id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.vn4e5gw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | ["fds"] |
null | null | null | dx.doi.org/10.17504/protocols.io.vhce32w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Objective: To feed individual house flies a specific amount of bacteria in order to
determine bacteria “fate” (persistence, via enumeration; spatiotemporal location, via
microscopy) and house fly immune response (whole fly or tissue-specific, via downstream mRNA or protein expre... | ["[Preparing House Flies for Bacterial Feeding] {\"blocks\":[{\"key\":\"3g659\",\"text\":\"Collect pupae from colony and surface sanitize for 2 minutes each with gentle swirling in 10% bleach, sterile H2O, 100% ethanol, sterile\\u00a0H2O. \",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[{\"offset\":111,\"len... |
null | null | null | dx.doi.org/10.17504/protocols.io.e89bhz6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="view-protocol-title vpt-block">
<p>This protocol is part of the FOCUS™ Mitochondria Kit <a href="https://www.protocols.io/view/Collection-of-FOCUS-Mitochondria-Kit-protocols-e8tbhwn" target="_blank">collection</a>. Please refer to the appropriate protocol depending o... | [] |
26,154 | Prepare NGM plates with fungzizone | null | dx.doi.org/10.17504/protocols.io.5sig6ce | null | Research Cancer UK / Wellcome Gurdon Institute media kitchen | TITLE: Prepare NGM plates with fungzizone
AUTHORS: Research Cancer UK / Wellcome Gurdon Institute media kitchen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Prepare NGM plates with fungizon</div></div>
[STEPS]
?.
?. ABCD1Ingredients Quantity2NGM Media 1L3Cholesterol 5mg/ml1ml41M CaCl21ml51M M... | ["ABCD1Ingredients Quantity2NGM Media 1L3Cholesterol 5mg/ml1ml41M CaCl21ml51M MgSO41ml61M KH2PO425ml7Petri dish30mmas required8 50mmas required9 90mmas required10Fungizone400 microlitres 1 microtubbe\nABCD1Ingredients Quantity2NGM Media 1L3Cholesterol 5mg/ml1ml41M CaCl21ml51M MgSO41ml61M KH2PO425ml7Petri dish30mmas... |
75,988 | Expansion of NK cells on feeder cells | 1 | dx.doi.org/10.17504/protocols.io.5qpvorqebv4o/v1 | https://www.protocols.click/view/expansion-of-nk-cells-on-feeder-cells-cnfuvbnw | Philippa R Kennedy | TITLE: Expansion of NK cells on feeder cells
AUTHORS: Philippa R Kennedy
[DESCRIPTION]
This protocol contains the details of how we implement the protocol devised by Somanchi et al. (https://www.jove.com/v/2540/expansion-purification-functional-assessment-human-peripheral-blood).
5 million enriched NK cells should gi... | ["[Enrich NK cells] Start with 5x106 NK cells enriched from PBMC (EasySep Human NK Cell Enrichment Kit, Cat. 19055, STEMCELL Technologies)", "[Enrich NK cells] For each 5x106 NK, count and irradiate 1x107 K562 mIL-21 41BBL using a gamma irradiator at 100 Gy", "[Enrich NK cells] Post irradiation, wash the cells with PBS... |
44,079 | TaqMan SNP genotyping protocol | 4 | dx.doi.org/10.17504/protocols.io.bpapmidn | https://www.protocols.io/view/taqman-snp-genotyping-protocol-bpapmidn | Heather Robeson, Jing Jin, Mohammed S. Orloff | TITLE: TaqMan SNP genotyping protocol
AUTHORS: Heather Robeson, Jing Jin, Mohammed S. Orloff
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The protocol is based on TaqMan SNP genotyping protocol</div></div>
[STEPS]
?. [PCR reactions]
384-well Fast (5‐μL reaction ) Prepare the reaction mix:Combine... | ["[PCR reactions]\n384-well Fast (5‐μL reaction ) Prepare the reaction mix:Combine the following components for the number of reactions required, plus 10% overage. 2X TaqMan Master Mix: 20X Assay Working Stock: Total volume per well:\n2.5 µl\n0.25 µl\n2.75 µl", "[PCR reactions]\nVortex to mix", "[PCR reactions]\nCent... |
52,234 | Chickpea Inoculation with A. rabiei for Ascochyta Blight Disease Assessment Under Controlled Conditions | 4 | dx.doi.org/10.17504/protocols.io.bw9iph4e | https://www.protocols.io/view/chickpea-inoculation-with-a-rabiei-for-ascochyta-b-bw9iph4e | Melody Christie, Dr Prabhakaran Sambasivam, Ido Bar | TITLE: Chickpea Inoculation with A. rabiei for Ascochyta Blight Disease Assessment Under Controlled Conditions
AUTHORS: Melody Christie, Dr Prabhakaran Sambasivam, Ido Bar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the inoculation preparation, bioassay and disea... | ["[Inoculum preparation]\nApproximately 2 weeks before the planned inoculation date, subculture isolates of A. rabiei onto V8 media agar which contains 30mg/L of antibiotic (Streptomycin/Penicillin ). Add antibiotic after media has been autoclaved and is cool enough to touch (approximately ). Aseptically place one p... |
56,817 | Ion exchange chromatography for small extracellular vesicles isolation | 4 | dx.doi.org/10.17504/protocols.io.3byl4b71zvo5/v1 | https://www.protocols.io/view/ion-exchange-chromatography-for-small-extracellula-b3qrqmv6 | Ricardo Malvicini, Diego Santa Cruz, Anna Maria Tolomeo, Maurizio Muraca, Gustavo Yannarelli, Natalia Pacienza | TITLE: Ion exchange chromatography for small extracellular vesicles isolation
AUTHORS: Ricardo Malvicini, Diego Santa Cruz, Anna Maria Tolomeo, Maurizio Muraca, Gustavo Yannarelli, Natalia Pacienza
[DESCRIPTION]
In the last few years, extracellular vesicles have become of great interest due to its potential as biomark... | ["[Ion Exchange Chromatography] Mix well and add 5 mL of anion exchange resin (Q Sepharose Fast Flow, GE Health Care Life Science, CAT# 17051001) to an empty column (Bio-Rad, 7321010) at RT and wait for the resin to sediment; remove the end cap and let the ethanol flow out. Connect the end cap, 4 mL of resin bed volume... |
99,981 | Fragment Analyzer Operation for PCR Products | 1 | dx.doi.org/10.17504/protocols.io.5jyl8mxwrg2w/v2 | https://www.protocols.io/view/fragment-analyzer-operation-for-pcr-products-ddvm2646 | Allen Institute for Brain Science | TITLE: Fragment Analyzer Operation for PCR Products
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
This protocol describes fragment analyzer operation for polymerase chain reaction (PCR) products. The Fragment Analyzer by Advanced Analytical is used as a quality control step for genotyping PCR products. Pea... | [] |
22,819 | Human IHC staining conditions | 3 | dx.doi.org/10.17504/protocols.io.2ibgcan | https://www.protocols.io/view/human-ihc-staining-conditions-2ibgcan | Kristin Anderson | TITLE: Human IHC staining conditions
AUTHORS: Kristin Anderson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Staining conditions for human IHC.</div></div>
[STEPS] | [] |
85,494 | NucBarcoder - a bioinformatic pipeline to characterise the genetic basis of plant species differences | 5 | null | https://www.protocols.io/view/nucbarcoder-a-bioinformatic-pipeline-to-characteri-cxqwxmxe | Wu Huang, Alex Twyford, Peter Hollingsworth | TITLE: NucBarcoder - a bioinformatic pipeline to characterise the genetic basis of plant species differences
AUTHORS: Wu Huang, Alex Twyford, Peter Hollingsworth
[DESCRIPTION]
There is now a significant number of studies that have sequenced multiple loci from the nuclear genomes of plants and these are available in pu... | ["[Data cleaning and filtering] Data cleaning and filtering were performed at both the individual and locus levels. \nDifferent clean-up and filtering tools were used for aligned sequence formats and SNP matrices. VCFtools (0.1.17) (Danecek et al., 2011) was used to deal with the vcf format and self-written scripts wer... |
81,818 | NCEM Drop conventional (TM-014) | 4 | dx.doi.org/10.17504/protocols.io.36wgqjx95vk5/v1 | https://www.protocols.io/view/ncem-drop-conventional-tm-014-ct52wq8e | sandra.crameri | TITLE: NCEM Drop conventional (TM-014)
AUTHORS: sandra.crameri
[DESCRIPTION]
Standard routine conventional negative contrast EM.
