id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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67,380 | Sample preparation for aCGH karyotyping | 1 | dx.doi.org/10.17504/protocols.io.kxygxzdrov8j/v1 | https://www.protocols.io/view/sample-preparation-for-acgh-karyotyping-cd2us8ew | Hanqin Li, Dirk Hockemeyer | TITLE: Sample preparation for aCGH karyotyping
AUTHORS: Hanqin Li, Dirk Hockemeyer
[DESCRIPTION]
This protocol describes the standard procedure preparing cell pellets from human pluripotent stem cells (hPSCs) cultured on MEFs for outsourced aCGH karyotyping.
General Notes:
1. Throughout these protocols, the term hP... | ["[Collecting hPSCs colonies from MEFs] Use one, almost confluent, 6-well plate of hPSCs to prepare cell pellet", "[Collecting hPSCs colonies from MEFs] Wash once with DPBS", "[Collecting hPSCs colonies from MEFs] Add 1 ml of 1 mg/ml collagenase solution into each well to separate hPSC colonies from MEFs. Incubate 30 m... |
64,427 | Light sheet Sample Processing - Mouse Brain | 1 | dx.doi.org/10.17504/protocols.io.14egn7ykmv5d/v1 | https://www.protocols.io/view/light-sheet-sample-processing-mouse-brain-ca6jshcn | Nagham Khouri-Farah, James Li | TITLE: Light sheet Sample Processing - Mouse Brain
AUTHORS: Nagham Khouri-Farah, James Li
[DESCRIPTION]
An improved method for light-sheet imaging sample preparation. We use this protocol to process brain tissue (cerebellum) for light sheet imaging. First, we fix the tissue overnight at 4C, then we steam the tissue... | ["[REAGENT SETUP] PFA: 4% Paraformaldehyde. Can be stored at 4 °C to be used within 3-4 weeks.\nThe following solutions can be prepared in large volumes and stored in room temperature for months:\n1X Phosphate Buffered Saline (PBS)\n0.01 mM Citric Acid Buffer (pH 6)\nTEA: 0.1 M Triethanolamine in water\n10% SDS\nBA Bu... |
27,833 | HPC Account Set up and Access | null | dx.doi.org/10.17504/protocols.io.7ezhjf6 | null | Courtney Comrie | TITLE: HPC Account Set up and Access
AUTHORS: Courtney Comrie
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol will provide instruction on where to access the HPC lab account, and where our data is stored.</div><div class = "text-block">Only for internal use.</div></div>
[STEPS]
?. Go ... | ["Go to the main HPC information webpage, and select web interface under the Services section", "[Access to HPC ]\nEnter Dr. Hutchinson's email into the sponsor's email box so she can add you to the drive: hutchinsone@email.arizona.edu", "After this is done you must wait till accepted onto the lab account.", "[Access t... |
98,140 | YDV Multiplex PCR | 0 | dx.doi.org/10.17504/protocols.io.5qpvok5obl4o/v1 | https://www.protocols.io/view/ydv-multiplex-pcr-db342qqw | Stephen Byrne, Virgile Ballandras, Louise McNamara | TITLE: YDV Multiplex PCR
AUTHORS: Stephen Byrne, Virgile Ballandras, Louise McNamara
[DESCRIPTION]
This protocol describes the process of carrying out a multiplex PCR assay followed by capillary electrophoresis to detect C/BYDV and determine species (BYDV-MAV, BYDV-PAS, BYDV-PAV, CYDV (RPS or RPV)). The starting point... | ["[PCR protocol] Identify samples for analysis, including positive (virus infected aphid/barley) and negative controls (virus free aphid/barley), a positive control consisting of a mix of gBlocks with sequences for all targets in the multiplex PCR, and a no-template control. Details on the gBlocks can be found here:",... |
42,445 | DNA Extraction from FANS sorted nuclei | 4 | dx.doi.org/10.17504/protocols.io.bmpmk5k6 | https://www.protocols.io/view/dna-extraction-from-fans-sorted-nuclei-bmpmk5k6 | Stefania Policicchio, Jonathan P Davies, Barry Chioza, Aaron Jeffries, Joe Burrage, Jonathan Mill, Emma Dempster | TITLE: DNA Extraction from FANS sorted nuclei
AUTHORS: Stefania Policicchio, Jonathan P Davies, Barry Chioza, Aaron Jeffries, Joe Burrage, Jonathan Mill, Emma Dempster
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Here we describe an optimised protocol for the extraction of genomic DNA from fr... | ["[DNA extraction from FANS purified nuclei]\nDefrost frozen FANS sorted nuclei contained in a 1.5ml eppendorf tube\non ice\nNOTE 1-2 nuclei aliquots (~200,000 nuclei/tube) per each nuclei population should yield between 300-500ng DNA).", "[DNA extraction from FANS purified nuclei]\nTo each sample add either 250µL / 50... |
50,481 | Protocol for preparing minced Artemia nauplii | 2 | dx.doi.org/10.17504/protocols.io.bvirn4d6 | https://www.protocols.io/view/protocol-for-preparing-minced-artemia-nauplii-bvirn4d6 | Ranjith Lakshmanan, Linga Prabu Dhanasekaran, Kalidas C, Mathanbabu A, Saravanan Raju | TITLE: Protocol for preparing minced Artemia nauplii
AUTHORS: Ranjith Lakshmanan, Linga Prabu Dhanasekaran, Kalidas C, Mathanbabu A, Saravanan Raju
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Protocol for preparing minced </span><span style = "font-weight:bol... | [] |
80,062 | Total nucleic acid extraction - NucleoMag® DNA/RNA Water Kit (Macherey-Nagel) | 4 | dx.doi.org/10.17504/protocols.io.e6nvwj7xwlmk/v1 | https://www.protocols.io/view/total-nucleic-acid-extraction-nucleomag-dna-rna-wa-cse6wbhe | Adélaïde Roguet, Melissa Schussman | TITLE: Total nucleic acid extraction - NucleoMag® DNA/RNA Water Kit (Macherey-Nagel)
AUTHORS: Adélaïde Roguet, Melissa Schussman
[DESCRIPTION]
Total nucleic acid extraction from wastewater using NucleoMag® DNA/RNA Water Kit (Macherey-Nagel)
[BEFORE_START]
1. Clean the working area and all equipment: wipe down with 10... | ["[Total nucleic acid extraction] For HA filter extraction, let the sample thaw on ice and go to step 2. \n\nFor BCoV/BRSV extraction (in duplicate), add 5 µL of BCoV/BRSV solution to the 2-mL tube containing 500 µL MWA1. Vortex for 15 seconds (speed 7 out of 10) and flash freeze the tube. Go to step 4.\n\nFor Direct e... |
59,004 | TH Densitometry | 4 | dx.doi.org/10.17504/protocols.io.b5u4q6yw | https://www.protocols.io/view/th-densitometry-b5u4q6yw | Haley Geertsma | TITLE: TH Densitometry
AUTHORS: Haley Geertsma
[DESCRIPTION]
This protocol is used to quantify the density of dopaminergic projections to the striatum.
[STEPS]
1. Take 4 cryosections to equally sample the striatum and perform diaminobenzidine staining with a tyrosine hydroxylase antibody.
Note: I take every 12th se... | ["Take 4 cryosections to equally sample the striatum and perform diaminobenzidine staining with a tyrosine hydroxylase antibody.\nNote: I take every 12th section from Bregma 1.18mm to -0.22mm", "Capture brightfield images of the dorsolateral striatum and convert to greyscale images.", "Take 20 intensity measurements of... |
86,084 | Hot alkaline lysis gDNA extraction from formalin-fixed archival tissues | 4 | dx.doi.org/10.17504/protocols.io.yxmvm32p6l3p/v1 | https://www.protocols.io/view/hot-alkaline-lysis-gdna-extraction-from-formalin-f-cybcxsiw | Erin E Hahn, marina.alexander, Alicia Grealy, clare.holleley | TITLE: Hot alkaline lysis gDNA extraction from formalin-fixed archival tissues
AUTHORS: Erin E Hahn, marina.alexander, Alicia Grealy, clare.holleley
[DESCRIPTION]
The widespread practice of formalin preservation has historically limited genomic analysis of archival museum specimens.
