title stringlengths 1 1.19k | keywords stringlengths 0 668 | concept stringlengths 0 909 | paragraph stringlengths 0 61.8k | PMID stringlengths 10 11 |
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3. Results | PMC10454381 | |||
3.1. Baseline Characteristics | Demographic and ANBQ chewing history characteristics are presented in The following variables were compared between randomization groups: (a) age, (b) sex, (c) race/nationality, (d) study site, (e) participant group size, (f) number of ANBQ chews per day, (g) BQDS score, and (h) education level. The results revealed se... | PMC10454381 | ||
3.2. Cessation Outcomes | REGRESSION | The current analyses included 88 participants in the intervention group and 88 participants in the control group. All participants included in these analyses provided outcome data at the 22-day follow-up. Although we aimed to schedule the initial follow-up assessments exactly 22 days following baseline, scheduling and ... | PMC10454381 | |
4. Discussion | EVENTS, RECRUITMENT, RECRUITMENT | This analysis of BENIT outcomes revealed strong self-reported intervention effects at the 22-day post-baseline assessment. Participant self-reports of 38.6% cessation for those in the intervention condition versus 9.1% cessation for those in the control condition met the Stage 3 criteria for early termination of the tr... | PMC10454381 | |
Author Contributions | Conceptualization; funding acquisition; supervision; writing—original draft, T.A.H. Formal analysis, L.R.W. and G.B. Data curation; resources, A.J.P.M. Project administration; investigation, A.A.F. Project administration; investigation, P.P. Data curation, J.S.N.C., L.F.T. and P.P.S. Project administration; resources, ... | PMC10454381 | ||
Institutional Review Board Statement | MAY | The study was conducted in accordance with the Declaration of Helsinki, and approved by the Institutional Review Board of the University of Guam (#16-04, approved 13 May 2016). The University of Guam IRB monitored the study for both study locations (Guam and Saipan) because Saipan does not have an IRB suitable for moni... | PMC10454381 | |
Informed Consent Statement | Informed consent was obtained from all subjects involved in the study, and all participants were compensated for their time. | PMC10454381 | ||
Data Availability Statement | Cancer | CANCER | Data will be shared pursuant to the data and specimen sharing policies and procedures of the Pacific Island Partnership for Cancer Health Equity (PIPCHE). Contact information: Hali R. Robinette at the University of Hawaii Cancer Center ( | PMC10454381 |
Conflicts of Interest | The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analysis, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results. | PMC10454381 | ||
References | ORAL CANCER | CONSORT flow diagram for the Betel Nut Intervention Trial.Description of the intervention sessions for the Betel Nut Intervention Trial.Introduction to the program. Obtain signed, informed consent.Health risks of areca nut and betel quid (ANBQ) chewingIntroduce self-monitoring logsIntroduce ‘trigger’ logsDiscuss how to... | PMC10454381 | |
Background | pain, OLP | CAVITY, ORAL LICHEN PLANUS, CHRONIC INFLAMMATORY DISEASE | Oral lichen planus (OLP) is a chronic inflammatory disease of the oral cavity that affects many patients’ daily living activities. Topical corticosteroids are the first-line drug for treating OLP. The Oral Impact on Daily Performances index (OIDP) is an Oral-Health-Related Quality of Life (OHRQoL) measure developed to ... | PMC10662109 |
Methods | NRS, OLP | Seventy-two OLP patients were treated by topical corticosteroids based on their dentists’ clinical judgments. Clinical and patient-based outcomes were assessed at baseline and follow-up visit. The clinical outcomes were evaluated by the highest and total Thongprasom sign score. Patient-based outcomes were evaluated by ... | PMC10662109 | |
Results | This study comprised 59 women and 13 men. All clinical and patient-based outcomes were significantly reduced after 1-month treatment with topical corticosteroids ( | PMC10662109 | ||