[STEPS]
SECTION: HEADER
1. SAN:
SPEC No:
OPERATOR:
SECTION: Conventional
2. Adsorb 10 µL sample to grid 10 min , visually inspect to ensure sample does not dry out in this time.
... | ["[HEADER] SAN:\n\n\n\n\nSPEC No:\n\n\n\n\nOPERATOR:", "[Conventional] Adsorb 10 µL sample to grid 10 min , visually inspect to ensure sample does not dry out in this time.", "[Conventional] Drain excess sample from grid using filter paper, leave wet.", "[Conventional] Stain 1 min", "Drain & dry using filter paper"] |
87,681 | Détection directe du poliovirus par séquençage nanopore V2 | 1 | dx.doi.org/10.17504/protocols.io.81wgbxrynlpk/v1 | https://www.protocols.io/view/d-tection-directe-du-poliovirus-par-s-quen-age-nan-czu9x6z6 | Alex Shaw, Manasi Majumdar, Catherine Troman, Erika Bujaki, Joyce Akello, Aine.OToole, shannon.fitz, c.ansley, rachel.colquhoun, arshady, khurshida, alammu, Andrew Rambaut, Javier Martin, Nick Grassly | TITLE: Détection directe du poliovirus par séquençage nanopore V2
AUTHORS: Alex Shaw, Manasi Majumdar, Catherine Troman, Erika Bujaki, Joyce Akello, Aine.OToole, shannon.fitz, c.ansley, rachel.colquhoun, arshady, khurshida, alammu, Andrew Rambaut, Javier Martin, Nick Grassly
[DESCRIPTION]
ABSTRAIT
Ce protocole est... | ["[Préparation d'ADN - Protocole pour l'amplification VP1nichée] Synthèse de cADN à l'aide du mélange réactionnel Superscript III One pot et des amorces PanEV", "[Préparation d'ADN - Protocole pour l'amplification VP1nichée] Amplification VP1 à code-barres avec DreamTaq", "[Préparation d'ADN - ... |
19,367 | Enterovirus (EV) A71 TaqMan 2018 (EV-A71-TM2018) | null | dx.doi.org/10.17504/protocols.io.w6ffhbn | null | Ian Mackay, Judy Northill | TITLE: Enterovirus (EV) A71 TaqMan 2018 (EV-A71-TM2018)
AUTHORS: Ian Mackay, Judy Northill
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol aims to amplify enterovirus (EV) A71 viruses but not other viruses.</div><div class = "text-block">This protocol was designed by us.</div><div clas... | ["[Oligonucleotide sequences]\nAB1NameSequence 5'-3'2EVA71-VP4-For1TAYTAYAAAGAYTCBTATGCYG3EVA71-VP4-Rev1CCTTRACAGGRTTWGCRAACTT4EVA71-VP4-Rev2CTTTRACAGGRTTWGCAAATTT5EVA71-VP4-Rev3CCTTCACAGGGTTCGCAAACTT6EVA71-VP4-P1FAM - ACAGCVGGCAAGCAGAGYCTCAA - BHQ17EVA71-VP4-P2FAM - ACAGCRGGYAAACAGAGYCTCAA - BHQ18EVA71-VP4-P3FAM - ACT... |
null | null | null | dx.doi.org/10.17504/protocols.io.gzibx4e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Samples for LC-MS analysis.</p>
[STEPS]
?.
?.
?. | [] |
63,264 | withdrawal protocol for study of heavy metals in hair with edx | 1 | dx.doi.org/10.17504/protocols.io.5qpvob8zxl4o/v1 | https://www.protocols.io/view/withdrawal-protocol-for-study-of-heavy-metals-in-h-b9z8r79w | Salvatore Del Prete, Daniela Marasco, Antonella Cicale | TITLE: withdrawal protocol for study of heavy metals in hair with edx
AUTHORS: Salvatore Del Prete, Daniela Marasco, Antonella Cicale
[DESCRIPTION]
Whenever possible, it is preferable to take the sample from the area corresponding to the back of the head (vertex), as close as possible to the scalp;
in fact, it is ... | ["Roll and string a pencil-thick strand of hair or several thinner strands from the back of the head.", "Hair should be cut immediately above the skin, as close to the scalp as possible. It is necessary to note the length of the hair strand.", "The lock of hair must be placed in the appropriate aluminium foil. The port... |
null | null | null | dx.doi.org/10.17504/protocols.io.c5by2m | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol gives a method for treatment and preservation of seawater samples that will be subject to fluorescence in situ hybridization (FISH). Samples are used with fluorescent probes to determine what populations of bacteria or other microbes are present. The filters m... | [] |
49,420 | How to make a cup of tea | 1 | null | https://www.protocols.io/view/how-to-make-a-cup-of-tea-buhknt4w | ines.boehm | TITLE: How to make a cup of tea
AUTHORS: ines.boehm
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is part of the </span><a href="https://carpentries-incubator.github.io/fair-bio-practice/06-record-keeping/index.html" style = "text-decoration:underline;color:blue;cursor:pointer... | ["[Preparation of water and tea]\nFill the kettle with ~250mL of water, or until your min fill line and boil the water.", "[Preparation of water and tea]\nIn the meantime prepare your tea bag, unpack it and place it in the cup/mug so that the tag is positioned outside of the mug.", "[Preparation of water and tea]\nOnce... |
48,197 | Chimeric Protein-LAG and Protein-LG sandwich ELISA | 1 | dx.doi.org/10.17504/protocols.io.btbdnii6 | https://www.protocols.io/view/chimeric-protein-lag-and-protein-lg-sandwich-elisa-btbdnii6 | Angel Justiz-Vaillant | TITLE: Chimeric Protein-LAG and Protein-LG sandwich ELISA
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This ELISA was used to study the interactions between protein-LAG (PLAG) and protein-LG (SpLG) with different immunoglobulin preparations from mammalian species. ... | ["This ELISA was used to study the interactions between protein-LAG (PLAG) and protein-LG (SpLG) with different immunoglobulin preparations from mammalian species. The 96 well microtiter plate was coated overnight at 4°C with 2 µg/µl per well of PLAG in carbonate-bicarbonate buffer pH 9.6.", "The plate was then treated... |
89,956 | T-4 TICK TESTING | 4 | dx.doi.org/10.17504/protocols.io.kxygx3ozzg8j/v1 | https://www.protocols.io/view/t-4-tick-testing-c34cyqsw | REDI-NET Consortium | TITLE: T-4 TICK TESTING
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol details standard operating procedure for tick testing.
[BEFORE_START]
BEFORE START
Check the DNA and RNA concentrations in each sample of total nucleic acid (TNA) extraction.
If the concentrations are detectable, choose the sequencing ... | ["[gDNA PREPARATION] When the RNA concentration of the sample is lower than the detectable range of the Qubit High Sensitivity Assay (<0.01 ng/ul), the sample is subjected to gDNA sequencing. The cDNA synthesis can be skipped.", "[gDNA PREPARATION] When the DNA concentration >10 ng/ul, calculate the required volume of ... |
65,305 | A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP and recombinant AAV6 vectors | 1 | dx.doi.org/10.17504/protocols.io.cbzzsp76 | https://www.protocols.io/view/a-precise-gene-delivery-approach-for-human-induced-cbzzsp76 | Koollawat Chupradit, nontaphat.tho , Chatchai Tayapiwatana, methichit.wat | TITLE: A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP and recombinant AAV6 vectors
AUTHORS: Koollawat Chupradit, nontaphat.tho , Chatchai Tayapiwatana, methichit.wat
[DESCRIPTION]
Genome editing in human induced pluripotent stem cells (hiPSCs) offers a potential tool ... | ["[Production of the AAV6 particles harboring GOI-mCherry] The AAV6 production is carried out by co-transfecting the pAAV donor plasmid and the pDGM6 helper plasmid into HEK293T cells. The donor plasmid contains inverted terminal repeat (ITR), left homology arm of the AAVS1 gene (AAVS1-LHA), EF1α promoter, GOI-mCherry,... |
39,321 | BGISEQ-500 （DNBSEQ-G50） Sequencing | 1 | dx.doi.org/10.17504/protocols.io.bimzkc76 | https://www.protocols.io/view/bgiseq-500-dnbseq-g50-sequencing-bimzkc76 | Jie Huang, Xinming Liang, Yuankai Xuan, Chunyu Geng, Yuxiang Li, Haorong Lu, Shoufang Qu, Xianglin Mei, Hongbo Chen, Ting Yu, Nan Sun, Junhua Rao, Jiahao Wang, Wenwei Zhang, Ying Chen, Sha Liao, Hui Jiang, Xin Liu, Zhaopeng Yang, Feng Mu, Shangxian Gao | TITLE: BGISEQ-500 （DNBSEQ-G50） Sequencing
AUTHORS: Jie Huang, Xinming Liang, Yuankai Xuan, Chunyu Geng, Yuxiang Li, Haorong Lu, Shoufang Qu, Xianglin Mei, Hongbo Chen, Ting Yu, Nan Sun, Junhua Rao, Jiahao Wang, Wenwei Zhang, Ying Chen, Sha Liao, Hui Jiang, Xin Liu, Zhaopeng Yang, Feng Mu, Shangxian Gao
[DESCRIPTION]
<... | ["Test the quality of the sequencing library (see protocol for library preparation) by the Qubit® ssDNA Assay Kit and homogenized at 6ng total amounts.", "Carry out Rolling circle amplification（RCA）for 10 minutes in an 80 ul reaction volume with pure water, buffer and DNB polymerase", "Add 20 ul DNBs stopping buffer to... |
null | null | null | dx.doi.org/10.17504/protocols.io.fdibi4e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The <strong>GUS reporter system</strong> (<em>GUS</em>: <a href="https://en.wikipedia.org/wiki/%CE%92-glucuronidase" target="_blank">β-glucuronidase</a>) is a <a href="https://en.wikipedia.org/wiki/Reporter_gene" target="_blank">reporter gene</a> system, particularly useful i... | [] |
81,347 | HCR - Embryo/Larvae fixation and permeabilisation | 4 | null | https://www.protocols.io/view/hcr-embryo-larvae-fixation-and-permeabilisation-ctpbwmin | FishFloorUCL | TITLE: HCR - Embryo/Larvae fixation and permeabilisation
AUTHORS: FishFloorUCL
[DESCRIPTION]
In situ hybridization chain reaction (HCR) is a method to visualize specific mRNAs in diverse organisms by applying a HCR that is an isothermal enzyme-free nucleotide polymerization method using hairpin DNAs. This specific pro... | ["[Day 1] Wash embryos/larvae 3 x 5 min with 1 mL PBS to stop the fixation\nNote: Ensure PFA is disposed of safely", "[Day 1] (Optional) Dissect the brains in PBS\nNote: If left undissected, skip to step 8", "[Day 1] (Optional - The HCR probes are very small, so HCR will work with no permeabilisation at all, even in un... |
53,879 | Preparation of electrocompetent cells | 4 | dx.doi.org/10.17504/protocols.io.byuxpwxn | https://www.protocols.io/view/preparation-of-electrocompetent-cells-byuxpwxn | Shuning Guo | TITLE: Preparation of electrocompetent cells
AUTHORS: Shuning Guo
[DESCRIPTION]
This protocol is used to prepare electrocompetent cells with high transformation efficiency.
