license stringclasses 1
value | text stringlengths 66 12.2k | doi stringlengths 10 59 | type stringclasses 14
values | detected_lang stringclasses 6
values | publication_year float64 2.01k 2.02k | is_dataset bool 2
classes | is_software bool 2
classes | is_acknowledgement bool 2
classes | is_clinicaltrial bool 2
classes | field_name stringclasses 26
values | field_id stringclasses 26
values |
|---|---|---|---|---|---|---|---|---|---|---|---|
cc-by | 2. 3). We compute the model monthly mean by sampling only those days when AERONET data are available. We use available stations with measurements for the year 2010. Stations above 1000 m a. s. l. are excluded since we do not correct the model AOD for the station altitude. For the analysis with respect to the MODIS data... | 10.5194/acp-13-6555-2013 | article | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Acknowledgements. This study was co-funded by the European Commission under the EU Seventh Research Framework Programme (grant agreement No. 283576 , MACC II) and the French Ministère de l'Ecologie, du Développement Durable, des Transports et du Logement (MEDDE) under the GMES-MDD programme. The authors are gr... | 10.5194/acp-13-6555-2013 | article | en | 2,013 | false | false | true | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Flow cytometry Antibodies for fluorescein isothiocyanate (FITC)-conjugated anti-CD123 or BDCA-2 (Miltenyi Biotec, Auburn, CA), Phycoerythrin (PE)-conjugated CD11c (IOTest/Beckman Coulter, Marseille, FR), Allophycocyanin (APC)-conjugated anti-BDCA-4 (Miltenyi Biotec) and Allophycocyanin-Cy7 (APC-Cy7)-conjugated anti-CD1... | 10.1371/journal.pntd.0002257 | article | en | 2,013 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | CD3 2 CD4 + CD14 2 CD123 + /BDCA-2 + BDCA-4 + gated cells were then tested for the expression of surface markers using PE-labeled anti-TRAIL (BD Biosciences). Mosquito C6/36 cell line monolayers were washed with PBS-1% bovine serum albumin (Sigma) and incubated for 60 min at 4uC with purified anti-DENV-complex (Millipo... | 10.1371/journal.pntd.0002257 | article | en | 2,013 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Statistical analysis Experiments were repeated at least four times. P values (P) were determined using a two-tailed Student's t test for in vitro data and nonparametric Mann-Whitney test for patient data. P,0. 05 was considered statistically significant. Univariate distributions of flow cytometric data were performed b... | 10.1371/journal.pntd.0002257 | article | en | 2,013 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Dengue patients differentially exhibit TRAIL + pDCs, soluble IFN-a and TRAIL levels We studied a cohort of DENV infected patients and classified them regarding the severity of the disease. Detailed demographic, clinical, and laboratorial data from dengue patients are summarized in Table 1. From 43 patients enrolled, 1... | 10.1371/journal.pntd.0002257 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | DENV-2 and TRAIL location within pDCs by 3-dimension microscopy To better characterize DENV-2-activated pDCs, we analyzed them by 3-dimension (3D) microscopy. Focal plane analysis revealed the presence of intracellular TRAIL expression in unstimulated pDC (figure 3A , upper panels), confirming our cytometry data and o... | 10.1371/journal.pntd.0002257 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | The blocking of endosomal acidification by chloroquine use revealed the same profile as mock-stimulated pDCs. Thus, to better characterize TRAIL localization in pDCs, 3D reconstruction (focal plan, XZ and YZ-stacks) analysis was performed (figure 3B-D ). 3D interactive surface plot plugin of ImageJ software combined w... | 10.1371/journal.pntd.0002257 | article | en | 2,013 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments We thank Amanda Torrentes de Carvalho , Cintia Marinho , Debora Batista de Oliveira , Ana Rita Motta Castro for their assistance with patient recruitment and sample collection. We also thank Iris Peixoto Alvim and Geraldo Pereira for the help in the FACS Aria Facility, FIOCRUZ. We acknowledge ... | 10.1371/journal.pntd.0002257 | article | en | 2,013 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | The photodetector is scanned along the bearing surface of the girder and along the module at the radius at which the inner radius shims are glued. The data were recorded and used to determine the shim thicknesses to be used for the interface between each module pair. This is the nominal shim thickness. It was used for ... | 10.1088/1748-0221/8/11/t11001 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | More detail can be found in [23]. Due to the self-supporting feature of the design of the Tile Calorimeter, and the size of the structure, the assembly of the calorimeters began with a full surface assembly to establish the validity of the planned assembly approach prior to installation in the cavern [24]. Geometrica... | 10.1088/1748-0221/8/11/t11001 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Several measurement techniques were used to monitor the geometry of the calorimeter during