id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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40,392 | Generation of iPSC-derived dopaminergic neurons | 4 | dx.doi.org/10.17504/protocols.io.bjpgkmjw | https://www.protocols.io/view/generation-of-ipsc-derived-dopaminergic-neurons-bjpgkmjw | Julie Jerber, James Haldane, Juliette Steer, Daniel Pearce, Minal Patel | TITLE: Generation of iPSC-derived dopaminergic neurons
AUTHORS: Julie Jerber, James Haldane, Juliette Steer, Daniel Pearce, Minal Patel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes a method for the production of dopaminergic neurons from human iPSCs, using a 52-day l... | ["[Seeding iPSCs for neural induction (Day -1)]\nAspirate the media from the well(s) of the iPSC culture and add of DPBS (-/-) to wash.\n2 mL", "[Seeding iPSCs for neural induction (Day -1)]\nAspirate the DPBS (-/-) and add of warm Accutase to each well.\n1 mL", "[Seeding iPSCs for neural induction (Day -1)]\nIncubat... |
108,829 | Crystallisation of SARS-CoV-2 N Protein | 1 | dx.doi.org/10.17504/protocols.io.8epv5r8ydg1b/v1 | https://www.protocols.io/view/crystallisation-of-sars-cov-2-n-protein-dnh55b86 | Peter Marples, Lizbé Koekemoer, Daren Fearon | TITLE: Crystallisation of SARS-CoV-2 N Protein
AUTHORS: Peter Marples, Lizbé Koekemoer, Daren Fearon
[DESCRIPTION]
The crystallization protocol and buffer conditions used to obtain reproducible SARS C0V-2 Nucelocapsid crystals suitable for XChem fragment screening.
[STEPS]
SECTION: Crystallisation experiment
3. Prot... | ["[Crystallisation experiment] Protein and buffer requirements:\n57.6 µL20 mg/mL \n2.88 mL \n5.76 µL seeds, dilution 1:100", "[Crystallisation experiment] Dispense 30 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.\nDispense 200 nL20 mg/mL to each lens using the SPT mosquito.\nD... |
35,748 | Ligation Protocol for NEB PCR Cloning Kit (E1202) | 1 | dx.doi.org/10.17504/protocols.io.be6cjhaw | https://www.protocols.io/view/ligation-protocol-for-neb-pcr-cloning-kit-e1202-be6cjhaw | New England Biolabs | TITLE: Ligation Protocol for NEB PCR Cloning Kit (E1202)
AUTHORS: New England Biolabs
[DESCRIPTION]
Ligation protocol for the PCR Cloning Kit (E1202).
[BEFORE_START]
For purified PCR amplicon products, the amount of insert to be added can be calculated by relative length or molar calculations. See the Guidelines f... | ["Assemble ligation reactions using the chart below as a guide. Mix the first 4 components before adding 5 µL consisting of 4 µL and 1 µL, for a total of 10 µL per ligation reaction. \n \n\n \n LIGATION REACTIONPOSITIVE CONTROLLinearized pMiniT Vector (25 ng/μl)1 μl (25 ng)1 μl (25 ng)Insert*1–4 μl*–Amplicon Cloning C... |
80,772 | SARS-CoV-2 Main Protease (Mpro) Fluorescence Dose Response | 1 | null | https://www.protocols.io/view/sars-cov-2-main-protease-mpro-fluorescence-dose-re-cs5cwg2w | Haim Barr, Noa Lahav | TITLE: SARS-CoV-2 Main Protease (Mpro) Fluorescence Dose Response
AUTHORS: Haim Barr, Noa Lahav
[DESCRIPTION]
This is a functional, biochemical assay used to identify treatments for viral infectious disease that target SARS-COV-2 Main Protease (MPro).
Utilizing a direct enzyme activity measurement method, the experi... | ["[Prepare 384 Well Plate] PRIME Multi-Drop Combi Tube Dispensing Cassette with Assay Buffer by selecting the PRIME button on the Combi Dispenser until the tubes are filled completely.", "[Prepare 384 Well Plate] DISPENSE 10 µL to Columns 1 and 23 of assay plate\nNote: These will represent the inhibitor control column... |
89,963 | L-4 LEECH TESTING | 4 | dx.doi.org/10.17504/protocols.io.n2bvj3z6plk5/v1 | https://www.protocols.io/view/l-4-leech-testing-c34jyqun | REDI-NET Consortium | TITLE: L-4 LEECH TESTING
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol details standard operating procedure for leech testing.
[BEFORE_START]
BEFORE START
Check the DNA and RNA concentrations in each sample of total nucleic acid (TNA) extraction.
If the concentrations are detectable, choose the sequencin... | ["[gDNA PREPARATION] When the RNA concentration of the sample is lower than the detectable range of the Qubit High Sensitivity Assay (<0.01 ng/µl), the sample is subjected to gDNA sequencing. The cDNA synthesis can be skipped.", "[gDNA PREPARATION] When the DNA concentration >10 ng/µl, calculate the required volume of ... |
30,665 | Anti-Neu5Gc Antibody Kit Protocol - Western Blot | null | dx.doi.org/10.17504/protocols.io.97hh9j6 | null | Sam Li | TITLE: Anti-Neu5Gc Antibody Kit Protocol - Western Blot
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The Anti-Neu5Gc Antibody Kit contains the essential monospecific polyclonal chicken IgY antibody, along with a negative control primary antibody to detect the presence of Neu5Gc on... | ["Prepare two identical SDS-PAGE gels.", "Load and run samples in loading buffer.", "Recommended loading of gel:Lane 1: Molecular Weight MarkerLanes 2, 8, 10: BlankLane 3-6: SamplesLane 7: Positive ControlLane 9: Negative Control", "Confirm the presence of proteins on gel by Coomassie Staining", "Blot proteins to membr... |
104,683 | In vivo microdialysis for striatal DA release | 0 | dx.doi.org/10.17504/protocols.io.n92ld8q1xv5b/v1 | https://www.protocols.io/view/in-vivo-microdialysis-for-striatal-da-release-digj4bun | Ariadna Laguna, Miquel Vila | TITLE: In vivo microdialysis for striatal DA release
AUTHORS: Ariadna Laguna, Miquel Vila
[DESCRIPTION]
To assess local effects of nomifensine (DAT and NET inhibitor) on striatal DA
release in microdialysis experiments
[STEPS]
SECTION: Preparation of reagents
1. Nomifensine (DAT and NET inhibitor) is dissolved in art... | ["[Preparation of reagents] Nomifensine (DAT and NET inhibitor) is dissolved in artificial cerebrospinal fluid (aCSF in mM: NaCl, 125; KCl, 2.5; CaCl2, 1.26 and MgCl2 1.18)", "[Mouse surgery] One concentric dialysis probe (Cuprophan membrane; 6000 Da molecular weight cut-off; 1.5 mm-long) is implanted in the striatum (... |
59,918 | CODEX® Multiplexed Imaging | Modality Overview | 1 | dx.doi.org/10.17504/protocols.io.36wgq7dryvk5/v1 | https://www.protocols.io/view/codex-multiplexed-imaging-modality-overview-b6rnrd5e | Diane Saunders, Conrad Reihsmann, Alexander Hopkirk, Marcela Brissova, Alvin C. Powers | TITLE: CODEX® Multiplexed Imaging | Modality Overview
AUTHORS: Diane Saunders, Conrad Reihsmann, Alexander Hopkirk, Marcela Brissova, Alvin C. Powers
[DESCRIPTION]
This is an overview of all protocols currently in use by the Vanderbilt Diabetes Research Center Islet & Pancreas Analysis (IPA) Core and Powers/Brissova ... | ["Procure and/or prepare samples fixed with 4% paraformaldehyde (PFA).\n\nHuBMAP | VU TMC Eye/Pancreas | Human Pancreas Processing for Multiple Applications (HuBMAP)\nMouse Pancreas Dissection and Fixation for Cryosectioning (IPA Core)", "Prepare coverslips with pancreas sections.\n\n \n\nⓘ Samples mounted on coverslip... |
52,687 | Criteria to evaluate neurological lower urinary tract dysfunction in children with Congenital Zika Syndrome: Revised version after the fourth year of use | 1 | dx.doi.org/10.17504/protocols.io.bxpppmmn | https://www.protocols.io/view/criteria-to-evaluate-neurological-lower-urinary-tr-bxpppmmn | Juliana Marin Fontes, Glaura Cruz, Rafael Pauletti Gonçalves, Tereza Cristina Melo, Átila Rondon, Adriano Calado, Lucia Maria Costa Monteiro | TITLE: Criteria to evaluate neurological lower urinary tract dysfunction in children with Congenital Zika Syndrome: Revised version after the fourth year of use
AUTHORS: Juliana Marin Fontes, Glaura Cruz, Rafael Pauletti Gonçalves, Tereza Cristina Melo, Átila Rondon, Adriano Calado, Lucia Maria Costa Monteiro
[... | ["[Initial assessment of urological screening:]\nInitial assessment of urological screening:The initial assessment comprises a detailed clinical history, a comprehensive physical examination, lab tests, the urinary ultrasonography and the urodynamic study.prenatal and \tmaternal history related to Zika (rash during pre... |
30,787 | Cell Surface Flow Cytometry Staining of Whole Blood | null | dx.doi.org/10.17504/protocols.io.babbiain | null | Sam Li | TITLE: Cell Surface Flow Cytometry Staining of Whole Blood
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. Add predetermined optimum concentrations of desired fluorochrome conjugated, biotinylated, or purified primary antibodies to 100µl of anti-coagulated whole blood.
?. Incubate at room t... | ["Add predetermined optimum concentrations of desired fluorochrome conjugated, biotinylated, or purified primary antibodies to 100µl of anti-coagulated whole blood.", "Incubate at room temperature for 15-20 minutes in the dark.", "Dilute 10X Red Blood Cell (RBC) Lysis Buffer (BioLegend Cat. No. 420301) to 1X working co... |
null | null | null | dx.doi.org/10.17504/protocols.io.kggcttw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Se realiza la extracción de la fracción rica en leucocitos a partir de sangre total usando la sedimentación de los eritrocitos en una solución con carboximetil celulosa e iodioxanol al 12%. La extracción de los PMNs a partir de dicha fracción en un gradiente discontinuo de de... | [] |
102,968 | qPCR standard for library quantification | 3 | dx.doi.org/10.17504/protocols.io.bp2l6xwd5lqe/v2 | https://www.protocols.io/view/qpcr-standard-for-library-quantification-dgsy3wfw | Ayinuer Aximu Petri, Matthias Meyer | TITLE: qPCR standard for library quantification
AUTHORS: Ayinuer Aximu Petri, Matthias Meyer
[DESCRIPTION]
Protocol for the preparation of a standard for DNA library quantification by quantitative real-time PCR (Meyer et al. 2008; Gansauge et al. 2020).
