchange/protocoldetail?id=1317&type=0 | # Bio-Protocol Content
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Determination of Soluble Sugars in Arabidopsis thaliana Leaves by Anion Exchange Chromatography
MO M. Isabel Ortiz-Marchena
MR M. Teresa Ruiz
Federico Valverde
José M. Romero
Published: Vol 4, Iss 23, Dec 5, 2014
DOI: 10.2176... |
132 | https://bio-protocol.org/exchange/protocoldetail?id=132&type=1 | # Bio-Protocol Content
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Expression and Purification of GST-tagged Proteins from E. coli
Lin Fang
Published: Sep 20, 2011
DOI: 10.21769/BioProtoc.132 Views: 21659
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133 | https://bio-protocol.org/exchange/protocoldetail?id=133&type=0 | # Bio-Protocol Content
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Antibody Purification from Western Blotting
Lin Fang
Published: Vol 2, Iss 6, Mar 20, 2012
DOI: 10.21769/BioProtoc.133 Views: 22678
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134 | https://bio-protocol.org/exchange/protocoldetail?id=134&type=1 | # Bio-Protocol Content
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Yeast in situ Hybridization
Wei Zheng
Published: Sep 20, 2011
DOI: 10.21769/BioProtoc.134 Views: 11711
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This protocol describes the procedure to label a certain mRNA speci... |
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qPCR of Yeast ChIP DNA
Wei Zheng
Published: Sep 20, 2011
DOI: 10.21769/BioProtoc.135 Views: 17103
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This protocol is adapted from Chris Yellman's personal notes for qPCR of... |
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Cyclohexane Diamine Tetraacetic Acid (CDTA) Extraction of Plant Cell Wall Pectin
GB Gerit Bethke
JG Jane Glazebrook
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1357 Views: 11724
Edited by: Renate Weizbauer
... |
1,358 | https://bio-protocol.org/exchange/protocoldetail?id=1358&type=0 | # Bio-Protocol Content
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RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
Germano Cecere
Alla Grishok
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1358 Views: 14362
Edited by: Fanglian He
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Virus-induced Gene Silencing (VIGS) in Phalaenopsis Orchids
Ming-Hsien Hsieh
ZP Zhao-Jun Pan
Hsin-Hung Yeh
Hong-Hwa Chen
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1359 Views: 12732
Edited by: Tie Liu
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136 | https://bio-protocol.org/exchange/protocoldetail?id=136&type=1 | # Bio-Protocol Content
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Preparation of Taq DNA Polymerase
Wei Zheng
Published: Oct 5, 2011
DOI: 10.21769/BioProtoc.136 Views: 34363
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Dialysis tubing cellulose membrane avg. flat wid... |
1,360 | https://bio-protocol.org/exchange/protocoldetail?id=1360&type=0 | # Bio-Protocol Content
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Imaging and Measurement of Nanomechanical Properties within Primary Xylem Cell Walls of Broadleaves
Jaroslav Ďurkovič
MK Monika Kardošová
RL Rastislav Lagaňa
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1360... |
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Trichome Isolation and Integrity Test from Brassica villosa and Other Species
NN Naghabushana Nayidu
PB Peta Bonham-Smith
MG Margaret Y. Gruber
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1361 Views: 9526
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1,362 | https://bio-protocol.org/exchange/protocoldetail?id=1362&type=0 | # Bio-Protocol Content
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Agrobacterium-mediated Transformation of Mature Ginseng Embryos
Ok Ran Lee
JH Jin Haeng Han
YK Yoon-Ri Kim
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1362 Views: 11355
Edited by: Tie Liu
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Localization and Topology of Thylakoid Membrane Proteins in Land Plants
ST Salar Torabi
MP Magdalena Plöchinger
JM Jörg Meurer
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1363 Views: 12381
Edited by: Ru Zha... |
1,364 | https://bio-protocol.org/exchange/protocoldetail?id=1364&type=0 | # Bio-Protocol Content