pre-assembly, all of which used the fiducial marks described above as the reference points. The techniques comprised: a custom precision ruler used to measure the chord length between opposite modules; optical survey of the posit... | 10.1088/1748-0221/8/11/t11001 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Transverse to the beam line the calorimeters are on axis within the precision of 1 mm provided by the calorimeter guide brackets. A full compilation of these results is available in the EDMS database [27]. In conclusion, following an odyssey of over 12 years the ATLAS Tile Calorimeter was designed, constructed and ins... | 10.1088/1748-0221/8/11/t11001 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Acknowledgments This report documents the construction of the ATLAS Tile Calorimeter from design to installation in the ATLAS cavern. This work brought together scientists engineers and technicians from 3 continents, who had the privilege and pleasure to be part of this effort; a special thanks to them, including the C... | 10.1088/1748-0221/8/11/t11001 | article | en | 2,013 | false | false | true | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | HFRS and climatic data Records of HFRS cases from 1991 to 2010 were obtained from the the Hunan Notifiable Disease Surveillance System (HNDSS). The data were from a passive surveillance system and all HFRS cases were first diagnosed by clinical symptoms, as defined by a national standard [7]. Clinical diagnosis criter... | 10.1371/journal.pntd.0002260 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | The results most likely indicate that moisture not only influences growth of food sources that determine rodent population size, thereby affecting the HFRS transmission, but also directly influences rodent activity and hantavirus infectivity. These findings offer insights in understanding possible causes of HFRS transm... | 10.1371/journal.pntd.0002260 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | " Discussions of logistics and routines for sequencing and assembling various arthropod genomes were extensive and it was evident that many researchers were moving ahead with generating sequences for their favored species. Standardized protocols and data pipelines were identified as pressing needs. These pipelines woul... | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | org/wiki/i5K_working_groups). As the workshop ended, i5K leader Dr. G. Robinson provided a bridge by delivering the Plenary introduction to the 6th Arthropod Genomics Symposium, a venue for discussing progress and tools in arthropod research. Since the workshop, Dr. K. Hackett of the USDA's Agricultural Research Servic... | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | g. , iPlant consortium; http://www. iplantcollaborative. org) to discuss joint efforts and synergies. Additionally, members of the i5K Consortium are involved in the development of tools for visualization and editing of genome annotations (e. g. , Web Apollo; http://gmod. org/wiki/WebApollo), insect genome databases, a... | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Providing a Roadmap for Genome Projects and Arthropod Resources Researchers hoping to exploit genomic data to understand phenotypes, test biological hypotheses, or apply insights for pest control or the preservation of beneficial arthropods require guidance to find relevant sequence and metadata resources. Similarly, g... | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Moving forward, this community-driven list will continue to be populated with contributions from the i5K membership and with regular searches through large, institutional databases such as at the National Center for Biotechnology Information (NCBI; http://www. ncbi. nlm. nih. gov/genome/?term=arthropoda) and the Europe... | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Populating Databases with Genome-Relevant Information for Each Species Taxa with already completed genome sequences have received more extensive postsequencing analysis, curation, and database development than is likely to occur for upcoming genome candidates. At one extreme, sequence and phenotype data for numerous Dr... | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | beetlebase. org), moths (i. e. , the silkworm genome databases SilkDB, www. silkworm. genomics. org. cn/, and KAIKObase, sgp. dna. affrc. go. jp/ KAIKObase/) and pea aphids (AphidBase, www. aphidbase. org) have current sites devoted to incoming genomic and/or biological data. In contrast, limited financial and informat... | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | In addition to the i5K pilot project at Baylor, organizers of independently supported projects involving bees, beetles, and other insects have linked these to the bioproject page, something we would encourage of all arthropod projects as part of NCBI data submission. | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Conclusions Although the i5K initiative is in its early stages, immediate needs have been identified and thus future directions have been drawn. Discussions spurred by the i5K Consortium have led community members to keep abreast of new technologies in sequencing, assembly, and annotation. Training possibilities are im... | 10.1093/jhered/est050 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | ACKNOWLEDGMENTS We wish to thank V. Vallée for fruitful discussion and I. Kerkines for providing us with his data for LiKr dimer. We acknowledge the financial support of the French ANR , Contract No. ANR-09-BLAN-013001. | 10.1063/1.4773019 | article | en | 2,013 | false | false | true | false | Chemistry | https://openalex.org/fields/16 |
