id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.e5kbg4w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>ThI high sensitivity miRNA library generation for the Illumina sequencing platform. Our enhanced reagent kit enables the discovery and profiling of small RNAs from a variety of sources including FFPE, exosome, serum, and whole blood. The TailorMix workflow is designed for eas... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.g5sby6e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is a slightly modified version of Thermo Fisher's gateway LR protocol which uses less enzyme and is therefore more economical.</p>
[STEPS]
?.
?.
?.
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?.
?.
?.
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?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.t9per5n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
There is a weath of microscopy-related information online. This document lists the one we know and like.
[STEPS]
?. | ["{\"blocks\":[{\"key\":\"bujq4\",\"text\":\"iBiology Microscopy Course\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"6gnrp\",\"text\":\"https:\\/\\/www.ibiology.org\\/ibioeducation\\/taking-courses\\/ibiology-microscopy-course.html\",\"type\":\"unstyled\",\"de... |
70,936 | Isolation of Neurospora crassa genomic DNA | 3 | dx.doi.org/10.17504/protocols.io.yxmvm2kkbg3p/v1 | https://www.protocols.io/view/isolation-of-neurospora-crassa-genomic-dna-chhyt37w | kdcastillo | TITLE: Isolation of Neurospora crassa genomic DNA
AUTHORS: kdcastillo
[DESCRIPTION]
Here, we describe the isolation of fungal genomic DNA from Neurospora crassa mycelial mats.
[STEPS] | [] |
98,938 | CTAB Extraction from Sample Ground in Liquid Nitrogen | 0 | dx.doi.org/10.17504/protocols.io.e6nvw157zlmk/v1 | https://www.protocols.io/view/ctab-extraction-from-sample-ground-in-liquid-nitro-dcu22wye | Jordan Beasley | TITLE: CTAB Extraction from Sample Ground in Liquid Nitrogen
AUTHORS: Jordan Beasley
[DESCRIPTION]
This protocol describes the process of grinding a sample in liquid nitrogen and extracting DNA with a CTAB extraction.
[STEPS]
SECTION: Cryogenic Grinding and CTAB Extraction
1. Dispense some liquid nitrogen in a small ... | ["[Cryogenic Grinding and CTAB Extraction] Dispense some liquid nitrogen in a small dewer (small thermos size)", "[Cryogenic Grinding and CTAB Extraction] Fill a mortar with liquid nitrogen to cool it and let it boil off. When it has completely boiled away, add a small amount more into the mortar and grind with pestle ... |
73,277 | Plasmid Expansion | 4 | dx.doi.org/10.17504/protocols.io.8epv5jrjjl1b/v1 | https://www.protocols.io/view/plasmid-expansion-cjs5ung6 | Ali Albalakhi, Ning Xia | TITLE: Plasmid Expansion
AUTHORS: Ali Albalakhi, Ning Xia
[DESCRIPTION]
This is the protocol for plasmid expansion.
[STEPS]
1. Thaw the competent cells on ice
2. Chill approximately 5 μg (2 μl) of Plasmid DNA in 1.5 mL microcentrifuge tube
3. Add 50 μl of competent cells to the DNA. Mix gently by pipetting up and dow... | ["Thaw the competent cells on ice", "Chill approximately 5 μg (2 μl) of Plasmid DNA in 1.5 mL microcentrifuge tube", "Add 50 μl of competent cells to the DNA. Mix gently by pipetting up and down or flicking the tube 4–5 times to mix the cells and DNA. Do not vortex.", "Place the mixture on ice for 30 minutes. Do not mi... |
86,730 | Recombinant retroviral expression vectors based on pLXSN that encode EGFR, ERBB2, ERBB3, and ERBB4 | 4 | dx.doi.org/10.17504/protocols.io.261gedd97v47/v1 | https://www.protocols.io/view/recombinant-retroviral-expression-vectors-based-on-cyxixxke | David J Riese II | TITLE: Recombinant retroviral expression vectors based on pLXSN that encode EGFR, ERBB2, ERBB3, and ERBB4
AUTHORS: David J Riese II
[DESCRIPTION]
Recombinant retroviruses are commonly used to direct the ectopic expression of genes in infected cells. There are two significant advantages to this approach. Following inf... | ["[Introduction] Here we describe the construction of pLXSN derivatives that contain the human EGFR [2], ERBB2 [3], ERBB3 [4], and ERBB4 [5] cDNAs. These cDNAs encode receptor tyrosine kinases (RTKs). The EGFR and ERBB2 RTKs are well-validated drivers of many types of human malignancies. However, the roles that the ... |
79,253 | Molecular Observation Network (MONet) | 2 | dx.doi.org/10.17504/protocols.io.5jyl8jr49g2w/v2 | https://www.protocols.io/view/molecular-observation-network-monet-crmvv466 | maggie.bowman, Alexis Heath, Tamas Varga, Anil Battu, Will Kew, Cheng Shi, Rosey Chu, Che Clendinen, Odeta Qafoku, Jason.Toyoda, qian.zhao, izabel.stohel, Rey Hauchambe, Michael Rosenstock, Albert Lawver, nicholas.sconzo, James Anderson, Patricia Miller, andrew.townsend, Nancy Hess, John Bargar, emily.graham | TITLE: Molecular Observation Network (MONet)
AUTHORS: maggie.bowman, Alexis Heath, Tamas Varga, Anil Battu, Will Kew, Cheng Shi, Rosey Chu, Che Clendinen, Odeta Qafoku, Jason.Toyoda, qian.zhao, izabel.stohel, Rey Hauchambe, Michael Rosenstock, Albert Lawver, nicholas.sconzo, James Anderson, Patricia Miller, andrew.town... | [] |
79,548 | A versatile nuclei extraction protocol for single cell multiome ATAC and gene expression in non model species | 4 | dx.doi.org/10.17504/protocols.io.bp2l69wnrlqe/v1 | https://www.protocols.io/view/a-versatile-nuclei-extraction-protocol-for-single-crw4v7gw | Rose Ruiz Daniels, Richard S Taylor, Ioannis Konstantinidis, Sarah Salisbury, Diego Perojil Morata, Jorge Manuel de Oliveira Fernandes, Emily Clark, Dan Macqueen, Diego Robledo | TITLE: A versatile nuclei extraction protocol for single cell multiome ATAC and gene expression in non model species
AUTHORS: Rose Ruiz Daniels, Richard S Taylor, Ioannis Konstantinidis, Sarah Salisbury, Diego Perojil Morata, Jorge Manuel de Oliveira Fernandes, Emily Clark, Dan Macqueen, Diego Robledo
[DESCRIPTION]
H... | ["[Nucleus isolation workflow for ST-based buffers] on ice, place a piece of frozen tissue into one well of a 6-well tissue culture plate with 1 mL TST.", "[Nucleus isolation workflow for ST-based buffers] on ice, mince tissue initially using Tungsten Carbide scissors for 30 s and then with Noyes Spring Scissors for ... |
54,285 | DNA extraction from mouthwash samples | 1 | null | https://www.protocols.io/view/dna-extraction-from-mouthwash-samples-by9mpz46 | Ahmed A Shibl, Anique Ahmad, Tsedenia Denekew, Aashish Jha | TITLE: DNA extraction from mouthwash samples
AUTHORS: Ahmed A Shibl, Anique Ahmad, Tsedenia Denekew, Aashish Jha
[DESCRIPTION]
DNA extraction
[BEFORE_START]
Set centrifuge to 4ºC
Keep CD2 solution on ice
Prepare and label collection tubes, microcentrifuge tubes, and MB spin columns
[STEPS]
1. Thaw mouthwash samples... | ["Thaw mouthwash samples on ice for 30 min", "Transfer desired volume into 1.5 mL or 2 mL eppendorfs\n1-2 mL", "Centrifuge transferred samples at maximum speed at 4 ºC", "Repeat step 1.1 if more volume is needed to see a pellet or a high yield of DNA is required", "Discard the supernatant carefully without disturbing t... |
64,609 | Intraganglionic injection of AAV into nodose ganglia in mice | 1 | dx.doi.org/10.17504/protocols.io.81wgb6w61lpk/v1 | https://www.protocols.io/view/intraganglionic-injection-of-aav-into-nodose-gangl-cbb9sir6 | Seol-Hee Kim, patil, Thomas Taylor-Clark | TITLE: Intraganglionic injection of AAV into nodose ganglia in mice
AUTHORS: Seol-Hee Kim, patil, Thomas Taylor-Clark
[DESCRIPTION]
We have developed a novel method of injecting AAV vectors directly into the sensory ganglia, vagal and dorsal root ganglia, of mice. This technique provides for the first time a tool for ... | ["Approximately 2 cm of incision was made over a shaved superficial portion of the masseter muscle area.", "Mice were anesthetized with a mixture of ketamine (50 mg/kg) and dexmedetomide (0.5 mg/kg) via intraperitoneal injection.", "The vagal nodose ganglia was carefully exposed.", "Micropipettes were filled with AAV (... |
65,033 | Silver Stain Gel for IP-TDMS Development | 1 | null | https://www.protocols.io/view/silver-stain-gel-for-ip-tdms-development-cbrhsm36 | Lauren Adams | TITLE: Silver Stain Gel for IP-TDMS Development
AUTHORS: Lauren Adams
[DESCRIPTION]
Silver stain gels can be used to examine total protein in a given sample. This method is less sensitive than western blotting, but typically more sensitive than Coomassie blue staining.
[STEPS]
1. Take aliquots from IP fractions and... | ["Take aliquots from IP fractions and add sample loading buffer to a final concentration of 1X. Boil samples in hot plate at 95ºC for 5-10 min. Briefly, centrifuge samples to ensure sample entire sample is towards bottom of the tube.", "Open gel and place into gel box. Add 1 x MES running buffer, ensuring that there ar... |
78,159 | Choanoflagellate Ciliogenesis Live Imaging | 4 | dx.doi.org/10.17504/protocols.io.q26g7y9n3gwz/v2 | https://www.protocols.io/view/choanoflagellate-ciliogenesis-live-imaging-cqjpvumn | Maxwell C Coyle | TITLE: Choanoflagellate Ciliogenesis Live Imaging
AUTHORS: Maxwell C Coyle
[DESCRIPTION]
This protocol removes the cilia/flagella from choanoflagellate cells and sets up the cells for live imaging of ciliogenesis. It has been developed for the species Salpingoeca rosetta, but may be portable into other choanoflagellat... | ["[Concentrate cells and remove cilia] Grow choanoflagellate cells (Salpingoeca rosetta fed with Echinicola pacifica, ATCC PRA-390) in High Nutrient Media to a density of 1-2 x 106 cells/ml. Grow at 22 ºC, 60% humidity\n\nWe grow 30 ml of culture in 75 cm2 vented flask. Typically, inoculating this flask with a choanofl... |
87,289 | Protocol of histopathology preparation | 1 | dx.doi.org/10.17504/protocols.io.3byl4q9r2vo5/v1 | https://www.protocols.io/view/protocol-of-histopathology-preparation-czgzx3x6 | Taufik Suryadi . | TITLE: Protocol of histopathology preparation
AUTHORS: Taufik Suryadi .