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Polysomal-mRNA Extraction from Arabidopsis by Sucrose-gradient Separation
SL Shih-Yun Lin
Guang-Yuh Jauh
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1364 Views: 11694
Edited by: Tie Liu
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Hepatitis C virus Cell-to-cell Spread Assay
Naina Barretto
Susan L. Uprichard
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProtoc.1365 Views: 10632
Reviewed by: Lee-Hwa Tai
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The authors us... |
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Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24Gag
MG Marianne Gesner
MM Mekhala Maiti
RG Robert Grant
Marielle Cavrois
Published: Vol 4, Iss 24, Dec 20, 2014
DOI: 10.21769/BioProt... |
1,367 | https://bio-protocol.org/exchange/protocoldetail?id=1367&type=0 | # Bio-Protocol Content
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Collection of Root Exudate from Duckweed
Yufang Lu
LS Li Sun
Weiming Shi
Published: Vol 5, Iss 1, Jan 5, 2015
DOI: 10.21769/BioProtoc.1367 Views: 13647
Edited by: Arsalan Daudi
Reviewed by: Samik BhattacharyaTie Liu
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Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
Alexia Daoust
Yasmina Saoudi
JB Jacques Brocard
NC Nora Collomb
Cécile Batandier
MB Mariano Bisbal
MS Murielle Salomé
AA Annie Andrieux
SB Sylvain Bohic
EB Emm... |
1,369 | https://bio-protocol.org/exchange/protocoldetail?id=1369&type=0 | # Bio-Protocol Content
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Imaging and Quantitative Analysis of Size and Distribution of Spherical Bodies, e.g. Embryonic Oil Bodies
Martine Miquel
GT Ghassen Trigui
AT Alain Trubuil
Bertrand Dubreucq
Published: Vol 5, Iss 1, Jan 5, 2015
DOI: 10.21769/... |
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Intracellular Cytokine Staining on PBMCs Using CyTOFTM Mass Cytometry
DL Dongxia Lin
SG Sheena Gupta
Holden Maecker
Published: Vol 5, Iss 1, Jan 5, 2015
DOI: 10.21769/BioProtoc.1370 Views: 22704
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In vitro Migration Assays for Neural Stem Cells, Intermediate Neurogenic Progenitors and Immature Neurons
JL Julia Ladewig
PK Philipp Koch
Oliver Brüstle
Published: Vol 5, Iss 1, Jan 5, 2015
DOI: 10.21769/BioProtoc.1371 Views... |
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Quantification of Extracellular Ammonium Concentrations and Transporter Activity in Yeast Using AmTrac Fluorescent Sensors
Cindy Ast
WF Wolf B. Frommer
GG Guido Grossmann
Roberto De Michele
Published: Vol 5, Iss 1, Jan 5, 201... |
1,373 | https://bio-protocol.org/exchange/protocoldetail?id=1373&type=0 | # Bio-Protocol Content
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Three Dimensional Spheroid Co-culture Invasion Assay
EV Eldo T Verghese
TH Thomas A Hughes
Published: Vol 5, Iss 1, Jan 5, 2015
DOI: 10.21769/BioProtoc.1373 Views: 12625
Reviewed by: Lin Fang
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1,374 | https://bio-protocol.org/exchange/protocoldetail?id=1374&type=0 | # Bio-Protocol Content
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Analysis of Protein Stability by the Cycloheximide Chase Assay
SK Shih-Han Kao
WW Wen-Lung Wang
CC Chi-Yuan Chen
YC Yih-Leong Chang
YW Yi-Ying Wu
YW Yi-Ting Wang
SW Shu-Ping Wang
AN Alexey I Nesvizhskii
YC Yu-Ju Chen
TH Tse-M... |
1,375 | https://bio-protocol.org/exchange/protocoldetail?id=1375&type=0 | # Bio-Protocol Content
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Lipid Extraction from Mouse Feces
Daniel Kraus
QY Qin Yang
BK Barbara B. Kahn
Published: Vol 5, Iss 1, Jan 5, 2015
DOI: 10.21769/BioProtoc.1375 Views: 17328
Reviewed by: Peichuan Zhang
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1,376 | https://bio-protocol.org/exchange/protocoldetail?id=1376&type=0 | # Bio-Protocol Content
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Construction of FWPV Chimaeric MVA
KB Karen R. Buttigieg
Michael A. Skinner
Published: Vol 5, Iss 1, Jan 5, 2015
DOI: 10.21769/BioProtoc.1376 Views: 8660
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1,377 | https://bio-protocol.org/exchange/protocoldetail?id=1377&type=0 | # Bio-Protocol Content
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Determination of Mitochondrial DNA Upon Drug Treatment
