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Acknowledgements
STUART
We gratefully acknowledge the patients and their families for taking part of this study. The authors also acknowledge all the COMBAT-CF clinicians, nurses, study coordinators and support personnel for their invaluable contribution to this study. J.A.C.-M. thanks the director of the Curtin Health and Innovation Research...
PMC10262387
Authors’ contributions
Conceptualization, F.O.G., S.M.S., J.A.C.-M. and F.J.R.; Methodology, M.S. and J.A.C.-M.; Formal Analysis, J.A.C.-M. and S.P.A.-R.; Investigation, J.A.C.-M. and M.S.; Resources, S.M.S.; P.D.S. and R.S.W.; Writing-Original Draft Preparation, J.A.C.-M. and F.O.G.; Writing – Review & Editing, J.A.C.-M., M.S., S.P.A.-R., F...
PMC10262387
Funding
MRCG-2018–16
CYSTIC FIBROSIS, THORACIC, BROWN
S.M.S. received funding from the US Cystic Fibrosis Foundation to develop the COMBAT-CF trial (STICK10K0, STICK19K0). This work was funded in part by grants awarded by the Health Research Board (HRB-ILP-POR-2019–004), the Irish Thoracic Society (MRCG-2018–16 and MRCG-2014–6), the US Cystic Fibrosis Foundation (OGARA171...
PMC10262387
Availability of data and materials
All the sequencing data generated in this study have been deposited in the Sequencing Read Archive (BIOPROJECT PRJNA819196).
PMC10262387
Declarations
PMC10262387
Ethics approval and consent to participate
This ancillary study was part of the exploratory outcomes of the COMBAT CF study protocol. Site-specific hospital Human Research Ethics Committee approved the COMBAT-CF trial and informed consent was obtained from parents/guardians.
PMC10262387
Consent for publication
Not applicable.
PMC10262387
Competing interests
The authors declare no competing interests
PMC10262387
References
PMC10262387
Objective
rectosigmoid junction cancer, AJCC, Cancer
CANCER, RECTOSIGMOID JUNCTION CANCER
The definition of rectosigmoid junction (RSJ) is still in debate. The treatment and prognosis of patients with rectosigmoid junction cancer (RSJC) and positive lymph nodes (PLN-RSJCs) are mostly based on the American Joint Committee on Cancer (AJCC) staging system. Our study aims to assist clinicians in creating a more...
PMC10193748
Methods
Based on the Surveillance, Epidemiology, and End Results (SEER) database, we extracted 3384 patients with PLN-RSJCs and randomly divided them into development (
PMC10193748
Results
tumor, AJCC
TUMOR
Age, marital, chemotherapy, AJCC stage, T and N stage of TNM system, tumor size, and regional lymph nodes were selected as independent risk factors and included in the nomogram model. The C-index of this nomogram in the development (0.751;0.737–0.765) and validation cohorts (0.750;0.764–0.736) were more significant tha...
PMC10193748
Conclusions
We established an accurate nomogram model for PLN-RSJCs, intended to support clinicians in the treatment and follow-up of patients.
PMC10193748
Keywords
PMC10193748
Introduction
death, colorectal cancer, cancer, lung cancer, Colorectal cancer, Cancer
COLORECTAL CANCER, CANCER, LUNG CANCER, COLORECTAL CANCER, CANCER
According to the 2022 Cancer Statistics Report, about 609,360 people died from cancer in the United States. Colorectal cancer is the second leading cause of death, after lung cancer [The nomogram model is a digital simulation tool widely used in clinical research and its results can be recognized by the public [The Sur...
PMC10193748
Methods
PMC10193748
Patient selection and data processing
Data from PLN-RSJCs patients were selected from the SEER database using the SEER*Stat software version 8.4.0 (Data screening flow chart
PMC10193748
Variables defined
tumor, T stage
TUMOR
The variables in the selected cohort included: age, sex, race, marital, AJCC stage (7th), T stage, N stage, summary stage, radiotherapy, chemotherapy, tumor size, examined and positive regional nodes. To facilitate the analysis, several continuous variables (age, tumor size, examined regional nodes, and positive region...
