id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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70,867 | Electrophysiology | 1 | dx.doi.org/10.17504/protocols.io.4r3l274mjg1y/v1 | https://www.protocols.io/view/electrophysiology-chftt3nn | gurvir.virdi | TITLE: Electrophysiology
AUTHORS: gurvir.virdi
[DESCRIPTION]
Electrophysiology of iPSC-derived mDA neurons
[STEPS]
SECTION: Electrophysiology
1. Visualized patch-clamp recordings from cell cultures were performed using an infrared differential interference contrast imaging system and a Multipatch 700B amplifier contr... | ["[Electrophysiology] Visualized patch-clamp recordings from cell cultures were performed using an infrared differential interference contrast imaging system and a Multipatch 700B amplifier controlled by pClamp 10.2 software package (Molecular Devices, USA).", "[Electrophysiology] For the recordings, a neuronal culture... |
22,712 | Soil collection and preparation for pot experiments | null | dx.doi.org/10.17504/protocols.io.2eygbfw | null | Matema LE. Imakumbili | TITLE: Soil collection and preparation for pot experiments
AUTHORS: Matema LE. Imakumbili
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to collect and prepare soil that will be used as a potting medium (potting soil) in a pot experiment.</div></div>
[STEPS]
?. Determin... | ["Determining the amount of soil needed for the pot experimentMake sure you determine the amount of soil you shall need before setting out to collect the soil from the field. You can make the calculation by multiplying the amount of soil to be placed in each pot by the number of pots you will use to set-up the entire e... |
52,128 | Oxidative Stress Cell Model | 4 | dx.doi.org/10.17504/protocols.io.bw58pg9w | https://www.protocols.io/view/oxidative-stress-cell-model-bw58pg9w | Veerle Baekelandt | TITLE: Oxidative Stress Cell Model
AUTHORS: Veerle Baekelandt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = ":UNDERLINE;">Oxidative Stress Cell Model preparation for intact cell cross-linking</span></div></div>
[STEPS]
?. seed cells for in a 10 cm dish at a density of 6 x 106 cell... | ["seed cells for in a 10 cm dish at a density of 6 x 106 cells per plate", "treat cells for with 5 mM freshly prepared FeCl2 and 100 µM H2O2 in DMEM-complete (filtered trough a .45µM filter)", "wash cells with 1X PBS and scrape cells for immunoblotting or intact cell cross linking"] |
93,724 | Protocol 1 | 5 | dx.doi.org/10.17504/protocols.io.ewov1qbkygr2/v2 | https://www.protocols.io/view/protocol-1-c7r4zm8w | Mitt Coats | TITLE: Protocol 1
AUTHORS: Mitt Coats
[DESCRIPTION]
A short abstract
[STEPS]
3.
SECTION: S2
4.
SECTION: S2
5.
SECTION: S2
6.
SECTION: S0
1.
SECTION: S1
2.
SECTION: S2
7. Done
| ["[S2]", "[S2]", "[S2]", "[S0]", "[S1]", "[S2] Done"] |
105,314 | USDA LTAR Common Experiment measurement: Total suspended solids (TSS) | 1 | dx.doi.org/10.17504/protocols.io.261ge5pjog47/v2 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-total-susp-di4a4gse | Brent Dalzell, Oliva Pisani | TITLE: USDA LTAR Common Experiment measurement: Total suspended solids (TSS)
AUTHORS: Brent Dalzell, Oliva Pisani
[DESCRIPTION]
Total suspended solids (TSS) refers to all the material from a well-mixed sample retained by a filter media. In the case of environmental water samples, TSS is commonly used to measure sedime... | ["[Sample collection] At a minimum, collect 1 mg of TSS residue. At a maximum, TSS should not impede the flow rate of the filter media (slow filtration is more time-consuming but does not impact results).", "[Sample collection] Return samples to the laboratory on ice and filter them on collection day if possible.", "[S... |
44,055 | Adherence to Antiretrovial Therapy in Patients with HIV / AIDS: Combination Therapy X Conventional Therapy | 1 | dx.doi.org/10.17504/protocols.io.bn9xmh7n | https://www.protocols.io/view/adherence-to-antiretrovial-therapy-in-patients-wit-bn9xmh7n | Poliana Moreira de Medeiros Carvalho, rmsneto_ | TITLE: Adherence to Antiretrovial Therapy in Patients with HIV / AIDS: Combination Therapy X Conventional Therapy
AUTHORS: Poliana Moreira de Medeiros Carvalho, rmsneto_
[STEPS]
?. Adherence to Antiretrovial Therapy in Patients with HIV / AIDS: Combination Therapy X Conventional Therapy
?. Poliana Moreira de Medeiros... | ["Adherence to Antiretrovial Therapy in Patients with HIV / AIDS: Combination Therapy X Conventional Therapy", "Poliana Moreira de Medeiros CarvalhoPharmaceuticals, PhD by the Postgraduate Program in Health Sciences, ABC Medical School (FMABC), Santo André, SP, Brazil. https://orcid.org/0000-0002-2470-1923Raimundo Mont... |
27,983 | Yeast competent cells | null | dx.doi.org/10.17504/protocols.io.7jphkmn | null | Marijn Ceelen | TITLE: Yeast competent cells
AUTHORS: Marijn Ceelen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol to make yeast competent cells, which can be then stored at 4 ºC for up to a week. </div></div>
[STEPS]
?. Pick a yeast colony from a plate and place it inside 10 mL YPD medium.
?. ... | ["Pick a yeast colony from a plate and place it inside 10 mL YPD medium.", "Incubate the cells overnight at 30 ºC and 180 rpm.", "The next day, measure the OD600 of the liquid culture.", "Inoculate a flask with 50 mL YPD medium with enough volume of the overnight culture to reach an OD600 of 0.4.", "Incubate the cells ... |
80,387 | Wound Care Protocol (step-by-step protocol) | 1 | dx.doi.org/10.17504/protocols.io.n2bvj8wqpgk5/v1 | https://www.protocols.io/view/wound-care-protocol-step-by-step-protocol-csrbwd2n | rodrigo.raimundo | TITLE: Wound Care Protocol (step-by-step protocol)
AUTHORS: rodrigo.raimundo
[DESCRIPTION]
Chronic lower limb ulcers (CLLU) are those injuries that persist for more than six weeks despite adequate care. They are relatively common; it is estimated that 10/1,000 people will develop CLLU in their lifetime. Diabetic ulcer... | ["[Wound preparation] Cleaning and antisepsis of chronic wounds were performed with an aqueous solution of chlorhexidine and 0.9% saline before applying the blood concentrates", "[Blood Concentrate Preparation (PRO-PRF)] Blood samples were obtained by venipuncture, the patient remained comfortably seated, the upper lim... |
46,311 | NAO staining | 4 | null | https://www.protocols.io/view/nao-staining-brgfm3tn | Elizabeth Fozo | TITLE: NAO staining
AUTHORS: Elizabeth Fozo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">NAO staining protocol - Harp - 2014</div></div>
[STEPS]
?. [Steps]
Incubate Enterococcus faecalis at 37oC overnight in 10mL of Brain Heart Infusion (BHI) broth.