MP Michel Perron
Joy Y. Feng
Published: Vol 5, Iss 2, Jan 20, 2015
DOI: 10.21769/BioProtoc.1377 Views: 8213
Reviewed by: Vanesa Olivares-IllanaPia Giovannelli
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Mitochondrial Biogenesis Assay after 5-day Treatment in PC-3 Cells
Yili Xu
Joy Y. Feng
Published: Vol 5, Iss 2, Jan 20, 2015
DOI: 10.21769/BioProtoc.1378 Views: 11285
Reviewed by: Vanesa Olivares-IllanaPia Giovannelli
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1,379 | https://bio-protocol.org/exchange/protocoldetail?id=1379&type=0 | # Bio-Protocol Content
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Camalexin Quantification in Arabidopsis thaliana Leaves Infected with Botrytis cinerea
Daniel V. Savatin
NB Nora Gigli Bisceglia
MG Matteo Gravino
CF Claudia Fabbri
DP Daniela Pontiggia
Benedetta Mattei
Published: Vol 5, Iss ... |
138 | https://bio-protocol.org/exchange/protocoldetail?id=138&type=1 | # Bio-Protocol Content
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Phenol-chloroform Based RNA Extraction from Yeast
Wei Zheng
Published: Oct 5, 2011
DOI: 10.21769/BioProtoc.138 Views: 14237
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DEPC treated water
Phenol (TE)/C... |
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Loading of Cells with Fluorescent Probe to Study Intracellular Acid-base Homeostasis in Lactic Acid Bacteria
PM Pablo Mortera
FZ Federico Zuljan
CM Christian Magni
SA Sergio H. Alarcón
Published: Vol 5, Iss 2, Jan 20, 2015
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In vitro Dynamic Model of a Catheterized Bladder and Biofilm Assay
MM Mario Maierl
MJ Michael Jörger
PR Patrik Rosker
Andreas Reisner
Published: Vol 5, Iss 2, Jan 20, 2015
DOI: 10.21769/BioProtoc.1381 Views: 12508
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1,382 | https://bio-protocol.org/exchange/protocoldetail?id=1382&type=0 | # Bio-Protocol Content
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Phenotyping of Live Human PBMC using CyTOFTM Mass Cytometry
ML Michael D. Leipold
Holden Maecker
Published: Vol 5, Iss 2, Jan 20, 2015
DOI: 10.21769/BioProtoc.1382 Views: 19134
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1,383 | https://bio-protocol.org/exchange/protocoldetail?id=1383&type=0 | # Bio-Protocol Content
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PBMC-MSC Co-cultures for Induction of Treg Generation
SM Sara M. Melief
CS C. L. M. Schrama
HR Helene Roelofs
Published: Vol 5, Iss 2, Jan 20, 2015
DOI: 10.21769/BioProtoc.1383 Views: 15248
Reviewed by: Hui ZhuNingfei An
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1,384 | https://bio-protocol.org/exchange/protocoldetail?id=1384&type=0 | # Bio-Protocol Content
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Monocyte-MSC Co-cultures
SM Sara M. Melief
CS C. L. M. Schrama
HR Helene Roelofs
Published: Vol 5, Iss 2, Jan 20, 2015
DOI: 10.21769/BioProtoc.1384 Views: 11590
Reviewed by: Hui ZhuNingfei An
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1,385 | https://bio-protocol.org/exchange/protocoldetail?id=1385&type=0 | # Bio-Protocol Content
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Isolation of CNS-infiltrating and Resident Microglial Cells
YJ Young-Hee Jin
BK Byung S. Kim
Published: Vol 5, Iss 2, Jan 20, 2015
DOI: 10.21769/BioProtoc.1385 Views: 15692
Reviewed by: Savita Nair
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... |
1,386 | https://bio-protocol.org/exchange/protocoldetail?id=1386&type=0 | # Bio-Protocol Content
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Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`-Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE)
MM Manuel Miras
RS Raquel N. Sempere
JK Jelena J. Kraft
WM W. Allen Miller
MA... |
1,387 | https://bio-protocol.org/exchange/protocoldetail?id=1387&type=0 | # Bio-Protocol Content
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A Conceptual Outline for Omics Experiments Using Bioinformatics Analogies
Prashanth Suravajhala
JB Jeffrey W. Bizzaro
Published: Vol 5, Iss 3, Feb 5, 2015
DOI: 10.21769/BioProtoc.1387 Views: 11456
Edited by: Arsalan Daudi
Rev... |
1,388 | https://bio-protocol.org/exchange/protocoldetail?id=1388&type=0 | # Bio-Protocol Content
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Water Deficit Treatment and Measurement in Apple Trees