[DESCRIPTION]
This protocol is a step by step from research entitled: Comparison between pre-mortem histopathological findings in rats with and without traumatic brain injury: prospective application in forensic medicine
[STEPS]
SECTION: Step by ... | ["[Step by step description of histopathology preparation] Each group's tissue was taken for observation on histopathological preparations with a series of manufacturing processes with several stages including euthanasia, fixation, trimming with subsequent stages in the form of dehydration, clarification, paraffin infi... |
39,063 | Cybersecurity Protocols & Resilience Post COVID - Lessons Learnt | 3 | dx.doi.org/10.17504/protocols.io.bidxka7n | https://www.protocols.io/view/cybersecurity-protocols-amp-resilience-post-covid-bidxka7n | lissa coffeey | TITLE: Cybersecurity Protocols & Resilience Post COVID - Lessons Learnt
AUTHORS: lissa coffeey
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.seaebae | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>PNGase F cleaves N-linked (asparagine-linked) oligosaccharides from glycoproteins. The enzyme deaminates asparagine to aspartic acid, leaving the oligosaccharides intact. Denaturation increases the rate of cleavage. Most native proteins can still be completely N-deglycosylate... | [] |
71,938 | HTTM : DNA Extraction | 4 | dx.doi.org/10.17504/protocols.io.q26g7yzz3gwz/v1 | https://www.protocols.io/view/httm-dna-extraction-cihaub2e | Antoine Champie, Amélie De Grandmaison | TITLE: HTTM : DNA Extraction
AUTHORS: Antoine Champie, Amélie De Grandmaison
[DESCRIPTION]
Part two of the HTTM protocol. A low-cost and high-throughput Tn-seq protocol.
This part cover the DNA extraction from cell pellets of transposon insertion mutants and subsequent silica columns regeneration.
[STEPS]
SECTION: ... | ["[DNA extraction] Prepare the lysis solution by adding 165 µL of proteinase K to 66 mL of homemade lysis buffer and mix well.", "[DNA extraction] Add 600 µLof lysis solution to each well of the deep-well plate and resuspend the pellet.", "[DNA extraction] Cover with an adhesive aluminum cover and incubate at 55 °C for... |
98,533 | Functional Characterization of the Human Islet Microvasculature Using Living Pancreas Slices | 4 | dx.doi.org/10.17504/protocols.io.kqdg36n27g25/v2 | https://www.protocols.io/view/functional-characterization-of-the-human-islet-mic-dcgd2ts6 | Luciana Mateus Gonçalves, Joana Almaça | TITLE: Functional Characterization of the Human Islet Microvasculature Using Living Pancreas Slices
AUTHORS: Luciana Mateus Gonçalves, Joana Almaça
[DESCRIPTION]
Pancreatic islets are clusters of endocrine cells that secrete different hormones to regulate blood glucose levels. Efficient hormone secretion requires a cl... | ["[Methods] Human Organ Donors\n\nWe obtained human living pancreas slices from de-identified cadaveric donors (from the head of the pancreas, n = 6 non-diabetic individuals, male and female, ages from 20 – 59 years old; information on the donors used in this study is provided in Table S1) from the Network of Pancreati... |
55,833 | Wastewater Sample Collection - Moore Swab and Grab Sample Methods | 1 | dx.doi.org/10.17504/protocols.io.b2rzqd76 | https://www.protocols.io/view/wastewater-sample-collection-moore-swab-and-grab-s-b2rzqd76 | Jamie VanTassell, Julia Raymond, Marlene K Wolfe, Pengbo Liu, Christine Moe | TITLE: Wastewater Sample Collection - Moore Swab and Grab Sample Methods
AUTHORS: Jamie VanTassell, Julia Raymond, Marlene K Wolfe, Pengbo Liu, Christine Moe
[DESCRIPTION]
This protocol describes materials and methods that can be employed in the field to collect Moore swabs and/or grab samples for wastewater ... | ["[Moore Swab Assembly] Cut pieces of cotton gauze approximately 120 cm long by 15 cm wide.", "[Moore Swab Sample Collection - Day 1] Remove the manhole cover using a manhole cover hook or magnetic manhole lifter.", "[Moore Swab Sample Collection - Day 1] Tie fishing line around a clean Moore swab.", "[Moore Swab Sampl... |
96,697 | Gewebesammlung Frischgewebe Prostatektomie | 1 | null | https://www.protocols.io/view/gewebesammlung-frischgewebe-prostatektomie-danz2df6 | Annika Fendler, Bettina Ergün | TITLE: Gewebesammlung Frischgewebe Prostatektomie
AUTHORS: Annika Fendler, Bettina Ergün
[DESCRIPTION]
Dieses Protokoll beschreibt die Schritte für die Sammlung von Frischgewebe, Gefriergewebe (Fresh-frozen), und Blut von Patienten mit Prostatakarzinom bei Prostatektomie.
Verwandte Dokumente:
Protokoll zur Blutaufa... | ["[Aufarbeitung im Labor] weiter mit Protokoll:", "[Gewebesammlung im OP]", "[Gewebesammlung im OP] Nach Rückmeldung des OPs geht eine Person zur Gewebeentnahme.\nKiste Prostata und Stickstoffbehälter mitnehmen.\nArbeitsanleitung (siehe Abbildung) beachten.", "[Gewebesammlung im OP] 1x 15 ml Falcons mit 10,2 ml Transpo... |
null | null | null | dx.doi.org/10.17504/protocols.io.s2jegcn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is a new protocol for isolation of DNA from from forest soil samples </p>
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.dyv7w5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Mix 7 parts of 100% Ethanol (200-proof) and 3 parts MilliQ water.
[STEPS] | [] |
37,293 | published protocol | null | null | https://www.protocols.io/view/published-protocol-bgnmjvc6 | Val K, Jayesh Kamath, Radhika Kamath | TITLE: published protocol
AUTHORS: Val K, Jayesh Kamath, Radhika Kamath
[STEPS]
?. test
0 µl
?. test
?. ABCD110mlUX234
ABCD110mlUX234 | ["test\n0 µl", "test", "ABCD110mlUX234\nABCD110mlUX234"] |
13,135 | Bead-based normalization for NGS | 1 | null | https://www.protocols.io/view/bead-based-normalization-for-ngs-q3pdymn | Tomasz Suchan | TITLE: Bead-based normalization for NGS
AUTHORS: Tomasz Suchan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Bead-based normalization protocol for genomic libraries based on Hosomichi et al. (2014). It uses diluted AMPure or other SPRI beads solution in order to bind a small amount of DNA from eac... | ["[Preparation]\nPrepare a solution of 20% PEG, 2.5 M NaCl in TE buffer by mixing:5 ml of 5 M NaCl0.83 ml of molecular-grade water100 ul of 1 M Tris20 ul of EDTA50 uL of 10% Tween 104 ml of 50% PEG-8000", "[Preparation]\nDilute AMPure or homemade SeraMag beads 20-fold in the above solution (for instance, add 10 ul of A... |
95,722 | Intracerebroventricular administration of compound in Mus Musculus | 0 | dx.doi.org/10.17504/protocols.io.x54v9pqy1g3e/v1 | https://www.protocols.io/view/intracerebroventricular-administration-of-compound-c9qiz5ue | Jean-Louis Parmasad | TITLE: Intracerebroventricular administration of compound in Mus Musculus
AUTHORS: Jean-Louis Parmasad
[DESCRIPTION]
Intracerebroventricular administration of compound in Mus Musculus. Here we utilize the newly developed CDK14 covalent inhibitor, and inject it into lateral ventricle of Mus Musculus brain.
[STEPS]
SEC... | ["[Day before surgery] 16h before stereotactic surgeries, load Alzet‱ Mini-Osmotic Pumps (model 2004) with 1.47μg/uL of FMF-04-159-2 (R&D Systems 7158, Minneapolis, MN, USA in vehicle solution containing 8% DMSO (Fisher Scientific, BP231, Hampton, NH, USA), 2% Tween 80 (Fisher Scientific, BP338-500) and 90% ddH2O). The... |
83,266 | Immunofluorescence staining for postmortem mouse brain tissue | 1 | dx.doi.org/10.17504/protocols.io.36wgq3reolk5/v1 | https://www.protocols.click/view/immunofluorescence-staining-for-postmortem-mouse-b-cvjaw4ie | Elizabeth P. | TITLE: Immunofluorescence staining for postmortem mouse brain tissue
AUTHORS: Elizabeth P.
[DESCRIPTION]
This is a basic protocol for staining mouse brain tissues using immunofluorescence and immunohistochemistry techniques.