Here we describe sample selection... | ["[Specimen vetting] Measure the pH of the media.", "[Specimen vetting] Visually assess the specimen. If the specimen appears decomposed, consider selecting an alternative specimen. Generally, specimens with discernible internal organs tend to have higher likelihood of success. If the internal organs have been removed,... |
40,288 | COVID Airway Processing for scRNAseq | 1 | dx.doi.org/10.17504/protocols.io.bjj8kkrw | https://www.protocols.io/view/covid-airway-processing-for-scrnaseq-bjj8kkrw | Peter Szabo, Steven Wells, Peter A. Sims, Donna Farber | TITLE: COVID Airway Processing for scRNAseq
AUTHORS: Peter Szabo, Steven Wells, Peter A. Sims, Donna Farber
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the isolation of lymphocytes and pan-mononuclear cells from airway samples.</div></div>
[STEPS]
?. [Preparing Buffer an... | ["[Preparing Buffer and Media]\nCreate the following DPBS Solution-EDTA in a bottle of DPBS by using the table below: ABCD1ComponentVolume (mL)Starting Conc.Final Conc.2DPBS474-3FBS25100%5%4EDTA10.5 M1 mM\nABCD1ComponentVolume (mL)Starting Conc.Final Conc.2DPBS474-3FBS25100%5%4EDTA10.5 M1 mM", "[Preparing Buffer and M... |
22,948 | Pig Nodose Ganglion protocol | null | dx.doi.org/10.17504/protocols.io.2ncgdaw | null | Marmar Vaseghi, Jeffrey Ardell | TITLE: Pig Nodose Ganglion protocol
AUTHORS: Marmar Vaseghi, Jeffrey Ardell
[STEPS]
?. Animal: Yorkshire Pig
?. Surgical Prep: Sedation Telazol (8mg/kg)Anesthesia isoflurane (1-2% inhalation) concomitant with intermittent boluses of fentanyl (1–3 μg/kg iv)
?. Vascular access: femoral artery (pressure) veins (fluid)mid... | ["Animal: Yorkshire Pig", "Surgical Prep: Sedation Telazol (8mg/kg)Anesthesia isoflurane (1-2% inhalation) concomitant with intermittent boluses of fentanyl (1–3 μg/kg iv)", "Vascular access: femoral artery (pressure) veins (fluid)mid-sternotomy: access to heart, great vessels", "Nodose- Neuronal output recording of sp... |
93,748 | Current Educational Practice in Physiotherapy Simulation-Based Education: A Scoping Review Protocol | 1 | dx.doi.org/10.17504/protocols.io.bp2l6xww1lqe/v1 | https://www.protocols.io/view/current-educational-practice-in-physiotherapy-simu-c7suznew | Yasir Qaiser Choudhary, Orlagh O’Shea, Helen P French, Claire Condron, Helen Heery, Suzanne McDonough, Walter Eppich | TITLE: Current Educational Practice in Physiotherapy Simulation-Based Education: A Scoping Review Protocol
AUTHORS: Yasir Qaiser Choudhary, Orlagh O’Shea, Helen P French, Claire Condron, Helen Heery, Suzanne McDonough, Walter Eppich
[DESCRIPTION]
Background:
Simulation-Based Education (SBE) is an educational techniqu... | ["[Introduction] Simulation is an educational technique used to substitute and augment real experiences with guided ones that are frequently ‘’immersive’’ in nature and that accurately evoke or replicate significant features of the real environment in a fully interactive way (Gaba, 2004; Harder, 2010). Simulation in ph... |
16,775 | CUT&RUN with Drosophila tissues | null | dx.doi.org/10.17504/protocols.io.umfeu3n | null | Kami Ahmad | TITLE: CUT&RUN with Drosophila tissues
AUTHORS: Kami Ahmad
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">We have modified the Cleavage Under Targets and Release Using Nuclease (CUT&RUN) method for epigenomic profiling of histone modifications and chromatin proteins to use dissected Drosophila tiss... | ["[Prepare solutions and beads]\nPrepare a fresh 5% digitonin solution as follows:Weigh out 50 mg digitonin powder in a 2 ml microcentrifuge tube. Boil some water in a small beaker in a microwave oven, and pipette in and out to warm the 1000 μL pipette tip. Pipette the hot water into the tube with the digitonin powder,... |
28,769 | Measles virus TaqMan RT-PCR (F gene; no longer in regular use; see Guidelines) | null | dx.doi.org/10.17504/protocols.io.8b9hsr6 | null | Mitchell Finger, Michael Lyon, Judy Northill, Ian Mackay | TITLE: Measles virus TaqMan RT-PCR (F gene; no longer in regular use; see Guidelines)
AUTHORS: Mitchell Finger, Michael Lyon, Judy Northill, Ian Mackay
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><ul style = "list-style-type:disc;"><li style = "counter-reset:ol0;list-style-type:disc;">This real-... | ["[Oligonucleotide sequences]\nAB1NameSequence 5'-3'2Primer Measles MGB FPGCTCAAATTGCTCAGATACTATACAGAAA3Primer Measles MGB RPGCAGATATGGGGTCCCGTAA\n4Probe Measles MGB ProbeFAM - CCTGTCATTATTTGGCC - MGBNFQ\nAB1NameSequence 5'-3'2Primer Measles MGB FPGCTCAAATTGCTCAGATACTATACAGAAA3Primer Measles MGB RPGCAGATATGGGGTCCCGTAA\... |
78,536 | Data analysis code for bioinformatics and statistics | 1 | dx.doi.org/10.17504/protocols.io.yxmvm2b99g3p/v1 | https://www.protocols.io/view/data-analysis-code-for-bioinformatics-and-statisti-cqxgvxjw | null | TITLE: Data analysis code for bioinformatics and statistics
AUTHORS:
[DESCRIPTION]
The protocol details the data analysis code for bioinformatics and statistics.
[STEPS] | [] |
61,846 | Tube Radius Calculation | 1 | dx.doi.org/10.17504/protocols.io.x54v9y3rqg3e/v1 | https://www.protocols.io/view/tube-radius-calculation-b8mwru7e | Liv Jensen | TITLE: Tube Radius Calculation
AUTHORS: Liv Jensen
[DESCRIPTION]
This protocol details Tube Radius Calculation.
[STEPS]
SECTION: Fluorescence ratio calibration:
1. Measure the radius of the GUV and of the aspiration pipette from imaging data.
SECTION: Fluorescence ratio calibration:
2. Calculate radius from:
S... | ["[Fluorescence ratio calibration:] Measure the radius of the GUV and of the aspiration pipette from imaging data.", "[Fluorescence ratio calibration:] Calculate radius from:", "[Fluorescence ratio calibration:] In FIJI, create new image stacks from two rectangular selections: one containing a section of the membrane t... |
30,352 | Syngenta drug screen | null | dx.doi.org/10.17504/protocols.io.9vqh65w | null | Ida Barlow, Adam McDermott-Rouse, Luigi Feriani | TITLE: Syngenta drug screen
AUTHORS: Ida Barlow, Adam McDermott-Rouse, Luigi Feriani
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for preparing worms, preparing drug plates, dispensing worms with wormsorter and tracking on Hydra rigs</div></div>
[STEPS]
?. [Bleach worms (- 5 days)]
Blea... | ["[Bleach worms (- 5 days)]\nBleach worms prepared for day 1 tracking:Adaptations:- remix pellet after each wash\n{\"blocks\":[{\"key\":\"aisuj\",\"text\":\"This protocol is for obtaining synchronised populations of C. elegans. Eggs are harvested from gravid hermaphrodites and allow to grow to L1 arrest for 12-120 hour... |
95,194 | Purification of recombinant Tau Repeat Domain (TauRD) from Escherichia coli | 1 | dx.doi.org/10.17504/protocols.io.x54v9p6p1g3e/v1 | https://www.protocols.io/view/purification-of-recombinant-tau-repeat-domain-taur-c872zzqe | Patricia Yuste-Checa, F Ulrich Hartl | TITLE: Purification of recombinant Tau Repeat Domain (TauRD) from Escherichia coli
AUTHORS: Patricia Yuste-Checa, F Ulrich Hartl
[DESCRIPTION]
This protocol details how to efficiently purify the recombinant Tau repeat domain from Escherichia coli.
[GUIDELINES]
NOTE : This protocol was optimized for purification of th... | ["[TauRD expression] Thaw RbCl-competent Escherichia coli Bl21 cells (DE3) on ice.", "[TauRD expression] Add 1 µL of pHUE-TauRD plasmid (His 6 -ubiquitin-TauRD) and incubate 30 min on ice.", "[TauRD expression] Heat shock 45 s at 42 °C.", "[TauRD expression] Incubate on ice 2 min, then add 850 µL Lysogeny broth (LB) or... |
33,934 | Human embryonic stem cells differentiation into oligodendrocyte lineage cells | null | dx.doi.org/10.17504/protocols.io.bddni25e | null | Paria Pooyan | TITLE: Human embryonic stem cells differentiation into oligodendrocyte lineage cells
AUTHORS: Paria Pooyan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Oligodendrocyte (OL) lineage cell generation from human embryonic stem cell line Royan H6 (RH6) started by dual inhibition of SMAD signalin... | ["6 ×106 RH6 cells were plated on Engelbreth-Holm-Swarm mouse sarcoma ECM (sigma) coated 6cm cell culture plates on day -1.", "On day 0, the FFC medium (containing 20% KSR and 100 ng/ml bFGF) was replaced by 4 ml NI medium. NI medium was refreshed every day.", "From day 4, 5% KSR was gradually withdrawn from the NI med... |