cc-by | 44; P = 0. 036) and of 40-44 years (r 2 = 0. 51; P = 0. 019), while it decreased for athletes in the age group of 30-34 years (r 2 = 0. 66, P = 0. 0039). For women, running speed remained stable during the study period for athletes in all age groups. Conclusion: These data suggest that the number of finishers of master... | 10.2147/ijgm.s45265 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | 44; P = 0. 036) and of 40-44 years (r 2 = 0. 51; P = 0. 019), while it decreased for athletes in the age group of 30-34 years (r 2 = 0. 66, P = 0. 0039). For women, running speed remained stable during the study period for athletes in all age groups. Conclusion: These data suggest that the number of finishers of master... | 10.2147/ijgm.s45265 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | 2 Men achieved their fastest running times at the age of 30-49 years. 2 In the Swiss Alpine Marathon, a 78 km mountain ultramarathon held in Davos, Switzerland, an increased age for the fastest running times for both sexes was found. 17 From 1998 to 2011, the age for the fastest running times increased for men from 3... | 10.2147/ijgm.s45265 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Materials and methods All finishes in the Marathon des Sables between 2003 and 2012 were included in an analysis of age, sex, and running speed. The data set for this study was obtained from the website of the race. 18 Data before 2003 were not available. All procedures used in the study met the ethical standards of th... | 10.2147/ijgm.s45265 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Data analysis Due to the changing number of stages and the changes in stage length, running speed was calculated over the whole competition to determine the performance of the athletes. For the analysis of the change in running speed in both sexes and the change in sex difference across years, the results of the annual... | 10.2147/ijgm.s45265 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Statistical analyses were performed using IBM Statistical Package for the Social Sciences Statistics (Version 19, IBM SPSS, Chicago, IL, USA) and GraphPad Prism (Version 5, GraphPad Software, Inc, La Jolla, CA, USA). Significance was accepted at P , 0. 05 (two-sided for t-tests). Data in the text are given as mean ± st... | 10.2147/ijgm.s45265 | article | en | 2,013 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Overall finisher trends Data from 6945 f inishes, including 909 women and 6036 men, have been analyzed from the Marathon des Sables between 2003 and 2012. The number of finishes increased from 37 (2003) to 107 (2012) for women and from 272 (2003) to 687 (2012) for men. Men showed, with a mean number of 603. 6 ± 146. 2 ... | 10.2147/ijgm.s45265 | article | en | 2,013 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | In vivo bioluminescence imaging Mice were inoculated intraperitoneally with luciferin (D-Luciferin potassium salt, Xenogen, California), the luciferase substrate, at a dose of 150 mg/kg before any bioluminescence measurements were made. They were anaesthetized in a 2. 5% isoflurane atmosphere (Aerane, Baxter SA, Maurep... | 10.1371/journal.pntd.0001976 | article | en | 2,013 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments The authors thank Marie-Anne Nicola ( Pasteur Imagopole ) for her help with the IVIS Lumina, Fabrice Chre ´tien and Sandrine Vitry for their expertise and help with the brain analysis and critical discussions and to M. Jones from Transcriptum for English corrections. | 10.1371/journal.pntd.0001976 | article | en | 2,013 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Kelp ecosystems form widespread underwater forests playing a major role in structuring the biodiversity at a regional scale. Some seaweeds such as Laminaria digitata are also economically important, being exploited for their alginate and iodine content. Although some studies have shown that kelp ecosystems are regressi... | 10.1371/journal.pone.0066044 | article | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Occurrence Data of Laminaria digitata Because no comprehensive database of the distribution of L. digitata existed, we compiled occurrence data from different sources to optimize our assessment of the recent distribution of this species. We used data from OBIS (http://www. iobis. org/) and the Ecokelp project (http://w... | 10.1371/journal.pone.0066044 | article | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Large-scale Abiotic Parameters Monthly mean gridded Sea Surface Temperature (SST) data from 1982 to 2009 were taken from NOAA 4 km