id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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44,762 | PMN- 06 - Culture of Human PMN - TNF-α production | 4 | dx.doi.org/10.17504/protocols.io.bpx2mpqe | https://www.protocols.io/view/pmn-06-culture-of-human-pmn-tnf-production-bpx2mpqe | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | TITLE: PMN- 06 - Culture of Human PMN - TNF-α production
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Published work using this protocol:</div><div class = "text-block">... | ["Isolate PMN according to the protocol PMN-01a or PMN-01b.", "Resuspend PMN at 10x106cells/ml in RPMI medium supplemented with 10% FBS and 1% penicillin/streptomycin (SOLUTION- 13).", "Add of cell suspension in a 5 ml tube for cell culture and incubate for at in 5% CO2. Cells were incubated in resting condition or ... |
79,825 | Western Blot Analysis | 1 | dx.doi.org/10.17504/protocols.io.j8nlkwp25l5r/v1 | https://www.protocols.click/view/western-blot-analysis-cr7rv9m6 | michela.deleidi, Bianca Marchetti, Federico Bertoli, Carmela Giachino | TITLE: Western Blot Analysis
AUTHORS: michela.deleidi, Bianca Marchetti, Federico Bertoli, Carmela Giachino
[DESCRIPTION]
Western Blotting is a technique for the immunodetection of proteins using antibodies with fluorescent or chemiluminescent detection.
[STEPS]
SECTION: Western Blot Analysis
1. Prepare protein extra... | ["[Western Blot Analysis] Prepare protein extracts as previously described.", "[Western Blot Analysis] Homogenize tissues in lysis buffer (0.33 Molarity (M) sucrose/8 millimolar (mM) Hepes, pH 7.4 and protease inhibitors) and quantify them using the BCA protein determination method (Bio-Rad, Hercules, CA).", "[Western ... |
29,549 | PAS Stain | null | dx.doi.org/10.17504/protocols.io.84mhyu6 | null | Amanda Knoten, sanjay jain | TITLE: PAS Stain
AUTHORS: Amanda Knoten, sanjay jain
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol for PAS Staining of paraffin embedded or cryo preserved tissue sections</div></div>
[STEPS]
?. Deparaffinize and hydrate to water
?. Oxidize in periodic acid solution for 5 minute... | ["Deparaffinize and hydrate to water", "Oxidize in periodic acid solution for 5 minutes", "Rinse in distilled water", "Place in Coleman’s Schiff reagent for 15 minutes", "Rinse in running tap water for 10 minutes", "Counterstain in Harris Hematoxylin for 3 minutes", "Wash in running tap water until water runs clear", "... |
58,918 | Overall protocol for top-down CZE-MS/MS of human heart tissue | 1 | dx.doi.org/10.17504/protocols.io.b5seq6be | https://www.protocols.io/view/overall-protocol-for-top-down-cze-ms-ms-of-human-h-b5seq6be | Jeannie Camarillo, Bryon Drown, Neil Kelleher | TITLE: Overall protocol for top-down CZE-MS/MS of human heart tissue
AUTHORS: Jeannie Camarillo, Bryon Drown, Neil Kelleher
[DESCRIPTION]
Overview description of the process for acquiring top-down CZE-MS/MS data on human heart tissue
[STEPS]
SECTION: Tissue Collection
1. Heart tissue was provided by University of ... | ["[Tissue Collection] Heart tissue was provided by University of Washington. Tissue was collected and prepared according to the following:", "[Sample Preparation] Perform LC based Proteomics on adjacent tissue sections:", "[Data Acquisition]", "[Sample Preparation]"] |
53,679 | Sterilizing the Surface of Seeds by Chlorine Gas | 4 | null | https://www.protocols.io/view/sterilizing-the-surface-of-seeds-by-chlorine-gas-bynppvdn | Lynn Doran | TITLE: Sterilizing the Surface of Seeds by Chlorine Gas
AUTHORS: Lynn Doran
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol sterilizes the surface of seeds for use in clean culture conditions without damaging the seeds germination or growth ability.</div></div>
[STEPS]
?. Move an ali... | ["Move an aliquot of seeds to be sterilized to a 2 mL microcentrifuge tube. Label clearly.\nIf too many seeds are sterilized at once, seeds on the bottom layers may not be exposed to as much gas as top layers.", "Document the seed vials identifications and order via photograph or notetaking.\nExposure to chlorine gas ... |
43,328 | two layer plating method | 1 | dx.doi.org/10.17504/protocols.io.bni8mchw | https://www.protocols.io/view/two-layer-plating-method-bni8mchw | Jiaxin Li | TITLE: two layer plating method
AUTHORS: Jiaxin Li
[STEPS]
?. [BEFORE STARTING]
Prepare 6 cm diameter petri plates containing solidified LB-agar and dry it in the intercellular incubator for 12 h.LB medium formulation： AB1NaCl10g2TRYPTONE10g3YEAST EXTRACT5g4Agar15gTake equipping 1L as an example, other volume isometr... | ["[BEFORE STARTING]\nPrepare 6 cm diameter petri plates containing solidified LB-agar and dry it in the intercellular incubator for 12 h.LB medium formulation： AB1NaCl10g2TRYPTONE10g3YEAST EXTRACT5g4Agar15gTake equipping 1L as an example, other volume isometric adjustmentAntibiotic concentration: ABCD1AbbreviationNam... |
35,593 | 10% Triton X-100 | null | dx.doi.org/10.17504/protocols.io.bezhjf36 | null | Allen Institute for Brain Science | TITLE: 10% Triton X-100
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to attain 10% Triton X-100 which is utilized in several Immunohistochemistry (IHC) reagents to reduce the surface tension of the reagents and to make the cell memb... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kqgcvtw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.g7sbzne | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A simple 96-well plate assay for stress resistance across a range of stress doses. Originally designed for yeast, but easily modifiable for any colony-forming microbe.</p>
[GUIDELINES]
<p>Stress assays are very sensitive to differences in growth conditions. In our hands, we ... | [] |
43,979 | Protocols for "Draft genome of the aquatic moss Fontinalis antipyretica (Fontinalaceae, Bryophyta)" | 2 | dx.doi.org/10.17504/protocols.io.bn7jmhkn | https://www.protocols.io/view/protocols-for-34-draft-genome-of-the-aquatic-moss-bn7jmhkn | Yang Liu, Huan Liu, Hongfeng Chen, Bernard Goffinet, Nikisha Patel, Ziqiang Chen, Shanshan Dong, Sibo Wang, Linzhou Li, Jin Yu | TITLE: Protocols for "Draft genome of the aquatic moss Fontinalis antipyretica (Fontinalaceae, Bryophyta)"
AUTHORS: Yang Liu, Huan Liu, Hongfeng Chen, Bernard Goffinet, Nikisha Patel, Ziqiang Chen, Shanshan Dong, Sibo Wang, Linzhou Li, Jin Yu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span sty... | [] |
41,778 | SARS-CoV-2 Milk ELISA | 4 | dx.doi.org/10.17504/protocols.io.bk2skyee | https://www.protocols.io/view/sars-cov-2-milk-elisa-bk2skyee | Powell RL | TITLE: SARS-CoV-2 Milk ELISA
AUTHORS: Powell RL
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Currently, the COVID-19 diagnostics sector includes Point-of-Care and ELISA tests for IgG and IgM that can assess saliva or blood as described by the CDC [Centers for Disease Control and Prevention]. Simi... | ["[Milk Processing]\nPour fresh milk into 50mL conical tube and centrifuge at 800g for 15min at room temperature\nCentrifuge: 800 34, 15 min, 22 10", "[Milk Processing]\nremove fat using a sterile spatula", "[Milk Processing]\ncarefully pour skim milk into a clean 50mL tube", "[Milk Processing]\nrepeat centrifugation a... |
47,108 | Human pluripotent stem cell culture | 1 | dx.doi.org/10.17504/protocols.io.br9cm92w | https://www.protocols.io/view/human-pluripotent-stem-cell-culture-br9cm92w | Jiuchun Zhang, Harper JW | TITLE: Human pluripotent stem cell culture
AUTHORS: Jiuchun Zhang, Harper JW
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is about human pluripotent stem cell culture.</div><br/><br/><div class = "text-block"><span style = "font-weight:bold;">Some facts about human pluripotent stem ... | ["[Thaw frozen cell from liquid nitrogen tank]\nIMPORTANT, ALWAYS WEAR SAFETY GOGGLES WHEN THAWINGCELLS!!", "[Thaw frozen cell from liquid nitrogen tank]\nTake a vial of frozen cells from liquid nitrogen tank. If you have many vials you need to take out of the tank and thaw at a time, you can put them on dry ice while ... |
36,368 | Microbiome Assay with 96WP Updated April 2020 | null | dx.doi.org/10.17504/protocols.io.bfrqjm5w | https://www.protocols.io/view/microbiome-assay-with-96wp-updated-april-2020-bfrqjm5w | Priota Islam | TITLE: Microbiome Assay with 96WP Updated April 2020
AUTHORS: Priota Islam
[STEPS]
?. [-10 days]
Pick 10 L4s onto 10x90mm OP50 seeded platese.gon Monday (10 L4 per plate so total 100L4s)
?. [-6 days]
At 2pm on day of bleaching (eg Friday) follow the protocol Bleach synchronization of C elegans
?. [-6 days]
Keep the tu... | ["[-10 days]\nPick 10 L4s onto 10x90mm OP50 seeded platese.gon Monday (10 L4 per plate so total 100L4s)", "[-6 days]\nAt 2pm on day of bleaching (eg Friday) follow the protocol Bleach synchronization of C elegans", "[-6 days]\nKeep the tube with bleached N2s on a rotator at 20C incubator until refeed (make sure not to ... |
91,354 | Grip strength test | 1 | dx.doi.org/10.17504/protocols.io.261gedjjwv47/v1 | https://www.protocols.io/view/grip-strength-test-c5f2y3qe | Marina Lorente Picón, Núria Peñuelas, Ariadna Laguna, Miquel Vila | TITLE: Grip strength test
AUTHORS: Marina Lorente Picón, Núria Peñuelas, Ariadna Laguna, Miquel Vila
