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cc-by | In this work, we compare vertical column density of water vapour measured at Observatoire de Haute-Provence, Southern France ( 5 • 42 E, +43 • 55 N). Data were obtained by three satellite sensors, GOME, GOME 2 and SCIAMACHY, and by two groundbased spectrometers, Elodie and SAOZ. These five instruments are able to meas... | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Promising infrared techniques (e. g. MERIS, IASI) could be used in the future, however, the differences about techniques, characteristic, and algorithms will be a next step used beyond the scope of this study. This article is structured as follows: Sect. 2 presents a description of these instruments; Sect. 3 describes ... | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Line-ofsight values of individual observations are divided by the air-mass factor of the observation, the coefficient needed to obtain vertical amounts. This technique is the same as the one used to measure stratospheric constituents like ozone and NO 2 but extended for tropospheric constituents like water vapour. Note... | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | One of the objectives of this publication is therefore to bring quality feedback from the community about this data set and therefore to change its status and fill up the corresponding SAOZ database. | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Results and discussion For this study we analysed and compared two VCD data sets of H 2 O above OHP. The satellites data sets are issued from SCIAMACHY, GOME and GOME2. The groundbased instruments are Elodie and SAOZ. Table 2 presents the temporal coverage for these data sets for each instrument. We must indicate tha... | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Inter-comparison of ground-based measurements Monthly averages of the total column water vapour measured by SAOZ and Elodie, from 1995 to 2004 above OHP, both exhibit a clear annual variation (Fig. 2 ). SAOZ can measure H 2 O even in the presence of clouds, in contrast to Elodie which measures only by clear skies. SAOZ... | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | 2 ), however, with small differences which appear sometimes during maximum or minimum values. The correlation coefficient is R = 0. 8, and indicates a high correlation between the two types of measurements. We can see also note that for the year 2003 in the SAOZ dataset, the maximum of the seasonal cycle of this year i... | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Comparison between ground-based and satellite measurements at OHP In order to examine the representation of the satellite estimates of H 2 O above OHP compared to the ground-based measurements, we compared the two types of data sets. As we mentioned above, the three satellites of this study utilise the AMC-DOAS algorit... | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Period of measurements Table 4 presents the time of measurements for each instrument in summer and in winter. According to this table we can see that there is a significant discrepancy between the time of measurements of ground-based instruments and the overpass times of the satellites above OHP, but also between the... | 10.5194/amtd-6-4249-2013 | preprint | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Process of bioinformatics study Our study was performed in two steps: (i) the nucleotide sequences of the autophagy genes-Atg5, Becn1, Atg7, Atg10, Atg12, Atg16l1, Gabarap, Gabarapl2, Map1lc3b, Nbr1, p62 (Sqstm1)-were imported from various mammalian species (EnsEMBL database, http://www. ensembl. org/) using the Ensemb... | 10.1093/nar/gkt563 | article | en | 2,013 | true | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Scanning of the promoter sequences with IUPAC patterns (Genomatix Software GmbH, Munich, Germany) revealed that the 5 0 flanking region of these genes contains a putative AARE sequence (5 0 -TGATG MMAH-3 0 ) (Supplementary Figure S3 ). ChiP assays performed with primer sets covering the AARE, anti-ATF4 and anti-CHOP a... | 10.1093/nar/gkt563 | article | en | 2,013 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | In CHOP-ATF4 double-ChIP assays, DNA fragments were first immunoprecipitated with CHOP antibody, eluted, and then subjected to the second immunoprecipitation with ATF4 antibody or normal rabbit IgG antibody. The enrichment of CHOP/ATF4 proteins was analyzed by qPCR using primer sets specific for Nbr1 and Atg7 promoters... | 10.1093/nar/gkt563 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Data were plotted as the percentage of antibody binding versus the amount of PCR product obtained using a standardized aliquot of input chromatin (% of Input). In CHOP-ATF4 double-ChIP assays, DNA fragments were first