id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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103,199 | Mouse synapse imaging and analysis | 0 | dx.doi.org/10.17504/protocols.io.14egn6mzpl5d/v1 | https://www.protocols.io/view/mouse-synapse-imaging-and-analysis-dgz73x9n | Shiyi Wang | TITLE: Mouse synapse imaging and analysis
AUTHORS: Shiyi Wang
[DESCRIPTION]
Mouse synapse imaging and analysis
[STEPS]
1. **Tissue Sectioning and Preparation**
1.1. - Prepare coronal sections of 30 μm thickness containing the anterior cingulate cortex (ACC) and primary motor cortex (MOp) from WT and LRRK2 G2019Ski/ki... | ["**Tissue Sectioning and Preparation**", "- Prepare coronal sections of 30 μm thickness containing the anterior cingulate cortex (ACC) and primary motor cortex (MOp) from WT and LRRK2 G2019Ski/ki mice.", "**Synaptic Staining**", "- Perform synaptic staining using the following antibody combinations:", "- Excitatory (I... |
95,890 | Rotarod | 0 | dx.doi.org/10.17504/protocols.io.36wgq3xmolk5/v1 | https://www.protocols.io/view/rotarod-c9vsz66e | sdwalto, Bryan_Killinger, Jeffrey Kordower | TITLE: Rotarod
AUTHORS: sdwalto, Bryan_Killinger, Jeffrey Kordower
[DESCRIPTION]
Rotarod protocol optimized for mice. This test is made to assess motor coordination and balance. The results should show lower latency to fall numbers for mice with motor impairments.
[STEPS]
1. Training: Each mouse was given a training... | ["Training: Each mouse was given a training session. Four 5 min trials, 5 mins apart.", "Test Period (1 hour later): Each mouse was place inside the rotarod apparatus with increasing speed (4 rpm to 40 rpm in 300 secs). Latency to fall was recorded. (Digital rotarod recorded it automatically)", "Each mouse received two... |
92,732 | Antibody Purification and Labeling | 1 | dx.doi.org/10.17504/protocols.io.kxygx34rdg8j/v1 | https://www.protocols.io/view/antibody-purification-and-labeling-c6s4zegw | Maya Brewer, Yuantee Zhu, Jeff Spraggins, Mark De Caestecker | TITLE: Antibody Purification and Labeling
AUTHORS: Maya Brewer, Yuantee Zhu, Jeff Spraggins, Mark De Caestecker
[DESCRIPTION]
This protocol describes the process for antibody purification, subsequent labeling for direct immunofluorescence, and validating antibody labeling.
[STEPS]
SECTION: Antibody Purification
2. Pr... | ["[Antibody Purification] Prepare buffers from AB buffer kit:", "[Antibody Purification] This is to remove any BSA, azide, glycine that is often added by the\nmanufacturer for stabilization. If the antibody has nothing added, skip this section.", "[Antibody Purification] 10X binding buffer must be diluted to 1X (9 part... |
null | null | null | dx.doi.org/10.17504/protocols.io.u4eeyte | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol describe step by step how to download and explore the dataset provided by the article Three-dimensional nanostructure of an intact microglia cell of Bolasco et al 2018.
[BEFORE_START]
In this protocol we describe how to navigate the dataset with Blender. Because i... | ["[Do the downloads and installs] {\"blocks\":[{\"key\":\"5bpu3\",\"text\":\"Download the dataset\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"25q3v\",\"text\":\"\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"... |
null | null | null | dx.doi.org/10.17504/protocols.io.hfjb3kn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Sabrina Geraci-Yee modified this protocol from: Pozarowski P., Darzynkiewicz Z. 2004. Analysis of cell cycle by flow cytometry. Methods Mol.<br />Bio. 281:301-311.</p>
[STEPS]
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104,356 | Exploring Uptake and Engagement with Community Food-Related Services in Low Income Areas Using the COM-B Model | 0 | dx.doi.org/10.17504/protocols.io.8epv5rd96g1b/v1 | https://www.protocols.io/view/exploring-uptake-and-engagement-with-community-foo-dh6c39aw | Abigail Stephen, Julia Allan, Oana Petre, Janet Kyle, Frank Thies | TITLE: Exploring Uptake and Engagement with Community Food-Related Services in Low Income Areas Using the COM-B Model
AUTHORS: Abigail Stephen, Julia Allan, Oana Petre, Janet Kyle, Frank Thies
[DESCRIPTION]
Background: Low socioeconomic status (SES) is a significant risk factor for obesity and related non-communicable... | ["[Background and Study Design] Aim:\n\nIn the present study, the aim is to understand and quantify the perspective of users and non-users of community dietary interventions within low-income communities, specifically what barriers and\nfacilitators are associated with their uptake and engagement and consumption of\na ... |
44,138 | selection of Participants | 5 | dx.doi.org/10.17504/protocols.io.bpcimiue | https://www.protocols.io/view/selection-of-participants-bpcimiue | memirabaral | TITLE: selection of Participants
AUTHORS: memirabaral
[STEPS]
?. [S1 Fig]
Selection of participants for the study | ["[S1 Fig]\nSelection of participants for the study"] |
97,922 | tocsy_metab.nan | 5 | dx.doi.org/10.17504/protocols.io.x54v92meml3e/v1 | https://www.protocols.io/view/tocsy-metab-nan-dbva2n2e | NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison | TITLE: tocsy_metab.nan
AUTHORS: NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison
[DESCRIPTION]
This is a protocol for running the Bruker pulse program "dipsi2gpphpr" for metabolomics samples.
[BEFORE_START]
This protocol assumes:
Your sample is l... | ["[Create a new dataset]", "[Create noesypr1d experiment file] When Lock, Tune, Shim are complete proceed to the specific protocol for your desired pulseprogram(s).", "[Acquire a spectrum] Go to the \"USE DEFAULT\" tab below to proceed with the default optimized parameters.", "[Create a new dataset] On the menu bar on ... |
null | null | null | dx.doi.org/10.17504/protocols.io.f5mbq46 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
66,195 | Transform pCas9 | 4 | dx.doi.org/10.17504/protocols.io.eq2lynbopvx9/v1 | https://www.protocols.io/view/transform-pcas9-ccvtsw6n | Qiaowa Gong | TITLE: Transform pCas9
AUTHORS: Qiaowa Gong
[DESCRIPTION]
this protocol is aimed to provide instructions of transforming pCas9, which is a key step of knock-out gene by Crispr/Cas9
[STEPS]
1. Mix 50 µL of competent MG1655 with 10 µL of pCas9
2. Keep the reaction mix on ice for30 min
3. Then, transfer the mix to ... | ["Mix 50 µL of competent MG1655 with 10 µL of pCas9", "Keep the reaction mix on ice for30 min", "Then, transfer the mix to the water bath set at 42 °C for 45 s", "Next, keep the mix on the ice for 2 min", "Add 940 µL LB broth and incubate the cells at 37 °C with vigorous shaking (200 rpm) for 60 min", "Plate the cell... |
null | null | null | dx.doi.org/10.17504/protocols.io.dqp5vm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For use in "Obtaining pure cyanophage stocks (liquid assay)"
[STEPS]
?.
?.
?.
?.
?.
?.
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?. | [] |
100,224 | U2OS Nucleofection & Analysis Protocol for MSPH Validation | 0 | null | https://www.protocols.io/view/u2os-nucleofection-amp-analysis-protocol-for-msph-dd4828zw | Jason Waligorski, Colin Kremitzki, Graham Bachman, Mallory Wright, William J Buchser | TITLE: U2OS Nucleofection & Analysis Protocol for MSPH Validation
AUTHORS: Jason Waligorski, Colin Kremitzki, Graham Bachman, Mallory Wright, William J Buchser
[DESCRIPTION]
Validation steps.
[STEPS]
SECTION: Design
1. Choosing synGRNA sequence
This is the method utilized in 2023 for the MSPH library in U2OS ce... | ["[Design] Choosing synGRNA sequence\n\nThis is the method utilized in 2023 for the MSPH library in U2OS cells for validating Raft-Seq hits. This is NOT the tandem gRNA method.\n\nChoose one gRNA that was a hit from the primary screen, usually from a published library (Brunello)\nDesign a 2nd 'backup' gRNA sequenced wh... |
93,734 | Protocol TE Display sequencing (TED-Seq) | 4 | null | https://www.protocols.io/view/protocol-te-display-sequencing-ted-seq-c7seznbe | Quadrana Leandro, pol.vendrell, basile.leduque | TITLE: Protocol TE Display sequencing (TED-Seq)
AUTHORS: Quadrana Leandro, pol.vendrell, basile.leduque
[DESCRIPTION]
Background: Mobilization of transposable elements (TEs) can generate large effect mutations. However, because transposition is typically rare, the actual rate and landscape of new insertions remains un... | ["[Before Starting] Before you start,be sure to have:\n\nP7_adapter_up\nP7_adapter_bottom\nP7 primer (P7_Primer_index#)\nPrimer for the 1st PCR (outer Primers)\nPrimer for the nested PCR (P7_Primer_index#, TE Specific P5adapter)\n\nNEBNext® UltraTM II DNA Library Prep Kit for Illumina® (E7645, E7103)", "[End repair and... |
51,194 | pA-Hia5 Protein Expression and Purification | 4 | dx.doi.org/10.17504/protocols.io.bv82n9ye | https://www.protocols.io/view/pa-hia5-protein-expression-and-purification-bv82n9ye | Nicolas Altemose, Annie Maslan, Owen Smith, Kousik Sundararajan, Rachel Brown, Aaron Straight, Aaron Streets | TITLE: pA-Hia5 Protein Expression and Purification
AUTHORS: Nicolas Altemose, Annie Maslan, Owen Smith, Kousik Sundararajan, Rachel Brown, Aaron Straight, Aaron Streets
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is a protocol for the purification of protein A-Hia5 (pA-Hia5), protein... | ["[Induction]\nTransform pA-Hia5 into T7 Express lysY competent E. coli cells for recombinant protein expression.", "[Purification]\nOn the day of purification, thaw cells (store on ice) and add 2x EDTA-free protease inhibitor tablet and 10 mM β-mercaptoethanol per 2 L of pelleted cell culture.", "[Induction]\nDilute 2... |
82,125 | L1 stage C. elegans dissociation for FACS isolation and RNA-seq analysis of intestine-specific cells | 4 | dx.doi.org/10.17504/protocols.io.rm7vzy365lx1/v2 | https://www.protocols.io/view/l1-stage-c-elegans-dissociation-for-facs-isolation-cufmwtk6 | Robert TP Williams, Erin Osborne Nishimura | TITLE: L1 stage C. elegans dissociation for FACS isolation and RNA-seq analysis of intestine-specific cells
AUTHORS: Robert TP Williams, Erin Osborne Nishimura
[DESCRIPTION]
This protocol is for generating a single cell suspension suitable for isolation of intestine-specific cells through Fluorescence Activated Cell S... | ["[Before beginning] Prepare reagents in advance\n\n\nL15-10 Buffer: Mix 500 ml Leibovitz's L-15 Medium, 50 ml Fetal Bovine Serum (heat inactivated), 50 ul of 100x Penicillin-Streptomycin solution and 7.7 g sucrose. Filter with 0.2 micron pore filter. Store at 4ºC.\n\nEgg Buffer: Mix 29.5 ml of 2M NaCl, 12 ml of 2M KCl... |
63,389 | Trim Clinical Keto Reviews – An Excellent Fat-Burning Ketosis Dietary Supplement! | 3 | dx.doi.org/10.17504/protocols.io.3byl4bd2jvo5/v1 | https://www.protocols.io/view/trim-clinical-keto-reviews-an-excellent-fat-burnin-b955r886 | Trim Clinical Keto | TITLE: Trim Clinical Keto Reviews – An Excellent Fat-Burning Ketosis Dietary Supplement!