[DESCRIPTION]
Grip strength test for mice
[STEPS]
1. Hold the animal by the middle/base of the tail and
allow it to grasp a tangled fine gauge stainless steel wire attached to steel
chain (13.2g). Then, lift them carr... | ["Hold the animal by the middle/base of the tail and\nallow it to grasp a tangled fine gauge stainless steel wire attached to steel\nchain (13.2g). Then, lift them carrying the corresponding weight with\ntheir forepaws for a total of 5 seconds. If the animal does not succeed assign 0\nseconds to that animal. If the ani... |
48,553 | Thawing MRC5 sub-stock | 4 | null | https://www.protocols.io/view/thawing-mrc5-sub-stock-btnhnmb6 | Morrisey Lab | TITLE: Thawing MRC5 sub-stock
AUTHORS: Morrisey Lab
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">MRC5 Media</span></div><div class = "text-block">450uls DMFM/F12 (Cell Center cat#11320033)</div><div class = "text-block">45uls 10% FBS (Invitrogen cat#10437-028)</d... | [] |
94,698 | Voltage sensor imaging in a population of dopaminergic axons in the mouse striatum | 1 | dx.doi.org/10.17504/protocols.io.rm7vzxp6rgx1/v1 | https://www.protocols.io/view/voltage-sensor-imaging-in-a-population-of-dopamine-c8qizvue | Lucille Duquenoy, Bethan O'Connor, Stephanie J Cragg | TITLE: Voltage sensor imaging in a population of dopaminergic axons in the mouse striatum
AUTHORS: Lucille Duquenoy, Bethan O'Connor, Stephanie J Cragg
[DESCRIPTION]
This protocol describes how to perform voltage sensor imaging from a population of striatal dopaminergic axons using high frame rates (600 Hz minimum) on... | ["[Brain slice preparation] Sacrifice mice using cervical dislocation, exsanguination and decapitation. Quickly open the skull and collect the brain in an ice-cold slicing solution saturated with 95% O2/ 5% CO2, containing (in mM): 194 sucrose, 30 NaCl, 4.5 KCl, 1 MgCl2-6H2O, 26 NaHCO3, 1.2 NaH2PO4, 10 glucose.", "[Bra... |
76,528 | Nuclear Extraction from Tissue for FACS | 4 | dx.doi.org/10.17504/protocols.io.5jyl8j678g2w/v1 | https://www.protocols.io/view/nuclear-extraction-from-tissue-for-facs-cnyqvfvw | anita.adami | TITLE: Nuclear Extraction from Tissue for FACS
AUTHORS: anita.adami
[DESCRIPTION]
This protocol details the process for pre-tissue preparation and tissue preparation for nuclear extraction for FACS.
[BEFORE_START]
Keep everything On ice.
[STEPS]
SECTION: Pre-Tissue Preparation
1. Coat all tubes (centrifugation, samp... | ["[Pre-Tissue Preparation] Coat all tubes (centrifugation, sample and collection tubes) in coating medium (1 % (v/v), filtered).", "[Pre-Tissue Preparation] Cool ultracentrifuge to 4 °C prior to centrifugation", "[Pre-Tissue Preparation] Pre-weigh the DTT needed.", "[Pre-Tissue Preparation] Keep douncers on ice.", "[... |
103,464 | RNA Isolation of Human Synovium and Fat Pad Tissues | 0 | dx.doi.org/10.17504/protocols.io.5qpvokqwxl4o/v1 | https://www.protocols.io/view/rna-isolation-of-human-synovium-and-fat-pad-tissu-dhag32bw | Irene Lorenzo Gomez, Merissa Olmer, Martin Lotz | TITLE: RNA Isolation of Human Synovium and Fat Pad Tissues
AUTHORS: Irene Lorenzo Gomez, Merissa Olmer, Martin Lotz
[DESCRIPTION]
This protocol demonstrates how to perform RNA isolation from AllProtect preserved or fresh snapfrozen human synovium and fat pad tissues.
[STEPS]
SECTION: Tissue Homogenization
2. Precool... | ["[Tissue Homogenization] Precool Benchtop Centrifuge and make sure temperature is at 4℃.", "[Tissue Homogenization] Prepare tools, mortar and pestle, and work surface by spraying down with RNAse Away.", "[Tissue Homogenization] Prepare piece of tissue, weigh out 120-150mg of tissue.", "[Tissue Homogenization] Add 3-4 ... |
29,125 | Research on an Evolutionary Game Model and Simulation of a Cluster Innovation Network Based on Fairness Preference-new manuscript | null | dx.doi.org/10.17504/protocols.io.8pdhvi6 | null | Li Chuanyun, Xia Cao, Ming Chi | TITLE: Research on an Evolutionary Game Model and Simulation of a Cluster Innovation Network Based on Fairness Preference-new manuscript
AUTHORS: Li Chuanyun, Xia Cao, Ming Chi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The cluster innovation network is an important part of regional economic de... | [] |
42,540 | Home isolation in case of possible COVID-19 | 1 | dx.doi.org/10.17504/protocols.io.bmskk6cw | https://www.protocols.io/view/home-isolation-in-case-of-possible-covid-19-bmskk6cw | Lenny Teytelman | TITLE: Home isolation in case of possible COVID-19
AUTHORS: Lenny Teytelman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is our family protocol for isolation in case one of us (parents) has symptoms which could be consistent with COVID-19.</div><div class = "text-block">After a light scare a... | ["At the onset of symptoms, start wearing a mask immediately, whether at home or in the car.", "Schedule a SARS-CoV-2 test", "Person with symptoms isolates in the parents' bedroom with the door always closed.\nMove the folding table, monitor, chair to the kid's bedroom if needed for the other parent's work.", "Wear a m... |
20,310 | Freezing Tissue in OCT using Isopentane | null | dx.doi.org/10.17504/protocols.io.x3wfqpe | null | Liz Tuck | TITLE: Freezing Tissue in OCT using Isopentane
AUTHORS: Liz Tuck
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><h1>Introduction</h1></div><div class = "text-block">This protocol describes how to freeze tissues in OCT using dry-ice cooled isopentane (for downstream cryo-sectioning).</div><div class... | ["On a downflow or fume hood: Place metal container on dry ice and half fill with isopentane.(use enough iso-pentane to completely immerse the tissue pieces).Iso-pentane is extremely flammable and may be fatal if swallowed or enters airways. To be used in a fume-hood or downflow table wearing correct PPE.", "Cool 1 pai... |
68,576 | Transforming yeast | 4 | null | https://www.protocols.io/view/transforming-yeast-ce78thrw | Brian Teague | TITLE: Transforming yeast
AUTHORS: Brian Teague
[DESCRIPTION]
In the same way that we transformed E. coli by making them take up a plasmid, we will transform yeast by inducing them to take up both a plasmid (to cut your genomic location) and some linear DNA to repair that location.
[STEPS]
1. Compute the volu... | ["Compute the volume of your Cas9 plasmid that contains 1 µg of DNA.\nCompute the volume of your URA3 PCR product that contains 500 ng of DNA.\nIf you don't have enough DNA for one or both of these, check with an instructor.", "Immediately upon retrieving a tube of yeast cells from the -80 °C freezer, thaw them rapidly... |
null | null | null | dx.doi.org/10.17504/protocols.io.c28yhv | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
Needed:<br /><ul><li>0.2 µm filtered seawater with iron chloride</li><li>Mg-EDTA-Ascorbate (Oxalate) buffer</li><li>DNase I</li><li>EDTA</li><li>EGTA</li><li>Centrifugal concentrators</li><li>Resuspension buffer</li><li>Wizard prep column</li><li>Wizard prep resin</li><li>Q... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kwwcxfe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Isolated human islets were dispersed into single cells using Accutase (Millipore), ~1500-2000 islet equivalents (IEQ)/ml. 20,000-65,000 islet cells were transferred to individual wells of a 96 well culture plate (CELLSTAR, Greiner Bio-one) for immediate staining for flow cyto... | [] |
70,555 | OT-2 Media dispensing and culture inoculation protocol | 5 | dx.doi.org/10.17504/protocols.io.q26g7yb3kgwz/v1 | https://www.protocols.io/view/ot-2-media-dispensing-and-culture-inoculation-prot-cg53ty8n | Ana Mariya Anhel, Lorea Alejaldre, Ángel Goñi-Moreno | TITLE: OT-2 Media dispensing and culture inoculation protocol
AUTHORS: Ana Mariya Anhel, Lorea Alejaldre, Ángel Goñi-Moreno
[DESCRIPTION]
This protocol is meant to distribute reactive(s) into well plates with a single channel pipette, and then transfer culture samples from a source plate to these plates with a multi c... | ["[Files Preparation] Preparing Customized Template\n\nPreparing the template (a .csv) with the specific variables for each experiment.\n\nHere we attach one excel with several sheets:\nTemplate to use in protocol\nExplanation of each variable\nSeveral examples", "[Run Protocol] Setting Labware", "[After-Running] Retri... |
null | null | null | dx.doi.org/10.17504/protocols.io.q2pdydn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><em><strong>Making a Phylogenetic Tree</strong></em></p>
<p> </p>
<p>In this procedure we are going to build a phylogenetic tree! Throughout I'll refer to scripts available on my github here. We will be using the 16 ribosomal proteins used in Hug et al. 2016. The HMM models f... | [] |
76,857 | PCR reaction of marker regions (a.k.a metabarcodes) for two kingdoms | 4 | null | https://www.protocols.io/view/pcr-reaction-of-marker-regions-a-k-a-metabarcodes-cpazvif6 | Abigail Graetz, Benjamin Schwessinger | TITLE: PCR reaction of marker regions (a.k.a metabarcodes) for two kingdoms
AUTHORS: Abigail Graetz, Benjamin Schwessinger
[DESCRIPTION]
This protocols is part of the ANU Biosecurity mini-research project #1 "Plant Pathogen Diagnostics: Visuals, subcultures, and genomics".
