id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
|---|---|---|---|---|---|---|---|
51,363 | A Systematic Comparison of Protocols for Recovery of High-Quality RNA from Human Islets Extracted by Laser Capture Microdissection | 4 | dx.doi.org/10.17504/protocols.io.bwebpban | https://www.protocols.io/view/a-systematic-comparison-of-protocols-for-recovery-bwebpban | Chiara M. A. Cefalo, Teresa Mezza, Andrea Giaccari, Rohit N. Kulkarni | TITLE: A Systematic Comparison of Protocols for Recovery of High-Quality RNA from Human Islets Extracted by Laser Capture Microdissection
AUTHORS: Chiara M. A. Cefalo, Teresa Mezza, Andrea Giaccari, Rohit N. Kulkarni
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The isolati... | ["[Materials and Methods]\nTo compare the efficiency of different RNA extraction protocols in frozen human islet samples, we used LCM to collect a mean of 100 islets from pancreatic surgical specimens, obtained from non-diabetic or diabetic patients who had undergone partial pancreatectomy for an extra-pancreatic tumor... |
98,871 | A protocol for computerized quantitative analysis of nerve fibers, mast cells, enteric glial cells and the proximity of mast cells to the nerve fibers in 3D Images of human sigmoid mucosal biopsies | 0 | null | https://www.protocols.io/view/a-protocol-for-computerized-quantitative-analysis-dcsx2wfn | Tao Li, Pu-Qing Yuan, Yvette Taché | TITLE: A protocol for computerized quantitative analysis of nerve fibers, mast cells, enteric glial cells and the proximity of mast cells to the nerve fibers in 3D Images of human sigmoid mucosal biopsies
AUTHORS: Tao Li, Pu-Qing Yuan, Yvette Taché
[DESCRIPTION]
This protocol describes a step-by-step computational wor... | [] |
62,422 | Prima Keto UK | 1 | dx.doi.org/10.17504/protocols.io.x54v9ykrqg3e/v1 | https://www.protocols.io/view/prima-keto-uk-b87wrzpe | Guilherme Loss | TITLE: Prima Keto UK
AUTHORS: Guilherme Loss
[DESCRIPTION]
(Saving Today) Click Here To Get Prima Keto For The Lowest Price Right Now
So, what exactly are Prima Keto Pills?
This supplement is the go-to supplement when it comes to helping people lose weight and sculpt their bodies. Despite its relative newness, it h... | ["(Saving Today) Click Here To Get Prima Keto For The Lowest Price Right Now\n\nSo, what exactly are Prima Keto Pills?\n\nThis supplement is the go-to supplement when it comes to helping people lose weight and sculpt their bodies. Despite its relative newness, it has already significantly impacted the market. To put it... |
31,223 | A Comparison of the Performance of Disinfection Agents on Smallmouth Grunt (Haemulon chrysargyreum) Eggs | null | dx.doi.org/10.17504/protocols.io.baqxidxn | null | Tory Stoddard, Quint Heyder, Alyssa Leonardi, Andrew Saltzman | TITLE: A Comparison of the Performance of Disinfection Agents on Smallmouth Grunt (Haemulon chrysargyreum) Eggs
AUTHORS: Tory Stoddard, Quint Heyder, Alyssa Leonardi, Andrew Saltzman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"> Fish egg disinfection is a common practice in aquaculture facilit... | ["Separate eggs from aerated collection bucket using a 40μm siv.", "Pipette a few milliliters of eggs from the 40μm siv into a 10mL graduated cylinder.", "Prepare the disinfectant bath by filling the watch glass halfway with filtered seawater. Then, add 1mL of iodine per 1L of filtered seawater.", "Once the eggs have s... |
88,009 | noesypr1d_metab.nan | 5 | dx.doi.org/10.17504/protocols.io.x54v9p21pg3e/v2 | https://www.protocols.io/view/noesypr1d-metab-nan-cz7hx9j6 | NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison | TITLE: noesypr1d_metab.nan
AUTHORS: NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison
[DESCRIPTION]
This is a protocol for running the Bruker pulse program "noesypr1d".
[BEFORE_START]
This protocol assumes your sample is loaded, locked, tuned, and... | ["[Create a new dataset]", "[Create a new dataset] On the menu bar on TopSpin, click on\nStart → Create Dataset", "[Create noesypr1d experiment file] When Lock, Tune, Shim are complete proceed to the specific protocol for your desired pulseprogram(s).", "[Create a new dataset] A new window opens. On the right top bar, ... |
79,385 | Selection of buffer for the HPLC estimation of Posaconazole | 1 | dx.doi.org/10.17504/protocols.io.dm6gpj1k1gzp/v1 | https://www.protocols.io/view/selection-of-buffer-for-the-hplc-estimation-of-pos-crrzv576 | annamalai.rama | TITLE: Selection of buffer for the HPLC estimation of Posaconazole
AUTHORS: annamalai.rama
[DESCRIPTION]
Introduction: Posaconazole is a widely used antifungal drug, and its accurate quantification is essential for quality control and assessment of its pharmaceutical products. This study aimed to develop and validate ... | ["The selection of an appropriate buffer is critical for achieving optimal separation and analysis of target compounds in HPLC. The choice of buffer depends on various factors such as the pKa of the compound, the pH range of the buffer, and the compatibility with the stationary phase. For acidic compounds, a buffer wit... |
null | null | null | dx.doi.org/10.17504/protocols.io.qendtde | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><em>The MELD Project is an international collaboration aiming to create open-access, robust and generalisable tools for FCD detection. To this end, we will train a neural network classifier on MRI features from FCD patients from multiple centres worldwide.</em></p>
<p><strong... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.niadcae | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
86,214 | Preparing whole cell samples for immunoblot analysis | 4 | dx.doi.org/10.17504/protocols.io.e6nvwdj4dlmk/v1 | https://www.protocols.io/view/preparing-whole-cell-samples-for-immunoblot-analys-cyfextje | Louise Uoselis | TITLE: Preparing whole cell samples for immunoblot analysis
AUTHORS: Louise Uoselis
[DESCRIPTION]
Protocol for preparation of HeLa cell lysates for immunoblot analysis.
[STEPS]
1. Add an appropriate volume of 1x LDS Sample Buffer (ThermoFisher) to each sample.
2. Boil each sample at 99 °C with shaking at maximum spee... | ["Add an appropriate volume of 1x LDS Sample Buffer (ThermoFisher) to each sample.", "Boil each sample at 99 °C with shaking at maximum speed for 10 min", "Allow all samples to cool to Room temperature, and quickly centrifuge the samples to collect all liquid in the bottom of the tube. Vortex each sample for ~3 seconds... |
22,249 | Chlamydia trachomatis detection and gene expression protocols | null | dx.doi.org/10.17504/protocols.io.zyhf7t6 | null | Athumani M. Ramadhani, Tamsyn Derrick, David Macleod, Patrick Massae, Aiweda Malisa, Kelvin Mbuya, Tara Mtuy, William Makupa, Chrissy h Roberts, Robin L. Bailey, David C. W. Mabey, Martin J. Holland, Matthew J. Burton | TITLE: Chlamydia trachomatis detection and gene expression protocols
AUTHORS: Athumani M. Ramadhani, Tamsyn Derrick, David Macleod, Patrick Massae, Aiweda Malisa, Kelvin Mbuya, Tara Mtuy, William Makupa, Chrissy h Roberts, Robin L. Bailey, David C. W. Mabey, Martin J. Holland, Matthew J. Burton
[DESCRIPTION]
<div cla... | [] |
34,609 | Max's Test Protocol | null | dx.doi.org/10.17504/protocols.io.bd2ri8d6 | null | Max Marrone | TITLE: Max's Test Protocol
AUTHORS: Max Marrone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a fake protocol to play with protocols.io.</div></div>
[STEPS]
?. [Test]
This is step 1.
?. [Test]
This is step 2. | ["[Test]\nThis is step 1.", "[Test]\nThis is step 2."] |
44,763 | PMN- 05 Culture of Human PMN - IL-8 production | 4 | dx.doi.org/10.17504/protocols.io.bpx3mpqn | https://www.protocols.io/view/pmn-05-culture-of-human-pmn-il-8-production-bpx3mpqn | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | TITLE: PMN- 05 Culture of Human PMN - IL-8 production
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Published work using this protocol:</div><div class = "text-block">- A... | ["Isolate PMN according either to the protocol \"PMN- 01a\" or \"PMN- 01b\".", "Resuspend PMN at 10x106 cells/ml in complete culture medium (SOLUTION- 13).", "Use a number of tubes according to the experimental plan and to the substances to test.", "Tubes will be prepared to test resting IL-8 production (substances alo... |
25,065 | Glass bead transformation of Heterosigma akashiwo | null | dx.doi.org/10.17504/protocols.io.4qhgvt6 | null | Deepak Nanjappa and Kathryn Coyne | TITLE: Glass bead transformation of Heterosigma akashiwo
AUTHORS: Deepak Nanjappa and Kathryn Coyne
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol was developed for the glass bead-mediated transformation of </span><span style = "font-style:italic;">Heterosigma akashiwo</span><sp... | ["Harvest exponential growth Heterosigma akashiwo culture (1-2 x 106 cells mL-1) by centrifugation at 1500 rpm for 90 s in a 50 mL sterile screw-cap tube. Remove the supernatant by pipetting.", "Resuspend the cell pellet in a small volume of MAX Efficiency® Transformation Reagent for Algae (Invitrogen, Thermo Fischer S... |
null | null | null | dx.doi.org/10.17504/protocols.io.uaxesfn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Reproduction of the three species od crocodilians in the Amazonia, BRazil
[STEPS] | [] |
62,426 | Oprah Winfrey Keto Gummies Reviews, Does It Real Or Fraud? | 1 | dx.doi.org/10.17504/protocols.io.rm7vzyo68lx1/v1 | https://www.protocols.io/view/oprah-winfrey-keto-gummies-reviews-does-it-real-or-b872rzqe | keto-x-reviews | TITLE: Oprah Winfrey Keto Gummies Reviews, Does It Real Or Fraud?