AUTHORS: Trim Clinical Keto
[DESCRIPTION]
Trim Clinical Keto Reviews: Pills can make your weight reduction routine simpler than any time in recent memory! This effective recipe has the ability to drive your body into ketosis. What... | [] |
70,288 | OT-2 Counter-Selection | 5 | dx.doi.org/10.17504/protocols.io.5qpvor5xdv4o/v1 | https://www.protocols.io/view/ot-2-counter-selection-cgvqtw5w | Ana Mariya Anhel, Lorea Alejaldre, Ángel Goñi-Moreno | TITLE: OT-2 Counter-Selection
AUTHORS: Ana Mariya Anhel, Lorea Alejaldre, Ángel Goñi-Moreno
[DESCRIPTION]
This protocol is meant to select the samples from 1 source plate that has been growing in different conditions and has different values for these different conditions, one condition gives higher values and the oth... | ["[Files Preparation] Preparing Customized Template\n\nPreparing the template (a .csv) with the specific variables for each experiment.\n\nHere we attach one excel with several sheets:\nTemplate to use in protocol\nExplanation of each variable\nExample", "[Running Protocol] Setting Labware", "[After-Running] Retrieve l... |
38,565 | SARS-CoV-2 EBI submission protocol: ENA, BioSample, and BioProject | 1 | dx.doi.org/10.17504/protocols.io.bhwdj7a6 | https://www.protocols.io/view/sars-cov-2-ebi-submission-protocol-ena-biosample-a-bhwdj7a6 | Nabil-Fareed Alikhan, Ruth Timme, Emma Griffiths, Duncan MacCannell | TITLE: SARS-CoV-2 EBI submission protocol: ENA, BioSample, and BioProject
AUTHORS: Nabil-Fareed Alikhan, Ruth Timme, Emma Griffiths, Duncan MacCannell
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">This protocol provides the steps needed to establish a new EBI subm... | ["[Preamble and other documentation]\nHow data is structured in ENAData in ENA are structured in a hierachy. Data is grouped under a \"STUDY,\" which contains multiple \"SAMPLES\" , which in turn have multiple \"RUNS/EXPERIMENTS\", and from these are derived \"ANALYSES\". STUDY: records overarching information about th... |
66,497 | High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart | 1 | dx.doi.org/10.17504/protocols.io.cc69szh6 | https://www.protocols.io/view/high-resolution-respirometry-of-isolated-mitochond-cc69szh6 | Ana Karen Meza-Buendía, Omar Emiliano Aparicio-Trejo, Fernando Díaz-Herrera, Claudia Caamal-Monsreal, José Pedraza-Chaverri, Carolina Álvarez-Delgado, Kurt Paschke, Carlos Rosas | TITLE: High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart
AUTHORS: Ana Karen Meza-Buendía, Omar Emiliano Aparicio-Trejo, Fernando Díaz-Herrera, Claudia Caamal-Monsreal, José Pedraza-Chaverri, Carolina Álvarez-Delgado, Kurt Paschke, Carlos Rosas
[DESCRIPTIO... | ["[Isolation of mitochondria from the systemic heart of adult octopus] Starve octopus overnight before the isolation experiment.", "[Isolation of mitochondria from the systemic heart of adult octopus] Sacrifice an adult Octopus maya specimen (about 1 kg) previously anesthetized with 3% alcohol and quickly remove the sy... |
43,656 | Ultra-High Macro Photography of Bark Beetles | 3 | dx.doi.org/10.17504/protocols.io.bnvgme3w | https://www.protocols.io/view/ultra-high-macro-photography-of-bark-beetles-bnvgme3w | Demian F Gomez, Andrew J. Johnson | TITLE: Ultra-High Macro Photography of Bark Beetles
AUTHORS: Demian F Gomez, Andrew J. Johnson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol explains the process of taking high quality photos of bark and ambrosia beetles, creating the image stacks, and processing the images in image ... | [] |
54,265 | Xing et al. 2016 Protocol | 1 | null | https://www.protocols.io/view/xing-et-al-2016-protocol-by8zpzx6 | Xing ey al., Ely Bor, Jade Knight | TITLE: Xing et al. 2016 Protocol
AUTHORS: Xing ey al., Ely Bor, Jade Knight
[DESCRIPTION]
Observation on tomatoes and green beans life spans when dipped in a chitosan spray and a combination of the chitosan spray with cinnoman oil. It is the replication of the "Chitosan-Based Coating with Antimicrobial Agents: Prepara... | ["Prepared the CS solution to dissolving CS in an aqueous acetic acid solution at an ambient temperature overnight.", "Added Tween 80 to the solution and Stirred at 45C for 2 hours", "Dropped CEO in to the solution for 30 mins", "Weight ratios of CS to CEO 1:1", "Added drop-wise of TPP solution into o/w emulsion (for C... |
50,922 | High molecular weight bacterial DNA extraction from field-collected fecal samples preserved in ethanol for long-read sequencing | 4 | dx.doi.org/10.17504/protocols.io.bvyin7ue | https://www.protocols.io/view/high-molecular-weight-bacterial-dna-extraction-fro-bvyin7ue | Amandine Magdeleine, Marie-Ka Tilak, Sophie Teullet, Frédéric Delsuc | TITLE: High molecular weight bacterial DNA extraction from field-collected fecal samples preserved in ethanol for long-read sequencing
AUTHORS: Amandine Magdeleine, Marie-Ka Tilak, Sophie Teullet, Frédéric Delsuc
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-al... | ["[1- DNA extraction]\nDNA extraction is done using the “Genomic DNA from soil” kit (NucleoSpin Soil, Macherey-Nagel) from feces preserved in ethanol (96%) at -20°C. Two successive extractions are done to retrieve high molecular weight DNA. The protocol was optimized by using Buffer SL1 + Enhancer SX. Before the extrac... |
80,906 | River water laboratory processing Protocol | 1 | dx.doi.org/10.17504/protocols.io.261ge3mxol47/v1 | https://www.protocols.click/view/river-water-laboratory-processing-protocol-cs9iwh4e | Sneha Shrestha, Christopher LeBoa | TITLE: River water laboratory processing Protocol
AUTHORS: Sneha Shrestha, Christopher LeBoa
[DESCRIPTION]
These are the qPCR conditions used for the S. Typhi and Paratyphi qPCR used for environmental surveillance by our lab team
[STEPS]
SECTION: Turbidity Testing
1. 2ml of sample (from 5ml separated for Phage Screen... | ["[Turbidity Testing] 2ml of sample (from 5ml separated for Phage Screening) is taken in a sterile glass\r\ntube", "[Turbidity Testing] Turbidity is measured by densitometry.", "[Differential Centrifugation] Spin down 45ml of collected water sample at 2000rpm for 1 min to separate out larger\r\ndebris.", "[Differential... |
null | null | null | dx.doi.org/10.17504/protocols.io.mqnc5ve | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This questionnaire serves to interview subjects undergoing rehabilitation of joint movement by means of exoskeleton robot HAL (hybrind assistive limbs). The goal is to learn about subjective control strategies during voluntary initiation of joint movement. Questions and multi... | [] |
59,811 | Measurement of pancreatic islet beta-cell proliferation by flow cytometry | 4 | dx.doi.org/10.17504/protocols.io.n92ldz8d9v5b/v1 | https://www.protocols.io/view/measurement-of-pancreatic-islet-beta-cell-prolifer-b6nbrdan | Julien Ghislain, Vincent Poitout | TITLE: Measurement of pancreatic islet beta-cell proliferation by flow cytometry
AUTHORS: Julien Ghislain, Vincent Poitout
[DESCRIPTION]
This protocol describes the steps to assess pancreatic beta-cell proliferation by flow cytometry. It is suitable for islets isolated from both rodents and humans. We routinely appl... | ["[Islet dispersion and fixation] Dispersing islets into single cells\nWash approximately 100-200 islets two times with ice cold PBS containing 2 millimolar (mM) EDTA (pH 8) in a 1.5 ml microcentrifuge tube using a microcentrifuge at 339 x g, 3 min, 4 °C. \nKeeping the tubes on ice add 200 µL-300 µL of ice cold Accutas... |
55,832 | Intracardial perfusion of the African turquoise killifish | 4 | dx.doi.org/10.17504/protocols.io.b2ryqd7w | https://www.protocols.io/view/intracardial-perfusion-of-the-african-turquoise-ki-b2ryqd7w | Valerie Mariën, Jolien Van houcke, Lutgarde Arckens | TITLE: Intracardial perfusion of the African turquoise killifish
AUTHORS: Valerie Mariën, Jolien Van houcke, Lutgarde Arckens
[DESCRIPTION]