You will be provided four pots of 3-4 week ... | ["[Week 4: DNA quantification and dilution] The first section describes the quantification of extracted DNA from week 3. Once you know the concentration for each TG you can generate a DNA stock solution at a final concentration of 10 ng/ul. This stock solution for each TG will be used for the following PCR reaction and... |
28,609 | Measuring effects of down-regulated genes | null | dx.doi.org/10.17504/protocols.io.769hrh6 | null | iGEM Dusseldorf | TITLE: Measuring effects of down-regulated genes
AUTHORS: iGEM Dusseldorf
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><ul style = "list-style-type:disc;"><li style = "counter-reset:ol0;list-style-type:disc;">Synechocystis sp. PCC 6803 with integrated guide RNA culture</li><li style = "counter-re... | [] |
56,661 | F/2 medium added soil extract from estuary water at 27 PSU of salinity | 4 | dx.doi.org/10.17504/protocols.io.dm6gpbr9plzp/v1 | https://www.protocols.io/view/f-2-medium-added-soil-extract-from-estuary-water-a-b3jvqkn6 | Estelle Bigeard, Laure Guillou | TITLE: F/2 medium added soil extract from estuary water at 27 PSU of salinity
AUTHORS: Estelle Bigeard, Laure Guillou
[DESCRIPTION]
F/2 medium (Guillard’s Marine Water Enrichment Solution) is used to maintain in culture microalgae (for example, Ddinoflagellates) and their parasites (Alveolates, Syndiniales & perkinsid... | ["[Water Sampling] Sea water is sampled in the Penzé Estuary from June to July (during the dinoflagellate blooms), at a salinity of 27 PSU.\n\nThe sea water is stored at dark during three months (minimum two months).", "[Installation and Sterilization Procedures for Filtration System] On the tripod, place :\n- First th... |
null | null | null | dx.doi.org/10.17504/protocols.io.nhzdb76 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<section>
<p><a href="http://bioinformatics.psb.ugent.be/webtools/trapid/" target="_blank">TRAPID</a> is an online tool for the fast, reliable and user-friendly analysis of <em>de novo</em> transcriptomes, developed and maintained by the CNB group at VIB-UGent Center for Plant S... | [] |
52,607 | Coleta de dados actigráficos - ActTrust | 2 | dx.doi.org/10.17504/protocols.io.bxk7pkzn | https://www.protocols.io/view/coleta-de-dados-actigr-ficos-acttrust-bxk7pkzn | Daniel Vartanian | TITLE: Coleta de dados actigráficos - ActTrust
AUTHORS: Daniel Vartanian
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Esta coleção reúne os protocolos do processo de coleta de dados actigráficos do Grupo Interdisciplinar de Pesquisa em Sono (GIPSO). Esses protocolos foram desenhados para os... | [] |
92,741 | Fixed RNA - GEM Recovery - Sequencing | 4 | dx.doi.org/10.17504/protocols.io.3byl4q8ejvo5/v1 | https://www.protocols.io/view/fixed-rna-gem-recovery-sequencing-c6tdzei6 | Ksenija Sabic | TITLE: Fixed RNA - GEM Recovery - Sequencing
AUTHORS: Ksenija Sabic
[DESCRIPTION]
This protocol is a continuation of CG000527 (Step 3 and onward).
[BEFORE_START]
Prepare daily fresh 80% Ethanol for wash steps.
[GUIDELINES]
Please review and consult the full 10x Genomics protocols prior to starting and at any point ... | ["[GEM Recovery and Pre-Amplification] Add 125 µL Recovery Agent to each sample at room temperature. \n\nDO NOT pipette mix or vortex the biphasic mixture.", "[GEM Recovery and Pre-Amplification] Firmly secure the cap on the tube strip, ensuring that no liquid is trapped between the cap and the tube rim. Mix by inverti... |
57,305 | Examining Health Conditions, Body Functions, Activity and Participation, and Quality of Life among Adults with Learning Disabilities – Towards a Theoretical Model | 1 | dx.doi.org/10.17504/protocols.io.b37zqrp6 | https://www.protocols.io/view/examining-health-conditions-body-functions-activit-b37zqrp6 | Kineret Sharfi, Sara Rosenblum | TITLE: Examining Health Conditions, Body Functions, Activity and Participation, and Quality of Life among Adults with Learning Disabilities – Towards a Theoretical Model
AUTHORS: Kineret Sharfi, Sara Rosenblum
[DESCRIPTION]
Background: The term learning disabilities (LD) refers to a heterogeneous group of neurode... | [] |
68,154 | Phylogenomic analysis of Xanthomonas | 5 | null | https://www.protocols.io/view/phylogenomic-analysis-of-xanthomonas-ces2tege | David J Studholme | TITLE: Phylogenomic analysis of Xanthomonas
AUTHORS: David J Studholme
[DESCRIPTION]
This is a protocol for using PhaME to generate a phylogenomic tree from a set of Xanthomonas spp. genome sequences.
[STEPS]
9. Install PhaME software into a Conda environment called 'phame', following instructions on the software's G... | ["Install PhaME software into a Conda environment called 'phame', following instructions on the software's GitHub page:\n \nThe PhaME software is described in this paper:", "Set-up the reference genome sequence data", "Set-up the working directory.\n\n \n\n \n\n\n \nOptionally, at this point, we can delete the symbolic... |
37,756 | 0.9% Saline Solution | 1 | dx.doi.org/10.17504/protocols.io.bg44jyyw | https://www.protocols.io/view/0-9-saline-solution-bg44jyyw | Allen Institute for Brain Science | TITLE: 0.9% Saline Solution
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to prepare 0.9% Saline Solution. 0.9% Sterile Saline solution is used as an initial rinse for the brains of mice prior to perfusion. </div><div class = "text-b... | [] |
63,079 | Quantitative Method to Measure Disinfectant Product Efficacy | 4 | dx.doi.org/10.17504/protocols.io.kxygxz13zv8j/v2 | https://www.protocols.io/view/quantitative-method-to-measure-disinfectant-produc-b9ufr6tn | John Hilgren | TITLE: Quantitative Method to Measure Disinfectant Product Efficacy
AUTHORS: John Hilgren
[DESCRIPTION]
This protocol is used for measuring the inactivation of Pseudomonas aeruginosa by liquid disinfectants. In brief, stainless-steel discs are inoculated with single 10 µL drops of a P. aeruginosa suspension also conta... | ["[Prepare vials of frozen stock culture] Prepare vials of frozen stock culture", "[Prepare vials of frozen stock culture] Mix thoroughly.", "[Prepare vials of frozen stock culture] Incubate broth culture at 36°C ± 1°C for 24 h ±2 h.", "[Prepare vials of frozen stock culture] Streak a loopful of the broth culture onto ... |
null | null | null | dx.doi.org/10.17504/protocols.io.qwudxew | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Protocol for measurement of carbon isotope descrimination during photosynthesis.</p>
<p> </p>
<p>See the following references for more background:</p>
<p>H. Griffiths (1993) <a href="https://link.springer.com/book/10.1007/978-94-011-1566-7" target="_blank" rel="noopener noref... | [] |
90,664 | Visualign Transformation | 4 | dx.doi.org/10.17504/protocols.io.5qpvo38odv4o/v1 | https://www.protocols.io/view/visualign-transformation-c4sgywbw | Michael X. Henderson | TITLE: Visualign Transformation
AUTHORS: Michael X. Henderson
[DESCRIPTION]
This protocol describes visualign transformation.