AUTHORS: keto-x-reviews
[DESCRIPTION]
Oprah Winfrey Keto Gummies Reviews, Does It Real Or Fraud?
[STEPS]
SECTION: Oprah Winfrey Keto Gummies Reviews, Does It Real Or Fraud? Oprah Winfrey Keto – The Supplement for a Spare Shape and Slim Body! There&... | ["[Oprah Winfrey Keto Gummies Reviews, Does It Real Or Fraud? Oprah Winfrey Keto – The Supplement for a Spare Shape and Slim Body! There's a superb product out then in the request that we all know by the name that goes as the Oprah Winfrey Keto. This blog is a complete in- depth analysis of this outstanding as w... |
75,649 | DETERMINATION OF PHARMACOLOGICAL ACTIVITY OF BIOACTIVES IN ALLIUM SATIVUM USING COMPUTATIONAL ANALYSIS | 5 | dx.doi.org/10.17504/protocols.io.j8nlkwm95l5r/v1 | https://www.protocols.io/view/determination-of-pharmacological-activity-of-bioac-cm49u8z6 | Ouma stephen, Faith Muthoni, Richard Kagia | TITLE: DETERMINATION OF PHARMACOLOGICAL ACTIVITY OF BIOACTIVES IN ALLIUM SATIVUM USING COMPUTATIONAL ANALYSIS
AUTHORS: Ouma stephen, Faith Muthoni, Richard Kagia
[DESCRIPTION]
Both qualitative and quantitative studies were done in this study. Quantitative data was generated in form of binding energies when binding of ... | ["Determining possible targets \nBioactive agents in Allium sativum namely z-ajoene, e-ajoene, alliin, allicin, S-allyl-cysteine, diallyl sulphide, diallyl disulphide and diallyl trisulphide were searched in PubChem tool and their canonical smiles were copied. Swiss target prediction tool was opened where Canonical smi... |
92,394 | hsqc_metab.nan | 5 | dx.doi.org/10.17504/protocols.io.5jyl8pd2dg2w/v4 | https://www.protocols.io/view/hsqc-metab-nan-c6gizbue | NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison | TITLE: hsqc_metab.nan
AUTHORS: NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison
[DESCRIPTION]
This is a protocol for running the Bruker pulse program "hsqcetgpsisp2".
[BEFORE_START]
This protocol assumes:
Your sample is loaded, locked, tuned for ... | ["[Create a new dataset]", "[Create a new dataset] A new window opens. On the right top bar, select\nSource = /opt/NAN_METAB/par\n \nIn the list, select the one you want to use:\n\nFor serum and plasma samples:\nHSQC_br600_serum.par: Parameter set using an acquisition mode \"traditional planes\"\nHSQC_NUS_br600_serum.p... |
29,323 | METABOLIC PATHWAY ANALYSIS BY LIQUID CHROMATOGRAPHY (UHPLC) COUPLED TO HIGH RESOLUTION MASS SPECTROMETRY (LC/MS) | null | dx.doi.org/10.17504/protocols.io.8vjhw4n | null | Angelica S. Gouveia, Lucas L. Lima, Flaviane S. Coutinho, Juliano M. Rodrigues, Valquiria J. M. Pinheiro, Maria Eduarda S. Ramos, Camilo E. Vital, Claudia S.L. Pontes, Daniella P. Pinheiro, Pedro M. Vidigal, Edvaldo Barros, Humberto J O Ramos | TITLE: METABOLIC PATHWAY ANALYSIS BY LIQUID CHROMATOGRAPHY (UHPLC) COUPLED TO HIGH RESOLUTION MASS SPECTROMETRY (LC/MS)
AUTHORS: Angelica S. Gouveia, Lucas L. Lima, Flaviane S. Coutinho, Juliano M. Rodrigues, Valquiria J. M. Pinheiro, Maria Eduarda S. Ramos, Camilo E. Vital, Claudia S.L. Pontes, Daniella P. Pinheiro, P... | ["[DATA PROCESSING AND LC/MS ALIGNMENT]\n1) Install the Proteowizard package for your operational system (32 bits or 64 bits).2) Convert the data to mzXML format using ProteoWizard by executing MSConvertGUI.exe.3) Locate the directories to input all spectra and output; choose filters Peak Picking and MS level 1, click ... |
80,660 | Skin Biopsy Protocol (Mammals): Non-lethal Sampling | 1 | dx.doi.org/10.17504/protocols.io.n2bvj64nxlk5/v5 | https://www.protocols.io/view/skin-biopsy-protocol-mammals-non-lethal-sampling-cszuwf6w | sanaz.arenivas, justin_bohling, comizzolip, mhouck, Rachel A Johnston, Jacquelyn Mountcastle, Budhan Pukazhenthi, phil.purdy, brian_small, seth_willey | TITLE: Skin Biopsy Protocol (Mammals): Non-lethal Sampling
AUTHORS: sanaz.arenivas, justin_bohling, comizzolip, mhouck, Rachel A Johnston, Jacquelyn Mountcastle, Budhan Pukazhenthi, phil.purdy, brian_small, seth_willey
[DESCRIPTION]
Version date: April 2023
The following protocol illustrates how to collect and ship l... | ["[Preparation] Pre-chill icepacks in the freezer the day before planning to collect and ship samples.", "[Preparation] Record all information indicated in the biopsy form, including a picture of the animal for identification and GPS location where the animal was found.", "[Preparation] Proper protective equipment must... |
33,846 | Extraction Procedure | null | dx.doi.org/10.17504/protocols.io.bdawi2fe | null | Jing Xu | TITLE: Extraction Procedure
AUTHORS: Jing Xu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Dried leaves of </span><span style = "font-style:italic;">C. cyrtophyllum</span><span> (150 g) were weighted and sieved (20 mesh) in an herb disintegrator (118 Swing, Zhejiang, China); the powdered sam... | [] |
69,604 | Evaluation of seed morphology, seedling genetic variation, and components for seed storage of Agave landraces of commercial interest. | 3 | dx.doi.org/10.17504/protocols.io.6qpvr6j12vmk/v1 | https://www.protocols.io/view/evaluation-of-seed-morphology-seedling-genetic-var-cf8ctrsw | Jesus A Jimenez-Torres, Zurisadai Monroy-Gonzalez, Juana Juarez-Muñoz | TITLE: Evaluation of seed morphology, seedling genetic variation, and components for seed storage of Agave landraces of commercial interest.
AUTHORS: Jesus A Jimenez-Torres, Zurisadai Monroy-Gonzalez, Juana Juarez-Muñoz
[DESCRIPTION]
Sexual propagation of Agave plants is an incipient cultivation method, these plants ... | [] |
51,100 | Standard PCR protocol | 4 | null | https://www.protocols.io/view/standard-pcr-protocol-bv54n88w | Victoria Jackson | TITLE: Standard PCR protocol
AUTHORS: Victoria Jackson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Generic PCR protocol</div></div>
[STEPS]
?. [Master mix]
For a reaction volume use the following recipe: dH2O buffer MgCl2 FWD primer (from a working solution) REV primer (from a working solu... | ["[Master mix]\nFor a reaction volume use the following recipe: dH2O buffer MgCl2 FWD primer (from a working solution) REV primer (from a working solution) dNTPs (from a mix) Taq polymerase\n25 µl\n14.9 µl\n5 µl\n1.5 µl\n1 µl\n1 µl\n0.5 µl\n0.1 µl", "[Master mix]\nPrepare the master mix allowing for 10% pipett... |
50,018 | PiggyBac Transfection iPSC | 1 | dx.doi.org/10.17504/protocols.io.bu4anyse | https://www.protocols.io/view/piggybac-transfection-ipsc-bu4anyse | Aazam Vahdatshoar | TITLE: PiggyBac Transfection iPSC
AUTHORS: Aazam Vahdatshoar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details the generation of stably transfected iPSC with piggybac constructs. This procedure is used to introduce exogenous NGN2 gene into iPSCs. The established cell lines are us... | ["[Day before transfection: Re-plating the cells]\nAspirate the medium from one confluent well of 6-well plate, and wash once with PBS.", "[Day before transfection: Re-plating the cells]\nAdd accutase and leave in the incubator for .\n1 mL", "[Day before transfection: Re-plating the cells]\nAdd growth medium or hES m... |
73,478 | ProExM | 4 | null | https://www.protocols.io/view/proexm-cjzeup3e | monica.fernandez-monreal | TITLE: ProExM
AUTHORS: monica.fernandez-monreal
[DESCRIPTION]
Classical protocol of protein pre-labeling Expansion- ProExM
(c) Ed Boyden
[STEPS]
SECTION: Solutions
1. Gel and disgestion
SECTION: Solutions
2. Fixation
SECTION: Fluorescence Labeling
3. Fixation: Use fresh 4% PFA solution in PBS (w/ or wo/ 4% sucrose) f... | ["[Solutions] Gel and disgestion", "[Solutions] Fixation", "[Fluorescence Labeling] Fixation: Use fresh 4% PFA solution in PBS (w/ or wo/ 4% sucrose) for 15-20 min. Wash 3 times in PBS and quench aldehydes with glycine or sodium borate.", "[Fluorescence Labeling] Permeabilize in blocking buffer (0.2% fish gelatin, 0.1%... |
70,631 | Data Processing of Technologica Chlorophyll Fluorescence Imager Data for Photoprotection and NPQ Relaxation | 5 | null | https://www.protocols.io/view/data-processing-of-technologica-chlorophyll-fluore-cg8ftztn | Lynn Doran | TITLE: Data Processing of Technologica Chlorophyll Fluorescence Imager Data for Photoprotection and NPQ Relaxation