CB Carole L. Bassett
TA Timothy S. Artlip
MW Michael E. Wisniewski
Published: Vol 5, Iss 3, Feb 5, 2015
DOI: 10.21769/BioProtoc.1388 Views: 8985
Edited by: Arsalan Daudi
... |
1,389 | https://bio-protocol.org/exchange/protocoldetail?id=1389&type=0 | # Bio-Protocol Content
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In vivo Heterotopic Bone Formation Assay Using Isolated Mouse and Human Mesenchymal Stem Cells
Li Chen
ND Nicholas Ditzel
Published: Vol 5, Iss 3, Feb 5, 2015
DOI: 10.21769/BioProtoc.1389 Views: 17239
Reviewed by: Salma Hasan... |
139 | https://bio-protocol.org/exchange/protocoldetail?id=139&type=1 | # Bio-Protocol Content
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Polyribosome Profiling
Wei Zheng
Published: Oct 5, 2011
DOI: 10.21769/BioProtoc.139 Views: 11612
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Acetone
Sucrose
Cyclohexamide
Bromophenol blue (BPB)
Urea
T... |
1,390 | https://bio-protocol.org/exchange/protocoldetail?id=1390&type=0 | # Bio-Protocol Content
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Detection and Quantification of Heme and Chlorophyll Precursors Using a High Performance Liquid Chromatography (HPLC) System Equipped with Two Fluorescence Detectors
JP Jan Pilný
JK Jana Kopečná
JN Judith Noda
RS Roman Sobotk... |
1,391 | https://bio-protocol.org/exchange/protocoldetail?id=1391&type=0 | # Bio-Protocol Content
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Application of Silicone Tubing for Robust, Simple, High-throughput, and Time-resolved Analysis of Plant Volatiles in Field Experiments
Mario Kallenbach
DV Daniel Veit
Elisabeth J. Eilers
Meredith C. Schuman
Published: Vol 5, ... |
1,392 | https://bio-protocol.org/exchange/protocoldetail?id=1392&type=0 | # Bio-Protocol Content
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Infection Experiments (Hepatitis C Virus)
Lidia Mingorance
Claudia Vasallo
Martina Friesland
Pablo Gastaminza
Published: Vol 5, Iss 3, Feb 5, 2015
DOI: 10.21769/BioProtoc.1392 Views: 9032
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1,393 | https://bio-protocol.org/exchange/protocoldetail?id=1393&type=0 | # Bio-Protocol Content
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Hanging Drop Aggregation Assay of Breast Cancer Cells
Yong Teng
Published: Vol 5, Iss 3, Feb 5, 2015
DOI: 10.21769/BioProtoc.1393 Views: 20084
Edited by: HongLok Lung
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1,394 | https://bio-protocol.org/exchange/protocoldetail?id=1394&type=0 | # Bio-Protocol Content
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A Filter Binding Assay to Quantify the Association of Cyclic di-GMP to Proteins
DS Disha Srivastava
CW Christopher M. Waters
Published: Vol 5, Iss 3, Feb 5, 2015
DOI: 10.21769/BioProtoc.1394 Views: 10649
Edited by: Fanglian H... |
1,395 | https://bio-protocol.org/exchange/protocoldetail?id=1395&type=0 | # Bio-Protocol Content
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Phosphatase Protection Assay: 14-3-3 Binding Protects the Phosphate group of RSG from λ Protein Phosphatase
Takeshi Ito
YT Yohsuke Takahashi
Published: Vol 5, Iss 3, Feb 5, 2015
DOI: 10.21769/BioProtoc.1395 Views: 9957
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1,396 | https://bio-protocol.org/exchange/protocoldetail?id=1396&type=0 | # Bio-Protocol Content
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Measuring Anxiety-like Behavior in Crayfish by Using a Sub Aquatic Dark-light Plus Maze
PF Pascal Fossat
JB Julien Bacqué-Cazenave
Jean-Paul Delbecque*
Daniel Cattaert*
*Contributed equally to this work
Published: Vol 5, Iss ... |
1,397 | https://bio-protocol.org/exchange/protocoldetail?id=1397&type=0 | # Bio-Protocol Content
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Protein Degradation Assays in Arabidopsis Protoplasts
Filip Mituła
Anna Kasprowicz-Maluśki
MM Michał Michalak
MM Małgorzata Marczak
KK Konrad Kuczyński
Agnieszka Ludwików
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/Bi... |
1,398 | https://bio-protocol.org/exchange/protocoldetail?id=1398&type=0 | # Bio-Protocol Content
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Preparation of Synaptosomes from the Motor Cortex of Motor Skill Trained Mice
Anand Suresh
Anna Dunaevsky
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1398 Views: 13864