[STEPS]
SECTION: Visualign Transformation
1. Open Visualign program from folder on the desktop.
SECTION: Visualign Transformation
2. File > Open > select “QuickN JSON” you created in QuickNII... | ["[Visualign Transformation] Open Visualign program from folder on the desktop.", "[Visualign Transformation] File > Open > select “QuickN JSON” you created in QuickNII.", "[Visualign Transformation] Drag the opacity bar all the way to the right to where it says outline, and you will have all outlined regions displayed... |
59,313 | Building plan for a temperature-controlled multi-point stirring incubator | 1 | dx.doi.org/10.17504/protocols.io.dm6gpb34dlzp/v1 | https://www.protocols.io/view/building-plan-for-a-temperature-controlled-multi-p-b56rq9d6 | Marvin Rades, Patrick Schubert, Maren Ziegler, Martin Kröckel, Jessica Reichert | TITLE: Building plan for a temperature-controlled multi-point stirring incubator
AUTHORS: Marvin Rades, Patrick Schubert, Maren Ziegler, Martin Kröckel, Jessica Reichert
[DESCRIPTION]
Multiple point stirrers are expensive lab equipment (usually €1,000–4,000) and are only offered by a handful of manufacturers. Mor... | ["[Wooden construction] The plywood panel and the wooden slats can be bought with the desired dimensions according to the number of stir points (e.g., 65 x 50 x 1.2 cm for 12 stir points)", "[Wooden construction] The wooden slats are mounted on the edge of the coated side of the plywood panel with wood screws. Drill an... |
14,799 | Immunohistochemistry | 1 | dx.doi.org/10.17504/protocols.io.sppedmn | https://www.protocols.io/view/immunohistochemistry-sppedmn | Kristin Anderson, Sunni Farley | TITLE: Immunohistochemistry
AUTHORS: Kristin Anderson, Sunni Farley
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol outlines the steps used to perform immunohistochemistry on murine and human tumor samples.</div><div class = "text-block">In brief: Study animals underwent full necropsie... | ["[Grossing and specimen processing]\nGross one specimen at a time at the Mopec grossing station.Carefully verify the cassette information to the specimen ID on the specimen container.All contaminated biohazard bags must be disposed of in the approved biohazard containers.Load the cassette holding rack into Sakura Tiss... |
74,514 | BIDMC TMC - SOP for collection of peripheral lymphatic vessels | 1 | dx.doi.org/10.17504/protocols.io.e6nvwjeb7lmk/v1 | https://www.protocols.io/view/bidmc-tmc-sop-for-collection-of-peripheral-lymphat-ckzsux6e | nkalavro, Ioannis Vlachos, Dhruv Singhal | TITLE: BIDMC TMC - SOP for collection of peripheral lymphatic vessels
AUTHORS: nkalavro, Ioannis Vlachos, Dhruv Singhal
[DESCRIPTION]
BIDMC Lymphatic TMC SOP for peripheral lymphatic vessel collection
[STEPS]
SECTION: Standard Operating Procedure Overview
1. Standard Operating Procedure to outline the process for obt... | ["[Standard Operating Procedure Overview] Standard Operating Procedure to outline the process for obtaining, processing, and storing peripheral lymphatic vessel samples collected for the HuBMAP Lymphatic TMC.\n\nThe vessels are collected through a permanent removal of right axillary lymphatic vessel during lymphedema s... |
18,633 | Native bee biodiversity long-term monitoring/survey/inventory protocol | null | dx.doi.org/10.17504/protocols.io.wfhfbj6 | null | Joan Meiners | TITLE: Native bee biodiversity long-term monitoring/survey/inventory protocol
AUTHORS: Joan Meiners
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is the full sampling protocol for a long-term native bee biodiversity monitoring/survey/inventory set up in 2011 at Pinnacles National Park in Cali... | ["[Set up long-term monitoring sites]\nSelect sites:We began our study by scouting out locations within our study area that represent a variety of habitat types so that we could survey all the different major landscapes within our broader area of interest (in this case, Pinnacles National Park in California). We looked... |
null | null | null | dx.doi.org/10.17504/protocols.io.jm3ck8n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Isolation of 40-200 µg of total RNA from <em>Synechocystis</em> sp. PCC 6803. Can be used for standard applications such as Northern Blot and qPCR.</p>
[BEFORE_START]
<p>PGTX solution (100 mL): 39.6 g phenol, 6.9 mL glycerol, 0.1 g hydroxyquinoline, 0.58 g EDTA, 0.8 g NaOAc,... | [] |
62,444 | TSS-MPRA Protocol | 4 | dx.doi.org/10.17504/protocols.io.kqdg3p7kzl25/v1 | https://www.protocols.io/view/tss-mpra-protocol-b88krzuw | Carlos Guzman, Sascha Duttke, Camila De Arruda Saldanha, Christopher Benner, Sven Heinz | TITLE: TSS-MPRA Protocol
AUTHORS: Carlos Guzman, Sascha Duttke, Camila De Arruda Saldanha, Christopher Benner, Sven Heinz
[DESCRIPTION]
Cis-regulatory elements can be classified by the shapes of their transcription initiation patterns, which are indicative of distinct regulatory mechanisms. While massively parallel re... | ["[RNA Extraction] Add 750 µL of per 250 µL of sample volume (3x) and pipette up and down 5x (Optional: samples can be stored at 4 °C ON or at -20 °Cfor up to a year).", "[RNA Extraction] Incubate at RT for 5 min.", "[RNA Extraction] Add 230 µL of and shake vigorously by hand for 15 s.", "[RNA Extraction] Incubate ... |
65,557 | Generating a gRNA construct for a CRISPR KO experiment | 4 | dx.doi.org/10.17504/protocols.io.81wgb6oeqlpk/v1 | https://www.protocols.io/view/generating-a-grna-construct-for-a-crispr-ko-experi-cb9vsr66 | Goran Tomic | TITLE: Generating a gRNA construct for a CRISPR KO experiment
AUTHORS: Goran Tomic
[DESCRIPTION]
This protocol is designed to clone a gRNA of choice into the PX458 plasmid to nucleofect at a later stage and select single cell clones on the basis of GFP fluorescence. The GFP fluorescence and Cas9 expression are transi... | ["Design suitable gRNA to target exon of interest (Use Benchling or Deskgen tools to generate guide(s))\nBenchling tool can generate oligo sequences that include BbsI sticky ends for cloning into PX458. Order the oligos from Sigma/IDT. Order dry individual oligos and anneal in the lab or order already annealed.\n*If pe... |
35,893 | Modified Nasal Swab For Detection of Sars-Cov2 | null | dx.doi.org/10.17504/protocols.io.bfavjie6 | https://www.protocols.io/view/modified-nasal-swab-for-detection-of-sars-cov2-bfavjie6 | Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton | TITLE: Modified Nasal Swab For Detection of Sars-Cov2
AUTHORS: Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The common methods of sample collection for the detection of SARS-Cov2 are either pharyngeal or nasopharyngeal... | ["[Directions for the individual collecting the sample]\nTransport the cooler and swabbing kit to the site for sample processing and place in a 4ºC fridge or coldroom", "[Directions for the individual collecting the sample]\nPlace the biohazard bag in the cooler on top of cooler packs", "[Directions for the individual ... |
67,408 | https://www.facebook.com/Diaetoxilkapselnavis/ | 1 | dx.doi.org/10.17504/protocols.io.kxygxz6k4v8j/v1 | https://www.protocols.io/view/https-www-facebook-com-diaetoxilkapselnavis-cd3qs8mw | Alexcartersz | TITLE: https://www.facebook.com/Diaetoxilkapselnavis/
AUTHORS: Alexcartersz
[DESCRIPTION]
Diaetoxil Avis: ne personne a besoin d'avoir un corps sain. Peu importe votre richesse financière ou toutes les ressources dont vous disposez, si votre santé est mauvaise, vous attirerez de nombreux problèmes de santé dans votr... | ["[https://www.facebook.com/Diaetoxilkapselnavis/]"] |
51,306 | Human embryonic gonad dissociation with Collagenase & Trypsin | 1 | dx.doi.org/10.17504/protocols.io.bwcipaue | https://www.protocols.io/view/human-embryonic-gonad-dissociation-with-collagenas-bwcipaue | Carmen Sancho, Regina Hoo, Roser Vento-Tormo | TITLE: Human embryonic gonad dissociation with Collagenase & Trypsin
AUTHORS: Carmen Sancho, Regina Hoo, Roser Vento-Tormo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for enrichment of fetal gonadal cells</div></div>
[STEPS]
?. [Prepare collagenase mix]
Heat-inactivate FBS at ... | ["[Prepare collagenase mix]\nHeat-inactivate FBS at for before use.Collagenase mix recipe: ABCD1ProductStockFinal volume (10 ml)Concentration2RPMI or Hams F12\n+ 10% FBS9 ml RPMI or Hams F12\n+ 1ml FBS8.8 ml3Collagenase IA10 mg/ml1 ml1 mg/ml4Liberase TM5 mg/ml100 ul50 ug/ml5DNase I10 mg/ml100 ul0.1 mg/ml\n56 °C\nABC... |
41,671 | Sequencing Protocol (GenapSys) | 1 | dx.doi.org/10.17504/protocols.io.bkxfkxjn | https://www.protocols.io/view/sequencing-protocol-genapsys-bkxfkxjn | Sid Roy | TITLE: Sequencing Protocol (GenapSys)
AUTHORS: Sid Roy
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">User guide to prepare your libraries for sequencing on the GenapSys Sequencer.</div></div>
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.r65d9g6 | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<p>Keeps cells always on ice & all reagents must be ice-cold</p>
<p> </p>
[STEPS]
SECTION: Start a culture two days before transfection to get the culture full of amoebas cells
?.
SECTION: Count the cells to reach 1.5E6 cells / condition
?.