This perfusion protocol is essential for preserving tissue morphology in order to perform good quality immunohistochemical stainings. Here, we show you how we perform our per... | ["[Preparation] Prepare 0.1% tricaine solution in reverse osmosis water.\nPrepare 4% paraformaldehyde (PFA) solution in PBS.\nDisinfect all dissection material with 70 % EtOH.\nCut the needle off the Terumo Needle Surflo Winged infusion set.", "[Preparation] Fill one of the 10 ml syringes with PBS and the other one wit... |
22,875 | SPARC Long-term exposure to intermittent hypoxia (or normoxia) using a custom in-cage computer controlled system | null | dx.doi.org/10.17504/protocols.io.2j3gcqn | null | Elisa Gonzalez-Rothi, Latoya Allen, Alec Simon, Yasin Seven, Marissa Ciesla, Gordon Mitchell | TITLE: SPARC Long-term exposure to intermittent hypoxia (or normoxia) using a custom in-cage computer controlled system
AUTHORS: Elisa Gonzalez-Rothi, Latoya Allen, Alec Simon, Yasin Seven, Marissa Ciesla, Gordon Mitchell
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the pr... | ["Rats are exposed to either long-term normoxia or IH treatments (various protocols) for up to 28 days", "Rats are housed in custom-designed PlexiGlas gas exposure cages (Volume~16L) with free access to food and water", "Cages are fitted with a custom cage top equipped to deliver protocol-specific gas (O2 balanced with... |
82,530 | Research on ERIH PLUS approved SSH journals present in OpenCitations Meta database | 5 | dx.doi.org/10.17504/protocols.io.5jyl8jo1rg2w/v3 | https://www.protocols.click/view/research-on-erih-plus-approved-ssh-journals-presen-cuuawwse | Ali Ghasempouri, maddalena.ghiotto, sebastiano.giacomini | TITLE: Research on ERIH PLUS approved SSH journals present in OpenCitations Meta database
AUTHORS: Ali Ghasempouri, maddalena.ghiotto, sebastiano.giacomini
[DESCRIPTION]
In this study, we present a comprehensive workflow to assess the coverage of publications in Social Science and Humanities (SSH) journals indexed in... | ["[Retrieve OpenCitation Meta publication and Journals that are registered in ERIH-PLUS index] Starting from the ERIH-PLUS index of Social Science and Humanities approved journals dataset \n (downloaded 27/04/2023) we want to retrieve all the publications belonging to one of those journals, included in OpenCitations M... |
81,132 | F-2 FECES PROCESSING | 4 | dx.doi.org/10.17504/protocols.io.kxygx9ddog8j/v1 | https://www.protocols.io/view/f-2-feces-processing-ctgkwjuw | REDI-NET Consortium | TITLE: F-2 FECES PROCESSING
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol details feces processing.
[BEFORE_START]
BEFORE START
Pre-cool the Bullet Blender by adding dry ice into the cooling compartment and running the cooling program.
Pre-heat the heater mixer at 95 °C.
Clean the work surfaces with RNa... | ["[1. SAMPLE LYSIS] Add 700 µL of ASL buffer to the bead tubes prepared in step 4 from Before Start under the Guidelines & Warning tab.", "[1. SAMPLE LYSIS] Use a clean spatula or forceps to weigh about 100 mg fecal sample (roughly 5 drops of mouse feces) and place it into each prepared bead tube (If fecal sample is fr... |
78,326 | Institutional Linkages Between National Social Health Protection And Occupational Health Services Systems: A Scoping Review | 1 | dx.doi.org/10.17504/protocols.io.rm7vzbqw2vx1/v1 | https://www.protocols.io/view/institutional-linkages-between-national-social-hea-cqqwvvxe | Aurore Iradukunda, Marietou Niang, Lou Tessier, Tatiana Agbadje, Gloria Ayivi-Vinz, Ana Catalina Ramirez, Frédéric Bergeron, Dejan Loncar | TITLE: Institutional Linkages Between National Social Health Protection And Occupational Health Services Systems: A Scoping Review
AUTHORS: Aurore Iradukunda, Marietou Niang, Lou Tessier, Tatiana Agbadje, Gloria Ayivi-Vinz, Ana Catalina Ramirez, Frédéric Bergeron, Dejan Loncar
[DESCRIPTION]
The impact of globalization... | ["[Description of the study] Establishing robust coordination mechanisms between social health protection (SHP) and occupational health services (OHS) embedded in the national institutional architecture can strengthen and scale up both systems. However, there is limited evidence of inter-institutional linkages, even in... |
87,929 | Modified one-step growth (mOSG) assay | 4 | dx.doi.org/10.17504/protocols.io.4r3l2op9pv1y/v3 | https://www.protocols.io/view/modified-one-step-growth-mosg-assay-cz4zx8x6 | Eva JP. Lievens | TITLE: Modified one-step growth (mOSG) assay
AUTHORS: Eva JP. Lievens
[DESCRIPTION]
This protocol describes a modified one-step growth assay, as presented in the manuscript "Efficient assays to quantify the life history traits of algal viruses" (Lievens et al. 2023, Applied & Environmental Microbiology, doi.org/10.112... | ["[Preparation (can be done the day beforehand)] Prepare the 1:1000 dilution plates.", "[Preparation (can be done the day beforehand)] Prepare the 1:10000 dilution plates.", "[Preparation (can be done the day beforehand)] If following the alternative 1:1000 dilution option (see step 9.1):\nPrepare the 1:10 intermediate... |
62,213 | What Are The Ingredients In Superior Nutra Keto And What Do They Do For My Body? | 3 | dx.doi.org/10.17504/protocols.io.dm6gpbxejlzp/v1 | https://www.protocols.io/view/what-are-the-ingredients-in-superior-nutra-keto-an-b8zdrx26 | Superior Nutra Keto | TITLE: What Are The Ingredients In Superior Nutra Keto And What Do They Do For My Body?
AUTHORS: Superior Nutra Keto
[DESCRIPTION]
https://www.mynewsdesk.com/iexponet/pressreleases/superior-nutra-keto-reviews-new-dietary-ingredients-benefits-for-weight-loss-pills-3180600
[STEPS] | [] |
54,519 | Levantamento em bases de dados para Revisões Sistemáticas e Quantitativas da Literatura (RSQL) | 1 | dx.doi.org/10.17504/protocols.io.bzgxp3xn | https://www.protocols.io/view/levantamento-em-bases-de-dados-para-revis-es-siste-bzgxp3xn | Daniel Vartanian | TITLE: Levantamento em bases de dados para Revisões Sistemáticas e Quantitativas da Literatura (RSQL)
AUTHORS: Daniel Vartanian
[DESCRIPTION]
Este protocolo foi criado para o programa de Revisões Sistemáticas e Quantitativas da Literatura (RSQL) científica do Grupo Interdisciplinar de Pesquisa em Sono (GIPSO), mas el... | ["[Preparação] Acesse o VPN da USP", "[Preparação] Abra as planilhas Source e Search", "[Preparação] Preencha os dados iniciais na planilha Search", "[Busca] Acesse o provedor da(s) base(s) de dados", "[Busca] Selecione somente as bases de dados que irá utilizar (se aplicável)", "[Busca] Limpe o histórico de busca", "[... |
82,335 | Mouse brain slice electrophysiology | 3 | dx.doi.org/10.17504/protocols.io.n92ldp44nl5b/v1 | https://www.protocols.io/view/mouse-brain-slice-electrophysiology-cum7wu9n | Shiyi Wang | TITLE: Mouse brain slice electrophysiology
AUTHORS: Shiyi Wang
[DESCRIPTION]
Mouse brain slice electrophysiology
[STEPS] | [] |
64,505 | GRAB sensor imaging in mouse striatal slices | 4 | dx.doi.org/10.17504/protocols.io.8epv59ow5g1b/v1 | https://www.protocols.io/view/grab-sensor-imaging-in-mouse-striatal-slices-ca8zshx6 | Stefania Vietti-Michelina, Ross McLeod, Shinil Raina, Yanfeng Zhang, Stephanie J Cragg | TITLE: GRAB sensor imaging in mouse striatal slices
AUTHORS: Stefania Vietti-Michelina, Ross McLeod, Shinil Raina, Yanfeng Zhang, Stephanie J Cragg
[DESCRIPTION]
This protocol describes the steps to image GRAB sensors in mouse striatal slices.
[BEFORE_START]
Stereotaxic intracranial injections were performed to deliv... | ["[Slice Preparation]", "[Slice Preparation] Sacrifice mice by cervical dislocation and exsanguination, and collect the brain.", "[Slice Preparation] Cut 300 μm thick coronal slices using a vibratome in ice-cold HEPES-based buffer saturated with 95% O2/ 5% CO2, containing (in mM): 120 NaCl, 20 NaHCO3, 6.7 HEPES acid, 5... |
97,844 | Ventral Midbrain Genomic PCR | 1 | dx.doi.org/10.17504/protocols.io.kqdg324zzv25/v1 | https://www.protocols.io/view/ventral-midbrain-genomic-pcr-dbsu2new | madalynn.erb Erb | TITLE: Ventral Midbrain Genomic PCR
AUTHORS: madalynn.erb Erb
[DESCRIPTION]
This protocol details ventral midbrain genomic PCR.