PMC10193748
Statistical analysis
REGRESSION
For the analysis of continuous and categorical variables in both development and validation cohort, the T and Chi-square tests were used. Univariate COX regression was used to extract the potential significant predictors in the development cohort. Predictors with To assess the performance of the model, we used concorda...
PMC10193748
Results
PMC10193748
Characterization of eligible patients
The PLN-RSJCs patients (Demographics and clinical characteristics of eligible patients
PMC10193748
Nomogram construction
Based on the previous results of multivariate analysis in the development cohort, we integrated these independent prognostic factors to establish a nomogram model for OS prediction in PLN-RSJCs (Fig. Nomogram for OS prediction in PLN-RSJCsNomogram scoring system
PMC10193748
Validation of the nomogram
In the development cohort, the calculated C-index of the generated nomogram for patient OS was 0.751 (0.737–0.765), which was more significant than that of the 7th AJCC stage 0.681 (0.665–0.697). Additionally, the performances of the nomogram were assessed by ROC curves, with AUC values of 0.845, 0.808 and 0.800 for 1-...
PMC10193748
Performance of the nomogram in patient of low risk group and high risk group
The development cohort was stratified in two subgroups, according to the obtained score: low risk group: score < 94 points; high risk group: score ≥ 94 points. Kaplan–Meier survival curves showed a significant difference in OS between the two groups (Kaplan–Meier overall survival curves of PLN-RSJCs with different risk...
PMC10193748
Acknowledgements
No applicable.
PMC10193748
Special statement
All methods were carried out in accordance with relevant guidelines and regulations.
PMC10193748
Authors’ contributions
Wu Yanlong and Wang Yibing designed the research study. Wu Yanlong and Wu Yunxiao took responsibility for statistical analyses. Wu Yanlong wrote the manuscript. Wang Yibing evaluated and revised the manuscript. The author(s) read and approved the final manuscript.
PMC10193748
Funding
This work was supported by the following funds: Jiang xi Youth Science Fundation (20161BAB215250); Youth Fundation of Second Affiliated Hospital of Nanchang University (2016YNQN12029); Jiangxi Provincial Health Fund for Traditional Chinese Medicine (2019A027); Jiangxi Health and Family Planning Commission Fundation (20...
PMC10193748
Availability of data and materials
If anyone wants to request the data from this study,please contact the author named Wu Yan long(wuyanlong578@126.com).
PMC10193748
Declarations
PMC10193748
Ethics approval and consent to participate
All methods were carried out in accordance with relevant guidelines and regulations. Ethical approval for study was obtained from second Affiliated Hospital of Nanchang University Medical Research Ethics Committee. Informed consent was obtained from all the participants.We declare that none of the work contained in thi...
PMC10193748
Consent for publication
No applicable.
PMC10193748
Competing interests
The authors declare no competing interests.
PMC10193748
References
PMC10193748
Abstract
CC and NCH are joint senior authors.MAVIDOS Trial Group: Nigel K. Arden, Andrew Carr, Elaine M. Dennison, Richard Eastell, M Zulf Mughal.
PMC10470377
Background
PPH
PRETERM BIRTH, POST-PARTUM HAEMORRHAGE
Observational studies relating maternal 25-hydroxyvitamin D status to timing and mode of delivery have reported inconsistent results. We assessed the effect of antenatal cholecalciferol supplementation on the incidence of preterm birth, delivery mode and post-partum haemorrhage (PPH).
PMC10470377
Methods
PPH, blood loss
BLOOD LOSS
MAVIDOS was a randomized, double-blind, placebo-controlled trial of 1000 IU/day cholecalciferol from 14 weeks’ gestation until delivery. Gestational age, mode of delivery [categorized as spontaneous vaginal delivery (SVD), instrumental (including forceps and vacuum extraction) or Caesarean section] and PPH (>500 ml est...