AUTHORS: Lynn Doran
[DESCRIPTION]
Data Processing of Technologica Chlorophyll Fluorescence Imager Data for Photoprotection and NPQ Relaxation using Matlab and Microsoft Excel to generate graphs of NPQ o... | ["Open the .igr file generated from the CF Imager using the FluorImager software.", "If you are using a computer removed from the instrumentation for data processing, click OK and ignore the warning about camera calibration. The camera is not necessary for data processing.", "If initial screen that comes up, has \"Aut... |
42,231 | MAIT Cell Adoptive Transfer | 4 | dx.doi.org/10.17504/protocols.io.bmgxk3xn | https://www.protocols.io/view/mait-cell-adoptive-transfer-bmgxk3xn | Timothy S C Hinks, Bonnie van Wilgenburg, Huimeng Wang, Liyen Loh, Marios Koutsakos, Katherine Kedzierska, Alexandra J. Corbett, Zhenjun Chen | TITLE: MAIT Cell Adoptive Transfer
AUTHORS: Timothy S C Hinks, Bonnie van Wilgenburg, Huimeng Wang, Liyen Loh, Marios Koutsakos, Katherine Kedzierska, Alexandra J. Corbett, Zhenjun Chen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is part 3.2 of the "Study of MAIT Cell Activation in Viral In... | ["7 days or more after intranasal infection with S. Typhimurium, MAIT cells can be harvested (see Note 9).\nAs MAIT cells are to be used for adoptive transfer, all procedures should be performed in a BSCII biosafety cabinet. All tools and reagents should be sterile.", "Prewarm collagenase media and shaking incubator to... |
68,089 | Proteoform Identification and Quantitation with TopPIC and TDPortal for Human Tissues | 5 | dx.doi.org/10.17504/protocols.io.3byl4bpj2vo5/v1 | https://www.protocols.io/view/proteoform-identification-and-quantitation-with-to-ceqztdx6 | James M Fulcher, Yen-Chen Liao, Mowei Zhou, Ljiljana.PasaTolic | TITLE: Proteoform Identification and Quantitation with TopPIC and TDPortal for Human Tissues
AUTHORS: James M Fulcher, Yen-Chen Liao, Mowei Zhou, Ljiljana.PasaTolic
[DESCRIPTION]
This protocol describe a workflow for top-down proteomics analysis. Top-down proteomics data are processed with two separate software packag... | ["[TopPIC Processing] Convert Instrument raw data to mzML using MSConvert", "[TopPIC Processing] Analyze mzML files using the TopPIC Suite (version 1.4.13.1) .", "[TopPIC Processing] TopPICR is used for post-processing to improve proteoform identification and quantification. All functions are documented within the TopP... |
23,715 | Open Vegetation Survey Protocol | null | dx.doi.org/10.17504/protocols.io.3ebgjan | null | Sabine St-Jean, Mark Vellend | TITLE: Open Vegetation Survey Protocol
AUTHORS: Sabine St-Jean, Mark Vellend
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Here we describe the standardised protocol used by the </span><a href="http://www.caboscience.org/" style = "text-decoration:underline;color:blue;cursor:pointer;"><span ... | ["[Fieldwork Preparation]\nConfirm with the local project leader (see Guidelines → Site Specific Information) that the plots have been marked in the field and created in Fulcrum. If the plots have already been created in Fulcrum, skip to step 3. If not, go to step 2.", "[Fieldwork Preparation]\nIn Fulcrum, enter contex... |
69,975 | Lysosome proteolysis analysis with DQ-BSA | 4 | dx.doi.org/10.17504/protocols.io.14egn2xmyg5d/v1 | https://www.protocols.io/view/lysosome-proteolysis-analysis-with-dq-bsa-cgjxtupn | gustavo.parfitt | TITLE: Lysosome proteolysis analysis with DQ-BSA
AUTHORS: gustavo.parfitt
[DESCRIPTION]
Protocol to analyze lysosomal proteolysis in astrocytes generated from iPSCs.
[STEPS]
1. Coat a 96 well plate Greiner 96 well Microplates Black with gelatin 0.1% or Geltrex LDEV Free hESC Quality 5 ml Thermo Fisher Scientific Cata... | ["Coat a 96 well plate Greiner 96 well Microplates Black with gelatin 0.1% or Geltrex LDEV Free hESC Quality 5 ml Thermo Fisher Scientific Catalog #A1413302 1:100", "DIssociate extracted astrocytes with for 5 min at 25 °C", "300 x g, 3 min, 25 °C", "Resuspend the pellet in 1 mL of astrocyte media (https://www.scien... |
76,335 | Immunohistochemistry (neural organoids) | 1 | dx.doi.org/10.17504/protocols.io.n92ldp22nl5b/v1 | https://www.protocols.io/view/immunohistochemistry-neural-organoids-cnspvedn | anita.adami | TITLE: Immunohistochemistry (neural organoids)
AUTHORS: anita.adami
[DESCRIPTION]
This protocols describes how to perform immunohistochemistry on neural organoids
[STEPS]
SECTION: Fixing and mounting
1. The organoids were fixed in 4% paraformaldehyde for 120 min at Room temperature .
SECTION: Fixing and mounting
2.... | ["[Fixing and mounting] The organoids were fixed in 4% paraformaldehyde for 120 min at Room temperature .", "[Fixing and mounting] They were subsequently washed three times with KPBS and left in a 1:1 OCT:30% sucrose solution and OCT (HistoLab) mixture 120 min.", "[Fixing and mounting] The organoids were then transferr... |
40,367 | Indirect ELISA for detection of anti-HIV antibodies in cats immunized with a HIV gp120 peptide. | 4 | dx.doi.org/10.17504/protocols.io.bjnpkmdn | https://www.protocols.io/view/indirect-elisa-for-detection-of-anti-hiv-antibodie-bjnpkmdn | Angel Justiz-Vaillant | TITLE: Indirect ELISA for detection of anti-HIV antibodies in cats immunized with a HIV gp120 peptide.
AUTHORS: Angel Justiz-Vaillant
[STEPS]
?. The ELISA describes detection of antibodies against HIV proteins. It is an ELISA with a modification. The plate is coated with HIV peptides, fragment 254-274 of gp120.
?. Th... | ["The ELISA describes detection of antibodies against HIV proteins. It is an ELISA with a modification. The plate is coated with HIV peptides, fragment 254-274 of gp120.", "The modification is that triplicates of 1/16 dilutions of cat sera in PBS non-fat milk are added to the micro-wells.", "After incubation for 90 min... |
93,044 | Delay Discounting Measured Using an Adjusting Amount Procedure | 1 | dx.doi.org/10.17504/protocols.io.kqdg3xn7zg25/v1 | https://www.protocols.io/view/delay-discounting-measured-using-an-adjusting-amou-c64uzgww | Montana Kay Lara, Suzanne H. Mitchell | TITLE: Delay Discounting Measured Using an Adjusting Amount Procedure
AUTHORS: Montana Kay Lara, Suzanne H. Mitchell
[DESCRIPTION]
Delay discounting is the process by which outcomes (rewards or punishment) are devalued as a function of the delay to their occurrence. The adjusting amount procedure enables researchers ... | ["[Training Phase 1 (programs: DDPhase1-withVT and DDPhase1-noVT)] Rats learn to press either lever to obtain sucrose rewards and learn that the feeder wells below the levers are sources of sucrose.", "[Training Phase 1 (programs: DDPhase1-withVT and DDPhase1-noVT)] Contingency 1: Stimulus lights above each lever are l... |
78,987 | Detection of Influenza A viruses and Avian H5 Subtype using a triplex qRT-PCR assay on the ABI Quantstudio 7 PCR system | 4 | dx.doi.org/10.17504/protocols.io.n2bvj81xxgk5/v1 | https://www.protocols.io/view/detection-of-influenza-a-viruses-and-avian-h5-subt-crdjv24n | Tracy Lee, frankie.tsang, Kathleen Kolehmainen, Natalie Prystajecky, Agatha N. Jassem, John R. Tyson, Michael Chan | TITLE: Detection of Influenza A viruses and Avian H5 Subtype using a triplex qRT-PCR assay on the ABI Quantstudio 7 PCR system
AUTHORS: Tracy Lee, frankie.tsang, Kathleen Kolehmainen, Natalie Prystajecky, Agatha N. Jassem, John R. Tyson, Michael Chan
[DESCRIPTION]
This procedure provides instructions on how to perform... | ["[Preparation of 20x Primer/Probe Mix] In the reagent preparation clean room, take out the primers and probes listed in table 1 from the freezer and thaw in the refrigerator.", "[Preparation of 20x Primer/Probe Mix] Once thawed, vortex well and centrifuge down all components", "[Preparation of 20x Primer/Probe Mix] In... |
null | null | null | dx.doi.org/10.17504/protocols.io.gt3bwqn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Recipe for TSS buffer. Buffer can be used for TSS transformation of non-competent<em> E. coli</em> cells.</p>
[STEPS]
?.
?.
?.