[BEFORE_START]
All the medium and containers used in the protocol should be sterilized by high temperature autoclave.
All the steps that expos... | ["Line the E. coli strain on a solid LB medium without resistance and culture at 37℃ for 12h.", "Pick monoclonal cells from the medium and culture them in 5 ml LB medium at 37℃ for 12h.", "Inoculate 100 ml of LB medium with 1% volume of E. coli culture from step 2.", "Grow the cells at 37℃ shaking at 200 rpm to an OD60... |
null | null | null | dx.doi.org/10.17504/protocols.io.q92dz8e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The Odyssey Fc Imager, with 600 channel capabilities, can image agarose gels stained with popular DNA stains, such as ethidium bromide and SYBR Safe DNA stain, with sub-nanogram sensitivity. The Odyssey Fc Imager contains a 532 nm diffuse source with an excitation maximum of ... | ["[Gel Preparation] Prepare desired agarose (0.8%, 1.0%, 1.2%, etc.) in 1X TAE or 1X TBE buffer.", "[Gel Preparation] Heat to dissolve agarose.", "[Gel Preparation] Cool solution until warm to the touch (60°F) prior to pouring in casting tray.", "[Gel Preparation] Pour molten agarose solution into casting tray and set ... |
20,150 | U Mass - Energy balance – food intake, energy expenditure, physical activity | null | dx.doi.org/10.17504/protocols.io.xwwfpfe | null | Jason Kim | TITLE: U Mass - Energy balance – food intake, energy expenditure, physical activity
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block"><span>The TSE PhenoMaster/LabMaster Metabolic Cage system is used to... | ["Metabolic cage food and water baskets are calibrated.", "Drink and food baskets are filled with water and appropriate diet.", "Corncob bedding is added to the cages.", "Mice are individually housed in metabolic cages and checked daily for access to food and water.", "Software is set to collect data at selected interv... |
61,833 | Como importar in protocolo existente | 1 | dx.doi.org/10.17504/protocols.io.81wgb65rnlpk/v1 | https://www.protocols.io/view/como-importar-in-protocolo-existente-b8mhru36 | Nastia Malochka, Gabriel Gasque | TITLE: Como importar in protocolo existente
AUTHORS: Nastia Malochka, Gabriel Gasque
[DESCRIPTION]
1 Send us your protocol.
2 We enter and check your protocol.
Our editorial team will enter the protocol and double check it to make sure there are no errors or typos relative to the document that you provided.
3 Re... | ["Si desea enviar un protocolo existente a protocols.io, visite primero la siguiente pagina de internet: https://www.protocols.io/we-enter-protocols", "En esta pagina, suba el protocolo que desea que nosotros importemos, a través de la ventana que se indica en la figura y que puede encontrar abajo en la página:", "Pued... |
32,295 | Protocol for the systematic review of the efficacy of the fixed combination of latanoprost and timolol versus other fixed combinations for primary open-angle glaucoma and ocular hypertension | null | dx.doi.org/10.17504/protocols.io.bbsfinbn | null | Shaohua Huang, Yi Xing, Lijuan Zhu, Ke Zhang | TITLE: Protocol for the systematic review of the efficacy of the fixed combination of latanoprost and timolol versus other fixed combinations for primary open-angle glaucoma and ocular hypertension
AUTHORS: Shaohua Huang, Yi Xing, Lijuan Zhu, Ke Zhang
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"... | [] |
29,023 | Lambda Fague Lysis | null | dx.doi.org/10.17504/protocols.io.8j7hurn | null | Laura Sánchez | TITLE: Lambda Fague Lysis
AUTHORS: Laura Sánchez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Lambda phage is a bacteriophage that infect Gam- bacterias, such as E.coli, trough the outer membrane protein LamB. We use the Lambda phage to characterize if the AEGIS's display system has been properl... | [] |
10,650 | Immune Cell Isolation from Mouse Spleen | null | dx.doi.org/10.17504/protocols.io.nm2dc8e | null | Grace Burgin, Noga Rogel, Moshe Biton | TITLE: Immune Cell Isolation from Mouse Spleen
AUTHORS: Grace Burgin, Noga Rogel, Moshe Biton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">To isolate immune cells from a mouse spleen before sorting </div></div>
[STEPS]
?. Prepare 50mL of 10% FBS RPMI media
[FBS]
[RMPI]
**other media may be used
... | ["Prepare 50mL of 10% FBS RPMI media\n[FBS]\n[RMPI]\n**other media may be used", "Wet both sides of a 70μm filter with media above a 50mL conical (make sure there is some media in the conical)", "Place spleen on filter, use the plunger base of a syringe to mash the spleen on to the filter while pouring media through", ... |
21,171 | UC Davis - Temperature and activity by Telemetry | null | dx.doi.org/10.17504/protocols.io.ywtfxen | null | John Rutledge | TITLE: UC Davis - Temperature and activity by Telemetry
AUTHORS: John Rutledge
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary</span></div><div class = "text-block">Utilizing the TA-F10 (DSI) telemeter we can simultaneously collect temperature and activity m... | ["Mice are anesthetized with isoflurane (2.5%) and the flank/back was shaved and disinfected.", "Implantation of transmitter subcutaneously along the right flank.a. Through the same ventral neck incision, a subcutaneous pouch is formed for placementof the transmitter body along the animal’s right flank.b. Using a pair ... |
54,696 | Janelia Atalanta series plasmid cloning | 4 | dx.doi.org/10.17504/protocols.io.bp2l6bjokgqe/v1 | https://www.protocols.click/view/janelia-atalanta-series-plasmid-cloning-bzngp5bw | David Stern | TITLE: Janelia Atalanta series plasmid cloning
AUTHORS: David Stern
[DESCRIPTION]
The Janelia Atalanta plasmids allows simple cloning of a gRNA and homology arms for CRISPR/Cas9 mediated homology directed repair in Drosophila. Gateway-compatible arms are synthesized with appropriate attL recognition sequences and homo... | ["[Make plasmid DNA] Transform the pJAT into ccdB Survival cells following the standard protocol. Plate cells on LB _ Ampicillin (100ug/mL) and grow at 37°C at least 15 hours.", "[Make plasmid DNA] Pick one colony and grow in 3mL LB + antibiotics. I normally grow in Ampicillin, but the plasmid encodes three antibiotic ... |
51,400 | Immunoblotting | 1 | dx.doi.org/10.17504/protocols.io.bp2l6be9zgqe/v1 | https://www.protocols.io/view/immunoblotting-bwfgpbjw | Will Hancock-Cerutti, Pietro De Camilli | TITLE: Immunoblotting
AUTHORS: Will Hancock-Cerutti, Pietro De Camilli
[DESCRIPTION]
This protocol describes collection of protein from cultured cells and immunoblotting, including immunoblotting of large proteins using a proprietary tris-acetate buffer system.