Advanced Very High Resolution Radiometer (AVHRR) Pathfinder [31] (http:// www. nodc. noaa. gov/SatelliteData/pathfinder4km/). Maximum annual SST was calculated for the Northern European r... | 10.1371/journal.pone.0066044 | article | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | some regions of the eastern English Channel). Data on substratum types were obtained from SHOM database (Service Hydrographique et Oceanographique de la Marine). The diffuse attenuation coefficient for downwards irradiance at 490 nm (Kd490) was used as an indicator of the turbidity. Climatological data were downloaded ... | 10.1371/journal.pone.0066044 | article | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Adequacy between AVHRR data and RCP scenarios. Before using SST data from RCP scenarios, we compared the two datasets for the common period 2006-2009 by applying a normalized Taylor's diagram [43]. The Taylor's diagram (Fig. S3 ) allows assessing the performance of models by summarized in a single diagram the standard... | 10.1371/journal.pone.0066044 | article | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Adequacy between AVHRR Data and RCP Scenarios Since the present distribution of probability of occurrence was based on AVHRR SST data and projections of future probabilities on SST data coming from RCP scenarios (2006-2100), we compared both observed and modeled SSTs for the common period 2006-2009 to prevent any bias... | 10.1371/journal.pone.0066044 | article | en | 2,013 | true | false | false | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Acknowledgments We acknowledge the World Climate Research Programme 's Working Group on Coupled Modeling, which is responsible for CMIP, and we thank the climate modeling groups for producing and making available their model output. For CMIP the U. S. Department of Energy's Program for Climate Model Diagnosis and Int... | 10.1371/journal.pone.0066044 | article | en | 2,013 | false | false | true | false | Earth and Planetary Sciences | https://openalex.org/fields/19 |
cc-by | Sequence Analysis BAC-end sequences were analysed through BLASTN and BLASTX searches against sequence databases. Triticeae-specific repetitive elements were searched against both nucleotide and protein subsets of the Triticeae-specific repeat database (TREP, wheat. pw. usda. gov/ITMI/Repeats/) to identify characterised... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Anchoring of Genetic Markers Genetic markers (Table 1 ) were gathered from published genetic maps contained in the marker repository GrainGenes (wheat. pw. usda. gov) based on their presence on chromosome 1AS. Initial anchoring of physical contigs to maps was performed using standard PCR screening on MTP 3D-pools usin... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Wheat NimbleGen 40 K UniGene Microarray Hybridisation Forty-nine DNA pools were generated from the MTP clones. The MTP was derived from FPC contigs which cover approximately 86% of chromosome arm 1AS (see results and discusison). The DNA pools were used for hybridisation on a custom 126135 K NimbleGen gene expression a... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | 01 for t-tests). Hybridised UniGene probe matches were screened for TEs by Blast against the TREP database. They were also searched against deletion bin-mapped 1AS-specific wheat ESTs [38]. All Blastx searches were performed using a ,1E-10 E-value cut-off. BLASTN searches were run using a 50 nucleotide alignment with.... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Data Deposition The chromosome 1AS BAC library is available through the Centre National de Resources Genomiques Vegetales (CNRGV), Toulouse, France (cnrgv. toulouse. inra. fr/) and the Institute of Experimental Botany, Olomouc, Czech Republic (http:// olomouc. ueb. cas. cz/dna-libraries/cereals). Physical mapping and m... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | e. putative chimeric clones). Many Q-clones causing false connections between clones in Qcontigs were identified to be from the same fingerprint sequencing plate, indicating that well-to-well contamination was a major cause of creating non-linear assembled contigs. Similar problems were previously reported when assembl... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | The 1AS Backbone Consists of LTC BAC Contigs Complemented with FPC Clones The final dataset of 394 LTC BAC contigs represent over 80% of the backbone of the 1AS physical map (Table 3 ). However, these LTC contigs do not include all BACs, which were part of the minimum tiling path produced by FPC. These excluded BACs c... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | The total cumulative size of all BAC contigs is 255. 4 Mbp, corresponding to 92,8% of chromosome 1AS (Table 3 ). In summary, we propose the use of both, LTC and FPC software. LTC overall performed better than FPC on 1AS BAC fingerprints, resulting in larger and more robust contigs. However, we also considered it impor... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | The cumulative length of all BAC end sequences (BES) was 4,704 kb (1. 