Conclusions | pain, OLP | Topical corticosteroids were significantly effective after 1-month treatment of OLP based on the clinical and patient-based outcomes. The OHRQoL improvement was significantly related to the reductions in pain and clinical severity. | PMC10662109 | |
Trial registration | TCTR | The trial was registered at the Thai Clinical Trials Registry (TCTR identifier: TCTR 20221110001). | PMC10662109 | |
Keywords | PMC10662109 | |||
Background | pain, OLP | CAVITY, ORAL LICHEN PLANUS, CHRONIC INFLAMMATORY DISEASE | Oral lichen planus (OLP) is a common chronic inflammatory disease of the oral cavity that affects the daily living activities in many patients. Most OLP patients are middle-aged women; the most common lesion sites are the buccal mucosa followed by the tongue, and gingiva [Currently, OLP clinical trials should use a tri... | PMC10662109 |
Methods | PMC10662109 | |||
Study subjects | OLP | The sample size was calculated using 80% power and 95% confidence level according to data from McGrath et al. [The patients were recruited from the Oral Medicine clinic, Faculty of Dentistry, Chulalongkorn University. The inclusion criteria were being over 18 years old, patients diagnosed with OLP or compatible with OL... | PMC10662109 | |
Data collection | PMC10662109 | |||
Demographic data | palate, OLP | Age, sex, systemic condition, medication taking and OLP duration since the first diagnosis were collected from the history taking at the first visit. In addition, the OLP locations (e.g. lips, labial mucosa, buccal mucosa, gingiva, tongue, floor of the mouth, and palate), experience with OLP treatment (i.e., new or fol... | PMC10662109 | |
Clinical outcomes | palate, OLP lesions, OLP | ATROPHIC | Regarding the clinical data collection, the highest Thongprasom sign score was based on the original Thongprasom sign scoring system, i.e., “0” indicated no lesions; “1” indicated white striae only, “2” indicated white striae with an atrophic area of less than 1 cmAs mentioned earlier that Thongprasom sign scoring syst... | PMC10662109 |
Patient-based outcomes | pain, OLP | ULCERATION | The patients were asked about the chief symptoms of OLP that made them visit the dentist at baseline, such as a burning sensation, ulceration, and roughness. The patients could have more than 1 symptom or have no symptoms. In the aspect of pain, the OLP patients reported their NRS score for their worst pain during 1 mo... | PMC10662109 |
Data analysis | pain | Statistical calculations were performed with SPSS software (SPSS 22.0 for Windows; SPSS, Chicago, IL, USA). Descriptive statistics were used for the demographic and clinical data. The Kolmogorov-Smirnov normality tests were performed to determine the normal data distribution in each outcome. The pain score and total Th... | PMC10662109 | |
Results | PMC10662109 | |||
OLP patient characteristics | Diabetes mellitus- Thyroid disease-, thyroid disease, Anti-dyslipidemia, Lips- Labial mucosa- Buccal mucosa-, palate, diabetic, hypertensive, OLP, gout, hypertension, dyslipidemia | THYROID DISEASE, DYSLIPIDEMIA, DIABETES MELLITUS, CAVITY, HYPERTENSION, FLOOR OF MOUTH, ULCERATION, GOUT, HYPERTENSION, DISEASES, DYSLIPIDEMIA | The 72 patients (100% response rate) in this study consisted of 59 women (81.9%) and 13 men (18.1%). The mean age of the patients in this study was 53.3 ± 12.4 years old (range 22–81 years old). Forty-six patients (63.9%) were between 30 and 60 years old. Twenty-three patients (31.9%) were more than 60 years old and 3 ... | PMC10662109 |
Discussion | gingiva, palate, pain, OLP | MINOR, CAVITY | Nowadays, the importance of utilizing patient-based outcomes alongside clinical outcomes has been recognized. The current study’s findings could support patient-centered care in evidence-based dentistry in relation to OLP treatment with topical corticosteroid. Most of the patients participating in this study benefited ... | PMC10662109 |