Edited by: Soyun Kim
Reviewed by: Masah... |
1,399 | https://bio-protocol.org/exchange/protocoldetail?id=1399&type=0 | # Bio-Protocol Content
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Phenol-based Total Protein Extraction from Lily Plant Tissues
YC Yun-Chu Chen
YH Yu-Chun Hsiao
CW Co-Shine Wang
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1399 Views: 10982
Edited by: Arsalan Daudi
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14 | https://bio-protocol.org/exchange/protocoldetail?id=14&type=1 | # Bio-Protocol Content
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Protocol for Whole Cell Lysis of Yeast
Bio-protocol Editor
Published: Jan 5, 2011
DOI: 10.21769/BioProtoc.14 Views: 42777
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This protocol describes how to perfo... |
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Separation of Microspores from Anthers of Lilium longiflorum (Lily) and Subsequent RNA Extraction
ML Ming-Che Liu
YC Yun-Chu Chen
CW Co-Shine Wang
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1400 Views: 9742... |
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In vitro Chitin Binding Assay
Geneviève Ball
Frédéric Cadoret
Romé Voulhoux
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1401 Views: 12027
Original Research Article:
The authors used this protocol in Jul 2014... |
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Determination of Protein-DNA (ZMYND11-DNA) Interaction by a Label-Free Biolayer Interferometry Assay
YL Yuan-Yuan Li
HW Hong Wen
XS Xiao-Bing Shi
Hai-Tao Li
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1402 V... |
1,403 | https://bio-protocol.org/exchange/protocoldetail?id=1403&type=0 | # Bio-Protocol Content
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Isolation of Lung Infiltrating Cell in Mice
EA Eduardo P. Amaral
EL Elena B. Lassunskaia
MD Maria Regina D’Império-Lima
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1403 Views: 18953
Edited by: Fanglian He
Re... |
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Transmission Electron Microscopy for Tobacco Chloroplast Ultrastructure
Lina Yin
SW Shiwen Wang
NS Norihiro Shimomura
KT Kiyoshi Tanaka
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1404 Views: 10500
Edited by... |
1,405 | https://bio-protocol.org/exchange/protocoldetail?id=1405&type=0 | # Bio-Protocol Content
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Isolation of Chloroplast Inner and Outer Envelope Membranes
SW Shiwen Wang
Lina Yin
JM Jun’ichi Mano
KT Kiyoshi Tanaka
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1405 Views: 13743
Edited by: Tie Liu
Origina... |
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Macrophage Phagocytosis Assay of Staphylococcus aureus by Flow Cytometry
QY Qin Yan
SA Sun Hee Ahn
VJ Vance G. Fowler Jr
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1406 Views: 14842
Edited by: Fanglian He
R... |
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Whole Genome Bisulfite Sequencing and DNA Methylation Analysis from Plant Tissue
Daniela Pignatta
GB George W. Bell
MG Mary Gehring
Published: Vol 5, Iss 4, Feb 20, 2015
DOI: 10.21769/BioProtoc.1407 Views: 19662
Edited by: Ti... |
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Differentiation of Naturally Produced Extracellular Membrane Vesicles from Lipid Aggregation by Glucuronoxylomannan Immunogold Transmission Electron Microscopy in Bacillus subtilis
Lisa Brown
P Geoff Perumal
AC Arturo Casadev... |
1,409 | https://bio-protocol.org/exchange/protocoldetail?id=1409&type=0 | # Bio-Protocol Content
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Vesicle Isolation from Bacillus subtilis Biofilm
Lisa Brown
AK Anne Kessler
AC Arturo Casadevall
Published: Vol 5, Iss 5, Mar 5, 2015
DOI: 10.21769/BioProtoc.1409 Views: 12535
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141 | https://bio-protocol.org/exchange/protocoldetail?id=141&type=1 | # Bio-Protocol Content
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GST-tagged Yeast Protein Purification
HI Hogune Im
Published: Oct 5, 2011
DOI: 10.21769/BioProtoc.141 Views: 16883
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Glutation S-transferase (GST) tagging is the most commo... |
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