?. | [] |
72,207 | SVF Isolation and Immunophenotyping using Flow Cytometry in Leprosy Patients | 1 | dx.doi.org/10.17504/protocols.io.q26g7yym9gwz/v1 | https://www.protocols.io/view/svf-isolation-and-immunophenotyping-using-flow-cyt-cirpud5n | Sondang P. Sirait, Kusmarinah Bramono, Sri Linuwih Menaldi, Jeanne Adiwinata Pawitan, Wresti Indriatmi, Tiara Aninditha | TITLE: SVF Isolation and Immunophenotyping using Flow Cytometry in Leprosy Patients
AUTHORS: Sondang P. Sirait, Kusmarinah Bramono, Sri Linuwih Menaldi, Jeanne Adiwinata Pawitan, Wresti Indriatmi, Tiara Aninditha
[DESCRIPTION]
Adipose derived stromal vascular fraction (SVF) contains a heterogeneous population of monon... | ["[Isolation of Abdominal Adipose Tissue] The area was marked by using skin marker. After aseptic and antiseptic procedures the area was covered with a sterile surgical drape.", "[Isolation of Abdominal Adipose Tissue] The following procedure is performed after patient identification and obtaining patient consent.", "[... |
null | null | null | dx.doi.org/10.17504/protocols.io.dn55g5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is for purification of Cas9::NLS<sub>SV40</sub>::His<sub>6 </sub>from:<br /><span class="cit-title"><span class="cit-auth cit-auth-type-author">Paix A</span><span class="cit-sep cit-sep-separator">,</span><span class="cit-auth cit-auth-type-author"> Folkmann A</spa... | [] |
67,820 | Assessing protein purity using SDS PAGE | 4 | null | https://www.protocols.io/view/assessing-protein-purity-using-sds-page-cegktbuw | Shalo Minette, Stephane Fadanka, Nadine Mowoh | TITLE: Assessing protein purity using SDS PAGE
AUTHORS: Shalo Minette, Stephane Fadanka, Nadine Mowoh
[DESCRIPTION]
SDS-PAGE (Sodium Dodecyl Sulphate polyacrylamide gel electrophoresis) is an electrophoresis method that allows protein separation by mass. The gel acts as a sieve through which the proteins... | ["[Preparing reagent working stocks] Running Buffer Preparation\n \n\nFor 1 L of 1x Tris-MOPS buffer\n\nMeasure out 50 mL of 20X Tris-MOPS buffer into a 1L capacity beaker and add 950 mL of distilled water.\n\nTruPAGE Tris-MOPS SDS Express Running buffers are supplied as 20X concentrated solutions. For best results, it... |
50,814 | Cryosectioning mouse brain | 1 | dx.doi.org/10.17504/protocols.io.e6nvw54jdvmk/v1 | https://www.protocols.io/view/cryosectioning-mouse-brain-bvu6n6ze | Pranay Srivastava, Waijiao Cai, Xiqun Chen | TITLE: Cryosectioning mouse brain
AUTHORS: Pranay Srivastava, Waijiao Cai, Xiqun Chen
[DESCRIPTION]
This protocol details the cryosectioning of mouse brain.
[STEPS]
SECTION: Cryosectioning mouse brain
1. Place the mouse brain in the mouse brain slicer (on ice). Cut the hinder of the brain and put it in 4% PFA at 4... | ["[Cryosectioning mouse brain] Place the mouse brain in the mouse brain slicer (on ice). Cut the hinder of the brain and put it in 4% PFA at 4 °C.", "[Cryosectioning mouse brain] Change the solution to 30% sucrose/PBS at 4 °C until the brain sinks in the bottom ( ).", "[Cryosectioning mouse brain] Change the solution... |
null | null | null | dx.doi.org/10.17504/protocols.io.mygc7tw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.e7kbhkw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is a collection of G-Biosciences MegaLong™ protocols for the isolation of >100kb Genomic DNA .</p>
<p> </p>
<p>MegaLong™ isolates high molecular weight (>100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial ... | [] |
44,774 | Making low peptone NGM for imaging plates | 1 | dx.doi.org/10.17504/protocols.io.bpyempte | https://www.protocols.io/view/making-low-peptone-ngm-for-imaging-plates-bpyempte | Ida Barlow, Priota Islam | TITLE: Making low peptone NGM for imaging plates
AUTHORS: Ida Barlow, Priota Islam
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">C. elegans is maintained in the laboratory on Nematode Growth Medium (NGM) agar which has been aseptically poured into petri plates. The NGM agar medium can be poured in... | ["[Pre-Autoclave:]\nBook the autoclave (notebook on top of the machine). Take clean flasks from the glass kitchen. Measure all the pre-autoclave reagents and add to the flask (Use a new weighing boat and spatula for each reagent. Also, the cholesterol is kept in the fridge.) Once water is added mix thoroughly and label... |
64,452 | Holen Sie das Beste aus Ihrem DIAETOXIL KAPSELN heraus? | 1 | dx.doi.org/10.17504/protocols.io.5jyl89qy6v2w/v1 | https://www.protocols.io/view/holen-sie-das-beste-aus-ihrem-diaetoxil-kapseln-he-ca7cshiw | winsballors | TITLE: Holen Sie das Beste aus Ihrem DIAETOXIL KAPSELN heraus?
AUTHORS: winsballors
[DESCRIPTION]
Click Here To Visit - "OFFICIAL WEBSITE"!
Gewichtsverlust ist für viele Menschen ein häufiges Gesundheitsproblem. Viele Menschen haben begonnen, ins Fitnessstudio zu gehen, um Gewicht und Form zu verlieren. Wellness ist... | ["[Holen Sie das Beste aus Ihrem DIAETOXIL KAPSELN heraus?]"] |
null | null | null | dx.doi.org/10.17504/protocols.io.d7i9kd | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p dir="ltr" style="line-height: 1.3800000000000001; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">Scott, JA, WG brogdon, and FH Coll... | [] |
50,889 | SEGA protocol collection | 2 | dx.doi.org/10.17504/protocols.io.bvxhn7j6 | https://www.protocols.io/view/sega-protocol-collection-bvxhn7j6 | Carolyn Bayer, Maja Rennig, Anja Ehrmann, Morten Norholm | TITLE: SEGA protocol collection
AUTHORS: Carolyn Bayer, Maja Rennig, Anja Ehrmann, Morten Norholm
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">SEGA, the Standardized Genome Engineering Architecture, is a comprehensive strain collection that enables genome engineering by combining only two reage... | [] |
78,845 | Standard Operating Procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates | 4 | dx.doi.org/10.17504/protocols.io.ewov1o1polr2/v1 | https://www.protocols.io/view/standard-operating-procedure-sop-for-systemic-admi-cq85vzy6 | Thomas Wichmann, Adriana Galvan | TITLE: Standard Operating Procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates
AUTHORS: Thomas Wichmann, Adriana Galvan
[DESCRIPTION]
This protocol details standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tet... | ["[Receipt of MPTP] Place the package delivered to the research lab into a chemical fume hood dedicated to MPTP solution preparation.", "[Receipt of MPTP] Don a lab coat and two pairs of nitrile gloves and inspect the packaging to ensure no damage.", "[Receipt of MPTP] Open the box and visually inspect the bottle.", "[... |
29,045 | FFPE Tissue Sectioning for Staining | null | dx.doi.org/10.17504/protocols.io.8kvhuw6 | null | Marda Jorgensen | TITLE: FFPE Tissue Sectioning for Staining
AUTHORS: Marda Jorgensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Method for sectioning paraffin blocks for staining </div></div>
[STEPS]
?. Prepare a water bath at 42°C and place it next to the Microtome.
?. Insert a new blade for sectioning each b... | ["Prepare a water bath at 42°C and place it next to the Microtome.", "Insert a new blade for sectioning each block", "Section the tissue between 4-5 µm thick.", "Place the sectioned tissue in the water bath for a few seconds and observe it expanding.", "When the observed expansion is enough to remove folds and wrinkles... |
24,871 | Making and applying fertilisers in solution form in pot experiments | null | dx.doi.org/10.17504/protocols.io.4ifgubn | null | Matema LE. Imakumbili | TITLE: Making and applying fertilisers in solution form in pot experiments
AUTHORS: Matema LE. Imakumbili
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"><span>This protocol describes how fertiliser solutions can be made and applied to already establi... | ["Determining the amount of fertiliser to add to the soil in each potField based fertiliser rates (in kg/ha) are often used and converted to pot based rates (in g/kg or mg/kg of soil) in pot experiments. We shall use an example to demonstrate this. Our example involves the application of nitrogen (N) at a field based r... |
90,844 | OT-2 PCR sample preparation protocol | 5 | dx.doi.org/10.17504/protocols.io.n92ldpyznl5b/v2 | https://www.protocols.io/view/ot-2-pcr-sample-preparation-protocol-c4x4yxqw | Ana Mariya Anhel, Lorea Alejaldre, Manuel Gimenez, Ángel Goñi-Moreno | TITLE: OT-2 PCR sample preparation protocol
AUTHORS: Ana Mariya Anhel, Lorea Alejaldre, Manuel Gimenez, Ángel Goñi-Moreno
[DESCRIPTION]
This protocol is meant to perform samples preparation of PCR plates for 1 or more primer sets (defined number of primers per set established by the user) to the same samples, i.e, w... | ["[Files Preparation] Preparing Customized Template\n\nPreparing the template (a .xlsx) with the specific variables for each experiment.\n\nHere we attach one Excel with several sheets and a PDF file explaining each variable:\n\nGeneralVariables: variables related mainly to the labware that is going to be used\nSamples... |
99,496 | Study protocol for evaluation of the safety and efficacy of 99mTc-3PRGD2 SPECT/CT for integrin αVβ3-targeted imaging of lung cancer and the lymph node metastases: a prospective, multicenter, self-controlled phase 3 clinical trial | 0 | null | https://www.protocols.io/view/study-protocol-for-evaluation-of-the-safety-and-ef-ddeg23bw | Zhaohui Zhu, Fan Wang | TITLE: Study protocol for evaluation of the safety and efficacy of 99mTc-3PRGD2 SPECT/CT for integrin αVβ3-targeted imaging of lung cancer and the lymph node metastases: a prospective, multicenter, self-controlled phase 3 clinical trial
AUTHORS: Zhaohui Zhu, Fan Wang
[DESCRIPTION]
This is the study protocol for evalua... | ["[Study protocol] Introduction\n1.1 Background\nLung cancer affects 2.5 million new cases and leads to 1.8 million deaths each year around the world, ranking the most frequently diagnosed cancer and also the leading cause of cancer death of all cancers globally. With an increasing number of patients being diagnosed at... |
62,497 | Topographical Organization, Morphology, and Density Analysis of Substance P (SP)-IR axons in the Whole Mouse Stomach | 4 | dx.doi.org/10.17504/protocols.io.j8nlkknn5l5r/v1 | https://www.protocols.io/view/topographical-organization-morphology-and-density-b899rz96 | Duyen Nguyen, Anas Mistareehi, Jichao Ma, Jazune Madas, Andrew M. Kwiat, Kohlton Bendowski, Jin Chen, De-Pei Li, John Furness, Terry Powley, Zixi Jack Cheng | TITLE: Topographical Organization, Morphology, and Density Analysis of Substance P (SP)-IR axons in the Whole Mouse Stomach
AUTHORS: Duyen Nguyen, Anas Mistareehi, Jichao Ma, Jazune Madas, Andrew M. Kwiat, Kohlton Bendowski, Jin Chen, De-Pei Li, John Furness, Terry Powley, Zixi Jack Cheng
[DESCRIPTION]
Thi... | ["[Animals] Male (age range: 12-16 weeks) were used. All animals were housed in a room in which light/dark cycles were set to 12h/12h (6:00 AM to 6:00 PM light cycle) and provided food and water ad libitum. \n\nAll procedures were approved by the University of Central Florida Animal Care and Use Committee and strictl... |
48,005 | APPearl Protocolo v1.0 | 3 | null | https://www.protocols.io/view/appearl-protocolo-v1-0-bs5dng26 | José de Jesús Vidal-Mayo, Thierry Hernández Gilsoul, Javier Mancilla-Galindo, Ashuin Kammar-Garcia, Ramón Alejandro Loeza Güemez, Nielzer Armando Rodríguez Almendros, Omar Yaxmehen Bello-Chavolla | TITLE: APPearl Protocolo v1.0
AUTHORS: José de Jesús Vidal-Mayo, Thierry Hernández Gilsoul, Javier Mancilla-Galindo, Ashuin Kammar-Garcia, Ramón Alejandro Loeza Güemez, Nielzer Armando Rodríguez Almendros, Omar Yaxmehen Bello-Chavolla
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.sp5edq6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
A protocol for extracting RNA from Aiptasia, modified from the Weis lab protocol by Angela Poole and Mauricio Rodriguez-Lanetty. Note that the final product has significant DNA contamination and needs to be cleaned up via DNase treatment.