null | null | null | dx.doi.org/10.17504/protocols.io.smgec3w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>To comprehensively investigate the effects of different diets on fish muscle quality and metabolic alterations, a large-scale metabolomics study was performed.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.k9ncz5e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Intended use:</strong></p>
<p>Kinetic system for Alanine Aminotransferase (ALT/ GPT) activity determination.</p>
<p> </p>
<p><strong>Teste Principle:</strong></p>
<p> </p>
<p><strong>ALT</strong> catalyzes the transfer of amino groups from Alanine to Ketoglutarate, yi... | [] |
36,085 | Drug Behaviour Imaging on Phenix | null | dx.doi.org/10.17504/protocols.io.bfgvjjw6 | https://www.protocols.io/view/drug-behaviour-imaging-on-phenix-bfgvjjw6 | Ida Barlow, Adam Mcdermott-Rouse | TITLE: Drug Behaviour Imaging on Phenix
AUTHORS: Ida Barlow, Adam Mcdermott-Rouse
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">For imaging drug-treated young adult C. elegans in liquids using the Multiworm tracker. Worms are synchronised by picking L4s, and then the young adults are exposed to dr... | ["[Making up the drugs (-1 Days)]\nFirst identify the drugs to be used in the study and ensure that they are correctly labelled and handled.", "[Making up the drugs (-1 Days)]\nCalculate the desired weight and volume of solvent required for the desired concentration if using powdered compounds. Alternatively calculate ... |
null | null | null | dx.doi.org/10.17504/protocols.io.gzzbx76 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
45,549 | 1.1 Bead beating | 4 | null | https://www.protocols.io/view/1-1-bead-beating-bqqmmvu6 | Elizabeth Fozo | TITLE: 1.1 Bead beating
AUTHORS: Elizabeth Fozo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Sample preparation for Western bolt</div></div>
[STEPS]
?. [Sample preparation for Western blot]
Bead beating
?. [Sample preparation for Western blot]
Bead size selection0.1 mm diameter for bacteria, 0.5... | ["[Sample preparation for Western blot]\nBead beating", "[Sample preparation for Western blot]\nBead size selection0.1 mm diameter for bacteria, 0.5 mm diameter for yeast, and 1 or 2.5 mm diameter for a chopped-up plant or animal tissue.Fill2 ml screw-cap microtube with0.5 gm of beads, keep the tubes in ice and add cel... |
48,530 | Modular generation of cortical, striatal and ventral midbrain progenitor cells | 4 | dx.doi.org/10.17504/protocols.io.btmsnk6e | https://www.protocols.io/view/modular-generation-of-cortical-striatal-and-ventra-btmsnk6e | Carles Calatayud, Esther Muñoz-Pedrazo, Sandra Fernández-Gallego, Patrik Verstreken | TITLE: Modular generation of cortical, striatal and ventral midbrain progenitor cells
AUTHORS: Carles Calatayud, Esther Muñoz-Pedrazo, Sandra Fernández-Gallego, Patrik Verstreken
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The present protocol describes the modular generation of cortical, st... | ["[Preparation of the hiPSC for starting the neuralization]\nNotes before starting:We maintain hPSC in mTESR-Plus medium on Matrigel-coated plates and split the cells as clumps using RELESR or 0.5 mM EDTA. Accutase is used when cells need to be counted such as in the initial step of the protocol. Or for the first passa... |
null | null | null | dx.doi.org/10.17504/protocols.io.gcqbsvw | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
<p><strong>Aseptic Removal of Femurs</strong></p>
<p> </p>
<p>Small beaker of 70% EtOH</p>
<p>Scissors</p>
<p>Dissection blades</p>
<p>Tweezers</p>
<p>Ice</p>
<p>50 mL Falcon with 25 mL cold phenol red free media or PBS on ice for each strain used.</p>
<p>Dissection board and p... | [] |
69,118 | Enhanced QIAseq DIRECT SARS-CoV-2 Kit for Illumina MiSeq | 4 | dx.doi.org/10.17504/protocols.io.rm7vzy39rlx1/v4 | https://www.protocols.io/view/enhanced-qiaseq-direct-sars-cov-2-kit-for-illumina-cfq6tmze | Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim | TITLE: Enhanced QIAseq DIRECT SARS-CoV-2 Kit for Illumina MiSeq
AUTHORS: Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim
[DESCRIPTION]
This SARS-CoV-2 targeted, a rapid viral RNA library preparation kit compatible with Illumina sequencers, provides an approximately five hour turn-aro... | ["[cDNA Synthesis Procedure]", "[cDNA Synthesis Procedure] Thaw template RNA on ice. Gently yet thoroughly mix, then briefly centrifuge to collect residual liquid from the sides of the tubes and return to ice.", "[cDNA Synthesis Procedure] While keeping reagents on ice, prepare the cDNA reaction according to the table ... |
98,446 | CODA (part 3): deep learning tissue structures labeling | HuBMAP | JHU-TMC | 0 | dx.doi.org/10.17504/protocols.io.81wgbz1x3gpk/v2 | https://www.protocols.io/view/coda-part-3-deep-learning-tissue-structures-labeli-dcdn2s5e | Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz | TITLE: CODA (part 3): deep learning tissue structures labeling | HuBMAP | JHU-TMC
AUTHORS: Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz
[DESCRIPTION]
In this section, we describe steps to create a basic semantic segmentation algorithm using CODA. CODA uses a modified resnet50 net... | ["[Deep learning multi-labelling of tissue structures using training on manual annotations] Choose the biological structures you wish to segment in your images. Semantic segmentation algorithms must classify every pixel of every image with a label, so your list must be exhaustive. For example, in lung histology you cou... |
88,581 | Ovarian Tissue Procurement from Organ Donor | 1 | dx.doi.org/10.17504/protocols.io.81wgbxn83lpk/v1 | https://www.protocols.io/view/ovarian-tissue-procurement-from-organ-donor-c2rdyd26 | pooja.devrukhkar, hannah.anvari, asma.giornazi Asma Giornazi, shriya.shah, maryellen.pavone, francesca.e.duncan francesca.duncan | TITLE: Ovarian Tissue Procurement from Organ Donor
AUTHORS: pooja.devrukhkar, hannah.anvari, asma.giornazi Asma Giornazi, shriya.shah, maryellen.pavone, francesca.e.duncan francesca.duncan
[DESCRIPTION]
The purpose of this protocol is to describe the procurement of human ovarian tissue from organ donors for research p... | ["[Collection of ovarian tissue for research - Done by Pathology] The NU-RTL is an established IRB-approved central repository that allows the collection of clinical specimens from participants including but not limited to gynecologic tissue, follicular fluid, and associated bio-fluids. This material is obtained at th... |
29,257 | Copy of Fluorescent in vitro model to assess adhesion of Bd to A6 cells (Plos One) | null | dx.doi.org/10.17504/protocols.io.8thhwj6 | null | Elin Verbrugghe | TITLE: Copy of Fluorescent in vitro model to assess adhesion of Bd to A6 cells (Plos One)
AUTHORS: Elin Verbrugghe
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The largest current disease-induced loss of vertebrate biodiversity is due to chytridiomycosis and despite the increasing understan... | ["Prepare Cell Medium A: L15 medium: 70%Distilled water: 20%Fetal bovine serum 10%", "Prepare Cell Medium B: L15 medium: 40%Distilled water: 55%Fetal bovine serum: 5%", "Coat coverslips with Rat tail collagen: Add glass coverslips in a 24-well tissue culture plate. Coat the glass coverslips at 37°C for 2 hours. Therefo... |
57,642 | Medium without CRF for Neocallimastigomycota | 4 | dx.doi.org/10.17504/protocols.io.36wgq779kvk5/v1 | https://www.protocols.io/view/medium-without-crf-for-neocallimastigomycota-b4iiquce | Julia Vinzelj, Diana Young, Akshay Joshi, Sophia Strobl, Ljubica Begovic, Nico Peer, Magdalena Nagler, Rolf Warthmann, Veronika Flad, Urs Baier, Michael Lebuhn, Sabine Podmirseg | TITLE: Medium without CRF for Neocallimastigomycota
AUTHORS: Julia Vinzelj, Diana Young, Akshay Joshi, Sophia Strobl, Ljubica Begovic, Nico Peer, Magdalena Nagler, Rolf Warthmann, Veronika Flad, Urs Baier, Michael Lebuhn, Sabine Podmirseg
[DESCRIPTION]
This is the protocol for the standard, nutrient-rich, undefined cu... | ["Add Salt Solution I, Salt Solution II, Yeast Extract, Tryptone, Hemin Solution, Resazurin Solution, Xylan powder (as an additional C-source for AF; can be omitted), and MilliQ water to a cooking pot.", "Heat the mixture and simmer it until the colour changes to dark yellow. For us with 2 L of medium in a 5 L cooking ... |
null | null | null | dx.doi.org/10.17504/protocols.io.twbepan | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
Prepare 4mg/mL collagenase solution before start and place on 37C shaker to dissolve.
[STEPS]
SECTION: Prepare materials
?.
SECTION: Initial Tissue Preperation
?.
SECTION: Mechanical Dissociation of Tissue
?.
SECTION: Enzymatic Dissociation of Tissue
?.