7% of the 1AS chromosome arm). These sequences allowed the quantitative study of the chromosome arm sequence composition. A total of 180 sequences (2. 6%) had homology with Brachypodium genes, 42 of which had their closest homologs in the 1AS synteni... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | In previous genome surveys of diploid Triticeae species, Copia elements of the Angela/BARE1 family were clearly the most abundant with more than 10-12% of the whole genomic sequence [48] [49] [50] [51] [52]. In contrast, in our dataset, Angela/ BARE1 only contributed 4. 16% to the BES. This could indicate that the TE ... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Evaluation of the Chromosome 1AS Pm3 locus using Physical Contigs and Sequenced BACs To investigate the validity of the physical map at a smaller scale, we used the previously published sequence covering the Pm3 locus (accession number AY146587 [59] ). This sequence has a size of approximately 178 kb and was isolated ... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | A total of 12 UniGene hybridisation probes were found on the two physical contigs. These could be assigned to two gene loci. Three of the probes correspond to the Pm3 gene while a second cluster of 7 probes corresponds to one or more glutenin genes (Figure 6 ). This is in agreement with previous studies, which reporte... | 10.1371/journal.pone.0080272 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Acknowledgments We are grateful to Prof. B. S. Gill ( Kansas State University, Manhattan, USA ) for seeds of the double ditelosomic line 1A of wheat T. aestivum L. cv. Chinese Spring. We thank Jan Buchmann , Christopher Middleton , Simone Oberhaensli and Stefan Roffler for their competent support in computer pr... | 10.1371/journal.pone.0080272 | article | en | 2,013 | false | false | true | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | SAXS (small-angle X-ray scattering) SAXS data using purified TorD His , TorA/TorD His and TorA SP /TorD His samples were recorded on the ID14-3 BioSAXS beamline at the European Synchrotron Radiation Facility (ESRF Grenoble, France). Sample-detector distance was 2. 43 m for an X-ray wavelength of 0. 931 Å (1 Å = 0. 1 nm... | 10.1042/bj20121832 | article | en | 2,013 | true | true | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Solvent contributions (buffer backgrounds collected before and after every protein sample) were averaged and subtracted from the associated protein sample using the program PRIMUS [29]. | 10.1042/bj20121832 | article | en | 2,013 | false | true | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | CD analysis CD analysis of protein samples was provided as a service by the Scottish Circular Dichroism Facility, University of Glasgow. Spectra of protein solutions were obtained using a JASCO J-810 spectropolarimeter. Far-UV CD measurements (185-260 nm) were collected in quartz cells of 0. 02 cm pathlength at 25 • C ... | 10.1042/bj20121832 | article | en | 2,013 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | 0 s; and data pitch, 0. 2 nm. Protein was analysed in appropriate buffer (50 mM Tris/HCl, pH 7. 5, 200 mM KCl and 1 mM DTT). The spectra were corrected for protein concentration and cell pathlength before being analysed by DichroWeb (http://dichroweb. cryst. bbk. ac. uk), an online server which hosts the various algori... | 10.1042/bj20121832 | article | en | 2,013 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | 5), 200 mM KCl and 1 mM DTT at 0. 5 ml/min; Shimadzu LC Solutions software was used to control the HPLC, and Astra V software was used for the HELEOS-II and rEX detectors. BSA was used for molecular mass calibration. The Astra data collection was 1 min shorter than the LC solutions run to maintain synchronization. Blan... | 10.1042/bj20121832 | article | en | 2,013 | true | true | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | To investigate this further, a construct was designed that would co-overproduce TorD His together with the first 675 amino acids of TorA, including the signal peptide (designated TorA CT ). First, the construct was expressed in a strain lacking endogenous TMAO reductase activity under strict anaerobic conditions, which... | 10.1042/bj20121832 | article | en | 2,013 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | A low-resolution model of TorD His , obtained using the program DAMMIN [37] , is shown in Figure 7(A). The overall shape of TorD His is compact and elliptical with dimensions of approximately 36 Å×50 Å×34 Å. From the crystal structures of S. massilia TorD [17] and the TorD family protein DmsD (A) Two different vi... | 10.1042/bj20121832 | article | en | 2,013 | true | true | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Further evidence for overlapping TorD-binding sites near the Nterminus of TorA come from analysis of the SAXS data obtained in the present study. To investigate the possible arrangement of TorA and TorD in the TorAD complex, rigid body modelling was undertaken using the SASREF program [47] and the structures of S. ma... | 10.1042/bj20121832 | article | en | 2,013 | false | true | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | ACKNOWLEDGEMENTS