[STEPS]
SECTION: Brain tissue collection
1. Euthanize mouse via cervical dislocation.
SECTION: Brain tissue collection
2. Isolate coronal midbrain section using stainless steel brain matri... | ["[Brain tissue collection] Euthanize mouse via cervical dislocation.", "[Brain tissue collection] Isolate coronal midbrain section using stainless steel brain matrix (Stoelting 51386).", "[Brain tissue collection] Remove cortex and dorsal midbrain tissue.", "[Brain tissue collection] Separate ipsilateral and contral... |
44,539 | Video protocol for sorting Drosophila pupae | 4 | dx.doi.org/10.17504/protocols.io.bpq3mmyn | https://www.protocols.io/view/video-protocol-for-sorting-drosophila-pupae-bpq3mmyn | Carolyn Elya | TITLE: Video protocol for sorting Drosophila pupae
AUTHORS: Carolyn Elya
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is my favorite way to sort pupae.</div><div class = "text-block">Credit to Tom Alisch & Dave Zucker (@FlySorter) for discovering a new use for transparencies!!</div></div>
[... | ["Video summary of the method:", "Cut out a small piece of a projector sheet (e.g. Hygloss Overhead Projector Sheets, https://www.amazon.com/Hygloss-75910-Products-Transparency-Projectors/dp/B077762QK5/ref=sr_1_8?dchild=1&keywords=transparency&qid=1605473049&sr=8-8). You'll want to make sure the piece is long enough to... |
49,555 | Determination of lignin in lignocellulosic biomass | 6 | dx.doi.org/10.17504/protocols.io.bumtnu6n | https://www.protocols.io/view/determination-of-lignin-in-lignocellulosic-biomass-bumtnu6n | Magdiel Lainez | TITLE: Determination of lignin in lignocellulosic biomass
AUTHORS: Magdiel Lainez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Explanation of NREL/TP-510-42618</span><span style = "vertical-align:super;">1</span><span> laboratory analytical procedure.</span></div><div class = "text-block"><... | ["[Preparation of biological material]\nFirst, mill the material until reaching a particle size between and for better results.If previously it's necessary to wash the material use distiller water. Do this with the objective to remove starch, gums, and non-structural carbohydrates. Then dry the biomass.\n0.25\n0.425... |
63,421 | Proto 1 | 1 | dx.doi.org/10.17504/protocols.io.ewov1nx87gr2/v1 | https://www.protocols.io/view/proto-1-b965r9g6 | mark | TITLE: Proto 1
AUTHORS: mark
[DESCRIPTION]
Proto sub
[STEPS]
1.
2. asdadsdas
3. asdasdasdas
4. dasdasdasdasd
5. asdasdasdasd | ["asdadsdas", "asdasdasdas", "dasdasdasdasd", "asdasdasdasd"] |
null | null | null | dx.doi.org/10.17504/protocols.io.cjuunv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is for First Strand Synthesis with Reverse Transcriptase
[STEPS]
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49,677 | Accelerated High-Yield Generation of Limb-Innervating Motor Neurons from Human Stem Cells | 1 | dx.doi.org/10.17504/protocols.io.burmnv46 | https://www.protocols.io/view/accelerated-high-yield-generation-of-limb-innervat-burmnv46 | Mackenzie W. Amoroso, Gist F. Croft, Damian J. Williams, Sean O’Keeffe, Monica A. Carrasco, Anne R. Davis, Laurent Roybon, Derek H. Oakley, Tom Maniatis, Christopher E. Henderson | TITLE: Accelerated High-Yield Generation of Limb-Innervating Motor Neurons from Human Stem Cells
AUTHORS: Mackenzie W. Amoroso, Gist F. Croft, Damian J. Williams, Sean O’Keeffe, Monica A. Carrasco, Anne R. Davis, Laurent Roybon, Derek H. Oakley, Tom Maniatis, Christopher E. Henderson
[DESCRIPTION]
<div class = "text-b... | ["[Cell culture maintenance]\nMaintain all cell cultures at , 5% CO2.\n37 °C", "[Cell culture maintenance]\nMaintain hES and iPS cells [HUES3 (control), male; H9 (control), female; HS001 (ALS-SOD1 N139K), male; LWM002 (ALS-SOD1 A4V), female; MBN007 (ALSSOD1 A4V), female; TM008 (ALS-SOD1 A4V), female; DCM009 (ALSSOD1 V1... |
null | null | null | dx.doi.org/10.17504/protocols.io.ejfbcjn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This section outlines the analyses we used in our replication cycle section of our report. We first predict how many contigs are potentially of the temperate replication cycle and display this information using a Euler diagram. We then use a relative abundance approach by visual... | [] |
41,423 | Home-Brew SPRI Beads | 4 | dx.doi.org/10.17504/protocols.io.bkppkvmn | https://www.protocols.io/view/home-brew-spri-beads-bkppkvmn | Sarah Boswell | TITLE: Home-Brew SPRI Beads
AUTHORS: Sarah Boswell
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The is a protocol to make a substitute for AMPure XP that is of equal effectiveness in comparison to the commercial product but far more cost-effective.</div><div class = "text-block">Credit for this g... | ["[Make TE solution (10mM TrisHCl, 1mM EDTA)]\nMake TE solution (10mM Tris-HCl, 1mM EDTA pH 8) 1M Tris-HCl pH 8 0.5M EDTA Nuclease free water\n500 µl\n100 µl\n49.6 mL", "[Wash SpeedBeads]\nMix SpeedBead bottle thoroughly by shaking and vortexing.", "[Wash SpeedBeads]\nTransfer of SpeedBeads to a 1.5ml microtube.\n1... |
76,150 | Indexing PCR and purification of dsDNA libraries | 1 | dx.doi.org/10.17504/protocols.io.rm7vzboy4vx1/v1 | https://www.protocols.io/view/indexing-pcr-and-purification-of-dsdna-libraries-cnkwvcxe | Marcel Keller, Christiana L Scheib, Biancamaria Bonucci | TITLE: Indexing PCR and purification of dsDNA libraries
AUTHORS: Marcel Keller, Christiana L Scheib, Biancamaria Bonucci
[DESCRIPTION]
Protocol for the indexing PCR and purification of dsDNA libraries, optimized for ultra-short ancient DNA molecules, modified from Meyer & Kircher (2010) Cold Spring Harb. Protoc. (doi:... | ["[PCR] In the modern lab, place the PCR strips in the cycler and run the following program:\n \n StepTime [min:sec]Temperature [°C]CyclesPreincubation5:00941Melting0:309415Annealing0:3060Elongation0:3068Hold7:00721Coolinginfinite41", "[Purification] Turn on the heat block 37 °C for the elution.", "[Purification] ... |
null | null | null | dx.doi.org/10.17504/protocols.io.g2vbye6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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100,145 | 10x v3.1 HT Genomics Sample Processing Protocol | 0 | dx.doi.org/10.17504/protocols.io.ewov19o6klr2/v1 | https://www.protocols.io/view/10x-v3-1-ht-genomics-sample-processing-protocol-dd2r28d6 | Allen Institute | TITLE: 10x v3.1 HT Genomics Sample Processing Protocol
AUTHORS: Allen Institute
[DESCRIPTION]
Protocol is used for rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells. Doubling the capacity of the original 10xv3.1 protocol, allowing for a capture of ~20,0000 cells instead of ~10,... | [] |
42,737 | Isolation and detection of CTCs in HCC pat | 1 | dx.doi.org/10.17504/protocols.io.bmyrk7v6 | https://www.protocols.io/view/isolation-and-detection-of-ctcs-in-hcc-pat-bmyrk7v6 | Linda Riegelbauer | TITLE: Isolation and detection of CTCs in HCC pat
AUTHORS: Linda Riegelbauer
[STEPS]
?. - Blood from Cellsave Preservative Tubes was transferred into 50 ml tubes and made up to the 15 ml mark with PBS (without Ca2+ and Mg2+)
?. - this was transferred to the filter of the OncoQuick tube.
?. - Centrifuge this OncoQuick... | ["- Blood from Cellsave Preservative Tubes was transferred into 50 ml tubes and made up to the 15 ml mark with PBS (without Ca2+ and Mg2+)", "- this was transferred to the filter of the OncoQuick tube.", "- Centrifuge this OncoQuick tube for 20 minutes at 1600g and +4 °C. The acceleration as well as the deceleration o... |
40,412 | Universal sandwich enzyme linked immunosorbent assay for investigating protein-LG (SpLG) interactions with immunoglobulins using a SpA-HRP conjugate. | 6 | dx.doi.org/10.17504/protocols.io.bjp4kmqw | https://www.protocols.io/view/universal-sandwich-enzyme-linked-immunosorbent-as-bjp4kmqw | Angel Justiz-Vaillant, Norma McFarlane-Anderson | TITLE: Universal sandwich enzyme linked immunosorbent assay for investigating protein-LG (SpLG) interactions with immunoglobulins using a SpA-HRP conjugate.
