id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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29,082 | Dural Cell Isolation and Culturing - Collection | 2 | dx.doi.org/10.17504/protocols.io.8m2hu8e | https://www.protocols.io/view/dural-cell-isolation-and-culturing-collection-8m2hu8e | Andrea Argouarch | TITLE: Dural Cell Isolation and Culturing - Collection
AUTHORS: Andrea Argouarch
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocols included in this collection describe cell isolation from human dura mater, culturing, and banking. </div></div>
[STEPS] | [] |
63,938 | Slim Fast Keto Reviews: Is Keto Pills Really Works? | 1 | dx.doi.org/10.17504/protocols.io.e6nvwkqp9vmk/v1 | https://www.protocols.io/view/slim-fast-keto-reviews-is-keto-pills-really-works-capasdie | slimfastketoreviewspills | TITLE: Slim Fast Keto Reviews: Is Keto Pills Really Works?
AUTHORS: slimfastketoreviewspills
[DESCRIPTION]
Slim Fast Keto Reviews: Is Keto Pills Really Works?
[STEPS]
SECTION: Slim Fast Keto Reviews: Is Keto Pills Really Works?
1. | ["[Slim Fast Keto Reviews: Is Keto Pills Really Works?]"] |
43,703 | Dot mutation | 1 | dx.doi.org/10.17504/protocols.io.bnwxmffn | https://www.protocols.io/view/dot-mutation-bnwxmffn | Zhujun Wei | TITLE: Dot mutation
AUTHORS: Zhujun Wei
[STEPS]
?. The entire plasmid was amplified reversely by PCR using primers with the fragment sequence that you want to replace.
?. The temple plasmids in the PCR process were digested by DpnI enzyme.
?. The digested product was transferred into DH5α. Overnight culture them at 37... | ["The entire plasmid was amplified reversely by PCR using primers with the fragment sequence that you want to replace.", "The temple plasmids in the PCR process were digested by DpnI enzyme.", "The digested product was transferred into DH5α. Overnight culture them at 37℃.", "To determine whether the vector was construc... |
78,529 | GUT MICROBIOME QUESTIONNAIRE | 1 | dx.doi.org/10.17504/protocols.io.8epv5jk26l1b/v1 | https://www.protocols.io/view/gut-microbiome-questionnaire-cqw9vxh6 | null | TITLE: GUT MICROBIOME QUESTIONNAIRE
AUTHORS:
[DESCRIPTION]
The protocol details the gut microbiome questionnaire_self adminsitered by subjects.
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.hw8b7hw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes the use of neutralizing antibodies to block receptrs for assessing involvement in bystander killing by BiTE®-activated T cells.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kjccuiw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Traditionally, social scientists perceived causality as regularity. As a consequence, qualitative comparative case study research was regarded as unsuitable for drawing causal inferences. Apparently, few cases cannot establish regularity. The dominant perception of causality ... | [] |
100,990 | Generation of Flp-In™ T-REx™ 293 cells stably expressing VPS13C^mClover under a tetracycline inducible promoter | 0 | dx.doi.org/10.17504/protocols.io.36wgqnnnxgk5/v1 | https://www.protocols.io/view/generation-of-flp-in-t-rex-293-cells-stably-expres-deu63eze | Xinbo Wang, Shujun Cai, Will Hancock-Cerutti, Pietro De Camilli | TITLE: Generation of Flp-In™ T-REx™ 293 cells stably expressing VPS13C^mClover under a tetracycline inducible promoter
AUTHORS: Xinbo Wang, Shujun Cai, Will Hancock-Cerutti, Pietro De Camilli
[DESCRIPTION]
This method describes the generation of Flp-In™ T-REx™ 293 cells stably expressing internally mClover tagged VPS1... | ["[Cloning] Clone gene of interest into pcDNA5/FRT/TO. For VPS13C^mClover the In-Fusion system (Takara Catalog # 638947) was used.", "[Cloning] Sequence plasmid.", "[Cell culture] Thaw and culture Flp-In™ T-REx™ 293 cells according to Gibco manual (Catalog # R780-07) in a 10cm dish. Once cells are 90% Confluent, split.... |
63,664 | LCMSMethods.org 2022 -- Dual Trap Proteomics Methods for TIMSTOF | 1 | dx.doi.org/10.17504/protocols.io.5qpvob27dl4o/v1 | https://www.protocols.io/view/lcmsmethods-org-2022-dual-trap-proteomics-methods-caeqsbdw | Simion Kreimer | TITLE: LCMSMethods.org 2022 -- Dual Trap Proteomics Methods for TIMSTOF
AUTHORS: Simion Kreimer
[DESCRIPTION]
Dual trap methods for TIMSTOF
[STEPS]
1. Protocol by Dr. Simion Kreimer of Cedar Sinai
| ["Protocol by Dr. Simion Kreimer of Cedar Sinai"] |
20,004 | U Mass - Bilirubin | null | dx.doi.org/10.17504/protocols.io.xscfnaw | null | Jason Kim | TITLE: U Mass - Bilirubin
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span><span style = "font-weight:bold;"> </span></div><div class = "text-block">This experiment involves a spectrophotometric measurement using Roche Cobas Clinical... | ["Perform daily quality control assessment of instrumentation before analysis.", "Load each sample into a specialized micro-sample cup for the clinical chemistry\nanalyzer.", "Select Bilirubin test on display and run the analysis.", "Collect and analyze the data."] |
74,292 | KASP genotyping | 4 | dx.doi.org/10.17504/protocols.io.kqdg39qr7g25/v1 | https://www.protocols.io/view/kasp-genotyping-cksuuwew | FishFloorUCL | TITLE: KASP genotyping
AUTHORS: FishFloorUCL
[DESCRIPTION]
KASP is a genotyping assay which you can use to differentiate wild types vs heterozygous vs homozygous larvae/finclips. It is well suited to genotype a SNP or a small CRISPR-generated indel. Once you confirmed it is working well, the assay is pretty fast to ru... | ["[Design the KASP primers] Eirinn Mackay, a Fish Floor alumnus (Wilson lab), created an online tool to create the KASP primer. This circumvents using the company's service, which charges ~ 60£ for a simple mix of three PCR primers.\n\nGo to\nhttps://kasp.eirinn.org/\n\nWe are assuming here that you already sequenced y... |
null | null | null | dx.doi.org/10.17504/protocols.io.ci2ugd | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
How to make a 50 mg/ml <a href="http://store.p212121.com/kanamycin-monosulfate/" target="_blank">Kanamycin</a> Stock Solution
[STEPS]
?.
?.
?.
?. | [] |
66,897 | Total Health ACV Keto Gummies | 1 | dx.doi.org/10.17504/protocols.io.j8nlkkq4dl5r/v1 | https://www.protocols.io/view/total-health-acv-keto-gummies-cdjrs4m6 | kzlwokvz | TITLE: Total Health ACV Keto Gummies
AUTHORS: kzlwokvz
[DESCRIPTION]
Total Health ACV Keto Gummies
[STEPS]
1. It can in like manner be trying to follow a low-carb diet plan, causing less accomplishment with a yo diet plan and keeping a sound weight. Assuming you attempt Total Health ACV Keto Gummies, you can bring ... | ["It can in like manner be trying to follow a low-carb diet plan, causing less accomplishment with a yo diet plan and keeping a sound weight. Assuming you attempt Total Health ACV Keto Gummies, you can bring down your gamble by picking lean meats, fish, and vegan protein sources, choosing low-fat dairy items, eating mo... |
28,959 | Aptamer 2-step conjugation protocol (EMD Adaptation) | null | dx.doi.org/10.17504/protocols.io.8h7ht9n | null | Jorge Fernández | TITLE: Aptamer 2-step conjugation protocol (EMD Adaptation)
AUTHORS: Jorge Fernández
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The following protocol consist on an aptamer adaptation from Merck for Antibody conjugation protocol to carboxyl modified microparticles.</div></div>
[STEPS]
?. [Reac... | ["[Reactives Preparation]\nPrepare a 200 mM aqueous solution of sulfo-NHS. Weight 22.1 mg of sulfo-NHS and dissolve it in 500 μL of distilled water. Keep stored at 4 ºC. Sulfo-NHS can be stored under refrigerator for a couple of months.", "[Reactives Preparation]\nPrepare 200mM EDC solution. Pipette 22 μL of 97% EDC in... |
41,904 | High-throughput SARS-CoV-2 RNA extraction from nasopharyngeal swabs using repurposed 3D printers v2. | 4 | null | https://www.protocols.io/view/high-throughput-sars-cov-2-rna-extraction-from-nas-bk6qkzdw | Koen Vandelannoote | TITLE: High-throughput SARS-CoV-2 RNA extraction from nasopharyngeal swabs using repurposed 3D printers v2.