SECTION: Spin cells down at 2500... | ["[Start a culture two days before transfection to get the culture full of amoebas cells] 300ul of culture + 9ml of Marine Broth in a 25cm2 flask", "[Count the cells to reach 1.5E6 cells / condition] If there are more conditions, it is recommended to pull the cells together to get more visible pellet", "[Spin cells dow... |
67,676 | Injection of algae into Ambystomid (salamander) embryo | 4 | dx.doi.org/10.17504/protocols.io.5jyl89y17v2w/v1 | https://www.protocols.io/view/injection-of-algae-into-ambystomid-salamander-embr-ceb4taqw | John Burns, Hui Yang, Joost S Mansour | TITLE: Injection of algae into Ambystomid (salamander) embryo
AUTHORS: John Burns, Hui Yang, Joost S Mansour
[DESCRIPTION]
This protocol describes the steps taken to artificially introduce algal cells into salamander tissues by microinjection for the study of salamander-alga endosymbiosis. Microinjection allows expe... | ["[Prepare the embryos] Load whole eggs containing embryos into a strainer.", "[Prepare the embryos] Wash eggs with 70% ethanol for ~20 seconds.", "[Prepare the embryos] Wash eggs with Milli-Q water for ~20 seconds.", "[Prepare the embryos] Following the water rinse, transfer eggs to a 100 mm petri dish containing ster... |
51,238 | Covid Apps | 1 | dx.doi.org/10.17504/protocols.io.bwaepabe | https://www.protocols.io/view/covid-apps-bwaepabe | Asako Chiba | TITLE: Covid Apps
AUTHORS: Asako Chiba
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This page provides codes that are used in "Modeling the effects of contact-tracing apps on the spread of the coronavirus disease: mechanisms, conditions, and efficiency."</div></div>
[STEPS]
?. | [] |
57,412 | Accelerated Ovarian Failure AOF mouse model: a model of transitional menopause | 4 | dx.doi.org/10.17504/protocols.io.yxmvmnkbng3p/v1 | https://www.protocols.io/view/accelerated-ovarian-failure-aof-mouse-model-a-mode-b4bcqsiw | Roberta Marongiu | TITLE: Accelerated Ovarian Failure AOF mouse model: a model of transitional menopause
AUTHORS: Roberta Marongiu
[DESCRIPTION]
Until recently, aging and ovariectomy (OVX) have been the primary rodent models available to examine effects of ovarian hormone loss. These models fail to adequately recapitulate the hormonal ... | ["[Ovotoxin 4-vinylcyclohexene diepoxide (VDC) preparation] Determine the number of mice to treat with VCD to calculate how much VCD is needed each day.", "[Ovotoxin 4-vinylcyclohexene diepoxide (VDC) preparation] Weigh all the mice and obtain an average weight at least once a week for the duration of the VCD treatment... |
47,896 | Biospecimen collection and processing 2.0 | 1 | dx.doi.org/10.17504/protocols.io.bszynf7w | https://www.protocols.io/view/biospecimen-collection-and-processing-2-0-bszynf7w | John Herndon, Ryan Fields, Daniel Cui Zhou, Li Ding | TITLE: Biospecimen collection and processing 2.0
AUTHORS: John Herndon, Ryan Fields, Daniel Cui Zhou, Li Ding
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">ABSTRACT</div><div class = "text-block">This is a description of the steps that the Washington University in St. Louis HTAN center has develop... | ["Record time that the tumor sample arrived in the pathology gross lab", "Examine the gross tumor sample with a Pathologist or Pathology Assistant", "Once the tumor mass has been identified, photograph the tumor in situ", "Request that the Pathologist remove a tumor piece that is ideally 2.0 cm2 x 0.5cm thick.", "Reque... |
null | null | null | dx.doi.org/10.17504/protocols.io.e4fbgtn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>1µl Assay for detecting and measuring DNA, RNA & Oligos.</p>
[GUIDELINES]
<p><strong>INTRODUCTION </strong></p>
<p>The patented NUCLEIC dotMETRIC™ kit provides a quick and accurate method for detecting and measuring DNA, RNA and oligonucleotides. This system is an alter... | [] |
48,810 | Protocol for "Characterization of projections of long interneurons in human colon" - Brookes Lab | 1 | dx.doi.org/10.17504/protocols.io.btwinpce | https://www.protocols.io/view/protocol-for-34-characterization-of-projections-of-btwinpce | Simon Brookes, Bao Nan Chen, Adam Humenick | TITLE: Protocol for "Characterization of projections of long interneurons in human colon" - Brookes Lab
AUTHORS: Simon Brookes, Bao Nan Chen, Adam Humenick
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol outlines the basic methods for retrograde tracing of enteric neuronal pathways i... | ["[Preparation]\nIn weeks prior to the experiment, coat a batch of small glass beads (500 μm diameter - Sigma) with 1,1'‐didodecyl‐3,3,3',3'‐tetramethylindocarbocyanine perchlorate (DiI - Invitrogen) dissolved in ethanol.", "[Preparation]\nMake up sterile culture medium (Dulbecco’s Modified Eagle’s Medium/Nutrient Mix ... |
null | null | null | dx.doi.org/10.17504/protocols.io.s5peg5n | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
50,466 | Making no peptone NGM for imaging plates | 1 | dx.doi.org/10.17504/protocols.io.bvian4ae | https://www.protocols.io/view/making-no-peptone-ngm-for-imaging-plates-bvian4ae | Bonnie Evans | TITLE: Making no peptone NGM for imaging plates
AUTHORS: Bonnie Evans
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-style:italic;">C. elegans</span><span> is maintained in the laboratory on Nematode Growth Medium (NGM) agar seeded with bacteria. For imaging plates, NGM without ... | ["[Pre-autoclave]\nBook the autoclave via the notebook on top of the machine. It will take around 2 hours for a 500ml bottle and 2.5 hours for 1L or larger bottles.", "[Pre-autoclave]\nTake clean bottles and measuring cylinders from the glass kitchen - you will also need one bottle for the autoclave probe, which should... |
null | null | null | dx.doi.org/10.17504/protocols.io.kbbcsin | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span lang="EN-US" style="margin: 0px; line-height: 200%; font-family: 'Arial',sans-serif; font-size: 10pt;">Increasing energy expenditure by stimulating thermogenesis through activation of brown adipose tissue (BAT) and/or induction of browning of white adipose tissue (WAT) ... | [] |
46,258 | Environmental sampling using Moore swabs | 4 | null | https://www.protocols.io/view/environmental-sampling-using-moore-swabs-bresm3ee | Dilip Abraham, Nicola Elviss, Nicholas Feasey, Nick Grassly, Jacob John, Gagandeep Kang, John Scott Meschke, Venkata Raghava Mohan, Satheesh Nair, Jonathan Rigby, Rajan Srinivasan, Catherine Troman, Christopher Uzzell, Nicolette Zhou | TITLE: Environmental sampling using Moore swabs
AUTHORS: Dilip Abraham, Nicola Elviss, Nicholas Feasey, Nick Grassly, Jacob John, Gagandeep Kang, John Scott Meschke, Venkata Raghava Mohan, Satheesh Nair, Jonathan Rigby, Rajan Srinivasan, Catherine Troman, Christopher Uzzell, Nicolette Zhou
[DESCRIPTION]
A proto... | ["[Sample Collection] Sterilise Moore swabs by autoclaving prior to taking them to the field site.", "[Sample Collection] Immerse the swab in the sewage or wastewater channel and tie it to a stable support using the tailing thread of the swab.", "[Sample Collection] Leave in place for 24-72 hours", "[Sample Collection]... |
28,037 | Tissue Freezing for seqFISH | null | dx.doi.org/10.17504/protocols.io.7mdhk26 | null | Nina Dar, Long Cai | TITLE: Tissue Freezing for seqFISH
AUTHORS: Nina Dar, Long Cai
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Once a tissue is received, it should be frozen using the following protocol: </div></div>
[STEPS] | [] |
46,685 | 10Xv3.1 Genomics Sample Processing | 1 | dx.doi.org/10.17504/protocols.io.brt5m6q6 | https://www.protocols.io/view/10xv3-1-genomics-sample-processing-brt5m6q6 | Allen Institute for Brain Science | TITLE: 10Xv3.1 Genomics Sample Processing
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used for the rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells.</div><div class = "text-block"><span style = ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.indcda6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol allows for adequate DNA extraction from archival samples.</p>
[BEFORE_START]
<p>Separate PCR-free facility</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
51,609 | phi29 DNA polymerase protocol | 4 | dx.doi.org/10.17504/protocols.io.bwmzpc76 | https://www.protocols.io/view/phi29-dna-polymerase-protocol-bwmzpc76 | Elena Hilario | TITLE: phi29 DNA polymerase protocol
AUTHORS: Elena Hilario
