id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
|---|---|---|---|---|---|---|---|
null | null | null | dx.doi.org/10.17504/protocols.io.c7yzpv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
In tangential flow filtration (TFF), the sample is run parallel to the filter. Particles that are smaller than the pore size are pushed out through the holes (the filtrate). Particles that are larger than the pore size are retained inside the filter (the retentate). TFF is most ... | [] |
100,807 | TruAI Neuromelanin Quantification | 4 | dx.doi.org/10.17504/protocols.io.5qpvo345bv4o/v2 | https://www.protocols.io/view/truai-neuromelanin-quantification-depf3djn | Anastasia Filimontseva, Zac Chatterton, Glenda Halliday, YuHong Fu | TITLE: TruAI Neuromelanin Quantification
AUTHORS: Anastasia Filimontseva, Zac Chatterton, Glenda Halliday, YuHong Fu
[DESCRIPTION]
Quantification of area and optical density of intracellular neuromelanin with TruAI.
[STEPS]
SECTION: Loading training label function in TruAI software
1. Open scanned images with Olympu... | ["[Loading training label function in TruAI software] Open scanned images with Olympus VS200 Desktop (EVIDENT Technology GmbH, ver. 4.1.1 build 29408).", "[Loading training label function in TruAI software] Under the ‘Detect’ window, select ‘Training Labels’.", "[Creating NM foreground training label class] Create a ne... |
64,644 | Production of 6x DNA Loading Dye | 4 | null | https://www.protocols.io/view/production-of-6x-dna-loading-dye-cbdcsi2w | Stephane Fadanka, Nadine Mowoh | TITLE: Production of 6x DNA Loading Dye
AUTHORS: Stephane Fadanka, Nadine Mowoh
[DESCRIPTION]
The process of gel electrophoresis involves separating DNA fragments using an electrical current while tracking the rate of molecular movement through a filtering gel.
Adding blue or orange tracking dye to colourless DNA ... | ["[Preparation of Reagent stocks] Preparing 20 % (v/v)Trehalose (100ml)\n\nAccurately weigh 20 g of powder into a 500 mLbeaker\nUse a measuring cylinder to measure100 mLof sterile distilled water into the beaker and stir gently until the powder is completely dissolved. \nTransfer the content into a clean Duran bottle... |
null | null | null | dx.doi.org/10.17504/protocols.io.mgcc3sw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span style="font-weight: 400;">Immunohistochemistry (IHC) is a method that combines biochemical, histological and immunological techniques into a simple but powerful assay for protein detection. IHC provides valuable information as it visualizes the distribution and localiza... | [] |
88,545 | DNA Cloning (Gibson Assembly, Transformation, Plating and Incubation) | 1 | dx.doi.org/10.17504/protocols.io.8epv5xnddg1b/v2 | https://www.protocols.io/view/dna-cloning-gibson-assembly-transformation-plating-c2p9ydr6 | NUS iGEM | TITLE: DNA Cloning (Gibson Assembly, Transformation, Plating and Incubation)
AUTHORS: NUS iGEM
[DESCRIPTION]
2023 NUS-Singapore iGEM Team followed this protocol to construct the plasmid of interest or to clone the plasmid of interest.
[STEPS]
SECTION: Gibson Assembly
1. Calculate the volumes of respective DNA fragmen... | ["[Gibson Assembly] Calculate the volumes of respective DNA fragments to assemble based on their length and concentration. (The maximum final volume of the mixed fragments is 5 µL for each reaction.)", "[Gibson Assembly] Add the DNA fragments into a PCR tube according to the volumes obtained from the calculation.", "[G... |
77,582 | Set Up Biodata Resource Inventory in Google Colab | 5 | dx.doi.org/10.17504/protocols.io.5jyl89o36v2w/v3 | https://www.protocols.io/view/set-up-biodata-resource-inventory-in-google-colab-cpznvp5e | Kenneth Schackart | TITLE: Set Up Biodata Resource Inventory in Google Colab
AUTHORS: Kenneth Schackart
[DESCRIPTION]
This protocol will guide you on how to get everything in place to update the Biodata Resource Inventory.
This protocol describes how to setup Google Colab, connect your Google Drive, and clone the repository.
Some of th... | ["[Prepare Google Drive] In your Google Drive home directory, create a new folder called GitHub.", "[Connect Colab to Drive] Go to Google Colab.\n\nIf you need to change the account you are using, close the pop up by clicking Cancel at the bottom right. Change your Google account by clicking the icon at the top right."... |
26,177 | Effects of Caloric Restriction Associated with Low-Impact Aerobics and Strength Training Exercises on Body Compositions and Metabolic Syndrome Components among Obese students | 1 | dx.doi.org/10.17504/protocols.io.5s9g6h6 | https://www.protocols.io/view/effects-of-caloric-restriction-associated-with-low-5s9g6h6 | Mohamed Ahmed Said, Mohamed Abdelmoneem, Mohamed Chaab Alibrahim, Moustafa Ahmed Elsebee, Ahmed Abdel Hamed Kotb. | TITLE: Effects of Caloric Restriction Associated with Low-Impact Aerobics and Strength Training Exercises on Body Compositions and Metabolic Syndrome Components among Obese students
AUTHORS: Mohamed Ahmed Said, Mohamed Abdelmoneem, Mohamed Chaab Alibrahim, Moustafa Ahmed Elsebee, Ahmed Abdel Hamed Kotb.