AUTHORS: Koen Vandelannoote
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Here, we describe a semi-automated workflow for paramagnetic bead-based extraction of SARS-CoV-2 RNA from hum... | ["[Pre-process sample information]\nScan the barcodes of 91 nasopharyngeal swabs using a barcode scanner and the \"20200908_Ender_VX-500_extraction_template.xlsx\" spreadsheet. Populate the “SampleID” column of the “Sample log” sheetRandomly include a negative extraction control for every set of 24 swabs in the sheet. ... |
90,290 | Cell culture | 1 | dx.doi.org/10.17504/protocols.io.36wgq3bk5lk5/v1 | https://www.protocols.io/view/cell-culture-c4esytee | Alexander Röntgen | TITLE: Cell culture
AUTHORS: Alexander Röntgen
[DESCRIPTION]
Protocol to culture SH-SY5Y cells.
[STEPS]
SECTION: Cell culture
1. Culture human SH-SH5Y cells at 37 °C and 5% CO2
SECTION: Cell culture
2. Split cells at 80–90% confluency
SECTION: Cell culture
2.1. Discard medium, rinse with PBS
SECTION: Cell culture
2.... | ["[Cell culture] Culture human SH-SH5Y cells at 37 °C and 5% CO2", "[Cell culture] Split cells at 80–90% confluency", "[Cell culture] Discard medium, rinse with PBS", "[Cell culture] Incubate cells with Trypsin-EDTA for 5 min at 37 °C and 5% CO2", "[Cell culture] Add 9 mL medium to neutralise Trypsin-EDTA", "[Cell cult... |
21,202 | Coding Manual for Childhood Obesity-Relevant Policy Documents | null | dx.doi.org/10.17504/protocols.io.yxsfxne | null | Itzhak Yanovitzky, Matthew S. Weber | TITLE: Coding Manual for Childhood Obesity-Relevant Policy Documents
AUTHORS: Itzhak Yanovitzky, Matthew S. Weber
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">This is a new version of the protocol - after doing our study, we found that codes x through y didn't re... | [] |
39,305 | PROTOCOL FOR: An optimized procedure for quantitative analysis of mitophagy with the mtKeima system using flow cytometry | 4 | dx.doi.org/10.17504/protocols.io.bimhkc36 | https://www.protocols.io/view/protocol-for-an-optimized-procedure-for-quantitati-bimhkc36 | Nathaniel J Winsor, Samuel A Killackey, Dana J Philpott, Stephen E Girardin | TITLE: PROTOCOL FOR: An optimized procedure for quantitative analysis of mitophagy with the mtKeima system using flow cytometry
AUTHORS: Nathaniel J Winsor, Samuel A Killackey, Dana J Philpott, Stephen E Girardin
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Abst... | ["[Cell transfection and staining:]\nPlate 1 x 106 wild-type (WT) HEK293T cells per 6 well plate overnight.\nHINT: As mitophagic stimuli can decreases cell viability, we recommend pooling a minimum of two wells (2 x 106cells) per condition to ensure adequate cell numbers.", "[Cell transfection and staining:]\nTransfect... |
24,879 | Akta Pure General Protocol | null | dx.doi.org/10.17504/protocols.io.4ipgudn | null | Annie Kwon | TITLE: Akta Pure General Protocol
AUTHORS: Annie Kwon
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A general protocol for running and cleaning the Akta Pure. Always refer to manuals for specific columns before a run for specific details regarding a column's pressure limits, capatability with spec... | ["[Pump wash]\nPerform a pump wash (and/or system wash) with buffers in reverse order of how they will be used. For example, if you intend to run a sequence of 1) A1-buffer1, 2) B1-buffer2, you will want to run a pump wash (and/or system wash) for B1, then A1.", "[Prime and fill sample loop]\nFill a syringe with at lea... |
53,485 | Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360 | 1 | dx.doi.org/10.17504/protocols.io.bygmptu6 | https://www.protocols.io/view/mapping-cgrp-ir-innervation-of-male-mice-stomach-w-bygmptu6 | Duyen Nguyen, Jichao Ma | TITLE: Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360
AUTHORS: Duyen Nguyen, Jichao Ma
[DESCRIPTION]
This protocol describes the process of using Neurolucida 360 software to map the topographical organization of Calcitonin gene related peptide – immunoreactive axons and terminals in the muscu... | ["[Animals] Male C57BL/6J mice (The Jackson laboratory), n= 8, age 12-16 weeks were used in this study. Animals were kept in the animal room at which the dark/light cycle is set to 12/12 hours and water and food were supplied ad libitum. All procedures were carried out under the ethical guidelines of University of Cent... |
106,621 | PFF seeding in iPSC-derived cortical neurons | 0 | dx.doi.org/10.17504/protocols.io.ewov19k87lr2/v1 | https://www.protocols.io/view/pff-seeding-in-ipsc-derived-cortical-neurons-dkc54sy6 | Isabel Lam, Alain Ndayisaba, Vikram Khurana | TITLE: PFF seeding in iPSC-derived cortical neurons
AUTHORS: Isabel Lam, Alain Ndayisaba, Vikram Khurana
[DESCRIPTION]
PFF seeding in iPSC-derived cortical neurons_reformatted
[STEPS] | [] |
57,548 | New test WS | 1 | dx.doi.org/10.17504/protocols.io.b4fkqtkw | https://www.protocols.io/view/new-test-ws-b4fkqtkw | Bogdan Bogdanov | TITLE: New test WS
AUTHORS: Bogdan Bogdanov
[DESCRIPTION]
A wonderful serenity has taken possession of my entire soul, like these sweet mornings of spring which I enjoy with my whole heart. I am alone, and feel the charm of existence in this spot, which was created for the bliss of souls like mine. I am so happy, my ... | ["Sed ut perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo. Nemo enim ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit, sed quia consequuntur magn... |
80,503 | Protocol for Generating Stably Expressed Mammalian Cell Lines Using Lentivirus | 4 | dx.doi.org/10.17504/protocols.io.14egn2qo6g5d/v1 | https://www.protocols.io/view/protocol-for-generating-stably-expressed-mammalian-csuxwexn | creative-biogene | TITLE: Protocol for Generating Stably Expressed Mammalian Cell Lines Using Lentivirus
AUTHORS: creative-biogene
[DESCRIPTION]
Stable cell lines generated using lentivirus exhibit long term protein expression and the system is highly reproducible. In this protocol, we describe the use of the 3rd generation lentiviral s... | ["[Experiment Summary] Stable cell lines generated using lentivirus exhibit long term protein expression and the system is highly reproducible. In this protocol, we describe the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines. First plasmid contains your ... |
null | null | null | dx.doi.org/10.17504/protocols.io.gqebvte | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<div>
<div>
<div class="b-start-block editable-content">
<p>GreenGlo ™, 20,000X in Water, is a non-carcinogenic and non-toxic alternative to Ethidium bromide used for the detection of nucleic acids in agarose gels. It is as sensitive as Ethidium bromide. There is no toxic ... | [] |
65,204 | C4 ZipTip Solid Phase Extraction | 1 | null | https://www.protocols.io/view/c4-ziptip-solid-phase-extraction-cbwuspew | Lauren Adams | TITLE: C4 ZipTip Solid Phase Extraction
AUTHORS: Lauren Adams
[DESCRIPTION]
Solid phase extraction for clean-up and concentration of proteins prior to introduction into the mass spectrometer.
[STEPS]
1. Activate Ziptip by pipetting 10 µL of C4 ZipTip Activation Buffer and discarding onto a Kimwipe for a total of 6 ... | ["Activate Ziptip by pipetting 10 µL of C4 ZipTip Activation Buffer and discarding onto a Kimwipe for a total of 6 times.", "Equilibrate the Ziptip by pipetting 10 µL of C4 ZipTip Equilibration/Wash Buffer and discarding onto a Kimwipe for a total of 6 times.", "Remove C4 Ziptip from p20 pipette and place safely back i... |
66,632 | https://www.facebook.com/Tiger-Woods-CBD-Gummies-106352182147664/ | 1 | dx.doi.org/10.17504/protocols.io.kxygxzb4wv8j/v1 | https://www.protocols.io/view/https-www-facebook-com-tiger-woods-cbd-gummies-106-cdbgs2jw | fddaaad | TITLE: https://www.facebook.com/Tiger-Woods-CBD-Gummies-106352182147664/
AUTHORS: fddaaad
[DESCRIPTION]
Among the few tricks and CBD Gummies things, the Tiger Woods CBD Gummies US is the exceptional recipe made to give you the expected lightening with hazard free intensity. You can carry on with an aggravation fre... | ["Tiger Woods CBD Gummies\n==❱❱ Huge Discounts: [HURRY UP ] Absolute Tiger Woods CBD Gummies (Available) Order Online Only!! ❰❰=\n \n\nTiger Woods CBD Gummies - You plan to recuperate just as truly feel over and above anyone's expectations previously! However when you are encountering steady medical conditions, it tend... |
null | null | null | dx.doi.org/10.17504/protocols.io.hpbb5in | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
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?.