[STEPS]
SECTION: Preparation
1. For this protocol I recommend staining slices in a 12-well p... | ["[Preparation] For this protocol I recommend staining slices in a 12-well plate, and washing the slices in a separate 24-well plate. Each well can hold one solution or wash for the slices (about 1mL of solution in each well should be fine). Be sure to label the plate with the contents of each well.", "[Staining] Wash ... |
66,374 | https://www.facebook.com/viaketoaustralia/ | 3 | dx.doi.org/10.17504/protocols.io.rm7vzydb2lx1/v1 | https://www.protocols.io/view/https-www-facebook-com-viaketoaustralia-cc3esyje | ACV Keto | TITLE: https://www.facebook.com/viaketoaustralia/
AUTHORS: ACV Keto
[DESCRIPTION]
Through Via keto Australia fills in as a hunger suppressant that can diminish the desires for food as well as craving levels
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gqibvue | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>SpinSmart Plasmid Miniprep kits</strong> are designed to rapidly purify plasmid DNA from bacterial cultures.</p>
<p> </p>
<p>The protocol below is appropriate for both <a href="https://www.denvillescientific.com/products/spinsmart%E2%84%A2-plasmid-miniprep-dna-purific... | [] |
59,251 | Standardized Protocol for Transect Set-up and Palm Inventory for the DOPAMICS Research Program | 1 | dx.doi.org/10.17504/protocols.io.14egn73kqv5d/v1 | https://www.protocols.io/view/standardized-protocol-for-transect-set-up-and-palm-b54tq8wn | Julien Engel, Kevin Mabobet, Jean-Louis Smock, Marc Testé, Louise Brousseau | TITLE: Standardized Protocol for Transect Set-up and Palm Inventory for the DOPAMICS Research Program
AUTHORS: Julien Engel, Kevin Mabobet, Jean-Louis Smock, Marc Testé, Louise Brousseau
[DESCRIPTION]
We describe the protocol developed as part of the ERC-funded DOPAMICS research program for setting up transects and co... | ["[Setting-up of the transect central line] Starting from the transect start:\n\nPlant a picket at the start of the transect\nLabel the picket with a permanent marker (Picket ID= \"Cstart\")\nRecord the GPS position of the picket\nFollow the direction of the transect's endpoint using a precision compass and mark the ce... |
86,979 | DIMPLE library generation and assembly protocol | 4 | dx.doi.org/10.17504/protocols.io.rm7vzy7k8lx1/v3 | https://www.protocols.io/view/dimple-library-generation-and-assembly-protocol-cy7bxzin | Christian Macdonald, David Nedrud, Patrick Rockefeller Grimes, Donovan Trinidad, James Fraser, Willow Coyote-Maestas | TITLE: DIMPLE library generation and assembly protocol
AUTHORS: Christian Macdonald, David Nedrud, Patrick Rockefeller Grimes, Donovan Trinidad, James Fraser, Willow Coyote-Maestas
[DESCRIPTION]
This is a protocol for generating and QCing mutagenic libraries using the DIMPLE protocol. This version is updated to inclu... | ["[Preparation] Use DIMPLE to generate mutagenic oligos and primers.", "[Preparation] Important notes: DIMPLE breaks a gene up into sub-library fragments and generates mutagenic insert oligo pools, where each oligo contains barcodes, Type IIS restriction cutsites, and a sub-region of the gene. Be sure to review your li... |
37,377 | Plasmid DNA Mini Kit I- Spin Protocol | null | null | https://www.protocols.io/view/plasmid-dna-mini-kit-i-spin-protocol-bgq9jvz6 | Alex Zegarra | TITLE: Plasmid DNA Mini Kit I- Spin Protocol
AUTHORS: Alex Zegarra
[STEPS]
?. 1) Isolate a single colony from a freshly streaked selective plate, and inoculate a culture of 1- 5 mL LB medium containing the appropriate selective antibiotic. Incubate for ~12-16 hours at 37°C with vigorous shaking (~ 300 rpm). Use a 10... | ["1) Isolate a single colony from a freshly streaked selective plate, and inoculate a culture of 1- 5 mL LB medium containing the appropriate selective antibiotic. Incubate for ~12-16 hours at 37°C with vigorous shaking (~ 300 rpm). Use a 10-20 mL culture tube or a flask with a volume of at least 4 times the volume of ... |
null | null | null | dx.doi.org/10.17504/protocols.io.tvien4e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Aims: Dilated cardiomyopathy (DCM), a myocardial disorder that can result in progressive heart failure and arrhythmias, is defined by ventricular chamber enlargement and dilatation, and systolic dysfunction. Despite extensive research, the pathological mechanisms of DCM are uncl... | ["[Generation of patient-specific induced pluripotent stem cells] Skin biopsies were acquired according to approval #3611 issued by the Helsinki Committee for experiments on human subjects at the Rambam Health Care Campus, Haifa, Israel. The biopsy [human dermal fibroblasts (HDF)] was obtained from a 28-year-old DCM pa... |
54,125 | DNA extraction - Zooplankton - 96 wells | 4 | dx.doi.org/10.17504/protocols.io.j8nlk4x7wg5r/v1 | https://www.protocols.io/view/dna-extraction-zooplankton-96-wells-by4mpyu6 | coline.royaux, Nicolas Rabet, Céline Bonillo | TITLE: DNA extraction - Zooplankton - 96 wells
AUTHORS: coline.royaux, Nicolas Rabet, Céline Bonillo
[DESCRIPTION]
This protocol was used to extract DNA from whole or parts of zooplanktonic freshwater crustaceans (Copepoda, Branchiopoda, ...) from New Caledonia.
[STEPS]
1. Prepare your 96-well extraction plate with o... | ["Prepare your 96-well extraction plate with one individual per well. Alternate genus in the wells to detect eventual contamination between wells.", "Collect one individual from a sample", "Note its genus and determine its sex with a binocular microscope", "For big individuals (more than 5 mm), dissect a few legs and p... |
null | null | null | dx.doi.org/10.17504/protocols.io.rs8d6hw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | ["Rotenone (Sigma R8875) and paraquat were solubilised in DMSO to prepare a 5 mM stock.", "Stock solutions were added to the diet to final treatment concentrations of 0, 3.125, 6.25, 12.5 and 25uM at 50°C", "From the dilution series, we determined an assay concentration of 3.13 µ for both chemicals.", "The dietary rote... |
43,749 | DNT Detection In Soil | 1 | dx.doi.org/10.17504/protocols.io.bnydmfs6 | https://www.protocols.io/view/dnt-detection-in-soil-bnydmfs6 | Zhujun Wei | TITLE: DNT Detection In Soil
AUTHORS: Zhujun Wei
[STEPS]
?. The 1% bacteria (PYB1s-yqjF3rd-luxCDABE) were cultured in LB containing 0.1% streptomycin (50 mg/mL) for 24 h with aeration at 37°C to reach a final density of 1 × 108 cells/mL.
?. Preparation of 2% Alginate solution, 1% calcium-chloride solution, experimenta... | ["The 1% bacteria (PYB1s-yqjF3rd-luxCDABE) were cultured in LB containing 0.1% streptomycin (50 mg/mL) for 24 h with aeration at 37°C to reach a final density of 1 × 108 cells/mL.", "Preparation of 2% Alginate solution, 1% calcium-chloride solution, experimental soil:dissolve 2 g Alginate in 100 mL distilled water (2% ... |
50,706 | Intratibial implantation of tumor cells | 4 | dx.doi.org/10.17504/protocols.io.bvrsn56e | https://www.protocols.io/view/intratibial-implantation-of-tumor-cells-bvrsn56e | Shubhangi Agarwal, donna.peehl , Renuka Sriram | TITLE: Intratibial implantation of tumor cells
AUTHORS: Shubhangi Agarwal, donna.peehl , Renuka Sriram
[DESCRIPTION]
This protocol describes the steps required for the successful implantation of small cell neuroendocrine prostate cancer patient-derived xenograft (PDX) cells in the bone. Bone is one of the most... | ["[Preparation before surgery] Preparation of surgical instruments and supplies: All of the instruments and supplies should be sterilized.", "[Preparation before surgery] Preparation of the PDX cells for implantation", "[Preparation of cells for implantation from frozen biobank] Prepare DMEM medium: Prepare fresh DMEM ... |
57,952 | Borrelia burgdorferi ospC Genotyping Using Luminex Technology | 4 | dx.doi.org/10.17504/protocols.io.b4t8qwrw | https://www.protocols.io/view/borrelia-burgdorferi-ospc-genotyping-using-luminex-b4t8qwrw | Patrick Pearson, Olivia Skaltsis, Chu-Yuan Luo, Guang Xu, Zachary Oppler, Dustin Brisson, Stephen M Rich | TITLE: Borrelia burgdorferi ospC Genotyping Using Luminex Technology
AUTHORS: Patrick Pearson, Olivia Skaltsis, Chu-Yuan Luo, Guang Xu, Zachary Oppler, Dustin Brisson, Stephen M Rich
[DESCRIPTION]
Borrelia burgdorferi is an important tickborne human pathogen and can be grouped into separate strains based on the outer... | ["[PREPARING SOLUTIONS] 1X xTAG buffer\n\nAdd 1 mL of 10X xTAG buffer to 9 mL of molecular grade water. Scale volume up or down as necessary.\nStore at 4°C until use", "[PREPARING SOLUTIONS] Bead mix solution (75 beads/μL)\n\nThe specific Luminex microspheres (beads) are sold in concentrations of 2.5X10^6 beads/mL. The... |
null | null | null | dx.doi.org/10.17504/protocols.io.idmca46 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes a clean up and size selection method for nucleic acids (tested on DNA) to deplete and remove fragments below 1 - 2 kb. <br />The success of this depends on the cleanliness of your sample (it doesn't have to be super clean but a whole lot of contaminant... | [] |
102,634 | Modified nuclease flush protocol for Nanopore RNA flow cells | 0 | null | https://www.protocols.io/view/modified-nuclease-flush-protocol-for-nanopore-rna-dggi3tue | Anthea Hull, Ricardo De Paoli-Iseppi, Mike Clark | TITLE: Modified nuclease flush protocol for Nanopore RNA flow cells
AUTHORS: Anthea Hull, Ricardo De Paoli-Iseppi, Mike Clark
[DESCRIPTION]
A nuclease flush can be used to recover blocked pores on Oxford Nanopore Technologies (ONT) flow cells to maximise overall sequencing output. The Flow Cell Wash Kit (EXP-WSH004/E... | ["[Protocol] Thaw Wash Mix (WMX), RNase H and RNase CocktailTM on ice. Do not vortex.", "[Protocol] Thaw one tube of Wash Diluent (DIL) at room temperature.", "[Protocol] Mix the contents of Wash Diluent (DIL) thoroughly by vortexing, then spin down briefly and place on ice.", "[Protocol] In a fresh 1.5 ml Eppendorf DN... |
96,614 | Phenotypic analysis of root nodules based on paraffin fixation | 0 | dx.doi.org/10.17504/protocols.io.e6nvwdxn2lmk/v1 | https://www.protocols.io/view/phenotypic-analysis-of-root-nodules-based-on-paraf-dake2cte | Carolina Cervera Torres, Kalpana Nanjareddy, Manoj-Kumar Arthikala | TITLE: Phenotypic analysis of root nodules based on paraffin fixation
AUTHORS: Carolina Cervera Torres, Kalpana Nanjareddy, Manoj-Kumar Arthikala
[DESCRIPTION]
The mutualistic relationship between leguminous plants and rhizobia bacteria stands out as one of the most crucial, primarily because of its capacity to conver... | ["[Sample collection] Precision in sample collection is crucial to prevent any damage. Submerge the roots in water to facilitate the handling of nodules. Utilizing tweezers and delicate scissors, carefully sever the roots approximately 1-2 cm away from the nodule. This ensures ease of manipulation during processing wit... |
31,728 | Cardiac Action Potential Protocol | 1 | dx.doi.org/10.17504/protocols.io.ba8qihvw | https://www.protocols.io/view/cardiac-action-potential-protocol-ba8qihvw | Robert Harvey, Shailesh Agarwal | TITLE: Cardiac Action Potential Protocol
AUTHORS: Robert Harvey, Shailesh Agarwal
[DESCRIPTION]
Cardiac Action Potential Protocol
[STEPS]
1. Membrane potentials were recorded from isolated pig ventricular myocytes using the whole-cell configuration of the patch clamp technique.