AUTHORS: Angel Justiz-Vaillant, Norma McFarlane-Anderson
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. This ELISA is used to study the interac... | ["This ELISA is used to study the interaction of a protein-LG (SpLG) with different immunoglobulin preparations from mammalian species.", "The 96 well microtitre plate is coated overnight at 4°C with 2 µg/µl per well of a mixture of protein-L and protein-G in carbonate-bicarbonate buffer pH 9.6.", "Then plate is treate... |
null | null | null | dx.doi.org/10.17504/protocols.io.stmeek6 | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
<p>Clean the benches and all the material that will be used with alcohol 70. </p>
<p>Use tips with filter. </p>
<p>Cool the centrifuge to 4°C.</p>
[STEPS]
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31,395 | Algorithm for gestational age assessment at birth | null | dx.doi.org/10.17504/protocols.io.bawbifan | null | Zilma Reis, Juliano de Souza Gaspar, Sara Oliveira Elias, Regina Amelia Lopes Pessoa De Aguiar | TITLE: Algorithm for gestational age assessment at birth
AUTHORS: Zilma Reis, Juliano de Souza Gaspar, Sara Oliveira Elias, Regina Amelia Lopes Pessoa De Aguiar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol presents an algorithm for gestational age assessment when a reliable last men... | ["[Menstrual cycle reference]\nThe last menstrual period reference\n\nThe interview aims to qualify the information about the last menstrual period, in terms of its reliability.", "[Menstrual cycle reference]\nThe last menstrual period assessment to assist gestation age calculation is based on an interview with women a... |
28,398 | The Preemie-Test for the assessment of the newborn skin maturity | null | dx.doi.org/10.17504/protocols.io.7ynhpve | null | Zilma Reis, Gabriela Luiza Nogueira Vitral, Rodney Nascimento Guimarães, Regina Amélia P L de Aguiar, Roberta Maia C Romanelli | TITLE: The Preemie-Test for the assessment of the newborn skin maturity
AUTHORS: Zilma Reis, Gabriela Luiza Nogueira Vitral, Rodney Nascimento Guimarães, Regina Amélia P L de Aguiar, Roberta Maia C Romanelli
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The authors describe the skin reflection mea... | ["The foot of the newborn examination:Expose the newborn's foot gently. Do not raise the lower limb to perform the exam.Do not handle the newborn. Instead, examine the child where he is being cared for: inside the incubator, warm crib or mother's lap.", "Determining the position of the skin assessment:The Preemie-Test ... |
93,195 | NCBI submission protocol for microbial pathogen surveillance | 1 | dx.doi.org/10.17504/protocols.io.4r3l284pql1y/v10 | https://www.protocols.io/view/ncbi-submission-protocol-for-microbial-pathogen-su-c69jzh4n | Candace Hope Bias, Julie Haendiges, Tina Lusk Pfefer, Errol Strain, Maria Balkey, Ruth Timme | TITLE: NCBI submission protocol for microbial pathogen surveillance
AUTHORS: Candace Hope Bias, Julie Haendiges, Tina Lusk Pfefer, Errol Strain, Maria Balkey, Ruth Timme
[DESCRIPTION]
PURPOSE: This document provides detailed instructions on how to submit whole genome sequence (WGS) data and associated contextual data... | ["[Establish submission environmnet at NCBI] Set up a new NCBI submission environment for your lab:\n\n1.1: Create an NCBI user account\n1.2: Set up an NCBI submission user group for your lab\n1.4: Bookmark the link to your submission portal\n1.5. Identify or establish new BioProjects (detailed in Step 3)\n\n\nReady fo... |
88,417 | Rotarod test for assessing motor coordination and balance (Rat) | 1 | null | https://www.protocols.io/view/rotarod-test-for-assessing-motor-coordination-and-c2j9ycr6 | devilbiss | TITLE: Rotarod test for assessing motor coordination and balance (Rat)
AUTHORS: devilbiss
[DESCRIPTION]
This is a variant of the published Med-Associates protocol used to study the effects of mild TBI on motor performance and balance. The original manual/protocol can be found here: https://med-associates.com/wp-conten... | ["[Daily testing on Rotarod] Setup Rotarod for daily use", "[Daily testing on Rotarod] Turn on Rotarod and sanitize with an IACUC-approved method (e.g. https://peroxigard.com/product-information/wipes/)", "[Daily testing on Rotarod] Verify Rotarod is set to accelerate from 4-40 RPM over 5 Min\n(Setting #9 on ENV-577; M... |
93,651 | Effect of Optimal Movement, Stretching and Strengthening Exercise to Prevent Shoulder Pain of Paraplegic Spinal Cord Injury Patient.d protocol | 1 | dx.doi.org/10.17504/protocols.io.14egn3ojzl5d/v1 | https://www.protocols.io/view/effect-of-optimal-movement-stretching-and-strength-c7ptzmnn | Nadia Afrin Urme | TITLE: Effect of Optimal Movement, Stretching and Strengthening Exercise to Prevent Shoulder Pain of Paraplegic Spinal Cord Injury Patient.d protocol
AUTHORS: Nadia Afrin Urme
[DESCRIPTION]
This study was conducted to determine and compare the effect of optimal movement, stretching, and strengthening exercises with us... | [] |
87,118 | siRNA Transfection of Dispersed Islet Cells | 4 | null | https://www.protocols.io/view/sirna-transfection-of-dispersed-islet-cells-czbnx2me | Amanda Gomes, Xiong Liu, Xiaoqing Dai | TITLE: siRNA Transfection of Dispersed Islet Cells
AUTHORS: Amanda Gomes, Xiong Liu, Xiaoqing Dai
[DESCRIPTION]
siRNA Transfection of Dispersed Islet Cells
[BEFORE_START]
Reconstitute your siRNA to a 20 μM working solution with nuclease-free water.
Work in a cell culture hood, in an RNAse-free environment, and keep... | ["After 2-3 days, perform desired experiment and add 0.5 -1 mL of Trizol to the extra dishes. Freeze cells with Trizol in the -80°C freezer until RNA extraction.", "Wait 4-6h, and add 2 ml of human media (DMEM) to the dishes.", "Add 103 μL of transfection reagents to each dish with 100 μL of media with dispersed cells.... |
88,487 | An end-to-end workflow to study newly synthesized mRNA following rapid protein depletion in Saccharomyces cerevisiae | 4 | dx.doi.org/10.17504/protocols.io.n2bvj3dj5lk5/v1 | https://www.protocols.io/view/an-end-to-end-workflow-to-study-newly-synthesized-c2nfydbn | John B. Ridenour, Rafal Donczew | TITLE: An end-to-end workflow to study newly synthesized mRNA following rapid protein depletion in Saccharomyces cerevisiae
AUTHORS: John B. Ridenour, Rafal Donczew
[DESCRIPTION]
In this protocol, we describe an end-to-end workflow for rapidly degrading a target protein using the AID system and quantifying newly synth... | ["[Yeast growth] Streak appropriate S. cerevisiae strain on YPD agar. Incubate culture at 30°C for 2-3 days.", "[Yeast growth] Transfer an individual colony to 5 ml of YPD. Incubate culture at 30°C with shaking at 220 rpm overnight.", "[Yeast growth] Dilute overnight culture to an OD600 of 0.2 in 40 ml of YPD. Incubate... |
78,163 | Immunolabelling and clearing of intact, fixed rat spinal cord for visualization of neurons projecting to major pelvic ganglion | 1 | dx.doi.org/10.17504/protocols.io.n2bvj8bewgk5/v1 | https://www.protocols.io/view/immunolabelling-and-clearing-of-intact-fixed-rat-s-cqjtvunn | John-Paul Fuller-Jackson, Peregrine B Osborne, Janet R Keast | TITLE: Immunolabelling and clearing of intact, fixed rat spinal cord for visualization of neurons projecting to major pelvic ganglion
AUTHORS: John-Paul Fuller-Jackson, Peregrine B Osborne, Janet R Keast
[DESCRIPTION]
The whole-mount immunolabeling and clearing method (iDISCO) was used to visualize cholera toxin subun... | ["[Spinal cord preparation] While immersed in phosphate buffered-saline (PBS), pH 7.2, trim nerve roots of paraformaldehyde-fixed spinal cord to within approximately 2 mm of the spinal cord surface to facilitate the identification of segments later, following imaging. Segments of particular interest are T13-L2 for the ... |
72,221 | Germination and seedling establishment for deep-flow hydroponics: The benefit of slant boards | 4 | dx.doi.org/10.17504/protocols.io.5jyl89b86v2w/v3 | https://www.protocols.io/view/germination-and-seedling-establishment-for-deep-fl-cir5ud86 | noah.langenfeld, Bruce Bugbee | TITLE: Germination and seedling establishment for deep-flow hydroponics: The benefit of slant boards
AUTHORS: noah.langenfeld, Bruce Bugbee
[DESCRIPTION]
Germination and seedling establishment for transplanting into hydroponics often uses porous substrates, but fine roots grow into these substrates, and they cannot be... | ["[Seeding] Clean a rigid plastic slant board and lay flat on a clean surface.", "[Seeding] Cut a piece of germination paper 10 cm in height and the same length as the slant board.", "[Seeding] Place germination paper on top of slant board.", "[Seeding] Place seeds on top of germination paper (about 9 cm from the botto... |
null | null | null | dx.doi.org/10.17504/protocols.io.evxbe7n | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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null | null | null | dx.doi.org/10.17504/protocols.io.sh4eb8w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong><em>Bodo saltans</em></strong><strong> kill curve protocol using G418 (Gentamicin)</strong></p>
<p><strong> </strong></p>
<p> </p>
<ul>
<li>Gentamycin exhibits toxicity toward both eukaryotic and prokaryotic cells by disrupting ribosome function, thereby blocking the ... | [] |
44,934 | Protocol for scoring animal calls on structural acoustic features prevalent in human music | 1 | dx.doi.org/10.17504/protocols.io.bp5emq3e | https://www.protocols.io/view/protocol-for-scoring-animal-calls-on-structural-ac-bp5emq3e | David Schruth | TITLE: Protocol for scoring animal calls on structural acoustic features prevalent in human music
AUTHORS: David Schruth
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The following protocol was developed using ethnomusicological universals rhythm, tone, interval, repetition, transposition, and sy... | ["[For trainer]\nSample spectrographic repertoires from the literature", "[For trainer]\nScreen capture or scan each figure and name it systematically e.g. genus-species-voc#-name-author-year-figure.png", "[For trainer]\nGenerate a list of vocalizations (in rows) from literature for recording info above (in columns)", ... |
null | null | null | dx.doi.org/10.17504/protocols.io.ekybcxw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p align="LEFT">Stellaris RNA FISH protocol for formalin-fixed, paraffin-embedded (FFPE) tissue.</p>
[BEFORE_START]
<p align="LEFT">Reagents and Equipment</p>
<p align="LEFT">Reagents and Consumables:</p>
<p align="LEFT">a) TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)</p>
<p a... | [] |
33,922 | Immunoprecipitation using Protein A/G Magnetic Beads | 1 | dx.doi.org/10.17504/protocols.io.bddai22e | https://www.protocols.io/view/immunoprecipitation-using-protein-a-g-magnetic-bea-bddai22e | New England Biolabs | TITLE: Immunoprecipitation using Protein A/G Magnetic Beads
AUTHORS: New England Biolabs
[DESCRIPTION]
This protocol explains immunoprecipitation using Protein A/G Magnetic Beads.