PMC10470377
Results
PRETERM BIRTH
A total of 965 women participated in the study until delivery. Gestation at birth and incidence of preterm birth (cholecalciferol 5.7%, placebo 4.5%,
PMC10470377
Conclusions
PRETERM BIRTH
Antenatal cholecalciferol supplementation did not alter timing of birth or prevalence of preterm birth but demonstrated a possible effect on the likelihood of SVD.
PMC10470377
Background
rickets, seizures
PRETERM BIRTH, RICKETS, POST-PARTUM HAEMORRHAGE, CARDIOMYOPATHY, VITAMIN D DEFICIENCY, VITAMIN D DEFICIENCY
Vitamin D deficiency in pregnancy is common. In a study of predominately White women in the south of the UK, 31% had a serum 25(OH)D < 50 nmol/l (typically considered ‘insufficient’The primary function of vitamin D is in calcium and phosphate homeostasis and severe maternal vitamin D deficiency can result in neonatal h...
PMC10470377
Methods
PMC10470377
The maternal vitamin D osteoporosis study
hyperparathyroidism, ’, foetal anomalies, Osteoporosis, hypercalciuria, renal stones
HYPERPARATHYROIDISM, METABOLIC BONE DISEASE, OSTEOPOROSIS, EARLY PREGNANCY
The Maternal Vitamin D Osteoporosis Study (MAVIDOS) was a multicentre, double-blind, randomized, placebo-controlled trial of vitamin D supplementation in pregnancy. The primary outcome was neonatal bone mass. A detailed description of the study methodsWomen attending one of three United Kingdom (UK) hospitals [Universi...
PMC10470377
Intervention
Participants were randomized to either cholecalciferol 1000 IU/day or matched placebo [Merck KGaA, (Darmstadt, Germany)/Sharp Clinical Services (Crickhowell, UK; previously DHP-Bilcare)], commenced before 17 weeks’ gestation. Packs of medication were randomly assigned in a 1:1 ratio by a computer-generated sequence in ...
PMC10470377
Outcomes
PMC10470377
Maternal assessments during pregnancy
Prior to commencing the study medication, and again at 34 weeks’ gestation, the women attended the research centre for a detailed assessment lifestyle and health (smoking, medical history, current medication use) and use of vitamin D supplementation using interviewer-led questionnaires. Height and weight were measured ...
PMC10470377
Assessment of 25(OH)D
Non-fasted venous blood
Non-fasted venous blood samples were obtained on the day that the study medication was dispensed and at 34 weeks’ gestation. Serum was stored at −80°C. 25(OH)D concentration was assessed by chemiluminescence immunoassay (Liaison automated platform, Diasorin, Minnesota, USA). All samples were analysed in a single batch ...
PMC10470377
Delivery and infant details
blood loss, PPH
PRETERM BIRTH, BLOOD LOSS
Gestational age at birth and mode of delivery were collected by a research nurse/midwife from participants’ medical records. Preterm birth was defined as delivery before 37 weeks’ completed gestation. Mode of delivery was categorized as spontaneous vaginal delivery (SVD), instrumental vaginal delivery (i.e. forceps and...
PMC10470377
Statistical analysis
The analysis performed here was post hoc exploratory analysis that was not stated in the original trial protocol.Consort diagram.
PMC10470377
Ethics approval
The study was approved by the Southampton and South-West Hampshire Research Ethics Committee. MAVIDOS was registered prospectively (ISRCTN:82927713; EUDRACT:2007-001716-23); full approval from UK MHRA was granted, and written, informed consent was obtained from all participants.
PMC10470377
Results
A total of 1449 women consented to baseline 25(OH)D screening to determine the eligibility to participate in the full trial; 59 and 89 women were excluded due to 25(OH)D < 25 nmol/l and >100 nmol/l, respectively. A further 167 women withdrew prior to randomization. A total of 1134 women were initially randomized, and 9...