2. Isolated cells were placed in a per... | ["Membrane potentials were recorded from isolated pig ventricular myocytes using the whole-cell configuration of the patch clamp technique.", "Isolated cells were placed in a perfusion chamber on the stage of an inverted microscope and bathed in an extracellular solution containing (in mM) NaCl 140, KCl 5.4, CaCl22.5, ... |
53,123 | Measuring Coral Skeletal Architecture Using Image Analysis | 1 | dx.doi.org/10.17504/protocols.io.bx5bpq2n | https://www.protocols.io/view/measuring-coral-skeletal-architecture-using-image-bx5bpq2n | Rowan Mclachlan, Ashruti Patel, Andrea Grottoli | TITLE: Measuring Coral Skeletal Architecture Using Image Analysis
AUTHORS: Rowan Mclachlan, Ashruti Patel, Andrea Grottoli
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Coral morphology is influenced by genetics, the environment, or the interaction of both, and thus is highly variable. This ... | ["[Removal of Organic Tissue from Coral Skeletons ]\nRemove coral fragments from the freezer (if applicable) and place them in individual 500 ml plastic beakers filled with deionized water. Soak coral fragments for 30 minutes at room temperature to remove excess salt and prevent NaCl precipitation.", "[Imaging of Coral... |
74,375 | DToL Taxon-specific Standard Operating Procedures for the Plant Working Group | 2 | null | https://www.protocols.io/view/dtol-taxon-specific-standard-operating-procedures-ckvfuw3n | Laura L Forrest, Michelle Hart | TITLE: DToL Taxon-specific Standard Operating Procedures for the Plant Working Group
AUTHORS: Laura L Forrest, Michelle Hart
[DESCRIPTION]
This Standard Operating Procedure (SOP) collection contains guidance on how to process the various plant taxa within the scope of the Darwin Tree of Life (DToL) project. The guida... | [] |
20,452 | Addition of RNA sequins to sample for RNA sequencing. | null | dx.doi.org/10.17504/protocols.io.x8cfrsw | null | Tim Mercer | TITLE: Addition of RNA sequins to sample for RNA sequencing.
AUTHORS: Tim Mercer
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">RNA sequencing can measure both gene or isoform expression, and reconstruct novel and complex spliced isoforms. However, t... | ["[Re-suspension and storage of sequins,]\nUpon receipt of RNA sequins, first check to ensure they have not thawed during shipment., and immediately transfer the RNA sequins to frozen storage at -80°C (sequins should not be stored in a -20°C frost-free freezer).", "[Re-suspension and storage of sequins,]\nEach tube con... |
61,818 | Expression and purification protocol of Homo sapiens E2-like enzyme ATG3 | 4 | dx.doi.org/10.17504/protocols.io.36wgq7mnkvk5/v1 | https://www.protocols.io/view/expression-and-purification-protocol-of-homo-sapie-b8k2ruye | Liv Jensen, Eleonora Turco, Dorotea Fracchiolla | TITLE: Expression and purification protocol of Homo sapiens E2-like enzyme ATG3
AUTHORS: Liv Jensen, Eleonora Turco, Dorotea Fracchiolla
[DESCRIPTION]
This protocol describes expression and purification procedures for obtaining human recombinant autophagy E2-like enzyme ATG3 (ATG, AuTophaGy-related protein) of the AT... | ["[Protein Purification] Cells are lised via sonication: thaw pellet corresponding to 1 L culture. All following steps are to be executed at 4 °C or on ice.", "[Protein Purification] Lyse cells by sonicating them using an immersion tip Sonicator (2x 30 s). Note: adjust times and intensity according to the available ins... |
82,430 | RNA isolation and qRT-PCR | 4 | dx.doi.org/10.17504/protocols.io.261ge3qojl47/v1 | https://www.protocols.click/view/rna-isolation-and-qrt-pcr-cuq6wvze | Narayana Yadavalli, Shawn M. Ferguson | TITLE: RNA isolation and qRT-PCR
AUTHORS: Narayana Yadavalli, Shawn M. Ferguson
[DESCRIPTION]
This protocol describes the RNA isolation from cultured cells and quantitative RT PCR.
[STEPS]
SECTION: RNA isolation and qRT-PCR
1. Aspirate media from cells and rinse cells with PBS on ice.
SECTION: RNA isolation and qRT-... | ["[RNA isolation and qRT-PCR] Aspirate media from cells and rinse cells with PBS on ice.", "[RNA isolation and qRT-PCR] Isolate RNA using RNeasy Micro Plus kit (Qiagen) according to manufacturer’s protocol.", "[RNA isolation and qRT-PCR] Generate cDNA from 1 µg purified RNA using iScript cDNA synthesis Kit (Bio-Rad) ac... |
106,733 | IMARIS Analyses of LAMP_CATD in vitro | 0 | dx.doi.org/10.17504/protocols.io.81wgbzozygpk/v1 | https://www.protocols.io/view/imaris-analyses-of-lamp-catd-in-vitro-dkgm4tu6 | Chiara Pavan, Stefano Frausin, Clare Parish, Lachlan Thompson | TITLE: IMARIS Analyses of LAMP_CATD in vitro
AUTHORS: Chiara Pavan, Stefano Frausin, Clare Parish, Lachlan Thompson
[DESCRIPTION]
This protocol details how to analyse in-vitro LAMP and cathepsin D staining using Imaris software.
[BEFORE_START]
Select your image files folders. Drag the .LIF files into the Imaris Arena... | ["[Creation of MAP2 surface] Add new surface.\nLeave Creation Parameters as default\nSelected segment only ROI.\nClick next. Drag the yellow box to select the cell. Whole cell of interest.\nClick Next. Select the channel (for your marker of interest). Smooth Surface detail. 0.0360. Machine learning segmentation.\nClick... |
98,234 | Electrophysiological recording from Brain Slices Protocol | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8o7olmk/v1 | https://www.protocols.io/view/electrophysiological-recording-from-brain-slices-p-db622rge | HaiDun | TITLE: Electrophysiological recording from Brain Slices Protocol
AUTHORS: HaiDun
[DESCRIPTION]
This protocol details the steps for performing slice electrophysiological recordings with DART.
[STEPS]
SECTION: Brain slice preparation
1. Take 150 mL-200 mLhigh sucrose solution cutting solution, store at-80 °C for 15 min... | ["[Brain slice preparation] Take 150 mL-200 mLhigh sucrose solution cutting solution, store at-80 °C for 15 min-20 min to make chilled half ice solution.", "[Brain slice preparation] Deeply anesthetize the mouse with isoflurane, and then decapitate.", "[Brain slice preparation] Take brain and place the brain into pre-c... |
83,164 | Genotyping mice from ear clips | 4 | dx.doi.org/10.17504/protocols.io.5qpvo3xobv4o/v1 | https://www.protocols.click/view/genotyping-mice-from-ear-clips-cvf4w3qw | Elizabeth P. | TITLE: Genotyping mice from ear clips
AUTHORS: Elizabeth P.
[DESCRIPTION]
This is a protocol that describes the genotyping procedure from ear clip samples. This includes a general PCR protocol for primers with annealing temperatures in the range 55-70 degrees C. For other primers, the thermal cycling should be adjuste... | ["[Tissue Collection] Scruffing mouse securely, snip a tissue sample approximately 4mm2 in size from the edge of its ear using a punch or scissors.", "[Tissue Collection] Store and transport samples separately in clearly-labelled 1.5mL tubes.", "[DNA Extraction] Add 200uL of 20mM NaOH to each tissue sample.", "[DNA Ext... |
20,062 | U Mass - Cholesterol (HDL) | null | dx.doi.org/10.17504/protocols.io.xt6fnre | null | Jason Kim | TITLE: U Mass - Cholesterol (HDL)
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels ... | ["Perform daily quality control assessment of instrumentation before analysis.", "Load each sample into a specialized micro-sample cup for the clinical chemistry analyzer.", "Select Cholesterol (HDL) test on display and run the analysis.", "Collect and analyze the data."] |
7,386 | Protocol for multiplex (6-plex) measurement of anti-malarial antibodies | 1 | dx.doi.org/10.17504/protocols.io.jf2cjqe | https://www.protocols.io/view/protocol-for-multiplex-6-plex-measurement-of-anti-jf2cjqe | Kwadwo Kusi | TITLE: Protocol for multiplex (6-plex) measurement of anti-malarial antibodies
AUTHORS: Kwadwo Kusi
[DESCRIPTION]
This protocol provides a step-by-step description of the process for attaching proteins to carboxylated microspheres and the optimization processes for mixing different antigen-coupled microspheres and se... | [] |
42,186 | Protocols for PCR | 2 | null | https://www.protocols.io/view/protocols-for-pcr-bmfik3ke | TITLE: Protocols for PCR
AUTHORS:
[STEPS] | [] | |
null | null | null | dx.doi.org/10.17504/protocols.io.nbxdapn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Simple and rapid vacuole staining protocol for Phaeodactylum tricornutum. Note that RatioWorks<sup>TM</sup> PDMPO and LysoSensor<sup>TM</sup> Yellow/Blue DND-160 are the same thing and can thus be used interchangeably. RatioWorks<sup>TM</sup> PDMPO was used in this protocol.... | [] |
45,133 | Ancient DNA Extraction from Dental Calculus with Consolidant Removal | 1 | dx.doi.org/10.17504/protocols.io.bqbmmsk6 | https://www.protocols.io/view/ancient-dna-extraction-from-dental-calculus-with-c-bqbmmsk6 | Franziska Aron, Courtney Hofman, Zandra Fagernäs, Irina Velsko, Guido Brandt, Christina Warinner | TITLE: Ancient DNA Extraction from Dental Calculus with Consolidant Removal
AUTHORS: Franziska Aron, Courtney Hofman, Zandra Fagernäs, Irina Velsko, Guido Brandt, Christina Warinner
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Silica-based total DNA extraction protocol optimised for the rec... | ["[Day 1: Preparation of reagents (Buffer Prep Room)]\nPrepare cleaned workspace with all necessary reagents and equipment.\nIf lab-wide large-batch pre-prepared reagent stores are used, ensure to have made personal stock-aliquot of reagents such as UV-Water, EDTA, sodium acetate, and proteinase K in amounts sufficient... |
54,937 | Test protocol II | 1 | null | https://www.protocols.io/view/test-protocol-ii-bzvzp676 | Abby Moore | TITLE: Test protocol II
AUTHORS: Abby Moore
[DESCRIPTION]
The purpose of this protocol is to demo the NMRFAM template in protocols.io
The scope of this protocol applies to the NAN Knowledgebase groups.