[BEFORE_START]
Use 25 µl of Protein A/G Magnetic Beads per 200 µl of crude cell lysate containing 200-500 µg of total protein in a stan... | ["[Cell Lysis] Rinse a 60 mm culture dish of confluent cells with PBS.", "[Cell Lysis] Lyse the cells with 0.5 mL.", "[Cell Lysis] Maintain constant agitation for 30 min at 4 °C.", "[Cell Lysis] Scrape the cells from the dish.", "[Cell Lysis] Sonicate on ice for 5 s; repeat 4 more times:", "[Cell Lysis] Centrifuge for ... |
45,374 | Donor Acceptance Criteria for GE/UPitt HuBMAP Inclusion | 1 | dx.doi.org/10.17504/protocols.io.bqi6muhe | https://www.protocols.io/view/donor-acceptance-criteria-for-ge-upitt-hubmap-incl-bqi6muhe | Ho, Jonhan | TITLE: Donor Acceptance Criteria for GE/UPitt HuBMAP Inclusion
AUTHORS: Ho, Jonhan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This document outlines the required criteria for donor inclusion of skin specimens in the Human BioMolecular Atlas Program (HuBMAP). The study intends to be as inclusive... | ["[DONOR SELECTION]\nSpecimens used will be residual skin from clinical excision procedures. A search in the UPMC laboratory information system for excision specimens from adults in sun-exposed sites and non- sun exposed sites will be performed. Blocks and unstained slides will be created from archived formalin fixed p... |
null | null | null | dx.doi.org/10.17504/protocols.io.msec6be | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span style="font-weight: 400;">We developed a bioinformatic two-step pipeline, so called "MetaMap" pipeline, to screen human RNA-seq datasets for the presence of microbial and viral reads by re-inspecting the non-human-mapping read fraction.</span></p>
<p><span style="font-... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.fs8bnhw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is a modified version of the MoBio PowerPlant Pro DNA extraction kit for plant and lichen tissues that have been stored in CTAB buffer. Prior to being placed in CTAB, photosynthetic tissues from plants and lichens were surface-sterilized with sequential washes ... | [] |
58,594 | MAD4HatTeR | 4 | dx.doi.org/10.17504/protocols.io.14egn779mv5d/v2 | https://www.protocols.io/view/mad4hatter-b5gaq3se | Andres Aranda-Diaz, eric.neubauervickers | TITLE: MAD4HatTeR
AUTHORS: Andres Aranda-Diaz, eric.neubauervickers
[DESCRIPTION]
This protocol has been adapted from Paragon Genomics CleanPlex® NGS Panel
[STEPS]
SECTION: mPCR
1. In a PCR Workstation, prepare mPCR reaction mix
Keep Master Mix and thawed primers on ice
on ice Prepare a mPCR mix for your samples us... | ["[mPCR] In a PCR Workstation, prepare mPCR reaction mix\n\nKeep Master Mix and thawed primers on ice\n\non ice Prepare a mPCR mix for your samples using the instructions below\nMake fresh dilution of primers each time\n\nVortex reagents before using and spin down to collect liquids\n\nAliquot the mix into PCR tubes in... |
33,924 | First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368) | 1 | dx.doi.org/10.17504/protocols.io.x14egnmmv5dy/v2 | https://www.protocols.io/view/first-strand-cdna-synthesis-kit-using-protoscript-bddci22w | New England Biolabs | TITLE: First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368)
AUTHORS: New England Biolabs
[DESCRIPTION]
This protocol explains methods for the "First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368)".
[STEPS]
1. In a sterile microfuge tube add:
3. Add the... | ["In a sterile microfuge tube add:", "Add the following (bringing to 20 µl final volume):", "Incubate at 42 °C for 30 minutes-1 hour.", "Inactivate enzyme at 80 °C for 5 min.", "Store products at -20 °C or proceed to next step(s).", "Optional: Heat for 3-5 minutes at 65 °C-70 °C. Spin briefly and place promptly on ice.... |
67,415 | NTX Nutrition Keto Gummies :- Best Price Are Available! | 4 | dx.doi.org/10.17504/protocols.io.n2bvj67o5lk5/v1 | https://www.protocols.io/view/ntx-nutrition-keto-gummies-best-price-are-availabl-cd3xs8pn | Acv keto Gummies | TITLE: NTX Nutrition Keto Gummies :- Best Price Are Available!
AUTHORS: Acv keto Gummies
[STEPS]
1. Ntx Nutrition Keto Gummies
Product Review: —Ntx Nutrition Keto Gummies Reviews
Used For: — Weight Loss
Health Benefits
Burn extra fat
Better gu... | ["Ntx Nutrition Keto Gummies\nProduct Review: —Ntx Nutrition Keto Gummies Reviews\nUsed For: — Weight Loss\n Health Benefits\n Burn extra fat\n Better gut fitness & promote digestion\n Improves heart fitness\n ... |
40,867 | Schedule of Study Visits and Evaluations (Appendix A of Phase 3 study of Vaccine Candidate for COVID-19) | 1 | dx.doi.org/10.17504/protocols.io.bj6bkran | https://www.protocols.io/view/schedule-of-study-visits-and-evaluations-appendix-bj6bkran | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | TITLE: Schedule of Study Visits and Evaluations (Appendix A of Phase 3 study of Vaccine Candidate for COVID-19)
AUTHORS: Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is Appendix A of "Phase 3 randomized, double-blinded, placebo-con... | [] |
70,660 | Semi-automated tracing of hamstring muscle architecture for B-mode ultrasound images. | 1 | dx.doi.org/10.17504/protocols.io.yxmvm2xbng3p/v1 | https://www.protocols.io/view/semi-automated-tracing-of-hamstring-muscle-archite-cg9ctz2w | Kevin Cronin, Eamonn Delahunt, Shane Foley, Sean Cournane | TITLE: Semi-automated tracing of hamstring muscle architecture for B-mode ultrasound images.
AUTHORS: Kevin Cronin, Eamonn Delahunt, Shane Foley, Sean Cournane
[DESCRIPTION]
Hamstring strains are the most prevalent injury sustained by field-sport athletes. Insufficiencies in the architectural characteristics of the ha... | [] |
96,053 | _ReadMe | 0 | null | https://www.protocols.io/view/readme-c92vz8e6 | Andrew W. Wilson | TITLE: _ReadMe
AUTHORS: Andrew W. Wilson
[DESCRIPTION]
simple ReadMe draft for the workspace.
[STEPS] | [] |
36,864 | Find proteins of unknown function (PUF) using Plantannot - Protocol C | null | dx.doi.org/10.17504/protocols.io.bf88jrzw | https://www.protocols.io/view/find-proteins-of-unknown-function-puf-using-planta-bf88jrzw | Marcos Viana, Adhemar Zerlotini, Mauricio Mudadu | TITLE: Find proteins of unknown function (PUF) using Plantannot - Protocol C
AUTHORS: Marcos Viana, Adhemar Zerlotini, Mauricio Mudadu
[STEPS]
?. [Entering application]
Enter the Plantannot Result's page, with empty filters and text box search: https://www.machado.cnptia.embrapa.br/plantannot/find/?q=Or you can ente... | ["[Entering application]\nEnter the Plantannot Result's page, with empty filters and text box search: https://www.machado.cnptia.embrapa.br/plantannot/find/?q=Or you can enter the https://www.machado.cnptia.embrapa.br/plantannot initial page and click on the magnifying glass with the text box empty as well.", "[Filte... |
null | null | null | dx.doi.org/10.17504/protocols.io.pzqdp5w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>DNA extraction of −80◦C stored leaves by CTAB.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
49,759 | SARS-CoV-2 NCBI submission protocol: SRA, BioSample, and BioProject | 1 | dx.doi.org/10.17504/protocols.io.but7nwrn | https://www.protocols.io/view/sars-cov-2-ncbi-submission-protocol-sra-biosample-but7nwrn | Ruth Timme, Emma Griffiths, Duncan MacCannell, Lee Katz, Michael Weigand | TITLE: SARS-CoV-2 NCBI submission protocol: SRA, BioSample, and BioProject
AUTHORS: Ruth Timme, Emma Griffiths, Duncan MacCannell, Lee Katz, Michael Weigand
[DESCRIPTION]
PURPOSE:
This is a SARS-CoV-2 specific protocol that covers the steps needed to establish a new NCBI submission environment for your laborator... | ["["Ingredients" to have in place before starting your submissions] Set up a new NCBI submission environment for your lab\n1.1: Create an NCBI user account\n1.2: Set up an NCBI submission user group for your lab\n1.4: Bookmark the link to your Submission Portal\n1.5. Identify or establish new BioProjects (det... |
23,014 | Mouse - Stellate Isolation Protocol | null | dx.doi.org/10.17504/protocols.io.2qegdte | null | John Tompkins, Jeffrey Ardell, Kalyanam Shivkumar | TITLE: Mouse - Stellate Isolation Protocol
AUTHORS: John Tompkins, Jeffrey Ardell, Kalyanam Shivkumar
[STEPS]
?. Mouse: C57BL/6JSex: M/FAge: 12 ± 2wks
?. Euthanesia:Overdose isoflurane (5%), decapitation.
