id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
|---|---|---|---|---|---|---|---|
34,908 | s3-GCC | 1 | dx.doi.org/10.17504/protocols.io.beb4jaqw | https://www.protocols.io/view/s3-gcc-beb4jaqw | Andrew Adey, Ryan Mulqueen | TITLE: s3-GCC
AUTHORS: Andrew Adey, Ryan Mulqueen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">s3GCC (symmetrical strand single-cell combinatorial indexing; genome conformation capture) protocol for the capture of Hi-C like chimeric reads, generated through proximity ligation, as well as s... | ["[Prepare oligoes and transposases]\nAttached is a s3 molecular layout per step. As well as oligonucleotides used in the protocol. Prior to starting, iTSM plate should be prepared following the protocols listed within this step.Tabs with oligonucleotides to be ordered and prepared:iTSM sci_WGA14_ME_LNAPCR_i5 primersPC... |
95,953 | Protocol SAM-Seq A.Thaliana | 4 | dx.doi.org/10.17504/protocols.io.8epv5x1xdg1b/v2 | https://www.protocols.io/view/protocol-sam-seq-a-thaliana-c9xrz7m6 | basile.leduque, Quadrana Leandro | TITLE: Protocol SAM-Seq A.Thaliana
AUTHORS: basile.leduque, Quadrana Leandro
[DESCRIPTION]
Background: Epigenetic modifications, including chromatin accessibility, nucleosome positioning, and DNA methylation (5mC), are pivotal in shaping genome function. However, current short read sequencing approaches present cha... | ["[Plant nuclei purification and permeabilization] Starting material: 1g of powder ( Aerial part of ~17 days seedling )\n\nAdd the powder to 25 ml of Extraction Buffer (EB) 1 in a 50 ml falcon tube. Let sit on ice for 5 min.", "[Plant nuclei purification and permeabilization] Add 1% Formaldehyde for crosslinking (i.e... |
20,474 | Characterization of iPSC | null | dx.doi.org/10.17504/protocols.io.x82frye | null | Celeste Karch, Rita Martinez, Jacob Marsh | TITLE: Characterization of iPSC
AUTHORS: Celeste Karch, Rita Martinez, Jacob Marsh
[STEPS]
?. Coat T25 flask, chamber slide and plates with Matrigel prior to passaging.
?. Aspirate media from cell culture.
?. Wash with PBS per well and aspirate.
1 ml
?. Add Accutase per well of 6 well plate.
1 ml
?. Incubate infor ... | ["Coat T25 flask, chamber slide and plates with Matrigel prior to passaging.", "Aspirate media from cell culture.", "Wash with PBS per well and aspirate.\n1 ml", "Add Accutase per well of 6 well plate.\n1 ml", "Incubate infor 3-4 minutes.", "Collect cells from 2 wells with DMEM/F12 per well and transfer into 15mL co... |
36,936 | Fig2 | 1 | dx.doi.org/10.17504/protocols.io.bgbgjsjw | https://www.protocols.io/view/fig2-bgbgjsjw | Runtian Zhang | TITLE: Fig2
AUTHORS: Runtian Zhang
[STEPS]
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.jh9cj96 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span style="font-weight: 400;">Esta prueba evalúa la actividad hemolítica de péptidos o moleculas sobre eritrocitos humanos como criterio de selectividad ante las células eucariotas. </span></p>
<p> </p>
<p><span style="font-weight: 400;">La realizacion de este protocolo fue... | [] |
68,132 | Tissue NET-seq (native elongating transcript sequencing) | 4 | dx.doi.org/10.17504/protocols.io.kxygxzj3dv8j/v1 | https://www.protocols.io/view/tissue-net-seq-native-elongating-transcript-sequen-cescteaw | Mihaela Bozukova, Peter Tessarz | TITLE: Tissue NET-seq (native elongating transcript sequencing)
AUTHORS: Mihaela Bozukova, Peter Tessarz
[DESCRIPTION]
Quantifying crucial steps in gene regulation during transcription elongation, such as promoter-proximal pausing, requires high resolution methods to map the transcription machinery across the genom... | ["[Tissue homogenization and cell fractionation] Place the fresh liver tissue in ice-cold PBS, cut into smaller pieces and homogenize in 3 ml of nuclei isolation buffer using a Dounce tissue homogenizer (Wheaton). After complete homogenization, transfer the sample to a Falcon tube. Add 3 ml of nuclei isolation buffer t... |
93,104 | Cyanobacteria growth (English) | 1 | dx.doi.org/10.17504/protocols.io.5jyl8pxqdg2w/v1 | https://www.protocols.io/view/cyanobacteria-growth-english-c66qzhdw | Ricardo M. Borges, Gabriela de Assis Ferreira, Fernanda Chagas, pauloihc | TITLE: Cyanobacteria growth (English)
AUTHORS: Ricardo M. Borges, Gabriela de Assis Ferreira, Fernanda Chagas, pauloihc
[DESCRIPTION]
This document aims to present a comprehensive protocol for the cultivation of cyanobacteria in saline aquatic environments. The protocol incorporates etailed information on the composit... | ["[Material] Preparation of F/2 Culture Medium:\nFor 1 liter of distilled water, add:\n41.5 grams of marine salt for aquarium (Reef Salt)\n1 mL of NaNO3 Stock Solution\n1 mL of NaH2PO4•H2O Stock Solution\n1 mL of Trace Metal Stock Solution\nAutoclave: 15 minutes at 121°C\nWait for the medium to cool down.\n\nAdd 0.5 mL... |
67,105 | Biological sample lysis and extraction using the Covaris LE220+ for LC-MS | 1 | dx.doi.org/10.17504/protocols.io.8epv598b6g1b/v1 | https://www.protocols.io/view/biological-sample-lysis-and-extraction-using-the-c-cdr9s596 | ronan.ocualain | TITLE: Biological sample lysis and extraction using the Covaris LE220+ for LC-MS
AUTHORS: ronan.ocualain
[DESCRIPTION]
This protocol details the procedure of sample lysis and protein extraction using the Covaris LE220+ ultrasonicator.
[BEFORE_START]
Initial preparation:
Before you begin:
Log onto the LE220+ and ch... | ["[Preparing samples and LE220+] You will need to dilute the S-Trap lysis buffer to a working stock concentration. \n \n\n\nUsing the 1mL pipette, add 500 µL of 2x stock of S-Trap lysis buffer to 500 µL of LCMS grade water.\nThis is the working stock concentration (5% SDS, 50 millimolar (mM) TEAB pH 7.5).", "[Preparing... |
64,841 | Attention! Condor CBD Gummies Reviews: Cost, Side Effects, Ingredients, Benefits, Where To Buy? | 3 | dx.doi.org/10.17504/protocols.io.3byl4b6r8vo5/v1 | https://www.protocols.io/view/attention-condor-cbd-gummies-reviews-cost-side-eff-cbjhskj6 | johnkikal | TITLE: Attention! Condor CBD Gummies Reviews: Cost, Side Effects, Ingredients, Benefits, Where To Buy?
AUTHORS: johnkikal
[DESCRIPTION]
Attention! Condor CBD Gummies Reviews: Cost, Side Effects, Ingredients, Benefits, Where To Buy?