References
Meyer, M., Briggs, A. W., Maricic, T., Höber, B., H... | [] |
104,329 | Liquid-liquid extraction of 3,3'-dichlorobiphenyl (PCB11) and its hydroxylated metabolites from animal blood/serum | 1 | dx.doi.org/10.17504/protocols.io.36wgq3w8xlk5/v2 | https://www.protocols.io/view/liquid-liquid-extraction-of-3-3-39-dichlorobipheny-dh5h3836 | Xueshu Li, Maxwell W Shumaker, Hansjoachim Lehmler | TITLE: Liquid-liquid extraction of 3,3'-dichlorobiphenyl (PCB11) and its hydroxylated metabolites from animal blood/serum
AUTHORS: Xueshu Li, Maxwell W Shumaker, Hansjoachim Lehmler
[DESCRIPTION]
This protocol describes the extraction of 3,3'-dichlorobiphenyl (PCB11) and its monohydoxylated metabolites from mouse ... | ["[Sample preparaiton] Place whole blood (0.3-0.5 g) or serum (~ 0.2 g) in tube-A. Record weight on the working sheet.", "[Addition of Analytical Standards] Spike all tube-A samples (MB, TB, tissue, OPR, and Reference standard) with SS_PCB (10 ng d-PCB65 and 10 ng PCB14). \n d-PCB65, 100 µL x 100 ng/mL = 10 ng each\n P... |
63,392 | Condor CBD Gummies Reviews [2022]:Is It Worth the Money?(Scam or Legit) - Don't Buy Till You Read!– 【Official Website ✔️✔️✔️】 | 1 | dx.doi.org/10.17504/protocols.io.kqdg3py4ql25/v1 | https://www.protocols.io/view/condor-cbd-gummies-reviews-2022-is-it-worth-the-mo-b958r89w | Condor CBD Gummies | TITLE: Condor CBD Gummies Reviews [2022]:Is It Worth the Money?(Scam or Legit) - Don't Buy Till You Read!– 【Official Website ✔️✔️✔️】
AUTHORS: Condor CBD Gummies
[DESCRIPTION]
Condor CBD Gummies
[STEPS]
1. Condor CBD Gummies
➢Product Name —Condor CBD Gummies Reviews
➢Main Benefits—Improve Metabolism & Help i... | ["Condor CBD Gummies\n\n \n\n\n➢Product Name —Condor CBD Gummies Reviews\n➢Main Benefits—Improve Metabolism & Help in Pain Relief\n➢Composition —NaturalOrganic Compound\n➢Side-Effects—NA\n➢Rating :—⭐⭐⭐⭐⭐\n➢Availability —Online\n➢Price (for Sale) Buy Now Here —Click Here\nCondor CBD Gummies - You plan to recuperate just... |
45,324 | Protein Concentration Determination using Qubit | 4 | null | https://www.protocols.io/view/protein-concentration-determination-using-qubit-bqhkmt4w | Steven Burgess | TITLE: Protein Concentration Determination using Qubit
AUTHORS: Steven Burgess
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Procedure for quantification of protein concentration. This version is adapted for use with samples that have been extracted in protein extraction buffer as part of pr... | ["[Create Working Solution for Analysis]\nCreate a working solution of Qubit assay buffer by diluting the reagent 1:200 in the provided buffer.\nThe final volume in each tube must be 200 µL. Each standard tube requires 190 µL of Qubit® working solution, and each sample tube requires anywhere from 180–199 µL. Prepare su... |
null | null | null | dx.doi.org/10.17504/protocols.io.ejqbcmw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol outlines how to generate tables to analyze CRISPR interactions. Based on methods from the following publication:<br /><br />Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and Dynam... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.k2ccyaw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is a DNA extraction protocol adapted from Byron Crump's lab, which was developed from both Zhou et al. 1996 and Crump et al. 2003. It is intended to be used with 47 mm 0.22 um PES filters or a 0.22 uM Sterivex filter, both preserved with RNALater after sample collection.... | [] |
61,626 | Donor eligibility Criteria for Human Liver for HuBMAP TTD-Columbia/PSU | 1 | null | https://www.protocols.io/view/donor-eligibility-criteria-for-human-liver-for-hub-b8e2rtge | Presha Rajbhandari, Hua Tian, Helen Remotti, Brent Stockwell, bs | TITLE: Donor eligibility Criteria for Human Liver for HuBMAP TTD-Columbia/PSU
AUTHORS: Presha Rajbhandari, Hua Tian, Helen Remotti, Brent Stockwell, bs
[DESCRIPTION]
This documents outline the criteria for human liver tissue donor eligibility for the Hubmap BioMolecular Atlas Program (HuBMAP), Transformative... | ["[Inclusion criteria for Donor:] -Age range >18\n-BMI <29\n-All race, ethnicity and gender (equal male and female) are accepted\n-Negative for HIV, Hepatitis B or C virus, CMV or EBV, COVID-19, sepsis\n-Negative for liver diseases including fatty liver disease, hepatocellular carcinoma, and diabetes", "[Sample:] -Visu... |
98,540 | Traces referential on raw material from Foz do Chapecó (Brazil) | 0 | dx.doi.org/10.17504/protocols.io.5qpvokynzl4o/v1 | https://www.protocols.io/view/traces-referential-on-raw-material-from-foz-do-cha-dcgk2tuw | Lou Igier, Lilla Tamara Jakab, Vikram SHRESTHA | TITLE: Traces referential on raw material from Foz do Chapecó (Brazil)
AUTHORS: Lou Igier, Lilla Tamara Jakab, Vikram SHRESTHA
[DESCRIPTION]
Quite a few lithic use-wear referentials on raw materials from Brazil exist. To further develop use-wear analysis in the country and especially in the region of Foz do Chapecó, n... | ["[Documentation of the experimental flakes before use] Macroscopic and microscopic observation and documentation of the state of the flakes before use with naked eye, with a camera\n \n and a Dino-lite microscope.", "[Washing of the used experimental flakes] Wash for 20 minutes in an ultrasound tank\n \n in a neutral ... |
19,401 | Wireless electrochemical measurement of kanamycin in whole blood | 1 | dx.doi.org/10.17504/protocols.io.j8nlk5dydl5r/v1 | https://www.protocols.io/view/wireless-electrochemical-measurement-of-kanamycin-w7hfhj6 | Jun-Chau Chien, Alexandra E. Rangel, Hyongsok T. Soh | TITLE: Wireless electrochemical measurement of kanamycin in whole blood
AUTHORS: Jun-Chau Chien, Alexandra E. Rangel, Hyongsok T. Soh
[DESCRIPTION]
This work evaluates the life-time of the structural-switching aptamer sensors in the whole blood using in vitro blood circulation setup as the first step toward implantabl... | ["[Aptamer Immobilization] Use heat-shrink and bare gold wire (250 um) to define the sensing area at the wire tip.", "[Aptamer Immobilization] Solvent clean with sonication in acetone, methanol, and DI-water.", "[Aptamer Immobilization] Perform electrochemical cleaning using 3-wire electrochemical system \na. 3 CV cycl... |
53,022 | Hybridization Chain Reaction combined with Immunohistochemistry for Whole-Mount Embryos | 1 | dx.doi.org/10.17504/protocols.io.bxz6pp9e | https://www.protocols.io/view/hybridization-chain-reaction-combined-with-immuno-bxz6pp9e | Ali M Elagoz, Ruth Styfhals, Sofia Maccuro, Luca Masin, Lieve Moons, Eve Seuntjens | TITLE: Hybridization Chain Reaction combined with Immunohistochemistry for Whole-Mount Embryos
AUTHORS: Ali M Elagoz, Ruth Styfhals, Sofia Maccuro, Luca Masin, Lieve Moons, Eve Seuntjens
[DESCRIPTION]
Here, we are providing an optimized, step-by-step clearing protocol that retains the signal generated by HCR v3.0 i... | ["[Embryo Fixation and Preparation] DAY 0\n\nFix tissue overnight in 4 % paraformaldehyde (PFA) in phosphate-buffered saline (PBS-DEPC) at 4°C.", "[Embryo Fixation and Preparation] DAY 1\n\nWash with PBS-DEPC.", "[Embryo Fixation and Preparation] Dechorionate the octopus embryos in PBST.", "[Dehydration] Dehydrate embr... |
null | null | null | dx.doi.org/10.17504/protocols.io.rdhd236 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<div>
<div>
<div>
<div>
<p>This assay was designed and developed by Alyssa Pyke and Bruce Harrower at this laboratory aided by design software in use at the time.</p>
<p>The assay specifically targets the haemagglutinin (HA) region of influenza A(H1)pdm09 virus strains and... | [] |
67,423 | Official website:-https://wintersupplement.com/instahard-reviews/ | 1 | dx.doi.org/10.17504/protocols.io.eq2lynkdwvx9/v1 | https://www.protocols.io/view/official-website-https-wintersupplement-com-instah-cd37s8rn | Exipure Reviews | TITLE: Official website:-https://wintersupplement.com/instahard-reviews/
AUTHORS: Exipure Reviews
[DESCRIPTION]
InstaHard Reviews
[STEPS]
1. Official website:-https://wintersupplement.com/instahard-reviews/
InstaHard
InstaHard Reviews
Unique Offer on InstaHard™:
This moment there's an extraordinary limited offer tha... | ["Official website:-https://wintersupplement.com/instahard-reviews/\nInstaHard\nInstaHard Reviews\nUnique Offer on InstaHard™:\nThis moment there's an extraordinary limited offer that saves you 53%-73% off on InstaHard™ relying upon the size of your request. This exceptional proposition incorporates 5 unconditional gif... |
54,773 | Preparation of cells for transmission electron microscopy ultrastructural analysis | 4 | dx.doi.org/10.17504/protocols.io.j8nlk47ewg5r/v1 | https://www.protocols.io/view/preparation-of-cells-for-transmission-electron-mic-bzqvp5w6 | Jillian C Danne, Simon A Crawford, Rachel Templin, Joan Clark, Viola Oorschot, Georg Ramm | TITLE: Preparation of cells for transmission electron microscopy ultrastructural analysis
AUTHORS: Jillian C Danne, Simon A Crawford, Rachel Templin, Joan Clark, Viola Oorschot, Georg Ramm
[DESCRIPTION]
A transmission electron microscope (TEM) enables the magnification and visualisation of cell and tissue ultrastruc... | ["[Fixation] All fixation and processing steps must be performed in a fume hood wearing the appropriate personal protective equipment (PPE). The Material Safety Data Sheet (MSDS) for each chemical must be read before commencing.\n\nAdherent cells grown in a well-plate or petri dish must be submerged in solution at all ... |
66,624 | Select Keto (Newest Report!): Best Health Select Keto, Improves the Energy Levels of the Body and Provides Elevated Stamina. | 3 | dx.doi.org/10.17504/protocols.io.bp2l616zkvqe/v1 | https://www.protocols.io/view/select-keto-newest-report-best-health-select-keto-cda8s2hw | victorwilsons | TITLE: Select Keto (Newest Report!): Best Health Select Keto, Improves the Energy Levels of the Body and Provides Elevated Stamina.