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Isothermal amplification of a few nanograms of DNA with phi29 DNA polymerase in the presence of trehalose allows for a more stringent amplification temperature that prevents unspecific primer ... | ["[Isothermal amplification]\nSet up the following profile in a PCR machine, 1 cycle only, lid at 105ºC:95ºC 3 min → 40ºC 1 min → 38ºC 12.5 h → 65ºC 10 min → 10ºC 10 min → stop", "[Isothermal amplification]\nPrepare the 1X amplification mix and keep it on ice. Besides the DNA reaction, include a water control, and an e... |
20,484 | iPSC Cortical Differentiation | null | dx.doi.org/10.17504/protocols.io.x9cfr2w | null | Celeste Karch, Rita Martinez, Jacob Marsh | TITLE: iPSC Cortical Differentiation
AUTHORS: Celeste Karch, Rita Martinez, Jacob Marsh
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">All methods described in this collection should be performed using sterile technique. </div></div>
[STEPS] | [] |
49,356 | BC derivatization for dissolved marine metabolites | 6 | null | https://www.protocols.io/view/bc-derivatization-for-dissolved-marine-metabolites-bufkntkw | Brittany Widner, Melissa Soule, Frank Ferrer-Gonzalez, Mary Ann Moran, Elizabeth Kujawinski | TITLE: BC derivatization for dissolved marine metabolites
AUTHORS: Brittany Widner, Melissa Soule, Frank Ferrer-Gonzalez, Mary Ann Moran, Elizabeth Kujawinski
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Benzoyl chloride derivatization of dissolved metabolites in seawater and culture medium impr... | ["[Prepare Standards for 1 mL and 25 mL methods]\nStandards for samples (culture samples)\n1 mL", "[Prepare Standards for 1 mL and 25 mL methods]\n1ostocks: mixes 1, 2, 3, 4, 5-6, and thiol* (optional).", "[Prepare Standards for 1 mL and 25 mL methods]\n2ostock:mix of all metabolites. 1:100 dilution of primary into mat... |
82,271 | Slide Preparation and Transect Counting of Paleoecological Microfossils - SPaTCoPM | 1 | dx.doi.org/10.17504/protocols.io.eq2ly7ormlx9/v1 | https://www.protocols.io/view/slide-preparation-and-transect-counting-of-paleoec-cuj7wurn | Megan C. O'Toole, Richa Patel, Jacopo Niccolo Cerasoni | TITLE: Slide Preparation and Transect Counting of Paleoecological Microfossils - SPaTCoPM
AUTHORS: Megan C. O'Toole, Richa Patel, Jacopo Niccolo Cerasoni
[DESCRIPTION]
Paleoecology attempts to reconstruct history through geochemical isotopic studies, trace fossil analyses, examination of microbe communities, and the p... | ["[Slide Preparation] Using a needle tool, grind the sample inside the falcon tube to create a fine powder.", "[Microscopy (Brightfield) and Transect Counting] Use a compound microscope, binocular or trinocular, for brightfield viewing (light placed below the sample, viewing from above).", "[Microscopy (Brightfield) an... |
21,167 | Cell sorting of marine heterotrophic flagellates for single-cell genome amplification | null | dx.doi.org/10.17504/protocols.io.ywpfxdn | null | Camille Poirier, Raquel Rodríguez‐Martínez, Emily Cook, David S. Milner, Alexandra Z. Worden, Thomas A. Richards | TITLE: Cell sorting of marine heterotrophic flagellates for single-cell genome amplification
AUTHORS: Camille Poirier, Raquel Rodríguez‐Martínez, Emily Cook, David S. Milner, Alexandra Z. Worden, Thomas A. Richards
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Water collection and preparation]
Gently re... | ["[Water collection and preparation]\nGently resuspend the cells from the filter using 1-2 ml sterile artificial sea water (ASW) and stain a 500 µl volume of the cell concentrate with 10 µM Paclitaxel, Oregon Green® 488 Conjugate (to target cytoskeletal tubulin). Incubate for 1 hour at room temperature.\nUse a 1:100 di... |
57,369 | AAV Purification Protocol with Iodixanol Gradient | 4 | dx.doi.org/10.17504/protocols.io.ewov1n4x2gr2/v1 | https://www.protocols.io/view/aav-purification-protocol-with-iodixanol-gradient-b39zqr76 | Roberta Marongiu | TITLE: AAV Purification Protocol with Iodixanol Gradient
AUTHORS: Roberta Marongiu
[DESCRIPTION]
Protocol used in the Kaplitt and Marongiu labs to purify AAVs.
[STEPS]
SECTION: Transfection
1. Plate 293 cells in CellSTACK double chamber in 200 mLmedia (DMEM with 10% FBS and 1% Penicillin/Streptomycin) so that at the ... | ["[Transfection] Plate 293 cells in CellSTACK double chamber in 200 mLmedia (DMEM with 10% FBS and 1% Penicillin/Streptomycin) so that at the moment of transfection they reach 80% confluence.", "[Transfection] When the cells reach 80% confluence, they are ready to be transfected. While preparing the transfection reagen... |
92,523 | Osmia lignaria laboratory rearing protocol | 1 | dx.doi.org/10.17504/protocols.io.eq2lyj4qplx9/v1 | https://www.protocols.io/view/osmia-lignaria-laboratory-rearing-protocol-c6kjzcun | Mary-Kate F. Williams, Natalie K. Boyle, Robert N. Schaeffer, Diana L. Cox-Foster | TITLE: Osmia lignaria laboratory rearing protocol
AUTHORS: Mary-Kate F. Williams, Natalie K. Boyle, Robert N. Schaeffer, Diana L. Cox-Foster
[DESCRIPTION]
Our protocol was designed to rear Osmia lignaria Say (Hymenoptera: Megachilidae) from immature stages to adult emergence following their natural phenology in northe... | ["[Propagation and acquiring solitary bee nests (Fig. 1)] Deploy corrugated plastic nesting boxes (Artz et al. 2013, 2014) into the orchard before bloom. Place nest blocks with straw inserts or bundles of cardboard tubes with straw inserts into nest boxes.", "[Propagation and acquiring solitary bee nests (Fig. 1)] Once... |
22,104 | Sample Preparation for 3D-Raman Microspectroscopic Mapping of Fully Hydrated Protist Cells | null | dx.doi.org/10.17504/protocols.io.ztyf6pw | null | Felix Weber, Tatiana Zaliznyak, Gordon Taylor | TITLE: Sample Preparation for 3D-Raman Microspectroscopic Mapping of Fully Hydrated Protist Cells
AUTHORS: Felix Weber, Tatiana Zaliznyak, Gordon Taylor
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span> Sample preparation for Raman Microspectroscopic analysis of microbial cells mostly requir... | ["[Washing steps]\nCentrifugation of formaldehyde-fixed sample at 150 rcf for 10 min.", "[Washing steps]\nRemove supernatant with a pipette.", "[Washing steps]\nResuspend cell suspension with Volvic spring water by repipetting or gentle vortexing.", "[Preparations prior to sample loading]\nPlace cleaned stainless steel... |
71,480 | CALCIUM STAINING PROTOCOL | 4 | dx.doi.org/10.17504/protocols.io.kxygx9zewg8j/v1 | https://www.protocols.io/view/calcium-staining-protocol-ch2yt8fw | Machlusil Husna, Kusworini Handono, Hidayat Sujuti, Aulanni'am, Ettie Rukmigarsari | TITLE: CALCIUM STAINING PROTOCOL
AUTHORS: Machlusil Husna, Kusworini Handono, Hidayat Sujuti, Aulanni'am, Ettie Rukmigarsari
[DESCRIPTION]
Neurodegeneration due to neurotoxicity is one of the phenomena in temporal lobe epilepsy. Experimentally, hippocampal excitotoxicity process can occur due to kainic acid exposure, ... | ["[Calcium assay kit] We use Fluo-4 assay kit (calcium ab 228555) that provides a homogenous fluorescense-based assay for detecting the intracellular calcium mobilization", "[Material supplied in kit] Fluo-4 AM 1 vial\n10x F127 Plus 1 bottle (10 mL)\nHHBS (Hank's Buffer with 20 mM Hepes) 1 bottle (100 mL)", "[Reagent P... |
43,591 | Cell Lysis, Two-Step Purification, and RNase Digestion | 4 | dx.doi.org/10.17504/protocols.io.bntfmejn | https://www.protocols.io/view/cell-lysis-two-step-purification-and-rnase-digesti-bntfmejn | Clémentine Delan-Forino, David Tollervey | TITLE: Cell Lysis, Two-Step Purification, and RNase Digestion
AUTHORS: Clémentine Delan-Forino, David Tollervey
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The RNA exosome complex functions in both the accurate processing and rapid degradation of many classes of RNA in eukaryotes and Archaea. Fu... | ["[RNase Digestion and Binding to Ni-NTA Resin]\nThe concentration of RNaceIT used to footprint (trim) RNAs on protein of interest is determined empirically. Ideally, the reads will be long enough to map uniquely (~17 nt) but short enough to give good resolution of the protein-binding site. We aim to generate an averag... |
59,699 | protocol Animal-Assisted Therapy | 1 | dx.doi.org/10.17504/protocols.io.14egn76ymv5d/v1 | https://www.protocols.io/view/protocol-animal-assisted-therapy-b6itrcen | Mohammad Tahan | TITLE: protocol Animal-Assisted Therapy
AUTHORS: Mohammad Tahan
[DESCRIPTION]
Human-animal interactions are considered as being valuable and beneficial for the psychological health. Recently Animal-Assisted Therapy (AAT) has been included for client-therapist interaction.