[STEPS] | [] |
100,395 | Guide to molecular databases and molecular phylogenetics and evolution | 5 | dx.doi.org/10.17504/protocols.io.36wgq77e3vk5/v6 | https://www.protocols.io/view/guide-to-molecular-databases-and-molecular-phylog-deaj3acn | Florian G Jacques | TITLE: Guide to molecular databases and molecular phylogenetics and evolution
AUTHORS: Florian G Jacques
[DESCRIPTION]
Developments in sequencing technologies and the sequencing of an ever-increasing number of genomes have revolutionised studies into biodiversity and organismal evolution. This accumulation of data ha... | ["[Introduction] We provide a step-by-step protocol for non-bioinformatic users to reconstruct the phylogeny and evolutionary history of genes or proteins. \n\nWe present a compilation of DNA and protein databases, as well as bioinformatic tools for molecular phylogenetic reconstruction, and a wide range of studies on ... |
97,413 | Parse Evercode WT -- University of Minnesota TMCs | 0 | dx.doi.org/10.17504/protocols.io.dm6gpz8rplzp/v1 | https://www.protocols.io/view/parse-evercode-wt-university-of-minnesota-tmcs-dbdd2i26 | Laura J Niedernhofer, David A Bernlohr | TITLE: Parse Evercode WT -- University of Minnesota TMCs
AUTHORS: Laura J Niedernhofer, David A Bernlohr
[DESCRIPTION]
Combinatorial barcoding is an instrument-free method used to generate single-cell and single-nucleus RNA-sequencing libraries. Outlined here are the methods used to isolate single-nuclei from frozen t... | ["[Single Nuclei Dissociation]", "[Nuclei Fixation Protocol]", "[Evercode WT Protocol]", "[FASTQ Generation] BCL data from Illumina sequencer is demultiplexed and converted into FASTQ format using bcl2fastq version 2.20.0"] |
87,610 | Overloading and Unpacking (OAK) V1 | 4 | dx.doi.org/10.17504/protocols.io.6qpvr39opvmk/v1 | https://www.protocols.io/view/overloading-and-unpacking-oak-v1-czs2x6ge | Hayley Bennett, Bing Wu | TITLE: Overloading and Unpacking (OAK) V1
AUTHORS: Hayley Bennett, Bing Wu
[DESCRIPTION]
High-throughput single-cell sequencing is a powerful technique for investigating the cellular diversity of complex biological systems. Throughput, cost effectiveness, and experimental simplicity are crucial forefronts of technolog... | ["[Before starting] Ensure you have adequate aliquots of methanol at -20 °C . We recommend storing in a cool block that can be pulled out and taken to a fume hood for use.\n\nPrepare your single-cell suspension. We recommend starting with over 500K cells if possible, to ensure a visible pellet after fixation.\n\nPre-co... |
70,635 | Matrix Sublimation via In-House Developed Sublimation Apparatus | 1 | dx.doi.org/10.17504/protocols.io.n92ldpyn9l5b/v1 | https://www.protocols.io/view/matrix-sublimation-via-in-house-developed-sublimat-cg8jtzun | David Anderson, Martin Dufresne, Jeffrey D. Messinger, Jamie Allen, Katerina V Djambazova, Angela Kruse, Christine A. Curcio, Kevin L. Schey, Richard M. Caprioli, Jeff Spraggins | TITLE: Matrix Sublimation via In-House Developed Sublimation Apparatus
AUTHORS: David Anderson, Martin Dufresne, Jeffrey D. Messinger, Jamie Allen, Katerina V Djambazova, Angela Kruse, Christine A. Curcio, Kevin L. Schey, Richard M. Caprioli, Jeff Spraggins
[DESCRIPTION]
Scope:
This protocol describes the use o... | ["[Matrix Preparation] Dissolve 5mg of matrix in 2 mL of acetone for a total matrix concentration of 2.5 mg/mL.", "[Sublimation Procedure] Place apparatus lid on the base, ensure that base is fully seated in the indentions on the induction heater top cover. Start pulling vacuum.", "[Sublimation Procedure] Once it is cl... |
71,238 | Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons | 1 | dx.doi.org/10.17504/protocols.io.e6nvwj54dlmk/v1 | https://www.protocols.io/view/piggybac-mediated-stable-expression-of-ngn2-in-ips-chtet6je | Dan Dou, C. Alexander Boecker, Erika L.F. Holzbaur | TITLE: Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons
AUTHORS: Dan Dou, C. Alexander Boecker, Erika L.F. Holzbaur
[DESCRIPTION]
We adapted a previously-described method (Pantazis et al., 2022) for employing Piggybac transfection to stably express doxycycl... | ["[Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons] Culture iPSCs in a 10 cm dish coated with Growth Factor Reduced Matrigel (Corning) and feed daily with Essential 8 media (ThermoFisher).", "[Piggybac-mediated stable expression of NGN2 in iPSCs for differe... |
82,333 | Cell counting, imaging, and analysis | 3 | dx.doi.org/10.17504/protocols.io.81wgbyjjqvpk/v1 | https://www.protocols.io/view/cell-counting-imaging-and-analysis-cum5wu86 | Shiyi Wang | TITLE: Cell counting, imaging, and analysis
AUTHORS: Shiyi Wang
[DESCRIPTION]
Cell counting, imaging, and analysis
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ka6cshe | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
55,392 | Distillation of total reduced inorganic sulfur from marine sediments | 6 | dx.doi.org/10.17504/protocols.io.b2b8qarw | https://www.protocols.io/view/distillation-of-total-reduced-inorganic-sulfur-fro-b2b8qarw | Patrick D Larkin, Sebastian Rubiano-Rincon | TITLE: Distillation of total reduced inorganic sulfur from marine sediments
AUTHORS: Patrick D Larkin, Sebastian Rubiano-Rincon
[DESCRIPTION]
This procedure can be used to precipitate H2S as Ag2S from the Total Reduced Inorganic Sulfur (TRIS) fraction of marine sediments.
[GUIDELINES]
This procedure takes 6 - 8 hou... | ["[Distillation Apparatus Set-Up] Connect a 250 ml Buffer (Gas washing) Bottle (D) to the Connection Adapter for Condenser (C). Attach the glassware to the framework with clamps (not shown):", "[Distillation Apparatus Set-Up] Insert a Trap Orifice Tube (F) into one of a Smog Bubbler Trap Bottle (E).Insert a Trap Fritte... |
36,396 | VU Biomolecular Multimodal Imaging Center (BIOMIC) kidney characterization pipeline for tissues collected through the Cooperative Human Tissue Network (CHTN) | null | dx.doi.org/10.17504/protocols.io.bfskjncw | https://www.protocols.io/view/vu-biomolecular-multimodal-imaging-center-biomic-k-bfskjncw | Elizabeth Neumann, Jamie Allen, Maya Brewer, David Anderson, Mark De Caestecker, Danielle Gutierrez, Jeff Spraggins | TITLE: VU Biomolecular Multimodal Imaging Center (BIOMIC) kidney characterization pipeline for tissues collected through the Cooperative Human Tissue Network (CHTN)
AUTHORS: Elizabeth Neumann, Jamie Allen, Maya Brewer, David Anderson, Mark De Caestecker, Danielle Gutierrez, Jeff Spraggins
[DESCRIPTION]
<div class = "t... | ["Collection of post-surgical tissue.Collection: dx.doi.org/10.17504/protocols.io.7gehjte", "Stabilize and freeze tissues.Freezing Tissue: dx.doi.org/10.17504/protocols.io.6wghfbw", "Intial Rapid Pathology Assessment of Kidney TissueStaining: dx.doi.org/10.17504/protocols.io.4qngvveAssessment: dx.doi.org/10.17504/pro... |
42,655 | TUNEL | 4 | null | https://www.protocols.io/view/tunel-bmv7k69n | 张 雪 | TITLE: TUNEL
AUTHORS: 张 雪
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Day1]
复水：75%MetOH+25%PBT 1ml shake for 5min
?. [Day1]
50%MetOH+50%PBT 5min
?. [Day1]
25%MetOH+75%PBT 5min
?. [Day1]
100%PBT 5min 1/4
融化PK
?. [Day1]
100%PBT 5min 2/4
?. [Day1]
100%PBT 5min 3/4
?. [Day1]
100%PBT 5min 4/4
?. [Day1]
透化：... | ["[Day1]\n复水：75%MetOH+25%PBT 1ml shake for 5min", "[Day1]\n50%MetOH+50%PBT 5min", "[Day1]\n25%MetOH+75%PBT 5min", "[Day1]\n100%PBT 5min 1/4\n融化PK", "[Day1]\n100%PBT 5min 2/4", "[Day1]\n100%PBT 5min 3/4", "[Day1]\n100%PBT 5min 4/4", "[Day1]\n透化：Proteinase K: PBT = 1 : 2000 (终浓度5µg/ml），5min", "[Day1]\n再固定：4%PFA 20min\n加P... |
18,041 | Simple 3D imaging of biogenic silica structures by fluorescence microscopy | null | dx.doi.org/10.17504/protocols.io.vuze6x6 | null | Sebastien COLIN | TITLE: Simple 3D imaging of biogenic silica structures by fluorescence microscopy
AUTHORS: Sebastien COLIN
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes how to prepare, stain and mount </span><a style = "text-decoration:underline;color:blue;cursor:pointer;"><span styl... | ["Preparation of the biogenic silica particles. If they come from living organisms, they should be cleaned and the organic materials removed.There are many protocols available in the literature which are based on acidic and/or oxidizing and/or detergent and/or solvent treatments.The mineral micro-fossils or sediments c... |
64,361 | Melt-Away Keto: SCAM ALERT 2022? READ MY EXPERIENCE! | 3 | dx.doi.org/10.17504/protocols.io.dm6gpbq11lzp/v1 | https://www.protocols.io/view/melt-away-keto-scam-alert-2022-read-my-experience-ca4hsgt6 | H H | TITLE: Melt-Away Keto: SCAM ALERT 2022? READ MY EXPERIENCE!
AUTHORS: H H
[DESCRIPTION]
you have the real factors you really want. This treatment is the most secure, most dependable weight reduction supplement accessible anyplace. In addition, it's likewise the most reasonable.