PMC10470377
Gestational age and birth weight at delivery
The proportion of male infants born in each group was similar (placebo 51.7%, cholecalciferol 53.9%,
PMC10470377
Mode of delivery
Mode of delivery differed between the two groups (Mode of delivery in women randomized to placebo or 1000 IU/day cholecalciferol during pregnancy (
PMC10470377
Post-partum haemorrhage
PPH
POST-PARTUM HAEMORRHAGE
PPH occurred in 32.1% of women randomized to cholecalciferol and 38.1% of women randomized to placebo (RR 0.84, 95%CI 0.71,1.00). Findings were similar for major PPH, with wider confidence limits for this less frequent outcome (RR 0.77, 95%CI 0.52,1.15). Overall, PPH was more common in women requiring a Caesarean secti...
PMC10470377
Discussion
PMC10470377
Main findings of this study
PPH
PRETERM BIRTH, EARLY PREGNANCY
In this randomized, placebo-controlled trial, 1000 IU/day cholecalciferol during pregnancy in women with an early pregnancy 25(OH)D of 25–100 nmol/l did not alter the gestational age at delivery or the incidence of preterm birth. However, our findings suggest that antenatal vitamin D supplementation might be effective ...
PMC10470377
What is already known on this topic
PRETERM BIRTH
A systematic review of intervention studies of vitamin D supplementation has not shown that supplementation reduces the risk of preterm birth.
PMC10470377
What this study adds
obesity, nerve injury, jaundice, psychological distress, PPH, bone mass, trauma
OBESITY, INTRACRANIAL HAEMORRHAGE, VITAMIN D DEFICIENCY, INFANT MORBIDITY
Supplementation with 1000 IU/day cholecalciferol did result in a difference in mode of delivery. The proportion of women having a SVD in those randomized to cholecalciferol was higher than the placebo group with fewer instrumental deliveries but no difference in Caesarean section. As instrumental delivery is associated...
PMC10470377
Limitations of this study
PREGNANCY COMPLICATIONS, VITAMIN D DEFICIENCY, RECRUITMENT, EARLY PREGNANCY
A key limitation was the exclusion of women with 25(OH)D < 25 nmol/l in early pregnancy due to ethical and governance issues. Further intervention studies are, therefore, required in women with vitamin D deficiency who might particularly benefit from supplementation. Over 95% of the MAVIDOS participants were of White e...
PMC10470377
Conclusions
PRETERM BIRTH
In conclusion, in women with a baseline 25(OH)D 25-100 nmol/l, 1000 IU/day cholecalciferol during pregnancy did not reduce the incidence of preterm birth but was associated with a modest increase in the proportion who achieved a SVD and reduction in instrumental deliveries. Further trials are required to confirm this f...
PMC10470377
Acknowledgements
C.C. and N.C.H. are joint senior authors. We are extremely grateful to Merck GmbH for the kind provision of the Vigantoletten supplement. Merck GmbH had no role in the trial execution, data collection, analysis or manuscript preparation.
PMC10470377
Data availability
Data is not publicly available. Requests for data access will be considered on application to Prof N Harvey.
PMC10470377
Funding
Arthritis
ARTHRITIS
This work was supported by grants from the Arthritis Research UK (17702), Medical Research Council (MC_PC_21003; MC_PC_21001), Bupa Foundation, National Institute for Health Research (NIHR) Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust and NIH...
PMC10470377
Disclosure summary
Arthritis
ARTHRITIS
RJM, SK, IS, RF, SVG, SD, SC, AP, SMR, AJK and MF have nothing to disclose. CC reports personal fees from ABBH, Amgen, Eli Lilly, GSK, Medtronic, Merck, Novartis, Pfizer, Roche, Servier and Takeda, outside the submitted work. NCH reports personal fees, consultancy, lecture fees and honoraria from Alliance for Better Bo...
PMC10470377
References
PMC10470377
Background
Chronic infectious wounds
Chronic infectious wounds seriously affect patients' quality of life.
PMC10782296
Aim
chronic infectious wounds
To assess the effect of whole course seamless nursing mode on patients with chronic infectious wounds.