AUTHORS: victorwilsons
[DESCRIPTION]
select keto
[STEPS] | [] |
100,677 | Serapure Preparation and Testing | 1 | null | https://www.protocols.io/view/serapure-preparation-and-testing-dejd3ci6 | rute.carvalho Carvalho | TITLE: Serapure Preparation and Testing
AUTHORS: rute.carvalho Carvalho
[DESCRIPTION]
This protocol is used to prepare low-cost SPRI beads used for Illumina Library preparations. As part of the Hakai Institute Ocean Observing Program, from 0 m to near bottom (260 m), biomolecular samples have been collected weeklyto g... | ["[QUALITY CONTROL] The following image compares the results of “purifying” a mix of 2 µL Fermentas Ultra Low\nRange Ladder + 18 µL dH20 using several different amounts of AMPure or Serapure solution to\nDNA solution. AMPure is on the left, “Serapure” is on the right. After preparing 20 µL of ladder\n+ water mix, we co... |
75,716 | ALS and the Gut-Brain Axis: A protocol for a systematic review and meta-analysis assessing the relationship between amyotrophic lateral sclerosis, the gut and its microbiome. | 3 | dx.doi.org/10.17504/protocols.io.5qpvorqpzv4o/v1 | https://www.protocols.io/view/als-and-the-gut-brain-axis-a-protocol-for-a-system-cm7cu9iw | Alexander T Jackson, Rollo G. Press, fergal waldron, Jenna Gregory | TITLE: ALS and the Gut-Brain Axis: A protocol for a systematic review and meta-analysis assessing the relationship between amyotrophic lateral sclerosis, the gut and its microbiome.
AUTHORS: Alexander T Jackson, Rollo G. Press, fergal waldron, Jenna Gregory
[DESCRIPTION]
Abstract
Background: ALS is a devastating neur... | [] |
41,170 | X-Prize Protocol | 3 | null | https://www.protocols.io/view/x-prize-protocol-bkfsktne | vincent.degennaro | TITLE: X-Prize Protocol
AUTHORS: vincent.degennaro
[STEPS] | [] |
45,281 | Summary of the housing and husbandry conditions of Zebrafish (Danio rerio) at University of Glasgow | 4 | dx.doi.org/10.17504/protocols.io.bqf9mtr6 | https://www.protocols.io/view/summary-of-the-housing-and-husbandry-conditions-of-bqf9mtr6 | Anita Racz, Brooke Allan, Toni Dwyer, Davide Thambithurai, Amélie Crespel, Shaun S. Killen | TITLE: Summary of the housing and husbandry conditions of Zebrafish (Danio rerio) at University of Glasgow
AUTHORS: Anita Racz, Brooke Allan, Toni Dwyer, Davide Thambithurai, Amélie Crespel, Shaun S. Killen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The environmental and housing condition... | ["[Housing conditions]\nThe detailed information on the housing and husbandry conditions can be find in the attached PDF:"] |
50,397 | Procedures for Performing the Mixed-Meal Tolerance Test (Appendix 2 of "Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus") | 1 | dx.doi.org/10.17504/protocols.io.bvf5n3q6 | https://www.protocols.io/view/procedures-for-performing-the-mixed-meal-tolerance-bvf5n3q6 | Stephen.Gitelman , Jeffrey A. Bluestone | TITLE: Procedures for Performing the Mixed-Meal Tolerance Test (Appendix 2 of "Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus")
AUTHORS: Stephen.Gitelman , Jeffrey A. Bluestone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is Appendix ... | [] |
100,988 | Detection of SARS CoV-2 in different samples with a singleplex and mutation resistant real time PCR assay | 0 | dx.doi.org/10.17504/protocols.io.5qpvokkkzl4o/v1 | https://www.protocols.io/view/detection-of-sars-cov-2-in-different-samples-with-deu43eyw | Sudhir Bhatia, Gudrun Baersch | TITLE: Detection of SARS CoV-2 in different samples with a singleplex and mutation resistant real time PCR assay
AUTHORS: Sudhir Bhatia, Gudrun Baersch
[DESCRIPTION]
Genekam has developed an unique ready to use PCR kit for the detection of SARS CoV-2 indifferent samples. This was developed on 20.01.2020 just at the be... | ["Thaw one tube each: A, B, Y, D1 and D2. After thawing, put the tubes at 4°C (as it is better). If the kit\nis not in use, store them at -20°C.", "Mark your microtubes with a sample number, +ve Control and –ve Control. Otherwise use a 96 well microplate instead of tubes.", "Add 7µl of tube A to each tube.", "Add 10µl... |
99,517 | Sugar Beet Tissue Collection for Genome Assembly and Annotation with Long Read Sequencing | 4 | dx.doi.org/10.17504/protocols.io.rm7vzbk92vx1/v2 | https://www.protocols.io/view/sugar-beet-tissue-collection-for-genome-assembly-a-dde523g6 | Olivia Todd, kevin.dorn | TITLE: Sugar Beet Tissue Collection for Genome Assembly and Annotation with Long Read Sequencing
AUTHORS: Olivia Todd, kevin.dorn
[DESCRIPTION]
This protocol outlines the various types of tissue collection needed to follow the USDA-ARS Dorn lab protocol for Beta vulgaris crop wild relative genome sequencing. The annot... | ["Follow the protocol below as written to isolate plant nuclei from 1-5g of dark treated tissue.\nhttps://15a13b02-7dac-4315-baa5-b3ced1ea969d.filesusr.com/ugd/5518db_90f751986a4a4e7bbc8e648d467507d2.pdf?index=true", "Check for quality and presence of necessary DNA size for sequencing (>15kb) using a Pippin Pulse, runn... |
92,347 | Fixed RNA - FFPE Resection Tissue (gentleMACS dissociation) | 4 | dx.doi.org/10.17504/protocols.io.eq2lyj4wrlx9/v1 | https://www.protocols.io/view/fixed-rna-ffpe-resection-tissue-gentlemacs-dissoci-c6e3zbgn | Ksenija Sabic | TITLE: Fixed RNA - FFPE Resection Tissue (gentleMACS dissociation)
AUTHORS: Ksenija Sabic
[DESCRIPTION]
This protocol utilizes the gentleMACS OctoDissociator, for pestle dissociation please refer to the original 10x protocol. This protocol assumes multiplexing exactly 4 or 16* samples with 8,000 cells per sample, if f... | ["[Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling] Buffer Preparation (~20 min min):", "[Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling] Transfer either one or two FFPE scrolls to a gentleMACS C tube keeping the scrolls intact.\nNote: Scrolls need to be ... |
61,940 | PROTAC GMP Services | 6 | dx.doi.org/10.17504/protocols.io.5qpvobq7zl4o/v1 | https://www.protocols.io/view/protac-gmp-services-b8qurvww | boc.protac | TITLE: PROTAC GMP Services
AUTHORS: boc.protac
[DESCRIPTION]
BOC Sciences’ GMP production sites were designed in strict accordance to US-FDA guidelines for GMP Manufacturing. It has passed US-FDA inspections. We provide standard QC tests before releasing GMP products. For GMP products, we guarantee >98% purity. In a... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.rx3d7qn | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
46,297 | SARS-CoV-2 Whole Genome Sequencing on Illumina | 1 | dx.doi.org/10.17504/protocols.io.brfzm3p6 | https://www.protocols.click/view/sars-cov-2-whole-genome-sequencing-on-illumina-brfzm3p6 | Guerrino Macori, Lauren Russell, Seamus Fanning | TITLE: SARS-CoV-2 Whole Genome Sequencing on Illumina
AUTHORS: Guerrino Macori, Lauren Russell, Seamus Fanning
[DESCRIPTION]
This SOP describes the procedure for generating cDNA from SARS-CoV-2 viral nucleic acid extracts and subsequently obtaining, through the amplicons tiling, the whole viral genome using V3 nCov-2... | ["[SARS-CoV-2 WvGS protocol - cDNA Preparation Reverse Transcription] cDNA/Reverse Transcription Section Date/Initials:_________________\n\n\n\nIn this section, the nucleic acid is extracted and used for the qPCR diagnostic test as starting material for sequencing.", "[SARS-CoV-2 WvGS protocol - cDNA Preparation... |
64,397 | Simpli ACV Keto Gummies Reviews: Fraud Attention, Is Simply Health safe or Scam? Where to Buy!! | 3 | dx.doi.org/10.17504/protocols.io.j8nlkkrjwl5r/v1 | https://www.protocols.io/view/simpli-acv-keto-gummies-reviews-fraud-attention-is-ca5msg46 | alexhalex | TITLE: Simpli ACV Keto Gummies Reviews: Fraud Attention, Is Simply Health safe or Scam? Where to Buy!!