?. | [] |
71,689 | Lectin C gene analysis | 1 | dx.doi.org/10.17504/protocols.io.3byl4jb32lo5/v1 | https://www.protocols.io/view/lectin-c-gene-analysis-ch9ht936 | Tran Vinh Phuong, Nguyen Ngoc Phuoc | TITLE: Lectin C gene analysis
AUTHORS: Tran Vinh Phuong, Nguyen Ngoc Phuoc
[DESCRIPTION]
Mammalian Tissue Total RNA Purification Protocol by GeneJET RNA Purification Kit (Thermo Scientific, USA)
Before starting:
• Supplement the required amount of Lysis Buffer with β-mercaptoethanol or DTT. Add 20 μL of 14.3 M β-merc... | ["Mammalian Tissue Total RNA Purification Protocol by GeneJET RNA Purification Kit (Thermo Scientific, USA)", "First Stra nd cDNA Synthesis by RevertAid First Strand cDNA Synthesis kit (Thermo Fisher Scientific, USA)", "PCR products purification by GeneJET Gel Extraction kit (Thermo Scientific, USA)", "Cloning with pG... |
null | null | null | dx.doi.org/10.17504/protocols.io.gtvbwn6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Stock solution for 50x TAE. TAE buffer is a solution made up of Tris base, acetic acid and EDTA (Tris-acetate-EDTA). It is a common buffer for DNA separation using standard agarose gel electrophoresis.</p>
[STEPS]
?.
?.
?.
?. | [] |
61,472 | Shipping protocols | 4 | dx.doi.org/10.17504/protocols.io.bp2l617edvqe/v1 | https://www.protocols.io/view/shipping-protocols-b798rr9w | Gabriel J J. Barrero, Abraham Palmer, Oksana Polesskaya | TITLE: Shipping protocols
AUTHORS: Gabriel J J. Barrero, Abraham Palmer, Oksana Polesskaya
[DESCRIPTION]
Shipping tissue protocol
[STEPS]
SECTION: Collection
1. Collection
SECTION: Collection
1.1. Put the spleen into a pre-labeled 2 mLmicrocentrifuge tube (for example, Fisher Scientific cat#05-408-138). Add appr... | ["[Collection] Collection", "[Collection] Put the spleen into a pre-labeled 2 mLmicrocentrifuge tube (for example, Fisher Scientific cat#05-408-138). Add approximately0.5 mLsaline solution in the tube with the spleen to prevent dry-freezing of tissue, but make sure that the tube is not over-filled.\n If your rats have ... |
null | null | null | dx.doi.org/10.17504/protocols.io.ejsbcne | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
A brief protocol for the preparation of glyoxal used in glyoxal/borate and RNase T1-based detection of inosine residues in RNAs.
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ch3t8m | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
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null | null | null | dx.doi.org/10.17504/protocols.io.kfnctme | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describe how to asess secretion efficiency of diferrent constructs in <em>Chalmydomonas reinhardtii</em>, using a fluorescent protein. Colonies are picked from transformation plate and inoculated on TAP media for growth. A high number of colonies is picked to av... | [] |
87,577 | Protocols for "Mitochondrial Anchored Protein Ligase MAPL is an inflammatory rheostat that regulates immune signalling and cell death" | 2 | dx.doi.org/10.17504/protocols.io.8epv5x266g1b/v1 | https://www.protocols.io/view/protocols-for-34-mitochondrial-anchored-protein-li-czrzx576 | mai.nguyen, Jack Collier, Olesia Ignatenko, Sidong Huang, Michel Desjardins, heidi.mcbride | TITLE: Protocols for "Mitochondrial Anchored Protein Ligase MAPL is an inflammatory rheostat that regulates immune signalling and cell death"
AUTHORS: mai.nguyen, Jack Collier, Olesia Ignatenko, Sidong Huang, Michel Desjardins, heidi.mcbride
[DESCRIPTION]
Cell death is inhibited in cancers but increased in neu... | [] |
31,113 | Extraction of Oxycodone from Plasma for Mass Spec Analysis | 1 | null | https://www.protocols.io/view/extraction-of-oxycodone-from-plasma-for-mass-spec-bamhic36 | Sierra Simpson, Olivier George | TITLE: Extraction of Oxycodone from Plasma for Mass Spec Analysis
AUTHORS: Sierra Simpson, Olivier George
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">From the Momper Lab.</div></div>
[STEPS]
?. [Harvesting Blood ]
Collect blood in a tube coated with EDTA. Heparin leads to hemolysis which can ef... | ["[Harvesting Blood ]\nCollect blood in a tube coated with EDTA. Heparin leads to hemolysis which can effect downstream processing.", "[Harvesting Blood ]\nSpin blood at 3000rpm for 10 minutes to separate erythrocytes from plasma", "[Harvesting Blood ]\nAliquot plasma into 50-100ul aliquits to avoid freeze thaw. 20ul i... |
96,323 | Yeast gDNA isolation | 0 | dx.doi.org/10.17504/protocols.io.eq2lyjm7elx9/v1 | https://www.protocols.io/view/yeast-gdna-isolation-dabb2ain | is Sparrow | TITLE: Yeast gDNA isolation
AUTHORS: is Sparrow
[DESCRIPTION]
This protocol is based on https://cshprotocols.cshlp.org/content/2020/10/pdb.prot098152.full doi:10.1101/pdb.prot098152
The protocol details how to perform a small scale yeast gDNA isolation including a proteinase K incubation step to visualize linear cyt... | ["[DNA extraction] Start a 10 mL yeast culture and grow to saturation overnight or over 2 days\n\nNote: for p1_wt (but not p1_rec) containing strains, p1 can be lost if grown in YPD for prolonged periods of time (based on my estimations, 2 or 3 1:1000 passages). Be mindful of this.", "[DNA extraction] Aliquot 5 mL of c... |
null | null | null | dx.doi.org/10.17504/protocols.io.qijducn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Project Summary </strong></p>
<p><strong>Background Rationale </strong></p>
<p>Neonatal hypoglycaemia is a common condition, particularly affecting LBW newborns, and is associated with both acute and long standing complications. Ideally, the mother can provide breast ... | [] |
23,205 | Striga hermonthica germination assay | null | dx.doi.org/10.17504/protocols.io.2wdgfa6 | null | Emily Bellis, Elizabeth Kelly | TITLE: Striga hermonthica germination assay
AUTHORS: Emily Bellis, Elizabeth Kelly
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes a standard germination assay for </span><span style = "font-style:italic;">Striga hermonthica</span><span> carried out in the Penn State qu... | ["[Surface sterilization]\nTesting seeds from populations:Scoop appropriate amount of seeds into a sterile 15 mL conical vial. We make 'scoops' out of PCR tubes and have found that one 'scoop' filled almost to the top of the tube is approximately 1800 seeds.\nDo this on a Monday, so that all subsequent steps (adding ho... |
36,619 | 2100 Bioanalyzer Operation for PCR, GTP and IVT Products | null | dx.doi.org/10.17504/protocols.io.bfzjjp4n | https://www.protocols.io/view/2100-bioanalyzer-operation-for-pcr-gtp-and-ivt-pro-bfzjjp4n | Allen Institute for Brain Science | TITLE: 2100 Bioanalyzer Operation for PCR, GTP and IVT Products
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes bioanalyzer operation. The Agilent Bioanalyzer is used as a quality control step for PCR and IVT products. Peak size, sh... | [] |
87,715 | Magnetic bead cleaning of PCR products with Cytiva Sera-Mag Select | 1 | dx.doi.org/10.17504/protocols.io.n2bvj393wlk5/v1 | https://www.protocols.io/view/magnetic-bead-cleaning-of-pcr-products-with-cytiva-czwbx7an | Alexander Eiler, Even Garvang | TITLE: Magnetic bead cleaning of PCR products with Cytiva Sera-Mag Select
AUTHORS: Alexander Eiler, Even Garvang
[DESCRIPTION]
Magnetic bead cleaning of PCR products, adapted for Cytiva Sera-Mag Select beads. Forked from an older protocol using AMPure XP beads with minimal changes.
[GUIDELINES]
Work at post-PCR lab.
... | ["Calculate the volume needed for the beads. For normal length primers a bead:sample ratio of 0.8:1 is likely OK. For long primers you can use a ratio of down to 0.65:1 (probably even lower). If you can still see primer dimers on a gel after cleanup, try a lower bead:sample ratio.\n\n0.8 * sample volume =", "Shake the ... |
91,105 | W-3 WATER STORAGE | 4 | dx.doi.org/10.17504/protocols.io.8epv5xm96g1b/v1 | https://www.protocols.io/view/w-3-water-storage-c479yzr6 | REDI-NET Consortium | TITLE: W-3 WATER STORAGE
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol describes leech storage.
[GUIDELINES]
OBJECTIVE
To outline steps for properly storing field-collected leech samples and nucleic acid samples purified from these soil.