[STEPS] | [] |
40,866 | Publication and Data Sharing Policy (Part 13 of Phase 3 study of Vaccine Candidate for COVID-19) | 1 | dx.doi.org/10.17504/protocols.io.bj6akrae | https://www.protocols.io/view/publication-and-data-sharing-policy-part-13-of-pha-bj6akrae | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | TITLE: Publication and Data Sharing Policy (Part 13 of Phase 3 study of Vaccine Candidate for COVID-19)
AUTHORS: Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is Part 13 of "Phase 3 randomized, double-blinded, placebo-controlled tri... | [] |
58,783 | Indoor active search for adult Aedes sp. and Culex sp. mosquitoes | 2 | dx.doi.org/10.17504/protocols.io.b5m7q49n | https://www.protocols.io/view/indoor-active-search-for-adult-aedes-sp-and-culex-b5m7q49n | Erika Santamaria, Paola Muñoz, Catalina Marceló | TITLE: Indoor active search for adult Aedes sp. and Culex sp. mosquitoes
AUTHORS: Erika Santamaria, Paola Muñoz, Catalina Marceló
[DESCRIPTION]
This protocol details the indoor active search carried out in the research project: "Spatial stratification of dengue based on the identification of risk factors: a pilot tr... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.efqbbmw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol provides a method for identifying bacteriophage/virus taxonomy without a virome dataset using UniProt reference database. Based on the methods found in the following publication:<br /><br />Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: T... | [] |
102,038 | ALGAE DNA COLLECTION PROTOCOL | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1e52lmk/v1 | https://www.protocols.io/view/algae-dna-collection-protocol-dfvw3n7e | susannat | TITLE: ALGAE DNA COLLECTION PROTOCOL
AUTHORS: susannat
[DESCRIPTION]
This protocol details the collection, filtering, and preservation of algae samples for DNA sequencing. Using 47mm Whatman/Swinnex filter holders or a filter funnel with 0.45µm cellulose nitrate filters, algae are captured from a homogenized bioassess... | ["[Sample collection] The sample for DNA sequencing will be sourced from the bioassessment composite bucket. To separate sample, mix bioassessment composite bucket to re-suspend any settled particles, and allow sand and other large particles to briefly settle. Pour entire supernatant into a 1L bottle.", "[Sample collec... |
49,884 | DNA extraction - CTAB - eppendorf-scale | 4 | null | https://www.protocols.io/view/dna-extraction-ctab-eppendorf-scale-bux4nxqw | Simon Joly | TITLE: DNA extraction - CTAB - eppendorf-scale
AUTHORS: Simon Joly
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">DNA extraction - CTAB - eppendorf-scale</div><div class = "text-block"><span style = "font-style:italic;">Julian Starr and Simon Joly; September 2002</span></div></div>
[STEPS]
?. [Pre... | ["[Preparation]\nGeneral PreparationTo clean pestels (eppendorf-size pestel), wash well, rinse with 100% ETOH and with distilled water. Then autoclave them to avoid contamination.Label 3 sets of 1.5 ml eppendorf tubes. A maximum of 42 samples (=spaces in centrifuges) are possible in one day but this requires two people... |
29,197 | IHC-Amplified Fluorescent Frozen Sections | null | dx.doi.org/10.17504/protocols.io.8rmhv46 | null | Elizabeth Smith | TITLE: IHC-Amplified Fluorescent Frozen Sections
AUTHORS: Elizabeth Smith
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Immunohistochemistry protocol used for staining with fluorescent secondary antibodies to highlight specific tissue structures and amplify specific antibody signals.</div></div>
... | ["Day 1: Using the PAP Pen, carefully draw a water barrier circle around the tissue sections on the slide – allow this circle to dry for several seconds or up to approx. one minute", "Rinse slides with PBS (pH 7.3-7.4): 4 x 5 min each", "Rinse slides with 0.5% BSA + 0.4% Triton X-100 in PBS): 1 x 10 min", "Remove slid... |
82,916 | Expression and purification of PI3KC3-C1 complex | 1 | dx.doi.org/10.17504/protocols.io.5qpvorjezv4o/v1 | https://www.protocols.io/view/expression-and-purification-of-pi3kc3-c1-complex-cu8cwzsw | Minghao Chen, Xuefeng Ren | TITLE: Expression and purification of PI3KC3-C1 complex
AUTHORS: Minghao Chen, Xuefeng Ren
[DESCRIPTION]
Expression and purification of PI3KC3-C1 complex
[STEPS]
SECTION: Expression
1. Transfect HEK GNTi cells at concentration of 2 × 10^6 cells/ml
SECTION: Expression
2. Dilute PEI with Warm Hybridoma-SFM(1X)
SECTION:... | ["[Expression] Transfect HEK GNTi cells at concentration of 2 × 10^6 cells/ml", "[Expression] Dilute PEI with Warm Hybridoma-SFM(1X)", "[Expression] In a separate tube, dilute DNA with Hybridoma-SFM(1X)", "[Expression] Add PEI to DNA dilution. Incubate mixture for 30 min at 37 °C", "[Expression] Add mixture to cells. ... |
95,685 | LC-MS/MS analysis of 5 steroids in plasma in a clinical study of Congenital Adrenal Hyperplasia | 1 | null | https://www.protocols.io/view/lc-ms-ms-analysis-of-5-steroids-in-plasma-in-a-cli-c9pdz5i6 | Catriona Kyle, George Just, Scott G Denham, Natalie ZM Homer | TITLE: LC-MS/MS analysis of 5 steroids in plasma in a clinical study of Congenital Adrenal Hyperplasia
AUTHORS: Catriona Kyle, George Just, Scott G Denham, Natalie ZM Homer
[DESCRIPTION]
Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive disorders that affects adrenal steroidogenesis, resulting in ... | ["[Preparation of calibration standard stock solutions] Prepare 1 mg/mL stock solution of d8-corticosterone (d8B) from powder then prepare separate 100 µg/mL stock solutions of each steroid - d8B, cortisol (F), 17a-hydroxyprogesterone (17OHP4), testosterone (T) and androstenedione (A4) in methanol.", "[Preparation of c... |
87,390 | Sanger Tree of Life HMW DNA Extraction: Automated Plant MagAttract v.2 | 4 | dx.doi.org/10.17504/protocols.io.36wgq3n13lk5/v1 | https://www.protocols.io/view/sanger-tree-of-life-hmw-dna-extraction-automated-p-czj6x4re | Maja Todorovic, graeme oatley, Caroline Howard | TITLE: Sanger Tree of Life HMW DNA Extraction: Automated Plant MagAttract v.2
AUTHORS: Maja Todorovic, graeme oatley, Caroline Howard
[DESCRIPTION]
This protocol describes the automated extraction of HMW DNA from cryogenically homogenised tissue samples from plants and fungi intended for long-read sequencing. It emplo... | ["[Sample lysis] Prepare a lysis buffer master mix:\n ReagentVolume per samplePhosphate buffered saline (PBS)200 µLProteinase K20 µLRNase A4 µLBuffer AL150 µL", "[Sample lysis] Set a heat block to 55 °C.", "[Sample lysis] Transfer 50 mg of cryogenically homogenised tissue from each sample to 2 mL microcentrifuge tubes... |
77,142 | High-Throughput RNA Extraction on Agilent Bravo | 1 | dx.doi.org/10.17504/protocols.io.36wgqjex3vk5/v1 | https://www.protocols.io/view/high-throughput-rna-extraction-on-agilent-bravo-cpjwvkpe | Miriam R Simon, Matt S Zinter, Joseph Derisi | TITLE: High-Throughput RNA Extraction on Agilent Bravo
AUTHORS: Miriam R Simon, Matt S Zinter, Joseph Derisi
[DESCRIPTION]
Extracting bulk-RNA from biospecimens is a time-consuming process. Column-based extractions performed by hand must be done in batches and the number of samples that can be extracted per batch is d... | ["[Same Day Reagent Preparation] Plate and seal all reagents in the biosafety cabinet on the same day you plan to run the procedure with the following volumes:\n \nReagentVolume per wellQuantityPlateLysis Buffer630 uL11 mL deep-wellMagbeads35 uL1Biorad 96-well PCREthanol900 uL11 mL deep-wellEthanol600 uL41 mL deep-well... |
null | null | null | dx.doi.org/10.17504/protocols.io.deq3dv | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<strong>SN Media Recipe</strong><br /><br />1. Pour a 75% Seawater mixture (ie. for <strong>1L</strong>, add<strong> 750ml White Point Seawater</strong> and <strong>250ml MQ Water</strong>) into a clean, capped bottle. Autoclave using the p18 liquid cyle (NOTE: This should take ... | [] |
67,456 | Diaetovita Erfahrungen Eine brillante Gewichtsabnahme-Ergänzung | Deutschlands beste Gewichtsverlustpillen | 1 | dx.doi.org/10.17504/protocols.io.kxygxz6e4v8j/v1 | https://www.protocols.io/view/diaetovita-erfahrungen-eine-brillante-gewichtsabna-cd48s8zw | polbatra | TITLE: Diaetovita Erfahrungen Eine brillante Gewichtsabnahme-Ergänzung | Deutschlands beste Gewichtsverlustpillen
AUTHORS: polbatra
[DESCRIPTION]
Wenn Sie überschüssiges Körperfett verlieren und in nur wenigen Wochen eine fitte Körperform erreichen möchten, müssen Sie dieses Medikament, das in Form von winzigen Tabl... | ["[Diaetovita Erfahrungen Eine brillante Gewichtsabnahme-Ergänzung | Deutschlands beste Gewichtsverlustpillen]"] |
48,934 | publication 3 | 1 | null | https://www.protocols.io/view/publication-3-bt2enqbe | Mariia Guliakina | TITLE: publication 3
AUTHORS: Mariia Guliakina
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Sudden she seeing garret far regard. By hardly it direct if pretty up regret. Ability thought enquire settled prudent you sir. Or easy knew sold on well come year. Something consulted age extremely end pro... | ["test", "est 2", "test 3"] |
63,823 | Qubit DNA Quantification (Assay) | 4 | null | https://www.protocols.io/view/qubit-dna-quantification-assay-cajpscmn | Allyson Hirsch, George Testo | TITLE: Qubit DNA Quantification (Assay)
AUTHORS: Allyson Hirsch, George Testo
[DESCRIPTION]
Achieve accurate and precise quantification of dsDNA with Qubit dsDNA HS (High Sensitivity) and Qubit dsDNA BR (Broad Range) Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high abu... | ["[Preparing Working Solution + Samples] Clean the workstation with DNA Away and Ethanol.", "[Preparing Working Solution + Samples] Remove the PCR plates from the -20 °Cfreezer to thaw.", "[Preparing Working Solution + Samples] Prepare two assay tubes for the upper and lower bound standards, and also one tube for each ... |
43,107 | Affinity purification of ookinetes in Petri dishes | 4 | dx.doi.org/10.17504/protocols.io.bncbmasn | https://www.protocols.io/view/affinity-purification-of-ookinetes-in-petri-dishes-bncbmasn | Benito Recio Totoro | TITLE: Affinity purification of ookinetes in Petri dishes
AUTHORS: Benito Recio Totoro
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The ookinetes, as they come from the ookinete culture, are suspended in a solution that also contains blood cells and other parasite stages. Often, it is necessary t... | ["[Ookinete purification]\nThaw the ECM gel overnight at in ice\n4 °C\nKeep the ECM gel in ice at all times. ECM gel starts to form a gel at 20°C", "[Ookinete purification]\nDilute the ECM gel 1:2 or 1:3 with cold ECM buffer, mix well and coat the Petri dishes with .Allow gel formation at for .\n0.5 mL\n37 °C\nUp to... |
null | null | null | dx.doi.org/10.17504/protocols.io.mkzc4x6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kfictke | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Adapted from http://merenlab.org/2016/06/22/anvio-tutorial-v2/</p>
[BEFORE_START]
<p>The goal of this tutorial is to provide a brief overview of the anvi’o workflow for the analysis of assembly-based shotgun metagenomic data. Throughout this tutorial you will primarily learn... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.u63ezgn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
SECTION: Fix parameters, upload and create issue
?.