AUTHORS: alexhalex
[DESCRIPTION]
Simpli ACV Keto Gummies are the high level weight reduction recipe that is upheld by natural substances to offer regular weight reduction results. The recipe is clinically supported... | [] |
82,352 | Extraction of 1-HP derivatives and HPLC preparation | 4 | dx.doi.org/10.17504/protocols.io.5jyl8j43dg2w/v1 | https://www.protocols.io/view/extraction-of-1-hp-derivatives-and-hplc-preparatio-cunqwvdw | Muhammad Zaka Asif, Man Shah, Yosef Smadi | TITLE: Extraction of 1-HP derivatives and HPLC preparation
AUTHORS: Muhammad Zaka Asif, Man Shah, Yosef Smadi
[DESCRIPTION]
This protocol describes day 6 of our workflow to grow worms in liquid culture to induce the production of natural products (in this case, 1-HP derivatives). In this protocol, we extract 1-HP and ... | ["Remove the sample from the lyophilizer. It should appear to be a powder.", "Resuspend in minimal methanol (enough to submerge the pellet)", "Vortex the tube with methanol to aid in dissolution.", "The HPLC injection sample caps out at 90 uL, so minimizing the solution volume here will minimize the total number of inj... |
94,653 | 2. PERFF-seq: HCR Flow-FISH and Polymer Disassembly Protocol | 1 | dx.doi.org/10.17504/protocols.io.8epv5x8r4g1b/v1 | https://www.protocols.io/view/2-perff-seq-hcr-flow-fish-and-polymer-disassembly-c8n5zvg6 | Tsion Abay, Robert Stickels, Meril Takizawa, Ronan Chaligne, Caleb Lareau | TITLE: 2. PERFF-seq: HCR Flow-FISH and Polymer Disassembly Protocol
AUTHORS: Tsion Abay, Robert Stickels, Meril Takizawa, Ronan Chaligne, Caleb Lareau
[DESCRIPTION]
This protocol can be used for performing HCR Flow-FISH followed by cell/nuclei enrichment via FACS and polymer disassembly on fixed and permeabilized cel... | ["[Detection Stage] After performing cell/nuclei preparation, pre-warm hybridization buffer and thermomixer with a heated lid to 37 °C.", "[Detection Stage] Add 400uL of pre-warmed hybridization buffer per 1 million (1M) cells/nuclei.\nFor cells, incubate in a thermomixer at 37 °C for 30 minutes (300rpm shaking) or a r... |
22,414 | Testing selective agents for the Icthyosporeans Abeoforma whisleri, Pirum gemata and Spaheroforma artica | null | dx.doi.org/10.17504/protocols.io.z5nf85e | null | Elena Casacuberta, Cristina Aresté | TITLE: Testing selective agents for the Icthyosporeans Abeoforma whisleri, Pirum gemata and Spaheroforma artica
AUTHORS: Elena Casacuberta, Cristina Aresté
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Minimal protocol for testing selective agents for </span><span style = "font-style:italic;... | ["Seed cells on 24 well/plate. Density depends on the organism but should allow to screen for growth and death easilySeed the same density to test four or five different dilutions of each drugSeed enough wells to have 3X replicates for each conditionSeeded cells for Abeoforma whisleri, Pirum gemata and Spaheroforma art... |
50,786 | Laboratory Protocols for Ancient and Modern Dental Calculus DNA Processing (Fellows Yates et al. 2021) | 2 | dx.doi.org/10.17504/protocols.io.bvuan6se | https://www.protocols.io/view/laboratory-protocols-for-ancient-and-modern-dental-bvuan6se | James Fellows Yates, Irina Velsko, Franziska Aron, Courtney Hofman, Christina Warinner | TITLE: Laboratory Protocols for Ancient and Modern Dental Calculus DNA Processing (Fellows Yates et al. 2021)
AUTHORS: James Fellows Yates, Irina Velsko, Franziska Aron, Courtney Hofman, Christina Warinner
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Collection of protocols used for Fellows... | [] |
69,165 | Intravagal AAV injection in mice | 4 | dx.doi.org/10.17504/protocols.io.3byl4je2olo5/v1 | https://www.protocols.io/view/intravagal-aav-injection-in-mice-cfsmtnc6 | Ayse Ulusoy, Michael Helwig, Angela Rollar, Michael Klinkenberg, Sinead O'Sullivan, Shirley Lee, Rita Pinto-Costa, Donato Di Monte | TITLE: Intravagal AAV injection in mice
AUTHORS: Ayse Ulusoy, Michael Helwig, Angela Rollar, Michael Klinkenberg, Sinead O'Sullivan, Shirley Lee, Rita Pinto-Costa, Donato Di Monte
[DESCRIPTION]
The protocol details AAV vector injections into the mouse vagus nerve and describes pre and post-operative mouse handling. ... | ["Anesthetize the mouse with isoflurane and use pain killers (e.g. Temgesic) following the ethical committee procedures", "Clean the surgery area with skin disinfectant, make a small incision at the midline of the neck, and\nisolate the left vagus nerve. Place a holder underneath to keep it isolated and to form a base ... |
58,601 | Extraction of DNA from Frozen Islet Repository Samples | 4 | dx.doi.org/10.17504/protocols.io.j8nlkkw11l5r/v1 | https://www.protocols.io/view/extraction-of-dna-from-frozen-islet-repository-sam-b5ghq3t6 | IIDP-HIPP | TITLE: Extraction of DNA from Frozen Islet Repository Samples
AUTHORS: IIDP-HIPP
[DESCRIPTION]
This Standard Operating Procedure (SOP) provides Human Islet Phenotyping Program (HIPP) procedures for DNA extraction from frozen islet pellets using the Promega Wizard® Genomic DNA Purfication Kit.
This SOP defines the a... | ["[Procedures] Assemble all items described in Materials.", "[Procedures] Make Nuclei Lysis Buffer: Mix 500 µL Nuclei Lysis Solution with 120 µL 0.5M EDTA PH 8.0. Chill on ice.", "[Procedures] Add 600 µL chilled Nuclei Lysis Buffer and 20 µL proteinase K to the islet pellet. Vortex vigorously for 10 seconds. Incubate a... |
37,519 | Building and Using the Illuminated Orbital Shaker | null | dx.doi.org/10.17504/protocols.io.bgvpjw5n | https://www.protocols.io/view/building-and-using-the-illuminated-orbital-shaker-bgvpjw5n | Jakub Nedbal | TITLE: Building and Using the Illuminated Orbital Shaker
AUTHORS: Jakub Nedbal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The building of a bottom-illuminated orbital shaker designed for the cultivation of microalgae suspensions is described in this protocol collection. The instrument agitates ... | [] |
53,729 | Zeiss LSM880 with Fast Airyscan Guide | 1 | null | https://www.protocols.io/view/zeiss-lsm880-with-fast-airyscan-guide-byp9pvr6 | Condon ND | TITLE: Zeiss LSM880 with Fast Airyscan Guide
AUTHORS: Condon ND
[DESCRIPTION]
<div class = "text-blocks"><div style = "text-align :; float : ;"><img style = "" src = "https://s3.amazonaws.com/protocols-files/files/egrbbm7s7.jpg" /></div><br/></div>
[STEPS]
?. Start-up Procedure
?. [System Start-up]
-Turn the Main sw... | ["Start-up Procedure", "[System Start-up]\n-Turn the Main switch ON (1) You should not have to touch the key since this should never be turned off-Turn the Systems/PC switch ON (2)-Turn the PC ON and wait for it to boot up completely-Log in to the PC using your AD login details-Turn Components ON (3)-Double-click the Z... |
63,891 | Keto Now Reviews: Do Keto Now Diet Pills Work or Scam? | 3 | dx.doi.org/10.17504/protocols.io.4r3l2obd4v1y/v1 | https://www.protocols.io/view/keto-now-reviews-do-keto-now-diet-pills-work-or-sc-camtsc6n | aldorijimbo | TITLE: Keto Now Reviews: Do Keto Now Diet Pills Work or Scam?
AUTHORS: aldorijimbo
[DESCRIPTION]
Have you settled on the choice to deal with your weight in a characteristic way? Is it true that you are continuously thinking about ketosis, the Ketone diet, and Keto Now containers? Is it true or not that you are exhau... | [] |
74,896 | Phosphate Buffered Saline (PBS) | 4 | null | https://www.protocols.io/view/phosphate-buffered-saline-pbs-cmdqu25w | Andreas Sagen | TITLE: Phosphate Buffered Saline (PBS)
AUTHORS: Andreas Sagen
[DESCRIPTION]
Phosphate-buffered saline (PBS) is a buffer solution (pH ~7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and optionally, potassium chloride and potassium dih... | ["[100 mL 10x PBS w/o Ca2++, Mg2++] All compounds are measured using a high precision analytical scale from powdered compounds. Each compound is measured to within 1% of the target weight. All compounds are mixed in a Duran bottle", "[100 mL 10x PBS w/o Ca2++, Mg2++] Fill the bottle with 50 mL double-distilled water", ... |
null | null | null | dx.doi.org/10.17504/protocols.io.s7hehj6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
49,747 | Plant-associated microbiome sampling protocol for field work | 1 | dx.doi.org/10.17504/protocols.io.buttnwnn | https://www.protocols.io/view/plant-associated-microbiome-sampling-protocol-for-buttnwnn | Cassie Ettinger, Marina LaForgia | TITLE: Plant-associated microbiome sampling protocol for field work
AUTHORS: Cassie Ettinger, Marina LaForgia
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details sampling of plant-associated microbiomes from plant communities in field plots.</div></div>
[STEPS]
?. [Microbiome Samp... | ["[Microbiome Sampling Protocol]\nTake a photo of the subplot plot or subplot to be sampled\na. Make sure the photo is GPS tagged!. b. From the photo, we can later get time of sampling and approximate GPS location.", "[Microbiome Sampling Protocol]\nTake any environmental measurements at this time (e.g. temperature, ... |
95,914 | Doering search for Xu SR 2023 malingering | 1 | dx.doi.org/10.17504/protocols.io.e6nvwdwozlmk/v1 | https://www.protocols.io/view/doering-search-for-xu-sr-2023-malingering-c9wiz7ce | Michelle Doering | TITLE: Doering search for Xu SR 2023 malingering
AUTHORS: Michelle Doering
[DESCRIPTION]
This protocol details the Doering search for Xu SR 2023 malingering.
[GUIDELINES]
Methods section text:
The published literature was searched using strategies created by a medical librarian for the demographics of patients test... | ["[Ovid Medline] exp malingering/ or Malinger*.mp. or (feign* adj4 (psychopatholog* or symptom*)).mp. or feigned.mp. or conscious-simulation.mp. or pathomimia.mp.\t4136", "[Ovid Medline] exp Emergency Medical Services/ or exp hospital emergency service/ or exp forensic psychiatry/ or emergicenter*.ti,ab. or (emergency ... |
91,112 | F-3 FECES STORAGE | 4 | dx.doi.org/10.17504/protocols.io.yxmvm3qn9l3p/v1 | https://www.protocols.io/view/f-3-feces-storage-c48gyztw | REDI-NET Consortium | TITLE: F-3 FECES STORAGE
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol describes feces storage.