SECTION: Organoid C... | ["[Prepare materials] Collagenase Solution\n100mg/mL Collagenase Type I in DMEM/Ham's F12\n\nMedia\nDMEM/Ham's F12\n10% FBS\n1% Antibiotic\n\nDMEM/Ham's F12\n5% FBS\n1% Antibiotic\n\nDNAse\n1mg/mL DNase in PBS", "[Initial Tissue Preperation] Transfer tissues to 150cm plate.\nWash with large quantities of PBS to remove ... |
58,130 | DToL Taxon-specific Standard Operating Procedure for the Terrestrial and Freshwater Arthropods Working Group | 2 | dx.doi.org/10.17504/protocols.io.261gennyog47/v1 | https://www.protocols.io/view/dtol-taxon-specific-standard-operating-procedure-f-b4zsqx6e | Lyndall Pereira, Olga Sivell, Laura Sivess, Chris Fletcher, Gavin R. Broad, Liam Crowley, Inez Januszczak | TITLE: DToL Taxon-specific Standard Operating Procedure for the Terrestrial and Freshwater Arthropods Working Group
AUTHORS: Lyndall Pereira, Olga Sivell, Laura Sivess, Chris Fletcher, Gavin R. Broad, Liam Crowley, Inez Januszczak
[DESCRIPTION]
This Standard Operating Procedure (SOP) collection contains guidance on h... | [] |
41,023 | This is a test protocol | 1 | null | https://www.protocols.io/view/this-is-a-test-protocol-bka7kshn | Anita Broellochs | TITLE: This is a test protocol
AUTHORS: Anita Broellochs
[STEPS]
?. Test step | ["Test step"] |
53,107 | NCBI data curation protocol - SOP for editing GenomeTrakr submissions | 1 | dx.doi.org/10.17504/protocols.io.bx4tpqwn | https://www.protocols.io/view/ncbi-data-curation-protocol-sop-for-editing-genome-bx4tpqwn | Ruth Timme, Candace.Bias , Sai Laxmi Gubbala Venkata, Robyn Randolph, William Wolfgang, Errol Strain, Maria Balkey | TITLE: NCBI data curation protocol - SOP for editing GenomeTrakr submissions
AUTHORS: Ruth Timme, Candace.Bias , Sai Laxmi Gubbala Venkata, Robyn Randolph, William Wolfgang, Errol Strain, Maria Balkey
[DESCRIPTION]
PURPOSE: After data are submitted to NCBI submitters often encounter the need to update, retract,... | ["[BioProject Curation] How to edit a BioProject", "[BioProject Curation] Email for BioProject: bioprojecthelp@ncbi.nlm.nih.gov\n\nUse this email for the following tasks, include the BioProject accession in the email subject:\n\nQuestions about errors or processing of a BioProject submission\n\nConvert a Data BioProjec... |
80,508 | Labeling Protocol for Microarray Analysis | 4 | dx.doi.org/10.17504/protocols.io.kxygx9bxog8j/v1 | https://www.protocols.io/view/labeling-protocol-for-microarray-analysis-csu4weyw | creative-biogene | TITLE: Labeling Protocol for Microarray Analysis
AUTHORS: creative-biogene
[DESCRIPTION]
Since an array can contain tens of thousands of probes, a microarray experiment can accomplish many genetic tests in parallel. Therefore, arrays have dramatically accelerated many types of investigation. In standard microarrays, t... | ["[Experiment Summary] Since an array can contain tens of thousands of probes, a microarray experiment can accomplish many genetic tests in parallel. Therefore, arrays have dramatically accelerated many types of investigation. In standard microarrays, the probes are synthesized and then attached via surface engineering... |
null | null | null | dx.doi.org/10.17504/protocols.io.hnrb5d6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A sequence-independent method to turn genomic DNA into libraries of hairpins. </p>
<p> </p>
<p>An efficient way to get a different adapter on each end of a DNA fragment is to clone it and digest the fragment back out. Design the vector to have unique restriction sites outside... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.iktccwn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
108,373 | FLAM-seq (with Kapa mRNA enrichment)_CMS_edit-2024-09-25 | 0 | dx.doi.org/10.17504/protocols.io.j8nlk8q46l5r/v1 | https://www.protocols.io/view/flam-seq-with-kapa-mrna-enrichment-cms-edit-2024-0-dm3v48n6 | Corneliu Sologon | TITLE: FLAM-seq (with Kapa mRNA enrichment)_CMS_edit-2024-09-25
AUTHORS: Corneliu Sologon
[DESCRIPTION]
fork__CMS_edit-2024-09-25
[STEPS]
SECTION: GI Tailing (using USB poly(A) tail length assay, Thermo Fisher).
18. Prepare on ice (20 uL total):
14 µL
4 µL
2 µL
SECTION: GI Tailing (using USB poly(A) tail length as... | ["[GI Tailing (using USB poly(A) tail length assay, Thermo Fisher).] Prepare on ice (20 uL total):\n14 µL \n4 µL \n2 µL", "[GI Tailing (using USB poly(A) tail length assay, Thermo Fisher).] 1.5 µL \n4 °C 2 min", "[GI Tailing (using USB poly(A) tail length assay, Thermo Fisher).] 38.7 µL \n4 Room temperature 5 min", "[G... |
93,038 | DIY Intervention Testing in Daphnia: A simplified way to test lifespan interventions at home | 1 | null | https://www.protocols.io/view/diy-intervention-testing-in-daphnia-a-simplified-w-c64nzgve | Cora Anderson, Benyamin Matei-Dediu, Edouard Debonneuil, Rachael A Jonas-Closs, Leonid Peshkin | TITLE: DIY Intervention Testing in Daphnia: A simplified way to test lifespan interventions at home
AUTHORS: Cora Anderson, Benyamin Matei-Dediu, Edouard Debonneuil, Rachael A Jonas-Closs, Leonid Peshkin
[DESCRIPTION]
This guide is for amateur science enthusiasts who are looking to conduct safe and easy animal experim... | ["[Grandmother culture generation, your Daphnia reserve] First steps", "[Grandmother culture generation, your Daphnia reserve] Once the water begins to turn, green Daphnia may be added.", "[Grandmother culture generation, your Daphnia reserve] Once the grandmother culture is established and stabilized the only regular ... |
100,327 | Immunohistochemical labelling of spinal cord sections for chemoarchitectural analysis of segments | 1 | dx.doi.org/10.17504/protocols.io.14egn6376l5d/v1 | https://www.protocols.io/view/immunohistochemical-labelling-of-spinal-cord-secti-dd8f29tn | John-Paul Fuller-Jackson, Peregrine B Osborne, Janet R Keast | TITLE: Immunohistochemical labelling of spinal cord sections for chemoarchitectural analysis of segments
AUTHORS: John-Paul Fuller-Jackson, Peregrine B Osborne, Janet R Keast
[DESCRIPTION]
This protocol is used for immunohistochemical visualisation of the chemoarchitecture of the adult rat lumbosacral spinal cord (and... | ["[Preparation of cryosections] Cryoprotect fixed spinal cord segments in PBS containing 30% sucrose. This should be performed at 4 ºC, 24-72h prior to cutting.", "[Preparation of cryosections] Embed tissue in cryomold using OCT, freeze in cryostat and cut sections (40 µm), collecting sections progressively across sets... |
47,250 | Donor Acceptance Criteria for TMC Florida/Zurich HuBMAP Inclusion | 1 | dx.doi.org/10.17504/protocols.io.bsdsna6e | https://www.protocols.io/view/donor-acceptance-criteria-for-tmc-florida-zurich-h-bsdsna6e | Marda Jorgensen, Clive Wasserfall | TITLE: Donor Acceptance Criteria for TMC Florida/Zurich HuBMAP Inclusion
AUTHORS: Marda Jorgensen, Clive Wasserfall
[DESCRIPTION]
This document outlines the required criteria for inclusion for donors of thymus, lymph nodes and spleen in the HuBMAP project.
[STEPS]
SECTION: Donor Criteria
1. A copy of the complete ... | ["[Donor Criteria] A copy of the complete recovery checklist must be provided", "[Donor Criteria] BMI must be in keeping with CDC published growth charts 5th to 95th percentile", "[Donor Criteria] Donor must be less than seven days on ventilator", "[Donor Criteria] Donor tissue must be received within 24 hours of recov... |
45,131 | Standard Operating Procedure: Microbank | 3 | dx.doi.org/10.17504/protocols.io.bqbjmskn | https://www.protocols.io/view/standard-operating-procedure-microbank-bqbjmskn | Romina Gazis-Seregina, Pedro Pablo Parra | TITLE: Standard Operating Procedure: Microbank
AUTHORS: Romina Gazis-Seregina, Pedro Pablo Parra
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to store and retrieve bacterial and fungal cultures with Microbank™.</div><div class = "text-block"><span>This protocol is part... | [] |
45,450 | test protocol for test | 1 | null | https://www.protocols.io/view/test-protocol-for-test-bqmimu4e | Maria Guliakina | TITLE: test protocol for test
AUTHORS: Maria Guliakina
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">test</div></div>
[STEPS]
?. test
?. test 2 | ["test", "test 2"] |
43,344 | Coral and seawater 15N2 incubations | 1 | null | https://www.protocols.io/view/coral-and-seawater-15n2-incubations-bnjqmcmw | Molly Moynihan, Phyllis Yy Kho | TITLE: Coral and seawater 15N2 incubations
AUTHORS: Molly Moynihan, Phyllis Yy Kho
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Coral and seawater 15N2 incubations</div></div>
[STEPS]
?. [Pre-incubation preparation]
Fragment coral colonies in duplicate for control and experimental treatments. At... | ["[Pre-incubation preparation]\nFragment coral colonies in duplicate for control and experimental treatments. Attach to underwater rig to acclimate for 1 week prior to incubation.", "[Pre-incubation preparation]\nCollect seawater to filter-sterilize for incubation (can be done on day of fragmentation). Collect seawater... |
null | null | null | dx.doi.org/10.17504/protocols.io.rr6d59e | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
59,616 | Label-free quantification (LFQ) proteomic data analysis from DIA-NN output files | 5 | dx.doi.org/10.17504/protocols.io.5qpvobk7xl4o/v1 | https://www.protocols.io/view/label-free-quantification-lfq-proteomic-data-analy-b6f8rbrw | Yan Chen, Christopher J Petzold | TITLE: Label-free quantification (LFQ) proteomic data analysis from DIA-NN output files
AUTHORS: Yan Chen, Christopher J Petzold
[DESCRIPTION]
This protocol details the analysis of label-free quantification (LFQ) data from data independent acquisition (DIA) discovery (shotgun) proteomic experiments and generates a se... | ["[Data processing] We start with a DIA data acquisition peptide search output file the DIANN search (DIA; link to DIA-NN paper) and we trim out unused columns in the reports to simplify the analysis.\n\nDIA-NN report restricted to:\nProtein.Group\nProtein.Name\nGenes\nProtein.Description\nSample\nReplicate\nAbundance ... |
84,955 | Cleaning Protocol for Running Bacterial Samples on CytoFLEX S in Tube Mode | 1 | dx.doi.org/10.17504/protocols.io.q26g7p83kgwz/v1 | https://www.protocols.click/view/cleaning-protocol-for-running-bacterial-samples-on-cw73xhqn | Jamie C Tijerina | TITLE: Cleaning Protocol for Running Bacterial Samples on CytoFLEX S in Tube Mode
AUTHORS: Jamie C Tijerina
[DESCRIPTION]
The CytoFLEX S analyzer, like most other flow cytometers and cell sorters, was designed to run primarily mammalian cells. The acceptable threshold for carryover generally is <1% and this can be ach... | ["[Between Each Experimental Sample] You are now ready to run your next sample.", "[Between Each Experimental Sample] Manually backflush 2 times (click the backflush button and let it go through a 3-second cycle; do this 2x)", "[Between Each Experimental Sample] Change the flow rate to “Custom” using the radio button a... |
null | null | null | dx.doi.org/10.17504/protocols.io.jtpcnmn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes antigen stimulation of human peripheral blood mononuclear cells (PBMC), staining for various surface and intracellular molecular markers, followed by flow cytometry measurements. PBMC contain various T and NK cell populations, which produce cytokines u... | [] |
44,465 | Reconstituting LRRK2RCKW on Microtubules for cryo-EM studies | 4 | dx.doi.org/10.17504/protocols.io.bpnrmmd6 | https://www.protocols.io/view/reconstituting-lrrk2rckw-on-microtubules-for-cryo-bpnrmmd6 | Mariusz Matyszewski | TITLE: Reconstituting LRRK2RCKW on Microtubules for cryo-EM studies
AUTHORS: Mariusz Matyszewski
[DESCRIPTION]
This protocol contains a short instruction for reconstituting LRRK2RCKW on microtubules for cryo-EM studies with or without kinase inhibitors present.