SECTION: Pull onto hazel hen, adapt submit script
?.
SECTION: Get simulation results into git annex
?.
SECTION: Do postprocessing
?.
SECTION: Submit
?. | ["[Fix parameters, upload and create issue] {\"blocks\":[{\"key\":\"dhdkt\",\"text\":\"Define a scenario (or more that belong together) and push it as a parameters file into https:\\/\\/simsgs.informatik.uni-stuttgart.de:8444\\/EXAHD\\/ParameterFiles\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entity... |
73,483 | Fluorescently labeled polyamine uptake (via Flow Cytometry) | 1 | dx.doi.org/10.17504/protocols.io.n92ldp8qxl5b/v1 | https://www.protocols.io/view/fluorescently-labeled-polyamine-uptake-via-flow-cy-cjzjup4n | Marine Houdou, Peter Vangheluwe | TITLE: Fluorescently labeled polyamine uptake (via Flow Cytometry)
AUTHORS: Marine Houdou, Peter Vangheluwe
[DESCRIPTION]
Assess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensitiy acquisition via flow cytometry.
[BEFORE_START]
P... | ["The day of the experiment, remove cell culture medium and add fluorescently labeled polyamines to the cells in a final volume of 500 µL.", "Incubate the desired time at 37°C, 5% CO2, protected from the light.", "Discard medium.", "Wash with 500 µL Versene or DPBS (-/-).", "Add 500 µL 1%-Albumin Fraction V in DPBS (-/... |
107,450 | AN OBSERVATIONAL STUDY ON THE EFFICACY OF DAPAGLIFLOZIN IN HEART FAILURE WITH REDUCED EJECTION FRACTION IN TERTIARY CARE HOSPITAL IN CENTRAL INDIA | 0 | dx.doi.org/10.17504/protocols.io.rm7vzjr18lx1/v1 | https://www.protocols.io/view/an-observational-study-on-the-efficacy-of-dapaglif-dk624zge | Meghna Bordoloi, Shilpa Bawankule, Sourya Acharya, Sunil Kumar | TITLE: AN OBSERVATIONAL STUDY ON THE EFFICACY OF DAPAGLIFLOZIN IN HEART FAILURE WITH REDUCED EJECTION FRACTION IN TERTIARY CARE HOSPITAL IN CENTRAL INDIA
AUTHORS: Meghna Bordoloi, Shilpa Bawankule, Sourya Acharya, Sunil Kumar
[DESCRIPTION]
Heart failure with reduced ejection fraction is a prevalent and debilitating co... | [] |
63,834 | Eagle Hemp CBD Gummies Reviews & Price For Sale – Easy To Use! | 1 | dx.doi.org/10.17504/protocols.io.6qpvr62nzvmk/v1 | https://www.protocols.io/view/eagle-hemp-cbd-gummies-reviews-amp-price-for-sale-caj2scqe | viaketoreal | TITLE: Eagle Hemp CBD Gummies Reviews & Price For Sale – Easy To Use!
AUTHORS: viaketoreal
[DESCRIPTION]
Eagle Hemp CBD GummiesMight it be said that you are encountering conditions like apprehension, tenacious torture, or even just higher than typical sensations of tension? There is another thing calledEagle Hem... | [] |
87,125 | Qiagen QIAmp PowerFecal Pro extraction kit | 4 | dx.doi.org/10.17504/protocols.io.j8nlko8owv5r/v1 | https://www.protocols.io/view/qiagen-qiamp-powerfecal-pro-extraction-kit-czbvx2n6 | Michael Dan Siemon, Dilan Joseph, Jessica Pardy, Richard Gibson, christopher.degroot | TITLE: Qiagen QIAmp PowerFecal Pro extraction kit
AUTHORS: Michael Dan Siemon, Dilan Joseph, Jessica Pardy, Richard Gibson, christopher.degroot
[DESCRIPTION]
This protocol is used by Western University to process wastewater samples for wastewater-based epidemiology. The protocol is modified from the protocol described... | ["[Sample Preparation] Thoroughly mix wastewater sample then aliquot 40 mL into 50 mL Falcon tube. Centrifuge at 4500 x g for 120 min. Decant supernatant, assume 280 µl pellet.", "[Sample Preparation] As a substitute for vortexing described in the kit protocol, bead-beating is used. Bead-beating is conducted 4x for 30s... |
64,456 | Eagle Hemp CBD Gummies - Latest Reviews + User Experience | 1 | dx.doi.org/10.17504/protocols.io.kxygxzq64v8j/v1 | https://www.protocols.io/view/eagle-hemp-cbd-gummies-latest-reviews-user-experie-ca7gshjw | oehrollerbuy | TITLE: Eagle Hemp CBD Gummies - Latest Reviews + User Experience
AUTHORS: oehrollerbuy
[DESCRIPTION]
On the off chance that you are one of the many individuals who needs to add CBD to their everyday daily practice, there is another item calledEagle Hemp CBD Gummiesthat you might believe should arrange at the earlie... | [] |
28,022 | MiniCircle Production | null | dx.doi.org/10.17504/protocols.io.7kwhkxe | https://www.protocols.io/view/minicircle-production-7kwhkxe | Eric Danner | TITLE: MiniCircle Production
AUTHORS: Eric Danner
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a cheap and efficent way to create Minicircles.</div><div class = "text-block"><span>This MiniCircle System was originally published: </span><a href="https://www.ncbi.nlm.nih.gov/entrez/eutils/e... | ["[Clone Stuff into your minicircle backbone]\nCloningThe backbone has a low-copy plasmid ORI. So you need to get a larger volume for mini-preps than usual. For sequencing one side of the multiple cloning sequence has a repetitive sequences for SecI cleavage and so can mess up the primer binding. You can Sanger Sequenc... |
49,877 | IPA assembly for PacBio Hifi reads | 5 | dx.doi.org/10.17504/protocols.io.buxvnxn6 | https://www.protocols.io/view/ipa-assembly-for-pacbio-hifi-reads-buxvnxn6 | a.kharabianmasouleh , priyanka.sharma , Othman Aldossary, Bader Alsubaie, Onkar Nath, Valentine Murigneux, Neena Mitter, Gabriel Rodrigues Alves Margarido, Bruce Topp, imssallem , Agnelo Furtado, Robert Henry | TITLE: IPA assembly for PacBio Hifi reads
AUTHORS: a.kharabianmasouleh , priyanka.sharma , Othman Aldossary, Bader Alsubaie, Onkar Nath, Valentine Murigneux, Neena Mitter, Gabriel Rodrigues Alves Margarido, Bruce Topp, imssallem , Agnelo Furtado, Robert Henry
[DESCRIPTION]
<div class = "text-blocks"><div class... | ["Steps:1) ccs.bam files were generated by PacBio Sequel II (Hifi) platform for all the plant species, used to assemble the genome using IPA tool.2) The IPA tool was installed using the conda environment, following the link:https://github.com/PacificBiosciences/pbipa"] |
75,545 | P3 VALORACIÓN PREINSCRIPCIONES | 1 | dx.doi.org/10.17504/protocols.io.ewov1o34klr2/v1 | https://www.protocols.io/view/p3-valoraci-n-preinscripciones-cmzzu776 | cgarcia | TITLE: P3 VALORACIÓN PREINSCRIPCIONES
AUTHORS: cgarcia
[DESCRIPTION]
Una vez admitida a trámite cada solicitud de admisión a los programas de doctorado de la EIDUCAM, seguirán el siguiente proceso para su valoración:
[STEPS]
SECTION: P3.-PROCEDIMIENTO DE VALORACIÓN DE PREINSCRIPCIONES Y RESOLUCIÓN DE ADMISIÓN
1. La ... | ["[P3.-PROCEDIMIENTO DE VALORACIÓN DE PREINSCRIPCIONES Y RESOLUCIÓN DE ADMISIÓN] La Secretaría de la EIDUCAM valorará administrativamente cada solicitud, estableciendo contacto con los candidatos cuando las solicitudes no cumplan con las exigencias documentales establecidas, estableciendo plazos para su subsanación en ... |
81,782 | S-2 SOIL PROCESSING | 4 | dx.doi.org/10.17504/protocols.io.6qpvr4j12gmk/v1 | https://www.protocols.io/view/s-2-soil-processing-ct4wwqxe | REDI-NET Consortium | TITLE: S-2 SOIL PROCESSING
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol details about soil processing.