immunoprecipitated with CHOP antibody, eluted, and then subjected to the second immunoprecipitation wi... | 10.1093/nar/gkt563 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | ACKNOWLEDGEMENTS We are grateful to Drs D. Ron and H. Harding ( Institute of Metabolic Science, Cambridge, UK ) for the gift of ATF4-and CHOP-deficient MEFs and CHOP expression plasmids. The authors thank Drs D. Be´chet , A. Darfeuille-Michaud and J. C. Pointud for helpful discussions. FUNDING Institut Nati... | 10.1093/nar/gkt563 | article | en | 2,013 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Data were plotted as the percentage of antibody binding versus the amount of PCR product obtained using a standardized aliquot of input chromatin (% of Input). In CHOP-ATF4 double-ChIP assays, DNA fragments were first immunoprecipitated with CHOP antibody, eluted, and then subjected to the second immunoprecipitation wi... | 10.1093/nar/gkt563 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | PDB codes 1o8a (ligand-free) and 2xy9 (FII-bound) used for C-ACE; 2c6f (ligand-free) and 2xyd (FII-bound) for N-ACE; 2x8y for ligand-free AnCE. Rmsd of all atoms for main chain/side chain ( A) share similar K i for C-ACE and thus the presence of FII-B at the allosteric site does not seem to influence the inhibition pot... | 10.1111/febs.12660 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Data reduction was carried out by using the CCP4 program SCALA [54]. Initial phases for structure solution were obtained using the molecular replacement routines of the PHASER program [55]. The atomic coordinates of C-ACE (PDB code 1O8A [26] ), N-ACE (PDB code 2XYD [43] ) and AnCE (PDB code 2X8Y, [42]) were used as... | 10.1111/febs.12660 | article | en | 2,013 | true | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Validation was conducted with MOLPROBITY [59]. Figures were drawn with PYMOL (DeLano Scientific, San Carlos, CA, USA). Hydrogen bonds were verified with the programs HBPLUS [60] and LIGPLOT [61]. The detailed refinement statistics for the complex structure are given in Table 1. The atomic coordinates and the struc... | 10.1111/febs.12660 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgements This work was supported by the Medical Research Council (UK) through a project grant (number 81272 ) and the Wellcome Trust (UK) equipment grant (number 088464 ) to K. R. A. We thank the scientists at stations I02, I03, I04, I04-1 of the Diamond Light Source, Didcot, Oxon (UK) , for their suppor... | 10.1111/febs.12660 | article | en | 2,013 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | aegypti genome assembly (AaegL 1. 1 annotation) using TAGMATCHER, a software program based on the short sequence mapping algorithm 'agrep' (Wu and Manber 1992). TAGMATCHER makes it possible to match tags to a reference genome with errors and multiple matching loci (available on request from eric. coissac@inrialpes. fr... | 10.1111/eva.12082 | article | en | 2,013 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Genotyping was performed at the Genotyping Center GENTYANE in Clermont-Ferrand, France http://www. ibisa. net/plateformes/GENTYANE,101. html) according to the standard protocol for the DNA Illumina GoldenGate â array (Fan et al. 2006) , and data were analysed using the software program Genome Studio â v. 1. 5. 10 (Ill... | 10.1111/eva.12082 | article | en | 2,013 | true | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Candidate gene identification, sequencing and neutrality tests Candidate genes for Bti resistance were identified using a keyword search in the VectorBase database http://aaegypti. vectorbase. org/index. php. A total of 162 genes belonging to families of toxin receptors (14 alkaline phosphatases, 23 aminopeptidases, 17... | 10.1111/eva.12082 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | We also sequenced as a negative control three candidate genes described in literature as binding Cry toxins (the aminopeptidase AAEL012778, the cadherin AAEL007488 and the alkaline phosphatase AAEL009077; Chen et al. 2009; Likitvivatanavong et al. 2011b ) but located in genomic regions with low F st between the suscep... | 10.1111/eva.12082 | article | en | 2,013 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | 2 lM of each primer, 5 lg BSA, 0. 