[BEFORE_START]
<p>Make sure to setup... | ["{\"blocks\":[{\"key\":\"30itn\",\"text\":\"Homogenize starting material in Trizol using a micropestle. Trizol volume should be ~ 1 mL per 0.1g tissue. Once the homogenate is uniform, incubate at room temperature for 5 minutes. \",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":... |
35,311 | Cryogrinding protocol : mecanic lysis of planktonic filter for RNA/DNA extraction | 1 | dx.doi.org/10.17504/protocols.io.beqpjdvn | https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-beqpjdvn | Sarah Romac | TITLE: Cryogrinding protocol : mecanic lysis of planktonic filter for RNA/DNA extraction
AUTHORS: Sarah Romac
[DESCRIPTION]
Cell Lysis can be difficult to conduct for envirronmental water samples collected on big filters, specially when big water volumes (20-100L) have been filtered on polycarbanate 142mm-diameter f... | ["[FreezerMill Tube Assembly] Finish the assembly of the cryogrinding tube : insert the magnet inside the tube and close the tube with the other extremity containing the hole.", "[FreezerMill Tube Assembly] Put the tube in the FreezerMill chamber, and check the liquid nitrogen level and close the FreezerMill.", "[Freez... |
98,386 | Example | 0 | null | https://www.protocols.io/view/example-dcbs2sne | Antony Borel | TITLE: Example
AUTHORS: Antony Borel
[DESCRIPTION]
A
[STEPS]
1.
2.
3.
1.1.
1.2. | [] |
38,188 | SEM User Guide (Hubbs Hall) | 1 | dx.doi.org/10.17504/protocols.io.5qpvo54kbl4o/v1 | https://www.protocols.io/view/sem-user-guide-hubbs-hall-bhikj4cw | Greg Rouse | TITLE: SEM User Guide (Hubbs Hall)
AUTHORS: Greg Rouse
[DESCRIPTION]
Basics on how to use the SEM machine at Hubbs Hall at SIO.
[GUIDELINES]
Contact Greg Rouse or Martin Tresguerres with any questions.
[STEPS]
SECTION: Power On/Set-up
1. The machine will be either shut down (red button) or standby (yellow button). I... | ["[Power On/Set-up] The machine will be either shut down (red button) or standby (yellow button). If red press the yellow button and wait a few minutes. Then press green button (ON) - this turns on the computer, and activates the vacuum pump and powers the SEM.", "[Power On/Set-up] From the PC desktop, double click on ... |
95,202 | An efficient and cost-effective method for disrupting genes in RAW264.7 macrophages using CRISPR-Cas9 | 4 | dx.doi.org/10.17504/protocols.io.rm7vzxzxrgx1/v1 | https://www.protocols.io/view/an-efficient-and-cost-effective-method-for-disrupt-c88azzse | Mohammed J. Hossain, Tamara J. O'Connor | TITLE: An efficient and cost-effective method for disrupting genes in RAW264.7 macrophages using CRISPR-Cas9
AUTHORS: Mohammed J. Hossain, Tamara J. O'Connor
[DESCRIPTION]
CRISPR-Cas9 genome editing is widely used to modify cultured cell lines. However, its implementation is still challenging due to the complex and of... | ["A. Design guide RNAs", "Predict sgRNA(s) using the GPP Web Portal: https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design", "Under the ‘CRISPRko’ tab,", "a) In the “CRISPR Enzyme” drop-down menu, select ‘SpyoCas9 (NGG)’.", "b) In the “Target Genome” drop-down menu, select ‘Mouse GRCm38 (... |
42,824 | MPAPASS Software Collection | 2 | dx.doi.org/10.17504/protocols.io.bm3gk8jw | https://www.protocols.io/view/mpapass-software-collection-bm3gk8jw | Joshua Welsh, Sean Cook, Jennifer Jones | TITLE: MPAPASS Software Collection
AUTHORS: Joshua Welsh, Sean Cook, Jennifer Jones
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is collection contains the protocols required for each step in the mpapass software pipeline for performing stitched multiplex analysis. This is one of a number of... | [] |
92,327 | Ea(52-68) uptake assays | 4 | dx.doi.org/10.17504/protocols.io.14egn3r3pl5d/v1 | https://www.protocols.io/view/ea-52-68-uptake-assays-c6efzbbn | Rebecca Wallings | TITLE: Ea(52-68) uptake assays
AUTHORS: Rebecca Wallings
[DESCRIPTION]
Ea(52-68) uptake assays on pMacs
[STEPS]
1. Reconstitute MHC-II Ea chain (Ea) (52–68) peptide (Anaspec) in sterile distilled H2O to a final concentrate of 1mg/mL.
Once peritoneal macrophages are adhered to plates, add 5µg per well of 24-well pla... | ["Reconstitute MHC-II Ea chain (Ea) (52–68) peptide (Anaspec) in sterile distilled H2O to a final concentrate of 1mg/mL. \n\nOnce peritoneal macrophages are adhered to plates, add 5µg per well of 24-well plate and incubate for 18-hours\n\nHarvest cells as per flow cytometry protocol and stain with eBioY-Ae antibody (T... |
70,065 | BARseq - high-throughput cell typing with in situ sequencing | 1 | dx.doi.org/10.17504/protocols.io.81wgbp4j3vpk/v2 | https://www.protocols.io/view/barseq-high-throughput-cell-typing-with-in-situ-se-cgnrtvd6 | Xiaoyin Chen, Anthony M. Zador, Mararue | TITLE: BARseq - high-throughput cell typing with in situ sequencing
AUTHORS: Xiaoyin Chen, Anthony M. Zador, Mararue
[DESCRIPTION]
This protocol describes the application of BARseq as a standalone in situ sequencing method to achieve multiplexed interrogation of endogenous genes. In this variation, BARseq is similar ... | ["[Library preparation] Tissues with barcoded neurons should be cryo-sectioned to 20 μm and mounted on slides. Slides can be stored at -80 °C for up to a month.", "[Library preparation] DAY 1\n\nTake slide(s) out of -80 °C and immerse immediately in 4% paraformaldehyde in 1x PBS (2 slides per 50mL falcon tube, back-to-... |
63,940 | Nucleotide Synthesis Services | 6 | dx.doi.org/10.17504/protocols.io.5qpvobwxbl4o/v1 | https://www.protocols.io/view/nucleotide-synthesis-services-capcsdiw | BOC Sciences | TITLE: Nucleotide Synthesis Services
AUTHORS: BOC Sciences
[DESCRIPTION]
BOC Sciences has years of professional experience in providing nucleotide synthesis services for customers all over the world. Based on its sophisticated equipment, advanced technologies, and highly experienced staff, it can provide you a f... | [] |
104,381 | Sinai SCENT TMC- Methylation Array (EPIC) | 0 | dx.doi.org/10.17504/protocols.io.81wgbzqyygpk/v1 | https://www.protocols.io/view/sinai-scent-tmc-methylation-array-epic-dh6539g6 | Sojin Kim | TITLE: Sinai SCENT TMC- Methylation Array (EPIC)
AUTHORS: Sojin Kim
[DESCRIPTION]
High throughput, proven procedure for bisulfite conversion of DNA
96-well desulphonation and recovery of bisulfite-treated DNA
[STEPS]
SECTION: High-Throughput Bisulfite Conversion of DNA
1. Add 5 μl of M-Dilution Buffer to each DNA sam... | ["[High-Throughput Bisulfite Conversion of DNA] Add 5 μl of M-Dilution Buffer to each DNA sample in a Conversion Plate and adjust the total volume to 50 μl with water. Mix each sample by pipetting up and down.\n\nExample: For 14 μl of a DNA sample add 5 μl M-Dilution Buffer and 31 μl water.", "[High-Throughput Bisulfit... |
null | null | null | dx.doi.org/10.17504/protocols.io.esgbebw | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<p><strong>Reagents</strong></p>
<p>Liquid (88%) phenol</p>
<p>1% w/w Ficoll 400</p>
<p>0.5M DTT solution (77 mg/ml)</p>
<p>10M LiCl</p>
<p>10% w/v SDS</p>
<p>1% Ponceau S (dye)</p>
<p>Acetone<br /><br /><strong>Purifying proteins<br /></strong>Proteins are easily precipitated fr... | [] |
90,292 | Consideration of the information needs of pregnant women with elective caesarean section in antenatal classes – a qualitative study from the perspective of midwives | 1 | dx.doi.org/10.17504/protocols.io.kqdg3x7dpg25/v1 | https://www.protocols.io/view/consideration-of-the-information-needs-of-pregnant-c4euytew | Sandra Jaax, Barbara Prediger, Nadja Könsgen | TITLE: Consideration of the information needs of pregnant women with elective caesarean section in antenatal classes – a qualitative study from the perspective of midwives
AUTHORS: Sandra Jaax, Barbara Prediger, Nadja Könsgen
[DESCRIPTION]
Background: Qualitative studies show that pregnant women often feel inadequate... | [] |
63,922 | Carbohydrate Services | 6 | dx.doi.org/10.17504/protocols.io.x54v9yz91g3e/v1 | https://www.protocols.io/view/carbohydrate-services-canssdee | BOC Sciences | TITLE: Carbohydrate Services
AUTHORS: BOC Sciences
[DESCRIPTION]
BOC Sciences supports the research and development of carbohydrate synthesis with an established international reputation. BOC Sciences' advanced capabilities in carbohydrate synthesis, purification and identification have benefited our customers i... | [] |
96,457 | Cost-reducing Nanopore Library Preparation for R10 Native Barcoding Kit | 4 | dx.doi.org/10.17504/protocols.io.81wgbxm51lpk/v1 | https://www.protocols.io/view/cost-reducing-nanopore-library-preparation-for-r10-dafh2bj6 | Guan Jie Phang | TITLE: Cost-reducing Nanopore Library Preparation for R10 Native Barcoding Kit
AUTHORS: Guan Jie Phang
[DESCRIPTION]
Cost-reducing protocols for Nanopore R10 flow cell Native Barcoding library construction.