?. [Steps]
The next morning check OD600 of ove... | ["[Steps]\nIncubate Enterococcus faecalis at 37oC overnight in 10mL of Brain Heart Infusion (BHI) broth.", "[Steps]\nThe next morning check OD600 of overnight culture. Should be around ~1.5.", "[Steps]\nDiluted overnight culture to 0.01 using fresh 10mL BHI broth.", "[Steps]\nAdd supplements as necessary.", "[Steps]\nI... |
12,285 | In situ PCR using leaf epidermal peels | null | dx.doi.org/10.17504/protocols.io.p85dry6 | null | Diep R. Ganguly, Asmini Athman | TITLE: In situ PCR using leaf epidermal peels
AUTHORS: Diep R. Ganguly, Asmini Athman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Method for visualizing cell-localized gene expression patterns.</div><div class = "text-block">Adapted from: </div><div class = "text-block">Athman, A., Tanz, S.K., C... | ["[Slide preparation (day before)]\nThe day before: prepare slides by placing frame seals on glass slide and apply pressure to borders to seal. These can be heated in thermal cycler at 90 °C for 10 minutes. Cut off excess seal to allow slide to sit appropriately in PCR machine.", "[Sample preparation and fixation]\nPre... |
61,022 | Reconstitution of LRRK2 membrane recruitment onto planar lipid bilayers | 4 | dx.doi.org/10.17504/protocols.io.x54v9y7qzg3e/v1 | https://www.protocols.io/view/reconstitution-of-lrrk2-membrane-recruitment-onto-b7t6rnre | Ayan Adhikari, Edmundo Vides, Suzanne Pfeffer | TITLE: Reconstitution of LRRK2 membrane recruitment onto planar lipid bilayers
AUTHORS: Ayan Adhikari, Edmundo Vides, Suzanne Pfeffer
[DESCRIPTION]
Supported lipid bilayers have emerged as an ideal model system to study the interaction of proteins with cellular membranes. We describe here a method to monitor the rec... | ["[Method for cleaning glass coverslips] Clean Lab-TeKII 8 chamber, No. 1.5 borosilicate coverglasses (Fisher) using Piranha solution by 30 min incubation followed by extensive washing in Milli-Q water.", "[Method for cleaning glass coverslips] Before the experiments, dry the chambers and clean further using a Harrick ... |
37,662 | Blasticidin titration of cancer cell lines | 1 | dx.doi.org/10.17504/protocols.io.bgz6jx9e | https://www.protocols.io/view/blasticidin-titration-of-cancer-cell-lines-bgz6jx9e | Emily Souster, Verity Goodwin, Adam Jackson, Charlotte Beaver, Rizwan Ansari, Fiona Behan, Mathew Garnett | TITLE: Blasticidin titration of cancer cell lines
AUTHORS: Emily Souster, Verity Goodwin, Adam Jackson, Charlotte Beaver, Rizwan Ansari, Fiona Behan, Mathew Garnett
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to identify the optimum blasticidin concentration for the selecti... | ["[Day 1: Titration plate set up]\nDetach, collect and count cells by following Steps 1-8 of the protocol: Passaging adherent cancer cell lines.", "[Day 1: Titration plate set up]\nResuspend 5x105 cells in of culture media, at a concentration of 1x105 cells/ml.\n5 mL", "[Day 1: Titration plate set up]\nUsing a 10mg/ml... |
21,273 | Vandy – IP glucose tolerance test | null | dx.doi.org/10.17504/protocols.io.yzzfx76 | null | Louise Lantier | TITLE: Vandy – IP glucose tolerance test
AUTHORS: Louise Lantier
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">The glucose tolerance test measures the clearance of an intraperitoneally injected glucose load from the ... | ["Weigh the mice. For mice of differing fat mass, we recommend dosing the bolus of glucose on lean mass. This requires body composition.", "Fast mice for 5 hours by transferring mice to individual cages or containers.", "Prepare an experiment record sheet, sticks for glucose measurement and syringe for IP injection of ... |
73,385 | Flow cytometric measurement of STAT5 phosphorylation to assess the activity of common γ chain cytokines in T cells | 1 | dx.doi.org/10.17504/protocols.io.dm6gpjzd1gzp/v1 | https://www.protocols.io/view/flow-cytometric-measurement-of-stat5-phosphorylati-cjwhupb6 | Zaki Molvi | TITLE: Flow cytometric measurement of STAT5 phosphorylation to assess the activity of common γ chain cytokines in T cells
AUTHORS: Zaki Molvi
[DESCRIPTION]
Common γ chain cytokines such as IL2, IL15, and IL7 activate the JAK3/STAT5 signaling cascade, resulting in rapid phosphorylation of STAT5. The assay described he... | ["[Introduction] Common γ chain cytokines such as IL2, IL15, and IL7 activate the JAK3/STAT5 signaling cascade, resulting in rapid phosphorylation of STAT5. The assay described herein, based on the original protocol by Krutzik & Nolan, details the use of flow cytometry to quantify the levels of phospho-STAT5 (pSTAT5) a... |
62,429 | Bioinformatic workflow for NGS data control | 5 | dx.doi.org/10.17504/protocols.io.8epv59bnjg1b/v1 | https://www.protocols.io/view/bioinformatic-workflow-for-ngs-data-control-b875rzq6 | Khalid El Moussaoui | TITLE: Bioinformatic workflow for NGS data control
AUTHORS: Khalid El Moussaoui
[DESCRIPTION]
Workflow for data integrity and quality control of high throughput sequencing on Illumina NovaSeq6000. The analyses are performed on macOS Monterey 12.3.1 running on an ARM-architected Apple Silicon processor. This workflow ... | ["[Activation of the environment] Open a terminal window.", "[Activation of the environment] Activate the previously created QC_env environment by typing the following command in the terminal :", "[Data integrity check] Considering that the .gz archive downloaded from the GIGA servers has been unzipped under ~/fastq_fi... |
75,192 | Reynolds' Lead Citrate Stain | 6 | null | https://www.protocols.io/view/reynolds-39-lead-citrate-stain-cmnyu5fw | Jens Berndtsson | TITLE: Reynolds' Lead Citrate Stain
AUTHORS: Jens Berndtsson
[DESCRIPTION]
Reynolds Lead Citrate Stain for electron microscopy is a standard stain developed by Edward S. Reynolds and consists of a mixture of 80 mM Lead(II) nitrate and 120 mM Sodium citrate. Sodium hydroxide is added to make the stain basic to pH 1... | ["[50 ml Lead citrate for Array tomography] Weigh out and dissolve Lead (II) nitrate and tri-Sodium citrate dihydrate:\nWeigh out 1.32 g of in a 50 ml volumetric flask in which you have added a small magnetic stirrer.\nDissolve the Lead(II) nitrate by adding 30 mL of and turning on the stirrer.\nWeigh out 1.76 g of... |
93,027 | Culture of human epithelial cells (skin, cornea, thymus) on 3T3J2 feeder layer cells | 4 | dx.doi.org/10.17504/protocols.io.14egn31ezl5d/v1 | https://www.protocols.io/view/culture-of-human-epithelial-cells-skin-cornea-thym-c64bzgsn | Howard Green | TITLE: Culture of human epithelial cells (skin, cornea, thymus) on 3T3J2 feeder layer cells
AUTHORS: Howard Green
[DESCRIPTION]
This document is of vital importance for scientists attempting the culture of human
keratinocytes derived from skin, cornea, thymus, esophagus etc. Please strictly adhere
to the guidelines sh... | ["[Key steps for 3T3J2 feeders growth (Howard Green lab)] Obtain DMEM", "[Key steps for 3T3J2 feeders growth (Howard Green lab)] Add 8% of bovine serum (DO NOT use fetal calf serum! key point)", "[Key steps for 3T3J2 feeders growth (Howard Green lab)] Feed every 2 or 3 days", "[Key steps for 3T3J2 feeders growth (Howar... |
42,110 | Staining Sequenza | 4 | dx.doi.org/10.17504/protocols.io.bmc6k2ze | https://www.protocols.io/view/staining-sequenza-bmc6k2ze | Marc Bosse, Sean Bendall, Mike Angelo | TITLE: Staining Sequenza
AUTHORS: Marc Bosse, Sean Bendall, Mike Angelo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the use of sequenza staining device. The sequenza can be use for MIBI or IHC staining methods.</div></div>
[STEPS]
?. [Sequenza assembly]
Fill a disposable... | ["[Sequenza assembly]\nFill a disposable Pipetting Reservoir with of 1x PBS wash buffer\n20 mL", "[Sequenza assembly]\nPlace sample slide on a Sequenza cover plate aligning the bottom slide with the notches on the cover plate (see picture below panel A)", "[Sequenza assembly]\nFill by capillarity the space between the... |
60,416 | Stranded Transcript Count Table Generation from Long Reads | 1 | dx.doi.org/10.17504/protocols.io.5qpvonn2bl4o/v13 | https://www.protocols.io/view/stranded-transcript-count-table-generation-from-lo-b688rhzw | David A Eccles | TITLE: Stranded Transcript Count Table Generation from Long Reads
AUTHORS: David A Eccles
[DESCRIPTION]
This protocol is for comparing different samples at the transcript level, using long reads that are mapped to transcripts.
Input(s): demultiplexed and oriented fastq files (see protocol Preparing Reads for Strande... | ["[Demultiplex Reads] Demultiplex and orient reads as per the protocol Preparing Reads for Stranded Mapping. It is expected that these demultiplexed reads will be split up in the current directory, and coupled with a 'barcode_counts.txt' file. If that's the case, the following should work:\n \nExample expected output:\... |
26,144 | A Leading Service Provider in Phage Display & Antibody Library | null | dx.doi.org/10.17504/protocols.io.5r8g59w | null | Echo Han | TITLE: A Leading Service Provider in Phage Display & Antibody Library
AUTHORS: Echo Han
[STEPS]
?. About the CompanyFounded by scientists who are dedicated to the conquering of cancer, Creative Biolabs believes to build up a custom-service-centered business model that is crucial for optimizing the drug development pro... | ["About the CompanyFounded by scientists who are dedicated to the conquering of cancer, Creative Biolabs believes to build up a custom-service-centered business model that is crucial for optimizing the drug development process, leveraging accessible resources, and forming a team of various background to conduct drug di... |
78,442 | Leaf Protein Extraction for Immunoblot (Soybean, Cowpea, Tobacco) | 4 | null | https://www.protocols.io/view/leaf-protein-extraction-for-immunoblot-soybean-cow-cquivwue | Steven J Burgess | TITLE: Leaf Protein Extraction for Immunoblot (Soybean, Cowpea, Tobacco)
AUTHORS: Steven J Burgess
[DESCRIPTION]
This protein extraction protocol was developed for analysis of protein abundance in leaf tissue by immunoblot. It was optimized for dicot species including Glycine max.
Quantities - 450 µL protein extracti... | ["[Buffer Preparation] Incubate 4x PEB (if stored at 4 oC) at 50 °C for 20 min to re-suspended precipitated SDS in buffer.", "[Buffer Preparation] Make 500 µL 1x PEB per sample by diluting 4x stock with dH2O.", "[Dissolve ground powder] Add 450 µL 1x PEB to ground powder (3x 1cm leaf disk).", "[Dissolve ground powder] ... |
81,825 | NCEM Drop - Cell Pellet Dounce Homogenisation (TM-014) | 4 | dx.doi.org/10.17504/protocols.io.bp2l69j4dlqe/v2 | https://www.protocols.io/view/ncem-drop-cell-pellet-dounce-homogenisation-tm-014-ct59wq96 | sandra.crameri | TITLE: NCEM Drop - Cell Pellet Dounce Homogenisation (TM-014)
AUTHORS: sandra.crameri
[DESCRIPTION]
cell pellet dounce homogenisation
[STEPS]
SECTION: HEADER
1. SAN:
SPEC No:
OPERATOR:
SECTION: Dounce Homogenisation
2. Remove cells from the flasks by scraping with a disposable plastic scraper and poor into... | ["[HEADER] SAN:\n\n\n\n\nSPEC No:\n\n\n\n\nOPERATOR:", "[Dounce Homogenisation] Remove cells from the flasks by scraping with a disposable plastic scraper and poor into a 10 mL centrifuge tube.", "[Dounce Homogenisation] Pellet the cells in a bench top centrifuge at 2000 rpm 2 min , remove supernatant.", "[Dounce Homog... |
null | null | null | dx.doi.org/10.17504/protocols.io.jxccpiw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is from:<br /><br />Satinsky, Brandon M., et al. "<a href="http://www.sciencedirect.com/science/article/pii/B9780124078635000125" target="_blank">Use of internal standards for quantitative metatranscriptome and metagenome analysis.</a>" Methods in enzymology 531 (2... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.efzbbp6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol describes how to check the quality of modified QCM-D sensors and how to study protein interactions using QCM-D sensing.