[GUIDELINES]
OBJECTIVE
To outline steps for properly storing feces samples and nucleic acid samples purified from these samples.
SUMMARY/SCOPE
The overarching aim of the REDI-NET is to develop a collaborative... | ["[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Precool 96-well microfuge tube racks on ice.", "[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Using preprinted adhesive labels or permanent markers, label 1.5 ml microfuge tubes with unique sample ID.", "[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Once the rack is full or all feces sa... |
68,400 | Preparation of mollusc larval shells for individual geochemical analysis | 1 | dx.doi.org/10.17504/protocols.io.bp2l61jwkvqe/v1 | https://www.protocols.io/view/preparation-of-mollusc-larval-shells-for-individua-ce2qtgdw | Vincent Mouchi, Thomas Broquet, Thierry Comtet | TITLE: Preparation of mollusc larval shells for individual geochemical analysis
AUTHORS: Vincent Mouchi, Thomas Broquet, Thierry Comtet
[DESCRIPTION]
This protocol describes the digestion process of mollusc larvae and is modified from the procedure found in Becker et al. (2005). Here, we indicate the entire process to... | ["[Preparation of the digestion solution (for 20 mL final volume of approximately 15% H2O2)] In a glass beaker\nAdd 10 mL Suprapur® hydrogen peroxide (H2O2) 30%", "[Preparation of the digestion solution (for 20 mL final volume of approximately 15% H2O2)] Place the beaker with hydrogen peroxide on a magnetic stirrer an... |
80,830 | Prospective Hospital-based patient survey | 1 | dx.doi.org/10.17504/protocols.io.261ge3m5yl47/v1 | https://www.protocols.io/view/prospective-hospital-based-patient-survey-cs66whhe | Kajiru Gad Kilonzo, Stefanie J. Krauth, Jo Halliday, Clive Kelly, Stefan Siebert, Gloria Temu, Christopher Bunn, Nateiya M Yongolo, Sally Wyke, Emma McIntosh, Blandina Mmbaga | TITLE: Prospective Hospital-based patient survey
AUTHORS: Kajiru Gad Kilonzo, Stefanie J. Krauth, Jo Halliday, Clive Kelly, Stefan Siebert, Gloria Temu, Christopher Bunn, Nateiya M Yongolo, Sally Wyke, Emma McIntosh, Blandina Mmbaga
[DESCRIPTION]
This protocol details prospective hospital-based patient survey.
[STEPS... | ["[Prospective Hospital-based patient survey] The aim of the prospective patient survey is to estimate the burden of musculoskeletal disorders including arthritis and other associated conditions at KCMC. In addition, the prospective study will help identify patients for selection for the qualitative component of the st... |
63,699 | Lipids in microalgae: Quantitation by acid-dichromate method in microtiter plate | 1 | dx.doi.org/10.17504/protocols.io.e6nvw9dpzgmk/v2 | https://www.protocols.io/view/lipids-in-microalgae-quantitation-by-acid-dichroma-caftsbnn | Yingyu Hu, Zoe V Finkel | TITLE: Lipids in microalgae: Quantitation by acid-dichromate method in microtiter plate
AUTHORS: Yingyu Hu, Zoe V Finkel
[DESCRIPTION]
This is a protocol for quantitating total lipids in microalgae.
The acid-dichromate method is widely used to perform colorimetric analysis of extracted lipids. Here we present a pr... | ["[Preparation of Standard] Prepare glyceryl tripalmitate (GTP) primary standard solution (around 1 mg/ml)", "[Preparation of Standard] Place frozen GTP in vacuum desiccator with lose cap until it is warmed to Room temperature before making primary standard solution", "[Preparation of Standard] Weigh around 1 mg GTP,... |
57,204 | Monitoring fly feeding behavior and timing by beetle luciferase reporters | 1 | dx.doi.org/10.17504/protocols.io.b34uqqww | https://www.protocols.io/view/monitoring-fly-feeding-behavior-and-timing-by-beet-b34uqqww | Misha Koksharov | TITLE: Monitoring fly feeding behavior and timing by beetle luciferase reporters
AUTHORS: Misha Koksharov
[DESCRIPTION]
Monitoring Drosophila feeding behavior usually requires laborious procedures and custom-made equipment. This protocol describes how feeding behavior of individual flies carrying a beetle (e.g. fir... | ["[Preparation of a 96-well plate with a luciferin-containing food] Prepare a hot fly food mix and keep it unsolidified by placing a tube with food into a 48-50°C water bath.50 °C \n\na) It can be a regular complex cooked fly food (such as BDSC Cornmeal Food).\nb) It can be various synthetic and semi-defined formulatio... |
39,265 | Marmoset NHP Free Floating Anti-GFP Antibody Stain After HCR FISH | 4 | null | https://www.protocols.io/view/marmoset-nhp-free-floating-anti-gfp-antibody-stain-bij9kcr6 | Guoping Feng Lab | TITLE: Marmoset NHP Free Floating Anti-GFP Antibody Stain After HCR FISH
AUTHORS: Guoping Feng Lab
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A protocol for staining NHP tissue for anti-GFP antibody after staining for markers of intrest with HCR FISH.</div></div>
[STEPS]
?. [Primary Antibody]
... | ["[Primary Antibody]\nPermeabilized marmoset brain sections (, fixed previously in PFA) in Triton X-100 in PBS for .", "[Primary Antibody]\nBlock sections with NS (natural serum depending on Secondary Antibody) and PBT [ Triton X-100, 1X PBS] for .", "[Primary Antibody]\nIncubate primary antibody (1:500 whole mount... |
null | null | null | dx.doi.org/10.17504/protocols.io.izhcf36 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
95,776 | Extraction, derivatisation and GC-MS/MS of d2-glucose and d5-glycerol from human plasma | 1 | null | https://www.protocols.io/view/extraction-derivatisation-and-gc-ms-ms-of-d2-gluco-c9r8z59w | Joanna P Simpson, Scott G Denham, Natalie ZM Homer | TITLE: Extraction, derivatisation and GC-MS/MS of d2-glucose and d5-glycerol from human plasma
AUTHORS: Joanna P Simpson, Scott G Denham, Natalie ZM Homer
[DESCRIPTION]
Tracer glucose and tracer glycerol is used to assess glucose kinetics and turnover.
Here we have improved upon existing gas chromatography mass spect... | ["[Glucose and Glycerol and tracer acetate derivative analysis by GC-MS/MS] Set up an acquisition batch in Xcalibur software using the electronic Microsoft Excel file of the calibration standards and sample list. Set to inject 2 µL per sample and use a method of chromatographic separation as described in step 10 and ma... |
104,196 | Opentrons Pipeline: DNA Extraction with the Mag-Bind Blood & Tissue DNA HDQ 96 Kit Tissue Protocol | 0 | dx.doi.org/10.17504/protocols.io.bp2l62rqrgqe/v1 | https://www.protocols.io/view/opentrons-pipeline-dna-extraction-with-the-mag-bin-dhzc372w | Thalía Silvestre | TITLE: Opentrons Pipeline: DNA Extraction with the Mag-Bind Blood & Tissue DNA HDQ 96 Kit Tissue Protocol
AUTHORS: Thalía Silvestre
[DESCRIPTION]
This protocol is an automated pipeline to extract a full 96-well plate of DNA from tissue lysates.
This protocol was developed and optimized for the following:
Platform:... | ["[List of materials to start to use OT-2] It is necessary to have this equipment to use this pipeline.\nOpentrons Equipment List:", "[List of materials to start to use OT-2] Tips & Labware:", "[Samples Lysis] Preparate 96 samples lysis following the Mag-Bind‱ Blood & Tissue DNA HDQ 96 Kit Tissue Protocol:", "[Star OT-... |
76,167 | Isolating Plasmodium sexual and asexual parasites from malaria infected individuals for single cell RNA sequencing | 4 | dx.doi.org/10.17504/protocols.io.4r3l27e7pg1y/v1 | https://www.protocols.click/view/isolating-plasmodium-sexual-and-asexual-parasites-cnmfvc3n | Sunil Kumar Dogga, Antoine Dara, Jesse Rop, Abdoulaye Djimdé, Arthur Talman, Mara Lawniczak | TITLE: Isolating Plasmodium sexual and asexual parasites from malaria infected individuals for single cell RNA sequencing
AUTHORS: Sunil Kumar Dogga, Antoine Dara, Jesse Rop, Abdoulaye Djimdé, Arthur Talman, Mara Lawniczak
[DESCRIPTION]
This protocol isolates circulating sexual and asexual Plasmodium parasites from an... | ["[Blood Draw] Draw fresh blood into a CPDA vacutainer, mix by inverting 4-5 times and immediately keep on ice (Heparin might inhibit PCR/RT. EDTA affects parasites if left for a few hours).", "[Blood Draw] Following fresh blood draw or transportation as above, add 5 mL of cold SA buffer to 3 mL of subject blood sam... |
83,265 | Modified Rapid Phytolith Methods | 1 | dx.doi.org/10.17504/protocols.io.3byl4qerzvo5/v1 | https://www.protocols.click/view/modified-rapid-phytolith-methods-cvi9w4h6 | Kali R Wade | TITLE: Modified Rapid Phytolith Methods
AUTHORS: Kali R Wade
[DESCRIPTION]
Processing procedures here are modified from those outlined in Katz et al 2010. Samples can be processed in this way and, following completion, rinsed with deionized water and dried to a stable state for storage and to make extra slides for mor... | ["[Fail-Safe Practices] Wiping down all equipment, surfaces, and tools used with soap and water followed by acetone (beginning and end of every day of the project).", "[Fail-Safe Practices] Checking and adjusting the density of sodium polytungstate (SPT) to conform to the protocol being used (normally between 2.3 and 2... |
19,346 | RNA Sequencing of Porine Ganglia and brainstem | null | dx.doi.org/10.17504/protocols.io.w5sfg6e | null | Leah Reznikov | TITLE: RNA Sequencing of Porine Ganglia and brainstem
AUTHORS: Leah Reznikov
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">RNA Sequencing of Porine Ganglia and brainstem</span><span style = "font-weight:bold;font-weight:bold;">
</span><span style = "font-weight:bo... | [] |
74,001 | Scott's Tap Water Substitute | 6 | null | https://www.protocols.io/view/scott-39-s-tap-water-substitute-ckhrut56 | joshdm | TITLE: Scott's Tap Water Substitute
AUTHORS: joshdm
[DESCRIPTION]
Used as a bluing agent in H&E staining
[STEPS]
SECTION: Measure and mix salts
1. weigh 20 g of
SECTION: Measure and mix salts
2. weigh 2 g of
SECTION: Measure and mix salts
3. Mix in ddWater until completely dissolved
store at room tempera... | ["[Measure and mix salts] weigh 20 g of", "[Measure and mix salts] weigh 2 g of", "[Measure and mix salts] Mix in ddWater until completely dissolved\nstore at room temperature\npH 8.3 to pH 8.7"] |
57,352 | High School Optogenetics Workshop Protocol | 1 | dx.doi.org/10.17504/protocols.io.b39gqr3w | https://www.protocols.io/view/high-school-optogenetics-workshop-protocol-b39gqr3w | Liudi Luo, Bryce W Hina, Brennan W McFarland, Jillian C Saunders, Natalie Smolin, and Catherine R. von Reyn | TITLE: High School Optogenetics Workshop Protocol
AUTHORS: Liudi Luo, Bryce W Hina, Brennan W McFarland, Jillian C Saunders, Natalie Smolin, and Catherine R. von Reyn
[DESCRIPTION]
Although the field of neurotechnology is predicted to grow at a tremendous rate and become a part of our everyday lives, we have ... | ["[Optogenetic Activation] After positioning the fly and adjusting the camera, initiate a video recording and a 300ms red light stimulation (measured to be 0.1605 mW/mm2 at the fly’s location) by touching the record button on the phone. Click the record button again to end the video recording which now contains the fl... |
null | null | null | dx.doi.org/10.17504/protocols.io.r3dd8i6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes a method of collecting, displaying and analysing ground reaction force (GRF) vector patterns in horses during locomotion. The method may be applied to other quadrupeds.</p>
[STEPS]
?.