[STEPS]
SECTION: Cell culture and treatments
1. Culture ... | ["[Cell culture and treatments] Culture the HeLa-M cells at 37 °C in 5% CO2 and DMEM containing 10% FBS, 100 U/ml penicillin, 100 mg/mL streptomycin, and 2 Milimolar (mM) L-glutamine (all from Gibco).", "[Cell culture and treatments] For any given experiment, plate the cells at such density so as to be approximately 90... |
null | null | null | dx.doi.org/10.17504/protocols.io.rcad2se | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol was used to generate a single cell suspension from adult human lung tissue. The procedure is carried out on ice, reducing artifact gene expression changes. </p>
[GUIDELINES]
<div>
<p><strong>Enzyme Mixes</strong></p>
<p> </p>
<p><strong>Coll. A/Elastase/Dispase... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.eknbcve | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Transformation of heat-shock competent E. coli cells
[BEFORE_START]
For incubation on ice, make sure the tubes are standing in an ice-water mix, because without water, the cooling effect of ice is not reproducible due to the air between the ice fragments, especially if you have... | [] |
43,896 | DAb-seq: Single-Cell DNA and Antibody Sequencing | 4 | dx.doi.org/10.17504/protocols.io.bn4ymgxw | https://www.protocols.io/view/dab-seq-single-cell-dna-and-antibody-sequencing-bn4ymgxw | Benjamin Demaree, Cyrille Delley, Harish N. Vasudevan, Cheryl A.C. Peretz, David Ruff, Catherine C. Smith, Adam R Abate | TITLE: DAb-seq: Single-Cell DNA and Antibody Sequencing
AUTHORS: Benjamin Demaree, Cyrille Delley, Harish N. Vasudevan, Cheryl A.C. Peretz, David Ruff, Catherine C. Smith, Adam R Abate
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Studies of acute myeloid leukemia rely on DNA sequencing and immuno... | ["[Conjugation of antibodies to oligonucleotide barcodes]\nResuspend each monoclonal antibody to in PBS.\n100 µg\n100 µl\nAntibody must be ordered in protein-free, azide-free buffer, preferably plain PBS. Resuspended from lyophilized stock with trace amounts of trehalose (e.g. from R&D Systems) is also acceptable. Fo... |
80,659 | The impact of globalization on oral health in Moorea, French Polynesia | 1 | dx.doi.org/10.17504/protocols.io.n92ldpdbxl5b/v1 | https://www.protocols.io/view/the-impact-of-globalization-on-oral-health-in-moor-csztwf6n | Kenechi Elvis Obiorah | TITLE: The impact of globalization on oral health in Moorea, French Polynesia
AUTHORS: Kenechi Elvis Obiorah
[DESCRIPTION]
A literature and art review of oral health at the time of European contact and throughout written history and a survey on the oral health quality of native Pacific Islanders today to examine t... | ["[A digital collection of art pieces and literature describing/showing oral health quality of Native Pacific Islanders at early contact time.] On a \"food-for-thought truck\" data will be collected during dental consultations by Tahitian dentists and compiled to access the oral health quality of each patient. Cavities... |
22,272 | Mannitol Agar | 1 | null | https://www.protocols.io/view/mannitol-agar-zy8f7zw | Adrien Assie, Buck Samuel | TITLE: Mannitol Agar
AUTHORS: Adrien Assie, Buck Samuel
[DESCRIPTION]
Mannitol agar recipe
[STEPS]
1. Start with 850mL distilled water
2. 3g Peptone
3. 5g Yeast extract
4. 25g Mannitol
5. Bring to 1000mL distilled water
6. (For LB Agar add 15 g agar)
7. Autoclave | ["Start with 850mL distilled water", "3g Peptone", "5g Yeast extract", "25g Mannitol", "Bring to 1000mL distilled water", "(For LB Agar add 15 g agar)", "Autoclave"] |
50,843 | Evaluating large spontaneous deletions in a bovine cell line selected for bovine viral diarrhea virus resistance - MDBK reads mapping to regions in genome not shared with CRIB cell line | 5 | dx.doi.org/10.17504/protocols.io.bvv3n68n | https://www.protocols.io/view/evaluating-large-spontaneous-deletions-in-a-bovin-bvv3n68n | aspen.workman , mike.heaton , Dennis A. Webster, Gregory P Harhay, Tim Smith, Shollie M Falkenberg, Daniel F. Carlson, Tad S Sonstegard, ted.kalbfleisch | TITLE: Evaluating large spontaneous deletions in a bovine cell line selected for bovine viral diarrhea virus resistance - MDBK reads mapping to regions in genome not shared with CRIB cell line
AUTHORS: aspen.workman , mike.heaton , Dennis A. Webster, Gregory P Harhay, Tim Smith, Shollie M Falkenberg, Daniel ... | ["[Check BAM Files] Cell line library BAM files\nIllumina libraries of CRIB and MDBK cell lines\nThe library names are\n\nLIB14393_Bovine_CRIBcells.Bt_ARS-UCD1.2.realigned.bam\nLIB14394_Bovine_MDBKcells.Bt_ARS-UCD1.2.realigned.bam\n\ncheck files \n\n\nLIB14393_Bovine_CRIBcells.Bt_ARS-UCD1.2.realigned.bam\n \n\n\n \n\n\... |
null | null | null | dx.doi.org/10.17504/protocols.io.fu4bnyw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span class="TextRun SCX169215876" lang="EN-US" style="font-size: 14pt; font-family: Times New Roman,serif; line-height: 23px;" xml:lang="EN-US"><span class="NormalTextRun SCX169215876" style="background-color: inherit;">The goal of this protocol is to produce carbon-fiber pr... | [] |
59,137 | PEI Coating | 4 | null | https://www.protocols.io/view/pei-coating-b5y9q7z6 | kat.titterton | TITLE: PEI Coating
AUTHORS: kat.titterton
[DESCRIPTION]
PEI coating 96 and /or 384 wps for highly adherent NGN2 iN replating on DIV3
Based on SOP from Max+ Bobby / Background optimization results
Also refer to: https://www.protocols.io/view/pei-laminin-coating-b5y8q7zw
[STEPS]
SECTION: PEI Stock Prep (1x)
1. Add ... | ["[PEI Stock Prep (1x)] Add 500 mg of to a 50mL conical tube.", "[PEI Stock Prep (1x)] Add 30 mL of to the tube", "[PEI Stock Prep (1x)] Add to bring volume up to 45 mL. Mix again.", "[PEI Stock Prep (1x)] Filter-sterilize: pour concentrated PEI solution into a 500mL filter flask. \n\nFOR 1x stock final [PEI] =... |
86,213 | Analyzing cellular ATP levels | 4 | dx.doi.org/10.17504/protocols.io.dm6gp3jmjvzp/v1 | https://www.protocols.io/view/analyzing-cellular-atp-levels-cyfdxti6 | Louise Uoselis | TITLE: Analyzing cellular ATP levels
AUTHORS: Louise Uoselis
[DESCRIPTION]
Protocol for analysis of cellular ATP levels using the Promega Mitochondrial ToxGlo Kit.
[STEPS]
SECTION: Day 1
1. Seed cells into white opaque walled 96 well plates in 80 µL of media/well, aiming for a confluency of ~80-90% the next day at th... | ["[Day 1] Seed cells into white opaque walled 96 well plates in 80 µL of media/well, aiming for a confluency of ~80-90% the next day at the time of treatment. Fill all surrounding wells with water to prevent evaporation of the experimental samples. Make sure you seed a DMSO treatment control for each sample collection ... |
80,597 | How does long term exposure to low levels of radiation affect prenatal health in the Marshall Islands? | 1 | dx.doi.org/10.17504/protocols.io.j8nlkwjzwl5r/v1 | https://www.protocols.io/view/how-does-long-term-exposure-to-low-levels-of-radia-csxvwfn6 | slicata | TITLE: How does long term exposure to low levels of radiation affect prenatal health in the Marshall Islands?
AUTHORS: slicata
[DESCRIPTION]
The purpose of this research proposal is to investigate the public health issues inflicted on women and children following the period of nuclear testing in French Polynesia, spec... | ["[Quantitative Data Collection] Obtain birth and health records from individuals living and originating from Northern Marshallese populations (study group) in the Kwajalein Atoll, Enewetak Atoll, Utrik Atoll, Roungelap Atoll, and Bikini Atoll beginning in 1980.", "[Quantitative Data Collection] Obtain birth and health... |
22,211 | FIN-Seq (Frozen Immunolabeled Nuclei Sequencing) | null | dx.doi.org/10.17504/protocols.io.zxbf7in | null | Ryoji Amamoto, Emanuela Zuccaro, Paola Arlotta, Constance L. Cepko | TITLE: FIN-Seq (Frozen Immunolabeled Nuclei Sequencing)
AUTHORS: Ryoji Amamoto, Emanuela Zuccaro, Paola Arlotta, Constance L. Cepko
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Thousands of frozen, archived tissues from postmortem human central nervous system (CNS) are currently available i... | ["[Nuclei Extraction: ~1 hour]\nPrepare all solutions and keep on ice with the Dounce homogenizer.", "[Nuclei Extraction: ~1 hour]\nFill the glass Dounce homogenizer with 1 mL of cold homogenization buffer.", "[Nuclei Extraction: ~1 hour]\nMince the tissue into little pieces and place in 1% PFA for 5 minutes on ice.", ... |
null | null | null | dx.doi.org/10.17504/protocols.io.dzv765 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
The steps describe how to prepare each virus concentrate. There are multiple options for many of the steps; in the case where there is more than one option they are noted in annotations.