We acknowledge Dr Bérengère Ize , Dr Grant Buchanan and Dr Dave Guymer for useful discussions and preliminary studies. We also thank Dr Cyril Dian for technical assistance at the ID14-3 BioSAXS beamline at the ESRF. | 10.1042/bj20121832 | article | en | 2,013 | false | false | true | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Gene set enrichment analysis of the T cell transcriptome In order to further investigate the biological basis of the gene expression differences observed between T cells from psoriasis patients and controls, we analyzed the global transcriptome using the GSEA approach [30, 31]. A total of 55 gene sets were found to be... | 10.1186/1471-2164-14-825 | article | en | 2,013 | true | false | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Microarray analysis and differential gene expression determination Whole genome gene expression analysis of in vitro activated T cells for 17 psoriasis patients and 7 healthy controls was carried out by the HudsonAlpha Institute for Biotechnology using the Illumina HT-12 V4 microarrays (Illumina, USA). Briefly, the Ill... | 10.1186/1471-2164-14-825 | article | en | 2,013 | true | true | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Sample labeling, microarray hybridization, scanning and raw data acquisition were performed at HudsohAlpha Institute for Biotechnology (Alabama, USA). Data preprocessing, normalization and differential expression was performed using the R-statistical software v12. 0 [63]. Log2-transformed data was normalized using the... | 10.1186/1471-2164-14-825 | article | en | 2,013 | true | true | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | In order to contextualize the gene networks associated with psoriasis, we performed a comprehensive search for previous studies having found evidence between the network genes and the disease. Using each gene annotation and the term 'psoriasis' we performed systematic queries using NCBI Pubmed database (October 2013). ... | 10.1186/1471-2164-14-825 | article | en | 2,013 | true | false | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Functional annotation of expression modules Gene Ontology (GO) category enrichment analysis was performed using DAVID online tool (i. e. Database for Annotation, Visualization and Integrated Discovery, http://david. abcc. ncifcrf. gov/, Bethesda, MD, USA) [29]. Briefly, GO enrichment is calculated comparing the freque... | 10.1186/1471-2164-14-825 | article | en | 2,013 | true | true | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Gene set enrichment analysis GSEA is a software method to analyze and interpret microarray data using biological knowledge [30, 31]. Using the differential gene expression result between cases and controls and a list of predefined biological sets of genes, GSEA tests the overrepresentation each gene set in the group o... | 10.1186/1471-2164-14-825 | article | en | 2,013 | true | false | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Acknowledgements We thank Devin Absher and the HudsonAlpha Institute for Biotechnology for their support. We thank Natalia Figueras Sanchez for technical assistance. This study was funded by of the Spanish Ministry of Economy and Competitiveness , grant numbers: PSE-010000-2006-6 and IPT-010000-2010-36. The s... | 10.1186/1471-2164-14-825 | article | en | 2,013 | false | false | true | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Availability of supporting data The datasets supporting the results of this article are available in NCBI's gene Expression Omnibus and are accessible through GEO Series accession number GSE47598 (http://www. ncbi. nlm. nih. gov/geo/query/acc. cgi?acc=GSE47598). | 10.1186/1471-2164-14-825 | article | en | 2,013 | true | false | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | The Workshop covered a range of topics but provided a facility to focus on some key areas of research. Highlights from this included the following: • The events of late July into early August 2010 involved the first major active region complex of the new solar cycle, releasing at least four coronal mass ejections on 01... | 10.1007/s11207-013-0267-y | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | He became a Co-Investigator on the Heliospheric Imager (HI) instruments onboard the twin NASA STEREO spacecraft and also a Member of the Solar and Space Weather Key Science Project for LOFAR. During 2007 and 2008 he was one of the leading organisers for the International Heliophysical Year. Andy also achieved his final... | 10.1007/s11207-013-0267-y | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Acknowledgements It is a pleasure to acknowledge the Editors and staff at Solar Physics for their work in pulling this Topical Issue (TI) together, and thanks again to the team of referees for their time and effort in making this a very successful TI, covering a broad range of topics and studies related to observing ... | 10.1007/s11207-013-0267-y | article | en | 2,013 | false | false | true | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Note that this description involves only the data part. In the imperfect CSI case, we consider that channel estimates are obtained via the use of pilot symbols, and the description will be refined accordingly. These details are provided in the next sections. We study two relaying protocols: DMF relaying and DF relaying... | 10.1186/1687-6180-2013-170 | article | en | 2,013 | true | false | false | false | Computer Science | https://openalex.org/fields/17 |