?.
?. | [] |
86,178 | Free-floating Mouse Brain Immunohistochemistry | 4 | null | https://www.protocols.io/view/free-floating-mouse-brain-immunohistochemistry-cyeaxtae | Jonathan Breiter | TITLE: Free-floating Mouse Brain Immunohistochemistry
AUTHORS: Jonathan Breiter
[DESCRIPTION]
This protocol enables immunohistochemical staining of murine tissue with superior penetration of the tissue by the reagents due to the free-floating approach.
[STEPS]
SECTION: Tissue Preparation
1. Remove PFA-fixed tissue fr... | ["[Tissue Preparation] Remove PFA-fixed tissue from storage solution and add to mesh bottom netwell insert inside of 12 well plate that is filled with 0.22 μm filtered 1x PBS.", "[Buffer Preparation] Per well of tissue make a minimum of 3.5 mL of blocking solution, consider this is needed for blocking, primary and seco... |
37,370 | pcp-2338 child protocol | null | null | https://www.protocols.io/view/pcp-2338-child-protocol-bgq2jvye | Maria Guliakina | TITLE: pcp-2338 child protocol
AUTHORS: Maria Guliakina
[STEPS]
?. step | ["step"] |
85,043 | Workflow for bulk RNAseq of human fallopian tube and uterine endomyometrium | 4 | dx.doi.org/10.17504/protocols.io.4r3l224p3l1y/v1 | https://www.protocols.click/view/workflow-for-bulk-rnaseq-of-human-fallopian-tube-a-cxatxien | Scott Lindsay-Hewett, Valentina Stanley, Mana Parast, Louise Laurent | TITLE: Workflow for bulk RNAseq of human fallopian tube and uterine endomyometrium
AUTHORS: Scott Lindsay-Hewett, Valentina Stanley, Mana Parast, Louise Laurent
[DESCRIPTION]
Described here is the workflow used by the Female Reproductive Tissue Mapping Center at UCSD to generate bulk RNAseq data from human fallopian ... | ["[Tissue preparation] As soon as possible after sterilization (salpingectomy or tubal ligation), prepare fallopian tube tissue according to the following protocol:\n\nHuman Pregnant Fallopian Tube Tissue Collection and Preservation Methods - UCSD Female Reproductive TMC\n\nAt the time of C-section, prepare uterine end... |
44,510 | Postoperative Rehabilitation of Total Knee Arthroplasty | 1 | dx.doi.org/10.17504/protocols.io.bpp6mmre | https://www.protocols.io/view/postoperative-rehabilitation-of-total-knee-arthrop-bpp6mmre | Luisa Mululo, Fabricio Loures, Ana Carla Gonzaga de Oliveira, Cleiton Ferreira, Guilherme Morgado Runco | TITLE: Postoperative Rehabilitation of Total Knee Arthroplasty
AUTHORS: Luisa Mululo, Fabricio Loures, Ana Carla Gonzaga de Oliveira, Cleiton Ferreira, Guilherme Morgado Runco
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol was created by the rehabilitation team of the Hospital Univers... | [] |
26,236 | Preparation of JF dye for retro-orbital injection in mice | null | dx.doi.org/10.17504/protocols.io.5u4g6yw | null | Boaz Mohar | TITLE: Preparation of JF dye for retro-orbital injection in mice
AUTHORS: Boaz Mohar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>How to prepare </span><a href="https://www.janelia.org/open-science/janelia-fluor-dyes" style = "text-decoration:underline;color:blue;cursor:pointer;"><span styl... | ["[Dye preperation]\nTake an aliqote with of dye from storage.\n-20 °C", "[Dye preperation]\nAdd DMSO and do ~10 up and down with the pipeter\n20 µl", "[Dye preperation]\nAdd 20ul Pluronic F-127 20% in DMSO ~10 up and down with the pipeter", "[Dye preperation]\nAdd 200ul 1x PBS ~10 up and down with the pipeter", "... |
null | null | null | dx.doi.org/10.17504/protocols.io.ci9uh5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Developing a Titration Kill Curve (G418, Hygromycin B and Puromycin)
[BEFORE_START]
Each mammalian cell line has a different sensitivity. Before experimentation, you should determine the optimal concentration of your antibiotic by developing the Titration Kill Curve for your ce... | [] |
90,156 | Zymo Protocol | 4 | dx.doi.org/10.17504/protocols.io.kxygx3ooog8j/v1 | https://www.protocols.io/view/zymo-protocol-c4akyscw | Emily Gailey | TITLE: Zymo Protocol
AUTHORS: Emily Gailey
[DESCRIPTION]
Zymo Automation
[STEPS]
SECTION: 1-Step PCR
1. Set up a master mix according to the table below:
SECTION: 1-Step PCR
2. For each reaction, add 14 μl of the master mix to the appropriate wells of a 96-well real-time PCR plate. A sample of the plate setup ca... | ["[1-Step PCR] Set up a master mix according to the table below:", "[1-Step PCR] For each reaction, add 14 μl of the master mix to the appropriate wells of a 96-well real-time PCR plate. A sample of the plate setup can be found on the next page and on the Plate Setup Guide.", "[1-Step PCR] Index Primer Addition:\n\na. ... |
61,802 | Quantification of SARS-CoV-2 variant mutations (HV69-70, E484K/N501Y, del156-157/R158G, del143-145, and LPPA24S) in settled solids using digital RT-PCR | 1 | dx.doi.org/10.17504/protocols.io.14egnzrrzg5d/v6 | https://www.protocols.io/view/quantification-of-sars-cov-2-variant-mutations-hv6-b8kiruue | Bridgette Hughes, Bradley J. White, Marlene K. Wolfe, Alexandria B B Boehm | TITLE: Quantification of SARS-CoV-2 variant mutations (HV69-70, E484K/N501Y, del156-157/R158G, del143-145, and LPPA24S) in settled solids using digital RT-PCR
AUTHORS: Bridgette Hughes, Bradley J. White, Marlene K. Wolfe, Alexandria B B Boehm
[DESCRIPTION]
This process instruction describes the steps for quantitativ... | ["[Preparation] Retrieve all kit components from the One-Step RT-ddPCR advanced kit for probes from the -20 °C freezer and thaw the components on ice.", "[Preparation] Retrieve ddPCR positive control aliquots (50 copies per uL gRNA) from the -80 °C freezer and thaw on ice", "[Preparation] For re-running frozen plates o... |
67,076 | Condor CBD Gummies – Majestic Ingredients | 1 | dx.doi.org/10.17504/protocols.io.5jyl897b7v2w/v1 | https://www.protocols.io/view/condor-cbd-gummies-majestic-ingredients-cdrcs52w | fluxactiverate | TITLE: Condor CBD Gummies – Majestic Ingredients
AUTHORS: fluxactiverate
[DESCRIPTION]
Condor CBD GummiesEveryone merits the best wellbeing joy, fulfillment, and by and large prosperity! It is actually the case that this isn't not difficult to accomplish with out the assistance of Condor CBD Gummies. These extraordi... | [] |
27,224 | Hydra Dissociation/Reaggregation | null | dx.doi.org/10.17504/protocols.io.6tyhepw | null | Yashodara Abeykoon, Adrienne Cho, Celina Juliano | TITLE: Hydra Dissociation/Reaggregation
AUTHORS: Yashodara Abeykoon, Adrienne Cho, Celina Juliano
[STEPS]
?. [Dissociation of Hydra]
Wash 30 Hydra several times in fresh Hydra Medium (HM) to remove debris
?. [Dissociation of Hydra]
Transfer Hydra to 1.5mL Eppendorf, remove media, and add 1mL 4°C Dissociation Medium (... | ["[Dissociation of Hydra]\nWash 30 Hydra several times in fresh Hydra Medium (HM) to remove debris", "[Dissociation of Hydra]\nTransfer Hydra to 1.5mL Eppendorf, remove media, and add 1mL 4°C Dissociation Medium (DM)", "[Dissociation of Hydra]\nIncubate Hydra at 4°C for 30-60 min (can incubate several hours without obv... |
90,186 | Immunocapture of virion from body fluids | 1 | dx.doi.org/10.17504/protocols.io.e6nvwdom7lmk/v1 | https://www.protocols.io/view/immunocapture-of-virion-from-body-fluids-c4biyske | Jeffrey A. Johnson, Sarah Sabour, Jin-fen Li, Jonathan Lipscomb | TITLE: Immunocapture of virion from body fluids
AUTHORS: Jeffrey A. Johnson, Sarah Sabour, Jin-fen Li, Jonathan Lipscomb
[DESCRIPTION]
Procedure for immunocapturing HIV virions from blood and seminal plasma, cerebral spinal fluid, and cell culture supernatant by monoclonal antibody-targeting source cell markers in vir... | ["[Concentrate Antibody] Use Amicon Ultra-0.5 Centrifugal Filter Devices.", "[Concentrate Antibody] Insert the Amicon device into the microcentrifuge tube.", "[Concentrate Antibody] Add up to 500 µL Ab (0.1 2897-0.2 2897) to the filter device and cap it.", "[Concentrate Antibody] Insert the capped Amicon Ultra device i... |
40,363 | Chicken immunization with KLH-gp120 fragment (254-274) conjugate. | 4 | dx.doi.org/10.17504/protocols.io.bjnjkmcn | https://www.protocols.io/view/chicken-immunization-with-klh-gp120-fragment-254-2-bjnjkmcn | Angel Justiz-Vaillant | TITLE: Chicken immunization with KLH-gp120 fragment (254-274) conjugate.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Chicken immunization with peptides is inefective if only just the peptides are being inoculated. However, to make the immune response effective the... | ["Two healthy layer chickens (brown Leghorn), aged approximately 6 months, are injected intramuscularly at multiple sites on the breast with the peptide-keyhole limpet hemocyanin (KLH) conjugate.", "The chickens are immunized on day 0, with 0.2 µmol/ml of the fragment 254-274 of HIV gp120-conjugated to KLH (immunogen) ... |
67,838 | pool-seq pipeline - Stanford et al. | 5 | null | https://www.protocols.io/view/pool-seq-pipeline-stanford-et-al-ceg6tbze | brenna.cm.stanford | TITLE: pool-seq pipeline - Stanford et al.