?. Materials:4x insect pins1x minutien pins1x surgical tools (scissors, toothed forceps, straight forceps, iridect... | ["Mouse: C57BL/6JSex: M/FAge: 12 ± 2wks", "Euthanesia:Overdose isoflurane (5%), decapitation.", "Materials:4x insect pins1x minutien pins1x surgical tools (scissors, toothed forceps, straight forceps, iridectomy scissors)1x foam dissection board (8 x 10 x 1 inch)1x absorbent pad2x petri dish with Sylgard 500 mls ice co... |
47,796 | ECAM: A low-cost vaping device for collection of electronic cigarette condensate and in vitro studies | 1 | dx.doi.org/10.17504/protocols.io.bswunfew | https://www.protocols.io/view/ecam-a-low-cost-vaping-device-for-collection-of-el-bswunfew | Rosa Campbell, Kelly Burrowes, Vinod Suresh | TITLE: ECAM: A low-cost vaping device for collection of electronic cigarette condensate and in vitro studies
AUTHORS: Rosa Campbell, Kelly Burrowes, Vinod Suresh
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The use of electronic cigarettes (ECs) has become widespread despite many unknowns... | ["[Case Construction]\nThe Structure holds all electrical, mechanical and pneumatic components of ECAM. The parts required are:A1 Acrylic SheetsA2 M6 Threaded RodA3 4xM6 Hex NutsA4 4xM6 WashersA12 M8 x100 mm BoltsConsumablesX5 Electronic CigaretteRecommended ToolsZ1 M3 TapZ2 M3 Tap WrenchZ3 M8 TapZ4 M8 Tap WrenchZ5 Hac... |
null | null | null | dx.doi.org/10.17504/protocols.io.ps8dnhw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
86,278 | T-1 TICK FIELD SAMPLING | 4 | dx.doi.org/10.17504/protocols.io.36wgq3435lk5/v1 | https://www.protocols.io/view/t-1-tick-field-sampling-cyhext3e | REDI-NET Consortium | TITLE: T-1 TICK FIELD SAMPLING
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol describes tick field sampling.
[GUIDELINES]
OBJECTIVE
To clearly document the correct process for effective standardized field collections of ticks, and recommended personal protection measures.
SUMMARY/SCOPE
The overarching ai... | ["[SAMPLING TEAMS] Field sampling of iDNA (tick) samples involves two people. One person serves as the ‘sampler’ and the other person serves as a ‘helper’. The helper can look up details in these instructions when needed, keep track of samples, handle objects that are contamination risks, serve as a second set of eyes ... |
48,493 | Coupling an amino-functionalized oligo to Sepharose beads for tRNA purification | 1 | null | https://www.protocols.io/view/coupling-an-amino-functionalized-oligo-to-sepharos-btkmnku6 | Clark Fritsch | TITLE: Coupling an amino-functionalized oligo to Sepharose beads for tRNA purification
AUTHORS: Clark Fritsch
[DESCRIPTION]
For this protocol we will be coupling a DNA oligo with a 3' amino-modification to N-Hydroxysuccinimidyl-Sepharose® 4 Fast Flow beads (H8280, SIGMA) for use in the purification of specific tRNA is... | ["[Basic Information Regarding tRNA and DNA oligo of Interest] Depending on your tRNA of interest, you will need to order one of the following DNA oligos from IDT or a similar company. When you order the oligos from IDT or a similar company, you should order each oligo at an amount of 5 μmoles and have it purified usin... |
null | null | null | dx.doi.org/10.17504/protocols.io.qs6dwhe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The scope of the protocol is the quantification of pigments in pasture samples in the context of proximal and remote sensing-based studies. The protocol describes a standard procedure for the destructive sampling of mixed pasture covers for the estimation of biophysical varia... | [] |
19,676 | Kings B Media (1L) | null | dx.doi.org/10.17504/protocols.io.xf4fjqw | null | Brian Smith, baltrus@email.arizona.edu micro.tucson | TITLE: Kings B Media (1L)
AUTHORS: Brian Smith, baltrus@email.arizona.edu micro.tucson
[STEPS]
?. [Fill container aproximately 750mL of H2O]
Add stir bar and set to mix
?. [Add Bacto Peptone]
[Bacto Peptone]
[Bacto Tryptone]
[Glycerol]
?. [Add K2HPO4]
or of 1M Solution
1 g
10 ml
?. [Add MGSO4•H2O]
or of 1M Solution
1 ... | ["[Fill container aproximately 750mL of H2O]\nAdd stir bar and set to mix", "[Add Bacto Peptone]\n[Bacto Peptone]\n[Bacto Tryptone]\n[Glycerol]", "[Add K2HPO4]\nor of 1M Solution\n1 g\n10 ml", "[Add MGSO4•H2O]\nor of 1M Solution\n1 g\n10 ml", "[Bring volume to 1L by adding H2O]\nAllow to mix again with stir bar.", "[Ad... |
34,968 | Standard Volvox Medium | null | null | https://www.protocols.io/view/standard-volvox-medium-bedyja7w | Raymond Goldstein, Kyriacos Leptos | TITLE: Standard Volvox Medium
AUTHORS: Raymond Goldstein, Kyriacos Leptos
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol for the execution of a Standard Volvox Medium recipe. Starr's (1969) modification of Provasoli & Pintner's Medium (1959), as further modified by Starr, Kochert... | ["[Autoclaving]\nDispense the mixture as 500 ml batches into desired 1L bottles.", "[Adding Vitamins (Just before use)]\nUsing pre-plugged sterile tips mix the following as a cocktail in a 1.5 ml sterile microcentrifuge:\nAlways add vitamins fresh and keep the medium at .\nBe sure to work under a laminar flow hood of ... |
73,517 | 624.1_URMC_HTC_Cryosectioning Non-Inflated Fresh-Frozen Embedded Lung for Nuclear Isolation or Multi-plexed In Situ Hybridization | 1 | dx.doi.org/10.17504/protocols.io.3byl4j91jlo5/v1 | https://www.protocols.io/view/624-1-urmc-htc-cryosectioning-non-inflated-fresh-f-cj2muqc6 | Gloria S Pryhuber, Cory Poole | TITLE: 624.1_URMC_HTC_Cryosectioning Non-Inflated Fresh-Frozen Embedded Lung for Nuclear Isolation or Multi-plexed In Situ Hybridization
AUTHORS: Gloria S Pryhuber, Cory Poole
[DESCRIPTION]
This protocol describes steps to prepare non-fixed, fresh frozen tissue that has been embedded in OCT, CMC or other embedding ma... | ["Slides may be stored (briefly) at -80 but it is thought to be better to use or, if needed, ship on dry ice, within 24-48 hours of sectioning", "[Tissue Processing – Cutting Fresh Frozen Lung Sections for FISH] Prepare the SuperFrost Plus slides by placing them into a staining rack.", "[Tissue Processing – Cutting Fr... |
99,815 | CODEX FFPE Microtome Sectioning | 0 | dx.doi.org/10.17504/protocols.io.n2bvjn835gk5/v1 | https://www.protocols.io/view/codex-ffpe-microtome-sectioning-ddqf25tn | Joanna Bi | TITLE: CODEX FFPE Microtome Sectioning
AUTHORS: Joanna Bi
[DESCRIPTION]
Protocol for sectioning FFPE blocks for use with CODEX
[STEPS]
1. Place the paraffin blocks on wet ice for 5-10 minutes to rehydrate
2. Heat up a water bath to 40º C
3. Insert a new blade into the microtome
4. Prepare Superfrost Plus Microsocope ... | ["Place the paraffin blocks on wet ice for 5-10 minutes to rehydrate", "Heat up a water bath to 40º C", "Insert a new blade into the microtome", "Prepare Superfrost Plus Microsocope slides (Cat. No: 1255015) next to the microtome", "Place a paraffin block on to the microtome and cut into it until the desired part of th... |
98,707 | cDNA synthesis using the QuantiTect Reverse transcription kit | 0 | dx.doi.org/10.17504/protocols.io.q26g718mkgwz/v1 | https://www.protocols.io/view/cdna-synthesis-using-the-quantitect-reverse-transc-dcmt2u6n | Enrico Bagnoli, Miratul Muqit | TITLE: cDNA synthesis using the QuantiTect Reverse transcription kit
AUTHORS: Enrico Bagnoli, Miratul Muqit
[DESCRIPTION]
This protocol details the synthesis of cDNA.