[STEPS]
SECTION: Step1. End-prep
2. In 200 µL tube(s), combine the following chemicals per tube:
SEC... | ["[Step1. End-prep] In 200 µL tube(s), combine the following chemicals per tube:", "[Step1. End-prep] Incubate in a thermocycler at 20 °C for 30 min and 65 °C for 30 min. Hold at 4 °C", "[Step2. Adapter ligation] Thaw the Native Adapter (LA) and KAPA HyperPrep Ligation Buffer. Vortex and spin down the tubes, and place ... |
59,146 | Isolation of lysosomes using the Tagless LysoIP method in PBMCs | 4 | dx.doi.org/10.17504/protocols.io.x54v9yp51g3e/v1 | https://www.protocols.io/view/isolation-of-lysosomes-using-the-tagless-lysoip-me-b5ziq74e | Daniel Saarela, Esther Sammler, Dario R Alessi, Francesca Tonelli | TITLE: Isolation of lysosomes using the Tagless LysoIP method in PBMCs
AUTHORS: Daniel Saarela, Esther Sammler, Dario R Alessi, Francesca Tonelli
[DESCRIPTION]
Molecular homeostasis in cells is regulated in part by protein degradation, which is facilitated by the proteasome and lysosomal proteolysis. Lysosomes are mem... | ["[Methods] Prepare a 0.5 Molarity (M) stock solution of DIFP by diluting in isopropanol under a fume hood. Stock solution can be stored in -80 °C until needed.", "[Methods] Collect blood into a BD Vacutainer sodium heparin 10-mL tubes.", "[Methods] Add density gradient medium to the SepMate tube using a 20-mL syringe ... |
49,974 | Sequential extraction and immunoblotting | 1 | dx.doi.org/10.17504/protocols.io.bu2wnyfe | https://www.protocols.io/view/sequential-extraction-and-immunoblotting-bu2wnyfe | Isabel Lam | TITLE: Sequential extraction and immunoblotting
AUTHORS: Isabel Lam
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol examines the fraction of alpha-synuclein (as assessed by alpha-synuclein and/or PS129 western blot) that is present in the triton-soluble or SDS-soluble fraction. Additio... | ["[Sequential extraction and immunoblotting]\nRinse the neurons twice with PBS.", "[Sequential extraction and immunoblotting]\nPlace the dish . By working one well at a time, completely aspirate the PBS and add the following volumes of ice-cold 1% (vol/vol) TX-100/TBS with protease and phosphatase inhibitors: per well... |
null | null | null | dx.doi.org/10.17504/protocols.io.k7sczne | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
106,024 | Isolated Brain Mitochondria Respiration protocol | 0 | dx.doi.org/10.17504/protocols.io.bp2l62z4zgqe/v1 | https://www.protocols.io/view/isolated-brain-mitochondria-respiration-protocol-djsg4nbw | Livia Hecke Morais, Linsey Stiles | TITLE: Isolated Brain Mitochondria Respiration protocol
AUTHORS: Livia Hecke Morais, Linsey Stiles
[DESCRIPTION]
Brain Mitochondria Respiration protocol. Assay developed by Dr. Linsey Stiles at the UCLA Metabolomics
Center, David Geffen School of Medicine at the University of California, Los Angeles, CA, USA
[STEPS]
... | ["[Preparation] The day prior to the Seahorse assay, a Seahorse cartridge needs to be hydrated overnight.", "[Load the Seahorse Cartridge] Load 20 µL per port in the Seahorse cartridge with a multichannel pipet", "[Load the Seahorse Cartridge] Prepare the compounds in MAS to be loaded into the Seahorse cartridge ports.... |
52,277 | Protocol for Safe, Affordable, and Reproducible Isolation of SARS-CoV-2 RNA from Wastewater | 4 | dx.doi.org/10.17504/protocols.io.bxavpie6 | https://www.protocols.io/view/protocol-for-safe-affordable-and-reproducible-isol-bxavpie6 | Monica Trujillo, Kristen Cheung, Anna Gao, Irene Hoxie, Sherin Kannoly, Kaung Myat San, Davida S. Smyth, John Dennehy | TITLE: Protocol for Safe, Affordable, and Reproducible Isolation of SARS-CoV-2 RNA from Wastewater
AUTHORS: Monica Trujillo, Kristen Cheung, Anna Gao, Irene Hoxie, Sherin Kannoly, Kaung Myat San, Davida S. Smyth, John Dennehy
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The following proto... | ["[Day 1 - Pasteurization and Solids removal]\nSample handlingThe samples must be kept at 4°C at all times after collection. Keep wastewater sample containers closed throughout the entire procedure except where noted.", "[Day 1 - Pasteurization and Solids removal]\nPasteurizationIn our protocol, samples are pasteurized... |
29,415 | CRISPR RNP Electroporation Protocol | null | dx.doi.org/10.17504/protocols.io.8yfhxtn | null | Aditya Mohan | TITLE: CRISPR RNP Electroporation Protocol
AUTHORS: Aditya Mohan
[STEPS]
?. [Culture Cells]
Subculture cells for a minimum of 2-3 days before electroporation, and visually inspect the cells with a microscope to ensure healthy appearance.