AUTHORS: brenna.cm.stanford
[DESCRIPTION]
Determining cryptic species and diversity in at-risk species is necessary for the understanding and conservation of biodiversity. The endangered Banff Springs Snail, Physella johnsoni, inhabits seven highly specialized thermal spring... | ["[Project information and background] Project information \n\nBelow is the pipeline I used to analyze my pool-seq data (Stanford 2019). For my project, I analyzed five populations of Physella johnsoni and three populations of Physella gyrina of 20 to 40 individuals per population. These were sequenced by Genome Quebe... |
47,278 | Purification protocol of Mouse (Mus Musculus) E1-like enzyme ATG7 | 4 | dx.doi.org/10.17504/protocols.io.bsennbde | https://www.protocols.io/view/purification-protocol-of-mouse-mus-musculus-e1-lik-bsennbde | Dorotea Fracchiolla | TITLE: Purification protocol of Mouse (Mus Musculus) E1-like enzyme ATG7
AUTHORS: Dorotea Fracchiolla
[DESCRIPTION]
This protocol outlines the procedures for expression and purification of Mouse (Mus Musculus) E1-like enzyme ATG7 (AuTophaGy-related protein) of the ATG8 ubiquitin-like conjugation system in autophagy.
... | ["[Infection/expression/harvest] Infect 1 L of Sf9 cells growing in Sf921 medium with Penicillin/Streptomycin at 1-1.5 mil/ml cells/volume at 99-100% viability in log phase with Virus 1 (V1) according to viral titer. \n(NOTE: protein yield from 1 lt culture is high, e.i. 32 mg/1lt culture).", "[Infection/expression/har... |
61,008 | Shipping wastewater samples to FDA-CFSAN | 1 | dx.doi.org/10.17504/protocols.io.14egn797zv5d/v3 | https://www.protocols.io/view/shipping-wastewater-samples-to-fda-cfsan-b7tqrnmw | Maria Balkey, Tina.Pfefer | TITLE: Shipping wastewater samples to FDA-CFSAN
AUTHORS: Maria Balkey, Tina.Pfefer
[DESCRIPTION]
This SOP provides guidance for shipping wastewater samples to the US FDA Center for Food Safety and Applied Nutrition (CFSAN), covering specific steps for registering samples with CFSAN and for the preparation of shipm... | ["[Submission of metadata] Fill out the BioSample custom wastewater template (extension of NCBI's Generic SARS-CoV-2: wastewater surveillance, v1.0 ) according to the NCBI submission protocol for SARS-Cov-2 protocol, once completed, send it to covidtrakr@fda.hhs.gov for registration. Successful registrations will rece... |
90,821 | In vitro phosphorylation | 1 | dx.doi.org/10.17504/protocols.io.ewov1q71ogr2/v1 | https://www.protocols.io/view/in-vitro-phosphorylation-c4xdyxi6 | Leonardo A Parra-Rivas | TITLE: In vitro phosphorylation
AUTHORS: Leonardo A Parra-Rivas
[DESCRIPTION]
In vitro phosphorylation
[STEPS]
1. 5 μg of α-syn GST fusion protein on beads were
resuspended in a buffer containing 20 mM HEPES, 1 mM ATP, 10 mM MgCl2,
2 mM DTT, pH 7.4.
2. The reaction was initiated by adding 1 ug of human
recombinan... | ["5 μg of α-syn GST fusion protein on beads were\nresuspended in a buffer containing 20 mM HEPES, 1 mM ATP, 10 mM MgCl2,\n2 mM DTT, pH 7.4.", "The reaction was initiated by adding 1 ug of human\nrecombinant PLK2 (Abcam, Cat#ab102108) and incubating at 30 °C with orbital\nshaking overnight (Eppendorf ThermoMixer F1.5)."... |
55,798 | Preparation of acute midbrain slices containing the superior colliculus and periaqueductal gray for patch-clamp recordings | 4 | dx.doi.org/10.17504/protocols.io.8epv51pz5l1b/v5 | https://www.protocols.io/view/preparation-of-acute-midbrain-slices-containing-th-b2qwqdxe | Oriol Pavon Arocas, Tiago Branco | TITLE: Preparation of acute midbrain slices containing the superior colliculus and periaqueductal gray for patch-clamp recordings
AUTHORS: Oriol Pavon Arocas, Tiago Branco
[DESCRIPTION]
This protocol is a practical guide for preparing acute coronal slices from the midbrain of young adult mice for electrophysiology exp... | ["[Preparation of stock solutions]", "[Preparation of stock solutions] [PATH A - Decapitation under terminal isoflurane anaesthesia]\n \n\n\nSlicing ACSF (sucrose)\n\nFor each experiment you will need ~500 mL of solution: ~150 mL for the first recovery chamber and ~350 mL to make the slush used during the slicing steps... |
94,871 | DAB Staining for GFP on Free-floating Fixed NHP Brain Tissue | 4 | dx.doi.org/10.17504/protocols.io.eq2lyjy4mlx9/v1 | https://www.protocols.io/view/dab-staining-for-gfp-on-free-floating-fixed-nhp-br-c8vxzw7n | Aydin Alikaya, William Stauffer | TITLE: DAB Staining for GFP on Free-floating Fixed NHP Brain Tissue
AUTHORS: Aydin Alikaya, William Stauffer
[DESCRIPTION]
This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex to detect Green ... | ["[Part I (Day 1)] Bring tissue to Room temperature in Phosphate Buffered Saline (PBS, pH 7.2-7.4) on an orbital shaker for 30 minutes. 30 min", "[Part I (Day 1)] Prepare Peroxide Solution (0.3 % H2O2) in dH2O.\nFor 10 mL 0.3% H2O2 use:\n100 µL 30% H2O2 \n9900 µL dH2O", "[Part I (Day 1)] Prepare Normal Goat Serum Block... |
null | null | null | dx.doi.org/10.17504/protocols.io.deh3b5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For use in "<a href="http://protocols.io/view/FASP-Kit-Protocol-ORNL-Developed-for-Bacteriophage-ddn25d" target="_blank">FASP Kit Protocol-OrNL Developed for Bacteriophage</a>"
[STEPS]
?.
?.
?.
?. | [] |
56,592 | Collection of Protocols for transcardial perfusion, immunohistochemistry/immunofluorescence and dual in situ hybridization | 2 | dx.doi.org/10.17504/protocols.io.kqdg3p5xel25/v1 | https://www.protocols.io/view/collection-of-protocols-for-transcardial-perfusion-b3hqqj5w | Michael X. Henderson | TITLE: Collection of Protocols for transcardial perfusion, immunohistochemistry/immunofluorescence and dual in situ hybridization
AUTHORS: Michael X. Henderson
[DESCRIPTION]
This is a collection of protocols about transcardial perfusion, immunohistochemistry/immunofluorescence and dual in situ hybridization.