[BEFORE_START]
BEFORE START
Pre-cool the Bullet Blender by adding dry ice into the cooling compartment and running the cooling program.
Clean the work surfaces with RNaseZap, then wipe the surfaces wi... | ["[1. SAMPLE LYSIS] Add 320 µL of 1x PBS and 80 µL ATL-DX Buffer to the bead tubes prepared on step 3 of Before Start section under the Guidelines & Warnings tab.", "[1. SAMPLE LYSIS] Use a clean spatula or forceps to weigh about 0.25 g soil sample and place it into each prepared bead tube. Record the weight.", "[1. SA... |
39,273 | PNGase F Protocol (Denaturing Conditions) | 1 | dx.doi.org/10.17504/protocols.io.bikhkct6 | https://www.protocols.io/view/pngase-f-protocol-denaturing-conditions-bikhkct6 | New England Biolabs | TITLE: PNGase F Protocol (Denaturing Conditions)
AUTHORS: New England Biolabs
[DESCRIPTION]
PNGase F is the most effective enzymatic method for removing almost allN-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose,... | ["[Denaturing Reaction Conditions:] Combine 1 µg - 20 µg, 1 µL and H2O (if necessary) to make a 10 µL total reaction volume.", "[Denaturing Reaction Conditions:] Denature glycoprotein by heating reaction at 100 °C for 10 min.", "[Denaturing Reaction Conditions:] Chill denatured glycoprotein on ice and centrifuge 10 s."... |
32,358 | MojoSort™ Mouse Ig light chain κ Nanobeads Column Protocol | null | dx.doi.org/10.17504/protocols.io.bbueinte | null | Sam Li | TITLE: MojoSort™ Mouse Ig light chain κ Nanobeads Column Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>BioLegend MojoSort™ Nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing in academic labs. T... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4 mL in a 5 mL (12 x 75 mm) polypropylene tube. Note: Keep MojoSort™ Buffer on ice throughout the procedure.", "Filter the cells wit... |
29,953 | Seeding | null | dx.doi.org/10.17504/protocols.io.9g9h3z6 | null | Francisco Javier Quero, Alfredo L´Homme Iriarte, Laura Armero | TITLE: Seeding
AUTHORS: Francisco Javier Quero, Alfredo L´Homme Iriarte, Laura Armero
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the procedures to seed the </span><span style = "font-style:italic;">Bacullis subtilis </span><span>inside the microfluidic chip to cre... | ["[Day before experiment]\nCellls from stock were streaked onto LB agar plate and incubated at 37 ºC overnight.", "[Day of the experiment]\nA single colony was picked from the plate and inoculated into 3 ml of LB broth in a 50 ml conical tube, and then incubated at 37 uC in a shaker.", "[Day of the experiment]\nAfter 2... |
53,810 | Microplate reader operating procedure | 4 | dx.doi.org/10.17504/protocols.io.bysspwee | https://www.protocols.io/view/microplate-reader-operating-procedure-bysspwee | Shuning Guo, Zhujun Wei | TITLE: Microplate reader operating procedure
AUTHORS: Shuning Guo, Zhujun Wei
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Use Tecan Spark® multimode microplate reader to measure absorbance or fluorescence intensity of green fluorescence protein.</div><br/></div>
[STEPS]
?. Turn on the power a... | ["Turn on the power and warm up for 10 minutes.", "Open the program and select \"New\" to create a new process.", "Select \"Absorbance\" in the left column. There is a schematic of a 96-well plate in the middle of the screen.", "You can select the hole to be used according to the spotting hole, and the selected hole wi... |
28,747 | Reverse transcription of RNA to cDNA | null | dx.doi.org/10.17504/protocols.io.8bjhskn | null | iGEM Dusseldorf | TITLE: Reverse transcription of RNA to cDNA
AUTHORS: iGEM Dusseldorf
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Synthesizing cDNA (for qPCR)</div></div>
[STEPS]
?. [Preparation of 1.33X yellow buffer]
33,25 µL 40x yellow sample buffer + 966,75 µL H2Ooptional: This was used to be able to see th... | ["[Preparation of 1.33X yellow buffer]\n33,25 µL 40x yellow sample buffer + 966,75 µL H2Ooptional: This was used to be able to see the pipetting scheme for qPCR more easily", "[Start]\nIn PCR stripes, pipet in the following order:100 ng template RNA1 µL random hexamer primersRNase-free H2O to 13 µL volume", "[Start]\nP... |
55,886 | Isolating and growing fibroblast cells from threespine stickleback (Gasterosteus aculeatus) | 4 | dx.doi.org/10.17504/protocols.io.b2tnqeme | https://www.protocols.io/view/isolating-and-growing-fibroblast-cells-from-threes-b2tnqeme | Gabrielle Q Le, Natalie Steinel, Lauren Fuess, Maria Rodgers, Daniel Bolnick | TITLE: Isolating and growing fibroblast cells from threespine stickleback (Gasterosteus aculeatus)
AUTHORS: Gabrielle Q Le, Natalie Steinel, Lauren Fuess, Maria Rodgers, Daniel Bolnick
[DESCRIPTION]
This protocol goes through the process of isolating and growing fibroblast cells from threespine stickleback.
[BEFO... | ["[Obtaining tissue] Prepare and sterile filter 30 mL of 5% FBS media.", "[Obtaining tissue] Prepare and sterile filter 10 mL of 20% FBS media.", "[Obtaining tissue] Prepare two petri dishes on ice for collecting the tissues. Fill the petri dish half of the way with the 5% FBS media.", "[Obtaining tissue] Prepare 2x 6 ... |
79,871 | Endogenous coimmunoprecipitation | 1 | dx.doi.org/10.17504/protocols.io.eq2ly7bqmlx9/v1 | https://www.protocols.io/view/endogenous-coimmunoprecipitation-cr87v9zn | michela.deleidi, Pascale Baden | TITLE: Endogenous coimmunoprecipitation
AUTHORS: michela.deleidi, Pascale Baden
[DESCRIPTION]
Protocol used for immunoprecipitation of HSP60 and LONP1 in HEK cells to show the interaction with V5-Flag-tagged WT-GCase
[STEPS]