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gvnbw5e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 3">
<div class="layoutArea">
<div class="column">
<p>Invitrogen’s patented Syto dyes are cell-permeant cyanine dyes that bind to nucleic acids. Several Syto dyes are available with varying cell permeability, fluorescence enhancement upon binding to ... | ["[Method I. Electrophoretic Staining] {\"blocks\":[{\"key\":\"e41he\",\"text\":\" Dilute the Syto 60 stain 1:1000 in TE buffer, mix well.\\n \\n \\n \",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"8ad22\",\"text\":\" \",\"type\":\"atomic\",\"depth\":0,\"inlin... |
51,674 | Pure CBD Gummies Dr OZ | 3 | dx.doi.org/10.17504/protocols.io.bwp2pdqe | https://www.protocols.io/view/pure-cbd-gummies-dr-oz-bwp2pdqe | health | TITLE: Pure CBD Gummies Dr OZ
AUTHORS: health
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Official Website@>> </span><a href="https://timesofcbd24x7.com/pure-cbd-gummies-dr-oz" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;font... | [] |
51,029 | Sky Islands Pollination Protocol 2021 | 1 | dx.doi.org/10.17504/protocols.io.bv3vn8n6 | https://www.protocols.io/view/sky-islands-pollination-protocol-2021-bv3vn8n6 | Lauren Ponisio | TITLE: Sky Islands Pollination Protocol 2021
AUTHORS: Lauren Ponisio
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Goals:</span></div><div class = "text-block"><ol style = "list-style-type: decimal;"></ol></div><div class = "text-block">a) a single visit from poll... | ["[Supplies]\nIn the field pollination monitoring:Pollination datasheetFruit monitoring datasheetSpecimen datasheet Nylon bag covers5 colored strings/hoops (yellow, green, blue, red, purple)White pin flagsPre numbered stickersSterile screw-top vialsTransect tapeGPS PVC quadrantsVideo camera (gropro-like camera)In the l... |
null | null | null | dx.doi.org/10.17504/protocols.io.gunbwve | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Purpose:</strong> To quantify infectious viruses and evaluate the infectivity of a viral sample.</p>
<p><strong>Summary:</strong> 50 µL of serially-diluted (10<sup>-3</sup> to 10<sup>-10</sup>) virus sample is added to 150 µL of exponentially growing host cells in tri... | [] |
64,439 | Effectiveness of rehabilitation for osteoarthritis of the knee associated with isolated meniscus injury: a scoping review protocol V2 | 1 | dx.doi.org/10.17504/protocols.io.6qpvrd5w3gmk/v2 | https://www.protocols.io/view/effectiveness-of-rehabilitation-for-osteoarthritis-ca6xshfn | Masateru Hayashi, Shusaku Koga, Takashi Kitagawa | TITLE: Effectiveness of rehabilitation for osteoarthritis of the knee associated with isolated meniscus injury: a scoping review protocol V2
AUTHORS: Masateru Hayashi, Shusaku Koga, Takashi Kitagawa
[DESCRIPTION]
⮚ Objective: The purpose of this scoping review is to examine whether there are differences in rehabilit... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kfcctiw | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.r7zd9p6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>1. Draw a circle (c1).</p>
<p>2. Within that circle, about a third of the way from the top draw two points.</p>
<p>3. Another third of the way draw a semicircle (starting at the top and curving down, then back up).</p>
<p>4. from the bottom of the circle extend a line (L1) do... | [] |
28,356 | Frozen Tissue Nuclei Extraction (v2) | null | dx.doi.org/10.17504/protocols.io.7xchpiw | null | Carly Martin, Abdul Abdul, Charles Vanderburg, Naeem Nadaf, Ashley Feirrera, Evan Macosko | TITLE: Frozen Tissue Nuclei Extraction (v2)
AUTHORS: Carly Martin, Abdul Abdul, Charles Vanderburg, Naeem Nadaf, Ashley Feirrera, Evan Macosko
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for extraction of nuclei from frozen tissue in preparation for single-nuclei sequencing (droplet-bas... | ["Make dissociation buffer, 50 mLs per sample:", "Make extraction buffer, 3 mLs per sample: Dissociation Buffer + 1% Kollidon VA64 + 1% Triton X100 + 1:40 RNase-inhibitor", "Prepare equipment: Set out and chill the following equipment/supplies. The instructions below are for one sample.\n ● Cold block dissecting t... |
77,172 | General Microtome Sectioning of Formalin-Fixed Paraffin Embedded (FFPE) blocks | 4 | dx.doi.org/10.17504/protocols.io.bp2l69morlqe/v1 | https://www.protocols.io/view/general-microtome-sectioning-of-formalin-fixed-par-cpkuvkww | Toby J Curless, Zane Jaunmuktane | TITLE: General Microtome Sectioning of Formalin-Fixed Paraffin Embedded (FFPE) blocks
AUTHORS: Toby J Curless, Zane Jaunmuktane
[DESCRIPTION]
This protocol describes the steps for the sectioning of formalin-fixed paraffin embedded blocks in preparation for further tissue processing such as immunohistochemistry.
[BEFO... | ["[Preparation] Check all equipment, consumables and supplies are in place.", "[Sectioning] Once both block and water are at the correct temperature, insert the block into the chuck of the microtome.", "[Sectioning] Adjust the levers on the microtome to position the face of the block parallel with the blade.", "[Prepar... |
93,487 | Hindlimb Scoring | 4 | dx.doi.org/10.17504/protocols.io.n2bvj3mnnlk5/v1 | https://www.protocols.io/view/hindlimb-scoring-c7ipzkdn | Tim Sampson, Ian N Krout | TITLE: Hindlimb Scoring
AUTHORS: Tim Sampson, Ian N Krout
[DESCRIPTION]
This assay details our methodology for examining the hindlimb clasping of mice as a measure for PD like pathology and neurobehavior. Hindlimb clasping has been used in many studies as a measure of PD like progression as the extent of clasping corr... | ["[Acclimation] Bring the mice up from the vivarium to the behavior room at least 1h prior to assessment to allow for acclimation to the environment prior to assessment. Assessment will take place in the home cage, so there is no need for other material.", "[Set-up] Assessment will take place in the home cage, so very ... |
107,617 | Intracellular recording and dye filling of human myenteric neurons | 0 | dx.doi.org/10.17504/protocols.io.3byl49yn2go5/v1 | https://www.protocols.io/view/intracellular-recording-and-dye-filling-of-human-m-dmb942r6 | Wai Ping Yew, Simon JH Brookes | TITLE: Intracellular recording and dye filling of human myenteric neurons
AUTHORS: Wai Ping Yew, Simon JH Brookes
[DESCRIPTION]
This protocol explains how to make intracellular recordings and dye fills with glass micropipettes filled with potassium chloride and 5,6 carboxyfluorescein. It also includes immunohistochemi... | ["[Collecting tissue] Handling all of un-fixed human tissue must be exclusively done by staff trained in occupational health and safety requirements for handling hazardous material, wearing appropriate PPE (personal protective equipment) (gloves, gowns and masks) and working in areas designated for human tissue, with a... |
79,873 | Western Blot Analysis | 1 | dx.doi.org/10.17504/protocols.io.j8nlkwpy5l5r/v1 | https://www.protocols.io/view/western-blot-analysis-cr89v9z6 | michela.deleidi, Federico Bertoli | TITLE: Western Blot Analysis
AUTHORS: michela.deleidi, Federico Bertoli
[DESCRIPTION]
Western blot protocol
[STEPS]
1. Wash cells 1X in PBS.
2. Detach cells using Accutase for 5 minutes at 37°C and collect them.
3. Spin cells in a centrifuge at 250g for 5 minutes at room temperature.
4. Remove the supernatant.
5. ... | ["Wash cells 1X in PBS.", "Detach cells using Accutase for 5 minutes at 37°C and collect them.", "Spin cells in a centrifuge at 250g for 5 minutes at room temperature.", "Remove the supernatant.", "Lyse cells in 1% TBS + 0.5% NP40 + PI/PHI (Pierce #A32959).", "Determine protein concentration was determined using the Pi... |
54,966 | Prokaryotes 16S-V4V5 rRNA Metabarcoding PCR protocol for NGS Illumina sequencing | 4 | dx.doi.org/10.17504/protocols.io.bzwwp7fe | https://www.protocols.io/view/prokaryotes-16s-v4v5-rrna-metabarcoding-pcr-protoc-bzwwp7fe | Sarah Romac | TITLE: Prokaryotes 16S-V4V5 rRNA Metabarcoding PCR protocol for NGS Illumina sequencing
AUTHORS: Sarah Romac
[DESCRIPTION]
Nowadays metabarcoding approaches allow to explore the diversity of different communities using next-generation sequencing (NGS).
Here we describe the 16S-V4V5 DNA amplification method applied f... | ["[Tagged Primer Design and preparation] We use the prokaryotic 16SV4V5 primer set 515fY- 926r from Parada et al. 2016.", "[Tagged Primer Design and preparation]", "[Tagged Primer Design and preparation] Lyophilized Tagged-primers are obtained at Eurogentec, using the RP-Cartridge Gold purification.\n\nWork always unde... |
27,996 | Plasmid Isolation: Miniprep (Protocol for GeneJET Plasmid Miniprep Kit) | null | dx.doi.org/10.17504/protocols.io.7j4hkqw | null | Alba Balletbó | TITLE: Plasmid Isolation: Miniprep (Protocol for GeneJET Plasmid Miniprep Kit)
AUTHORS: Alba Balletbó
[STEPS]
?. Centrifuge overnight culture at 4700rpm for 5 minutes and discard the supernatant.
?. Add 250 µL Resuspension solution and resuspend the cells in this. Transfer the mix to an Eppendorf tube.