Conclusion | pain, OLP | After a 1-month OLP treatment with topical corticosteroids, the highest Thongprasom sign score, total Thongprasom sign score, and pain score were significantly reduced. The patients’ overall OHRQoL and daily performances were significantly improved, except for Relaxing including sleeping of which none of the patients r... | PMC10662109 | |
Acknowledgements | ORAL DISEASE | The authors thank the participants in this study. We appreciate the staff of the Department of Oral Medicine and the Research Unit in Oral Diseases, Faculty of Dentistry, Chulalongkorn University for their assistance. We also express our thanks to Dr. Kevin Tompkins for English language editing. | PMC10662109 | |
Authors’ contributions | PP, SK contributed to the study conception and design, methodology, project administration, supervision, and writing-review & re-editing. WK contributed to the data collection, formal analysis and interpretation of the data, and writing-original draft. PP contributed in the funding acquisition. All authors read and app... | PMC10662109 | ||
Funding | This research was supported by the Faculty Research grant (DRF 65043), Faculty of Dentistry, Chulalongkorn University and the Royal College of Dental Surgeons of Thailand grant. | PMC10662109 | ||
Data availability | All data generated or analyzed during this study are included in this published article. | PMC10662109 | ||
Declarations | PMC10662109 | |||
Ethics approval and consent to participate | The present study was performed with informed consent following protocols approved by the Human Research Ethics Committee of the Faculty of Dentistry, Chulalongkorn University (certificate number HREC-DCU 2021-090 approved on 3 December 2021). All methods were performed in accordance with the Declaration of Helsinki. | PMC10662109 | ||
Consent for publication | Not applicable. | PMC10662109 | ||
Competing interests | The authors declare no competing interests. | PMC10662109 | ||
References | PMC10662109 | |||
Background and purpose | both-bone forearm fractures, loss of flexion and extension of the elbow | previous RCT compared short-term results of above-elbow cast (AEC) with early conversion to below-elbow cast (BEC) in children with non-reduced diaphyseal both-bone forearm fractures. After 7 months both groups had comparable function. Our primary aim was to investigate whether forearm rotation improves or worsens over... | PMC10561100 | |
Patients and methods | We performed long-term follow-up (FU) of a previous RCT. All patients were invited again for the long-term FU measurements. Primary outcome was limitation of forearm rotation. Secondary outcomes were loss of flexion and extension of the elbow and wrist compared with the contralateral forearm, the ABILHAND-Kids question... | PMC10561100 | ||
Results | The mean FU was 7.5 (4.4–9.6) years. Of the initial 47 children, 38 (81%) participated. Rotation improved in both groups over time, with no significant difference in the final forearm rotation: 8° (SD 22) for the AEC group and 8° (SD 15) for the BEC group with a mean difference of 0° (95% confidence interval –13 to 12)... | PMC10561100 | ||
Conclusion | both-bone forearm fracture, fractures | Long-term follow-up showed that loss of forearm rotation after a non-reduced diaphyseal both-bone forearm fracture improved significantly compared with that at 7 months, independent of the initial treatment and children aged < 9 will have almost full recovery of function. This substantiates that the remaining growth be... | PMC10561100 | |
Patients and methods | fracture | Our study is a long-term follow-up of a previous RCT. All 47 patients who had been previously included in the RCT by Colaris et al. [Criteria for reduction of the fracture of radius and/or ulna based on anteroposterior and/or lateral radiographs | PMC10561100 | |
Outcome measures | angulation | SECONDARY, COMPLICATION | Our primary outcome was change in loss of forearm rotation in comparison with the contralateral side and a comparison between the 2 casting methods. Secondary outcome measures were change in loss of flexion and extension of the elbow and wrist compared with the contralateral forearm, the Dutch version of the DASH and A... | PMC10561100 |