SUMMARY/SCOPE
The overarching aim of the REDI-NET is to develop a ... | ["[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Each collected water sample will be given a unique ID.", "[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Each water sample filtered membrane will be moved into an individual 60 mm Petri dish labeled with its original sample ID.", "[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Follow the ... |
39,121 | Protocol for RPA-PCR Couple | 3 | dx.doi.org/10.17504/protocols.io.bifrkbm6 | https://www.protocols.io/view/protocol-for-rpa-pcr-couple-bifrkbm6 | Mustafa Munawar | TITLE: Protocol for RPA-PCR Couple
AUTHORS: Mustafa Munawar
[STEPS] | [] |
65,246 | Keto Blast Gummy Bears Reviews[ Shark Tank] Read Shoking Scams | 3 | dx.doi.org/10.17504/protocols.io.x54v9ybpzg3e/v1 | https://www.protocols.io/view/keto-blast-gummy-bears-reviews-shark-tank-read-sho-cbx6spre | Keto Blast Gummies | TITLE: Keto Blast Gummy Bears Reviews[ Shark Tank] Read Shoking Scams
AUTHORS: Keto Blast Gummies
[DESCRIPTION]
You have tried to lose weight but the scales won’t move no matter what. What can a girl do? These keto blast gummies are the answer! They are made from all-natural ingredients, and contain only 0.5g sugar p... | [] |
40,968 | Suggested field procedures for collecting soil/litter arthropods in a tropical rainforest for long-term monitoring with DNA metabarcoding | 3 | dx.doi.org/10.17504/protocols.io.bj9gkr3w | https://www.protocols.io/view/suggested-field-procedures-for-collecting-soil-lit-bj9gkr3w | Yves Basset | TITLE: Suggested field procedures for collecting soil/litter arthropods in a tropical rainforest for long-term monitoring with DNA metabarcoding
AUTHORS: Yves Basset
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Suggested field procedures for collecting soil/litte... | [] |
95,415 | Protocol name | 1 | null | https://www.protocols.io/view/protocol-name-c9exz3fn | Joanna Sendecka, Joanna Sendecka | TITLE: Protocol name
AUTHORS: Joanna Sendecka, Joanna Sendecka
[DESCRIPTION]
This is a test protocol created for the purpose of developing guidelines.
[STEPS]
SECTION: Section one
1. Here we start writing the protocol. It will last 10 min
SECTION: Section one
1.1. and here we continue
SECTION: Section one
2. We giv... | ["[Section one] Here we start writing the protocol. It will last 10 min", "[Section one] and here we continue", "[Section one] We give examples of amounts e.g. volume 0.5 L or weight 200 g or voltage 3 V", "[Section one]"] |
106,624 | Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates | 0 | dx.doi.org/10.17504/protocols.io.bp2l62enzgqe/v1 | https://www.protocols.io/view/seeded-amplification-assay-saa-from-neuronal-cell-dkc84szw | Isabel Lam, Alain Ndayisaba, Vikram Khurana | TITLE: Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates
AUTHORS: Isabel Lam, Alain Ndayisaba, Vikram Khurana
[DESCRIPTION]
Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ci5ug5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
How to make a 25-50 mg/ml Chloramphenicol Stock Solution
[STEPS]
?.
?. | [] |
103,747 | FUNDIS Fungal Tissue Sampling for PCR | 0 | null | https://www.protocols.io/view/fundis-fungal-tissue-sampling-for-pcr-dhjb34in | Harte Singer | TITLE: FUNDIS Fungal Tissue Sampling for PCR
AUTHORS: Harte Singer
[DESCRIPTION]
How to sample dried fungi for PCR amplification
[STEPS]
SECTION: Tissue Sampling
9. Find a suitable place to conduct tissue sampling. A hard, nonporous surface that can be wiped clean with rubbing alcohol is ideal. Turn off any fans and ... | ["[Tissue Sampling] Find a suitable place to conduct tissue sampling. A hard, nonporous surface that can be wiped clean with rubbing alcohol is ideal. Turn off any fans and close any windows so there is minimal air movement. Wash your hands and gather your materials and mushrooms for sampling.", "[Tissue Sampling] Set ... |
100,419 | Acetaldehyde Quantification in Microbial Fermentation Samples | 0 | dx.doi.org/10.17504/protocols.io.4r3l2q294l1y/v1 | https://www.protocols.io/view/acetaldehyde-quantification-in-microbial-fermentat-debb3ain | Isaiah D Richardson, Bishaldsharmath, Daniel G Olson | TITLE: Acetaldehyde Quantification in Microbial Fermentation Samples
AUTHORS: Isaiah D Richardson, Bishaldsharmath, Daniel G Olson
[DESCRIPTION]
This protocol details a broadly applicable approach for quantifying the concentration of acetaldehyde present in a microbial fermentation sample. To quantify acetaldehyde, a ... | ["[Preparation of the Derivatization Reagent] The primary objective of this section of the protocol is to prepare a 100 mL stock of derivatization reagent, which consists of 0.9 g/L 2,4-DNPH and 1.0% phosphoric acid (H3PO4) in acetonitrile.", "[Preparation of the Derivatization Reagent] Carefully add the 0.09 g of 2,4-... |
33,861 | Isolation of the Antioxidant Metabolite from the EAF | 1 | dx.doi.org/10.17504/protocols.io.bdbdi2i6 | https://www.protocols.io/view/isolation-of-the-antioxidant-metabolite-from-the-e-bdbdi2i6 | Jing Xu | TITLE: Isolation of the Antioxidant Metabolite from the EAF
AUTHORS: Jing Xu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>EAF (4.13 g), which showed the most antioxidantactivity, was subjected to column chromatography on a silica gel column chromatography (CC), employing a step gradient of ... | [] |
36,587 | Glycine-Sodium Hydroxide Buffer | null | dx.doi.org/10.17504/protocols.io.bfyjjpun | https://www.protocols.io/view/glycine-sodium-hydroxide-buffer-bfyjjpun | Neilier Junior | TITLE: Glycine-Sodium Hydroxide Buffer
AUTHORS: Neilier Junior
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors... | ["[Glycine-Sodium Hydroxide Buffer]\nMix and indicated volume of sodium hydroxide solutions. ABCDEFGH1mL of Sodium hydroxide4.08.816.827.232.038.645.52pH8.69.09.49.810.010.410.6\npH: to (a) 0.1 M Glycine; 7.5 g L-1 (M.W.: 75.0 g mol-1)(b) 0.1 M Sodium hydroxide; 4.0 g L-1 (M.W.: 40.0 g mol-1)\n[glycine]\nABCDEFGH1... |
null | null | null | dx.doi.org/10.17504/protocols.io.dfe3jd | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
Prepare wash buffer at same time as setting up hybridization.
[GUIDELINES]
<em><strong>Sample Collection</strong></em><br /><br /><em>Biosphere 2 Samples</em><br /><br />Materials<br />• Acid-Washed Glass Bottles (Enough for samples + 1 for collecting ocean water)<br />• 16% F... | [] |
83,156 | Rotarod Test to assess motor coordination in a mouse parkinsonian model | 1 | dx.doi.org/10.17504/protocols.io.kxygx3kwdg8j/v1 | https://www.protocols.click/view/rotarod-test-to-assess-motor-coordination-in-a-mou-cvfuw3nw | natalia.lopezgonzalezdelrey, Zachary Gaertner | TITLE: Rotarod Test to assess motor coordination in a mouse parkinsonian model
AUTHORS: natalia.lopezgonzalezdelrey, Zachary Gaertner
[DESCRIPTION]
Protocol for the evaluation of motor coordination in the rotarod assay in parkinsonian mice.