?. Add 250 µL L... | ["Centrifuge overnight culture at 4700rpm for 5 minutes and discard the supernatant.", "Add 250 µL Resuspension solution and resuspend the cells in this. Transfer the mix to an Eppendorf tube.", "Add 250 µL Lysis solution and invert the tube 4-6 times.", "Add 350 µL Neutralization solution and invert the tube 4-6 times... |
92,041 | Protocolo Cuidados e Gerenciamento em Analgesia do Parto Normal | 1 | dx.doi.org/10.17504/protocols.io.3byl4q142vo5/v1 | https://www.protocols.io/view/protocolo-cuidados-e-gerenciamento-em-analgesia-do-c55hy836 | Robertarmb Caldas, sonia.ferreira, barbara.lima | TITLE: Protocolo Cuidados e Gerenciamento em Analgesia do Parto Normal
AUTHORS: Robertarmb Caldas, sonia.ferreira, barbara.lima
[DESCRIPTION]
Este Protocolo tem a finalidade de auxiliar médicos anestesiologistas e outros profissionais da saúde que presta, assistência às gestantes em trabalho de parto com o objetivo d... | ["[A elaboração do Protocolo Cuidados e Gerenciamento em Analgesia do Parto Normal] Inicialmente foi feita a definição do escopo, tema e construção da pergunta da pesquisa. Para o estudo da viabilidade da elaboração, validação e futura implantação de um protocolo de analgesia de parto no referido hospital, foi realizad... |
86,305 | A universal protocol for high-quality DNA and RNA isolation from diverse plant species | 4 | dx.doi.org/10.17504/protocols.io.8epv5xj86g1b/v1 | https://www.protocols.io/view/a-universal-protocol-for-high-quality-dna-and-rna-cyh9xt96 | Farhad Masoomi-Aladizgeh, Leila Jabbari, Reza Khayam Nekouei, Ali Aalami, Brian J Atwell, Paul A Haynes | TITLE: A universal protocol for high-quality DNA and RNA isolation from diverse plant species
AUTHORS: Farhad Masoomi-Aladizgeh, Leila Jabbari, Reza Khayam Nekouei, Ali Aalami, Brian J Atwell, Paul A Haynes
[DESCRIPTION]
Next-generation sequencing demands high-quality nucleic acid, yet isolating DNA and RNA from plant... | ["[Lysis Buffer for DNA and RNA Isolation] The lysis buffer contains 0.5% CTAB, 1% EDTA, 2.5% Tris base and 5% NaCl. These are the four main components of the lysis buffer for DNA and RNA isolation from plant tissues. \n\nExample: Add CTAB (125 mg), EDTA (250 mg), Tris base (625 mg), and NaCl (1250 mg) to 25 ml nucleas... |
41,877 | Qualitative & Quantitative Assessment of Human Islets for Distribution Using Dithizone (DTZ) | 1 | dx.doi.org/10.17504/protocols.io.bk5vky66 | https://www.protocols.io/view/qualitative-quantitative-assessment-of-human-islet-bk5vky66 | Integrated Islet Distribution Program | TITLE: Qualitative & Quantitative Assessment of Human Islets for Distribution Using Dithizone (DTZ)
AUTHORS: Integrated Islet Distribution Program
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">This Standard Operating Procedure is adapted from the work of the '</sp... | ["[Preparation of Working Dithizone]\nAssemble all items described in the Materials section.", "[Preparation of Working Dithizone]\nPrepare DTZ stain as described below. Observe all safety precautions when working with DMSO.", "[Preparation of Working Dithizone]\nDissolve dithizone in DMSO.\n50 mg\n10 mL", "[Preparat... |
11,412 | Chelex DNA isolation for quick plant genotyping | null | dx.doi.org/10.17504/protocols.io.pdudi6w | null | Magdalena Julkowska | TITLE: Chelex DNA isolation for quick plant genotyping
AUTHORS: Magdalena Julkowska
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Quick and dirty, but very very fast protocol for DNA isolation. It works beautifully if you have plenty of lines to genotype - example T-DNA insertion lines for validat... | ["Grind tissue in liquid N2 (1 leaf should be enough)If you collect the tissue in a tube containing glass beads (1-2 mm diameter) you can put the frozen tissue samples in the tissue grinder. This is by far the most efficient method to gind large quantities of various samples.", "Add 200μl 10% Chelex (in MilliQ)", "Vort... |
20,620 | Case - Glycerol and Glucose Assays by GC-mass spectrometry | null | dx.doi.org/10.17504/protocols.io.ydkfs4w | null | Henri Brunengraber | TITLE: Case - Glycerol and Glucose Assays by GC-mass spectrometry
AUTHORS: Henri Brunengraber
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block"><span>Total glycerol is determined by first hydrolyzing glycerides (in plasm... | ["Total / **Free Glycerol AssayProtocol:1. Place 20μl of plasma sample or 50 mg tissue into glass, screw-top tube 2. Add ~20μl of IS [2H5]glycerol, for concentration measurements 3. For total glycerol: add 200μl of KOH-EtOH (1M KOH in 70% ethanol) 4. Heat at 80ºC for 3 hours on a heating block to hydrolyze glyceride es... |
81,695 | 5. Taxon Group: Chitons | 4 | dx.doi.org/10.17504/protocols.io.eq2ly7krmlx9/v1 | https://www.protocols.io/view/5-taxon-group-chitons-ctz7wp9n | Kesella Scott-Somme, Inez Januszczak | TITLE: 5. Taxon Group: Chitons
AUTHORS: Kesella Scott-Somme, Inez Januszczak
[DESCRIPTION]
This is part of the collection "DToL Taxon-specific Standard Operating Procedures (SOPs) for Marine Metazoa", lead by the Other Metazoa Working Group. The SOP collection contains guidance on how to process the various marine Met... | [] |
54,239 | Titan Illumina PE SARS-CoV-2 Strain Characterization Workflow for the Terra Platform | 5 | null | https://www.protocols.io/view/titan-illumina-pe-sars-cov-2-strain-characterizati-by77pzrn | Jill V Hagey, Kevin Libuit, Frank J Ambrosio, Technical Outreach and Assistance for States Team | TITLE: Titan Illumina PE SARS-CoV-2 Strain Characterization Workflow for the Terra Platform
AUTHORS: Jill V Hagey, Kevin Libuit, Frank J Ambrosio, Technical Outreach and Assistance for States Team
[DESCRIPTION]
The Titan_Illumina_PE workflow is a part of the Public Health Viral Genomics Titan series for SARS-CoV-2 g... | ["[Setup Terra and Google Cloud Accounts]", "[Import Titan Illumina PE workflow from Dockstore] Importing the Titan Workflow from Dockstore to the User Workspace\n\nWe will first walk through step by step how to import the Titan workflow and at the end there is a video showing the full process.\n\nFirst create a new wo... |
null | null | null | dx.doi.org/10.17504/protocols.io.rr3d58n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Protocol for heatshock transforming E.coli competent cells, from strains such as DH5-alpha. These can be made competent in-house or bought from various suppliers.</p>
<p> </p>
<p>Transformations are essential to using the DNA Distribution Kits: resuspend the DNA sample in a w... | [] |
28,355 | Protocol to culture mESCs (LSCB, UPLUT) | 1 | dx.doi.org/10.17504/protocols.io.7xbhpin | https://www.protocols.io/view/protocol-to-culture-mescs-lscb-uplut-7xbhpin | Stefano Vianello, Mehmet Girgin, Giuliana Rossi, Matthias Lutolf | TITLE: Protocol to culture mESCs (LSCB, UPLUT)
AUTHORS: Stefano Vianello, Mehmet Girgin, Giuliana Rossi, Matthias Lutolf
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Standard protocol used for culturing mouse embryonic stem cells (mESCs) in the Lutolf Lab, EPFL.</div></div>
[STEPS]
?. [Cell deta... | ["[Cell detachment]\nCell detachment:", "[Cell detachment]\nUsing a vacuum pump+glass pipette, aspirate all culture medium out of the well (but do not dry out the cells completely!)", "[Cell detachment]\nAdd 2mL PBS-/- (against side of the well) ti wash medium traces, and then aspirate it out again", "[Cell detachment... |
null | null | null | dx.doi.org/10.17504/protocols.io.p43dqyn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol decribes how to make 1 L of Luria-Bertani agar (also known as LB agar) for the culturing of bacteria on plates. </p>
[GUIDELINES]
<p>1 L of LB agar makes ~20 plates. </p>
<p>At 4 °C unused plates can be stored for around 2 weeks prior to use.</p>
<p>Once used, ... | [] |
41,803 | Oceanit Lateral Flow Assay (LFA) Protocol | 1 | dx.doi.org/10.17504/protocols.io.bk3jkykn | https://www.protocols.io/view/oceanit-lateral-flow-assay-lfa-protocol-bk3jkykn | proposals , Dexter Poon | TITLE: Oceanit Lateral Flow Assay (LFA) Protocol
AUTHORS: proposals , Dexter Poon
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This experiment uses infectious WA1 strain of SARS-CoV2 and is conducted in a qualified BSL3 facility at the University of Hawaii John A Burns School of Medicine (Honolul... | ["[Oceanit Lateral Flow Assay (LFA) Protocol]\nPrepare Serial DilutionThis experiment uses infectious WA1 strain of SARS-CoV2 and is conducted in a qualified BSL3 facility at the University of Hawaii John A Burns School of Medicine (Honolulu, Hawaii).", "[Oceanit Lateral Flow Assay (LFA) Protocol]\nAliquot diluent solu... |
57,792 | Mitomycin C inactivation of mouse embryonic fibroblasts (MEFs) for hPSC cultures | 4 | dx.doi.org/10.17504/protocols.io.b4n8qvhw | https://www.protocols.io/view/mitomycin-c-inactivation-of-mouse-embryonic-fibrob-b4n8qvhw | Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner | TITLE: Mitomycin C inactivation of mouse embryonic fibroblasts (MEFs) for hPSC cultures
AUTHORS: Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This protocol describes the process of using Mitomycin C to inactivate mouse embryonic fibroblasts (MEFs), which can then be used as fe... | ["Grow MEFs to 90-95% confluency in 15-cm cell culture dish", "Aspirate the medium and add 15 ml of Mitomycin C solution to cover the surface", "Incubate in Mitomycin C for 150 min 37 °C", "Aspirate Mitomycin C solution from the plates and wash 4 times with DPBS (with Ca/Mg) and final wash with DPBS (w/o Ca/Mg).", "Add... |
91,708 | Hematoxylin and Eosin Stain - FOR PARAFFIN-EMBEDDED TISSUE SECTIONS | 1 | dx.doi.org/10.17504/protocols.io.kxygx3q9zg8j/v1 | https://www.protocols.io/view/hematoxylin-and-eosin-stain-for-paraffin-embedded-c5s4y6gw | Michael X. Henderson | TITLE: Hematoxylin and Eosin Stain - FOR PARAFFIN-EMBEDDED TISSUE SECTIONS
AUTHORS: Michael X. Henderson
[DESCRIPTION]
This protocol details Hematoxylin and Eosin Stain for paraffin-embedded tissue sections.