[BEFORE_START]
All QCM-D measurements should be performed with an E4 Q-Sense instrument. All protein interaction experiments should be performed... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.c7cziv | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
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95,860 | PONE-D-23-30545 Stata codes | 1 | dx.doi.org/10.17504/protocols.io.yxmvm38pnl3p/v1 | https://www.protocols.io/view/pone-d-23-30545-stata-codes-c9uuz6ww | Fengming Chen, Hayato Nakanishi, Yoichi Sekizawa, Sae Ochi, Mirai So | TITLE: PONE-D-23-30545 Stata codes
AUTHORS: Fengming Chen, Hayato Nakanishi, Yoichi Sekizawa, Sae Ochi, Mirai So
[DESCRIPTION]
Fig1. RD plot of the probability of receiving the vaccine twice.
Table 2. Estimation Results of the Regression Discontinuity Design on Outcomes.
S3 Table. Estimation Result of the Effect of El... | [] |
45,678 | Islet Embedding for Histology | 4 | dx.doi.org/10.17504/protocols.io.bqunmwve | https://www.protocols.io/view/islet-embedding-for-histology-bqunmwve | IIDP-HIPP | TITLE: Islet Embedding for Histology
AUTHORS: IIDP-HIPP
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;font-style:italic;">This Standard Operating Procedure (SOP) is based on the Vanderbilt Human Islet Phenotyping Program (HIPP) Immobilization of Islets in 3-D Gel f... | ["[Procedures]\nIslet Immobilization and Fixation", "[Procedures]\nPrepare Collagen I working solution ( ) by combining Collagen I stock, sterile water, HEPES, NaHCO3, 5X DMEM.\n1 mL\n375 µl\n355 µl\n20 µl\n50 µl\n200 µl", "[Procedures]\nUsing a P-1000 pipette and wide-bore tips, transfer an aliquot of islet suspe... |
50,947 | Coral Resilience Lab StepOnePlus™ qPCR protocol for Cladocopium and Durusdinium Symbiodiniaceae detection | 1 | dx.doi.org/10.17504/protocols.io.bvzbn72n | https://www.protocols.io/view/coral-resilience-lab-steponeplus-qpcr-protocol-for-bvzbn72n | Mariana Rocha De Souza, Shayle Matsuda, Jenna Dilworth, Crawford Drury, Ross Cunning | TITLE: Coral Resilience Lab StepOnePlus™ qPCR protocol for Cladocopium and Durusdinium Symbiodiniaceae detection
AUTHORS: Mariana Rocha De Souza, Shayle Matsuda, Jenna Dilworth, Crawford Drury, Ross Cunning
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The purpose of this protocol is to ... | ["[Mastermix preparation]\nPrimer and Probe DilutionsPrimers usually come in lyophilized form. We recommend diluting them to a 100µM stock, then diluting the stock to get the desired concentration for the reactions. For the reacions, Forward primers should be 1μM and Reverse primers shold be 1.5 μM. Probes should be di... |
50,675 | Bioluminescence-based 24 well plate assay for screening fungi for activity against Mycobacterium abscessus | 1 | dx.doi.org/10.17504/protocols.io.bvqtn5wn | https://www.protocols.io/view/bioluminescence-based-24-well-plate-assay-for-scre-bvqtn5wn | Siouxsie Wiles, Alex Grey | TITLE: Bioluminescence-based 24 well plate assay for screening fungi for activity against Mycobacterium abscessus
AUTHORS: Siouxsie Wiles, Alex Grey
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"><span>There is a real and urgent need for new antibiot... | ["[Initial culturing of fungi]\nSub-culture your fungus of choice onto a Potato Dextrose Agar (PDA) plate from either an existing agar culture or from frozen stocks.", "[Initial culturing of fungi]\nSeal the plate with parafilm and store it in a plastic box at room temperature. Allow the culture to reach at least 50% g... |
52,194 | Protocol 2 | 1 | dx.doi.org/10.17504/protocols.io.bw8aphse | https://www.protocols.io/view/protocol-2-bw8aphse | Keira Smith | TITLE: Protocol 2
AUTHORS: Keira Smith
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Text of the description</div></div>
[STEPS]
?. | [] |
79,544 | Midbrain-like Organoids generation from hiPSCs | 4 | dx.doi.org/10.17504/protocols.io.kqdg39d5qg25/v1 | https://www.protocols.io/view/midbrain-like-organoids-generation-from-hipscs-crwyv7fw | Hariam Raji, michela.deleidi | TITLE: Midbrain-like Organoids generation from hiPSCs
AUTHORS: Hariam Raji, michela.deleidi
[DESCRIPTION]
In this protocol we describe the differentiation of human induced pluripotent stem cells (hiPSCs) into human midbrain-like organoids (hMLOs). This protocol has been developed based from several published protocols... | ["[Day 0] Dissociate iPSC colonies to single cells with Accutase for 7 min at 37 °C.", "[Day 0] Re-suspend cells in day0 medium and plate 8.000 cells/well in 96-Wells Plate U-round-Bottom Low Attachment.", "[Day 4] Carefully exchange the medium, without touching the EBs.", "[Day 7: Matrigel embedding] Dilute Matrigel i... |
27,729 | Bachelor/Masters thesis protocol | null | dx.doi.org/10.17504/protocols.io.7brhim6 | null | Eliza Harris | TITLE: Bachelor/Masters thesis protocol
AUTHORS: Eliza Harris
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><h1><span style = "font-weight:bold;">Protocol</span><span>: Bachelor and Master thesis students</span></h1></div></div>
[STEPS] | [] |
51,553 | Image processing to generate first-level product | 5 | dx.doi.org/10.17504/protocols.io.8epv5z5b6v1b/v1 | https://www.protocols.io/view/image-processing-to-generate-first-level-product-bwj9pcr6 | Wei-Ping Chan | TITLE: Image processing to generate first-level product
AUTHORS: Wei-Ping Chan
[DESCRIPTION]
Details could be found in the Supplementary Information of "A high-throughput multispectral imaging system for museum specimens"
[STEPS]
SECTION: Img Synchronization
2. Synchronizing images from the cloud drive. A cron job la... | ["[Img Synchronization] Synchronizing images from the cloud drive. A cron job launching this command can also be established to monitor the cloud drive regularly so the newly uploaded images will be synched to the target directory automatically. Note that if the token for the cloud drive is password-protected, the inte... |
45,997 | BEAST v1.X tutorial, in a case of four cicada genera | 4 | dx.doi.org/10.17504/protocols.io.bq6mmzc6 | https://www.protocols.io/view/beast-v1-x-tutorial-in-a-case-of-four-cicada-gener-bq6mmzc6 | Soichi Osozawa | TITLE: BEAST v1.X tutorial, in a case of four cicada genera
AUTHORS: Soichi Osozawa
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify">A Bayesian inference (BI) tree is constructed using the software BEAST v.1X (Bayesian Evolutionary Analysis Samplin... | ["General flow\n\t\t\t\t\t\t\t .justify:after {\n\t\t\t\t\t\t\t content: \"\";\n\t\t\t\t\t\t\t display:inline-block;\n\t\t\t\t\t\t\t width: 100%;\n\t\t\t\t\t\t\t }\n\t\t\t\t\t\t\tA Bayesian inference (BI) tree is constructed using the software BEAST v.1X (Bayesian Evolutionary Analysis Sampling Trees; Suchar... |
103,938 | BAF_Protocol_015_ 3-NPH derivatization of short chain fatty acid | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1oz7lmk/v1 | https://www.protocols.io/view/baf-protocol-015-3-nph-derivatization-of-short-cha-dhra352e | Nicholas Sherman | TITLE: BAF_Protocol_015_ 3-NPH derivatization of short chain fatty acid
AUTHORS: Nicholas Sherman
[DESCRIPTION]
Short Chain Fatty Acid derivitization before MS analysis.
[STEPS]
SECTION: Samples
1. Refer to BAF_Protocol_008 Metabolomics: Soluble Metabolite Extraction for soluble metabolite extraction of a sample of i... | ["[Samples] Refer to BAF_Protocol_008 Metabolomics: Soluble Metabolite Extraction for soluble metabolite extraction of a sample of interest and dry the extracted metabolites.", "[Standards and standard curve] Prepare a stock solution of each standard at 10 mg/mL in 75% MeOH. Use the stock solution to generate a 1000 ug... |
71,283 | Movie_Timepoint_CopytoClipboard_Tool | 5 | dx.doi.org/10.17504/protocols.io.n92ldp9e9l5b/v1 | https://www.protocols.io/view/movie-timepoint-copytoclipboard-tool-chutt6wn | Condon ND | TITLE: Movie_Timepoint_CopytoClipboard_Tool
AUTHORS: Condon ND
[DESCRIPTION]
This protocol describes how to use the Movie-Timepoint_CopytoClipboard_Tool.ijm program made by Nicholas Condon at the Institute for Molecular Bioscience's Microscopy core facility at The University of Queensland, Brisbane, Australia.