Statistics | pronation, supination | SECONDARY | We compared the baseline characteristics and functional outcome at 7 months and at 7.5 years between the included patients and those lost to follow-up to rule out potential effects of attrition. Long-term results of primary and secondary outcomes were compared between the 2 groups (AEC vs. BEC) using independent T-test... | PMC10561100 |
Ethics, registration, consent, funding, and disclosures | For this post-trial follow-up study ethics approval was again obtained from the regional medical ethical committee (Dnr NL41839.098.12). The original RCT was registered in | PMC10561100 | ||
Results | angulation, loss of flexion–extension of the elbow, fractures, coronal radial angulation, mean loss of forearm rotation, ’ loss of rotation to 7 months (P = 0.02) | SECONDARY | 38 of 47 patients (17 of 23 with AEC and 21 of 24 with BEC) responded to the follow-up invitation and were clinically and radiologically assessed for long-term follow-up. The mean length of follow-up was 7.5 (4.4–9.6) years. Baseline characteristics were similar between the 2 groups (Baseline characteristics of the pop... | PMC10561100 |
Discussion | diaphyseal both-bone forearm fractures, fracture, angulation, coronal radial angulation, loss of flexion and extension of the elbow | SECONDARY | Our primary aim was to investigate whether forearm rotation improves or worsens over time. We showed that rotation improved in both groups over time, with no significant difference in the final forearm rotation: 8° (SD 22) for the AEC group and 8° (SD 15) for the BEC group with a mean difference of 0 (CI –13 to 12). Ea... | PMC10561100 |
Previous research | fractures, both-bone forearm fractures, re-fracture, malunion | A systematic search of the literature on previous long-term follow-up studies for pediatric forearm fractures resulted in less than 15 studies in total, and none of them was an RCT. Most studies were published more than 5 years ago, and half of the studies were case reports. Therefore, our prospective randomized multi-... | PMC10561100 | |
Study limitations | intramedullary nailing [The primary limitation, malunion | Overall, this is a relatively favorable group, as they have little displacement with no need for reduction and, with that, they have little risk of malunion and rotation limitation. This contrasts with dislocated cases that often re-dislocate causing malunion or rotation problems, resulting in more intramedullary naili... | PMC10561100 | |
1. Introduction | ± | Aspartate supplementation has been reported to improve endurance performance by facilitating the tricarboxylic acid cycle flux. The present study was performed to investigate the effects of aspartate supplementation on repeated-sprint performance and blood pH. Following an overnight fast, fourteen healthy males complet... | PMC10746991 | |
2. Materials and Methods | PMC10746991 | |||
2.1. Study Design and Participants | RECRUITMENT, DISEASES | The present study was a randomized, double-blind, placebo-controlled, crossover study. A total of 15 healthy active males were recruited in the present study. Fourteen participants completed all experiments (means ± standard errors; age 22.3 ± 0.3 years, height 172.1 ± 1.7 cm, weight 65.4 ± 2.2 kg), while a participant... | PMC10746991 | |
2.3. Measurements | PMC10746991 | |||
2.3.1. Repeated-Sprint Performance | MP, fatigue | During each sprint, the peak cadence and peak and mean power outputs were recorded. The fatigue index (FI) was calculated as a percentage reduction of mean power output from the best (MP | PMC10746991 | |
2.3.2. Perceptual Response | The perceived exertions for breathing (RPE | PMC10746991 | ||
2.3.3. Cardiorespiratory Data | The HR was measured and recorded every second during the repeated-sprint exercise using a HR sensor (H2; Polar Electro Oy., Kempele, Finland) and a wireless monitor (RCX5; Polar Electro Oy., Kempele, Finland), and the mean HR was calculated for each exercise set. The HR sensor was attached with an elastic band on the c... | PMC10746991 | ||