[GUIDELINES]
Results:
1. Nuclei are stained blue.
2. Cytoplasm stained various shades of pink, identifying di... | ["[Hematoxylin and Eosin Stain] Deparaffinize slides and hydrate to ddH2O:", "[Hematoxylin and Eosin Stain] Filter undiluted Harris Hematoxylin and Eosin using Whatman #4 filter paper.", "[Hematoxylin and Eosin Stain] Immerse sections in filtered Harris Hematoxylin for 5 min.", "[Hematoxylin and Eosin Stain] Rinse in 2... |
33,962 | Extracting shape and size information from fungal spores | 1 | dx.doi.org/10.17504/protocols.io.bdeii3ce | https://www.protocols.io/view/extracting-shape-and-size-information-from-fungal-bdeii3ce | Alexander Ordynets, Sarah Keßler, Christina Willemsens | TITLE: Extracting shape and size information from fungal spores
AUTHORS: Alexander Ordynets, Sarah Keßler, Christina Willemsens
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The protocol explains extracting and quantifying the shape and size information from the fungal spores. The complex informat... | ["[SHAPE: ChainCoder]\nSelect files containing all pictures for one specimen. They are BMPs located in their own folder, according to the instructions above.Then proceed the furtherpictures one-by-one.Click \"Load Image\".Click 'Select Area' to crop the image and leave just the area of interest.", "[SHAPE: ChainCoder]\... |
50,570 | Parsing of OAI_DC metadata in OpenRefine from OJS articles | 1 | dx.doi.org/10.17504/protocols.io.bvmin44e | https://www.protocols.io/view/parsing-of-oai-dc-metadata-in-openrefine-from-ojs-bvmin44e | Alessandra Moi, carlo.bianchini, Andrea Marchitelli | TITLE: Parsing of OAI_DC metadata in OpenRefine from OJS articles
AUTHORS: Alessandra Moi, carlo.bianchini, Andrea Marchitelli
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Abstract</div><div class = "text-block">Protocols for parsing metadata in OAI_DC format of articles harvested from Open Jo... | ["[Attività preliminari]\nImport the OAI identifiers and generate the baseurl list:", "[Attività preliminari]\nImport the identifiers following the instructions in the protocolhttps://www.protocols.io/view/come-importare-identificatori-oai-pmh-in-openrefin-bp7mmrk6", "[Attività preliminari]\nFrom the list of imported i... |
51,310 | COVID-19: Detecting Indirect Spread in Facilities for Enhanced Care sTudy (COVID-19: DISinFECT). Investigating environmental epidemiology of SARS-CoV-2 in long term care facilities in England. V4.1. | 1 | dx.doi.org/10.17504/protocols.io.bwcnpave | https://www.protocols.io/view/covid-19-detecting-indirect-spread-in-facilities-f-bwcnpave | rachel.kwiatkowska , Ginny Moore, Allan Bennett, Nicola Love, Matthew Donati, Roberto Vivancos, Derren Ready | TITLE: COVID-19: Detecting Indirect Spread in Facilities for Enhanced Care sTudy (COVID-19: DISinFECT). Investigating environmental epidemiology of SARS-CoV-2 in long term care facilities in England. V4.1.
AUTHORS: rachel.kwiatkowska , Ginny Moore, Allan Bennett, Nicola Love, Matthew Donati, Roberto Vivancos, Derren Re... | ["[Introduction]\nGlossaryCOVID-19 – Coronavirus Disease 2019COG-UK - COVID-19 Genomics UK consortiumCPHI – Consultant in Public Health InfectionIPC – Infection Prevention & ControlFS – Field (Epidemiology) ServiceMERS-CoV – Middle East Respiratory Syndrome CoronavirusNBT – North Bristol NHS Foundation TrustNHS – Natio... |
76,469 | Protocol for Making Data Publicly Available in USDA-ARS | 1 | dx.doi.org/10.17504/protocols.io.ewov1o5bolr2/v1 | https://www.protocols.io/view/protocol-for-making-data-publicly-available-in-usd-cnwvvfe6 | Eric Billman, Clement Sohoulande, Matias Vanotti | TITLE: Protocol for Making Data Publicly Available in USDA-ARS
AUTHORS: Eric Billman, Clement Sohoulande, Matias Vanotti
[DESCRIPTION]
This SOP was created to comply with the new P&P 630.0 Data Management (usda.gov) ‘Data Management & Public Access Requirements for ARS’ which affects all journal papers produc... | ["[Protocol for Making Data Publicly Available] Protocol for Making Data Publicly Available in USDA-ARS\n\nDeveloped by: Drs. Eric Billman, Clement Sohoulande, and Matias Vanotti\nUSDA-ARS, Florence, SC\n\nObjective:\nThis SOP was created to comply with the new P&P 630.0 Data Management (usda.gov) ‘Data Management & Pu... |
48,662 | Operating Procedures: Ring Infiltrometer | 1 | null | https://www.protocols.io/view/operating-procedures-ring-infiltrometer-btrwnm7e | USDA Usda | TITLE: Operating Procedures: Ring Infiltrometer
AUTHORS: USDA Usda
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Operating procedure for using the ring infiltrometer.</div></div>
[STEPS]
?. Fill up your containers with water. Make sure that the water that will be going into the mariotte device ha... | ["Fill up your containers with water. Make sure that the water that will be going into the mariotte device has as little dissolved gas in it as possible.Well water is often supersaturated with CO2, and when the gas bubbles come out of solution they can interfere with flow from the Mariotte to the infiltrometer ring. (S... |
null | null | null | dx.doi.org/10.17504/protocols.io.tkyekxw | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.fjjbkkn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Before starting, please visit the <a href="http://earthcube.org/group/ecogeo" target="_blank">ECOGEO website</a> for more information on this 'Introduction to Environmental 'Omics' training series. The site contains a pre-packaged virtual machine (developed by <a href="https:... | [] |
94,367 | 12S rRNA Environmental DNA Processing Pipeline with size selection | 2 | null | https://www.protocols.io/view/12s-rrna-environmental-dna-processing-pipeline-wit-c8d7zs9n | Kathleen Pitz, Jacoby Baker | TITLE: 12S rRNA Environmental DNA Processing Pipeline with size selection
AUTHORS: Kathleen Pitz, Jacoby Baker
[DESCRIPTION]
Protocol chain from extraction to sequencing for amplification of the 12S rRNA gene targeting bony fish in seawater environmental DNA samples.
[STEPS] | [] |
20,536 | Genomic Editing: iPSC | null | dx.doi.org/10.17504/protocols.io.yayfsfw | null | Celeste Karch, Rita Martinez, Jacob Marsh | TITLE: Genomic Editing: iPSC
AUTHORS: Celeste Karch, Rita Martinez, Jacob Marsh
[STEPS] | [] |
71,930 | NanoLuciferase Assay | 4 | null | https://www.protocols.io/view/nanoluciferase-assay-cig2ubye | afedoriouk, Morgan Farrell, Nicholas J Shikuma | TITLE: NanoLuciferase Assay
AUTHORS: afedoriouk, Morgan Farrell, Nicholas J Shikuma
[DESCRIPTION]
Protocol for a luciferase assay to quantify promoter expression in marine bacteria.
[STEPS]
SECTION: Day 1: Streak out strains
1. Streak out all strains from frozen stock onto the appropriate antibiotic plates. Includin... | ["[Day 1: Streak out strains] Streak out all strains from frozen stock onto the appropriate antibiotic plates. Including a positive and negative control.\nPseudoalteromonas luteoviolacea expressing kanamycin resistant backbone was struck onto NSWT with 300 µg/mL of kanamycin\nPseudoalteromonas sp. PS5 expressing kanamy... |
63,323 | K1 keto Reviews Buy Now? | 1 | dx.doi.org/10.17504/protocols.io.eq2lyn1oevx9/v1 | https://www.protocols.io/view/k1-keto-reviews-buy-now-b933r8qn | zellinavega | TITLE: K1 keto Reviews Buy Now?
AUTHORS: zellinavega
[DESCRIPTION]
One of the most significant concerns facing developed countries today is the prevalence of overweight and obesity. Development countries aren't far behind the developed ones. When a person weighs more than what is thought normal for their height, tha... | ["[K1 keto Reviews Buy Now?]"] |
null | null | null | dx.doi.org/10.17504/protocols.io.hjvb4n6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<p>This protocol is from 'Flower Ca<sup>2+</sup> channel in CME and ADBE' of Yao CK et al.</p>
<div>
<div>
<p> </p>
<p>Please see the manuscript for the full method details.</p>
</div>
</div>
</div>
[BEFORE_START]
<p>You'll need: </p>
<p> </p>
<p><strong>0 mM Ca<sup>2+</s... | [] |
57,798 | Preparing mRNA for nucleofection of hPSCs | 4 | dx.doi.org/10.17504/protocols.io.b4peqvje | https://www.protocols.io/view/preparing-mrna-for-nucleofection-of-hpscs-b4peqvje | Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner | TITLE: Preparing mRNA for nucleofection of hPSCs
AUTHORS: Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This protocol describes the standard procedure for preparing mRNA to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.
General notes
1. Throughout ... | ["Thaw mRNA and synthetic pegRNA/ngRNA on ice", "In each nucleofection, use 4 µg total of mRNA", "For prime editing PE2 strategy, use:\n 4 µg, IVT PE2 mRNA\n 1.5 µl, 100 µM Synthetic pegRNA", "For prime editing PE3 strategy, use:\n 4 µg, IVT PE2 mRNA\n 1 µl, 100 µM Synthetic pegRNA\n 0.5 µl, 100 µM, Synt... |
null | null | null | dx.doi.org/10.17504/protocols.io.d2t8em | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Ultracentrifugation provides a means to concentrate, analyze, and purify viruses in solution, and therefore represents an invaluable tool for aquatic virologists. This protocol provides a method for purification of viruses from culture lysates from natural water samples.