This ... | ["[Running the Script] Launch FIJI", "[Running the Script] Open your time-series dataset into FIJI", "[Running the Script] Open the Movie Timepoint CopytoClipboard Tool script\n[Plugins > Macros > Run...", "[Running the Script] Read the instructions & acknowledgement window", "[Running the Script] Ensure a time-series ... |
null | null | null | dx.doi.org/10.17504/protocols.io.iu2ceye | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is following RNA-SIP experiments and RNA extraction to fractionate the RNA according to density. The protocol is based on Leuders et al. 2010.</p>
[STEPS]
?.
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71,132 | ADN PURIFICATION FROM AGAROSE GELS WITH GENELUTE GEL EXTRACTION KIT | 1 | dx.doi.org/10.17504/protocols.io.kxygx9p5dg8j/v1 | https://www.protocols.io/view/adn-purification-from-agarose-gels-with-genelute-g-chp4t5qw | Ana Belem García González, Georgina Elisa Diego Macías, Irán Alessandra Chaparro Rodríguez, Jair Alexis Gardea Sáenz | TITLE: ADN PURIFICATION FROM AGAROSE GELS WITH GENELUTE GEL EXTRACTION KIT
AUTHORS: Ana Belem García González, Georgina Elisa Diego Macías, Irán Alessandra Chaparro Rodríguez, Jair Alexis Gardea Sáenz
[DESCRIPTION]
Protocol for DNA purification from DNA purification from agarose gels with GeneElute Gel Extraction Ki... | ["Previous electrophoresis : Realization of an electrophoresis in a agarose gel of the product of ADN, charging the maximum amount of DNA per well (if necessary, use more than one well)", "Extraction of the band of interest: Extract the band of interest of the gel, removing the excess of agarose", "Weighting the band: ... |
43,717 | Validation of Tankyrase Binding and PARylation in Full-Length Protein Context | 5 | dx.doi.org/10.17504/protocols.io.bnxdmfi6 | https://www.protocols.io/view/validation-of-tankyrase-binding-and-parylation-in-bnxdmfi6 | Katie Pollock, Michael Ranes, Ian Collins, Sebastian Guettler | TITLE: Validation of Tankyrase Binding and PARylation in Full-Length Protein Context
AUTHORS: Katie Pollock, Michael Ranes, Ian Collins, Sebastian Guettler
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is part of a collection: </span><a href="https://www.protocols.io/private/DA... | ["[3.3 Validation of Tankyrase Binding and PARylation in Full-Length Protein Context]\nThis final section outlines a step-by-step approach to assess and validate the full-length candidate protein interaction with full-length tankyrase and the extent of its tankyrase-dependent PARylation. Mammalian expression constructs... |
32,707 | Infecting and mosquitoes with Dirofilaria immitis and Brugia malayi | null | dx.doi.org/10.17504/protocols.io.bb7birin | null | Michael Povelones, Abigail McCrea | TITLE: Infecting and mosquitoes with Dirofilaria immitis and Brugia malayi
AUTHORS: Michael Povelones, Abigail McCrea
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is for infecting </span><span style = "font-style:italic;">Aedes aegypti</span><span> and </span><span style = "fo... | ["[Counting microfilariae]\nDisperse or microfilaremic blood in a spot of water on a microscope slide. This will lyse red blood cells and make the microfilariae more conspicuous and easy to count.\n5 µl\n10 µl\n50 µl\nWater type is not important. We have used deionized water or ultrapure molecular biology grade wate... |
62,537 | TruKeto Shark Tank Reviews (Scam Exposed 2022) - Does It Really Work? | 3 | dx.doi.org/10.17504/protocols.io.dm6gpbx51lzp/v1 | https://www.protocols.io/view/truketo-shark-tank-reviews-scam-exposed-2022-does-b9bhr2j6 | TruKeto | TITLE: TruKeto Shark Tank Reviews (Scam Exposed 2022) - Does It Really Work?
AUTHORS: TruKeto
[DESCRIPTION]
https://www.eastbaytimes.com/2022/05/12/truketo-reviews-shark-tank-warning-tru-keto-pills-price-legit-website-for-sale/
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.pkmdku6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><u>Goal:</u></p>
<p>Immunohistochemistry (or IHC) is a method that allows demonstrating the presence and location of proteins in tissue sections.</p>
<p> </p>
<p>Day 1:</p>
<ol>
<li>Thaw the slides about 15 minutes.</li>
<li>Wash the slides 1 x 15 minutes in PBS with gentle a... | [] |
59,448 | LCM-NanoPOTS workflow for spatial proteome mapping | 1 | dx.doi.org/10.17504/protocols.io.bp2l6129kvqe/v1 | https://www.protocols.io/view/lcm-nanopots-workflow-for-spatial-proteome-mapping-b6ayrafw | Yumi Kwon, Ernesto S Nakayasu, WEI-JUN QIAN, Ying Zhu | TITLE: LCM-NanoPOTS workflow for spatial proteome mapping
AUTHORS: Yumi Kwon, Ernesto S Nakayasu, WEI-JUN QIAN, Ying Zhu
[DESCRIPTION]
Spatial proteomics holds great potential to transform our understanding of the role of cell populations and their spatial organizations in human tissues related to diseases. However... | ["[Laser capture microdissection (LCM)] Before cut and collection: Load DMSO as capturing media on the nanoPOTS chip", "[Laser capture microdissection (LCM)] Set the nanoPOTS robot to be ready (Temperature, humidity).", "[Laser capture microdissection (LCM)] Align the nanoPOTS chip.", "[Laser capture microdissection (L... |
57,072 | Protocols from: Evolutionary analyses of visual opsin genes in frogs and toads: diversity, duplication, and positive selection | 4 | dx.doi.org/10.17504/protocols.io.b3yqqpvw | https://www.protocols.io/view/protocols-from-evolutionary-analyses-of-visual-ops-b3yqqpvw | Ryan K Schott, Leah Perez, Matthew A Kwiatkowski, Vance Imhoff, Jennifer M Gumm | TITLE: Protocols from: Evolutionary analyses of visual opsin genes in frogs and toads: diversity, duplication, and positive selection
AUTHORS: Ryan K Schott, Leah Perez, Matthew A Kwiatkowski, Vance Imhoff, Jennifer M Gumm
[DESCRIPTION]
Protocols used to extract mRNA from frog retinas, create cDNA libraries, and ampl... | ["[RNA Extraction] Transfer sample into a 1.5 mLl collection tube.", "[RNA Extraction] Pipette off RNALATER.", "[RNA Extraction] Add 600 µL Buffer RLT.", "[RNA Extraction] Add 6 µL Beta-mercaptoethanol.", "[RNA Extraction] Disrupt tissue with sterile pestle.", "[RNA Extraction] Pipette into Qiashredder column. Spin 2 m... |
100,358 | RNA-Isolation from milk samples for virus analysis | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8342lmk/v1 | https://www.protocols.io/view/rna-isolation-from-milk-samples-for-virus-analysis-dd9e293e | Sudhir Bhatia, Gudrun Baersch | TITLE: RNA-Isolation from milk samples for virus analysis
AUTHORS: Sudhir Bhatia, Gudrun Baersch
[DESCRIPTION]
This protocol is about a method to isolate RNA from the milk samples to conduct the viral analysis, for example avian Influenza Virus H5N1, Measles, Zika Virus, Dengue Fever Virus etc. in human and other mamm... | ["Adjust the temperature of heat block.", "Add 300µl of Tube A (Lysis buffer I) and 15µl of Tube K (Proteinase K) to the 100µl milk sample in the tube.", "Incubate at 56°C for 20-30 minutes. Add to this 400µl of Tube G (Lysis buffer II).\nIncubate at 70°C for 5 minutes.", "Add to this sample 400µl of molecular ethanol ... |
null | null | null | dx.doi.org/10.17504/protocols.io.ipccdiw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The mtDNA fragment (15869-740) was amplified using primers for polymerase chain reaction (PCR) : L15869F and H719R (L15869 F 5' AAAATACTCAAATGGGCCTGTC 3', H719R 5' CGTGGTGATTTAGAGGGTGAAC 3'). The 20 µl PCR reactions contained 2.0 µl 5×buffer, 1.6 µl 2.5 mM dNTP mix, 0.8 µl ea... | [] |
34,854 | Development of an anatomic landmark-based measurement of the Achilles tendon | 1 | dx.doi.org/10.17504/protocols.io.beaejabe | https://www.protocols.io/view/development-of-an-anatomic-landmark-based-measurem-beaejabe | Daniel Urness | TITLE: Development of an anatomic landmark-based measurement of the Achilles tendon
AUTHORS: Daniel Urness
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Introduction</span></div><div class = "text-block">Achilles tendon ruptures (ATRs) occurred with an incidence o... | ["[Positioning]\n1. Position subject sitting upright with hip flexed, abducted, and externally rotated so the lateral aspect of the lower leg lays flat over the outline of the framing square2. Align the plantar aspect of the foot with the base of the framing square and the posterior aspect of the calf with the vertical... |
27,664 | RAT-ChIP - Restriction Assisted Tagmentation Chromatin Immunoprecipitation | null | dx.doi.org/10.17504/protocols.io.69qhh5w | null | Tonis Org, Kati Hensen, Rita Kreevan, Elina Mark, Olav Sarv, Reidar Andreson, Ülle Jaakma, Andres Salumets, Ants Kurg | TITLE: RAT-ChIP - Restriction Assisted Tagmentation Chromatin Immunoprecipitation
AUTHORS: Tonis Org, Kati Hensen, Rita Kreevan, Elina Mark, Olav Sarv, Reidar Andreson, Ülle Jaakma, Andres Salumets, Ants Kurg
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Chromatin immunoprecipitation coupled with... | ["[Bind antibodies to protein G Dynabeads]\n- Pipet 1µl of protein G Dynabeads per IP into standard 0.2ml eppendorf tube containing 50 µl of IP buffer at RT. - Capture the magnetic beads by 1 min incubation on a magnetic stand (Diagenode) and careful removal of the buffer.- Take beads up in, 2 times the volume of beads... |
56,298 | Effect of lumbar lordosis angle in adolescent baseball players on the development of lumbar spondylolysis | 1 | dx.doi.org/10.17504/protocols.io.b28iqhue | https://www.protocols.io/view/effect-of-lumbar-lordosis-angle-in-adolescent-base-b28iqhue | Yuki Onoda, Takashi Kitagawa | TITLE: Effect of lumbar lordosis angle in adolescent baseball players on the development of lumbar spondylolysis
AUTHORS: Yuki Onoda, Takashi Kitagawa
[DESCRIPTION]
Abstract
Background: Lumbar spondylolysis is a stress fracture of the lumbar vertebral arch that occurs frequently in adolescents. Lumbar spondylolysis... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ebmbak6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Fresh Protein Extraction Sample Buffer.