[STEPS]
SECTION: Open Field Test
1. Total duration: 4 days
SECTION: Training ... | ["[Open Field Test] Total duration: 4 days", "[Training phase] Duration: 3 days", "[Protocol] Training Phase: 3 days\n\nTesting Phase: 3 days", "[Training phase] Four trials separated by at least 30 min inter-trial intervals at constant velocity.", "[Test phase] Duration: 3 days", "[Test phase] Four trials separated by... |
77,111 | A scoping review of the impact of acquired communication impairments on sexuality, intimacy, and sexual health | 1 | null | https://www.protocols.io/view/a-scoping-review-of-the-impact-of-acquired-communi-cpixvkfn | Laura L Wolford, Nicholas Behn, Vanessa Arattia, Emma Power | TITLE: A scoping review of the impact of acquired communication impairments on sexuality, intimacy, and sexual health
AUTHORS: Laura L Wolford, Nicholas Behn, Vanessa Arattia, Emma Power
[DESCRIPTION]
Background
Communication is a crucial part of sexuality. Communication ability has been linked to ability to participa... | [] |
27,036 | Comments 1 | null | dx.doi.org/10.17504/protocols.io.6m4hc8w | null | Anna Ivanova | TITLE: Comments 1
AUTHORS: Anna Ivanova
[STEPS]
?. Rendered her for put improved concerns his. Ladies bed wisdom theirs mrs men months set. Everything so dispatched as it increasing pianoforte. Hearing now saw perhaps minutes herself his. Of instantly excellent therefore difficult he northward. Joy green but least mar... | ["Rendered her for put improved concerns his. Ladies bed wisdom theirs mrs men months set. Everything so dispatched as it increasing pianoforte. Hearing now saw perhaps minutes herself his. Of instantly excellent therefore difficult he northward. Joy green but least marry rapid quiet but. Way devonshire introduced expr... |
null | null | null | dx.doi.org/10.17504/protocols.io.g4rbyv6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Shows how to create a BLAST db using CyVerse. This example creates a database from E. coli's genome due to size constraints. This protocol is intended to demonstrate the methodology to creat the db but will not have any practicality since the db is only of E. coli's genome. <... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.md9c296 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Plasma FABP4 concentrations were analyzed blinded to their preeclampsia status with BioVendor Human AFABP ELISA (Cat. No: RD191036200R, Biovendor, Modrice, Czech Republic) in our study. The manufacturer reports a normal range of 19.58 ± 16.32 ng/mL (mean ± 2 SD) for 35- to 52... | [] |
71,546 | Quick DNA Extraction for Fungal Barcoding (X-Amp) | 4 | dx.doi.org/10.17504/protocols.io.4r3l2oy2pv1y/v5 | https://www.protocols.io/view/quick-dna-extraction-for-fungal-barcoding-x-amp-ch42t8ye | Stephen Douglas Russell | TITLE: Quick DNA Extraction for Fungal Barcoding (X-Amp)
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
Below you will find a simple and fast two-step DNA extraction protocol that works well with many different groups of fungi. This protocol can be utilized in preparation for DNA barcoding of fungi utilizing Oxf... | ["[Initial Preparation] Begin by laying out (96) 0.2mL cells on a 96-well PCR plate rack. This can be done in a plate or strip format. \n\nEven though it costs more, I prefer to use 8-strips with individual caps as they are easiest to work with. Only a single sample cell needs to be open at a time, individual rows can ... |
null | null | null | dx.doi.org/10.17504/protocols.io.sipecdn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>These protocols refer to a study having the aim to identify and test at field artificial structures suitable as collectors for common cuttlefish <em>Sepia officinalis</em> eggs in wild condition for restocking purpose.</p>
<p>During the same study, three different protocols w... | [] |
54,111 | RiboTag Immunoprecipitation | 4 | dx.doi.org/10.17504/protocols.io.by37pyrn | https://www.protocols.io/view/ribotag-immunoprecipitation-by37pyrn | Benjamin Hobson, Peter Sims | TITLE: RiboTag Immunoprecipitation
AUTHORS: Benjamin Hobson, Peter Sims
[DESCRIPTION]
This protocol describes an optimized method for RiboTag Immunoprecipitation from mouse brain tissue.
[BEFORE_START]
For tissue dissection, chill the brain matrix and dissection buffer for at least 30 minutes on ice.
For RiboTag ... | ["[Tissue Dissection] From the moment of euthanasia onward, changes in translation can occur rapidly. The dissection procedure should be conducted on ice and as quickly as possible. The following steps are suitable for mouse brain tissue; modification may be required for other tissues.", "[RiboTag Immunoprecipitation (... |
85,855 | DNA/RNA extraction from fresh-frozen tissue, AllPrep DNA/RNA/miRNA Universal Kit | 4 | dx.doi.org/10.17504/protocols.io.kxygx3mrwg8j/v3 | https://www.protocols.io/view/dna-rna-extraction-from-fresh-frozen-tissue-allpre-cx37xqrn | Annika Fendler | TITLE: DNA/RNA extraction from fresh-frozen tissue, AllPrep DNA/RNA/miRNA Universal Kit
AUTHORS: Annika Fendler
[DESCRIPTION]
Protocol for combined RNA and DNA extraction from fresh-frozen tissue using the AllPrep DNA/RNA/miRNA Universal Kit.
[BEFORE_START]
Preparations:
FRN buffer: Add 42 ml Isoprop to new bottle
RP... | ["[Tissue preparation] This protocol is for", "[Tissue preparation] Transfer tissue in 350 µL 600 µL RLT + ß-ME in 2 ml DNA LoBind tube.", "[Tissue preparation] Add a 5mm bead to each tube and lyse tissue in TissueLyser for 2 min @ 20 Hz", "[Tissue preparation] Spin down for 1 min @ 9000 x g", "[Tissue preparation] ... |
98,840 | Crystallization of Enterovirus D68 3C protease | 1 | dx.doi.org/10.17504/protocols.io.5qpvoky29l4o/v1 | https://www.protocols.io/view/crystallization-of-enterovirus-d68-3c-protease-dcry2v7w | ryan Lithgo, Peter Marples, Lizbé Koekemoer, Daren Fearon | TITLE: Crystallization of Enterovirus D68 3C protease
AUTHORS: ryan Lithgo, Peter Marples, Lizbé Koekemoer, Daren Fearon
[DESCRIPTION]
The development of effective broad-spectrum antivirals forms an important part of preparing for future pandemics. A cause for concern is the currently emerging pathogen Enterovirus D68... | ["[Crystallization experiment] Protein and buffer requirements:\n14.4 µL35 mg/mL \n2.88 mL \n7.2 µL seeds, dilution 1:1 000 000", "[Crystallization experiment] Dispense 30 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.\nDispense 50 286135 mg/mL to each lens using the SPT mosquito.\n... |
50,738 | 10X Genomics Single-Nucleus Assay of Transposase Accessible Chromatin-Sequencing for Epigenetic Profiling of Adult Human Tissues | 1 | dx.doi.org/10.17504/protocols.io.bvssn6ee | https://www.protocols.io/view/10x-genomics-single-nucleus-assay-of-transposase-a-bvssn6ee | Blue Lake, Dinh Diep, Kun Zhang, Sarah Urata | TITLE: 10X Genomics Single-Nucleus Assay of Transposase Accessible Chromatin-Sequencing for Epigenetic Profiling of Adult Human Tissues
AUTHORS: Blue Lake, Dinh Diep, Kun Zhang, Sarah Urata
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">10x Genomics Single Cell ATAC (v1) sequencing is a microdrople... | ["[Isolate Nuclei]\nPrepare nuclei according to the protocol \"Isolation of single nuclei from solid tissues\" steps 1-14. dx.doi.org/10.17504/protocols.io.ufketkw", "[Isolate Nuclei]\nResuspend nuclei in to of 1X Diluted Nuclei Buffer (volume depends on target concentration)\n100 µl\n1 mL", "[Isolate Nuclei]\nCount ... |
95,997 | Fish eRNA: water sampling and filtration through Sterivex filter unit | 4 | dx.doi.org/10.17504/protocols.io.j8nlkozrxv5r/v1 | https://www.protocols.io/view/fish-erna-water-sampling-and-filtration-through-st-c9y5z7y6 | Marine Vautier | TITLE: Fish eRNA: water sampling and filtration through Sterivex filter unit
AUTHORS: Marine Vautier
[DESCRIPTION]
The objective of this protocol is the sampling and the filtration of water samples through 0.45 µm Sterivex™ filter units with the necessary precautions for environmental RNA (eRNA) analysis.
This pro... | ["[WATER SAMPLING] Wear gloves and open the bottle without touching the inside of the cap or the neck of the bottle.\n\nSeveral options are available:\n\n-Surface sampling :Collect by hand sub-surface water (10-20 cm below the water surface) and close the bottle carefully. \n\n-Depth sampling : If water is collected d... |
36,236 | Group learning protocol | 1 | null | https://www.protocols.io/view/group-learning-protocol-bfmkjk4w | Wolfram Moebius | TITLE: Group learning protocol
AUTHORS: Wolfram Moebius
[DESCRIPTION]
Group learning protocol
[STEPS]
SECTION: Making media
1. 400 µL of total volume
200 µL
200 µL
SECTION: Analysing data
2.
SECTION: Analysing data
3. | ["[Making media] 400 µL of total volume\n200 µL \n200 µL", "[Analysing data]", "[Analysing data]"] |
51,907 | Total nucleic acids extraction, purification and cDNA synthesis from soil | 2 | dx.doi.org/10.17504/protocols.io.bwxbpfin | https://www.protocols.io/view/total-nucleic-acids-extraction-purification-and-cd-bwxbpfin | Roey Angel | TITLE: Total nucleic acids extraction, purification and cDNA synthesis from soil
AUTHORS: Roey Angel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Extraction of DNA, and especially RNA from soils, can be challenging due to the presence of organic impurities, which can inhibit downstream enzymatic ... | [] |
38,405 | Meta-analysis of scope harvesting radial artery | 1 | dx.doi.org/10.17504/protocols.io.bhrdj526 | https://www.protocols.io/view/meta-analysis-of-scope-harvesting-radial-artery-bhrdj526 | Tzu-yen Huang | TITLE: Meta-analysis of scope harvesting radial artery
AUTHORS: Tzu-yen Huang
[STEPS]
?. Check MeSH terms, set reaserch keywords: radial artery and harvest.
?. Searches in Pubmed, Medline, the Cochrane Library, and EMBASE, to find studies published from January 1974 to July 2019.