6 U AmpliTaq Gold DNA polymerase (Applied Biosystems, Foster City, CA, USA) and 50 ng DNA. The PCR program was initial 10 min denaturation step at 95°C; 40 cycles of denaturation at 95°C for 45 s, annealing at 58°C for 45 s and elongation at 72°C for 60 s; and a final extension step at... | 10.1111/eva.12082 | article | en | 2,013 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgements This study was supported by grants from the R egion Rhône-Alpes (grant # 0501545401 ), the French National Research Agency (grant ANR-08-CES-006-01 DIBBECO ), the French Agency for Environmental Health and Safety ( 2007-CRD-55 ) and the Institut de Recherche pour le Developpement. We thank the ... | 10.1111/eva.12082 | article | en | 2,013 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Data archiving statement Next-generation sequencing data and cDNA library information associated with the present study have been deposited at the EMBL-EBI European Read Archive (ERA) under accession number ERA000115 and are freely accessible at http://ftp. era-xml. ebi. ac. uk/ERA000. Sequence data for this. | 10.1111/eva.12082 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | As shown in Figure 4 , gene expression was strongly inhibited by MuV-V, whereas TioV-V, TioV-P or TioV-W had no effect. Altogether, we demonstrated that in contrast to MuV-V, TioV-V or known products of the P gene like TioV-P and TioV-W are unable to block IFN-a/b signaling in human cells. This suggested that TioV-V i... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Using this protocol, we identified MDA5, LGP2 and STAT3 as direct interactors of TioV-V. The interactions with MDA5 and LGP2 were previously (B) Luciferase expression was determined at 24 h and 48 h post-infection. (C) Culture supernatants from (B) were collected at 48 h post-infection, clarified by centrifugation, UV-... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | All experiments were performed in triplicates, and data represent means 6 SD. *indicates that differences observed relative to controls (none) with IFN-b, IL-6, MDA5 or IFN-l were statistically significant (p-value,0. 01). doi:10. 1371/journal. pone. 0053881. g003 DDB1. After 48 h, viral proteins were purified with glu... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | We then investigated the induction of STAT1 degradation and the binding of TioV-V or MuV-V to endogenous STAT2. HEK-293T cells were transfected with expression vectors encoding for 3xFLAG-tagged TioV-V, TioV-P, TioV-W or MuV-V, and. Expression of IFN-inducible genes is impaired by MuV-V but not TioV-V expression. HEK-2... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Experiment was performed twice and data represent means 6 SD. *indicates that differences observed with MuV-V relative to controls cells transfected with 3xFLAG alone and stimulated with IFN-b were statistically significant (p-value,0. 05). doi:10. 1371/journal. pone. 0053881. g004 endogenous STAT1 expression was deter... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | From total RNA extracts, we were able to amplify, clone and establish the sequence of STAT2 from P. rodricensis ( Pr STAT2). A draft of Pteropus vampyrus genome has also been established by the Human Genome Sequencing Center, the Baylor College of Medicine and the Broad Institute, and is available on Ensembl database. ... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Plasmid DNA Constructs TioV-V (NP_665866), TioV-P (NP_665865), TioV-W (NP_665867), TioV-VCT (AA 150-228 of TioV-V), MuV-V (ABG48763, Mumps virus isolate ''Sophie'', GenBank accession number: DQ660370) and NiV-V (NP_112023. 1) coding sequences were amplified by RT-PCR (Titan One tube; Roche) from RNA samples kindly prov... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Yves Jacob). DNA fragment encoding for Pr STAT2 was generated from total RNA purified from Pteropus rodricensis blood. Samples were obtained as part of a routine medical check up performed on animals of a bat colony maintained in La Palmyre zoo. Blood samples were collected by the veterinarian in charge following appro... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | After 48 h, relative luciferase activity was determined. (D) Experiment was performed as in (A), but cells were co-transfected with expression vectors encoding 3xFLAG-tagged TioV-P or TioV-W. (E) Experiment was performed as in (A), but cells were co-transfected with pCI-neo expression vector, either empty or encoding u... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments We would like to thank Prof. S. K. Lam for providing TioV, and Dr. TF. Wild for RNA samples from TioV and MuV infected cells. We thank all members of PF1-Pasteur Genopole sequencing core facility. We thank members of the Infection- MAPping project I-MAP for fruitful discussions. | 10.1371/journal.pone.0053881 | article | en | 2,013 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | After 48 h, relative luciferase activity was determined. (D) Experiment was performed as in (A), but cells were co-transfected with expression vectors encoding 3xFLAG-tagged TioV-P or TioV-W. (E) Experiment was performed as in (A), but cells were co-transfected with pCI-neo expression vector, either empty or encoding u... | 10.1371/journal.pone.0053881 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Update on Clinical Trials When our latest Trial Watch dealing with DC-based anticancer interventions was submitted for publication to OncoImmunology (July, 2012), official sources listed 114 recent (started after 2008, January 1st) clinical trials (all statuses included) that would assess the safety and efficacy of thi... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | clinicaltrials. gov). Preliminary results from NCT01373515, a Phase I/IIa clinical trial investigating the safety and therapeutic potential of DCP-001, a preparation of mDCs obtained from an acute myeloid leukemia (AML)-derived cell line that expresses multiple TAAs (so-called "DCOne" cells), have been disclosed at the... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | At present (July 2013), official sources list 29 clinical trials launched after 2012, July 1st that would investigate the safety and therapeutic profile of DC-based anticancer interventions (source www. clinicaltrials. gov). The most common approach in this sense is represented by the administration of autologous DCs e... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Along similar lines, the safety and clinical profile of autologous DCs pulsed ex vivo with not better specified TAAs or TAAderived peptides, administered in combination with the hitherto experimental TLR3 agonist Hiltonol ® 219,343 or with hematopoietic stem cells plus cytotoxic T lymphocytes, are being assessed in a... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Five studies have recently been launched to investigate the clinical profile of autologous DCs pulsed ex vivo with tumor lysates. This approach, is being evaluated either as a standalone therapeutic intervention or combined with gemcitabine (an immunostimulatory nucleoside analog) 65, 66, 355, 356 and/or the FDA-appr... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Four of these studies involve the reinfusion of autologous DCs expanded ex vivo (in the presence of appropriate maturation signals, but not genetically modified), either as a standalone therapeutic interventions or in combination with Hiltonol ® , to individuals affected by Stage III melanoma (NCT01875653), unresectabl... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Finally, the efficacy of DCs transfected ex vivo with tumor-derived or TAAcoding RNAs, invariably as standalone therapeutic interventions, is being investigated in 3 clinical trials. In particular, DCs transfected with mRNAs coding for Wilms' tumor 1 (WT1) or several other leukemia-associated antigens are being explore... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | 7] [108] Nowadays, several of these DC-based anticancer interventions have entered the clinical phase of development. Official sources list indeed more than 120 clinical trials recently (after 2008, January 1st) initiated to test the safety and efficacy of DC-based anticancer immunotherapy (source www. clinicaltrials.... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Acknowledgments Jitka Fucíková works for Sotio a. s. ( Prague, Czech Republic ), which is actively investigating DC-based vaccines in both preclinical and clinical settings. Authors are supported by the European Commission (ArtForce) ; European Research Council (ERC) ; Agence National de la Recherche (ANR) ; Ligue ... | 10.4161/onci.25771 | review | en | 2,013 | false | false | true | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | a. As single agent NCT01753089 Sarcoma I I/II recruiting recruiting n. a. n. a. Combined with imiquimod ± gemcitabine NCT01803152 As single agent NCT01883518 Breast carcinoma I II/IIII recruiting Active not recruiting erBB2 n. s. As single agent Combined with CTLs and HSCT NCT01730118 NCT01782274 CrC I/II recruiting Ce... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | s. As single agent NCT01676779 I recruiting PAP Combined with ipilimumab NCT01832870 II Not yet recruiting PAP As single agent or in combination with eBrT NCT01807065 Sipuleucel-T Prostate cancer II recruiting PAP Combined with a DNA-based vaccine and GM-CSF NCT01706458 II Active not recruiting PAP Combined with ipilim... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | a. , not applicable/not available; n. s. , not specified; PAP, prostatic acid phosphatase; PBL, peripheral blood lymphocyte; rCC, renal cell carcinoma; rh, recombinant human; SABr, stereotactic ablative radiation therapy; TAA, tumor-associated antigen; TBvA, tumor blood vessel antigen; TCr, T-cell receptor; wT1, wilms'... | 10.4161/onci.25771 | review | en | 2,013 | false | false | false | true | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Statistical analysis A database was built using Excel 7. 0 (Microsoft) software. MRI findings and CSF/serum HP10 levels were recorded. Statistical analysis was made using SPSS 10. 0 (Microsoft), Epidat 3. 0 and SAS 9. 