Here's a protocol:
[BEFORE_START]
Type pertinent info about what the user should know before starting the pr... | ["Use 80:20 MeOH:H2O for this step. This is not easy to access by machine.", "If you haven't already, make a solution with the following components:\n\n80 % volume \n20 % volume", "If you haven't already, make a solution with the following components:\n\n80 % volume \n20 % volume", "Use this piece of equipment:"] |
21,977 | Toxoplasmosis and mental disorders in the Russian Federation | null | dx.doi.org/10.17504/protocols.io.zpzf5p6 | null | Ekaterina V. Stepanova, Anatoly V. Kondrashin, Vladimir P. Sergiev, Lola F. Morozova, Natalia A. Turbabina, Maria S. Maksimova, Dmitry V. Romanov, Marina A. Kinkulkina, Alena V. Lazareva (Lukianova), Evgeny Morozov | TITLE: Toxoplasmosis and mental disorders in the Russian Federation
AUTHORS: Ekaterina V. Stepanova, Anatoly V. Kondrashin, Vladimir P. Sergiev, Lola F. Morozova, Natalia A. Turbabina, Maria S. Maksimova, Dmitry V. Romanov, Marina A. Kinkulkina, Alena V. Lazareva (Lukianova), Evgeny Morozov
[DESCRIPTION]
<div class = ... | [] |
97,110 | USDA LTAR Common Experiment measurement: Sediment flux | 1 | dx.doi.org/10.17504/protocols.io.6qpvr8x23lmk/v1 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-sediment-f-da3w2gpe | Brandon L. Edwards, Nicholas P. Webb, Justin W. Van Zee, Ericha M. Courtright | TITLE: USDA LTAR Common Experiment measurement: Sediment flux
AUTHORS: Brandon L. Edwards, Nicholas P. Webb, Justin W. Van Zee, Ericha M. Courtright
[DESCRIPTION]
Wind erosion from agroecosystems can harm ecosystem functions, environmental quality, and human health. Wind erosion can also lead to reduced economic retur... | ["[Soil characteristics] Identify a representative plot area measuring 100m x 100m in size.", "[Soil characteristics] Partition the site into nine equivalently sized areas using a 3 × 3 grid.", "[Soil characteristics] Randomly assign three sampling locations within each grid cell to Sample Groups 1, 2, and 3 (Fig. 1)."... |
92,422 | Splitting Adherent Cell Lines | 4 | dx.doi.org/10.17504/protocols.io.x54v9p51qg3e/v1 | https://www.protocols.io/view/splitting-adherent-cell-lines-c6hezb3e | Carolina Lopez | TITLE: Splitting Adherent Cell Lines
AUTHORS: Carolina Lopez
[DESCRIPTION]
This protocol describes how to split and maintain adherent cell lines in culture. Examples of these cells are: A549 cells, LLCMK2 cells, and MDCK cells.
[STEPS]
SECTION: Protocol
1. Splitting Adherent cells
Wash flask 2x with sterile PBS.
Add ... | ["[Protocol] Splitting Adherent cells\nWash flask 2x with sterile PBS.\nAdd 2mL of trypsin/T75. Incubate at 37 oC for 2-3 mins or until cells are detached from the flask.\nAdd 8mL of cell culture media and pipette up and down. Transfer all the media to a 15mL tube. \nCentrifuge at 1200 rpm for 5 min. \nAdd 2ml of cell ... |
51,235 | Processamento de actígrafos pré-coleta - ActTrust - V.1 | 5 | dx.doi.org/10.17504/protocols.io.bwabpaan | https://www.protocols.io/view/processamento-de-act-grafos-pr-coleta-acttrust-v-1-bwabpaan | Daniel Vartanian | TITLE: Processamento de actígrafos pré-coleta - ActTrust - V.1
AUTHORS: Daniel Vartanian
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Este protocolo faz parte do processo de coleta de dados actigráficos do Grupo Interdisciplinar de Pesquisa em Sono (GIPSO). Ele foi desenhado para os actígra... | ["[Kanban]\nAdicione o cartão da tarefa no quadro de trabalho do seu projeto (somente para projetos que usam Kanban)\nAbra o quadro de trabalho de seu projeto e adicione um cartão chamado Realizar o processamento de actígrafos pré-coleta na lista Em andamento. Ao clicar sobre o cartão criado, vá em Etiquetas e selecion... |
47,742 | Protein-A_Protein-LA Sandwich ELISA | 1 | dx.doi.org/10.17504/protocols.io.bsu6neze | https://www.protocols.io/view/protein-a-protein-la-sandwich-elisa-bsu6neze | Angel Justiz-Vaillant | TITLE: Protein-A_Protein-LA Sandwich ELISA
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) and protein-LA (SpLA)with different immunoglobulin preparations of mammalian and avian speci... | ["The plate was then treated with bovine serum albumin solution and washed 4X with PBS-Tween. 50 µl of immunoglobulins (1 mg/ml) is added and incubated for 1h at room temperature, and the microplate is rewashed 4X with PBS-Tween.", "This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) an... |
60,501 | NCBI submission protocol for foodborne virus surveillance | 1 | dx.doi.org/10.17504/protocols.io.j8nlkkdbxl5r/v1 | https://www.protocols.io/view/ncbi-submission-protocol-for-foodborne-virus-surve-b7bvrin6 | Zhihui Yang, Ruth Timme, Maria Balkey, Zhihui Yang | TITLE: NCBI submission protocol for foodborne virus surveillance
AUTHORS: Zhihui Yang, Ruth Timme, Maria Balkey, Zhihui Yang
[DESCRIPTION]
INTRODUCTION:
This protocol outlines the steps which ViroTrakr contributors need to follow in order to submit their data to NCBI. It includes how to:
- establish your new Bio... | ["[ViroTrakr data structure] ViroTrakr database structure: the ViroTrakr database was established as an umbrella BioProject at NCBI with the structure shown below:\n \n \nDatabase structure: (cont.) for each data level BioProject:", "[NCBI sign in] Getting started\n\nPlease refer to “NCBI submission protocol for micro... |
54,153 | RCA of Gotcha | 4 | dx.doi.org/10.17504/protocols.io.by5hpy36 | https://www.protocols.io/view/rca-of-gotcha-by5hpy36 | Chia-Hsien Shih | TITLE: RCA of Gotcha
AUTHORS: Chia-Hsien Shih
[DESCRIPTION]
This protocol is to test that GotCha is functional and can works as designed.
[STEPS]
SECTION: Preparation
1. Add 5 µL of GotCha(functional beads) into eppendorf
SECTION: Preparation
2. Centrifuge for 15000 rpm, 5 min and remove supernatant. Make sure that... | ["[Preparation] Add 5 µL of GotCha(functional beads) into eppendorf", "[Preparation] Centrifuge for 15000 rpm, 5 min and remove supernatant. Make sure that eppendorf should put on DynaMag when removing supernatant.", "[Protocol] Add 3 µL of 10X phi29 polymerase reaction buffer into eppendorf with GotCha", "[Protocol] A... |
20,771 | UC Davis - ELISA Protocol | null | dx.doi.org/10.17504/protocols.io.yibfuan | null | Peter Havel | TITLE: UC Davis - ELISA Protocol
AUTHORS: Peter Havel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">The ELISA (Enzyme Linked ImmunoSorbent Assay) is an assay method used for the quantification of various proteins. A ... | ["Follow kit instructions for volumes and incubation times. Pipet standards and samples. Incubate.", "Follow kit instructions for volumes and incubation times. Wash plate. Add secondary antibody. Incubate.", "Follow kit instructions for volumes and incubation times. Wash plate. Add substrate. Incubate.", "Follow kit in... |
97,028 | Cross-modality frame alignment for surgical robot electrode insertion | 0 | dx.doi.org/10.17504/protocols.io.5jyl82rb7l2w/v1 | https://www.protocols.io/view/cross-modality-frame-alignment-for-surgical-robot-dazc2f2w | Lucy Liang, Elvira Pirondini, Jonathan C Ho | TITLE: Cross-modality frame alignment for surgical robot electrode insertion
AUTHORS: Lucy Liang, Elvira Pirondini, Jonathan C Ho
[DESCRIPTION]
Scanners for animals often use different image orientations as in clinical scanners. Multi-modal imaging further introduces shifts between images since the animal cannot be pl... | ["Open the ROSA ONE Brain software, either on a ROSA computer or the ROSA ONE robot.", "Select add new subject, stereotaxy, import 3D images from USB.\n \n\n \n\n \n* There is a limit for image resolution in this software, so your images may be downsampled.", "Once both MRI and CT are added, the program will prompt for... |
91,194 | Coating of tissue Culture Vessels for hPSC | 4 | dx.doi.org/10.17504/protocols.io.x54v9pz24g3e/v1 | https://www.protocols.io/view/coating-of-tissue-culture-vessels-for-hpsc-c5a2y2ge | Lisa Pavinato, Valeria Fernandez Vallone, Lyn Healy, Nathalie Lefort, Katarzyna Ludwik, Tamer Onder, Fatma Visal FVO OKUR, Harald Stachelscheid | TITLE: Coating of tissue Culture Vessels for hPSC
AUTHORS: Lisa Pavinato, Valeria Fernandez Vallone, Lyn Healy, Nathalie Lefort, Katarzyna Ludwik, Tamer Onder, Fatma Visal FVO OKUR, Harald Stachelscheid
[DESCRIPTION]
This protocol describes procedures to coat culture vessels for the maintenance of hPSC using differen... | ["[Aliquoting and storage of Vitronectin] Upon receipt, aliquot Vitronectin and store vials according to manufacturer instructions (Table 2).\n\n\n \n\nDo not store aliquots in frost-free freezer. Do not repeatedly freeze and thaw.", "[Coating] Thaw a vial of Vitronectin at Room temperature for 2 min.", "[Matrix select... |
100,084 | Twist2 Fresh Frozen | 0 | null | https://www.protocols.io/view/twist2-fresh-frozen-ddyu27ww | Griffen Wakelin | TITLE: Twist2 Fresh Frozen
AUTHORS: Griffen Wakelin
[DESCRIPTION]
Immunofluorescence protocol for Twist2 staining of fresh frozen mouse tissue.