[GUIDE... | [] |
44,162 | NEBNext Single Cell/ Low Input RNA Library Prep Kit for Illumina Protocol for Low Input RNA E6420 | 4 | dx.doi.org/10.17504/protocols.io.e6nvw5k49vmk/v1 | https://www.protocols.io/view/nebnext-single-cell-low-input-rna-library-prep-kit-bpdami2e | New England Biolabs | TITLE: NEBNext Single Cell/ Low Input RNA Library Prep Kit for Illumina Protocol for Low Input RNA E6420
AUTHORS: New England Biolabs
[DESCRIPTION]
The NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® uses a template switching method to generate full length cDNAs directly from single cells or 2 pg –... | ["[Sample and Reagents Preparation] Briefly centrifuge the tubes containing NEBNext Single Cell RT Enzyme Mix and Murine RNase Inhibitor to collect solutions to the bottom of the tubes, then place on ice.", "[Sample and Reagents Preparation] Thaw all other frozen components at Room temperature (if the 10X NEBNext Cell ... |
69,354 | Modified NEBNext® VarSkip Short SARS-CoV-2 Enrichment and library prep for Oxford Nanopore Technologies- adapted for wastewater samples | 4 | dx.doi.org/10.17504/protocols.io.3byl4bwervo5/v2 | https://www.protocols.io/view/modified-nebnext-varskip-short-sars-cov-2-enrichme-cfyitpue | Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Chris Grim, Maria Hoffmann | TITLE: Modified NEBNext® VarSkip Short SARS-CoV-2 Enrichment and library prep for Oxford Nanopore Technologies- adapted for wastewater samples
AUTHORS: Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Chris Grim, Maria Hoffmann
[DESCRIPTION]
This protocol details methods for the preparation of SARS-CoV-2 sequen... | ["[Before you start]", "[cDNA Synthesis] Gently mix 10 times by pipetting and spin down the LunaScript RT SuperMix reagents (contains primers). Prepare the cDNA synthesis reaction as described below:", "[cDNA Synthesis] Flick the tube or pipet up and down 10 times to mix followed by a quick spin.", "[cDNA Synthesis] Fo... |
43,709 | His-tag purification | 1 | dx.doi.org/10.17504/protocols.io.bnw5mfg6 | https://www.protocols.io/view/his-tag-purification-bnw5mfg6 | Andreea S | TITLE: His-tag purification
AUTHORS: Andreea S
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">His tag purification uses the technique of immobilised metal affinity chromatography. In this technique, transition metal ions are immobilized on a resin matrix using a chelating agent such as iminodiacet... | ["[Separate proteins from soil matrix]\nCollect soil samples of\n5 g", "[Separate proteins from soil matrix]\nExtract total proteins using NoviPure Soil Protein Kit or other comercially available kits for total protein soil extraction", "[His-tag separation of NLP]\nWash the Ni2+-sepharose column material with 12 CVs ... |
81,465 | Whole-cell Patch-Clamp Recordings from Striatal Cholinergic Interneurons in ex vivo Mouse Brain Slices | 4 | dx.doi.org/10.17504/protocols.io.3byl4jo5zlo5/v1 | https://www.protocols.click/view/whole-cell-patch-clamp-recordings-from-striatal-ch-ctszwnf6 | Jeffrey Stedehouder, Stephanie J Cragg | TITLE: Whole-cell Patch-Clamp Recordings from Striatal Cholinergic Interneurons in ex vivo Mouse Brain Slices
AUTHORS: Jeffrey Stedehouder, Stephanie J Cragg
[DESCRIPTION]
This protocol describes the steps to perform whole-cell patch-clamp recordings of striatal cholinergic interneurons (ChIs) previously labelled wi... | ["[Injection of mCherry virus to label cholinergic interneurons] Inject AAV5-hSyn-DIO-mCherry (~ 1.3 x 1013 genome copies/mL,) of equivalent AAV bilaterally or unilaterally into the Caudate Putamen (CPu) (ML ±1.75 mm from bregma, AP +0.8 mm from bregma, DV -2.4 mm from brain surface) or Nucleus Accumbens core (NAc) (M... |
null | null | null | dx.doi.org/10.17504/protocols.io.e9tbh6n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="view-protocol-title vpt-block">
<p>The Non-Interfering™ Protein Assay is a colorimetric assay for determining protein concentrations in protein loading buffer (Laemmli buffer), high β-mercaptoethanol concentrations, and in lipid and vesicle preparations.</p>
</div>
... | [] |
27,082 | OSIP 2019 exercice | null | dx.doi.org/10.17504/protocols.io.6pihdke | null | Mathilde Panes, Francesco Varrato | TITLE: OSIP 2019 exercice
AUTHORS: Mathilde Panes, Francesco Varrato
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is a use case for the Open Science In Practice Summer School.</div></div>
[STEPS]
?. [Preparation]
?.
?. | ["[Preparation]"] |
80,972 | Automatic flow in fluid-walled dumbbells driven by Laplace pressure | 1 | dx.doi.org/10.17504/protocols.io.bp2l695m5lqe/v1 | https://www.protocols.io/view/automatic-flow-in-fluid-walled-dumbbells-driven-by-ctbkwikw | Quyen Do, Federico Nebuloni, Richard Wade-Martins | TITLE: Automatic flow in fluid-walled dumbbells driven by Laplace pressure
AUTHORS: Quyen Do, Federico Nebuloni, Richard Wade-Martins
[DESCRIPTION]
This protocol describes experiments performed to quantify pressure and volume variations inside fluid-walled dumbbells when a pressure difference is generated between the ... | ["[Jet-Printing of Fluid-Walled Dumbbells] Fill a virgin uniwell plate with 5 ml of DMEM supplemented with 10% FBS. Agitate the plate to spread the 5 mL of medium to cover the whole area of the plate.", "[Jet-Printing of Fluid-Walled Dumbbells] Remove the volume leaving a thin layer of medium wetting the plate.", "[Jet... |
61,935 | PROTAC Design | 6 | dx.doi.org/10.17504/protocols.io.n92ldz72nv5b/v1 | https://www.protocols.io/view/protac-design-b8qprvvn | boc.protac | TITLE: PROTAC Design
AUTHORS: boc.protac
[DESCRIPTION]
PROTAC (protein degradation targeted chimera) is a special protein degradation technology, which uses ubiquitin proteasome pathway, a natural protein degradation pathway in cells, to remove specific proteins that need to be degraded. A PROTAC molecule consists o... | [] |
57,312 | Immunohistochemistry in wholemounts and cryostat sections in rat stomach | 1 | dx.doi.org/10.17504/protocols.io.yxmvmnk5og3p/v1 | https://www.protocols.io/view/immunohistochemistry-in-wholemounts-and-cryostat-s-b378qrrw | Madeleine Di Natale, Jamie JL Liew, Billie Hunne, Martin Stebbing, John Furness | TITLE: Immunohistochemistry in wholemounts and cryostat sections in rat stomach
AUTHORS: Madeleine Di Natale, Jamie JL Liew, Billie Hunne, Martin Stebbing, John Furness
[DESCRIPTION]
This protocol describes the methods used to evaluate neuronal target and population density in wholemount preparations and cryostat sect... | ["[Immunohistochemistry for neuronal NOS in wholemounts and cryostat sections] Tissue preparation\nFresh stomachs were collected from rats that were deeply anesthetised with an intraperitoneal injection of pentobarbital sodium (100mg/kg), placed into PBS (phosphate-buffered saline: 0.15 M NaCl in 0.01 M sodium phosphat... |
null | null | null | dx.doi.org/10.17504/protocols.io.gsubwew | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 2">
<div class="layoutArea">
<div class="column">
<p>This protocol describes a cell viability assay that uses near-infrared fluorescent detection. Sapphire700 Stain is used to determine cell viability by assessing cell membrane integrity, and the as... | ["[Cell Preparation] Grow Jurkat cells in a 75-cm2 cell culture flask with growth medium (RPIM-1640 supple- mented with 10% FBS) using standard cell culture practices. Always make sure that cells are healthy before using them for the experiment.", "[Staurosporine Treatment] {\"blocks\":[{\"key\":\"3kgcm\",\"text\":\" ... |
null | null | null | dx.doi.org/10.17504/protocols.io.e32bgqe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is for Tube-O-DIALYZER™, a mini dialysis system for small sample volumes (20µl‐2.5ml). Follow this protocol for dialysis devices Cat. # <a href="http://www.gbiosciences.com/ResearchProducts/tubeodialyzer-desc.aspx" target="_blank">786‐610 to 786‐624</a>.</p>
[G... | [] |
52,189 | Enumeration and Propagation of Bacteriophage MS-2 | 4 | dx.doi.org/10.17504/protocols.io.bw75phq6 | https://www.protocols.io/view/enumeration-and-propagation-of-bacteriophage-ms-2-bw75phq6 | leili abkar | TITLE: Enumeration and Propagation of Bacteriophage MS-2
AUTHORS: leili abkar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This Protocol guides through setp-by-step enumeration and propagation of MS-2 bacteriophage. It is prepared and adjusted for </span><span style = "font-weight:bold;fon... | ["[Stock Preparation]", "[1\tStock Preparation]", "[Stock Preparation]\nLB Broth Dilution TubesAdd 20 g of LB broth to 1 liter of milli-QHeat and stir until warm and fully dissolvedAutoclave at 121°C for 20 minutesAseptically transfer 900 µL of sterile broth to 1.5 mL microcentrifuge tubes", "[Stock Preparation]\nE.col... |
91,129 | circRNA-producing gene function enrichment analysis | 1 | dx.doi.org/10.17504/protocols.io.8epv5xmj6g1b/v1 | https://www.protocols.io/view/circrna-producing-gene-function-enrichment-analysi-c48zyzx6 | Xianjun Dong | TITLE: circRNA-producing gene function enrichment analysis
AUTHORS: Xianjun Dong
[DESCRIPTION]
This protocol describes the method to perform function enrichment analysis for the circRNA-producing genes.
[STEPS]
1. | [] |
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