[STEPS]
?.
?.
?.
?.
?. | [] |
18,556 | UMass - Coronary Artery Ligation | null | dx.doi.org/10.17504/protocols.io.wc4fayw | null | Richard McIndoe, Mark Kelly, Timothy P. Fitzgibbons | TITLE: UMass - Coronary Artery Ligation
AUTHORS: Richard McIndoe, Mark Kelly, Timothy P. Fitzgibbons
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a mouse model of acute myocardial infarction. This protocol is suitable for studying mouse models thought to be relevant to coronary ischemia o... | ["Anesthetize the mouse and ensure depth of anesthesia with a toe pinch.", "Shave the left thoracic area of the mouse.", "The mouse is intubated using a 22-G angiocatheter sheath and placed on a rodent ventilator with the left chest up, secure feet to surgical area with tape.", "Prep the surgical field with 70% isoprop... |
69,838 | Impact of Internet-based Educational Intervention for Environmental Health Behavior among Pregnant Women | 3 | dx.doi.org/10.17504/protocols.io.e6nvwj6jwlmk/v1 | https://www.protocols.io/view/impact-of-internet-based-educational-intervention-cgfnttme | ghjeong | TITLE: Impact of Internet-based Educational Intervention for Environmental Health Behavior among Pregnant Women
AUTHORS: ghjeong
[DESCRIPTION]
None
[STEPS] | [] |
29,437 | pAmpATPBChl Plasmid Map | null | dx.doi.org/10.17504/protocols.io.8y5hxy6 | null | Isabel Nimmo, Ellen Nisbet, Christopher Howe | TITLE: pAmpATPBChl Plasmid Map
AUTHORS: Isabel Nimmo, Ellen Nisbet, Christopher Howe
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Sequence for the pAmpATPBChl artificial minicircle</div></div>
[STEPS]
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.jxdcpi6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Satinsky, Brandon M., et al. 'Use of internal standards for quantitative metatranscriptome and metagenome analysis.' <em>Methods in enzymology</em> 531 (2012): 237-250.</p>
[GUIDELINES]
<p>DNA standards can be prepared by purchasing or extracting genomic DNA from a cultured ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kfdcti6 | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
24,345 | Flow Cytometry Analysis of Human Neutrophil Extracellular Traps in vitro | null | dx.doi.org/10.17504/protocols.io.3zzgp76 | null | Haruchika Masuda | TITLE: Flow Cytometry Analysis of Human Neutrophil Extracellular Traps in vitro
AUTHORS: Haruchika Masuda
[STEPS] | [] |
76,464 | Organoid Electroporation using CRISPR RNP method | 4 | dx.doi.org/10.17504/protocols.io.5qpvor74xv4o/v1 | https://www.protocols.io/view/organoid-electroporation-using-crispr-rnp-method-cnwqvfdw | mns | TITLE: Organoid Electroporation using CRISPR RNP method
AUTHORS: mns
[DESCRIPTION]
Organoid electroporation using ribonucleoprotein (RNP) CRISPR based approach for highly efficient genome editing.
[STEPS]
SECTION: Single Cell Dissociation (Day 0)
4. Remove the medium from the organoids and add 500 µL of TrypLE Expres... | ["[Single Cell Dissociation (Day 0)] Remove the medium from the organoids and add 500 µL of TrypLE Express supplemented with 10 μM Y-27632 to each well. Scrape the Matrigel off the bottom of the wells with a 1,000-μl pipette. Split the organoids in 2-4 15 mL Falcons to have smaller volume for the dissociation process."... |
89,535 | The efficacy of non-pharmaceutical treatment of menopausal musculoskeletal symptoms: A state-of-the-art scoping review protocol | 1 | dx.doi.org/10.17504/protocols.io.n92ldm7wnl5b/v1 | https://www.protocols.io/view/the-efficacy-of-non-pharmaceutical-treatment-of-me-c3n7ymhn | Delyth Wyndham, June Keeling | TITLE: The efficacy of non-pharmaceutical treatment of menopausal musculoskeletal symptoms: A state-of-the-art scoping review protocol
AUTHORS: Delyth Wyndham, June Keeling
[DESCRIPTION]
Objective:
The objective of this state-of-the-art scoping review is to examine the scope of the contemporary research literature e... | [] |
67,280 | Centriflaken: an automated data analysis pipeline for assembly and in silico analyses of food-borne pathogens from metagenomic samples | 5 | dx.doi.org/10.17504/protocols.io.kxygxzdbwv8j/v1 | https://www.protocols.io/view/centriflaken-an-automated-data-analysis-pipeline-f-cdxqs7mw | Kranti Konganti, Vishal Thovarai, Meghan Maguire, Julie A. Kase, Narjol Gonzalez-Escalona | TITLE: Centriflaken: an automated data analysis pipeline for assembly and in silico analyses of food-borne pathogens from metagenomic samples
AUTHORS: Kranti Konganti, Vishal Thovarai, Meghan Maguire, Julie A. Kase, Narjol Gonzalez-Escalona
[DESCRIPTION]
Rapid and comprehensive analysis of metagenomic data from any sa... | ["[Step 1] Create an account and Login:\n\nIf you do not already have an account on GalaxyTrakr, please create one by visiting this URL: https://account.galaxytrakr.org/Account/Register", "[Step 1] Once your account is activated, login by visiting https://galaxytrakr.org.", "[Step 2] Create a new history:\n \n\n \n\n\n... |
null | null | null | dx.doi.org/10.17504/protocols.io.d6m9c5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol describes the design of primer pairs against the human genome for the synthesis of probes for high-definition DNA FISH (HD-FISH). This pipeline selects PCR primer pairs with optimal thermodynamic features, delimiting amplicons 200–220 nucleotides in length, and fil... | [] |
100,114 | MetaAMRSpotter: Automated workflow with shell scripting for uncovering hidden AMR hotspots from metagenomes | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1jyzlmk/v1 | https://www.protocols.io/view/metaamrspotter-automated-workflow-with-shell-scrip-ddzs276e | Chandrashekar K, Anagha S Setlur, S Pooja, M Purushotham Rao, Vidya Niranjan | TITLE: MetaAMRSpotter: Automated workflow with shell scripting for uncovering hidden AMR hotspots from metagenomes
AUTHORS: Chandrashekar K, Anagha S Setlur, S Pooja, M Purushotham Rao, Vidya Niranjan
[DESCRIPTION]
This protocol employs a novel, open-source automated pipeline scripted entirely in shell for analyzing m... | ["[DIRECTORY SPECIFICATION AND UNZIPPING FILES] Specify the directory of the file and unzip all .gz files in the directory. Check if the file is found first and if yes, then proceed to the next step.\n\nCode provided below:", "[RUNNING FASTQC] This tool describes the quality of the raw sequence data which is a result o... |
52,103 | Change in treatment burden among people with multimorbidity: protocol of a follow up survey and development of efficient measurement tools for primary care. | 1 | dx.doi.org/10.17504/protocols.io.bw5fpg3n | https://www.protocols.io/view/change-in-treatment-burden-among-people-with-multi-bw5fpg3n | H.O.Hounkpatin , *, Paul Roderick, James E Morris, James E Morris, Scott Harris, Forbes Watson, Hajira Dambha-Miller, Helen Roberts | TITLE: Change in treatment burden among people with multimorbidity: protocol of a follow up survey and development of efficient measurement tools for primary care.