[STEPS]
SECTION: Stain
1. Thaw slides, dry for 30 mins. Pap and label.
SECTION: Stain
2. Post-fix with ice-cold PFA 4 % (v/v) 5 min
SECTION: Stain
3. Wash with 1X PBS 5 min ... | ["[Stain] Thaw slides, dry for 30 mins. Pap and label.", "[Stain] Post-fix with ice-cold PFA 4 % (v/v) 5 min", "[Stain] Wash with 1X PBS 5 min", "[Stain] Wash with 1X PBS 5 min", "[Stain] Wash with 1X PBS 5 min", "[Stain] Permeabilize with Triton X-100 0.2 % (v/v) 10 min", "[Stain] Wash with 1X PBS 5 min", "[Stain] Was... |
54,386 | Protein purification | 1 | null | https://www.protocols.io/view/protein-purification-bzcsp2we | Yuichiroh Ikagawa | TITLE: Protein purification
AUTHORS: Yuichiroh Ikagawa
[DESCRIPTION]
The extraction method is based on the paper by Janice S. Chen et al. The cultured E. coli is collected by centrifugation and then disrupted by sonication. The extracted solution is purified by affinity chromatography using a Ni-NTA column with an x1... | ["[protein purification] Fully suspend the matrix before opening the column.4 °C", "[protein purification] Attach the bottom stopper.", "[protein purification] Add cell extraction sample to the column.4 °C", "[protein purification] Install the column in a vertical position and let the resin settle at the bottom of the ... |
86,450 | CAMbank: cfDNA BCT Field Processing v1 | 4 | null | https://www.protocols.io/view/cambank-cfdna-bct-field-processing-v1-cynsxvee | Eliah G Overbey, Krista A Ryon, jak, chm2042 | TITLE: CAMbank: cfDNA BCT Field Processing v1
AUTHORS: Eliah G Overbey, Krista A Ryon, jak, chm2042
[DESCRIPTION]
Field processing of cfDNA BCTs for the Cornell Aerospace Medicine Biobank (CAMbank).
Instructions for preserving: plasma and RBC Pellets.
[STEPS]
SECTION: Perform Venipuncture
1. After venipuncture, inve... | ["[Perform Venipuncture] After venipuncture, invert the tubes gently 8 to 10 times to fully mix tube anticoagulant with blood sample.\n\nStore the tube upright at room temperature until centrifugation.", "[Centrifuge Settings: Plasma Separation] Note: A swing bucket centrifuge is required. \n\nSet centrifuge:\naccelera... |
45,296 | Genotyping of the rs1695 polymorphism of the GSTP1 gene by PCR-RFLP | 1 | dx.doi.org/10.17504/protocols.io.bqgqmtvw | https://www.protocols.io/view/genotyping-of-the-rs1695-polymorphism-of-the-gstp1-bqgqmtvw | Jéssica Barletto de Sousa Barros, Kamilla de Faria Santos, Rômulo Morais Azevedo, Rayana Pereira Dantas de Oliveira, Ana Carolina Dourado Leobas, Dhiogo da Cruz Pereira Bento, Rodrigo da Silva Santos, Angela Adamski da Silva Reis | TITLE: Genotyping of the rs1695 polymorphism of the GSTP1 gene by PCR-RFLP
AUTHORS: Jéssica Barletto de Sousa Barros, Kamilla de Faria Santos, Rômulo Morais Azevedo, Rayana Pereira Dantas de Oliveira, Ana Carolina Dourado Leobas, Dhiogo da Cruz Pereira Bento, Rodrigo da Silva Santos, Angela Adamski da Silva Reis
[DESC... | ["[DNA extraction]\nDNA samples were isolated from peripheral blood using DNA extraction kit (Invitrogen®), according to the manufacturer's instructions. GSTP1 rs1695 variant was genotyped through PCR-RFLP method.", "[GSTP1 gene amplification by PCR]\nA conventional polymerase chain reaction (PCR) was performed to ampl... |
33,231 | Environmental DNA (eDNA) 12S Metabarcoding Illumina MiSeq NGS PCR Protocol (Touchdown) | null | dx.doi.org/10.17504/protocols.io.bcppivmn | null | Katie Pitz, Nathan Truelove, Charles Nye, Reiko P Michisaki, Francisco Chavez | TITLE: Environmental DNA (eDNA) 12S Metabarcoding Illumina MiSeq NGS PCR Protocol (Touchdown)
AUTHORS: Katie Pitz, Nathan Truelove, Charles Nye, Reiko P Michisaki, Francisco Chavez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The 12S protocol is aimed at amplifying the hypervariable region of the... | ["[Setup and Primary PCR]\neDNA template & PCR processing were performed at the Monterey Bay Aquarium Research Institute (MBARI).PCR reactions for the 12S locus were performed with a two-step amplification protocol for each sample using the MiFish_U primers (Miya et al. 2015) with Fluidigm adapters CS1 & CS2.All primer... |
45,551 | 1.1.1. Membrane protein separation | 4 | null | https://www.protocols.io/view/1-1-1-membrane-protein-separation-bqqpmvvn | Elizabeth Fozo | TITLE: 1.1.1. Membrane protein separation
AUTHORS: Elizabeth Fozo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Separation of membrane and cytoplasmic protein</div></div>
[STEPS]
?. [Separation of membrane and cytoplasmic protein]
Using micro-centrifuge in the labSpin 17,000 g for 30 minutesTrans... | ["[Separation of membrane and cytoplasmic protein]\nUsing micro-centrifuge in the labSpin 17,000 g for 30 minutesTransfer supernatant (Cytoplasmic fraction), wash the pellet with cold PBS and resuspend in 100ul of PBS (membrane fraction)", "[Separation of membrane and cytoplasmic protein]\nUse Ultracentrifuge (100,000 ... |
83,324 | Purification of ACOD1 expressed in E. coli | 4 | dx.doi.org/10.17504/protocols.io.14egn2npyg5d/v2 | https://www.protocols.click/view/purification-of-acod1-expressed-in-e-coli-cvk4w4yw | Konrad Büssow | TITLE: Purification of ACOD1 expressed in E. coli
AUTHORS: Konrad Büssow
[DESCRIPTION]
This protocol describes the purification of the human enzyme cis-aconitate decarboxylase (ACOD1) from recombinant E. coli cells.