[STEPS] | [] |
50,687 | Comparative study in safety and efficacy of maintenance therapies for patients with anti-neutrophil cytoplasmic antibody small vessel vasculitis: A network meta-analysis | 1 | dx.doi.org/10.17504/protocols.io.bvq7n5zn | https://www.protocols.io/view/comparative-study-in-safety-and-efficacy-of-mainte-bvq7n5zn | Ioannis Bellos, Sophia Lionaki | TITLE: Comparative study in safety and efficacy of maintenance therapies for patients with anti-neutrophil cytoplasmic antibody small vessel vasculitis: A network meta-analysis
AUTHORS: Ioannis Bellos, Sophia Lionaki
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Anti-neutrophil cytoplasmic antibo... | ["Background Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis represents a potentially life-threatening disease characterized by vascular inflammation, endothelial and tissue injury. In most patients, ANCAs against leukocyte proteinase 3 (PR3) or myeloperoxidase (MPO) are detected. Necrotizing vasculit... |
35,828 | Isolation of SARS-Cov2 RNA from Humans Without High Demand Reagents | null | dx.doi.org/10.17504/protocols.io.be8ujhww | https://www.protocols.io/view/isolation-of-sars-cov2-rna-from-humans-without-hig-be8ujhww | Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton | TITLE: Isolation of SARS-Cov2 RNA from Humans Without High Demand Reagents
AUTHORS: Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Viral RNA isolation kits used in PCR based tests for the novel coronavirus (SARS-Cov2) ar... | ["Transfer the swab into a DNase/RNase free 1.5 mL microcentrifuge tube. •NOTE: The swabs we use can be pulled off their stick for easier processing.", "Add 500 µL of TRIzol to the microcentrifuge tube.", "Close tube and shake for 2 min. by hand.", "Remove the swab and briefly centrifuge. •NOTE: The swab should be remo... |
null | null | null | dx.doi.org/10.17504/protocols.io.mesc3ee | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The basic imaging process for MERFISH involves several fluid handling steps, e.g. introduction of hybridization buffers and wash and imaging buffers, in conjunction with the collection of smFISH images and the photobleaching of the sample. While it would be possible to perfor... | [] |
34,989 | Denaturing RNA electrophoresis in urea polyacrylamide gels (Urea-PAGE) and semi-dry Northern Blot transfer | 1 | dx.doi.org/10.17504/protocols.io.beemjbc6 | https://www.protocols.io/view/denaturing-rna-electrophoresis-in-urea-polyacrylam-beemjbc6 | Alice Pawlowski | TITLE: Denaturing RNA electrophoresis in urea polyacrylamide gels (Urea-PAGE) and semi-dry Northern Blot transfer
AUTHORS: Alice Pawlowski
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">RNA is denatured in 8 M Urea and separated in a polyacrylamide g... | ["[preparation of Urea-PAA gel]\nassemble the gel sandwich stack (see pdf in materials section); align plate bottoms with the spacers on a flat surface before sliding the sandwich in the casting clamp assembly and tithening the screws (finger tight to prevent breaking of the plates)place the sandwich into the caster, ... |
54,688 | Depression Detection with DM | 3 | dx.doi.org/10.17504/protocols.io.bzm8p49w | https://www.protocols.io/view/depression-detection-with-dm-bzm8p49w | Umme Marzia Haque | TITLE: Depression Detection with DM
AUTHORS: Umme Marzia Haque
[DESCRIPTION]
The study has used data from YMM. The Yes/No variables that had a low correlation with target variable have been removed. To extract the most relevant features , the high correlated variables with the target variable , the Boruta method was ... | [] |
57,872 | Explant Surgery: Chronic recoverable Neuropixels in mice | 1 | null | https://www.protocols.io/view/explant-surgery-chronic-recoverable-neuropixels-in-b4rqqv5w | Emily A Aery Jones | TITLE: Explant Surgery: Chronic recoverable Neuropixels in mice
AUTHORS: Emily A Aery Jones
[DESCRIPTION]
This protocol collection explains how to build a low-cost, lightweight system to implant Neuropixels 1.0 probes into mice, record during freely moving behavior, then recover the probe for future use. This protocol... | ["[Prepare mouse] Set the nosecone to the position you recorded from the implant surgery. Attach mouse to headbar stereotactic adapters.", "[Remove probe] Remove all the tape to expose the screws and probe.", "[Remove probe] Cut the ground wire above the pin.", "[Remove probe] Attach the Sensapex holder and set the ste... |
null | null | null | dx.doi.org/10.17504/protocols.io.e9cbh2w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>CasPASE™ Apoptosis Fluorometric Assay provides a simple method for assaying the activities of various caspases (proteases), a key early indicator of apoptosis in mammalian cells. </p>
[BEFORE_START]
<p><em><strong>Preparation of Kit Reagents </strong> </em></p>
<p>1. Allow ... | [] |
95,877 | Singleplex Assay for Fitness Measurements | 0 | dx.doi.org/10.17504/protocols.io.8epv5xye4g1b/v1 | https://www.protocols.io/view/singleplex-assay-for-fitness-measurements-c9vdz626 | David Ross | TITLE: Singleplex Assay for Fitness Measurements
AUTHORS: David Ross
[DESCRIPTION]
This protocol outlines an assay for the singleplex measurement the fitness of plasmid variants in E.coli.
This protocol is used during onboarding of a new protein function for the Pooled, Growth-Based Assay. In this protocol, clonal b... | ["[Culture Preparation] Use a scraping from the glycerol stock for each clonal variant to start 5 mL cultures in M9 media, each in a 15 mL snap-cap culture tube.", "[Prepare the automation system or liquid handler] Load required reagents (M9 media, additives, antibiotics, etc.) and growth-plates into automation system ... |
47,810 | General bacteria and archaea 16S-rRNA (515Fmod-806Rmod) for Illumina amplicon sequencing | 1 | dx.doi.org/10.17504/protocols.io.bsxanfie | https://www.protocols.io/view/general-bacteria-and-archaea-16s-rrna-515fmod-806r-bsxanfie | Roey Angel, Eva Petrova | TITLE: General bacteria and archaea 16S-rRNA (515Fmod-806Rmod) for Illumina amplicon sequencing
AUTHORS: Roey Angel, Eva Petrova
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Universal 16S rRNA probe-based-qPCR assay for bacteria.</div><div class = "text-block"><span>The primers target the V4 regi... | ["[Primers]\nABCD1NameDirectionSequence1Target region22515Fmod_CS1ForwardACA CTG ACG ACA TGG TTC TAC AGT GYC AGC MGC CGC CGT AA515-5333806Rmod_CS2ReverseTAC GGT AGC AGA GAC TTG GTC TGG ACT ACN VGG GTW TCT AAT787-8061. CS + primer sequence (in bold)2. Relative to E. coli SSU rRNA gene\nABCD1NameDirectionSequence1Target ... |
101,541 | 12S PCR Metabarcoding Protocol for Fish Detection in Estuarine Samples | 4 | dx.doi.org/10.17504/protocols.io.3byl49wqogo5/v1 | https://www.protocols.io/view/12s-pcr-metabarcoding-protocol-for-fish-detection-dfed3ja6 | Fouad El Baidouri, Heather L. Gilbert, Alison Watts | TITLE: 12S PCR Metabarcoding Protocol for Fish Detection in Estuarine Samples
AUTHORS: Fouad El Baidouri, Heather L. Gilbert, Alison Watts
[DESCRIPTION]
This methodology has been developed for the use of MiFish-U and MiFish-E primers multiplexed with Platinum SuperFi II (2X) and Zymo clean up under a touchdown PCR pro... | ["[DNA clean up using Zymo] Before setting up the PCR mix, purify the DNA using the Zymo OneStep PCR Inhibitor Removal Kit as indicated by the manufacturer (CAT: D6030 or D6035). After the clean up, dilute the DNA at a 1:5 ratio. Follow the the Guidelines & Warnings for lab space preparation and clean up. See Descripti... |
83,838 | FIVTools Thresholding and Measures | 5 | dx.doi.org/10.17504/protocols.io.e6nvwd6mdlmk/v1 | https://www.protocols.click/view/fivtools-thresholding-and-measures-cv46w8ze | d.grigore | TITLE: FIVTools Thresholding and Measures
AUTHORS: d.grigore
[DESCRIPTION]
YouTube: FIVTools Thresholding and Measures Walkthrough
The goal is to set a criteria on a single FOV for machine learning that will ultimately lead to a final output of single cells with associated intracellular metrics.
[STEPS]
SECTION: Nav... | ["[Navigating FIVTools Image GUI] Open the FIVTools application.", "[Navigating FIVTools Image GUI] Access recent scans by navigating to the correct directory under which the images are saved under \"InCell Images dB (Root). Click the \"Refresh\" button to update the directory of images.", "[Navigating FIVTools Image G... |
80,304 | TSS competent cells and transformation | 4 | dx.doi.org/10.17504/protocols.io.5jyl8jzbrg2w/v1 | https://www.protocols.click/view/tss-competent-cells-and-transformation-csnqwddw | David Ruano Gallego | TITLE: TSS competent cells and transformation
AUTHORS: David Ruano Gallego
[DESCRIPTION]
This protocol allows the efficient transformation of plasmid into E. coli and related strains.
[BEFORE_START]
Prepare solution TSS and KCM.
When prepared, TSS has a cloudy appearance that disappears once autoclaved. Also, over ... | ["[Preparing competent cells] Grow o/n a 5 mL culture of the strain to be transformed.\n5 mL 37 °C", "[Preparing competent cells] Dilute 1:100 of the preinoculum in 50 mL prewarmed LB (OD600 = 0,05).\n50 mL 37 °C", "[Preparing competent cells] Grow at 37ºC with agitation (170 rpm) until it reaches OD600 = 0,5.", "[Prep... |
94,806 | Differential transcript usage analysis | 4 | dx.doi.org/10.17504/protocols.io.261gedmjdv47/v1 | https://www.protocols.io/view/differential-transcript-usage-analysis-c8twzwpe | Peter Kilfeather | TITLE: Differential transcript usage analysis
AUTHORS: Peter Kilfeather
[DESCRIPTION]
Differential transcript usage analysis from Kilfeather, Khoo et al., 2024
[STEPS]
SECTION: Protocol
1. Differential transcript usage was performed using DRIMSeq (v1.24.0). To consider only genes containing more than 1 isoform, with ... | ["[Protocol] Differential transcript usage was performed using DRIMSeq (v1.24.0). To consider only genes containing more than 1 isoform, with a minimum level of expression, the following parameters were used: min_samps_gene_expr = n * 0.75, min_gene_expr = 10, min_samps_feature_expr = 10, min_feature_expr = 10, min_sam... |
97,091 | USDA LTAR Common Experiment measurement: Post-processing of spatial grain yield data | 1 | dx.doi.org/10.17504/protocols.io.4r3l2qmy3l1y/v1 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-post-proce-da3b2gin | Olivia K. Owen, Kenneth A. Sudduth, Lori J. Abendroth | TITLE: USDA LTAR Common Experiment measurement: Post-processing of spatial grain yield data
AUTHORS: Olivia K. Owen, Kenneth A. Sudduth, Lori J. Abendroth
[DESCRIPTION]
This protocol provides general recommendations for in-field data collection and detailed methods for post-processing grain yield data. Grain yield is ... | ["[Harvester setup and calibration] To ensure the most accurate sensor readings and data collection, follow the calibration guidelines in the combine and yield monitor manuals.", "[Machine operation] Before operating any machinery, understand the basic safety and use protocols.", "[Machine operation] Take time to recog... |
99,873 | 0.9% Saline Solution | 1 | dx.doi.org/10.17504/protocols.io.kxygxe81zv8j/v2 | https://www.protocols.io/view/0-9-saline-solution-ddr92596 | Allen Institute for Brain Science | TITLE: 0.9% Saline Solution
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
This protocol is used to prepare 0.9% Saline Solution. 0.9% Sterile Saline solution is used as an initial rinse for the brains of mice prior to perfusion.
Note: Research reported in this publication was supported by the National Ins... | [] |
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