0 softwares. Parametric statistics (mean and SD) were calculated. Kappa coefficient (k) and 95% confid... | 10.1371/journal.pntd.0002096 | article | en | 2,013 | true | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Over the past ten years technologies for generating, maintaining and using 3D geo-information have matured. For national mapping agencies one of the challenges is how to best extend 2D data into 3D data, making best use of research results and available technologies. Some mapping organisations are making serious progre... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | INTRODUCTION Over the past ten years technologies for generating, maintaining and using 3D geo-information have matured. Many local governments have 3D models of their area, a large number of companies are providing services for creating or disseminating 3D models, and universities and research organisations are invest... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | 0) has been expanded and now supports a variety of objects in landscape and city models. Many projects use CityGML for 3D modelling. For example North-Rhine Westphalia in Germany has been the first state that provides a statewide 3D model consisting of 3D roads, railways, DTM, and 3D buildings employed for noise disper... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Some examples are noise (as used in the German state North-Rhine Westphalia and as documented within the CityGML specification), utility networks (Becker et al, 2010; 2013) , real estate management, robotics, building information models (BIM), and hydrography (see CityGML, 2013). It is clear that 3D applications are ... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | The objectives of the workshop were to discuss the benefits and barriers to the provision of 3D geospatial data and related services by NMAs, explore the relationship between 2D and 3D data, identify the potentials of the CityGML standard and define requirements for future developments of the standard. The workshop mat... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | 3D MAPPING AT ICC: 3D DATA FOR TOPOGRAPHIC PRODUCTS The Institut Cartogràfic de Catalunya (ICC) is the regional mapping agency of the Government of Catalonia. From its creation in late 1982, the ICC has focused improving the cartographic production workflows. Topographic data, ranging from scales 1:1000 to 1:25. 000, a... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | 3D Topographic Data in urban areas The topographic data set at 1:1000 scale (CT-1M) is the most detailed data covering the urban areas and the areas planned for urbanization, a total surface of 330. 000 hectares. The accuracy of this product is 20 centimeters for X and Y and 25 centimeters for height. The data is compi... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | 5D data model and stored in an ORACLE database. The accuracy is 1 meter for X and Y and 1. 5 meters for the height. During the stereoplotting process all the features required to generate a digital terrain model (DTM) and a digital surface model (DSM) are compiled together with the topographic objects (Figure 3 ). The... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | The Topographic Database at 1:25. 000 scale Although from the beginning of 2000's a complete coverage of BT-5M was available, the customers were asking for a database at smaller scale for new GIS applications and mapping. This database should be manageable, like the 1:50. 000 database, but with the topographic informat... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Comparing with previous ICC generalization experiences, the workflow entailed two challenges: to obtain a topographic database, not only a map, and to generalize 2. 5D data instead of 2D data (Figure 4 ). The complete coverage was finished in 2011 and, nowadays, the database is being updated independently of the origi... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Work in progress The first line of the 3D work in progress is focused in the enrichment of the CT-1M data, adding the inclined roofs for obtaining a LOD2 city models. After some tests on commercial software for obtaining inclined roofs automatically, based on image correlation and building boundaries, the results are p... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | The third line of work is related to maintenance and updating of the information, crucial for optimizing the production costs. The related tasks in development are the coordination with municipalities and other government organizations to share information to speed the change detection. Other line of work is the analys... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | 3D MAPPINT AT ORDNANCE SURVEY: FROM 2D TO 3D -EXPERIENCES AND CHALLENGES As the National Mapping Agency for Great Britain, Ordnance Survey's products include address data, digital terrain models (DTMs), road networks, imagery and topographic mapping (vector and raster), including the flagship topographic vector mapping... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | A 3D future for Ordnance Survey? The long-term plan is to '3D-enable' the core databases, products and services. This will involve three broad types of change (see Figure 5 ): • Creating a new generation of elevation models (completed) • Adding ground height values to 2D vertices and features in the existing 2D datase... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Datasets New elevation models DTMs (complete) DSMs Ground heighting of existing 2D datasets. | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Principle Narrative Survey standards Data will be captured to defined standards of geometric and attribute accuracy Maintained Features will be revised and updated throughout their lifecycle through a combination of continuous and cyclic processes Nationally consistent Equivalent geographies will (over time) be publish... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Technical barriers Firstly, automated data capture techniques are preferred for minimising time and cost. This is especially relevant when scaling up to a city or national level. 3D data must also be captured during the same revision cycles as the existing 2D datasets in order to ensure synchronicity and interoperabili... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Organisational barriers Ordnance Survey has for over 200 years produced 2D data. This has resulted in a certain level of learned behaviour and in a legacy of products defined in terms of 2D data specifications and representations. Consequently a process of review is necessary internally in order to gain a better unders... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | CityGML for Ordnance Survey Feedback has shown that customers see Ordnance Survey as a prime candidate for promoting the use of 3D data through internationally recognised standards. CityGML fits this bill very neatly. The OGC standard CityGML is one of the few (if not only) internationally recognised standards for 3D g... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | g. from data capture to database environment. • As a transfer format for data supply to our customers While there are many advantages to using CityGML, OSGB also perceive some challenges, in particular: • The need to define an accepted national profile for CityGML that is • Fully supported by software vendors in order ... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | In addition, TOP10NL is less detailed than IMGeo and therefore better suitable for fully automatic 3D object reconstruction since fewer details are present (therefore less special cases). We assume that the resulting dataset is sufficient for a nationwide dataset (i. e. acceptable performance for nationwide application... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | 3D MAPPING IN SWITZERLAND The Swiss Federal Office of Topography (swisstopo) offers a wide range of products ranging from traditional paper maps to digital multimedia products. Since 2008 swisstopo has been developing and updating the Topographic Landscape Model (TLM). TLM is the basis landscape model for Switzerland. ... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Geometries and a wide range of attributes are provided for all landscape features. All objects have x, y and z coordinates which also means that objects in TLM maintain consistency with DTM-TLM, for example 3D roads should lie on the DTM. TLM is not a product, but rather will be the basis for a wide variety of products... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Production and updating The ever-increasing demand for better, more up-to-date diverse digital data has meant that the rapid updating of swisstopo products has taken on a high importance. With the new established production process for TLM an updating infrastructure for a central data set from which all products could ... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Natural and artificial landscape features Compared with the formerly existing geodata (VECTOR25, SwissNames, etc. ) the TLM offers higher geometric accuracy, but is also more extensive and up-to-date. The existing data was migrated into the TLM as a basis, mainly due to the rich information in attributes and topology m... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | 3D Products Various 3D data sets extracted from the TLM are available since autumn 2010. They comprise the entire territory in reduced form -measured according to the final requirements of the TLM. To be more precise, the number of landscape features on offer is reduced compared with the TLM landscape feature catalogue... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Pending issues as for true 3D data models As mentioned above, automated processes are good at creating the bulk of a city model, but failures are costly to identify, and corrective editing can be painstakingly long. Further work on automated identification of failures, and on assisted editing tools are therefore underw... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | CityGML and IGN-F 3D data products Insofar CityGML has proved to be up to the task, robust enough to provide a good basis for 3D data exchanges. Only ongoing research suggests that further developments are needed. This being said, many users still require data to be provided in a different data model, because of limite... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Conclusions From the approaches described in this paper, several conclusions can be drawn. Firstly, 3D data is more and more available but still suffers from a low level of usage (mainly visualisation). In addition standards and formats based on CityGML have been stabilised although software support is still in the ear... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | For the digitising of maps resulting in 2D databases, the benefits were clear: initially production lines were improved and later the digitised point, lines and areas could be upgraded to 2D information needed in spatial analyses. In contrast, 3D does not contribute to the improvement of an existing process. Instead 3D... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | The 3D profile of the INSPIRE Data Specifications for Buildings, which is based on CityGML, is a good example. We need more of such implementation profiles to offer a solid investment foundation for both organisations and the market. | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Further work Now 3D techniques have matured and OGC has established a standard for 3D data which is well stabilised, in many countries it is no longer a question if countrywide 3D mapping should be realised, but "when" and "how". From the findings in this paper several issues can be identified that require further (res... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | Firstly, CityGML needs further support in software systems. This includes implementation of standard products and development of open source tools to support operations. In addition, as a generic standard CityGML needs further agreements defined in profiles. Therefore European CityGML profiles (as the INSPIRE Building ... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | • More insights in real customer needs of 3D data sets are required (i. e. what topics are relevant and to what extent). These needs should be formulated in terms of what problems 3D can solve. This will stimulate other use of 3D data than visualisation. • More research is needed on how to fuse data, combining existing... | 10.5194/isprsannals-ii-2-w1-269-2013 | article | en | 2,013 | true | false | false | false | Engineering | https://openalex.org/fields/22 |
cc-by | miRNAs regulate gene expression at the posttranscriptional level (1, 2). To date, $1500 human miRNAs have been documented in the database of miRNAs, miRbase (23). miRNAs are believed to regulate the expression of upwards of 30% of human genes by enabling RISC to recognize complementary sequences in target messenger R... | 10.1093/nar/gkt086 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Sedimentation velocity analytical ultracentrifugation Sedimentation velocity experiments were performed with a Beckmann Coulter XLI analytical ultracentrifuge at 50 000 rpm (185 000g), 25 C with an An-50Ti rotor. Samples or reference (NMR buffer) were loaded into sapphire-windowed cells with 12-mm optical paths, which ... | 10.1093/nar/gkt086 | article | en | 2,013 | true | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | 52; (42)] using the c(s) analysis and non-interacting species models. The latter was used to estimate buoyant molar masses, M b , using the equation, M b = M  [1 À (r  " v)], where M is the molar mass and r the solvent density. Hybrid models and multiwavelength analysis were performed in the program SEDPHAT (43). Re... | 10.1093/nar/gkt086 | article | en | 2,013 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Isothermal titration calorimetry All isothermal titration calorimetry (ITC) experiments were conducted using a MicroCal ITC-200 at 25 C. ITC experiments were performed in two ways: either with the 'ligand', in this case the protein, in the cell, or with 'ligand' in the syringe. Protein concentrations varied between 30 ... | 10.1093/nar/gkt086 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | The resulting binding isotherms were fitted to maximize the likelihood of the