PMC10782296
Methodology
pain
One hundred patients treated between January 2019 and December 2020 were randomly divided into control and observation groups (n=50) that were given routine nursing and whole course seamless nursing, respectively. Their pain score, comfort score, wound healing time, wound healing effect, psychological state scores, sle...
PMC10782296
Results
grade-A, pain
Observation group had lower pain score and higher comfort score than those of control group after nursing (P<0.05). Compared with control group, observation group had shorter wound healing time and higher grade-A wound healing rate (P<0.05). The SDS and SAS scores of observation group were lower than those of control g...
PMC10782296
Conclusion
chronic infectious wounds, pain
For patients with chronic infectious wounds, whole course seamless nursing effectively relieves wound pain, facilitates wound healing, improves comfort, psychological state and sleep status, and makes them more satisfied.
PMC10782296
Introduction
Chronic infectious wounds
DELAYED WOUND HEALING, INFECTION INDUCED
Chronic infectious wounds refer to the wound infection induced by the invasion of pathogenic microorganisms after injury, leading to delayed wound healing or even unhealed wounds and seriously affecting patients' quality of life
PMC10782296
Materials and methods
PMC10782296
Subjects
chronic infectious wounds, chronic infectious wounds regarding the purpose
RECRUITMENT
Recruitment was carried out by educating patients with chronic infectious wounds regarding the purpose and significance of this study, and then they voluntarily participated in the study. We conducted a prospective case-control study and included 100 patients with chronic infectious wounds treated between January 2019 ...
PMC10782296
Interventions
hypertension, hypoglycemic, diabetes
HYPERTENSION, DISEASES, COMPLICATION, DIABETES
The control group was subjected to routine nursing. Specifically, the wound was cleaned and disinfected and the dressing was changed regularly according to the doctor's advice to ensure that the dressing was clean and dry until the wound healed.Patients in observation group were given whole course seamless nursing. A w...
PMC10782296
Statistical analysis
All data were statistically analysed by SPSS 22.0 software. The categorical variables were represented as percentage and analysed using the chi-square test. The continuous numerical variables were represented as mean ± standard deviation (-x ± s). The independent t test was used for comparison between groups at the sam...
PMC10782296
Results
PMC10782296
Socio-demographic characteristics of the study population
The control group included 27 males and 23 females aged 20-65 years old, (43.25±12.65) years on average. There were 22 cases of ASA grade II and 28 cases of grade III. The observation group consisted of 29 males and 21 females aged 20-64 years old, (42.91±12.73) years on average. There were 23 cases of ASA grade II and...
PMC10782296
Pain and comfort scores
Pain, pain
Compared with before nursing, pain score was significantly lower in the two groups after nursing, especially in observation group (P<0.05). After nursing, both groups, especially control group, had significantly increased comfort scores (P<0.05) (Pain and comfort scores (x̅ ± s, point)P<0.05 P<0.05 Paired t testindepen...
PMC10782296
Wound healing results
grade-A
Compared with control group, observation group had shorter wound healing time, and higher grade-A wound healing rate (P<0.05) (Wound healing resultsP<0.05 Independent t testchi-square test
PMC10782296
Psychological state scores
Compared with before nursing, SDS and SAS scores significantly decreased in the two groups after nursing, being more obvious in observation group (P<0.05) (Psychological state scores (χ̅ ± s, point)P<0.05 P<0.05 Paired t testindependent t test
PMC10782296
Sleep indices
After nursing, both groups, particularly observation group, had significantly improved actual sleep time, sleep latency and PSQI score compared with those before nursing (P<0.05) (Sleep indices (x̅ ± s)P<0.05 P<0.05 Paired t testindependent t test
PMC10782296
Quality-of-life scores
After nursing, the two groups had significantly higher quality-of-life scores than those before nursing, especially in observation group (P<0.05) (Quality-of-life scores (x̅ ± s, point)P<0.05 P<0.05 Paired t testindependent t test
PMC10782296
Nursing satisfaction rates
The overall nursing satisfaction rate of observation group exceeded that of control group (98.00% vs. 84.00%, P<0.05) (Nursing satisfaction rates (n (%))P<0.05 Chi-square test.