2.3.4. Blood Sampling and Analysis | BLOOD, SEPARATION | Blood samples were collected eight times through each trial (at baseline, immediately before and after each exercise set, and 60 min after exercise completion) into 15 mL and 2.5 mL syringes via a cannula inserted into the antecubital vein. Immediately after blood sampling, 12 mL of blood was transferred into an ethyle... | PMC10746991 | |
2.3.5. Statistical Analysis | ± | All data are presented as means ± standard errors, and 95% confidence intervals (CI) are also presented. The test–retest reliability for peak cadence and peak and mean power outputs during the first 6 s maximal sprint was calculated. The intraclass correlation coefficients were 0.96, 0.99, and 0.93, respectively; the c... | PMC10746991 | |
3. Results | PMC10746991 | |||
3.1. Cadence, Peak, and Mean Power Outputs during Repeated-Sprint Exercise | The changes in peak cadence during each exercise set of the repeated-sprint exercise are shown in | PMC10746991 | ||
3.2. FI, RPE, and Cardiorespiratory Data during Repeated-Sprint Exercise | There were no significant differences between trials in FI (ASP, 22.1% ± 1.7% [CI: 18.8–25.5%]; PLA, 20.9% ± 1.9% [CI: 17.1–24.7%]), RPE | PMC10746991 | ||
3.3. Blood pH, Bicarbonate, and Base Excess Levels during Exercise | The pH at baseline was 7.407 ± 0.006 [CI: 7.395–7.419] in the ASP and 7.406 ± 0.004 [CI: 7.398–7.414] in the PLA trials, and these levels were immediately reduced after repeated-sprint exercise. The ASP trial showed a trend for higher blood pH than the PLA trial ( | PMC10746991 | ||
3.4. Blood Parameters and Plasma Amino Acid Profile | No significant difference between the ASP and PLA trials was observed in the mean blood lactate (13.1 ± 1.1 and 13.3 ± 1.0 mmol/L) or glucose concentrations (103 ± 5 and 104 ± 5 mg/dL). Also, no significant difference was observed in the plasma adrenaline, noradrenaline, dopamine, and serum albumin concentrations (Comp... | PMC10746991 | ||
4. Discussion | reduction in blood pH | In the present study, we examined the effects of aspartate supplementation on performance and blood pH during repeated-sprint exercise in healthy males. Consequently, the ASP trial evidenced a smaller reduction in blood pH and increased peak cadence during the second exercise set than the PLA trial. To the best of our ... | PMC10746991 | |
5. Conclusions | Aspartate supplementation improved peak cadence during the second exercise set of repeated-sprint exercise compared with placebo supplementation. However, the performance-enhancing effect was limited, since no significant effect was observed for the mean power output throughout the exercise session. Aspartate is natura... | PMC10746991 | ||
Author Contributions | N.H. | K.Y., M.O., T.K., W.S., F.T., Y.A. and K.G. designed research. K.Y., N.H. and D.S. conducted experiments. W.S. and F.T. analyzed data. K.Y. drafted the manuscript. W.S., Y.A. and K.G. revised the manuscript. All authors have read and agreed to the published version of the manuscript. | PMC10746991 | |
Institutional Review Board Statement | This study was conducted in accordance with the Declaration of Helsinki. All participants were given an overview of the study and provided written informed consent. The study was approved by the Research Ethics Committees of Ritsumeikan University, Japan (BKC-IRB-2018-071, approved on 3 June 2019), and Ajinomoto Co. In... | PMC10746991 | ||
Data Availability Statement | Data are contained within the article. | PMC10746991 | ||
Conflicts of Interest | The test foods were provided by Ajinomoto Co., Inc. M.O., T.K., W.S., F.T. and Y.A. are employed by the company Ajinomoto Co., Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. | PMC10746991 | ||
References | BLOOD | Experimental overview.Changes in peak cadence during repeated-sprint exercise with aspartate (ASP) and placebo supplementations (PLA). Mean ± SE. * Blood pH (Cardiorespiratory responses during repeated-sprint exercise with aspartate (ASP) and placebo supplementations (PLA).Mean ± SE. HR: heart rate. Blood lactate, gluc... | PMC10746991 | |