data assuming a Gaussian noise distribution. The titration data were globally fitted for each pair of experiments using previously described protocols (44). The standard deviation of the estimation of each binding parameter was determined by... | 10.1093/nar/gkt086 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Taken together, these data suggest that the RNA-binding region of TRBP is composed of two independent dsRBDs connected by a flexible linker and, therefore, that it is similar to the tandem dsRBDs that form the dsRNA-interacting region of PKR (45). Both dsRBDs of the RNA-binding region of TRBP interact with pre-miR-155... | 10.1093/nar/gkt086 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Furthermore, the macroscopic binding enthalpy obtained for TRBP-D12 is approximately equal to the sum of that of TRBP-D1 and TRBP-D2, which indicates that the polar interactions formed by each single domain and pre-miR-155 are unaffected in the context of the full-length protein. Thus, these ITC data support the conclu... | 10.1093/nar/gkt086 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | 35. Non-interacting species analysis gives a molecular mass of 18. 5 kDa, which is again close to the theoretical value of 19. 4 kDa. An additional minor contribution at 7. 2 S (6% of the main peak) was also detected. This peak is likely to result from a small fraction of dimerized pre-miR-155. This species was also ob... | 10.1093/nar/gkt086 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | ACKNOWLEDGEMENTS The authors thank Drs. A. Favier and E. Condamine for help with the acquisition of NMR data; Drs. R. Rasia , B. Bersch , C. Petosa and A. Leech for critical reading of the manuscript; and the Grenoble Partnership for Structural Biology (PSB) for access to integrated structural biology platf... | 10.1093/nar/gkt086 | article | en | 2,013 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgements. This work was undertaken under the MI-AVITA project, financed by the European Commission under the 7th Framework Programme for Research and Technological Development, Area "Environment", Activity 6. 1 " Climate Change, Pollution and Risks ". The authors would like to thank A. Vagner , J. Dougl... | 10.5194/nhess-13-1929-2013 | article | en | 2,013 | false | false | true | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Another study identified a universal set of residues, I,V,Y,W,R,E,L (IVYWREL measure), enriched in thermophiles [19]. More complex discrimination functions have also been proposed, such as the function employed by THERMORANK [20]. This method uses a linear combination of 10 sequence-based features to rank a set of in... | 10.1186/1745-6150-8-2 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | This bias was interpreted as a sign of protein thermostability [5]. Another EST resource, generated by the Joint Genome Institute (JGI, http:// www. jgi. doe. gov/), is also publicly available. These A. pompejana EST resources could form the basis for reconstituting and determining structures of metazoan proteins and ... | 10.1186/1745-6150-8-2 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Deep sequencing of the A. pompejana transcriptome To gain insights into the mechanisms of thermoadaptation of A. pompejana, we first performed deep sequencing of its transcriptome. We used a combination of the Sanger, Roche/454 and Illumina technologies. With the 454 technology we obtained 2,717,445 reads of an average... | 10.1186/1745-6150-8-2 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | To estimate the number of novel sequences in our resource (MPI dataset, Additional file 1) we compared it with the already available ESTs (JGI + Genoscope dataset, Additional file 2), as well as the combined dataset (MPI + JGI + Genoscope dataset, Additional file 3). First, we compared the length distribution of contig... | 10.1186/1745-6150-8-2 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | We then compared the A. pompejana predicted proteins in the MPI + JGI + Genoscope dataset to those already available in the JGI + Genoscope dataset. We found 13,301 sequences (>100 aa) in the MPI + JGI + Genoscope dataset with no identical BLAST hit (fraction identical <90%) to the JGI + Genoscope dataset. We then perf... | 10.1186/1745-6150-8-2 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | In a BLASTP comparison of the predicted protein datasets, 2,776 query sequences from the MPI + JGI + Genoscope dataset were at least 40 amino acids longer than their corresponding fragments in the JGI + Genoscope dataset. Next, we annotated the combined predicted A. pompejana proteome using BLASTP (Additional file 8). ... | 10.1186/1745-6150-8-2 | article | en | 2,013 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
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