?. Articles with comparisons of open a... | ["Check MeSH terms, set reaserch keywords: radial artery and harvest.", "Searches in Pubmed, Medline, the Cochrane Library, and EMBASE, to find studies published from January 1974 to July 2019.", "Articles with comparisons of open and endoscope artery harvesting were included as long as there were adult patients with c... |
101,969 | BAF_Protocol_013_Lipidomics: Database Search MS-DIAL and Analysis using Metaboanalyst 6.0 | 0 | dx.doi.org/10.17504/protocols.io.ewov192jklr2/v1 | https://www.protocols.io/view/baf-protocol-013-lipidomics-database-search-ms-dia-dftr3nm6 | Nicholas Sherman | TITLE: BAF_Protocol_013_Lipidomics: Database Search MS-DIAL and Analysis using Metaboanalyst 6.0
AUTHORS: Nicholas Sherman
[DESCRIPTION]
These steps represent a starting point for analysis of lipidomics data. Other data analysis and/or packages may be used.
[STEPS]
SECTION: MS-DIAL V 4.9.221218 starting
1. Thermo RAW... | ["[MS-DIAL V 4.9.221218 starting] Thermo RAW files are organized in two folders - one for all positive mode raw files and another for all negative mode raw files. These files will be loaded into MS-DIAL in two separate searches. Download MSP library files for Positive and Negative mode and save them in a folder in the ... |
null | null | null | dx.doi.org/10.17504/protocols.io.ehgbb3w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Charcoal treatment of seawater for removal of organics. This is an updated approach to that used by Carlucci & Silbernagiel, 1970. The approach was used in Paerl et al., 2015 Limnology and Oceanography; DOI: 10.1002/lno.10009
[BEFORE_START]
Obtain: <br />2 designated flas... | [] |
106,608 | Automated immunodetection of proteinase-K-resistant Lewy Pathology in FFPE human colon tissue | 0 | dx.doi.org/10.17504/protocols.io.kxygxy8ekl8j/v1 | https://www.protocols.io/view/automated-immunodetection-of-proteinase-k-resistan-dkcq4svw | Elizabeth Videlock | TITLE: Automated immunodetection of proteinase-K-resistant Lewy Pathology in FFPE human colon tissue
AUTHORS: Elizabeth Videlock
[DESCRIPTION]
Automated detection of gut Lewy Pathology using Leica Bond RX processor based on Protocol F using Bond Polymer Refine Detection kit (Leica Biosystems, Cat#: DS9800). Adapted fr... | ["Leica Bond RX routine factory based “Bake and Dewax” protocol.", "Enzyme Antigen Retrieval: Incubate in Enzyme 1 solution for 20 min at Room temperature", "Incubate in Peroxide Block for 5 min", "Incubate in Bond Wash buffer three times each.", "Primary antibody: Incubate in 1:10,000\n for 60 min at Room tempera... |
58,053 | Multisite Gateway Calculations: Excel spreadsheet | 3 | dx.doi.org/10.17504/protocols.io.b4xdqxi6 | https://www.protocols.io/view/multisite-gateway-calculations-excel-spreadsheet-b4xdqxi6 | Christian Mosimann | TITLE: Multisite Gateway Calculations: Excel spreadsheet
AUTHORS: Christian Mosimann
[DESCRIPTION]
Multisite Gateway cloning provides a modular tool to assemble plasmid vectors suitable for numerous applications. The assembly of Multisite Gateway plasmids requires the correct ratios of three entry vectors and the des... | [] |
29,749 | Sample preparation and imaging for large scale 3D spectral confocal imaging of tissues | null | dx.doi.org/10.17504/protocols.io.9avh2e6 | null | Tarek Ashkar, Michael Ferkowicz | TITLE: Sample preparation and imaging for large scale 3D spectral confocal imaging of tissues
AUTHORS: Tarek Ashkar, Michael Ferkowicz
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Frozen participant samples are first sized and preserved for high-resolution 3D imaging. Preserved biopsy sections ar... | ["[Label Free Imaging of Tissue]\nMount sample on uncharged slide in PBS using prepositioned piece of spacer tape to support coverslip.", "[Label Free Imaging of Tissue]\nGently push coverslip to the sample and seal with rubber cement dispensed from a syringe and 16 G needle.", "[Label Free Imaging of Tissue]\nConfigur... |
93,524 | qPCR standard for library quantification | 3 | dx.doi.org/10.17504/protocols.io.bp2l6xwd5lqe/v1 | https://www.protocols.io/view/qpcr-standard-for-library-quantification-c7juzknw | Ayinuer Aximu Petri, Matthias Meyer | TITLE: qPCR standard for library quantification
AUTHORS: Ayinuer Aximu Petri, Matthias Meyer
[DESCRIPTION]
Protocol for the preparation of a standard for DNA library quantification by quantitative real-time PCR (Meyer et al. 2008; Gansauge et al. 2020).
References
Meyer, M., Briggs, A. W., Maricic, T., Höber, B., H... | [] |
27,704 | IHC Fluorescent Frozen Sections | null | dx.doi.org/10.17504/protocols.io.7ayhifw | null | Elizabeth Smith | TITLE: IHC Fluorescent Frozen Sections
AUTHORS: Elizabeth Smith
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Immunohistochemistry protocol used for staining with fluorescent secondary antibodies to highlight specific tissue structures.</div></div>
[STEPS]
?. Day 1: Using the PAP Pen, carefully d... | ["Day 1: Using the PAP Pen, carefully draw a water barrier circle around the tissue sections on the slide – allow this circle to dry for several seconds or up to approx. one minute", "Rinse slides with PBS (pH 7.3-7.4): 4 x 5 min each", "Rinse slides with 0.5% BSA + 0.4% Triton X-100 in PBS): 1 x 10 min", "Remove slid... |
25,309 | Hornwort sporophyte induction - Bonn | null | dx.doi.org/10.17504/protocols.io.4x5gxq6 | null | Eftychis Frangedakis | TITLE: Hornwort sporophyte induction - Bonn
AUTHORS: Eftychis Frangedakis
[STEPS]
?. Grow small thallus fragments for two weeks in petri dishes on Knop or BCD media at pH 5.7 and containing 0.7% (w/v) Gelzan (A in figure).
?. Transfer plants to Magenta pots on Knop medium or BCD medium (B and C in figure)at pH 5.7, co... | ["Grow small thallus fragments for two weeks in petri dishes on Knop or BCD media at pH 5.7 and containing 0.7% (w/v) Gelzan (A in figure).", "Transfer plants to Magenta pots on Knop medium or BCD medium (B and C in figure)at pH 5.7, containing 0.7% (w/v) Gelzan. Add 2mL of sterile water into the pot using a pipette.",... |
28,422 | Sequencing and sequence analysis | null | dx.doi.org/10.17504/protocols.io.7zehp3e | null | Norfitriah Mohamed Sohaimi, Mohd Hair Bejo, Abdul Rahman Omar, Aini Ideris, Nurulfiza Mat Isa | TITLE: Sequencing and sequence analysis
AUTHORS: Norfitriah Mohamed Sohaimi, Mohd Hair Bejo, Abdul Rahman Omar, Aini Ideris, Nurulfiza Mat Isa
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.egubbww | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol provides a method for assigning whole metagenome taxonomy using MetaPhlAn and MEGAN toolkits. Based on the methods from the following publication:<br /><br />Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Divers... | [] |
87,622 | Sanger Tree of Life Sample Preparation: Triage and Dissection | 4 | dx.doi.org/10.17504/protocols.io.x54v9prmqg3e/v1 | https://www.protocols.io/view/sanger-tree-of-life-sample-preparation-triage-and-cztex6je | Jessie Jay, Halyna Yatsenko, Juan Pablo Narváez-Gómez, Haddijatou Mbye, Maria Morra, Michelle Strickland, Clare Cornwell, Caroline Howard | TITLE: Sanger Tree of Life Sample Preparation: Triage and Dissection
AUTHORS: Jessie Jay, Halyna Yatsenko, Juan Pablo Narváez-Gómez, Haddijatou Mbye, Maria Morra, Michelle Strickland, Clare Cornwell, Caroline Howard
[DESCRIPTION]
This protocol describes the process of sample preparation for the extraction of DNA and/o... | ["[Laboratory procedure] Select the first sample from the cold rack.", "[Laboratory procedure] Inspect the sample and check the sample preservation method. \nFor snap frozen samples (‘standard samples’), proceed to step 3. \nFor samples that have not been snap frozen (‘non-standard samples’), please refer to step 7.", ... |
null | null | null | dx.doi.org/10.17504/protocols.io.izfcf3n | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.itbcein | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>March, 2012, based on RNAqueous May 29, 2008 protocol revision C, phenol/chloroform protocol (http://cshprotocols.cshlp.org/content/2010/6/pdb.prot5438.full), ethanol precipitation protocol (http://cshprotocols.cshlp.org/content/2010/6/pdb.prot5440.full)</p>
[GUIDELINES]
<p>... | [] |
35,174 | Building a molecule for docking using PubChem and YASARA | 1 | null | https://www.protocols.io/view/building-a-molecule-for-docking-using-pubchem-and-bekejcte | Chris Berndsen | TITLE: Building a molecule for docking using PubChem and YASARA
AUTHORS: Chris Berndsen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The database </span><a href="https://pubchem.ncbi.nlm.nih.gov/" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">Pubchem</spa... | ["[Find the structure of your molecule]\nNavigate to PubChem", "[Find the structure of your molecule]\nSearch for the molecule name\nThe molecule will like not be a protein, but a small molecule like glucose or maltodextrin. Searching for proteins is not useful.", "[Find the structure of your molecule]\nSelect an entry... |
102,860 | TDP-43 RNA aptamer staining to detect pathological TDP-43 in FFPE human tissue, as described in Spence and Waldron et al., 2024 (Acta Neuropathologica): A SOP and tick-sheet. v2. | 0 | dx.doi.org/10.17504/protocols.io.eq2lyjo4mlx9/v2 | https://www.protocols.io/view/tdp-43-rna-aptamer-staining-to-detect-pathological-dgpk3vkw | Fergal M Waldron, Holly Spence, Jenna Gregory | TITLE: TDP-43 RNA aptamer staining to detect pathological TDP-43 in FFPE human tissue, as described in Spence and Waldron et al., 2024 (Acta Neuropathologica): A SOP and tick-sheet. v2.