AUTHORS: H.O.Hounkpatin , *, Paul Roderick, James E Morris, James E Morris, Scott Harris, Forbes Watson, Hajira Dambha-Miller, Helen Roberts
[DESCRIPTION]
... | [] |
53,759 | Construction of Mutant Library | 4 | dx.doi.org/10.17504/protocols.io.byq7pvzn | https://www.protocols.io/view/construction-of-mutant-library-byq7pvzn | Shuning Guo | TITLE: Construction of Mutant Library
AUTHORS: Shuning Guo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to construct mutant library of target gene with high efficiency and low false positives/negatives rate after subsequent functional screening.</div></div>
[STEPS]
?. [Err... | ["[Error-prone PCR]\nAdd the following reagent to a PCR tube (50μl) (Random Mutagenesis Kit by Solarbio).Template(10μl)Depends on the concentrationForward Primer (10 μM)1μlReverse Primer (10 μM)1μlMut Enhencer3μl2 x Mut Random System25μlddH2OAdd to 50μl\nTemplate(10μl)Depends on the concentrationForward Primer (10 μM)1... |
null | null | null | dx.doi.org/10.17504/protocols.io.naudaew | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Mannitol oxidase is an enzyme present in some gastropods, converting the polyalcohol mannitol into the sugar mannose. Molecular oxygen is the hydrogen acceptor and hydrogen peroxide is produced. Therefore, this enzymatic activity can be measured by monitoring H<sub>2</sub>O<s... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.uz7ex9n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
The following protocol outlines aliquoting resuspended primers, stored at 100µM in stock plates, into working primer plates at a conentration of 5µM for both forward (barcoded) and reverse primers, using the epMotion 5075. The Knigh Lab stores primer combos premixed in working p... | ["[Prepare reagents] Resuspend Reverse Primer from lyophilized sample vial to a final concentration of 100 µM", "[Prepare reagents] {\"blocks\":[{\"key\":\"483ad\",\"text\":\"In a sterile 30mL reservoir, add 15,840 \\u00b5L of PCR Clean Water and 880 \\u00b5L of Reverse Primer (100 \\u00b5M) to make (4) 96-Well primer ... |
49,872 | WIPI2d Expression and purification | 4 | dx.doi.org/10.17504/protocols.io.buxqnxmw | https://www.protocols.io/view/wipi2d-expression-and-purification-buxqnxmw | lmstrong | TITLE: WIPI2d Expression and purification
AUTHORS: lmstrong
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">WIPI2d expression and purification from HEK cells </div></div>
[STEPS]
?. [Expression]
Transfect of HEK GNTI cells
300 mL
?. [Purification]
Resuspended pellet in 25mL of lysis buffer (50mM... | ["[Expression]\nTransfect of HEK GNTI cells\n300 mL", "[Purification]\nResuspended pellet in 25mL of lysis buffer (50mM Hepes pH 7.45, 300mM NaCl, 1mM TCEP, 1% Triton X, PI cocktail)", "[Expression]\nDilute PEI with Warm Hybridoma-SFM(1X)", "[Purification]\nLet rock at for\n4 °C", "[Purification]\nClarify lysate for\... |
null | null | null | dx.doi.org/10.17504/protocols.io.jhmcj46 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
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55,422 | 2021-11-25 - Plasma Ultracentrifugation Protocol | 1 | null | https://www.protocols.io/view/2021-11-25-plasma-ultracentrifugation-protocol-b2c6qaze | Dakota Gustafson | TITLE: 2021-11-25 - Plasma Ultracentrifugation Protocol
AUTHORS: Dakota Gustafson
[DESCRIPTION]
Here we describe a bench top protocol for the isolation of extracellular vesicles (EVs) from human plasma. Plasma and other body fluids contain membranous EVs, which are considered to derive from a wide variety of cells. E... | ["[REAGENT AND SAMPLE SETUP] Prepare all materials needed for the protocol.", "[REAGENT AND SAMPLE SETUP] Annotate samples and tubes with corresponding sample IDs.", "[REAGENT AND SAMPLE SETUP] Thaw samples on wet ice for two hours with inversion of tubes every thirty minutes. Sample input should be at least 1mL.", "[R... |
43,929 | Syngenta divergent strain screen protocol | 4 | dx.doi.org/10.17504/protocols.io.bn5zmg76 | https://www.protocols.io/view/syngenta-divergent-strain-screen-protocol-bn5zmg76 | Ida Barlow, Adam Mcdermott-Rouse, Luigi Feriani | TITLE: Syngenta divergent strain screen protocol
AUTHORS: Ida Barlow, Adam Mcdermott-Rouse, Luigi Feriani
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Protocol for screening the 12 divergent </span><span style = "font-style:italic;">C. elegans</span><span> strains with 100 Syngenta pesticid... | ["[Pick L4 worms for bleaching (-9 days from first day of tracking)]\nPick 10 x L4s for 6 strains onto 3 x 90mm plates (pre-seeded with OP50) for each strain", "[Pick L4 worms for bleaching and pour 96WPs (-7 days)]\nPick 10 x L4s for 6 strains onto 3 x 90mm plates (pre-seeded with OP50) for each strain", "[Pick L4 wor... |
68,691 | DNA extraction for long-read sequencing of bacteria | 4 | dx.doi.org/10.17504/protocols.io.4r3l2ox24v1y/v1 | https://www.protocols.io/view/dna-extraction-for-long-read-sequencing-of-bacteri-cfbttinn | Eby Sim | TITLE: DNA extraction for long-read sequencing of bacteria
AUTHORS: Eby Sim
[DESCRIPTION]
Utilisation of long-read sequencing can reliably generate complete bacterial genomes. Here, we present a DNA extraction method which introduces minor modifications to the DNeasy® UltraClean® Microbial Kit (Qiagen) to generate DN... | ["[General bacteria culture] Streak bacteria of interest onto their respective, optimum solid media and incubate plates at optimal growth conditions.", "[General bacteria culture] On the day of DNA extraction, observe the plate to ensure purity. Do not attempt extraction if different colony morphologies are observed.",... |
109,157 | Evaluation of Micro-Tensile Bond Strength to Dentin, Failure mode, and Fluoride release of GDMA-Based Versus HEMA-Based Universal Adhesives after Aging: An in vitro study | 0 | dx.doi.org/10.17504/protocols.io.81wgbz32ygpk/v1 | https://www.protocols.io/view/evaluation-of-micro-tensile-bond-strength-to-denti-dnud5es6 | Aya Ibrahim tarek | TITLE: Evaluation of Micro-Tensile Bond Strength to Dentin, Failure mode, and Fluoride release of GDMA-Based Versus HEMA-Based Universal Adhesives after Aging: An in vitro study
AUTHORS: Aya Ibrahim tarek
[DESCRIPTION]
The aim of the present study is to evaluate the micro-tensile bond strength to dentin, failure mode,... | ["[Samples, interventions and outcomes] Sample size calculation", "[Samples, interventions and outcomes] Sample preparation\ntotal of 50 freshly extracted caries-free human molars will be used in this study. The occlusal enamel will be removed at 90º to the long axis of the tooth by means of a model trimmer under runni... |
63,601 | K1 keto - (Top Weight Loss Supplement) Quickly Work !!*SPECIAL OFFER* | 3 | dx.doi.org/10.17504/protocols.io.j8nlkk235l5r/v1 | https://www.protocols.io/view/k1-keto-top-weight-loss-supplement-quickly-work-sp-cacrsav6 | Advanced Appetite | TITLE: K1 keto - (Top Weight Loss Supplement) Quickly Work !!*SPECIAL OFFER*
AUTHORS: Advanced Appetite
[DESCRIPTION]
K1 Keto Life Reviews- 100% Legit Weight Loss Supplement With Quick Results! Get you into ketosis in minutes without the need to follow a severe eating routine.
(Buy Now)=>> https://reviewbylogix.onli... | [] |
30,708 | RNA extraction_Trizol method_Protocol | 1 | dx.doi.org/10.17504/protocols.io.98uh9ww | https://www.protocols.io/view/rna-extraction-trizol-method-protocol-98uh9ww | Rui Zhang, Clara Huesing, Heike Muenzberg | TITLE: RNA extraction_Trizol method_Protocol
AUTHORS: Rui Zhang, Clara Huesing, Heike Muenzberg
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The study goal is to identify the gene expression profile of interscapular brown fat (iBAT)-related ganglia (SG/T1 & T3) and inguinal white fat (Iwat)-relat... | ["Take out the samples from the -80 °C freezer and keep them stay in the ice.", "Add 500 µl Trizol (Ambion® by Life Technologies, Catalog number: 15596-026) to each sample (when adding the Trizol, add it toward the tissue so as to make sure the tissue is in the Trizol solution, instead of sticking on the tube well).", ... |
6,524 | Cold Spicy Sichuan Noodles | null | dx.doi.org/10.17504/protocols.io.ik4ccyw | null | Hao Ye | TITLE: Cold Spicy Sichuan Noodles
AUTHORS: Hao Ye
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Inspired by Ba Ren (RIP), sour, a little sweet, numbing spiciness. I added in some lightly-pickled cucumbers for extra crunch.</div></div>
[STEPS]
?. [Pickled Cucumbers]
Prepare pickled cucumbers follo... | ["[Pickled Cucumbers]\nPrepare pickled cucumbers following linked recipe.Brine ingredients: AB11 cuprice vinegar (unseasoned)21 cupwater33/4 cupsugar41 tbspsaltOther ingredients: AB16persian cucumbers (julienned)Notes:We are going to combine these cucumbers with the noodles, so keep that in mind when determining how ... |
54,193 | Preparation of BG-11 media Agar | 4 | dx.doi.org/10.17504/protocols.io.by6rpzd6 | https://www.protocols.io/view/preparation-of-bg-11-media-agar-by6rpzd6 | Ashwinuday | TITLE: Preparation of BG-11 media Agar
AUTHORS: Ashwinuday
[DESCRIPTION]
This is for making solid media of BG-11
[STEPS]