[BEFORE_START]
The expression plasmid pCAD29 (pCAD29_hIRG1_4-461_pvp008, Addgene #124843) is used. It... | ["[Protein expression] Inoculate 20 ml MDAG-135 containing 100 mg/L kanamycin and chloramphenicol with a colony and shake over night at 37°C.", "[Protein expression] Prepare 4×1 L ZYM-5052 medium with 100 mg/L kanamycin and 34 mg/L chloramphenicol in 2.8 L baffled Fernbach flasks. Inoculate each Fernbach flask with 4 m... |
95,381 | Quantification of SARS-CoV-2 in wastewater | 1 | dx.doi.org/10.17504/protocols.io.81wgbx39ylpk/v1 | https://www.protocols.io/view/quantification-of-sars-cov-2-in-wastewater-c9dvz266 | David Ian Walker, James Lowther, Nick Evens, Jonathan Warren, Jonathan Porter, Kata Farkas, Davey Jones | TITLE: Quantification of SARS-CoV-2 in wastewater
AUTHORS: David Ian Walker, James Lowther, Nick Evens, Jonathan Warren, Jonathan Porter, Kata Farkas, Davey Jones
[DESCRIPTION]
This procedure describes the concentration and quantification of SARS-CoV-2 from wastewater as used by the Environmental Monitoring for Health... | ["[Preparation of daily phi6 process control working suspensions] On each day of testing, a new batch of phi6 process control working suspension should be made.", "[Preparation of daily phi6 process control working suspensions] Remove an aliquot of phi6 stock from the freezer (prepared according to Appendix 1), thaw an... |
null | null | null | dx.doi.org/10.17504/protocols.io.ebebaje | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is from:<br /><br />Hare EE, Peterson BK, Iyer VN, Meier R, Eisen MB (2008) Sepsid <em>even-skipped</em> Enhancers Are Functionally Conserved in <em>Drosophila</em> Despite Lack of Sequence Conservation. PLoS Genet 4(6): e1000106. doi:10.1371/journal.pgen.1000106<b... | [] |
28,661 | A fast and simple fluorometric method to detect cell death in 3D intestinal organoids | null | dx.doi.org/10.17504/protocols.io.78vhrw6 | null | Konstantin J. Bode, Stefanie Mueller, Matthias Schweinlin, Marco Metzger, Thomas Brunner | TITLE: A fast and simple fluorometric method to detect cell death in 3D intestinal organoids
AUTHORS: Konstantin J. Bode, Stefanie Mueller, Matthias Schweinlin, Marco Metzger, Thomas Brunner
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes a novel fluorometric method for the q... | ["[Preparing organoids for staining]\nGrow murine intestinal organoids as described, preferably in clear 96-well plates.", "[Staining of organoids with Propidium Iodide & Hoechst 33342]\nCheck effects of treatment — morphological differences in treated vs. untreated organoids.", "[Staining of organoids with Propidium I... |
20,375 | Applying vContact to Viral Sequences and Visualizing the Output (Cyverse) | null | dx.doi.org/10.17504/protocols.io.x5xfq7n | null | Benjamin Bolduc | TITLE: Applying vContact to Viral Sequences and Visualizing the Output (Cyverse)
AUTHORS: Benjamin Bolduc
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>A collection of protocols designed to guide the user in processing a viral metagenome from raw sequence data to assembly, and subsequent ana... | ["[Affiliating contigs through their shared proteins]\nOpen vConTACT2 Open vContact2-0.9.5 from 'Apps'\nvConTACT2 is constantly in a state of active development. Always check back here for newer protocols that often simply update the version. And always use the most recent version whenever possible.", "[Affiliating con... |
83,982 | Characterizing spatial and temporal properties of ER-phagy receptors | 4 | null | https://www.protocols.click/view/characterizing-spatial-and-temporal-properties-of-cv9nw95e | Melissa Hoyer, Harper JW | TITLE: Characterizing spatial and temporal properties of ER-phagy receptors
AUTHORS: Melissa Hoyer, Harper JW
[DESCRIPTION]
The endoplasmic reticulum (ER) has a vast proteomic landscape to preform many diverse functions including protein and lipid synthesis, calcium ion flux, and inter-organelle communication. The ER ... | ["[Generation of Stable Cell ES H9 Ngn2 line expressing ER receptors] Electroporation of PB vectors. Use ThermoFisher kit and ThermoFisher Neon Electroporator to electroporate ES cells with PB vector and PB helper vector.", "[Generation of Stable Cell ES H9 Ngn2 line expressing ER receptors] Add 10microliter buffer R t... |
52,478 | Preferential Lysis of S. rosetta for Total RNA (VERSION 2) | 4 | dx.doi.org/10.17504/protocols.io.261ge4bojv47/v1 | https://www.protocols.io/view/preferential-lysis-of-s-rosetta-for-total-rna-vers-bxg6pjze | David Booth | TITLE: Preferential Lysis of S. rosetta for Total RNA (VERSION 2)
AUTHORS: David Booth
[DESCRIPTION]
Sterol-based detergents, like digitonin more effectively disrupt membranes with sterols, like those of eukaryotes. This protocol leverages the different membrane compositions of eukaryotes and bacteria to preferentiall... | ["[Prepare Lysis Buffer] Combine the following components for the lysis buffer:", "[Count Cells] Determine the total number of cells in the culture that will be harvested using a hemocytometer.", "[Count Cells] Fix 200 µl of cells with 2 µl of 37% formaldeyde and vortex well.", "[Count Cells] Pipet up and down to homog... |
81,470 | Organelle isolation from mouse tissues expressing organelle tags | 4 | dx.doi.org/10.17504/protocols.io.x54v9d61zg3e/v1 | https://www.protocols.io/view/organelle-isolation-from-mouse-tissues-expressing-cts6wnhe | Rotimi Fasimoye, Emily Dickie, Matthew Taylor, Francesca Tonelli, Dario R Alessi | TITLE: Organelle isolation from mouse tissues expressing organelle tags
AUTHORS: Rotimi Fasimoye, Emily Dickie, Matthew Taylor, Francesca Tonelli, Dario R Alessi
[DESCRIPTION]
We describe here a method to perform the rapid isolation of intact lysosomes from mouse tissues expressing a lysosome-localized TMEM192-3×HA fu... | ["[1) Anti-HA Magnetic beads preparation] 1.1) Transfer n x 100 µl of anti-HA Magnetic Beads (where n = number of samples) into a low binding Eppendorf tube on ice.", "[1) Anti-HA Magnetic beads preparation] 1.2) Immobilize the beads by placing the tube into a Dyna-Mag tube holder for 30s.", "[1) Anti-HA Magnetic beads... |
90,732 | Tissue culture of HeLa cells | 4 | dx.doi.org/10.17504/protocols.io.kxygx31oog8j/v1 | https://www.protocols.io/view/tissue-culture-of-hela-cells-c4ukywuw | Dan Tudorica | TITLE: Tissue culture of HeLa cells
AUTHORS: Dan Tudorica
[DESCRIPTION]
Growth in DMEM supplemented with FBS and antibiotics
[STEPS]
1. Medium: To maintain HeLa cells, grow in Dulbecco's Modified Eagle Medium supplemented with 10% FBS and 1x Pen-strep. Grow in a sterile incubator at 37 C and 5% CO2. Cells double roug... | ["Medium: To maintain HeLa cells, grow in Dulbecco's Modified Eagle Medium supplemented with 10% FBS and 1x Pen-strep. Grow in a sterile incubator at 37 C and 5% CO2. Cells double roughly every 24 H. Grow on standard tissue culture-treated plasticware", "Splitting instructions: Split 1:4 on Mondays and Wednesday, and 1... |
55,237 | Utilizing the Public GenomeTrakr Database for Foodborne Pathogen Traceback | 2 | dx.doi.org/10.17504/protocols.io.bz7dp9i6 | https://www.protocols.io/view/utilizing-the-public-genometrakr-database-for-food-bz7dp9i6 | Ruth Timme, Maria Sanchez, Marc Allard | TITLE: Utilizing the Public GenomeTrakr Database for Foodborne Pathogen Traceback
AUTHORS: Ruth Timme, Maria Sanchez, Marc Allard
[DESCRIPTION]
This protocol outlines the all the steps necessary to become a GenomeTrakr data contributor. GenomeTrakr is an international genomic reference database of mostly food and e... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.fv2bn8e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><em>The EpiQuik™ Total Histone Extraction Kit</em> is a complete set of optimized buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissues in a simple 60 minute procedure. The post-translational modifications (PTM)... | [] |
22,608 | Robust measurement of the real world effectiveness of Tofacitinib for the treatment of Ulcerative Colitis using electronic health records: a protocol and statistical analysis plan | null | dx.doi.org/10.17504/protocols.io.2bqgamw | null | Vivek A. Rudrapatna, Atul J. Butte | TITLE: Robust measurement of the real world effectiveness of Tofacitinib for the treatment of Ulcerative Colitis using electronic health records: a protocol and statistical analysis plan
AUTHORS: Vivek A. Rudrapatna, Atul J. Butte
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The "efficacy-effecti... | ["[Validating prospective MRNs using the 'Received Tofacitinib' spreadsheet]\nDiagnosis:{UC, CD, NA}The best place to unambiguously identify a working diagnosis is a recent colonoscopy done near the time of treatment initiation. In particular, the indication, impression, and recommendations section are often quite usef... |
16,158 | Microstructures morphology - Ophiuroidea | null | dx.doi.org/10.17504/protocols.io.tz6ep9e | null | Renata Alitto, Michela Borges, Helena Serrano, Letícia de Oliveira Dias | TITLE: Microstructures morphology - Ophiuroidea
AUTHORS: Renata Alitto, Michela Borges, Helena Serrano, Letícia de Oliveira Dias
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Study of the microstructure morphology, particularly arm ossicles of ophiuroids. </div></div>
[STEPS]
?. [Choose]
Choose t... | ["[Choose]\nChoose the best-preserved specimens.", "[Extract a piece of arm]\nExtract from a small part of the proximal arm, between the fifth and the tenth segment.", "[Household bleach]\nImmerse the arm segment in regular household bleach (NaClO) until the soft tissues be removed.", "[Prepare the stub to observe on S... |
80,691 | Patient Information Sheet 1.2 | 3 | dx.doi.org/10.17504/protocols.io.4r3l27k84g1y/v1 | https://www.protocols.click/view/patient-information-sheet-1-2-cs2twgen | Christopher Hawthorne, Keenan Smith, Malcolm Watson, Martin Shaw, Jonathan Cavanagh, Eric Jackson, Shona McKay, Matthew Sheridan | TITLE: Patient Information Sheet 1.2
AUTHORS: Christopher Hawthorne, Keenan Smith, Malcolm Watson, Martin Shaw, Jonathan Cavanagh, Eric Jackson, Shona McKay, Matthew Sheridan
[DESCRIPTION]
Patient information sheet for Predicting Cognitive Decline After Spinal Surgery (PROTECT).
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.r6yd9fw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | ["Collect eggs from adults of both mitotypes raised on instant food", "Posit eggs onto experimental diet", "Collect eclosing females as virgins", "Place virgins in vials containing experimental diet, with two females per vial", "Add one male of each mitotype to vials containing virgin females", "After 24 hours move fli... |
63,430 | Steve Harvey CBD Gummies – (2022) Top Best CBD Gummies to UseProducts for Sale CBD Gummy Brand Comparison ListIS IT SCAM OR LEGIT? | 1 | dx.doi.org/10.17504/protocols.io.261genjoyg47/v1 | https://www.protocols.io/view/steve-harvey-cbd-gummies-2022-top-best-cbd-gummies-b97er9je | asns | TITLE: Steve Harvey CBD Gummies – (2022) Top Best CBD Gummies to UseProducts for Sale CBD Gummy Brand Comparison ListIS IT SCAM OR LEGIT?