cc-by | Imperfect CSI As far as the imperfect CSI case is concerned, we restrict our attention to only block fading channels with F blocks and quasi-static fading with F = 1. The reason is that channel estimation is assumed to be obtained via a pilotbased approach for [23] , which is clearly not compatible with fully interlea... | 10.1186/1687-6180-2013-170 | article | en | 2,013 | true | false | false | false | Computer Science | https://openalex.org/fields/17 |
cc-by | Acknowledgements The research work is supported in part by the European Commission under the auspices of the FP7-PEOPLE MITN-CROSS FIRE project (Grant 317126 ) and in part by the European Commission in the framework of the FP7 Network of Excellence in Wireless COMmunications NEWCOM# (Grant agreement no. 3183... | 10.1186/1687-6180-2013-170 | article | en | 2,013 | false | false | true | false | Computer Science | https://openalex.org/fields/17 |
cc-by | 2 ). To elucidate primary effects of the wat1 mutation, we carried out comparative microarray analysis profiling mRNA global expression of 10-day-old in vitro-grown wild-type and wat1-1-mutant seedlings. This revealed 40 genes that exhibited significantly altered expression levels in wat1-1, with 13 genes displaying lo... | 10.1038/ncomms3625 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | S1 ). These results revealed that the wat1-1 mutation modulates the expression levels of several auxin-responsive genes. Finally, to determine whether there is a causal link between lower auxin content and the fibre phenotype in wat1 stems 19 , we locally applied auxin (2,4-D and NAA) to growing stem segments of wat1... | 10.1038/ncomms3625 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Finally, WAT1 did not facilitate 3 H-Trp uptake (Fig. 6e ). WAT1-mediated IAA influx was further confirmed by testing it in an independent heterologous expression system: Xenopus oocytes (Fig. 6f ). As compared with water-injected oocytes, WAT1 complementary ribonucleic acid (cRNA)-injected oocytes incorporated signifi... | 10.1038/ncomms3625 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | To our knowledge, this is the first report demonstrating a role for the vacuole in the regulation of auxin homoeostasis. WAT1mediated auxin transport across the tonoplast membrane uncovers an additional level of intracellular complexity in auxin signalling pathways in plant cells. Paradoxically, the presence of auxin a... | 10.1038/ncomms3625 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | 0) and 1 mM each of potassium ferri/ferrocyanide. After the reaction, the GUS staining was fixed for 1 h in 3% paraformaldehyde, 0. 5% glutaraldehyde and 50 mM sodium phosphate buffer (pH 7. 0). Microarray analysis. Microarray analysis was performed with a 22-k CATMA Arabidopsis array. Two independent biological compar... | 10.1038/ncomms3625 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | urgv. evry. inra. fr/CATdb/) under the project name 'RS06-04_Noduline-like'. Coexpression analysis was performed using the Arabidopsis Coexpression Data Mining Tools from the University of Leeds 21. WAT1 cDNA cloning for expression in yeast. WAT1 cDNA amplified from 10-day-old in vitro Arabidopsis Col-0 plantlets usin... | 10.1038/ncomms3625 | article | en | 2,013 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Acknowledgements We are grateful to R. Daniel Gietz ( University of Manitoba ) for advice on yeast selection and to Wolfgang Koch ( Tu ¨bingen Universita ¨t ) for the gift of the pDR196 vector. We also thank Barbara Bassin , Rita Saraiva and Maja Schellenberg ( Universita ¨t Zu ¨rich ) for their help in perfor... | 10.1038/ncomms3625 | article | en | 2,013 | true | false | true | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | R. and D. G. , and ANR-06-GPLA-012 ' Genoplante Transportome ' to C. C. -F. , B. L. and J. -B. T. O. D. and W. B. acknowledge funding from the Stanford University GCEP (Towards New Degradable Lignin Types) and K. M. acknowledges the Ghent University BOF Grant 174PZA05. K. L. and O. N. acknowledge the Swedish Go... | 10.1038/ncomms3625 | article | en | 2,013 | false | false | true | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Hydrological signatures The variations of three hydrological signatures (runoff coefficient, precipitation elasticity of streamflow and first/last deciles of streamflow) are studied at sub-basin scale using 16 ORE HYBAM gauge stations (table 1) to characterize the Table 1. List of ORE HYBAM gauge stations over the Ama... | 10.1088/1748-9326/8/1/014035 | article | en | 2,013 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | A high (low) runoff coefficient points out that a large amount of water leaves the basin as streamflow (as ET). R coeff = Q P. (3) Three