1. To make BG-11 Agar plates, add Agar-Agar Type-1 equivalent to 1.5% (w/v) of liquid media and autoclave.
2. Then before it solidifies (when the flask is bearable to touch), add the required an... | ["To make BG-11 Agar plates, add Agar-Agar Type-1 equivalent to 1.5% (w/v) of liquid media and autoclave.", "Then before it solidifies (when the flask is bearable to touch), add the required antibiotics to the media.", "Pour ~30-40 ml into plates soon after.", "Keep the plates opened in the hood.", "Once it solidifies ... |
34,647 | PlOSONE folder | null | dx.doi.org/10.17504/protocols.io.bd3xi8pn | null | Sung-Jin Bae, Inah Kim, Jaechul Song, Euy-Suk Chung | TITLE: PlOSONE folder
AUTHORS: Sung-Jin Bae, Inah Kim, Jaechul Song, Euy-Suk Chung
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;font-style:italic;">Background</span></div></div><div class = "text-block"><div class = ... | [] |
78,364 | Effects of Online Exercise Intervention on Physical and Mental Conditions in Young Adults with Chronic Neck Pain | 2 | dx.doi.org/10.17504/protocols.io.5qpvordnbv4o/v1 | https://www.protocols.io/view/effects-of-online-exercise-intervention-on-physica-cqr4vv8w | Yiting Lin, Raymond Tsang, Jinghua Qian | TITLE: Effects of Online Exercise Intervention on Physical and Mental Conditions in Young Adults with Chronic Neck Pain
AUTHORS: Yiting Lin, Raymond Tsang, Jinghua Qian
[DESCRIPTION]
Objectives
This study aimedto compare the efficacy of online exercise therapy with conventional exercise therapy on pain, function, psyc... | [] |
51,616 | DIDA-Seq Custom Capture cfDNA Library Preparation | 4 | dx.doi.org/10.17504/protocols.io.bwm8pc9w | https://www.protocols.io/view/dida-seq-custom-capture-cfdna-library-preparation-bwm8pc9w | Christopher T Boniface | TITLE: DIDA-Seq Custom Capture cfDNA Library Preparation
AUTHORS: Christopher T Boniface
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;:UNDERLINE;">D</span><span style = "font-weight:bold;">ual </span><span style = "font-weight:bold;:UNDERLINE;">I</span><span style ... | ["[cfDNA Isolation]\nUsing 3-10 mL of plasma (as needed, see note below) and NucleoSnap DNA Plasma Kit (Macherey-Nagel), follow manufacturer’s protocol to isolate cfDNA and elute with 50 ul nH2O or TE-low. Store at 4C for several days or freeze for long-term storage.\nYield is highly subject to the physiological state ... |
21,846 | DNA isolation from Formalin-Fixed, Paraffin-Embedded (FFPE) material | null | dx.doi.org/10.17504/protocols.io.zjwf4pe | null | Hendrik F van Essen | TITLE: DNA isolation from Formalin-Fixed, Paraffin-Embedded (FFPE) material
AUTHORS: Hendrik F van Essen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the isolation of DNA from paraffin embedded tissue. The resulting DNA can be used for CGH array hybridizations. </div><div ... | ["[H&E staining from paraffin blocks]\nCut 3 µm (1x), 10 µm (2-3x, or more if needed depending on tumour area)", "[H&E staining from paraffin blocks]\nMount all the section on slides with BSA 0.1%, and dry them o/n at 37°C (to a maximum of one week)", "[H&E staining from paraffin blocks]\nPlace slides in xylene\n[3 ti... |
51,384 | Mobile Phone Spectrophotometer Setup [Tea experiment version] | 1 | dx.doi.org/10.17504/protocols.io.bweypbfw | https://www.protocols.io/view/mobile-phone-spectrophotometer-setup-tea-experimen-bweypbfw | Katharine E Hubbard | TITLE: Mobile Phone Spectrophotometer Setup [Tea experiment version]
AUTHORS: Katharine E Hubbard
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol allows you to turn a mobile phone (smart phone) into a colorimeter/spectrophotometer. It goes through how to set up your phone and workspace... | ["[Setting up your phone]\nInstall a colour picker app on your phone - you need one that gives you numerical Red Green Blue (RGB) values. I recommend 'ColorPicker' for iPhones, and 'ColorGrab' for Android.", "[Setting up your phone]\nFully charge your phone! Unplug your phone from it's charger and put the charger away ... |
53,235 | Quantification of nonprotein sulfhydryl groups (NPSH) optimized for zebrafish brain tissue | 6 | dx.doi.org/10.17504/protocols.io.bx8tprwn | https://www.protocols.io/view/quantification-of-nonprotein-sulfhydryl-groups-nps-bx8tprwn | Adrieli Sachett, Matheus Gallas-Lopes, Greicy M M Conterato, Radharani , Ana Herrmann, Angelo Piato | TITLE: Quantification of nonprotein sulfhydryl groups (NPSH) optimized for zebrafish brain tissue
AUTHORS: Adrieli Sachett, Matheus Gallas-Lopes, Greicy M M Conterato, Radharani , Ana Herrmann, Angelo Piato
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Zebrafish are incresingly used as a model ani... | ["[Preparing the reagents]\nThe first step is to prepare the reagents to be used in the quantification of nonprotein sulfhydryl groups (NPSH);", "[Preparing the reagents]\n5,5′-Dithiobis(2-nitrobenzoic acid) (DTNB) : 1.1.1 Weigh carefully of DTNB in a piece of aluminum foil; 1.1.2 Transfer the DTNB to a beaker o... |
33,521 | Cell Biology | null | dx.doi.org/10.17504/protocols.io.bcyrixv6 | null | Ken Christensen | TITLE: Cell Biology
AUTHORS: Ken Christensen
[STEPS] | [] |
89,679 | Set Shifting ASAP Operant Behavior_LernerLab | 1 | dx.doi.org/10.17504/protocols.io.q26g7p3n9gwz/v1 | https://www.protocols.io/view/set-shifting-asap-operant-behavior-lernerlab-c3tpynmn | jillian.seiler, jacobnadel, talia.lerner | TITLE: Set Shifting ASAP Operant Behavior_LernerLab
AUTHORS: jillian.seiler, jacobnadel, talia.lerner
[DESCRIPTION]
Protocol for set shifting task used by the Lerner Lab for ASAP work
[STEPS]
SECTION: Magazine Training
1. Food restrict animals overnight prior to beginning magazine training. For the remainder of train... | ["[Magazine Training] Food restrict animals overnight prior to beginning magazine training. For the remainder of training animals should be restricted to 85% of their free feeding weight.", "[Magazine Training] Place animals in operant box with dummy patch cords (if fiber photometry or optogenetics will be used) attach... |
null | null | null | dx.doi.org/10.17504/protocols.io.vcne2ve | null | null | TITLE: No Title
AUTHORS:
[STEPS]
SECTION: Culture condition of O. marina
?.
SECTION: Concentration of O. marina cells
?.
SECTION: Electroporation with Bio-Rad Gene Pulser Xcell
?. | ["[Culture condition of O. marina] Transfer Oxyrrhis marina (NIES-494) cells to 20 mL of fresh IMK medium (Nihon Pharmaceutical Co., Ltd.) in a plastic flask (IWAKI 75 cm2) at the concentration of 200 cells/mL, and add Pyramimonas parkeae (NIES-254) as feed at the concentration of 1×104 cells/mL.\n \nGrow cells at 22℃ ... |
90,143 | Village Nuclei Isolation With Myelin Removal | 4 | dx.doi.org/10.17504/protocols.io.4r3l22e3xl1y/v1 | https://www.protocols.io/view/village-nuclei-isolation-with-myelin-removal-c397yr9n | Steve McCarroll, Emi Ling, Melissa Goldman, Nora Reed | TITLE: Village Nuclei Isolation With Myelin Removal
AUTHORS: Steve McCarroll, Emi Ling, Melissa Goldman, Nora Reed
[DESCRIPTION]
Isolation of nuclei from fresh-frozen brain tissue from sets of multiple (typically 2-20) human donors for analysis as a “cell village” (Wells et al., PMID 36796362) in which nuclei from all... | ["[Before Starting] Gather Supplies\nRazor Blades\nGlass slides\nSyringes with needles (3 mL syringe with 26 1⁄2 gauge needle)\nMyelin removal beads (cat # 130-096-731) https://www.miltenyibiotec.com/US-en/products/myelin-removal-beads-ii-human-mouse-rat.html#gref \nEppendorf tubes (1.5 mL and 5 mL)\nEppendorf or Raini... |
28,716 | Chitin binding + Bradford assay | null | dx.doi.org/10.17504/protocols.io.8akhscw | null | Robert Hooftman | TITLE: Chitin binding + Bradford assay
AUTHORS: Robert Hooftman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol can be used to measure the binding capacity of chitin binding proteins and visualizing this using a Bradford assay. The protocol has been adapted from:</div><div class = "tex... | ["[Chitin binding assay]\nFirst, prepare the following buffer:Chitin buffer ABC1KH2PO4 2 mM (0,272 g/L) 2Na2HPO4 8 mM (1,424 g/L in case of Na2HPO4 * 2 H2O) 3KCl 2 mM (0,149 g/L) Preparation of chitin solution. Make a chitin stock of 10 mg/ml in the ... |
78,209 | Decellularized adipose tissue solid foams for hDPSC differentiation to osteogenic and adipogenic cells in 3D culture | 4 | dx.doi.org/10.17504/protocols.io.81wgbyk8ovpk/v1 | https://www.protocols.io/view/decellularized-adipose-tissue-solid-foams-for-hdps-cqk9vuz6 | Gaskon Ibarretxe | TITLE: Decellularized adipose tissue solid foams for hDPSC differentiation to osteogenic and adipogenic cells in 3D culture
AUTHORS: Gaskon Ibarretxe
[DESCRIPTION]
Human Dental Pulp Stem Cells (hDPSCs) constitute one very interesting stem cell source for tissue engineering and regeneration, owing to their high multi-l... | ["Decellularized adipose tissue solid foams for hDPSC differentiation to osteogenic and adipogenic cells in 3D culture"] |
28,950 | Latex beads migration assay test | null | dx.doi.org/10.17504/protocols.io.8hwht7e | null | Jorge Fernández | TITLE: Latex beads migration assay test
AUTHORS: Jorge Fernández