[BEFORE_START]
Please take notice of the buffer preparat... | ["[Instruction] Add purified LRRK2RCKW and unpolymerized bovine tubulin dimer in a 2:1 molar ratio into the polymerization buffer (2 LRRK2RCKWs for each tubulin dimer). (Recommended total size: 10 µL)", "[Instruction] Allow the mixture to polymerize at Room temperature for at least 60 min.", "[Instruction] Prepare cryo... |
94,351 | Biotinylation by antibody recognition | 1 | dx.doi.org/10.17504/protocols.io.14egn3qq6l5d/v1 | https://www.protocols.io/view/biotinylation-by-antibody-recognition-c8dpzs5n | Bryan_Killinger | TITLE: Biotinylation by antibody recognition
AUTHORS: Bryan_Killinger
[DESCRIPTION]
This protocol details the biotinylation by antibody recognition.
[STEPS]
SECTION: Biotinylation by antibody recognition
1. Collect the brain sections at 240-micron intervals across the neuroaxis, place them into a net well (Brain rese... | ["[Biotinylation by antibody recognition] Collect the brain sections at 240-micron intervals across the neuroaxis, place them into a net well (Brain research laboratories) and wash 3 times for 60 min each in TBST.", "[Biotinylation by antibody recognition] Place the sections in 0.3% hydrogen peroxide and 0.1% sodium az... |
63,001 | Weight Crasher Keto Gummies - World #1 Weight Loss Supplement | 3 | dx.doi.org/10.17504/protocols.io.j8nlkk6d5l5r/v1 | https://www.protocols.io/view/weight-crasher-keto-gummies-world-1-weight-loss-su-b9rzr576 | H Douglas Morris | TITLE: Weight Crasher Keto Gummies - World #1 Weight Loss Supplement
AUTHORS: H Douglas Morris
[DESCRIPTION]
Tap the standard to guarantee your most memorable container! Do it today, and you can get the best Weight Crasher Keto Gummies Price that is at any point been offered!
[STEPS] | [] |
104,789 | Cas9 Protein Purification Protocol | 0 | dx.doi.org/10.17504/protocols.io.3byl49rpjgo5/v1 | https://www.protocols.io/view/cas9-protein-purification-protocol-dijv4cn6 | Shu Huang | TITLE: Cas9 Protein Purification Protocol
AUTHORS: Shu Huang
[DESCRIPTION]
This protocol outlines the steps for purifying Cas9 protein, essential for the preparation of RNP complexes used in CRISPR-Cas9 editing processes.
[STEPS]
SECTION: Expression of Cas9 protein in a suitable host system
1. Express the Cas9 protei... | ["[Expression of Cas9 protein in a suitable host system] Express the Cas9 protein in a host system that supports optimal protein yield and stability.", "[Lysis and purification of Cas9 protein] Lyse the host cells and purify the Cas9 protein using standard biochemical techniques to ensure high purity and activity.", "[... |
55,102 | Measuring Photophysiology of Attached Stages of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer | 3 | null | https://www.protocols.io/view/measuring-photophysiology-of-attached-stages-of-co-bz26p8he | Takehiro Kazama, Kazuhide Hayakawa, Koichi Shimotori, Akio Imai | TITLE: Measuring Photophysiology of Attached Stages of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer
AUTHORS: Takehiro Kazama, Kazuhide Hayakawa, Koichi Shimotori, Akio Imai
[DESCRIPTION]
Fast repetition rate fluorometer (FRRf) is a beneficial method for measuring photosystem II (PSII) photophysiolog... | [] |
103,693 | to export w comments | 0 | null | https://www.protocols.io/view/to-export-w-comments-dhhm3346 | Maria Gulyakina | TITLE: to export w comments
AUTHORS: Maria Gulyakina
[DESCRIPTION]
qa
[STEPS]
SECTION: s1
1. test
SECTION: s1
2. test 2
SECTION: s1
3. test
SECTION: s2
4. test
SECTION: s2
5. test 2
SECTION: s2
6. test 3
SECTION: s3
7. test
SECTION: s3
8. test 2 | ["[s1] test", "[s1] test 2", "[s1] test", "[s2] test", "[s2] test 2", "[s2] test 3", "[s3] test", "[s3] test 2"] |
null | null | null | dx.doi.org/10.17504/protocols.io.mn7c5hn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
92,911 | Cryo-electron tomography of thinned synapses | 1 | dx.doi.org/10.17504/protocols.io.n92ldm188l5b/v1 | https://www.protocols.io/view/cryo-electron-tomography-of-thinned-synapses-c6ypzfvn | Anna Siegert, Arsen Petrovic, Thanh Thao Do, Florelle Domart, Rubén Fernández-Busnadiego | TITLE: Cryo-electron tomography of thinned synapses
AUTHORS: Anna Siegert, Arsen Petrovic, Thanh Thao Do, Florelle Domart, Rubén Fernández-Busnadiego
[DESCRIPTION]
Here we provide a protocol for cryo-electron tomography (cryo-ET) of thinned synapses within intact rat primary neuron cultures. This workflow relies on cr... | ["[Culturing primary rat hippocampal neurons on EM grids - Preparation of the EM grids] Glow discharge the EM grids (SiO2 film on Au mesh, R 1/2 or R 2/2, Quantifoil) with a plasma cleaner (30 s, medium voltage) followed by 30 min under UV light.", "[Culturing primary rat hippocampal neurons on EM grids - Preparation o... |
82,108 | Loading a Radseq Library on Nextseq 500 | 4 | dx.doi.org/10.17504/protocols.io.kxygx9j1zg8j/v1 | https://www.protocols.io/view/loading-a-radseq-library-on-nextseq-500-cue4wtgw | Angie Chia | TITLE: Loading a Radseq Library on Nextseq 500
AUTHORS: Angie Chia
[DESCRIPTION]
Protocol to load a Radseq Library on EFGL Nextseq 500
[STEPS]
SECTION: NextSeq 500 Prep
2. Thaw Reagent Tray and Flow Cell - It is important that the output of both the reagent tray and flow cell matches i.e. MID/MID or HIGH/HIGH
SECTION... | ["[NextSeq 500 Prep] Thaw Reagent Tray and Flow Cell - It is important that the output of both the reagent tray and flow cell matches i.e. MID/MID or HIGH/HIGH", "[NextSeq 500 Prep] Retrieve a High-300 output or Mid-300 output Illumina Reagent Tray from the freezer and place in bucket/container of room temperature tap ... |
null | null | null | dx.doi.org/10.17504/protocols.io.gswbwfe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Cell viability can be assessed based on various cellular features and mechanisms. These include cell membrane integrity (detected by cell impermeable dyes or leakage of intracellular lactate dehydrogenase (LDH) activity), monitoring of ATP with bioluminescence assays, determinin... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.mmwc47e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A method allowing the simultaneous quantitation of 21 amino acids in dry leaf tissue using liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) in multiple reaction monitoring (MRM) mode.</p>
[BEFORE_START]
<p>Samples, standards and solvents need to be f... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.saceaaw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span style="display: inline !important; float: none; background-color: transparent; color: #000000; font-family: Lato,Verdana,Arial,sans-serif; font-size: 14px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; line-height: 20px; orphans: 2;... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.dpd5i5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol gives a method for isolating temperate phages from marine viral concentrations. This protocol uses the conventional plaque agar overlay and looking for turbid or haloed plaques, a hallmark of temperate phages.<br /><br />Paul, J. H., and M. Weinbauer. 2010. Detecti... | [] |
66,331 | Testo Edge X UK Male Booster Pills Review | 3 | dx.doi.org/10.17504/protocols.io.q26g74k8kgwz/v1 | https://www.protocols.io/view/testo-edge-x-uk-male-booster-pills-review-ccz3sx8n | perrinbureau | TITLE: Testo Edge X UK Male Booster Pills Review
AUTHORS: perrinbureau
[DESCRIPTION]
Testo Edge X
[STEPS] | [] |
49,101 | Intranuclear CITE-seq (inCITE-seq): joint single-cell measurements of multiplexed nuclear proteins and RNA | 4 | null | https://www.protocols.io/view/intranuclear-cite-seq-incite-seq-joint-single-cell-bt7mnrk6 | Hattie Chung, Emma Magee | TITLE: Intranuclear CITE-seq (inCITE-seq): joint single-cell measurements of multiplexed nuclear proteins and RNA
AUTHORS: Hattie Chung, Emma Magee
[DESCRIPTION]
This protocol allows for intranuclear antibody staining of fixed nuclei in suspension. Nuclei suspensions are suitable for CITE-seq and 10X Genomic applic... | ["[Fixation and Permeabilization] Transfer 1 mL of nuclei suspension from the filter tube into a pre-chilled 15mL Falcon tube.", "[Primary Antibody Stain] Resuspend pellet in 500 µL of Blocking Buffer. Pipette up and down multiple times using a P200 pipette to ensure the sample is mixed thoroughly.", "[Fixation and Per... |
81,979 | USDA LTAR Common Experiment measurement: Soil organic carbon stocks and change | 1 | dx.doi.org/10.17504/protocols.io.q26g7yo1kgwz/v1 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-soil-organ-cua3wsgn | SCarolina Córdova, Curtis Dell, Mark Liebig, Michel A Cavigelli, David R Huggins, G Philip Robertson | TITLE: USDA LTAR Common Experiment measurement: Soil organic carbon stocks and change