AUTHORS: Fergal M Waldron, Holly Spence, Jenna Gregory
[DESCRIPTION]
Here we provide a SOP to outline the correct procedures for perf... | [] |
102,277 | Synapse Quantification with Puncta Analyzer | 0 | dx.doi.org/10.17504/protocols.io.8epv5rnddg1b/v1 | https://www.protocols.io/view/synapse-quantification-with-puncta-analyzer-df5d3q26 | Justin T Savage | TITLE: Synapse Quantification with Puncta Analyzer
AUTHORS: Justin T Savage
[DESCRIPTION]
Protocol for installing Puncta Analyzer and using it to analyze synapse images.
[STEPS]
SECTION: Install Puncta Analyzer
1. Go to https://github.com/toddstavish/puncta-analyzer and follow the following instructions for instal... | ["[Install Puncta Analyzer] Go to https://github.com/toddstavish/puncta-analyzer and follow the following instructions for installing Puncta Analyzer", "[Install Puncta Analyzer] Install JDK", "[Install Puncta Analyzer] Install maven", "[Install Puncta Analyzer] Fork the puncta analyzer repo", "[Install Puncta Analyzer... |
49,717 | Analysis of glycosphingolipids from human plasma | 1 | dx.doi.org/10.17504/protocols.io.busvnwe6 | https://www.protocols.io/view/analysis-of-glycosphingolipids-from-human-plasma-busvnwe6 | David A Priestman, Danielle te Vruchte, Kerri-Lee Wallom, María E Fernández-Suárez, Maria Leondaraki, Carissa Drake , Frances M Platt | TITLE: Analysis of glycosphingolipids from human plasma
AUTHORS: David A Priestman, Danielle te Vruchte, Kerri-Lee Wallom, María E Fernández-Suárez, Maria Leondaraki, Carissa Drake , Frances M Platt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Interest in the role of cellular glycosphingolipids (... | ["[GSL preparation from plasma]\nUse plasma for GSL quantification.\n50 µl", "[GSL preparation from plasma]\nAdd de-ionised water to make the volume up to in a 1.5 ml screw-cap tube.\n150 µl\n200 µl", "[GSL preparation from plasma]\nAdd of chloroform/methanol (1:2, v/v) to give (C/M/W 4:8:3 final).\n0.8 mL\nChlorof... |
95,717 | Study Protocol: Using Video Testimonials as a School-Based Social Marketing Intervention in Adolescent Smoking Cessation Programs | 0 | dx.doi.org/10.17504/protocols.io.3byl4qpbjvo5/v1 | https://www.protocols.io/view/study-protocol-using-video-testimonials-as-a-schoo-c9qdz5s6 | Mohammad Eko Fitrianto, Basu Swastha Dharmmesta, Bernardinus Maria Purwanto | TITLE: Study Protocol: Using Video Testimonials as a School-Based Social Marketing Intervention in Adolescent Smoking Cessation Programs
AUTHORS: Mohammad Eko Fitrianto, Basu Swastha Dharmmesta, Bernardinus Maria Purwanto
[DESCRIPTION]
This protocol study intended to describe the examination process of emotion-based s... | ["[Phase I: Preparation] Study design", "[Phase I: Preparation] Decide to use control condition (variables and group control)\nTo minimize the possibility of alternative answer, researcher must control the participants age and sex. Control condition may imply with this situation: participants and the actors are in the ... |
48,382 | Patient satisfaction survey at the multidisciplinary knee osteoarthritis clinic | 1 | dx.doi.org/10.17504/protocols.io.btg6njze | https://www.protocols.io/view/patient-satisfaction-survey-at-the-multidisciplina-btg6njze | Rebeca Dias, Fabricio Loures, Henrique Chamarelli, João Henrique Reis | TITLE: Patient satisfaction survey at the multidisciplinary knee osteoarthritis clinic
AUTHORS: Rebeca Dias, Fabricio Loures, Henrique Chamarelli, João Henrique Reis
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">To evaluate the satisfaction of patients undergoing conservative multidisciplinary tre... | ["To survey de medical records of all patients who treated at the multidisciplinary knee osteoarthritis outpatient clinic at Hospital Universitário Pedro Ernesto.", "Make contact and explain the content and importance of the research to improve the quality of assistance.", "Conduct the interview with eight questions fo... |
108,966 | Systemic AAV administration through retro-orbital injection | 0 | dx.doi.org/10.17504/protocols.io.36wgqnw73gk5/v1 | https://www.protocols.io/view/systemic-aav-administration-through-retro-orbital-dnne5dbe | Gerard Michael Coughlin | TITLE: Systemic AAV administration through retro-orbital injection
AUTHORS: Gerard Michael Coughlin
[DESCRIPTION]
Adeno-associated viruses (AAVs) are popular tools for gene delivery to animal models. Natural and engineered AAVs can even achieve widespread transduction of target organs through systemic delivery. Fast (... | ["[Systemic AAV administration through retro-orbital injection] Before beginning, ensure that all AAVs are prepared and recently (< 1 month) titered. If injecting multiple AAVs (either into the same animals, or animals in separate conditions), we recommend that all AAVs are titered in the same qPCR or ddPCR assay. \n\n... |
94,581 | BAF_Protocol_004 LC-MS(/MS) nLC EASY LC1200 and Orbitrap Exploris 480 | 1 | dx.doi.org/10.17504/protocols.io.81wgbxb63lpk/v1 | https://www.protocols.io/view/baf-protocol-004-lc-ms-ms-nlc-easy-lc1200-and-orbi-c8kvzuw6 | nesf | TITLE: BAF_Protocol_004 LC-MS(/MS) nLC EASY LC1200 and Orbitrap Exploris 480
AUTHORS: nesf
[DESCRIPTION]
This protocol gives a basic set up with LC (nLC 1200) and Instrument (Thermo Exploris 480) conditions for running proteomics samples with low to moderate complexity. This is what we use and can be adapted by shorte... | ["[Prepare samples for injection] Suspend digested peptides with 20-40 uL of 0.1% FA.", "[Prepare samples for injection] Vortex, microfuge for 15 minutes at max speed, and take off 10uL into autosampler vial (do not touch bottom of tube). Use gel loading tip to transfer the volume to autosampler vial to prevent carryin... |
42,391 | In vivo imaging of acetylcholine release in the peripheral nervous system with a fluorescent nanosensor | 1 | dx.doi.org/10.17504/protocols.io.bmmxk47n | https://www.protocols.io/view/in-vivo-imaging-of-acetylcholine-release-in-the-pe-bmmxk47n | Junfei Xia, Hongrong Yang, Michelle Mu, Nicholas Micovic, Kira E. Poskanzer, James Monaghan, Heather Clark | TITLE: In vivo imaging of acetylcholine release in the peripheral nervous system with a fluorescent nanosensor
AUTHORS: Junfei Xia, Hongrong Yang, Michelle Mu, Nicholas Micovic, Kira E. Poskanzer, James Monaghan, Heather Clark
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol provi... | ["[In vivo staining of submandibular ganglion with nanosensor by microinjection ]\n1. Anesthetize the mouse by inhalation of 1.5-2% and place it on a rodent heating pad set at 2. Place the mouse on its dorsal side under the dissection scope.3. Apply depilatory cream to the neck region for and remove the hair.4. Make... |
27,365 | Bangladesh National Serosurvey Vibriocidal Protocol (96-well plate, OD600, mAb) | null | dx.doi.org/10.17504/protocols.io.6ydhfs6 | null | Taufiqur Bhuiyan, Jason Harris, Owen Jensen, Daniel Leung, Andrew Azman, Firdausi Qadri | TITLE: Bangladesh National Serosurvey Vibriocidal Protocol (96-well plate, OD600, mAb)
AUTHORS: Taufiqur Bhuiyan, Jason Harris, Owen Jensen, Daniel Leung, Andrew Azman, Firdausi Qadri
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol provides the details on how vibriocidal assays w... | ["Streak V. cholerae (O1 Ogawa or Inaba) onto blood agar and incubate at overnight (approx )\n37 °C", "Inoculate a loopful of bacteria from the blood agar plate (1-2 colonies) into 15mL culture tube from previous step", "Incubate in an orbital shaker-incubator at and 220 rpm for -\n37 °C", "While the bacterial cultu... |
102,234 | Tomogram reconstruction and sub-tomogram averaging to obtain full-length, auto-inhibited LRRK2 filaments on microtubules | 0 | dx.doi.org/10.17504/protocols.io.n92ld89zxv5b/v2 | https://www.protocols.io/view/tomogram-reconstruction-and-sub-tomogram-averaging-df323qqe | Siyu Chen, Josh Hutchings, Digvijay Singh | TITLE: Tomogram reconstruction and sub-tomogram averaging to obtain full-length, auto-inhibited LRRK2 filaments on microtubules
AUTHORS: Siyu Chen, Josh Hutchings, Digvijay Singh
[DESCRIPTION]
This protocol describes all the data analysis steps after obtaining the LRRK2 on microtubule dataset.