Background | METASTASIS | The detection of peritoneal metastasis (PM) is limited by current imaging tools. In this prospective study, we aimed to evaluate the sensitivity and specificity of peritoneal cell-free DNA (cfDNA) for diagnosis of PM. | PMC10114319 | |
Methods | tumor, Colorectal cancer, CRC | TUMOR, COLORECTAL CANCER | Colorectal cancer (CRC) patients with/without PM were enrolled. The cfDNA experimental personnel and statists were blinded to the diagnosis of PM. Ultradeep sequencing covering large genomic regions (35000X, Next-generation sequencing) of cfDNA in peritoneal lavage fluid (FLD) and matched tumor tissues was performed. | PMC10114319 |
Results | A total of 64 cases were recruited prospectively and 51 were enrolled into final analysis. In training cohort, 100% (17/17) PM patients obtained positive FLD cfDNA, comparing to 5/23 (21.7%) in patients without PM. Peritoneal cfDNA had a high sensitivity of 100% and specificity of 77.3% for diagnosis of PM (AUC: 0.95).... | PMC10114319 | ||
Supplementary Information | The online version contains supplementary material available at 10.1186/s13148-023-01479-9. | PMC10114319 | ||
Keywords | PMC10114319 | |||
Background | peritoneal ascites, cancers, CRC, deaths, Colorectal cancer | CANCERS, COLORECTAL CANCER | Colorectal cancer (CRC) is one of the most common cancers worldwide, ranking the third position of new cases and deaths of all cancers [Preliminary study has suggested the feasibility of cfDNA detection in peritoneal ascites and peritoneal lavage fluid (FLD) [ | PMC10114319 |
Results | PMC10114319 | |||
Clinicopathological characteristics | CRC | A total of 64 consecutive patients with CRC were recruited prospectively. Thirteen cases were excluded for subsequent analysis, due to insufficient tissue or peritoneal lavage for NGS assays (The workflow of patient enrollment in this study. 40 CRC patients were including in the training cohort underwent NGS assays for... | PMC10114319 | |
The concordance of gene mutations in peritoneal lavages and tumor tissues | tumor | MUTANT, TUMOR | The sequencing depth and DNA insert sizes fulfilled the quality of control in both FLD and TIS of PM and non-PM (Additional file The mutational profiling of cfDNA in matched FLD and TIS of PM and non-PM patients were compared by cluster plot. More mutations were shown in both FLD and TIS of PM patients, while no mutant... | PMC10114319 |
The mutational profiling of cfDNA of driver gene in peritoneal and tumor tissues | tumor | TUMOR | We investigated the mutational frequencies of driver genes in both TIS and FLD of PM and non-PM. The top driver mutations in FLD-PM were suppressor gene The mutational frequencies and sites of driver genes in peritoneal cfDNA and tumor tissues of both PM and non-PM. In scatter plots of driver gene concordance in tissue... | PMC10114319 |
Altered signaling pathways and key genes in PM | tumor | MUTANT, TUMOR | To clarify the underlying mechanism of PM, we conducted KEGG enrichment analysis to identify genetic mutational variations in the tumor tissues of PM and non-PM. The top signaling of mutant genes were comparable in PM and non-PM, including | PMC10114319 |
The cutoff value and diagnostic power of positive FLD cfDNA | tumor, CRC | TUMOR, RECURRENCE | To assess the clinical utility of peritoneal cfDNA in diagnosis of PM, we identified the cutoff value of FLD MaxAF at best positivity threshold of 6.29% performed by ROC analyses with area under curve (AUC) of 0.951 in the training cohort (Fig. The accuracy of peritoneal cfDNA for detection of PM and prediction of recu... | PMC10114319 |
Peritoneal cfDNA and RFS | tumor, CRC | TUMOR, TUMOR RECURRENCE, POSITIVE | To clarify the prognostic value of peritoneal cfDNA in CRC patients without PM, 23 CRC patients in the non-PM were followed-up from the date of surgery until tumor recurrence or until Aug 31, 2022 for patients without tumor relapse. The FLD cfDNA was sampled at the aspiration of peritoneal fluid during the surgery. The... | PMC10114319 |