7. Spin cells in a centrifuge at 250g for 5 minutes at room temperature.
8. Remove the super... | ["Spin cells in a centrifuge at 250g for 5 minutes at room temperature.", "Remove the supernatant and wash cells in PBS.", "Repeat steps 7 and 8 for a total of 2 washes.", "Lyse cells in 1% TBS + 0.5% NP40 + PI/PHI (Pierce #A32959)", "[Endogenous coimmunoprecipitation] Wash Protein G agarose fast-flow beads in TBS 1X +... |
101,540 | Assessing Fluoranthene Impact on Initiation of Feeding Response: A Time-to-Event Experiment with Parhyale hawaiensis | 0 | dx.doi.org/10.17504/protocols.io.5qpvok93zl4o/v1 | https://www.protocols.io/view/assessing-fluoranthene-impact-on-initiation-of-fee-dfec3jaw | Ibrahim Lawan | TITLE: Assessing Fluoranthene Impact on Initiation of Feeding Response: A Time-to-Event Experiment with Parhyale hawaiensis
AUTHORS: Ibrahim Lawan
[DESCRIPTION]
This protocol delineates a controlled laboratory experiment employing Parhyale hawaiensis, an amphipod crustacean, as a model organism to assess the influence... | ["[Introduction] Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants originating from diverse anthropogenic sources like oil spills and combustion processes (Diamond et al., 2003; Hylland, 2006). Fluoranthene, a notable PAH, has garnered attention due to its persistence, bioaccumulation pote... |
45,316 | 3.2 Nucleofection of iPSCs | 1 | dx.doi.org/10.17504/protocols.io.bqhcmt2w | https://www.protocols.io/view/3-2-nucleofection-of-ipscs-bqhcmt2w | Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp | TITLE: 3.2 Nucleofection of iPSCs
AUTHORS: Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is part 3.2 of the "</span><a href="https://www.protocols.io/private/C65DB81838BC11EBA61A0A58A9FEAC2A/protocols" style = "text-de... | ["[Nucleofection of iPSCs]\nIn order to electroporate piggyBac and transposase vectors into iPSCs in suspension, use the X-Unit of the 4D- Nucleofector™ System in combination with the P3 Primary Cell 4D-Nucleofector™ X Kit according to the manufacturer’s guidelines.", "[Nucleofection of iPSCs]\nFirst of all, prepare th... |
83,341 | Quality control assessment for microbial genomes: GalaxyTrakr MicroRunQC workflow | 1 | dx.doi.org/10.17504/protocols.io.5jyl8mj16g2w/v5 | https://www.protocols.click/view/quality-control-assessment-for-microbial-genomes-g-cvmmw446 | Ruth Timme, Yesha Shrestha, Tina.Pfefer, Paul Morin, Maria Balkey, Errol Strain | TITLE: Quality control assessment for microbial genomes: GalaxyTrakr MicroRunQC workflow
AUTHORS: Ruth Timme, Yesha Shrestha, Tina.Pfefer, Paul Morin, Maria Balkey, Errol Strain
[DESCRIPTION]
PURPOSE: Step-by-step instructions for checking WGS sequence quality for bacterial pathogens. The MicroRunQC workflow, implemen... | ["[Account set up] Create a GalaxyTrakr account here: https://account.galaxytrakr.org/Account/Register", "[Account set up] Log into your GalaxyTrakr account: https://galaxytrakr.org", "[Create a new history] Create a new history. \n\nWe recommend creating a new history for each new MiSeq Run and including the flow-cell... |
49,259 | DNA extraction protocol for DNA-metabarcoding of marine phytoplankton using Zymobiomics DNA minprep kit (Zymo Research; D4300) | 4 | dx.doi.org/10.17504/protocols.io.bucjnsun | https://www.protocols.io/view/dna-extraction-protocol-for-dna-metabarcoding-of-m-bucjnsun | Andersson Agneta, Bengt Karlson, Andersson F. Anders, Sonia Brugel, Latz Meike, Lycken Jenny, Mikael Hedblom, Anders Torstensson, Markus Lindh | TITLE: DNA extraction protocol for DNA-metabarcoding of marine phytoplankton using Zymobiomics DNA minprep kit (Zymo Research; D4300)
AUTHORS: Andersson Agneta, Bengt Karlson, Andersson F. Anders, Sonia Brugel, Latz Meike, Lycken Jenny, Mikael Hedblom, Anders Torstensson, Markus Lindh
[DESCRIPTION]
T... | ["[Sample/Filter collection of biomass at sea] Filter water samples onto 0.2 µm pore size Millipore filter (Merck Millipore; GSWP04700) attached to a filter funnel connected to a vacuum source. Filter a minimum of 500 ml collected seawater. \n\n500 mL", "[Laboratory preparations] Clean your work bench with 70% ethanol.... |
61,311 | PCR mixture and condition (2X SUPERGREEN PCR MASTER MIX) | 4 | dx.doi.org/10.17504/protocols.io.81wgb644qlpk/v2 | https://www.protocols.io/view/pcr-mixture-and-condition-2x-supergreen-pcr-master-b747rqzn | Yin-Tse Huang | TITLE: PCR mixture and condition (2X SUPERGREEN PCR MASTER MIX)
AUTHORS: Yin-Tse Huang
[DESCRIPTION]
PCR mixture and condition (2X SUPERGREEN PCR MASTER MIX)
[STEPS]
SECTION: For generic primers
1. PCR mixture PCR condition for generic primers
SECTION: For barcoded primers
3. PCR mixture for barcoded primers
... | ["[For generic primers] PCR mixture PCR condition for generic primers", "[For barcoded primers] PCR mixture for barcoded primers", "[For generic primers] PCR condition for generic primers", "[For barcoded primers] PCR condition for barcoded primers"] |
63,943 | Via Keto Gummies Amazon | 3 | dx.doi.org/10.17504/protocols.io.4r3l2opmqv1y/v1 | https://www.protocols.io/view/via-keto-gummies-amazon-capfsdjn | viaketogummiesus | TITLE: Via Keto Gummies Amazon
AUTHORS: viaketogummiesus
[DESCRIPTION]
Via Keto Gummies Amazon is a general enhancement that demonstrations to treat joint issues, strong torments, ligament agony, headaches, and body hurts in your body. It works quickly by delivering strong synthetic compounds that work to reestablis... | [] |
31,494 | High throughput pipette-tip hydroponics for collecting salt stress samples for RNA-seq | null | dx.doi.org/10.17504/protocols.io.bazeif3e | null | Magdalena Julkowska | TITLE: High throughput pipette-tip hydroponics for collecting salt stress samples for RNA-seq
AUTHORS: Magdalena Julkowska
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Growing on plants in hydroponics can take a lot of space, and thus failure to germinate is a risk that is usually taken into ... | ["[Prepare seeds]\nSterilize the Arabidopsis seeds (no more than +/- 400 seeds) in 2ml Eppendorf tube with 1 ml of 50% household bleach (diluted with MQ water) - keep for 10-20 minutes", "[Prepare seeds]\nWash the seeds with 2 ml of sterile MQ water - 5 to 8 times - preferably in the laminar hood", "[Prepare seeds]\nIn... |
13,957 | The PIRASOA programme: design, structure, organisation and indicators of a comprehensive regional Institutional Programme for the Prevention and Control of Healthcare-associated Infections and Antimicrobial Stewardship for hospitals and primary care settin | 1 | dx.doi.org/10.17504/protocols.io.rvdd626 | https://www.protocols.io/view/the-pirasoa-programme-design-structure-organisatio-rvdd626 | María Dolores Rojo-Martín, Germán Peñalva, Carmen Pinto, Inmaculada Salcedo, Rocío Fernández-Urrusuno, José Cabeza, Juan de Dios Alcántara, Olaf Neth, Paloma Porras, Javier Bautista, José Garnacho-Montero, Rafael Sierra, Ángel Estella, Carmen Lupión, Elena Hevia, Arantxa Irastorza, José Luis Márquez, María Luisa Garcí... | TITLE: The PIRASOA programme: design, structure, organisation and indicators of a comprehensive regional Institutional Programme for the Prevention and Control of Healthcare-associated Infections and Antimicrobial Stewardship for hospitals and primary care settin
AUTHORS: María Dolores Rojo-Martín, Germán Peñalva, Car... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.d5m845 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This tutorial explains how to download entire libraries of data from the VIROME web application. <br /><br />Wommack, K. E., J. Bhavsar, S. W. Polson, J. Chen, M. Dumas, S. Srinivasiah, M. Furman, S. Jamindar, and D. J. Nasko. 2012. VIROME: a standard operating procedure for ana... | [] |
75,631 | Non-invasive Detection Method for Bonamia ostreae in Ostrea edulis using the Franklin qPCR machine | 4 | dx.doi.org/10.17504/protocols.io.q26g7y311gwz/v1 | https://www.protocols.click/view/non-invasive-detection-method-for-bonamia-ostreae-cm4pu8vn | Lavanya M Vythalingam, Tim Regan, Tim Bean | TITLE: Non-invasive Detection Method for Bonamia ostreae in Ostrea edulis using the Franklin qPCR machine
AUTHORS: Lavanya M Vythalingam, Tim Regan, Tim Bean
[DESCRIPTION]
This is a comprehensive protocol for using the Franklin qPCR (Biomeme Inc.) machine to detect the presence of Bonamia ostreae parasite in European ... | ["[Sample Collection] Separate collected animals and cover with seawater (approx. 1 L for every 200 g of live animal).", "[Sample Collection] Collect oysters and wash to remove any excess mud/sediment on their shells.", "[Sample Collection] Aerate buckets overnight for 960 min at ambient temperature on sampling locatio... |
28,110 | Preparation of Chemically Competent Cells | null | dx.doi.org/10.17504/protocols.io.7pnhmme | null | NUS iGEM | TITLE: Preparation of Chemically Competent Cells
AUTHORS: NUS iGEM
[STEPS]
?. Transfer of overnight culture into LB in a flask
1 ml
50 ml
?. Incubate at at until OD600 = 0.6
37 °C
Centrifuge: 225 33
?. Transfer culture to falcon tube
50 ml
?. Incubate culture on ice for
?. Centrifuge tube at , for
4 °C
Ce... | ["Transfer of overnight culture into LB in a flask\n1 ml\n50 ml", "Incubate at at until OD600 = 0.6\n37 °C\nCentrifuge: 225 33", "Transfer culture to falcon tube\n50 ml", "Incubate culture on ice for", "Centrifuge tube at , for\n4 °C\nCentrifuge: 5000 33", "Discard supernatant and resuspend pellet in of ... |
25,436 | Evaluation of sodium deoxycholate as solubilization buffer for oil palm proteomics analysis | null | dx.doi.org/10.17504/protocols.io.434gyqw | null | Benjamin Lau, Abrizah Othman | TITLE: Evaluation of sodium deoxycholate as solubilization buffer for oil palm proteomics analysis
AUTHORS: Benjamin Lau, Abrizah Othman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Protein solubility is a critical prerequisite to any proteomics analysis. Combination of urea/thiourea and 3-... | ["[Protein extraction from mesocarp]\n10 g of sliced mesocarps were ground and mixed well with 25 mL of cold acetone containing 10% trichloroacetic acid and 1 mM dithiothreitol on ice.", "[Protein extraction from mesocarp]\nThe slurry was then centrifuged at 13,000 g for 10 min at 4°C (RA-300 rotor, Kubota 7820, Kubota... |
13,913 | High-throughput assessment of changes in the Caenorhabditis elegans gut microbiome | 1 | dx.doi.org/10.17504/protocols.io.rtzd6p6 | https://www.protocols.io/view/high-throughput-assessment-of-changes-in-the-caeno-rtzd6p6 | Fan Zhang, Jessica Weckhorst, Adrien Assie, Anastasia Khodakova, Mario Loeza Cabrera, Daniela Vidal, Christopher Ayoub, Buck Samuel | TITLE: High-throughput assessment of changes in the Caenorhabditis elegans gut microbiome
AUTHORS: Fan Zhang, Jessica Weckhorst, Adrien Assie, Anastasia Khodakova, Mario Loeza Cabrera, Daniela Vidal, Christopher Ayoub, Buck Samuel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The gut microbi... | ["[High-throughput Colonization Assay]\nWash worms from bacterial lawns with M9 buffer + Triton X to a sterilized 2 ml 96-well deep plate.Use M9+ Triton X to wash worms from a 12-well plateUse M9+ Triton X to wash worms from a 24-well plate\n1000 µl\n600 µl\n{\"blocks\":[{\"data\":[],\"depth\":0,\"entityRanges\":[],\... |
100,316 | Efficacy and safety of statin therapy in kidney transplant recipients: a meta-analysis | 0 | dx.doi.org/10.17504/protocols.io.5qpvok3yzl4o/v1 | https://www.protocols.io/view/efficacy-and-safety-of-statin-therapy-in-kidney-tr-dd7429qw | Ioannis Bellos, Vassiliki Benetou | TITLE: Efficacy and safety of statin therapy in kidney transplant recipients: a meta-analysis
AUTHORS: Ioannis Bellos, Vassiliki Benetou
[DESCRIPTION]
The present systematic review and meta-analysis aims to evaluate the effiacy and safety of HMG CoA Reductase Inhibitor (statin) therapy among kidney transplant recipien... | ["Objective To determine the efficacy and safety of HMG CoA reductase inhibitor (statin) treatment in kidney transplant recipients.", "Eligibility criteria The population of the study will consist of kidney transplant recipients. The intervention of interest will be statin administration, given for primary or secondary... |
69,731 | 3' TagSeq Library Preparation Protocol | 4 | dx.doi.org/10.17504/protocols.io.q26g7yrr3gwz/v1 | https://www.protocols.io/view/3-39-tagseq-library-preparation-protocol-cgcbtssn | anni.wang | TITLE: 3' TagSeq Library Preparation Protocol
AUTHORS: anni.wang
[DESCRIPTION]
Purpose: This protocol is used to construct libraries directed at the 3' ends of mRna for gene expression profiling studies and provides an alternative to standard RNA-Seq.