[STEPS]
SECTION: cDNA synthesis
1. Thaw template RNA on ice. Thaw gDNA wipeout buffer, Quantiscript® Reverse Transcriptase, Quantiscript RT buffer, RT ... | ["[cDNA synthesis] Thaw template RNA on ice. Thaw gDNA wipeout buffer, Quantiscript® Reverse Transcriptase, Quantiscript RT buffer, RT Primer Mix and RNase-free water at room temperature (15 °C25 °C).\n \nMix each solution by flicking the tubes. Centrifuge briefly to collect residual liquid from the sides of the tubes,... |
71,542 | PCR normalization and size selection with magnetic beads | 4 | dx.doi.org/10.17504/protocols.io.q26g7y859gwz/v3 | https://www.protocols.io/view/pcr-normalization-and-size-selection-with-magnetic-ch4wt8xe | Dominik Buchner | TITLE: PCR normalization and size selection with magnetic beads
AUTHORS: Dominik Buchner
[DESCRIPTION]
This protocol describes how to clean up and normalize PCR products or DNA extracts and perform a size selection with carboxylated-magnetic beads and a PEG-NaCl buffer. It works by diluting the beads so that the bindi... | ["Shake the normalization solution until the beads are homogeneously resuspended", "Add 31 µL and 28 µL to a 250 µL U-bottom assay plate", "Add 9 µL", "To bind the DNA to the beads shake at", "Place the plate on a magnet to pellet the beads for 2 min", "Discard the supernatant by pipetting", "With the plate still on ... |
35,308 | Amplification and Pooling | 1 | dx.doi.org/10.17504/protocols.io.beqkjduw | https://www.protocols.io/view/amplification-and-pooling-beqkjduw | Franziska Aron, Guido Brandt | TITLE: Amplification and Pooling
AUTHORS: Franziska Aron, Guido Brandt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the amplification procedure of dual-indexed double-stranded DNA libraries, for shotgun Illumina sequencing. It is typically used for libraries... | ["[Calculations]\nPrepare amplification assay [ ]Based on the quantification results of the indexed libaries (https://dx.doi.org/10.17504/protocols.io.bakticwn) calculate the number of PCR cycles (amplification factor) needed to reach 10^13 copies of DNA per indexed Library.Example: The following calculation is for pe... |
57,820 | Reverse Transcription of Phage RNA | 4 | null | https://www.protocols.io/view/reverse-transcription-of-phage-rna-b4p4qvqw | Frej Larsen | TITLE: Reverse Transcription of Phage RNA
AUTHORS: Frej Larsen
[DESCRIPTION]
Reverse transcription (RT) is the process of turning ssDNA into cDNA using the reverse transcriptase enzyme. This protocol uses the RT reaction to turn ssRNA and dsRNA into ssDNA and is specifically used for virome analysis.
[STEPS]
1. Add... | ["Add 16 µL sample to a PCR tube and denature RNA by heating to 95 °C for 5 min on a ThermoCycler", "Centrifuge for 10 s and put on ice", "Add 4 µL of the 5X master mix to the PCR tube, then run the following program on a ThermoCycler:\n\n25 °C for 10 min (primer annealing)\n50 °C for 10 min (reverse transcriptio... |
null | null | null | dx.doi.org/10.17504/protocols.io.n7ndhme | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<div>
<div>
<p>This protocol was designed and developed at this laboratory.</p>
<p>The assay specifically targets the 3' UTR region of DENV-3 strains and is designed as a qualitative screening test for human cases of DENV-3 infection, but not for infection due to other kno... | [] |
80,498 | NK-92 Cell Culture Protocol | 4 | dx.doi.org/10.17504/protocols.io.rm7vzbdr8vx1/v1 | https://www.protocols.io/view/nk-92-cell-culture-protocol-csuswewe | Creative Bioarray | TITLE: NK-92 Cell Culture Protocol
AUTHORS: Creative Bioarray
[DESCRIPTION]
NK-92 cells are an interleukin-2 (IL-2)-dependent natural killer (NK) cell line derived from peripheral blood mononuclear cells of a 50-year-old white male with acute non-Hodgkin lymphoma.
[GUIDELINES]
NK-92 cells are an interleukin-2 (IL-2)-... | ["[METHODS] Mima basal medium370 mLFetal bovine serum62.5 mLHS (Horse Serum)62.5 mLP/S penicillin-streptomycin5 mLInositol0.2 mM (500 μL)β-mercaptoethanol0.1 Mm (1 mL)Folic acid (folic acid)0.02 mM (180 μL)Recombinant IL-2 interleukin100-200 U/mL (100 μL)", "[METHODS] An incomplete medium is prepared in advance. αMEM+0... |
50,627 | Evaluating intrinsic cardiac neural control of cardiac function using sequential ganglionated plexus ablations | 4 | dx.doi.org/10.17504/protocols.io.bvpbn5in | https://www.protocols.io/view/evaluating-intrinsic-cardiac-neural-control-of-car-bvpbn5in | Peter Hanna, Jeffrey Ardell, Kalyanam Shivkumar | TITLE: Evaluating intrinsic cardiac neural control of cardiac function using sequential ganglionated plexus ablations
AUTHORS: Peter Hanna, Jeffrey Ardell, Kalyanam Shivkumar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The purpose of this protocol is to evaluate the control of the intrinsic ca... | ["[Experimental Set-up]\nPre-sedate a Yucatan minipig via tiletamine-zolazepam () intramuscular injection.\n6", "[Experimental Set-up]\nSedate pig using isoflurane (5% inhalation, initiation) delivered via nosecone mask. Once loss of blink reflex is confirmed, perform endotracheal intubation using a 7.0mm endotracheal ... |
29,267 | Protocol for dissection and tissue preparation of the spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae) for morphometric analysis and morphological analysis using scanning electron microscopy | null | dx.doi.org/10.17504/protocols.io.8tthwnn | null | Alina Avanesyan, Timothy K. Maugel [Laboratory for Biological Ultrastructure, William O. Lamp [Department of Entomology | TITLE: Protocol for dissection and tissue preparation of the spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae) for morphometric analysis and morphological analysis using scanning electron microscopy
AUTHORS: Alina Avanesyan, Timothy K. Maugel [Laboratory for Biological Ultrastructure, William O. Lamp [Depa... | [] |
74,082 | Screening procedure to identify triazole-resistant Aspergillus spp. using agar plates | 1 | null | https://www.protocols.io/view/screening-procedure-to-identify-triazole-resistant-ckkauuse | Juliê JA Alves | TITLE: Screening procedure to identify triazole-resistant Aspergillus spp. using agar plates
AUTHORS: Juliê JA Alves
[DESCRIPTION]
The methodology of the screening procedure for detecting resistance to azoles in the genus Aspergillus spp. consists of an in house technique, with visual analysis of fungal growth on aga... | ["[Preparation of antifungal solutions:] From the antifungal powder prepare stock solutions in DMSO, at concentrations at least 200 times higher than those tested on the agar plate", "[Preparation of antifungal solutions:] From the stock solution, prepare the working solutions at the following concentrations: \nItracon... |
27,511 | Metastatic Tumors to the Jaw: A Retrospective Cross Sectional Study in Educational Hospitals and Institutions in Cairo Governorate. | null | dx.doi.org/10.17504/protocols.io.64xhgxn | null | Samar Ali Hasan, Dr. Manar Abdul Waniss Mohammed Abdul Aziz, Professor Dr. Mona Ezzat Wali | TITLE: Metastatic Tumors to the Jaw: A Retrospective Cross Sectional Study in Educational Hospitals and Institutions in Cairo Governorate.
AUTHORS: Samar Ali Hasan, Dr. Manar Abdul Waniss Mohammed Abdul Aziz, Professor Dr. Mona Ezzat Wali
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.icycaxw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
51,583 | Kelp DNA extractions - HMW | 4 | null | https://www.protocols.io/view/kelp-dna-extractions-hmw-bwk7pczn | wpearman | TITLE: Kelp DNA extractions - HMW
AUTHORS: wpearman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">DNA extraction protocol for Durvillaea antarctica with extremely high polysaccharide levels.</div></div>
[STEPS]
?. Add 500mg of freeze dried kelp powder to 7mls of lysis buffer (4% CTAB, 2% PVP 40... | ["Add 500mg of freeze dried kelp powder to 7mls of lysis buffer (4% CTAB, 2% PVP 40K, 80mM borax, 250mM EDTA, 20mM tris pH 8) and 20ul proteinase K.\nThis solution is extremely viscous, it couldn't pipette it - instead I poured it into a 15ml tube.", "Mix together and lyse overnight at 65 degrees", "Cool to room temper... |
95,762 | Protein Lysate Preparation Protocol (Culture) | 1 | dx.doi.org/10.17504/protocols.io.e6nvwdwd9lmk/v1 | https://www.protocols.io/view/protein-lysate-preparation-protocol-culture-c9rsz56e | Scott Vermilyea | TITLE: Protein Lysate Preparation Protocol (Culture)
AUTHORS: Scott Vermilyea
[DESCRIPTION]
This protocol details about the protein lysate of the culture.
[STEPS]
SECTION: Protein Lysate Preparation (Culture)
1. Wash cells using cold PBS followed by ice-cold 1x TNE lysis buffer containing 1% SDS, 0.5% NP-40, 0.5% D... | ["[Protein Lysate Preparation (Culture)] Wash cells using cold PBS followed by ice-cold 1x TNE lysis buffer containing 1% SDS, 0.5% NP-40, 0.5% DOC and protease/phosphatase inhibitors.\n\nTNE lysis buffer:", "[Protein Lysate Preparation (Culture)] Use cell scraper to remove all detached cells and keep on ice for 5 min.... |
88,992 | A protocol for co-designing a school-based healthy eating intervention for adolescents in urban Bangladesh | 4 | dx.doi.org/10.17504/protocols.io.kqdg3x87eg25/v1 | https://www.protocols.io/view/a-protocol-for-co-designing-a-school-based-healthy-c258yg9w | Nandeeta Samad, Lindsay Bearne, Fahmida Akter, Marc Delord, Divya Parmar | TITLE: A protocol for co-designing a school-based healthy eating intervention for adolescents in urban Bangladesh
AUTHORS: Nandeeta Samad, Lindsay Bearne, Fahmida Akter, Marc Delord, Divya Parmar
[DESCRIPTION]
Background
Unhealthy eating among adolescents can lead to an increased prevalence of overweight and obesity.... | ["[Recruiting stakeholders] Both internal and external stakeholder will be recruited. Internal stakeholders will be adolescents, teachers, and parents.", "[Recruiting stakeholders] For adolescents, the inclusion criteria will be aged between 10 and 19 years, studying at any of the selected secondary schools, and writte... |
null | null | null | dx.doi.org/10.17504/protocols.io.j5hcq36 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
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