AUTHORS: asns
[DESCRIPTION]
Steve Harvey CBD Gummies
[STEPS]
1. Steve Harvey CBD Gummies
➢Product Name —Steve Harvey CBD Gummies Reviews
➢Main Benefits—Improve Metabolism & H... | ["Steve Harvey CBD Gummies\n\n \n\n➢Product Name —Steve Harvey CBD Gummies Reviews\n➢Main Benefits—Improve Metabolism & Help in Pain Relief\n➢Composition —NaturalOrganic Compound\n➢Side-Effects—NA\n➢Rating :—⭐⭐⭐⭐⭐\n➢Availability —Online\n➢Price (for Sale) Buy Now Here —Click Here\nSteve Harvey CBD Gummies - You plan to... |
22,027 | A Review of Hypotoxic Fluorescent Nanoparticles Delivery by Cell-Penetrating Peptides in Multiple Organisms | null | dx.doi.org/10.17504/protocols.io.zrjf54n | null | Cathy Miller | TITLE: A Review of Hypotoxic Fluorescent Nanoparticles Delivery by Cell-Penetrating Peptides in Multiple Organisms
AUTHORS: Cathy Miller
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>With the advance of science and technology, nanomaterials have been seen in various biomedical applications, ... | [] |
75,344 | A RANDOMIZED, OPEN, CONTROLLED STUDY TO EVALUATE THE EFFICACY AND SAFETY OF PEDIAFLÙ® (DIETARY SUPPLEMENT) ALONG WITH STANDARD OF CARE IN CHILDREN WITH ACUTE TONSILLOPHARYNGITIS / RHINOPHARYNGITIS VERSUS STANDARD OF CARE ONLY | 1 | dx.doi.org/10.17504/protocols.io.kqdg3988eg25/v2 | https://www.protocols.io/view/a-randomized-open-controlled-study-to-evaluate-the-cmtqu6mw | Fabio Cardinale, Dionisio Franco Barattini, Maria Morariu Bordea, Dorina Herteg, Cristian Radu Matei | TITLE: A RANDOMIZED, OPEN, CONTROLLED STUDY TO EVALUATE THE EFFICACY AND SAFETY OF PEDIAFLÙ® (DIETARY SUPPLEMENT) ALONG WITH STANDARD OF CARE IN CHILDREN WITH ACUTE TONSILLOPHARYNGITIS / RHINOPHARYNGITIS VERSUS STANDARD OF CARE ONLY
AUTHORS: Fabio Cardinale, Dionisio Franco Barattini, Maria Morariu Bordea, Dorina Herte... | ["[A RANDOMIZED, OPEN, CONTROLLED STUDY TO EVALUATE THE EFFICACY AND SAFETY OF PEDIAFLÙ® (DIETARY SUPPLEMENT) ALONG WITH STANDARD OF CARE IN CHILDREN WITH ACUTE TONSILLOPHARYNGITIS / RHINOPHARYNGITIS VERSUS STANDARD OF CARE ONLY] A RANDOMIZED, OPEN, CONTROLLED STUDY TO EVALUATE THE EFFICACY AND SAFETY OF PEDIAFLÙ® (DIE... |
92,630 | FAST.R Installation and Launch with RStudio | 4 | null | https://www.protocols.io/view/fast-r-installation-and-launch-with-rstudio-c6pwzdpe | Francesco Neri | TITLE: FAST.R Installation and Launch with RStudio
AUTHORS: Francesco Neri
[DESCRIPTION]
Step -by-step protocol to setup the R shiny application FAST.R for data analysis and visualization of FAST-generated data. This protocol is meant to be used within the FAST workflow
[STEPS]
SECTION: Install FAST.R with RStudio
1.... | ["[Install FAST.R with RStudio] If familiar with R and RStudio, see the FAST.R package page for installation and use.\n\nIf NOT familiar with R and RStudio, read instructions below", "[Install FAST.R with RStudio] Install FAST-R", "[Install FAST.R with RStudio] Download and install R and RStudio\nDownload from here. No... |
22,166 | RNA Isolation for Tissue using TRIzol | null | dx.doi.org/10.17504/protocols.io.zvwf67e | null | Sze-Xian Lim | TITLE: RNA Isolation for Tissue using TRIzol
AUTHORS: Sze-Xian Lim
[STEPS]
?. Add of TRIzol per of tissue and homogenize using handheld homogenizer.
[TRIzol]
[tissue]
?. Incubate at for to allow nucleoprotein complexes to dissociate.
?. Centrifuge at max speed for at .
?. Carefully remove the top aqueous phase and tr... | ["Add of TRIzol per of tissue and homogenize using handheld homogenizer.\n[TRIzol]\n[tissue]", "Incubate at for to allow nucleoprotein complexes to dissociate.", "Centrifuge at max speed for at .", "Carefully remove the top aqueous phase and transfer to a new Eppendorf tube. The interphase and bottom organic phase can... |
98,678 | Western Blot in Mouse Brain Tissue for detecting pRab proteins | 0 | dx.doi.org/10.17504/protocols.io.261ge545jg47/v1 | https://www.protocols.io/view/western-blot-in-mouse-brain-tissue-for-detecting-p-dckw2uxe | madalynn.erb Erb | TITLE: Western Blot in Mouse Brain Tissue for detecting pRab proteins
AUTHORS: madalynn.erb Erb
[DESCRIPTION]
Protocol for detecting pRab proteins in mouse brain tissue
[STEPS]
SECTION: Lysate preparation
1. Freeze freshly dissected brain tissue on dry ice and store at -80 °C
SECTION: Lysate preparation
3. Remove t... | ["[Lysate preparation] Freeze freshly dissected brain tissue on dry ice and store at -80 °C", "[Lysate preparation] Remove tissue from -80 °C and keep on dry ice before starting experiment", "[Lysate preparation] Weigh each tissue sample and record mass", "[Lysate preparation] For regional brain dissections add 1000 µL... |
57,117 | Spin column TNA extraction from plants - GITC method | 4 | dx.doi.org/10.17504/protocols.io.3byl4bkkovo5/v1 | https://www.protocols.click/view/spin-column-tna-extraction-from-plants-gitc-method-b3z5qp86 | James JN Kitson | TITLE: Spin column TNA extraction from plants - GITC method
AUTHORS: James JN Kitson
[DESCRIPTION]
This protocol is designed for extracting total nucleic acids (TNA) from plant material. In reality the drying step probably means that you won't isolate plant mRNA but viral RNA (and probably plant ribosomal RNA) are re... | ["[Initial digestion of OPM larvae] Incubate at 37 °C overnight (12-16 hours)\n\n37 °C", "[Initial digestion of OPM larvae] Centrifuge at 4,000 x g for 2 min.", "[DNA extraction: purification] Add all of the sample solution (~ 600 μL) to a well in a 96-well silica membrane spin column (we use SD5005 from NBS Biological... |
null | null | null | dx.doi.org/10.17504/protocols.io.ky8cxzw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A protocol of Electroacupuncture (EA) treatment on sub-acutely aging rats.</p>
[BEFORE_START]
<p>Sterile acupuncture needles [size:φ0.30*25 mm (diameter:0.30 millimeter, length: 25 millimeter); made by Suzhou Acupuncture ＆ Moxibustion Appliance Co., Lid, Suzhou, P.R. China] ... | [] |
11,390 | cf-RRBS protocol | 1 | null | https://www.protocols.io/view/cf-rrbs-protocol-pc6dize | Andries De Koker, Ruben Van Paemel, Bram De Wilde, Katleen De Preter, Nico Callewaert | TITLE: cf-RRBS protocol
AUTHORS: Andries De Koker, Ruben Van Paemel, Bram De Wilde, Katleen De Preter, Nico Callewaert
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The methylation profile of circulating cell-free DNA (cfDNA) in blood can be exploited to detect and diagnose cancer and other tissue... | ["[Lambda spike-in and dephosporylation]\nAdd to Eppendorf tube:Unmethylated lambda DNA (0.01 ng/µL, 0.1 %w/w): 1 µL or 0.5 µL depending on the DNA input (prepare by going from 10 ng/µL stock to 1 ng/µL to 0.1 ng/µL to 0.01 ng/µL)Recombinant Shrimp Alkaline Phosphatase (rSAP, NEB) (1U/µL): 1 µL10X CutSmart buffer: 1.4 ... |
91,093 | protocols.io academic and non-profit contract | 1 | dx.doi.org/10.17504/protocols.io.e6nvwk5j7vmk/v7 | https://www.protocols.io/view/protocols-io-academic-and-non-profit-contract-c47vyzn6 | protocols.io team | TITLE: protocols.io academic and non-profit contract
AUTHORS: protocols.io team
[DESCRIPTION]
Template Contract
This contract is hereby made between the UNIVERSITY/ORGANIZATION (Univ) and ZappyLab, Inc. (DBA “protocols.io”).
DATE:December 13, 2021START DATE:December 27, 2021END DATE:December 26, 2022DESCRIPTION OF S... | ["[Activation] Within thirty (30) days from the Start Date, Univ will provide to protocols.io a list of IP addresses for on-campus user notification about free Premium workspaces.", "[Activation] Within two (2) weeks from the Start Date, protocols.io will enable free Premium workspaces accounts to be created by any Uni... |
17,093 | CUESTIONARIO PARA PARTICIPANTES EN PROGRAMAS DE INTERVENCIÓN SOCIOLABORAL | null | dx.doi.org/10.17504/protocols.io.uxdexi6 | null | Mª José Gómez Torres, Javier Rodríguez santero, Javier Gil Flores | TITLE: CUESTIONARIO PARA PARTICIPANTES EN PROGRAMAS DE INTERVENCIÓN SOCIOLABORAL
AUTHORS: Mª José Gómez Torres, Javier Rodríguez santero, Javier Gil Flores
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS] | [] |
24,253 | Publication Trends in Drug Delivery and Magnetic Nanoparticles | null | dx.doi.org/10.17504/protocols.io.3w5gpg6 | null | Saba Ale Ebrahim, Amirhossein Ashtari, Maysam Zamani Pedram, Nader Ale Ebrahim | TITLE: Publication Trends in Drug Delivery and Magnetic Nanoparticles
AUTHORS: Saba Ale Ebrahim, Amirhossein Ashtari, Maysam Zamani Pedram, Nader Ale Ebrahim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This bibliometric study investigated the public trends in the fields of nanoparticles which is... | [] |
5,557 | Illumina (post-MR DNA) processing pipeline : mothur | null | dx.doi.org/10.17504/protocols.io.hnvb5e6 | null | Kylie Langlois | TITLE: Illumina (post-MR DNA) processing pipeline : mothur
AUTHORS: Kylie Langlois
[DESCRIPTION]
<p>This pipeline is for datasets generated from Illumina (2x300) sequencing by MR DNA. MR DNA runs sequences through a proprietary pipeline for quality control of sequences and OTU clustering. This pipeline begins with the... | ["[Before you start this protocol]\nBegin with Illumina (post-MR DNA) processing pipeline : QIIME steps 1-7 to rarefy the OTU abundance table. Complete step 12 to convert the rarefied OTU abundance table to a text file.", "[Assign taxonomy]\nTo compare the taxonomy assigned by the sequencing facility (MR DNA in our cas... |
null | null | null | dx.doi.org/10.17504/protocols.io.sfuebnw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>With the Fall Creators update to <strong>Windows 10</strong> comes the ability for all to install <strong>Ubuntu</strong> on your Windows 10 machine by simply downloading the app from the official <a href="https://www.microsoft.com/en-gb/store/p/ubuntu/9nblggh4msv6" target="_... | [] |
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