[STEPS]
SECTION: RNA Fragmentation and RT Primer Annealing
1... | ["[RNA Fragmentation and RT Primer Annealing] RNA Fragmentation and RT Primer Annealing\n\n*Input material from customer should be AT LEAST 25ul of high quality DNAse-treated RNA in water ideally normalized to 50ng/ul. (acceptable range is 5ng/ul to 100ng/ul).\n\n\n1.1 Clean bench, pipets, ice bucket, and all other equ... |
null | null | null | dx.doi.org/10.17504/protocols.io.rdwd27e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Cellectis, a French biopharmaceutical company, recently published a paper in the Scientific Reports, a publication of the Nature Publishing Group, and reported on the details of the CubiCAR developed by the company. CubiCAR is a novel chimeric antigen receptor (CAR) that carr... | [] |
67,441 | Arduino | 1 | null | https://www.protocols.io/view/arduino-cd4rs8v6 | bibewih | TITLE: Arduino
AUTHORS: bibewih
[DESCRIPTION]
This guide shows how to assemble The electronic components of the Mock Coastal Environment. This build uses a prototyping breadboard and wires. It should be noted that soldering of components and wires would make a more permanent solution but is outside the scope of this... | ["Wire components to Arduino board as depicted in wiring diagrams.", "Setup breadboard by attaching a red wire from (+) to 5V on and black (-) wires to GND on Arduino board.", "Wire the waterproof thermometer sensor as shown in the diagram above. The red wire to (+) and the black wire to (-) on breadboard. A 4.7k Ohm ... |
null | null | null | dx.doi.org/10.17504/protocols.io.tqaemse | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Primary cortical neuronal-astrocyte cell culture preparation. Dissociation, preparation and plating of mice cortex neurons and glia cells on MEA.
[STEPS]
SECTION: Sample collection
?.
SECTION: Cell lysis
?.
SECTION: Cell preparation
?.
SECTION: Cell culture
?. | ["[Sample collection] {\"blocks\":[{\"key\":\"5do5l\",\"text\":\"Dissect cortices from pups and place them on ice.\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"6hjmk\",\"text\":\" \",\"type\":\"atomic\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[{\... |
null | null | null | dx.doi.org/10.17504/protocols.io.c8hzt5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Preparation of trace metal stock solutions for cyanobacteria trace metal mixture (CTMM)
[BEFORE_START]
Before culturing cyanobacteria, read the publication by L.R. Moore et. al. (2007) Limnol. Oceanogr. 5: 353-362.<br />Purchase the highest quality chemicals to avoid trace meta... | [] |
40,048 | FCMPASS Protocol Collection | 5 | dx.doi.org/10.17504/protocols.io.bjcqkivw | https://www.protocols.io/view/fcmpass-protocol-collection-bjcqkivw | Joshua Welsh, Jennifer Jones | TITLE: FCMPASS Protocol Collection
AUTHORS: Joshua Welsh, Jennifer Jones
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is collection contains the protocols required for each step in the fcmpass software pipeline for performing small particle calibration using the fcmpass software package.</di... | ["[Cataloguing Reference Beads]\n{\"blocks\":[{\"key\":\"fo96r\",\"text\":\"This protocol outlines the steps required to catalogue light scatter reference materials using the FCMPASS software. This is one of a number of protocols in the pipeline for performing small particle calibration using the fcmpass software packa... |
70,896 | Colony PCR | 4 | dx.doi.org/10.17504/protocols.io.bp2l69bnklqe/v1 | https://www.protocols.io/view/colony-pcr-chgqt3vw | An.Huang | TITLE: Colony PCR
AUTHORS: An.Huang
[DESCRIPTION]
Colony PCR using the whole organism of bacteria instead of purified DNA template. This simplifies PCR procedure. This protocol helps conduct a simple colony PCR procedure.
[STEPS]
SECTION: Colony PCR
7. Add 30 µL TE buffer to X+2 1.5ml microcentrifuge tubes each. Labe... | ["[Colony PCR] Add 30 µL TE buffer to X+2 1.5ml microcentrifuge tubes each. Label the tubes as \"1, 2, 3, ..., X, +, -\".", "[Colony PCR] Place the tubes in a heating block, heating at 100 °C for 5 min.", "[Colony PCR] Prepare Master Mix for colony PCR. The recipe for the Master Mix is as follows:", "[Colony PCR] Pipet... |
60,117 | NCBI submission protocol for SARS-CoV-2 wastewater data: SRA, BioSample, and BioProject | 1 | dx.doi.org/10.17504/protocols.io.ewov14w27vr2/v6 | https://www.protocols.io/view/ncbi-submission-protocol-for-sars-cov-2-wastewater-b6xvrfn6 | Ruth Timme, Maria Balkey | TITLE: NCBI submission protocol for SARS-CoV-2 wastewater data: SRA, BioSample, and BioProject
AUTHORS: Ruth Timme, Maria Balkey
[DESCRIPTION]
PURPOSE:
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in ... | ["["Ingredients" to have in place before starting your submissions] Set up a new NCBI submission environment for your lab\n1.1: Create an NCBI user account\n1.2: Set up an NCBI submission user group for your lab\n1.3: Bookmark the link to your Submission Portal\n1.4. Identify or establish new BioProjects (det... |
59,622 | Quantification of SARS-CoV-2 variant mutations (HV69-70, E484K/N501Y, del156-157/R158G, del143-145, and LPPA24S) in settled solids using digital RT-PCR | 1 | dx.doi.org/10.17504/protocols.io.14egnzrrzg5d/v4 | https://www.protocols.io/view/quantification-of-sars-cov-2-variant-mutations-hv6-b6gerbte | Bridgette Hughes, Bradley J. White, Marlene K. Wolfe, Alexandria B B Boehm | TITLE: Quantification of SARS-CoV-2 variant mutations (HV69-70, E484K/N501Y, del156-157/R158G, del143-145, and LPPA24S) in settled solids using digital RT-PCR
AUTHORS: Bridgette Hughes, Bradley J. White, Marlene K. Wolfe, Alexandria B B Boehm
[DESCRIPTION]
This process instruction describes the steps for quantitativ... | ["[Preparation] Retrieve all kit components from the One-Step RT-ddPCR advanced kit for probes from the -20 °C freezer and thaw the components on ice.", "[Preparation] Retrieve ddPCR positive control aliquots (50 copies per uL gRNA) from the -80 °C freezer and thaw on ice", "[Preparation] For re-running frozen plates o... |
27,766 | Q5 PCR DNA Amplificaiton (Protocol for Q5® High-Fidelity 2X Master Mix) | null | dx.doi.org/10.17504/protocols.io.7cwhixe | null | Alba Balletbó | TITLE: Q5 PCR DNA Amplificaiton (Protocol for Q5® High-Fidelity 2X Master Mix)
AUTHORS: Alba Balletbó
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for PCR with Q5® High-Fidelity 2X Master Mix</div></div>
[STEPS]
?. Gently mix the PCR reactions and transfer the tubes to a thermoc... | ["Gently mix the PCR reactions and transfer the tubes to a thermocycler. Thermocycling conditions for a routine PCR: ABC1StepTemperatureTime2Initial Denaturation98°C30 seconds325–35 Cycles98°C5–10 seconds4*50–72°C10–30 seconds572°C20–30 seconds/kb6Final Extension72°C2 minutes7Hold4–10°C \nABC1StepTemperatureTime2... |
null | null | null | dx.doi.org/10.17504/protocols.io.rprd5m6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>KIRA-ELISA is used for quantitave detection of phosphorylation level of tyrosine kinase receptors. The protocol could be used for many different receptors. In our work, this protocol has been successful for TrkB and FGFR1 receptors. The underlying principle of the assay is ca... | [] |
106,084 | Cytopathology Quantification in iPSCs with Harmony Software | 0 | dx.doi.org/10.17504/protocols.io.yxmvmebnng3p/v1 | https://www.protocols.io/view/cytopathology-quantification-in-ipscs-with-harmony-djuc4nsw | Yang Liu, Jessica Chedid, YuHong Fu, Glenda Halliday | TITLE: Cytopathology Quantification in iPSCs with Harmony Software
AUTHORS: Yang Liu, Jessica Chedid, YuHong Fu, Glenda Halliday
[DESCRIPTION]
The following protocol details microscopy analysis of cortical and midbrain dopaminergic differentiated iPSC's. Quantification of tau, phosphorylated tau, p62, alpha synuclein ... | ["[1. Detection and Quantification of Neurons] Cell nuclei were detected and counted using the “Find Nuclei” block in the DAPI channel.", "[1. Detection and Quantification of Neurons] Calculate Morphology Properties” block was used to assess the nucleus area, ratio, and roundness for nuclei screening.", "[2. P62 Pathol... |
null | null | null | dx.doi.org/10.17504/protocols.io.iiyccfw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
25,107 | Regular non-enzymatic splitting of human pluripotent stem cells | null | dx.doi.org/10.17504/protocols.io.4rtgv6n | null | Ralitsa RRM. Madsen | TITLE: Regular non-enzymatic splitting of human pluripotent stem cells
AUTHORS: Ralitsa RRM. Madsen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the non-enzymatic splitting of human pluripotent stem cells with ReLeSR (Stem Cell Technologies) and RevitaCell (Thermo Fisher ... | ["[Preparatory work]\nPretreatment of the cells with E8/F+R (see Guidelines for abbreviations)\nEnsure that the medium is fresh and has spent minimum time outside the fridge, thus limiting growth factor degradation. I typically make fresh aliquots from the stock medium in the fridge, thus keeping the stock at for mos... |
null | null | null | dx.doi.org/10.17504/protocols.io.cq8vzv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
OneTaq DNA Polymerase allows for greater amplification sensitivity across a wide variety of amplicons regardless of GC content.