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The following protocol details how to test the migration of conjugated latex beads trough different nitrocellulose membranes.</div></div>
[STEPS]
?. [Membrane Preparation]
Prepare two FF... | ["[Membrane Preparation]\nPrepare two FF170HP strips and three FF80HP strips of 1cm wide x 4 cm long.Wax print the microfluidic membranes following the protocol mentioned in the guideliness section.", "[Membrane Preparation]\nBlock one FF170HP and two FF80HP membranes by immersion in 0.2 % BSA solution in PBS. Let the... |
73,785 | Build your own calculus | 2 | dx.doi.org/10.17504/protocols.io.dm6gpj9rdgzp/v1 | https://www.protocols.io/view/build-your-own-calculus-ckazusf6 | Bjørn Peare Bartholdy, a.g.henry | TITLE: Build your own calculus
AUTHORS: Bjørn Peare Bartholdy, a.g.henry
[DESCRIPTION]
This collection contains protocols for growing a calcifying oral biofilm model. Protocols include artificial saliva, a mineralising solution (CPMU), an alpha-amylase activity assay, and a biofilm growth protocol with starch treatme... | [] |
49,518 | Nuclei Isolation from Tissue for 10x snRNA | 4 | null | https://www.protocols.io/view/nuclei-isolation-from-tissue-for-10x-snrna-buknnuve | Minyi Shi, Annika Weimer | TITLE: Nuclei Isolation from Tissue for 10x snRNA
AUTHORS: Minyi Shi, Annika Weimer
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Nuclei Isolation from Tissue for 10x snRNA</div></div>
[STEPS]
?. [Stock Buffers]
?. [Before you start the protocol:]
1)All steps should be performed on ice or at 4°C.... | ["[Stock Buffers]", "[Before you start the protocol:]\n1)All steps should be performed on ice or at 4°C. Pre-chill a swinging bucket centrifuge and a fixed angle centrifuge to 4°C.2)Pre-chill all Dounces and pestles to 4°C in a fridge.3)Pre-chill all tubes. For each sample you are processing, you will need:a.One 2 ml r... |
60,447 | An X-HTDC method for estimating particulate phosphorus from microalgae | 6 | dx.doi.org/10.17504/protocols.io.kqdg35dq7v25/v5 | https://www.protocols.io/view/an-x-htdc-method-for-estimating-particulate-phosph-b697rh9n | Yingyu Hu, Zoe V. Finkel | TITLE: An X-HTDC method for estimating particulate phosphorus from microalgae
AUTHORS: Yingyu Hu, Zoe V. Finkel
[DESCRIPTION]
Total particulate phosphorus (TPP) is often determined using the High Temperature Dry Combustion (HTDC) method followed by hydrolysis of the ash and then molybdenum colorimetry. Here we show t... | ["[Sampling] Sampling microalgae for total particulate phosphorus (i.e. intracellular phosphorus and adsorbed phosphorus)", "[Sampling] Filter microalgae in liquid media onto polycarbonate filters, using gentle vacuum pressure (130 mmHg).", "[Sampling] Rinse samples with filtered seawater", "[Sampling] Place sample fil... |
20,662 | Feasibility of a connected interface implementation to foster the continuity of inpatient care: A pilot protocol for physicians handovers, in a tertiary maternity | null | dx.doi.org/10.17504/protocols.io.yewftfe | null | José Carlos Serufo Filho, Denise Utsch Gonçalves, Vitor Barbosa Abrantes, Bruna Barbosa Coimbra, Zilma Reis | TITLE: Feasibility of a connected interface implementation to foster the continuity of inpatient care: A pilot protocol for physicians handovers, in a tertiary maternity
AUTHORS: José Carlos Serufo Filho, Denise Utsch Gonçalves, Vitor Barbosa Abrantes, Bruna Barbosa Coimbra, Zilma Reis
[DESCRIPTION]
<div class = "text... | ["[Methods]\nSettingThe scenario of this study is tertiary care university-affiliated maternity, with 2900 births-per-year and 31 beds. A staff of novice and senior physicians gathers five doctors working 12 hours on duty; one of them is the leader in charge. Verbal and written handovers occur twice a day, at 7 am and ... |
11,444 | El-cheep-o miniprep | null | dx.doi.org/10.17504/protocols.io.peudjew | null | Magdalena Julkowska | TITLE: El-cheep-o miniprep
AUTHORS: Magdalena Julkowska
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol for cheep miniprep isolation. Be aware that the DNA quality might not be sufficient to use this protocol for cloning purposes. It works very well for the digestion analysis thou... | ["·Spin 3ml of the overnight culture to pellet cells (1.5-ml for time) @13000 rpm for 5 min", "·Remove the supernatant", "·Add 250 ml of sol P1+Rnase 5ml and vortex to resuspend cells", "·Take 15 min @ room temperature", "·Add 250 ml of sol P2 and mix gently", "·Take 5 min @ room temperature", "·Add 350 ml of sol P3 an... |
null | null | null | dx.doi.org/10.17504/protocols.io.rv8d69w | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
40,262 | Salmonella purification | 4 | dx.doi.org/10.17504/protocols.io.bjjekkje | https://www.protocols.io/view/salmonella-purification-bjjekkje | Angel Justiz-Vaillant, Suzette E Curtello | TITLE: Salmonella purification
AUTHORS: Angel Justiz-Vaillant, Suzette E Curtello
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The United States veterinarian Daniel E Salmon discovered the genus </span><span style = "font-style:italic;">Salmonella</span><span>. </span><span style = "font-s... | ["A 1:9 Salmonella suspension was made in buffered peptone water and incubated overnight at 37ºC.", "One ml of pre-enrichment broth was transferred to a 1.5 ml micro-centrifuge tube and centrifuged for ten minutes at 14,000 x g (Eppendorf Model 5424).", "The supernatant was carefully discarded.", "The pellets were re-s... |
44,412 | Antigen Presentation Protocol | 4 | dx.doi.org/10.17504/protocols.io.bpk4mkyw | https://www.protocols.io/view/antigen-presentation-protocol-bpk4mkyw | Lilia Rodriguez | TITLE: Antigen Presentation Protocol
AUTHORS: Lilia Rodriguez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details methods for 3-day Antigen Presentation Assay.</div></div>
[STEPS]
?. [Day 1]
Prepare a 96 well plate:Label appropriately (experimental condition – LPS/IFNg controls, i... | ["[Day 1]\nPrepare a 96 well plate:Label appropriately (experimental condition – LPS/IFNg controls, infection controls, etc. ) Duplicate labelled wells for the peptide control", "[Day 1]\nScrape RAW cells and resuspend (pipette up and down 8x).", "[Day 1]\nCount RAW cells and adjust concentration to 0.75 million cells/... |
84,417 | General Fungal DNA Extraction | 4 | null | https://www.protocols.click/view/general-fungal-dna-extraction-cwn9xdh6 | Angie Macias, Matthew T Kasson, Brian Lovett | TITLE: General Fungal DNA Extraction
AUTHORS: Angie Macias, Matthew T Kasson, Brian Lovett
[DESCRIPTION]
This is a routine protocol for extracting DNA from various fungi. This extraction method is suitable for follow-up molecular work such as PCR amplification.
[STEPS]
SECTION: Before you begin
1. Turn on hot water b... | ["[Before you begin] Turn on hot water bath, set to 65 °C.", "[Before you begin] Pull two Eppendorf 1.5 mL centrifuge tubes per sample.", "[Before you begin] Label both sets of tubes with (short) sample names.", "[Before you begin] Label one tube set for each sample with an \"I\" for .", "[Before you begin] Add 200 µL... |
null | null | null | dx.doi.org/10.17504/protocols.io.rspd6dn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>In order to take chlorophyll fluroscence images of cowpea accessions, leaf disks need to be collected and placed randomly onto a leaf disk tray. This protocol explains how.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
62,207 | Power Keto Gummies Reviews - Fake Side Effects Scam? | 1 | dx.doi.org/10.17504/protocols.io.q26g743qqgwz/v1 | https://www.protocols.io/view/power-keto-gummies-reviews-fake-side-effects-scam-b8y7rxzn | keto-x-reviews | TITLE: Power Keto Gummies Reviews - Fake Side Effects Scam?
AUTHORS: keto-x-reviews
[DESCRIPTION]
Power Keto Gummies Reviews - Fake Side Effects Scam?
[STEPS]
SECTION: Power Keto Gummies Reviews - Fake Side Effects Scam? Power Keto Gummies give colorful medical benefits to help those who can not bear the discomfort... | ["[Power Keto Gummies Reviews - Fake Side Effects Scam? Power Keto Gummies give colorful medical benefits to help those who can not bear the discomfort of taking colorful emulsion- loaded specifics to get well and end their regular straits. Salutary inconsistency and a lack of physical exertion are the two biggest pitf... |
91,747 | Isolation and co-culture of nodose ganglion neurons | 4 | dx.doi.org/10.17504/protocols.io.eq2lyjqjrlx9/v1 | https://www.protocols.io/view/isolation-and-co-culture-of-nodose-ganglion-neuron-c5uby6sn | andrew.west, arpine.sokratian | TITLE: Isolation and co-culture of nodose ganglion neurons
AUTHORS: andrew.west, arpine.sokratian
[DESCRIPTION]
Protocol useful for co-cultured intestinal organoids from SNCAA53T mice with nodose ganglia neurons from Snca-/- mice that lack any α-synuclein expression. Though enteric ganglia might natively express low l... | ["[Isolation of Nodose Ganglion for Culture]", "[Isolation of Nodose Ganglion for Culture] Coat the wells of an 8 well chamber slide with 10ul growth factor reduced matrigel (Corning, #354230) and place into a 37C incubator to polymerize.", "[Isolation of Nodose Ganglion for Culture] Follow carotid artery down, removin... |
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