[STEPS]
SECTION: Animal-Assisted Therapy
1. ... | ["[Animal-Assisted Therapy] Session one\tIntroduction, briefing, primary assessing and introduction to the course", "[Animal-Assisted Therapy] Session two\tGiving an introduction about animals and their specific characteristics", "[Animal-Assisted Therapy] Session three\tTaking children to the place that animals were k... |
93,489 | Wire Hang Assessment | 4 | dx.doi.org/10.17504/protocols.io.3byl4qy9zvo5/v1 | https://www.protocols.io/view/wire-hang-assessment-c7irzkd6 | Tim Sampson, Ian N Krout, Alexandria White | TITLE: Wire Hang Assessment
AUTHORS: Tim Sampson, Ian N Krout, Alexandria White
[DESCRIPTION]
The wire hang test for mice is a simple and cheap way to asse motor and neuromotor pathologies in the mouse model. Described herein is an adapted protocol from Deacon 2013.
[STEPS]
SECTION: Apparatus
1. A wire hang apparatus... | ["[Apparatus] A wire hang apparatus can be made easily for use in this protocol and requires very little material (see Figure 1 for example). The apparatus should be easily traversed by the mice, meaning the spacing of the bars should be close enough to allow for free and easy movement of the mice and the width of the ... |
101,134 | NIBS using tDCS to mitigate aggression | 0 | dx.doi.org/10.17504/protocols.io.261ge5597g47/v1 | https://www.protocols.io/view/nibs-using-tdcs-to-mitigate-aggression-dezn3f5e | Paola Di Maio | TITLE: NIBS using tDCS to mitigate aggression
AUTHORS: Paola Di Maio
[DESCRIPTION]
Protocol Description: Non Invasive Neuromodulation procedure using tDCS (transcranial direct current stimulation) to mitigate aggressive behavior in healthy adults
Condition: tendency to exhibit overly aggressive behavior
Safety: The ... | ["[Safety Warnings] Use tDCS equipment and accessories compliant with international standards, by trained personnel", "In pre clinical studies, the procedure can mitigate Aggressive behaviour", "[Protocol Description] Two anodal electrodes are placed over the DLPFC bilaterally (F3 and F4) according to the International... |
42,301 | Cas9 Enrichment for Nanopore Sequencing | 1 | null | https://www.protocols.io/view/cas9-enrichment-for-nanopore-sequencing-bmi5k4g6 | Timothy Gilpatrick, Isac Lee, James E. Graham, Etienne Raimondeau, Rebecca Bowen, Andrew Heron, Fritz J. Sedlazeck, Winston Timp | TITLE: Cas9 Enrichment for Nanopore Sequencing
AUTHORS: Timothy Gilpatrick, Isac Lee, James E. Graham, Etienne Raimondeau, Rebecca Bowen, Andrew Heron, Fritz J. Sedlazeck, Winston Timp
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is designed to help users achieve targeted enrichment... | ["[Making Ribonucleoprotein Complex ]\nResuspend crRNA(s) and tracrRNA to final concentration of 100uM in TE, pH7.5(2nmol crRNA in 20uL; 5nmol tracrRNA in 50uL)", "[Making Ribonucleoprotein Complex ]\nMake equimolar mix of all crRNAs to be used by adding 0.75uL of each to new tube", "[Making Ribonucleoprotein Complex ]... |
null | null | null | dx.doi.org/10.17504/protocols.io.qncdvaw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This year´s interlab study allows participants to fulfil Bronze medal requirements. </p>
<p>For this purposes a set of experiments has to be performed, which in the end will be compared and validated with other team´s data. </p>
<p>Part of this Challenge are the Calibration p... | [] |
29,104 | UC Davis - Husbandry Care for Mice | null | dx.doi.org/10.17504/protocols.io.8nqhvdw | null | K.C. Kent Lloyd | TITLE: UC Davis - Husbandry Care for Mice
AUTHORS: K.C. Kent Lloyd
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">The purpose of this policy is to outline the minimum standards of care for rodents based on the Animal ... | ["[Daily Tasks]\nDaily Tasks – Refer to MBPVIV-20-105 Daily Observations/Action for further details Note: daily tasks must be performed every day 365 days a year 7 days a week including weekends and holidays and should be completed with appropriate PPE in place (minimum of laboratory coat and gloves)1.1 Provide nutriti... |
71,385 | Nanopore Rapid PCR Barcoding for Genomic Samples | 4 | null | https://www.protocols.io/view/nanopore-rapid-pcr-barcoding-for-genomic-samples-chxzt7p6 | Carlos Goller, Carly Sjogren | TITLE: Nanopore Rapid PCR Barcoding for Genomic Samples
AUTHORS: Carlos Goller, Carly Sjogren
[DESCRIPTION]
This protocol is for Rapid sequencing of DNA using PCR Barcoding (SQK-RPB004) and has been adapted from Oxford Nanopore Technologies.
[BEFORE_START]
Overall time is about 30 to 40 minutes.
COLLECT THESE:
Equi... | ["[Checklist] Gather Materials, Consumables and Equipment.\nThese are listed in the Materials tab also\n\nEquipment:\n-Microfuge\n-Timer\n-Thermal Cycler\n-Pipette and tips: P2, P10, P20, P100, P200, P1000\n\nConsumables:\n-1.5 ml Eppendorf DNA LoBind Tubes\n-0.2 ml thin-walled PCR tubes\n-Nuclease-free water\n-Agencou... |
63,385 | https://pillsfect.com/total-carbless-keto-gummies | 3 | dx.doi.org/10.17504/protocols.io.dm6gpb288lzp/v1 | https://www.protocols.io/view/https-pillsfect-com-total-carbless-keto-gummies-b95zr876 | H A | TITLE: https://pillsfect.com/total-carbless-keto-gummies
AUTHORS: H A
[DESCRIPTION]
you are presuming that because there is a potential benefit for them, the sales appointment is the next logical step.
[STEPS] | [] |
41,144 | Vagus Nerve Stimulation Evoked Electroneurography and Electromyography Recordings in Swine | 1 | dx.doi.org/10.17504/protocols.io.bkeyktfw | https://www.protocols.io/view/vagus-nerve-stimulation-evoked-electroneurography-bkeyktfw | Evan Nicolai, Kip A Ludwig | TITLE: Vagus Nerve Stimulation Evoked Electroneurography and Electromyography Recordings in Swine
AUTHORS: Evan Nicolai, Kip A Ludwig
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol was used to collect data now published in the Journal of Neural Engineering, Sources of off-target effec... | ["[Animal Preparation, Surgical Dissection, and Placement of Electrodes]\nWhat we did (approved by the Mayo Clinic IACUC):Weigh pig, and inject (I.M.) the pig with a mixture of telazol (6mg/kg), xylazine (2 mg/kg), and glycopyrrolate (0.006 mg/kg). Intubate the pig and anesthetize using isoflurane gas (1.5-3% in room a... |
89,254 | Preparation of tissue for Ribo-Tag/RNAseq analysis | 4 | dx.doi.org/10.17504/protocols.io.261gedwyyv47/v1 | https://www.protocols.io/view/preparation-of-tissue-for-ribo-tag-rnaseq-analysis-c3eeyjbe | Cecilia Tubert | TITLE: Preparation of tissue for Ribo-Tag/RNAseq analysis
AUTHORS: Cecilia Tubert
[DESCRIPTION]
This protocols describes the RiboTag virus vectors injected into mouse brain, tissue dissection, immunoprecipitation and RNA extraction for RNAseq.
[GUIDELINES]
Follow institutional guidelines and protocols.