?. [Form the RNP Complex]
For each well-undergoing electroporation, dilute the gu... | ["[Culture Cells]\nSubculture cells for a minimum of 2-3 days before electroporation, and visually inspect the cells with a microscope to ensure healthy appearance.", "[Form the RNP Complex]\nFor each well-undergoing electroporation, dilute the guide RNA and Cas9 enzyme in PBS gently swirling the pipet tip while pipett... |
62,424 | Prima Keto UK challenge For Weight loss | 1 | dx.doi.org/10.17504/protocols.io.bp2l61r8kvqe/v1 | https://www.protocols.io/view/prima-keto-uk-challenge-for-weight-loss-b87yrzpw | health | TITLE: Prima Keto UK challenge For Weight loss
AUTHORS: health
[DESCRIPTION]
Prima Keto UK challenge For Weight loss
[STEPS]
SECTION: Prima Keto UK challenge For Weight loss
1. these medicines may help burn fat for the energy to do strenuous exercise. They may also help you lose weight. If you take these medicines ... | ["[Prima Keto UK challenge For Weight loss] these medicines may help burn fat for the energy to do strenuous exercise. They may also help you lose weight. If you take these medicines daily, you may notice an increase in energy levels. They may also help reduce fatigue and weakness in your body. Taking these tablets may... |
108,761 | AAV-Zombie on cultured cells | 0 | dx.doi.org/10.17504/protocols.io.36wgqnz53gk5/v2 | https://www.protocols.io/view/aav-zombie-on-cultured-cells-dnfz5bp6 | Gerard Michael Coughlin | TITLE: AAV-Zombie on cultured cells
AUTHORS: Gerard Michael Coughlin
[DESCRIPTION]
Detection of AAV genomes in situ can facilitate understanding of AAV transduction and processing. This protocol enables detection of AAV genomes and concatemers in cultured cells and is based on the Zombie method published in Askary et ... | ["[Reagent set up] General note on reagents and consumables", "[Sample preparation] At time of collection, aspirate media and wash cells one time with 1 mL DPBS. Aspirate DPBS and add 1 mL ice-cold MAA fixation buffer. Transfer to -20 °C for 15 min .", "[Sample preparation] Transduce cells with AAVs carrying Zombie ba... |
22,021 | Five things you need to know about PD-1 inhibitor | null | dx.doi.org/10.17504/protocols.io.zrdf526 | null | Alex Brown | TITLE: Five things you need to know about PD-1 inhibitor
AUTHORS: Alex Brown
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><a style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">PD-1 inhibitors</span></a><span>, including PD-1 antibodies and PD-L1 antibodies, are a ne... | [] |
35,867 | RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit | null | dx.doi.org/10.17504/protocols.io.be93jh8n | https://www.protocols.io/view/rna-purification-from-buccal-swabs-nasopharyngeal-be93jh8n | New England Biolabs | TITLE: RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol utilizes the Monarch Total RNA Miniprep Kit to purify RNA from buccal s... | ["[Part 1: Sample Disruption and Homogenization]\nSample Disruption and Homogenization", "[Part 2: RNA Binding and Elution]", "[Part 1: Sample Disruption and Homogenization]\nImmediately stabilize after collection by mixing with an equal volume of 2X Monarch DNA/RNA Protection Reagent.\n[of the saliva sample]\nIf unab... |
70,611 | 城市微生物相調查計畫—採集土壤教學 | 1 | dx.doi.org/10.17504/protocols.io.n92ldpyj9l5b/v1 | https://www.protocols.io/view/protocol-cg7ttznn | Hsin-Mao Wu | TITLE: 城市微生物相調查計畫—採集土壤教學
AUTHORS: Hsin-Mao Wu
[DESCRIPTION]
吳昕懋
[STEPS]
SECTION: 採土與iNaturalist紀錄教學
1. 移除表土，取距離土表5~7公分之處的土，裝入塑膠夾鏈袋中
SECTION: 採土與iNaturalist紀錄教學
2. 將塑膠袋中的土均勻混合，倒入採集罐之中
SECTION: 採土與iNaturalist紀錄教學
3. 在採集紀錄紙上勾選採樣地點、採樣種類，以及填寫日期、城市、鄉鎮、地點、採集者等資訊
SECTION: 採土與iNaturalist紀錄教學
4. 最後拍兩張照片
... | ["[採土與iNaturalist紀錄教學] 移除表土，取距離土表5~7公分之處的土，裝入塑膠夾鏈袋中", "[採土與iNaturalist紀錄教學] 將塑膠袋中的土均勻混合，倒入採集罐之中", "[採土與iNaturalist紀錄教學] 在採集紀錄紙上勾選採樣地點、採樣種類，以及填寫日期、城市、鄉鎮、地點、採集者等資訊", "[採土與iNaturalist紀錄教學] 最後拍兩張照片", "[採土與iNaturalist紀錄教學] 接著打開iNaturalist app", "[採土與iNaturalist紀錄教學] 如圖所示，點選右下角的加號", "[採土與iNaturalist紀錄教學] 如下圖所示接著需選擇觀察類型，請點「選擇... |
36,049 | Rab29 fast exchange mutants: characterization of a challenging Rab GTPase | null | dx.doi.org/10.17504/protocols.io.bffrjjm6 | https://www.protocols.io/view/rab29-fast-exchange-mutants-characterization-of-a-bffrjjm6 | Rachel C. Gomez, Edmundo G. Vides, Suzanne Pfeffer | TITLE: Rab29 fast exchange mutants: characterization of a challenging Rab GTPase
AUTHORS: Rachel C. Gomez, Edmundo G. Vides, Suzanne Pfeffer
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Rab29 has been implicated in multiple membrane trafficking processes with no described effectors or regulating ... | [] |
76,262 | Assembly: Chronic recoverable Neuropixels in mice | 1 | dx.doi.org/10.17504/protocols.io.eq2lynnewvx9/v7 | https://www.protocols.io/view/assembly-chronic-recoverable-neuropixels-in-mice-cnqevdte | Emily A Aery Jones | TITLE: Assembly: Chronic recoverable Neuropixels in mice
AUTHORS: Emily A Aery Jones
[DESCRIPTION]
This protocol collection explains how to build a low-cost, lightweight system to implant 1 Neuropixels 1.0 probe or 2 Neuropixels 2.0 probes into mice, record during freely moving behavior, then recover the probes for fu... | ["[3D print components] Single 1.0 probe: Build a print file for the following pieces per mouse: 1 each of body piece, back and front flex cable holders, and dome, plus 2 wings. \n\nDual 2.0 probes: Build a print file for the following pieces per mouse: 1 each of left body, right body, flex holder 1, flex holder 2 wit... |
98,423 | Assessment of [18F] C05-05 PET Imaging in Participants with Parkinson's Disease in the PPMI Study (PPMI C05-05 PET Imaging) | 0 | dx.doi.org/10.17504/protocols.io.eq2lyw3ymvx9/v1 | https://www.protocols.io/view/assessment-of-18f-c05-05-pet-imaging-in-participan-dccx2sxn | Kenneth Marek | TITLE: Assessment of [18F] C05-05 PET Imaging in Participants with Parkinson's Disease in the PPMI Study (PPMI C05-05 PET Imaging)
AUTHORS: Kenneth Marek
[DESCRIPTION]
This protocol details the Assessment of [ 18F] C05-05 PET Imaging in Participants with Parkinson's Disease in the PPMI Study (PPMI C05-05 PET Imagi... | ["[PURPOSE OF STUDY] The overall goal of this protocol is to investigate [18F] C05-05 PET imaging, targeting alpha synuclein, in Parkinson’s Disease (PD) participants and healthy volunteers participating in the PPMI study. Participants must meet the following criteria to enroll.", "[STUDY OUTCOMES] The primary study ou... |
65,417 | Standard Operating Procedure: Mouse transcardiac perfusion protocol | 1 | dx.doi.org/10.17504/protocols.io.3byl4b8r8vo5/v1 | https://www.protocols.io/view/standard-operating-procedure-mouse-transcardiac-pe-cb5hsq36 | chu , Hong-Yuan Chu | TITLE: Standard Operating Procedure: Mouse transcardiac perfusion protocol
AUTHORS: chu , Hong-Yuan Chu
[DESCRIPTION]
This protocol details the procedure of the mouse transcardiac perfusion.
[BEFORE_START]
Wear PPEs before entering perfusion room, i.e. gloves, coat, hair net, safety glasses and face mask.
Make sur... | ["[Perfusion Surgery] Make an incision through the abdominal skin.", "[Perfusion Surgery] Make two additional skin incisions from the xiphoid process along the base of the ventral ribcage laterally.", "[Perfusion Surgery] Gently reflect the two flaps of skin to expose thoracic field completely.", "[Perfusion Surgery] G... |
null | null | null | dx.doi.org/10.17504/protocols.io.qradv2e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>From here, You can know detail methods of Hi-C assembly of the Betta splendens genome.</p>
[STEPS]
?.
?.
?. | [] |
42,034 | BSCI:414 Lab2--Create PCR Reaction | 1 | null | https://www.protocols.io/view/bsci-414-lab2-create-pcr-reaction-bmask2ee | Harley King | TITLE: BSCI:414 Lab2--Create PCR Reaction
AUTHORS: Harley King
[STEPS]
?. [Attach Exisiting Primers to Plasmid in Benchling]
Select the primers icon from the right side. Select "Attach Existing". Next click "Add locations". In the popup, select "BSCI:414 Oligos" under the class root folder. Ensure a green checkmark i... | ["[Attach Exisiting Primers to Plasmid in Benchling]\nSelect the primers icon from the right side. Select \"Attach Existing\". Next click \"Add locations\". In the popup, select \"BSCI:414 Oligos\" under the class root folder. Ensure a green checkmark is beside it and that it has been added to the \"Use Primers in\" fi... |
88,191 | Generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line | 4 | dx.doi.org/10.17504/protocols.io.4r3l2268pl1y/v1 | https://www.protocols.io/view/generation-of-a549-ppm1h-bromotag-crispr-cas9-knoc-c2c7yazn | Tran Le Cong Huyen Bao Phan, Thomas Macartney, Dario Alessi | TITLE: Generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line
AUTHORS: Tran Le Cong Huyen Bao Phan, Thomas Macartney, Dario Alessi
[DESCRIPTION]
This protocol details the generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line.
[STEPS]
SECTION: Transfection for PPM1H BromoTag
1.
Seed wild type ... | ["[Transfection for PPM1H BromoTag] Seed wild type A549 cells approximately 60 – 70% confluency in 3 mL media/well (DMEM + 10% FBS (Foetal Bovine Serum) + 1% penicillin/streptomycin + 1% L-glutamine) in 6-well plates. Preferably plate 2 wells per guide + donor DNA (one well for cell growing and one for immunoblotting).... |
68,744 | High resolution labeling of vagal efferent fibers using Dextran-Biotin with counterstaining | 1 | dx.doi.org/10.17504/protocols.io.kxygxmqqwl8j/v2 | https://www.protocols.io/view/high-resolution-labeling-of-vagal-efferent-fibers-cfdgti3w | Deborah Jaffey, Terry Powley, Jennifer Mcadams, Robert Phillips | TITLE: High resolution labeling of vagal efferent fibers using Dextran-Biotin with counterstaining
AUTHORS: Deborah Jaffey, Terry Powley, Jennifer Mcadams, Robert Phillips
[DESCRIPTION]
This protocol describes the methods used to trace and enable morphometric quantification of preganglionic efferent neurites in the ra... | ["[Animals] rats (males: two to four months, mean weight 247g [sd 42g]; females: two to five months, mean weight: 189g [sd 15g] at the time of tracer injection) are housed individually in wire hanging cages or in vented rack plastic cages in an Association for Assessment and Accreditation of Laboratory Animal Care-appr... |
106,311 | Image Capture and Neuropathological Quantification of post-mortem human brain tissue | 0 | dx.doi.org/10.17504/protocols.io.n92ld86qnv5b/v1 | https://www.protocols.io/view/image-capture-and-neuropathological-quantification-dj3f4qjn | Felicia Suteja, Hongyun Li, YuHong Fu, Glenda Halliday | TITLE: Image Capture and Neuropathological Quantification of post-mortem human brain tissue
AUTHORS: Felicia Suteja, Hongyun Li, YuHong Fu, Glenda Halliday
[DESCRIPTION]
This protocol details the fluorescent image analysis pipeline using QuPath to analyse pathological inclusions within human post-mortem brain tissue.