[STEPS]
SECTION: Microt... | ["[Microtubule tracing, LRRK2 sub-tomogram picking and ab-initio model building in Dynamo] IMOD was used to manually go through all reconstructed tomograms and trace the backbone of microtubules. For each intact microtubule, make an open trajectory model by pressing the middle mouse button along the microtubule axis an... |
61,600 | SH-SY5Y culturing | 4 | dx.doi.org/10.17504/protocols.io.bp2l617jzvqe/v1 | https://www.protocols.io/view/sh-sy5y-culturing-b8d8rs9w | Laura Smith, David C | TITLE: SH-SY5Y culturing
AUTHORS: Laura Smith, David C
[DESCRIPTION]
SH-SY5Y neuroblastoma cells were cultured in a 1:1 mixture of Ham’s F-12 and DMEM growth medium supplemented with 10% FBS, non-essential amino acids (NEAA: 0.1 mM of: glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline and... | ["[Characteristics] ATCC # CRL-2266\nHuman neuroblastoma cell line, cloned from SH-SY5, which is from SH-SY, which is from SK-N-SH. The original cell line was isolated from a woman's metastatic bone tumor in 1970.\nKnown to be dopamine beta hydroxylase active, acetylcholinergic, glutamatergic ... |
82,829 | Test if posted draft can later be published with DOI | 1 | null | https://www.protocols.click/view/test-if-posted-draft-can-later-be-published-with-d-cu5mwy46 | Melinda Chue Donahey | TITLE: Test if posted draft can later be published with DOI
AUTHORS: Melinda Chue Donahey
[DESCRIPTION]
Test if posted draft can later be published with DOI
[STEPS] | [] |
13,043 | illumina MiSeq Dual Index Amplicon Sequencing Sample Preparation Bacterial 16S rRNA gene | 1 | dx.doi.org/10.17504/protocols.io.qytdxwn | https://www.protocols.io/view/illumina-miseq-dual-index-amplicon-sequencing-samp-qytdxwn | Alexander Eiler, Omneya Osman, Anna Szekely | TITLE: illumina MiSeq Dual Index Amplicon Sequencing Sample Preparation Bacterial 16S rRNA gene
AUTHORS: Alexander Eiler, Omneya Osman, Anna Szekely
[DESCRIPTION]
Preparation of PCR products for amplicon sequencing
[BEFORE_START]
Put pipettes and tips in the UV chamber for 10 mins.
Clean bench with MQ and EtOH.
[GUI... | ["Perform the first PCR (triplicates/duplicates of each sample) using Illumina adaptor attached primers that target the gene of your choice. Here we present the protocol using the Bacteria primers 341F and 805RN. For the forward primer cite: Herlemann et al., 2011 and for the reverse primer cite based on: Apprill et al... |
86,894 | Can light be used to treat obesity and diabetes? | 4 | dx.doi.org/10.17504/protocols.io.j8nlkooj1v5r/v4 | https://www.protocols.io/view/can-light-be-used-to-treat-obesity-and-diabetes-cy4nxyve | Rédoane Daoudi | TITLE: Can light be used to treat obesity and diabetes?
AUTHORS: Rédoane Daoudi
[DESCRIPTION]
This document is highly theoretical lab method paper. It may be used to perform thermogenesis from several cells in a living organism instead of only brown adipocytes. We perform thermogenesis from white adipocytes. The purpo... | ["[Introduction] This document is highly theoretical lab method paper. It may be used to perform thermogenesis from several cells in a living organism instead of only brown adipocytes. We perform thermogenesis from white adipocytes. The purpose is to uptake the blood glucose and lipids to produce heat and subsequent we... |
47,590 | Immunofluorescence on FFPE tissue sections from inflamed and non-inflamed human gut | 1 | dx.doi.org/10.17504/protocols.io.bsqendte | https://www.protocols.io/view/immunofluorescence-on-ffpe-tissue-sections-from-in-bsqendte | Ross J Porter, Katie J Smith, Gwo-Tzer Ho, Emily Gwyer Findlay | TITLE: Immunofluorescence on FFPE tissue sections from inflamed and non-inflamed human gut
AUTHORS: Ross J Porter, Katie J Smith, Gwo-Tzer Ho, Emily Gwyer Findlay
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">Analysis of immunofluorescence is commo... | ["[Deparaffinisation & Tissue Rehydration]\nSequentially immerse the slides, in the slide rack, into the following solutions:Xylene Xylene 100% alcohol 100% alcohol 90% alcohol 80% alcohol 70% alcohol See image in Step 6 for the benefits of these enhanced timings.", "[Deparaffinisation & Tissue Rehydration]\nPlace the... |
null | null | null | dx.doi.org/10.17504/protocols.io.tsvene6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Background
Physical activity reduces the risk of noncommunicable diseases and is therefore an
essential component of a healthy lifestyle. Regular engagement in physical activity can
produce immediate and long term health benefits. However, physical activity levels are
not as hig... | ["[Define the review question] To identify the statistical methods used to evaluate the physical activity programs\nTo identify the statistical methods and models used to model the physical activity", "[Search] The search will be carried on online data bases (namely Pubmed/Medline, Web of Science and CINAHL) And will b... |
null | null | null | dx.doi.org/10.17504/protocols.io.hfzb3p6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
68,920 | Adult mouse lung cell dissociation (on ice) | 1 | dx.doi.org/10.17504/protocols.io.4r3l2ddjg1y9/v4 | https://www.protocols.io/view/adult-mouse-lung-cell-dissociation-on-ice-cfiytkfw | Andrew Potter | TITLE: Adult mouse lung cell dissociation (on ice)
AUTHORS: Andrew Potter
[DESCRIPTION]
This protocol was used to dissociate adult (8-10 wk) mouse lung tissue. The entire procedure is carried out on ice (to reduce artifact gene expression changes) and takes about half an hour. The yield was 16,240 non-RBC/mg tissue wi... | ["[Isolate tissue] Isolate lung (optional: perfuse lung with DPBS to reduce RBC). Place lung in ice-cold DPBS and transport on ice.", "[Isolate tissue] Using sterile forceps, transfer lung tissue to petri dish on ice. Remove excess DPBS using pipet. Mince lung tissue on petri dish on ice for 2 min until fine paste. Vig... |
78,626 | ONT DNA Barcoding Fungal Amplicons w/ MinION & Flongle | 2 | null | https://www.protocols.io/view/ont-dna-barcoding-fungal-amplicons-w-minion-amp-fl-cq2avyae | Stephen Douglas Russell | TITLE: ONT DNA Barcoding Fungal Amplicons w/ MinION & Flongle
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
This collection of protocols outline a working methodology to DNA barcode fungal specimens. This process will work for dried tissue, fresh tissue, or with DNA template that has already gone through an... | [] |
57,951 | Primary cortical neuronal culture | 4 | dx.doi.org/10.17504/protocols.io.b4t7qwrn | https://www.protocols.io/view/primary-cortical-neuronal-culture-b4t7qwrn | Xiqun Chen, Qing Ye | TITLE: Primary cortical neuronal culture
AUTHORS: Xiqun Chen, Qing Ye
[DESCRIPTION]
Primary cortical neurons were prepared from C57BL/6J mice embryonic day 17. The dissected cortical tissue was digested, triturated, and centrifuged. Cells were plated onto poly (L-lysine)-coated 24-well plates at 106 cells per well and... | ["For Primary cortical neuronal culture - Use C57BL/6J mice at embryonic day 17", "Anesthetized pregnant mice (1% sodium pentobarbital, 80mg/kg), dissect their embryos and collect the cortex. \n(Separate and remove the soft membrane and blood vessels, rinse the cerebral cortex in PBS, and use the ophthalmic scissor to... |
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