PMC10782296
Discussion
chronic infectious wounds, pain, Chronic infectious wounds
Chronic infectious wounds refer to wounds (due to various factors) infected with microorganisms during the healing stage, which are common in surgeryIt is clinically recommended to perform active therapies for chronic infectious wounds, such as dressing change treatment and vacuum sealing drainageIn conclusion, the app...
PMC10782296
Acknowledgments
None.
PMC10782296
Conflict of interest
The authors have no conflicts of interest to report.
PMC10782296
ABSTRACT
PMC9970203
Background
RET, hypertrophy
HYPERTROPHY
Resistance exercise training (RET) is a common and well-established method to induce hypertrophy and improvement in strength. Interestingly, fish oil supplementation (FOS) may augment RET-induced adaptations. However, few studies have been conducted on young, healthy adults.
PMC9970203
Methods
RET
A randomized, placebo-controlled design was used to determine the effect of FOS, a concentrated source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), compared to placebo (PL) on RET-induced adaptations following a 10-week RET program (3 days·week
PMC9970203
Results
Twenty-one of 28 male and female participants (FOS,
PMC9970203
Conclusions
RET
When combined with RET, FOS improves absolute and relative 1RM upper-body and relative 1RM lower-body strength to a greater extent than that observed in the PL group of young, recreationally trained adults.
PMC9970203
KEYWORDS
PMC9970203
Background
RET, hypertrophy, FM
HYPERTROPHY
The preservation and promotion of skeletal muscle mass and strength is critical for physical performance and healthy physiology throughout the lifespan [The incorporation of EPA and DHA into skeletal muscle phospholipid has been shown to enhance both nutrient and mechanically sensitive anabolic signaling proteins known...
PMC9970203
Methods
PMC9970203
Study design
RET
A randomized, single-blind, parallel-group design was used to examine the effects of FOS compared to placebo (PL) on body composition and strength during a 10-week RET program in young adults. Regarding blinding, one of the outcome assessors was aware of the allocations; however, all outcomes were conducted with other ...
PMC9970203
Subjects
RET, chronic diseases, type-2 diabetes mellitus
HEART DISEASE, CHRONIC DISEASES, DISORDERS
Twenty-eight young male (n = 12) and female (n = 16) adults were recruited from the local central Texas area and university population for this study. All participants met the following criteria: 1) between 18 and 40 years, 2) free from neuromuscular/musculoskeletal disorders and known chronic diseases (heart disease, ...
PMC9970203
Supplementation protocol
Groups were supplemented with FO (4.5 g∙d
PMC9970203
Resistance training procedures
As illustrated in Initial training loads were selected based on 70% of the subject’s baseline 1RMs. The load was adjusted for all exercises based on the subject’s ability to reach momentary concentric failure between 8-12 repetitions. The load was decreased at the next training session if the subject completed less tha...
PMC9970203
Dietary records
RET
Subjects were required to submit three-day food logs (two weekdays, one weekend day) before and after the RET intervention using the MyFitnessPal mobile or desktop application (MyFitnessPal; San Francisco, CA, USA). Additionally, LC n-3 PUFA intake was assessed using a food frequency questionnaire (FFQ) that has been v...
PMC9970203
Fatty acid dried blood spot
FAPS
REGRESSION
Fatty acid dried blood spot (DBS) was obtained to track supplementation compliance and ensure adequate LC n-3 PUFA membrane incorporation. A drop of blood was collected from each participant via finger stick on filter paper pre-treated with a preservation solution (Fatty Acid Preservative Solution, FAPS™) and allowed t...
PMC9970203
Statistical analyses
All statistical analyses were performed using IBM SPSS version 28 (Armonk, NY, USA). Data were tested for normality and homogeneity using the Shapiro-Wilks and Levene’s tests, respectively. Baseline characteristics were analyzed using an independent samples
PMC9970203