Discussion | tumor, non-tumor, cancers [Primary tumor, CRC | TUMOR, TUMOR RECURRENCE, MUTANT, PERITONEAL DISSEMINATION, SOMATIC MUTATIONS | In this prospective study, we reported that peritoneal cfDNA in lavage fluid by ultradeep NGS was a reliable tool for detection of PM in CRC with high sensitivity and specificity. High concordance of genetic mutational profiling and driver genes were observed between peritoneal cfDNA and tumor tissue of CRC patients. T... | PMC10114319 |
Methods | PMC10114319 | |||
Patients and designs | tumor, CRS, CRC | PRIMARY TUMOR, TUMOR, PATHOLOGY, BLIND | This was a prospectively, open-label, blind, and diagnostic study. Patients with CRC were recruited from the Sixth Affiliated Hospital of Sun Yat-sen University, China. All enrolled patients underwent surgical resection of primary tumor or conduct CRS procedure. CRC and PM were diagnosed by pathology. Peritoneal lavage... | PMC10114319 |
Inclusion and exclusion criteria | tumor, CRC | TUMOR, METASTASIS, SECONDARY, ONCOLOGY, PATHOLOGY | Key inclusion criteria were listed as follows: consecutive patients with diagnosis of CRC and peritoneal metastasis by pathology, preoperative imaging for tumor staging, and Eastern Cooperative Oncology Group (ECOG) performance status of 0–2. Exclusion criteria included insufficient tumor tissue or peritoneal lavages, ... | PMC10114319 |
Sample collection, DNA isolation, and library preparation | MSI, tumor, Tumor, cancer, primary cancer | TUMOR, TUMOR, CANCER, MICROSATELLITE INSTABILITY, CAVITY, PRIMARY TUMOR, PRIMARY CANCER | Peritoneal lavage was collected after entering abdominal cavity during primary cancer surgery by laparotomy or laparoscopy in all enrolled patients. 300 ml of normal saline was infused into abdominal cavity through a small opening in the abdominal wall or laparoscopic trocar. About 100 ml of peritoneal lavage was aspir... | PMC10114319 |
Ultradeep NGS sequencing | tumor, UMI | TUMOR | The DNA samples from tumor tissue were ligated with regular adapters. Target capture of tissue DNA was performed using a commercial panel which consists of 520 genes (OncoScreen Plus), spanning 1.64 Mb of the human genome. The detailed genes were listed in Additional file The cfDNA from peritoneal lavage were ligated w... | PMC10114319 |
Analysis of NGS sequencing data | Sequencing data were mapped to the reference of human genome (hg19) using Burrows-Wheeler Aligner version 0.7.10 [ | PMC10114319 | ||
Gene enrichment analysis | Gene enrichment analysis was performed using the GSEA software program [ | PMC10114319 | ||
Definitions | tumor | TUMOR | The cutoff value of positivity threshold in peritoneal cfDNA was calculated according to MaxAF by ROC analyses, which allowed the best discrimination between PM and non-PM [CNVs were analyzed based on the depth of coverage data of capture intervals. Coverage data were corrected against sequencing bias from GC content a... | PMC10114319 |
Statistical analysis | All analysis was conducted using the SPSS software (v.15.0; SPSS Inc. Chicago, IL) in this study. Student's | PMC10114319 | ||
Author contributions | ZXY, WLC, TH, HW, and JC concepted and designed the study.ZXY, DL, WLC, QYQ and JC analyzed and interpreted the data. WLC, DL, TH and JC prepared cfDNA and tissue samples. ZXY, WLC, TH, XZL, CHL, XXL, HW and JC conducted ultradeep NGS assays and bioinformatic analysis. ZXY, WG, AF, TH, HW, XZL, CHL and JC drafted and r... | PMC10114319 | ||
Funding | DIGESTIVE DISEASE | This study was supported by the National Natural Science Foundation of China grant (No. 82103038, 82103084) and Guangzhou Municipal Science and Technology Program (202201011360), and National Key Research and Development Program of China (2021YFC2101700) and National Key Clinical Discipline, The Sixth Affiliated Hospit... | PMC10114319 | |
Availability of data and materials | The data can be available if requested according to the journal instructions. | PMC10114319 | ||
Declarations | PMC10114319 | |||
Ethics approval and consent to participate | This study was approved by local ethical IRB of the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. | PMC10114319 | ||
Competing interests | The authors declare no competing interest. | PMC10114319 | ||
References | PMC10114319 |
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