[GUIDELINES]
<strong>OVERVIEW<br /><br />PCR </strong><br /><br />The Polymerase Chain Reaction (PCR) is a powerful and sensitive tech... | [] |
20,163 | U Mass - Glucose Tolerance Test with insulin secretion | null | dx.doi.org/10.17504/protocols.io.xxbfpin | null | Jason Kim | TITLE: U Mass - Glucose Tolerance Test with insulin secretion
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">Glucose tolerance test with insulin secretion measures systemic clearance of glucose and s... | ["Mice may be fasted overnight (~15 hours) or for 5 hours prior to the start of experiment.", "Collect plasma sample (20 l) before the start of experiment (basal-0 min) to measure basal glucose and insulin levels.", "Administer intraperitoneal injection of 20% dextrose (1 or 2 g/kg body weight) using an insulin syring... |
63,854 | Crasher Keto Gummies Rewiew | 3 | dx.doi.org/10.17504/protocols.io.261genjmjg47/v1 | https://www.protocols.io/view/crasher-keto-gummies-rewiew-caknscve | DtieSmuh | TITLE: Crasher Keto Gummies Rewiew
AUTHORS: DtieSmuh
[DESCRIPTION]
crasher keto gummies help you increase your energy, and strength, to burn fat fast & get a slim figure *buy now on official site*
[STEPS] | [] |
44,591 | Fluorospot Assay | 4 | dx.doi.org/10.17504/protocols.io.bpspmndn | https://www.protocols.io/view/fluorospot-assay-bpspmndn | cecilia , Alessandro Sette | TITLE: Fluorospot Assay
AUTHORS: cecilia , Alessandro Sette
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details the steps required to collect and assay mouse anti-human cells using fluorospot assay.</div></div>
[STEPS]
?. [Day 1]
Coat plates with antibodies.
?. [Day 1]
Leave plate... | ["[Day 1]\nCoat plates with antibodies.", "[Day 1]\nLeave plates at .\n4 °C", "[Day 2]\nBlock plates:", "[Day 2]\nDiscard coating antibody and tap on paper.", "[Day 2]\nWash with / well 3x.\n[PBS]", "[Day 2]\nAdd .\n[culture media]", "[Day 2]\nIncubate at for .\n37 °C", "[Day 2]\nHarvest cells from culture wells (14... |
25,601 | 07 Extraction of Plasmid | null | dx.doi.org/10.17504/protocols.io.489gzz6 | null | TJUSLS China | TITLE: 07 Extraction of Plasmid
AUTHORS: TJUSLS China
[STEPS]
?. Collect the E. coli solution into the EP tube. Centrifuge at 12,000 rpm in a rotor for 1 minute. Remove the clear supernatant liquid.
?. Add 250μL P1 (RNase A added, kept at 4 °C)to the EP tube to suspend bacterial precipitation.
4 °C
250 µl
?. Add 250μL... | ["Collect the E. coli solution into the EP tube. Centrifuge at 12,000 rpm in a rotor for 1 minute. Remove the clear supernatant liquid.", "Add 250μL P1 (RNase A added, kept at 4 °C)to the EP tube to suspend bacterial precipitation.\n4 °C\n250 µl", "Add 250μL P2 to the EP tube, shake slightly up and down 6-8 times to ly... |
87,056 | Fully Automated Senescence Test (FAST) | 4 | null | https://www.protocols.io/view/fully-automated-senescence-test-fast-cy9qxz5w | Francesco Neri, Selma N. Takajjart, Chad A. Lerner, Pierre-Yves Desprez, Birgit Schilling, Judith Campisi, Akos A. Gerencser | TITLE: Fully Automated Senescence Test (FAST)
AUTHORS: Francesco Neri, Selma N. Takajjart, Chad A. Lerner, Pierre-Yves Desprez, Birgit Schilling, Judith Campisi, Akos A. Gerencser
[DESCRIPTION]
Cellular senescence is a major driver of aging and age-related diseases. Quantification of senescent cells remains challengin... | ["[Protocol Overview] This protocol is for measuring senescence-associated biomarkers by using the FAST workflow.\n\nFAST paper: INSERT LINK\n\nNote that this protocol does NOT include senescence induction methods. To induce senescence in your cell culture model using established methods, some of the most common protoc... |
74,115 | UPitt TriState SenNet TMC Single Cell RNA Seq (10X Genomics) Library Preparation and Sequencing 5' with Cell Hashings | 4 | dx.doi.org/10.17504/protocols.io.n2bvj89exgk5/v1 | https://www.protocols.io/view/upitt-tristate-sennet-tmc-single-cell-rna-seq-10x-ckmbuu2n | Tracy Tabib, Oliver Eickelberg, koenigshoffm, Robert Lafyatis | TITLE: UPitt TriState SenNet TMC Single Cell RNA Seq (10X Genomics) Library Preparation and Sequencing 5' with Cell Hashings
AUTHORS: Tracy Tabib, Oliver Eickelberg, koenigshoffm, Robert Lafyatis
[DESCRIPTION]
This protocol is for the generation of 5' cDNA libraries from individual cells via droplet generation uti... | ["[GEM Generation & Barcoding] Preparation of Single Cell Master Mix:\n a. Prepare Master Mix on ice. Pipette mix 15x and centrifuge briefly.\n\n\n \n\n\n b. Add 36.3 µl Master Mix into each tube of a PCR 8-tube strip on ice.", "[GEM Generation & Barcoding] Load Chromium Next GEM Chip K:\n a. Assemble ... |
40,886 | Bulk EV staining with CFSE protein binding dye | 1 | dx.doi.org/10.17504/protocols.io.bj6wkrfe | https://www.protocols.io/view/bulk-ev-staining-with-cfse-protein-binding-dye-bj6wkrfe | Aizea Morales-Kastresana, Joshua Welsh, Jennifer Jones | TITLE: Bulk EV staining with CFSE protein binding dye
AUTHORS: Aizea Morales-Kastresana, Joshua Welsh, Jennifer Jones
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Carboxyfluorescein Diacetate Succynidimil Esther (CFDA-SE) is a small molecule that has been used extensively for labeling and tracki... | ["Prepare 15 µL of DPBS containing between ~1x108 to ~2.5 x 109 DC2.4-derived EVs in a 1.7 ml microfuge tube.\nNote: This number of EVs correspond to ~ 0.1-2.5 µL of EV stock, if prepared as described previously(Morales-Kastresana et al., 2017), starting with 60 ml of supernatant from cells cultured for 48h in EV deple... |
null | null | null | dx.doi.org/10.17504/protocols.io.c5xy7m | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<span style="text-decoration: underline;"><strong>References:</strong></span><br /> <span style="font-family: Arial,Helvetica,sans-serif;">Becton Dickinson Immunocytometry Systems Source Book (1989) 2.10 <br /></span> <span style="font-family: Arial,Helvetica,sans-serif;">Lanier... | [] |
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