AUTHORS: SCarolina Córdova, Curtis Dell, Mark Liebig, Michel A Cavigelli, David R Huggins, G Philip Robertson
[DESCRIPTION]
The change in soil organic carbon (SOC) over time provides an integrated indicator of carbon (C) balance in a... | ["[Sample Collection] Define the objectives of your sampling. Are you conducting long-term monitoring or not? Decadal whole-profile soil sampling involves collecting samples from a predetermined set of sites regularly over the years. Surface sampling may have additional or different sampling sites. Select sites that ar... |
null | null | null | dx.doi.org/10.17504/protocols.io.guvbww6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 2">
<div class="section">
<div class="layoutArea">
<div class="column">
<p>The Odyssey Fc Imager, with 600 channel capabilities, can image agarose gels stained with popular DNA stains, such as ethidium bromide and SYBR Safe DNA stain, with sub-nanog... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.shseb6e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol refers to a study having the aim to identify and test at field artificial structures suitable as collectors for common cuttlefish <em>Sepia officinalis</em> eggs in wild condition.</p>
<p>During the same study, three different protocols were tested and compared.... | [] |
51,597 | Measuring Photophysiology of Attached Stages of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer | 3 | dx.doi.org/10.17504/protocols.io.bwmmpc46 | https://www.protocols.io/view/measuring-photophysiology-of-attached-stages-of-co-bwmmpc46 | Takehiro Kazama, Kazuhide Hayakawa, Koichi Shimotori, Akio Imai | TITLE: Measuring Photophysiology of Attached Stages of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer
AUTHORS: Takehiro Kazama, Kazuhide Hayakawa, Koichi Shimotori, Akio Imai
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Fast repetition rate fluorometer (FRRf) is a beneficia... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kyhcxt6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>HBV genotyping was performed by sequencing and phylogenetic analyses of the surface (S) and core (C) fragments. Briefly, HBV DNA was first extracted from 400 µL of plasma and eluted in 100 µL of pure water, using the QIAamp Viral DNA Mini Kit (QIAGEN, Courtaboeuf, France) fo... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kehctb6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
12,687 | Implanting PDX Tissue into SCID mice | null | dx.doi.org/10.17504/protocols.io.qmpdu5n | https://www.protocols.io/view/implanting-pdx-tissue-into-scid-mice-qmpdu5n | Lindsey Abel, Saakshi Kaushik, Kwangok P Nickel, Randall Kimple | TITLE: Implanting PDX Tissue into SCID mice
AUTHORS: Lindsey Abel, Saakshi Kaushik, Kwangok P Nickel, Randall Kimple
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for the establishment of patient derived xenograft from fresh patient tissue to SCID mouse or to passage from one mouse to ... | ["[Human to mouse implant- Preparation]\nPlace fresh tissue (should be passaged within 48 hours of removal from patient ; stored at 4 degrees C) in 60mm cell culture plate filled with 2-4 ml cold 1X PBS. Trim and clean tissue to remove areas of necrosis, fat, muscle.", "[Human to mouse implant- Preparation]\nWhile clea... |
97,512 | CODA (part 4): register the deep learning labelled images and Construct 3D tissue matrix | HuBMAP | JHU-TMC | 0 | dx.doi.org/10.17504/protocols.io.yxmvme7eog3p/v1 | https://www.protocols.io/view/coda-part-4-register-the-deep-learning-labelled-im-dbgg2jtw | Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz | TITLE: CODA (part 4): register the deep learning labelled images and Construct 3D tissue matrix | HuBMAP | JHU-TMC
AUTHORS: Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz
[DESCRIPTION]
CODA (part 4): register the deep learning labelled images and Construct 3D tissue matrix
[STEPS]... | ["[Register the deep learning labelled images] Using the registration transformations calculated in low-resolution (as described in CODA-part2), register the segmentation masks of the high-resolution tif images generated in CODA-part3.", "[Function requirements] High-resolution images from CODA-part3 (pthclassified)", ... |
null | null | null | dx.doi.org/10.17504/protocols.io.g3gbyjw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol can be used to analyze phosphate uptake by KaiC proteins over time.</p>
[BEFORE_START]
<p>prepare gels for <a href="https://www.protocols.io/view/lowc-sds-page-gysbxwe" target="_blank">LowC SDS-PAGE</a></p>
[GUIDELINES]
<p><strong>references:</strong></p>
<p> ... | [] |
95,775 | High content imaging of lysosomal phenotypes in iPSC-derived neurons using the Opera Phenix(TM) High-content Screening System | 0 | dx.doi.org/10.17504/protocols.io.14egn353yl5d/v1 | https://www.protocols.io/view/high-content-imaging-of-lysosomal-phenotypes-in-ip-c9r7z59n | Jessica Chedid, Nicolas Dzamko, Adahir Labrador-Garrido | TITLE: High content imaging of lysosomal phenotypes in iPSC-derived neurons using the Opera Phenix(TM) High-content Screening System
AUTHORS: Jessica Chedid, Nicolas Dzamko, Adahir Labrador-Garrido
[DESCRIPTION]
This protocol describes the use of the Opera Phenix high-content screening system and its advanced software... | ["[Methods section 1] Example analysis sequence: \n1. Lysosomal degradation capacity using DQ-red-BSA: \n \nStep 1: \nUsing the “find nuclei” analysis block, with the channel in which the nuclei are stained (we used Hoechst) the user can choose the most accurate method out of 4 available and adjust parameters like thr... |
26,540 | U Mass - Coronary Artery Ligation | null | dx.doi.org/10.17504/protocols.io.56kg9cw | null | Mark Kelly LAT, Timothy P. Fitzgibbons | TITLE: U Mass - Coronary Artery Ligation
AUTHORS: Mark Kelly LAT, Timothy P. Fitzgibbons
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">
This is a mouse model of acute myocardial infarction. This protocol is suitable f... | ["Expected procedure duration: 20-40 minutes", "Adequacy or depth of anesthesia is monitored by: Respiratory Rate and Toe Pinch", "Frequency of anesthesia depth assessment:At the start of surgical procedure, a toe or ear pinch can be used to assess the depth of anesthesia. Visual monitoring should be performed thought-... |
33,848 | Detection of JEV and WNV by quantitative real-time reverse-transcription (qRT)-PCR | 1 | dx.doi.org/10.17504/protocols.io.bdayi2fw | https://www.protocols.io/view/detection-of-jev-and-wnv-by-quantitative-real-time-bdayi2fw | Wenjing Liu, Shihong Fu | TITLE: Detection of JEV and WNV by quantitative real-time reverse-transcription (qRT)-PCR
AUTHORS: Wenjing Liu, Shihong Fu
[DESCRIPTION]
you can use this protocol to detect the RNA of your sample
[BEFORE_START]
RNA was extracted from all collected samples (serum, CSF, and mosquito grinding supernatant) using a T... | ["[Detection of JEV and WNV by quantitative real-time reverse-transcription (qRT)-PCR] The instrument used was a Stratagene ™, real time PCR machine (manufacturer: Thermo Scientific, model: MX300) for quantitative fluorescence qRT-PCR detection of JE virus and West Nile virus. AgPath-IDTMOne-step RT-PCR Kit (REF: AM100... |
63,912 | simpli acv keto gummies reviews for weight loss Pills: Everything Consumers Need to Know AboutPills Includes Apple Cider Vinegar goBHB Exogenous Ketones Advanced Ketogenic Supplement Ketosis Support for Men Women 60 Capsules | 4 | dx.doi.org/10.17504/protocols.io.yxmvmnqdog3p/v1 | https://www.protocols.io/view/simpli-acv-keto-gummies-reviews-for-weight-loss-pi-cangsdbw | simplyhealthacvketogummiesu , Simply Health ACV Keto Gummies | TITLE: simpli acv keto gummies reviews for weight loss Pills: Everything Consumers Need to Know AboutPills Includes Apple Cider Vinegar goBHB Exogenous Ketones Advanced Ketogenic Supplement Ketosis Support for Men Women 60 Capsules
AUTHORS: simplyhealthacvketogummiesu , Simply Health ACV Keto Gummies
[DESCRIPTION]
Si... | ["simpli acv keto gummies scam Reviews – Complete Ripoff or Keto Pills That Work?Real Scam Complaints or Legit Diet Pills?", "Simply Health ACV Keto Gummies\nProduct Review: — Simply Health ACV Keto GummiesUsed For: — 🔶Weight Loss\n🔶Health Benefits\n🔶Burn excess fat\n🔶Better gut health & promote digestion\n🔶Impro... |
90,733 | Stable cell line generation via retrovirus | 4 | dx.doi.org/10.17504/protocols.io.81wgbxq2qlpk/v1 | https://www.protocols.io/view/stable-cell-line-generation-via-retrovirus-c4umywu6 | Dan Tudorica | TITLE: Stable cell line generation via retrovirus
AUTHORS: Dan Tudorica
[DESCRIPTION]
Production of pseudotyped virus an subsequent transduction.