runoff coefficients are computed for each subbasin: • R OBS coeff is computed from ORE HYBAM discharge data (except for the river mouth discharge whose value is from Callede et al (2... | 10.1088/1748-9326/8/1/014035 | article | en | 2,013 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | By computing elasticity coefficients, we also show that the southern basins with low runoff coefficient are generally more responsive to precipitation change than the western basins with a high runoff coefficient. This result is in agreement with the findings by Chiew (2006) and Paiva et al (2011a). Some uncertainti... | 10.1088/1748-9326/8/1/014035 | article | en | 2,013 | false | true | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Acknowledgments This work was financially supported by the GIS ( Groupement d'Intérêt Scientifique) REGYNA (REGioNAlisation des précipitations et impacts hYdrologiques et agronomiques ) and the EU-FP7 AMAZALERT (Raising the alert about critical feedbacks between climate and long-term land-use change in the Amazon) ... | 10.1088/1748-9326/8/1/014035 | article | en | 2,013 | false | false | true | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Numerical analysis Numerical temporal traces of the different neurons (Figs. 1, 6, 8, 9) were reproduced by implementing in MATLAB (available at http://www. mathworks. com) the original models as described in the associated papers. The phase portraits in Figures 1 and 3 were hand-drawn using the Open Source vector ... | 10.1371/journal.pcbi.1003040 | article | en | 2,013 | false | true | false | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | Acknowledgments The reviewers are gratefully acknowledged for insightful comments and suggestions about the original version of the manuscript. | 10.1371/journal.pcbi.1003040 | article | en | 2,013 | false | false | true | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | The Large Yield Radiometer (LYRA) is an XUV-EUV-MUV (soft X-ray to mid-ultraviolet) solar radiometer onboard the European Space Agency PROBA2 mission that was launched in November 2009. LYRA acquires solar irradiance measurements at a high cadence (nominally 20 Hz) in four broad spectral channels, from soft X-ray to MU... | 10.1007/s11207-013-0252-5 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Section 3 provides an overview of the data products that are distributed to the scientific community and details their calibration. Section 4 briefly presents the main fields of scientific research currently investigated, exploiting LYRA data. | 10.1007/s11207-013-0252-5 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Voltages are then switched to frequencies by a voltage-to-frequency converter (VFC). Regularly, those VFC are connected to stable calibrated onboard voltage sources of 5 volts, 2. 5 volts, and 0 volts. These measurements are interleaved in the telemetry, to be used as reference when processing the data. At the end of t... | 10.1007/s11207-013-0252-5 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Data Products LYRA produces time series of spectral irradiance in its four bandpasses in a quasi-uninterrupted way. Interruptions might happen during calibration campaigns (once every two weeks on average), or during the Winter occultation season, when the spacecraft transits the Earth's shadow. Nevertheless, since PRO... | 10.1007/s11207-013-0252-5 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | oma. be). They consist of uncalibrated and calibrated data, as well as various quicklook datasets (see Table 2 ). Once downloaded, raw LYRA data in counts are converted back to counts ms -1 , dividing them by the integration time, and distributed without further processing through the instrument website as level-1/eng... | 10.1007/s11207-013-0252-5 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Therefore, only later data points, representing calibration campaigns after 23 June 2010, are used to estimate the future development. The loss caused by degradation is estimated relative to the first light individually for each channel. The degradation is corrected by adding this loss to the current levels. Correction... | 10.1007/s11207-013-0252-5 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | This out-of-band radiation constitutes a source of measurement error that might be important in the case of the Lyman-α channel (see purities in Table 1 ). Since LYRA channels cover broad spectral ranges, it is not possible to directly invert Equation (1) to retrieve the spectral irradiance [E(λ, t)] from the measured... | 10.1007/s11207-013-0252-5 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Non-Solar Features in the LYRA Data Undesired features, such as imprints of large angle rotations of the spacecraft or perturbations due to the South Atlantic Anomaly (SAA), are present in LYRA data and must not be interpreted as solar signal variation. This section provides a list of those features. | 10.1007/s11207-013-0252-5 | article | en | 2,013 | true | false | false | false | Physics and Astronomy | https://openalex.org/fields/31 |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.