... | ["[Cell quantification] Post-mortem human immunofluorescent sections were scanned using an Olympus VS200 slide scanner at 20x for Quantification. \n\nCell count and area measurements were completed on digital images using QuPath (version 0.5.1). The QuPath quantification protocol followed the steps listed below.", "[An... |
null | null | null | dx.doi.org/10.17504/protocols.io.ggrbtv6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Optical mapping technologies assist the assembly of large and complex genomes. Although protocols for preparing this kind of samples are now conveniently available in kits, some plants won't respond to the standardized conditions or the researcher might not have access to it.... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.h92b98e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
32,724 | Bioflux Analyses: Image Preprocessing | null | dx.doi.org/10.17504/protocols.io.bb7uirnw | null | Tobias Weise, Bettina Boettcher, Slavena Vylkova | TITLE: Bioflux Analyses: Image Preprocessing
AUTHORS: Tobias Weise, Bettina Boettcher, Slavena Vylkova
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Biofilm formation under shear flow conditions was monitored using the Bioflux1000 device (Fluxion Biosciences, Inc.). In short, Candida albicans over... | ["[Video File to Frames]\nConvert video file into single imagesThe source material provided as .AVI file is converted into single .TIFF images using opencv-python. Additional, a data frame containing meta data annotations is created.", "[Video File to Frames]\nImport of the required packages.import numpy as npimport pa... |
54,240 | DRAGEN COVID Lineage App SARS-CoV-2 Strain Characterization on the Illumina BaseSpace Platform | 5 | null | https://www.protocols.io/view/dragen-covid-lineage-app-sars-cov-2-strain-charact-by78pzrw | Technical Outreach and Assistance for States Team | TITLE: DRAGEN COVID Lineage App SARS-CoV-2 Strain Characterization on the Illumina BaseSpace Platform
AUTHORS: Technical Outreach and Assistance for States Team
[DESCRIPTION]
This protocol provides instructions on how to run the DRAGON COVID Lineage app on the Illumina BaseSpace Sequence Hub. The DRAGON COVID Lineage... | ["[Create Basespace Project] Login to the Illumina BaseSpace Platform and create a new BaseSpace project", "[Add Fastq files to BaseSpace Project] Add Fastq files to the new project directory. This can be done by either uploading fastq files from a local directory or by importing sequences using their Sequence Read Arc... |
99,209 | Comparison of Multiple Snap Freezing Protocols and Description of Optimal Workflow | 0 | dx.doi.org/10.17504/protocols.io.81wgbz6yogpk/v1 | https://www.protocols.io/view/comparison-of-multiple-snap-freezing-protocols-and-dc5h2y36 | Zhixin Jing, Andrea Radtke | TITLE: Comparison of Multiple Snap Freezing Protocols and Description of Optimal Workflow
AUTHORS: Zhixin Jing, Andrea Radtke
[DESCRIPTION]
The goal of this protocol is to evaluate the optimal snap (flash) freezing method for multiplexed tissue imaging. We detail four different methods (see sample preparation) for pr... | ["[Sample preparation] Draining and non-draining lymph nodes were dissected and submerged in OCT-filled cryomolds. Orientation of each lymph node was adjusted using a dissection microscope to allow for a sagittal section of the lymph node. Spleen was cut into 2-3 mm thick wedge-shaped pieces with one piece distributed ... |
null | null | null | dx.doi.org/10.17504/protocols.io.rved63e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A very detailed four-day protocol for DNA extraction for yeast PacBio sequencing modified from Promega's protocol for yeast <a href="https://www.promega.co.uk/~/media/files/resources/protocols/technical%20manuals/0/wizard%20genomic%20dna%20purification%20kit%20protocol.pdf" t... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.sjtecnn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>As one of the most recognizable characteristics in birds, plumage colour has a high impact on understanding the evolution and mechanisms of colouration. Feather and skin are ideal tissues to explore the genomics and complexity of colour patterns in vertebrates. Two species of... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.im7cc9n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol allows for adequate DNA extraction from fresh tissue samples.</p>
[BEFORE_START]
<p>Separate PCR-free facility</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
102,176 | ilastik install and run for Syn Bot (Windows Version) | 0 | dx.doi.org/10.17504/protocols.io.5jyl82eedl2w/v1 | https://www.protocols.io/view/ilastik-install-and-run-for-syn-bot-windows-versio-dfz83p9w | Justin T Savage | TITLE: ilastik install and run for Syn Bot (Windows Version)
AUTHORS: Justin T Savage
[DESCRIPTION]
Video instructions for installing ilastik, training an ilastik project, and using the ilastik project for simple SynBot run for Windows operating system.
[STEPS]
1.
1.1. If video quality is poor, try watching di... | ["If video quality is poor, try watching directly from Youtube at https://www.youtube.com/watch?v=ApOwcOFMUcg", "[Installing ilastik] 0:00 - 1:30 Install ilastik by downloading it from ilastik.org/download and running the installer.", "[Generate Training Images] 1:30 - 3:00 Generate training images by running SynBot on... |
44,981 | Primary Culture of Mouse Mesencephalic Neurons | 4 | dx.doi.org/10.17504/protocols.io.bp6vmre6 | https://www.protocols.io/view/primary-culture-of-mouse-mesencephalic-neurons-bp6vmre6 | Nicolas Giguère | TITLE: Primary Culture of Mouse Mesencephalic Neurons
AUTHORS: Nicolas Giguère
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details the procedure required for the dissection and collection of primary mouse culture mesencephalic neurons.</div></div>
[STEPS]
?. [Dissection (preferabl... | ["[Dissection (preferably in pairs)]\nPrepare two containers of crushed ice.", "[Dissection (preferably in pairs)]\nClean the dissection surface with 70% alcohol. Place the dissection tools in a beakers filled with 70% alcohol and a Kimwipe (to protect the tips of the tools).", "[Dissection (preferably in pairs)]\nPrep... |
99,447 | Embedding One Brain For Serial Section Imaging | 0 | null | https://www.protocols.io/view/embedding-one-brain-for-serial-section-imaging-ddcx22xn | Rob Campbell | TITLE: Embedding One Brain For Serial Section Imaging
AUTHORS: Rob Campbell
[DESCRIPTION]
Describes how to embed one perfused mouse brain for serial section microscopy using the SWC's embedding molds.
[BEFORE_START]
A major advantage of serial sectioning is that it generates a seamless 3D data set that can be regist... | ["[Preparing for agar embedding] Place the square (single brain) metal mold on the metal plate as shown. You will use the upper row of holes. Ignore the lower holes: this mold is not used for two brains. The olfactory bulbs will rested on the wire which passes through the indicated hole. This will ensure the ventral br... |
57,957 | LRRK2 RCKW Protein Purification | 4 | dx.doi.org/10.17504/protocols.io.81wgb6693lpk/v1 | https://www.protocols.io/view/lrrk2-rckw-protein-purification-b4udqws6 | David Snead, Yu Xuan Lin, Mariusz Matyszewski | TITLE: LRRK2 RCKW Protein Purification
AUTHORS: David Snead, Yu Xuan Lin, Mariusz Matyszewski
[DESCRIPTION]
Protein purification protocol for tag-less LRRK2RCKW as done by Leschziner and Reck-Peterson Labs. Same protocol can be used to purify LRRK1RCKW as well.
Original Protocol by David Snead. Modified by Yu Xuan L... | ["[Day 1: Protein Pellet Lysis] Ready the Ultracentrifuge and cool it down to 4 °C", "[Day 1: Protein Pellet Lysis] Make Lysis Buffer (check Materials section)", "[Day 1: Protein Pellet Lysis] Thaw protein pellets on ice", "[Day 1: Protein Pellet Lysis] Resuspend each pellet in 40 mL", "[Day 1: Protein Pellet Lysis] H... |
26,283 | Preparing 10 L of M9 buffer for nematode culture | null | dx.doi.org/10.17504/protocols.io.5wjg7cn | null | Gurdon Institute media kitchen | TITLE: Preparing 10 L of M9 buffer for nematode culture
AUTHORS: Gurdon Institute media kitchen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Prepare 10 litres of M9 solution for collecting and washing </span><span style = "font-style:italic;">C. elegans</span><span style = "font-style:itali... | ["AB11Dissolve ingredients in 8L double distilled H2O22Adjust volume to10L using double distilled H2O33Measure or use pump to dispense aliquots accurately. 44Label, date and autoclave. 55After autoclaving, add 100ul 1M MgSO4 per 100ml. (ie. 100ul to 100ml and 200ul to 200ml bottles).6 7NB1M MgSO4 at 1ul... |
82,649 | Collecting Bark and Ambrosia Beetles | 3 | dx.doi.org/10.17504/protocols.io.5jyl855x7l2w/v3 | https://www.protocols.click/view/collecting-bark-and-ambrosia-beetles-cuxzwxp6 | Jiri Hulcr, Andrew J. Johnson, Demian F Gomez | TITLE: Collecting Bark and Ambrosia Beetles
AUTHORS: Jiri Hulcr, Andrew J. Johnson, Demian F Gomez
[DESCRIPTION]
This protocol describes the different methods to collect and preserve bark ambrosia beetles.
This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordination Network. For more information on... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.t4seqwe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Odontogenic lesions are divided into odontogenic cysts and tumors. They affect the osseous marrow and cortex of the jaw bones, and are uniquely derived from the tissues of developing teeth. Odontogenic cysts are cavities lined by odontogenic derived epithelium. They are the most... | [] |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.