[STEPS]
1.
Day 0: Thaw fresh cryovial of cells to
be transduced
Thaw ~ 1 week prior to planned transduction day, use
low passage number if possible. Aim for cells to be... | ["Day 0: Thaw fresh cryovial of cells to\nbe transduced\n\n\nThaw ~ 1 week prior to planned transduction day, use\nlow passage number if possible. Aim for cells to be 80% confluent on day of transduction.\nRecommend seeding four wells of a 12-well plate (100,000 cells/well) the day\nbefore transduction (after collectin... |
33,174 | Marchantia genotyping (quick and dirty genomic DNA extraction) | null | dx.doi.org/10.17504/protocols.io.bcmwiu7e | null | Eftychis Frangedakis, marta tomaselli, Marius Rebmann, Susana Sauret-Gueto | TITLE: Marchantia genotyping (quick and dirty genomic DNA extraction)
AUTHORS: Eftychis Frangedakis, marta tomaselli, Marius Rebmann, Susana Sauret-Gueto
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol allows for quick and dirty genomic DNA extraction. It can easily be used for genotyp... | ["Take small pieces (3x3 mm) of thalli from individual plants and place in a 1.5 mL Eppendorf tube (A in Figure).", "Add 100 μl of genotyping buffer,: 100 mM Tris-HCl pH 9.5, 1M KCl, 10 mM EDTA (B in Figure).", "Crush with an autoclaved micro-pestle (C in Figure).", "Place the tube(s) at 80 °C for 10 min.", "Add 380 μ... |
31,082 | Light microscopic analysis of synaptic input to neurons in the rat major pelvic ganglion [keast-003] | null | dx.doi.org/10.17504/protocols.io.bakiicue | https://www.protocols.io/view/light-microscopic-analysis-of-synaptic-input-to-ne-bakiicue | Janet Keast, Peregrine Osborne | TITLE: Light microscopic analysis of synaptic input to neurons in the rat major pelvic ganglion [keast-003]
AUTHORS: Janet Keast, Peregrine Osborne
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This collection describes the procedures required to visualise and characterize synaptic boutons associa... | [] |
42,990 | Dv Control Media (SIlver Cap) | 4 | dx.doi.org/10.17504/protocols.io.bm8nk9ve | https://www.protocols.io/view/dv-control-media-silver-cap-bm8nk9ve | Catherine Gohar, Ada de la Cruz | TITLE: Dv Control Media (SIlver Cap)
AUTHORS: Catherine Gohar, Ada de la Cruz
[STEPS]
?. [Equipment for making media]
For making media you will need: 2 --> 500 mL graduated cylinders1 --> 100 mL graduated cylinder1 --> 10 mL graduated cylinder2 --> small funnels2 --> weigh boats1 --> lab spatula 1 --> pipette + tip1 -... | ["[Equipment for making media]\nFor making media you will need: 2 --> 500 mL graduated cylinders1 --> 100 mL graduated cylinder1 --> 10 mL graduated cylinder2 --> small funnels2 --> weigh boats1 --> lab spatula 1 --> pipette + tip1 --> 1000 mL or 2000 mL flask (depending on if you're making 1 L or 2 L of media)", "[Mak... |
86,876 | U54 SCENT Adult Colonoscopy Tissue Collection Procedure | 1 | dx.doi.org/10.17504/protocols.io.5jyl8ppz7g2w/v1 | https://www.protocols.io/view/u54-scent-adult-colonoscopy-tissue-collection-proc-cy34xyqw | Katherine Garman | TITLE: U54 SCENT Adult Colonoscopy Tissue Collection Procedure
AUTHORS: Katherine Garman
[DESCRIPTION]
This protocol outlines the inclusion and exclusion criteria as well as collection and processing of adult colon tissue at Duke University
[STEPS]
SECTION: General Guidelines
1. 1. Biobanking will proceed through the... | ["[General Guidelines] 1. Biobanking will proceed through the Duke GI Tissue Repository (PRO00001662), established to screen patients presenting for upper endoscopy and colonoscopy in Duke GI. The Duke GI Tissue Repository already partners with BRPC for sample processing and discernment.\n\n2. Weekly screening of the e... |
26,688 | SPARC - Gastrointestinal myoelectric recordings from the behaving ferret | null | dx.doi.org/10.17504/protocols.io.6a8hahw | null | Charles C. Horn, Derek M. Miller, Stephanie Fulton, Bill J. Yates, Lee E. Fisher, Ameya C. Nanivadekar | TITLE: SPARC - Gastrointestinal myoelectric recordings from the behaving ferret
AUTHORS: Charles C. Horn, Derek M. Miller, Stephanie Fulton, Bill J. Yates, Lee E. Fisher, Ameya C. Nanivadekar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol provides the steps for recording the gastroint... | ["[Room setup]\nCover floor of behavioral test chamber with layer of Alpha-dri bedding. The floor of the plexiglas chamber is 51 x 51 cm (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348962/)", "[Room setup]\nTurn off all lights except lamp over testing chamber.", "[Room setup]\nKeep the room quiet during recording ex... |
63,076 | In Vivo Electrophysiology (Mouse) | 4 | dx.doi.org/10.17504/protocols.io.b9ucr6sw | https://www.protocols.io/view/in-vivo-electrophysiology-mouse-b9ucr6sw | Alexandra Nelson | TITLE: In Vivo Electrophysiology (Mouse)
AUTHORS: Alexandra Nelson
[DESCRIPTION]
This protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.
[STEPS]
1. Making Optrodes.
2. Implant surgery.
(Refer to the surgery protocol for full surgical details: dx.doi.org/10.17504/proto... | ["Making Optrodes.", "Implant surgery. \n(Refer to the surgery protocol for full surgical details: dx.doi.org/10.17504/protocols.io.n2bvj6qynlk5/v1) \nFor implantation of electrode arrays with or without an attached optical fiber, you will cut three holes: one at the site of the array implant (left side), one in the ri... |
41,433 | HuBMAP TMC-UF Validation of Custom conjugated Antibodies for CODEX | 1 | dx.doi.org/10.17504/protocols.io.bkpzkvp6 | https://www.protocols.io/view/hubmap-tmc-uf-validation-of-custom-conjugated-anti-bkpzkvp6 | Marda Jorgensen, Jerelyn Nick | TITLE: HuBMAP TMC-UF Validation of Custom conjugated Antibodies for CODEX
AUTHORS: Marda Jorgensen, Jerelyn Nick
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the methods for validating antibodies after custom conjugation with Akoya CODEX barcodes in FFPE tissues on coversl... | ["[Tissue Pre-treatment ]\nTurn on the heating plate and set it at 55°C.\n55 °C", "Once the heating plate has reached 55°C, retrieve the FFPE samples on poly-l-Lysine treated coverslip(s) from 4°C storage.\n4 °C", "Using bent tip forceps, place the sample coverslip(s) on the hot plate with the tissue facing up. In... |
70,098 | Confocal imaging on Nikon AXR confocal microscope | 4 | dx.doi.org/10.17504/protocols.io.rm7vzbn9rvx1/v1 | https://www.protocols.io/view/confocal-imaging-on-nikon-axr-confocal-microscope-cgpstvne | Andrew Hunter | TITLE: Confocal imaging on Nikon AXR confocal microscope
AUTHORS: Andrew Hunter
[DESCRIPTION]
This protocol provides a step by step protocol to acquire images on Nikon AXR confocal set-up.
[STEPS]
SECTION: Confocal set-up
1. In this order: Turn on power to microscope stand (15seconds to power up), power on the contr... | ["[Confocal set-up] In this order: Turn on power to microscope stand (15seconds to power up), power on the controller by pushing the button and waiting for the standby lights to go green, then provide power to the lasers by turning the activation key", "[Confocal set-up] If using CAM, begin reservation to receive acces... |
58,764 | Maternal Lines Protocol | 3 | null | https://www.protocols.io/view/maternal-lines-protocol-b5mkq44w | Meghan Duffy, Rebecca Bilich | TITLE: Maternal Lines Protocol
AUTHORS: Meghan Duffy, Rebecca Bilich
[DESCRIPTION]
This a protocol to set up and maintain Daphnia maternal lines for clones that will be used in experiments.
[STEPS] | [] |
27,135 | Analysis of co-expression networks among Populus spp. involved in the response to phosphate deficiency and aluminium toxicity | null | dx.doi.org/10.17504/protocols.io.6q7hdzn | null | Thiago Bergamo Cardoso, Renan Terassi Pinto, Luciano Vilela Paiva | TITLE: Analysis of co-expression networks among Populus spp. involved in the response to phosphate deficiency and aluminium toxicity
AUTHORS: Thiago Bergamo Cardoso, Renan Terassi Pinto, Luciano Vilela Paiva
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:l... | ["[Analysis of data from microarray experiments]\nThe first step was to download the data and perform the necessary analyzes to determine which genes were differentially expressed.", "[Analysis of data from microarray experiments]\nThe microarray data used in this work were obtained from the NCBI database using accessi... |
63,937 | Slim Fast Keto Reviews: Is Keto Pills Really Works? | 1 | dx.doi.org/10.17504/protocols.io.dm6gpbqe8lzp/v1 | https://www.protocols.io/view/slim-fast-keto-reviews-is-keto-pills-really-works-can9sdh6 | Express Keto Pills Reviews | TITLE: Slim Fast Keto Reviews: Is Keto Pills Really Works?
AUTHORS: Express Keto Pills Reviews
[DESCRIPTION]
Slim Fast Keto Reviews: Is Keto Pills Really Works?