[STEPS]
SEC... | ["[Stereotactic injection of RiboTag virus in the brain - Procedure] Prepare a clean empty mouse cage on a heating pad and a clean mouse cage with gel food for post-operatory care.", "[Stereotactic injection of RiboTag virus in the brain - Procedure] Set up sterile working area including stereotaxic frame", "[Stereotac... |
50,688 | Supporting protocol for use-case 2: Multi-modal 3D image reconstruction in "M2aia - Interactive, fast and memory efficient analysis of 2D and 3D multi-modal mass spectrometry imaging data" | 5 | dx.doi.org/10.17504/protocols.io.bvq8n5zw | https://www.protocols.io/view/supporting-protocol-for-use-case-2-multi-modal-3d-bvq8n5zw | Jonas Cordes | TITLE: Supporting protocol for use-case 2: Multi-modal 3D image reconstruction in "M2aia - Interactive, fast and memory efficient analysis of 2D and 3D multi-modal mass spectrometry imaging data"
AUTHORS: Jonas Cordes
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">We provide 10 consecutive brain sl... | ["[3D reconstruction]\nOpen the Preferences of M²aia, e.g. from the menu: Window > Preferences or Ctrl+P.Select M²aia's preference page and set the paths to the elastix and transformix executables, respectively.", "[3D reconstruction]\nOpen the Data view, e.g. from the menu: Window > Show View > DataOpen the Imaging t... |
45,838 | Brad-seq mRNA (for Shotgun or DGE) | 4 | null | https://www.protocols.io/view/brad-seq-mrna-for-shotgun-or-dge-bqznmx5e | Yin-Tse Huang | TITLE: Brad-seq mRNA (for Shotgun or DGE)
AUTHORS: Yin-Tse Huang
[DESCRIPTION]
Brad-seq mRNA
[STEPS]
SECTION: Tissue Lysis
1. Add 5 µL of 2-Mercaptoethanol (2-ME) to 1 mL of LBB (for ratio, adjust it for the amount LBB used)
SECTION: Tissue Lysis
2. Wipe off RNAlatter from tissue; Place 20 mgof tissue in crushing ... | ["[Tissue Lysis] Add 5 µL of 2-Mercaptoethanol (2-ME) to 1 mL of LBB (for ratio, adjust it for the amount LBB used)", "[Tissue Lysis] Wipe off RNAlatter from tissue; Place 20 mgof tissue in crushing tube with metal cone", "[Tissue Lysis] Add 200 µL in tube", "[Tissue Lysis] Crush sample with multi-beads shocker at 2000... |
60,810 | Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360 | 1 | dx.doi.org/10.17504/protocols.io.6qpvr6k7zvmk/v1 | https://www.protocols.io/view/mapping-cgrp-ir-innervation-of-male-mice-stomach-w-b7mirk4e | Duyen Nguyen, Jichao Ma | TITLE: Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360
AUTHORS: Duyen Nguyen, Jichao Ma
[DESCRIPTION]
This protocol describes the process of using Neurolucida 360 software to map the topographical organization of Calcitonin gene related peptide – immunoreactive axons and terminals in the muscu... | ["[Animals] Male C57BL/6J mice (The Jackson laboratory), n= 8, age 12-16 weeks were used in this study. Animals were kept in the animal room at which the dark/light cycle is set to 12/12 hours and water and food were supplied ad libitum. All procedures were carried out under the ethical guidelines of University of Cent... |
null | null | null | dx.doi.org/10.17504/protocols.io.cg7tzm | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
106,867 | Towards standardisation of parameter reporting for melt electrowriting | 0 | dx.doi.org/10.17504/protocols.io.4r3l2qzejl1y/v1 | https://www.protocols.io/view/towards-standardisation-of-parameter-reporting-for-dkkt4uwn | Sönke Menke, Biranche Tandon, Juergen Brugger | TITLE: Towards standardisation of parameter reporting for melt electrowriting
AUTHORS: Sönke Menke, Biranche Tandon, Juergen Brugger
[DESCRIPTION]
This protocol aims to help researchers collect all necessary data to make their research in melt electrowriting more reproducible and easier to compare to the data of other... | ["[Device information (Per device/configuration used)] If your system has been published before, make sure to add a reference to it, otherwise share a build guide or what modifications to published systems have been made as done by Eichholz et al. 2022 or Reizabal et al. 2023.", "[Device information (Per device/configu... |
43,594 | Generation of Library for Sequencing | 4 | dx.doi.org/10.17504/protocols.io.bntimeke | https://www.protocols.io/view/generation-of-library-for-sequencing-bntimeke | Clémentine Delan-Forino, David Tollervey | TITLE: Generation of Library for Sequencing
AUTHORS: Clémentine Delan-Forino, David Tollervey
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The RNA exosome complex functions in both the accurate processing and rapid degradation of many classes of RNA in eukaryotes and Archaea. Functional and struc... | ["[Reverse Transcription of Purified RNA]\nTo increase the efficiency of this step, prepare fresh dNTP dilution prior RT or aliquot and store at to avoid multiple thawing.", "[PCR Amplification of cDNA Libraries]\nThe number of cycles used to prepare cDNA libraries should be optimized for the template and limited to ... |
null | null | null | dx.doi.org/10.17504/protocols.io.rdud26w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The Seattle Children's Hospital launched a pioneering clinical trial of <a href="https://www.creative-biolabs.com/car-t/cellrapeutics-chimeric-antigen-receptor-car-technology.htm" target="_blank" rel="noopener noreferrer">chimeric antigen receptors</a> (CAR) T-cell immunother... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.m52c88e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This handout is based on the Caltech Reproducibility resource that was created by Code Ocean, Addgene, and protocols.io. It is extended to cover more tools and resources for biomedical scientists specifically.</p>
<p> </p>
<p>Please feel free to clone and modify it. If you do... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.r6gd9bw | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
39,907 | UH-PAM Protocol | 1 | dx.doi.org/10.17504/protocols.io.bi8bkhsn | https://www.protocols.io/view/uh-pam-protocol-bi8bkhsn | Seonggun Joe, Massimo Totaro, Hongbo Wang, Lucia Beccai | TITLE: UH-PAM Protocol
AUTHORS: Seonggun Joe, Massimo Totaro, Hongbo Wang, Lucia Beccai
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The protocol outlines the fabrication process of UH-PAM, and it prompts you with the details to repeat and reproduce our works.</div></div>
[STEPS]
?. Please downl... | ["Please download and unzip \"UH-PAM Protocol.zip\" in the attached file.", "Cut a commercial polyurethane open-cell foam and Plexigals as attached files (UH-PAM_2D Layers.dwg)We recommend 2D laser cutter (Versa LASER VLS 3.5), in order to properly cut them.For the print parameters, please follow the table, as below: ... |
70,124 | BICAN workflow protocol | 4 | null | https://www.protocols.io/view/bican-workflow-protocol-cgqktvuw | Satrajit Ghosh | TITLE: BICAN workflow protocol
AUTHORS: Satrajit Ghosh
[DESCRIPTION]
A test protocol.
[STEPS]
SECTION: Neuropixels workflow
1. Surgery
SECTION: Neuropixels workflow
1.1. Mouse head-fixed and anesthetized
SECTION: Neuropixels workflow
1.2. Headframe implant
SECTION: Neuropixels workflow
1.3. Craniotomy and durotomy... | ["[Neuropixels workflow] Surgery", "[Neuropixels workflow] Mouse head-fixed and anesthetized", "[Neuropixels workflow] Headframe implant", "[Neuropixels workflow] Craniotomy and durotomy", "[Neuropixels workflow] Cranial window implant", "[Neuropixels workflow] Photo documentation", "[Neuropixels workflow] Health and w... |
null | null | null | dx.doi.org/10.17504/protocols.io.vene3de | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
37,033 | My test protocol for a webinar | null | dx.doi.org/10.17504/protocols.io.bgehjtb6 | https://www.protocols.io/view/my-test-protocol-for-a-webinar-bgehjtb6 | Monica Hassan, Minnie Mouse | TITLE: My test protocol for a webinar
AUTHORS: Monica Hassan, Minnie Mouse
[STEPS]
?. Step 1 description
?. Step 2 description | ["Step 1 description", "Step 2 description"] |
43,968 | Variant functionalization by localization | 4 | dx.doi.org/10.17504/protocols.io.bn68mhhw | https://www.protocols.io/view/variant-functionalization-by-localization-bn68mhhw | jessechao | TITLE: Variant functionalization by localization
AUTHORS: jessechao
[STEPS]
?. [Cell culture and transfection]
Grow cells to sub confluent.For example, using MCF10A cells, seed cells at 2x105 cells per well in a 6-well plate 24h before transfection and incubate overnight.
?. [Cell culture and transfection]
Transfect... | ["[Cell culture and transfection]\nGrow cells to sub confluent.For example, using MCF10A cells, seed cells at 2x105 cells per well in a 6-well plate 24h before transfection and incubate overnight.", "[Cell culture and transfection]\nTransfect cells according to manufacturer's recommendations. For example, we used lipo... |
25,609 | Paired design of ATP bioluminescence method and colony counting method | null | dx.doi.org/10.17504/protocols.io.49hgz36 | null | Huiqiong XU; Jiansheng LIANG; Yimei WANG; Bin WANG; Tianbao ZHANG; Xiaoli LIU1; Lin GONG | TITLE: Paired design of ATP bioluminescence method and colony counting method
AUTHORS: Huiqiong XU; Jiansheng LIANG; Yimei WANG; Bin WANG; Tianbao ZHANG; Xiaoli LIU1; Lin GONG
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The ATP bioluminescence method can detect the ATP content in a sample by mon... | ["Prepare the standard curveDevice: B (BT-112D, Beijing Chuangxin Shiji Biochemical Science&Technology Development Co.,Ltd., China), P (SystemSURE Plus, Hygiena, USA), and N (Clean-Trace NGi, 3M, USA), pipetteReagent: ATP standard solution (The concentration of ATP standard solution should be no less than 1.0×10-7 mol/... |
18,350 | Using fluorescent beads to measure feeding rate in C. elegans | null | dx.doi.org/10.17504/protocols.io.v6ne9de | null | Emily Troemel | TITLE: Using fluorescent beads to measure feeding rate in C. elegans
AUTHORS: Emily Troemel
[STEPS] | [] |
45,767 | Generating Mutant Renal Cell Lines Using CRISPR Technologies | 4 | dx.doi.org/10.17504/protocols.io.bqxfmxjn | https://www.protocols.io/view/generating-mutant-renal-cell-lines-using-crispr-te-bqxfmxjn | Nuria Perretta-Tejedor, Grace Freke, Marian Seda, David. A. Long, Dagan Jenkins | TITLE: Generating Mutant Renal Cell Lines Using CRISPR Technologies
AUTHORS: Nuria Perretta-Tejedor, Grace Freke, Marian Seda, David. A. Long, Dagan Jenkins
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Gene editing using the CRISPR/Cas9 system is an extremely efficient approach for generating mut... | ["[3.1 sgRNA Design]\nFind your gene of interest within the relevant species on the Ensembl genome browser (www.ensembl.org). For HEK293 search the human genome, and for IMCD3 search the mouse genome.", "[3.1 sgRNA Design]\nSelect a 23–500-nucleotide (nt) genomic region that you wish to edit (see Note 3).", "[3.1 sgRNA... |
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