[STEPS]
SECTION: Slim Fast Keto Reviews: Is Keto Pills Really Works? Everyone wants to lose weight presto, numerous people are trying to get their dream bod... | ["[Slim Fast Keto Reviews: Is Keto Pills Really Works? Everyone wants to lose weight presto, numerous people are trying to get their dream body, and to some, it seems like a far- brought dream. Occasionally, losing too important weight too presto may be dangerous to your health. For this reason, the world has embraced... |
66,334 | Wonder CBD Oil :Quit Smoking and 100% Pain Relief! | 3 | dx.doi.org/10.17504/protocols.io.6qpvr6ndbvmk/v1 | https://www.protocols.io/view/wonder-cbd-oil-quit-smoking-and-100-pain-relief-ccz6sx9e | marlenedrobbins | TITLE: Wonder CBD Oil :Quit Smoking and 100% Pain Relief!
AUTHORS: marlenedrobbins
[DESCRIPTION]
WONDER Leaf cbd oil is a unique product that mixes natural remedies to aid individuals in residing a more fit lifestyle.
[STEPS] | [] |
73,969 | Analysis of the effect of wind speed in increasing the COVID-19 cases in Jakarta | 1 | dx.doi.org/10.17504/protocols.io.ewov1odzylr2/v1 | https://www.protocols.io/view/analysis-of-the-effect-of-wind-speed-in-increasing-ckgrutv6 | Dewi Susanna, Yoerdy Agusmal Saputra, Sandeep Poddar | TITLE: Analysis of the effect of wind speed in increasing the COVID-19 cases in Jakarta
AUTHORS: Dewi Susanna, Yoerdy Agusmal Saputra, Sandeep Poddar
[DESCRIPTION]
Background:
COVID-19 remains public health problem around the world. It is possible the climate could affect the transmission of COVID-19.Wind is one of t... | ["[Methods] Study design\nA quantitative method was applied with an ecological design that provides real-time and location analysis, using geographic information systems, and the data were tested using statistical techniques.", "[Methods] Data collection\nThe secondary data comprised daily reports of COVID-19 infection... |
33,953 | Porcine Circovirus 3 (PCV3) Complete Genome Sequence PCR | 1 | dx.doi.org/10.17504/protocols.io.bdd9i296 | https://www.protocols.io/view/porcine-circovirus-3-pcv3-complete-genome-sequence-bdd9i296 | Chew Yee Tan | TITLE: Porcine Circovirus 3 (PCV3) Complete Genome Sequence PCR
AUTHORS: Chew Yee Tan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes four separate PCR runs to amplify the complete genome sequence of Porcine circovirus 3 (PCV3) as four overlapping segments. </div></div>
[STE... | ["DNA extraction was performed using DNeasy Blood & Tissue Kit extraction kit (Qiagen, Germany) in accordance to manufacturer’s instructions. Samples involved include lung, inguinal lymph node, spleen, tonsil, kidney, mesenteric lymph node, heart, liver and brain tissues.", "Four separate conventional PCR runs were per... |
40,223 | Conjugation of Keyhole limpet haemocynin to Peptide 254-274 of HIV gp-120 as immunogen. | 6 | dx.doi.org/10.17504/protocols.io.bjh7kj9n | https://www.protocols.io/view/conjugation-of-keyhole-limpet-haemocynin-to-peptid-bjh7kj9n | Angel Justiz-Vaillant | TITLE: Conjugation of Keyhole limpet haemocynin to Peptide 254-274 of HIV gp-120 as immunogen.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Chemical synthesis facilitates the generation of peptides which are difficult to express in bacteria. The fragment 254-274 of... | ["The fragment 254-274 of gp 120 was conjugated by the glutaraldehyde method.", "One mg of keyhole limpet hemocyanin (KLH) is dissolved in 2 ml 0.1 M borate buffer (1.24 g boric acid, 1.90 g sodium tetraborate, pH 10, in 500 mL deionized water).", "In a 20 ml glass tube by gentle stirring 1 µmol of the HIV synthetic pe... |
99,431 | Nanopore Library Preparation for R10 Ligation Sequencing Kit | 4 | dx.doi.org/10.17504/protocols.io.j8nlkozy5v5r/v2 | https://www.protocols.io/view/nanopore-library-preparation-for-r10-ligation-sequ-ddcf22tn | Guan Jie Phang | TITLE: Nanopore Library Preparation for R10 Ligation Sequencing Kit
AUTHORS: Guan Jie Phang
[DESCRIPTION]
Protocols for Nanopore R10 flow cell Ligation Sequencing library construction.
[STEPS]
SECTION: Step1. End-prep
1. In a 200 µL PCR tube, mix the following chemicals:
SECTION: Step1. End-prep
1.1. Incub... | ["[Step1. End-prep] In a 200 µL PCR tube, mix the following chemicals:", "[Step1. End-prep] Incubate in a thermocycler at 20 °C for 30 min and 65 °C for 30 min. Hold at 4 °C", "[Step2. Adapter ligation] Thaw the Ligation Adapter (LA) and Ligation Buffer (LNB). Vortex and spin down the tubes, and place on ice immediatel... |
null | null | null | dx.doi.org/10.17504/protocols.io.s9qeh5w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The <em>in vitro </em>α-glucosidase inhibitory assay was performed according to Dineshkumar et al. [8]. The α-glucosidase enzyme (2 U/mL) was premixed with 20 µL of plant aqueous extract at a concentration of 1,000 µg/mL and incubated for 5 min at 37 °C. Then 1 mM p-nitrophen... | [] |
107,628 | Microwave synthesis of low molecular weight deacylated chitosan | 6 | dx.doi.org/10.17504/protocols.io.x54v9d6w4g3e/v4 | https://www.protocols.io/view/microwave-synthesis-of-low-molecular-weight-deacyl-dmck42uw | Maria J Torres, Eric S McLamore, Geisianny AM Moreira | TITLE: Microwave synthesis of low molecular weight deacylated chitosan
AUTHORS: Maria J Torres, Eric S McLamore, Geisianny AM Moreira
[DESCRIPTION]
This protocol describes the synthesis of low molecular weight deacylated chitosan using microwave irradiation. The process requires approximately 2.5 hours (including base... | ["[SECTION 1) Materials and solution preparation] Prepare reaction vials\n\nCheck the vial cap and Teflon liner to make sure neither is damageddamage to the cap and both are clean (Fig 1A-B)", "[SECTION 1) Materials and solution preparation] Add solution to the reaction vial.\n\nPipette 8000 µL of 1% deacylated chitosa... |
35,142 | Operating an OT-2 for COVID-19 testing | null | dx.doi.org/10.17504/protocols.io.bejejcje | null | Max Marrone | TITLE: Operating an OT-2 for COVID-19 testing
AUTHORS: Max Marrone
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. Open the Opentrons App.
?. Click Start run. The OT-2 will home its motors and then begin the protocol.
Do not click Start run more than once. If you do, a known bug will make the OT-2 run the ... | ["Open the Opentrons App.", "Click Start run. The OT-2 will home its motors and then begin the protocol.\nDo not click Start run more than once. If you do, a known bug will make the OT-2 run the protocol back-to-back.\nIf something goes wrong and you need to abort the protocol:Shut down the OT-2 with the power switch o... |
null | null | null | dx.doi.org/10.17504/protocols.io.hhbb32n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<div>
<div>
<p>This protocol is from 'Flower Ca<sup>2+</sup> channel in CME and ADBE' of Yao CK et al.</p>
<div>
<div>
<p> </p>
<p>Please see the manuscript for the full method details.</p>
</div>
</div>
</div>
</div>
</div>
[BEFORE_START]
<p>You'll need: </p>
<p> </p>
<p... | [] |
82,997 | Incidence of postpartum hemorrhage based on the improved combined method in evaluating blood loss | 1 | dx.doi.org/10.17504/protocols.io.j8nlkobewv5r/v1 | https://www.protocols.click/view/incidence-of-postpartum-hemorrhage-based-on-the-im-cvavw2e6 | Fangyuan Zheng, Haiyan Wen | TITLE: Incidence of postpartum hemorrhage based on the improved combined method in evaluating blood loss
AUTHORS: Fangyuan Zheng, Haiyan Wen
[DESCRIPTION]
In view of the current clinical inaccuracies and underestimations of postpartum hemorrhage amount, this study aims to investigate the incidence, etiology, clinical ... | ["[Design] This retrospective cohort study was conducted in Hangzhou Women's Hospital from January 2020 to June 2021. Based on different modes of delivery, the participants were divided into three groups: vaginal delivery, forceps delivery, and cesarean section, for comparison. Blood loss, incidence, and causes of post... |
62,478 | Aggregated aSyn Dot Blot assay | 1 | dx.doi.org/10.17504/protocols.io.261gen2xdg47/v1 | https://www.protocols.io/view/aggregated-asyn-dot-blot-assay-b89nrz5e | rabdelha | TITLE: Aggregated aSyn Dot Blot assay
AUTHORS: rabdelha
[DESCRIPTION]
This protocol details aggregated aSyn Dot Blot assay.
[STEPS]
SECTION: Aggregated aSyn Dot Blot assay
1. Normalize protein samples to equal concentrations between 100 ng-1000 ng/μl in PBS.
SECTION: Aggregated aSyn Dot Blot assay
2. Carefully spo... | ["[Aggregated aSyn Dot Blot assay] Normalize protein samples to equal concentrations between 100 ng-1000 ng/μl in PBS.", "[Aggregated aSyn Dot Blot assay] Carefully spot 1.5 µL each sample onto dry nitrocellulose ( pores) membrane.", "[Aggregated aSyn Dot Blot assay] Allow moisture to dry for a minute or two.", "[Aggr... |
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