[DESCRIPTION]
... | [] |
58,052 | E. coli Culture | 1 | dx.doi.org/10.17504/protocols.io.5jyl899o7v2w/v1 | https://www.protocols.io/view/e-coli-culture-b4xcqxiw | Bailey Clark | TITLE: E. coli Culture
AUTHORS: Bailey Clark
[DESCRIPTION]
The abstract will be added later.
[BEFORE_START]
A video if unfamiliar: https://youtu.be/TWt62pgAEh0
[STEPS]
SECTION: Medium and Antibiotic Preparation
1. Add 2 mL (3 mL also acceptable) of LB medium and the appropriate antibiotic that selects for the targ... | ["[Medium and Antibiotic Preparation] Add 2 mL (3 mL also acceptable) of LB medium and the appropriate antibiotic that selects for the target strain into a 15 mL tube.", "[Medium and Antibiotic Preparation] Place the 15mL tube in the ThermoMixer at 37°C, 300 rpm, for 5 min .", "[Retrieving Cells] Gather cells.", "[Ret... |
23,883 | Pancreatic Insulin Content by Acid-Ethanol Extraction | null | dx.doi.org/10.17504/protocols.io.3jjgkkn | null | Ed Leiter | TITLE: Pancreatic Insulin Content by Acid-Ethanol Extraction
AUTHORS: Ed Leiter
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Used to calculate the insulin content in the pancreas.</div><div class = "text-block"><spa... | ["¼ - ½ of the pancreas is placed into 5 ml Acid-Ethanol (1.5% HCl in 70% EtOH) in a 15 ml conical vial.", "Incubate O/N at -20ºC.", "Homogenize tissue (I use a Polytron homogenizer).", "Incubate O/N at -20ºC.", "Centrifuge at 2000 rpm 15 min at 4ºC (Sorvall RT6000).", "Transfer aqueous solution to a new 15 ml conical ... |
28,708 | Transformation of Chemically Competent Cells | null | dx.doi.org/10.17504/protocols.io.8achsaw | null | NUS iGEM | TITLE: Transformation of Chemically Competent Cells
AUTHORS: NUS iGEM
[STEPS]
?. Add of miniprep plasmid or of Gibson product into competent cell
1 µl
5 µl
?. Incubate cells in water bath for
42 °C
?. Incubate cells on ice for
?. Add of LB media into cell sample
1 ml
?. Incubate cells at for
37 °C
?. Spin down th... | ["Add of miniprep plasmid or of Gibson product into competent cell\n1 µl\n5 µl", "Incubate cells in water bath for\n42 °C", "Incubate cells on ice for", "Add of LB media into cell sample\n1 ml", "Incubate cells at for\n37 °C", "Spin down the cells at for\nCentrifuge: 6000 34", "Remove of the supernatant\n700 µl"... |
105,146 | Assessing Geotactic and Phototactic Responses in Parhyale hawaiensis as Indicators of Environmental Pollutant Exposure | 0 | dx.doi.org/10.17504/protocols.io.x54v92zwml3e/v1 | https://www.protocols.io/view/assessing-geotactic-and-phototactic-responses-in-p-diw24fge | Ibrahim Lawan | TITLE: Assessing Geotactic and Phototactic Responses in Parhyale hawaiensis as Indicators of Environmental Pollutant Exposure
AUTHORS: Ibrahim Lawan
[DESCRIPTION]
This protocol presents a series of cost-effective behavioural assays tailored to evaluate the sub-lethal impacts of environmental pollutants on the marine a... | ["[Introduction] Environmental pollutants, including organic contaminants, heavy metals, and pesticides, are pervasive in aquatic ecosystems and pose significant risks to marine organisms. Sub-lethal exposure to these pollutants can lead to alterations in behavioural patterns, serving as early indicators of neurotoxic ... |
45,469 | HuBMAP Tissue Preservation Protocol v2 | 1 | dx.doi.org/10.17504/protocols.io.bqm5mu86 | https://www.protocols.io/view/hubmap-tissue-preservation-protocol-v2-bqm5mu86 | Yiing Lin, Shin Lin | TITLE: HuBMAP Tissue Preservation Protocol v2
AUTHORS: Yiing Lin, Shin Lin
[STEPS]
?. HuBMAP Tissue Preservation ProtocolVersion 2General Note: Keep collected tissues cooled on ice as much as possible to minimize warm ischemic damage prior to freezing and storage.Flash freezing tissues1. Place metal block into bucket ... | ["HuBMAP Tissue Preservation ProtocolVersion 2General Note: Keep collected tissues cooled on ice as much as possible to minimize warm ischemic damage prior to freezing and storage.Flash freezing tissues1. Place metal block into bucket of ice to cool.2. Place disposable petri dish onto the metal block to cool; use this... |
null | null | null | dx.doi.org/10.17504/protocols.io.dqe5td | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Transformation of heat-shock competent E. coli cells. Adapted to fit he protocol followed by Northeastern_Boston
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gukbwuw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Purpose:</strong> To evaluate the influence of nutrient availability on cellular elemental composition (quotas and stoichiometry) in marine picoeukaryotes.</p>
<p><strong>Summary:</strong> Elemental quotas and ratios are assessed under nutrient replete and deplete con... | [] |
65,253 | Myco Nootropic Brain Gummies Reviews – Does This Product Really Work? | 3 | dx.doi.org/10.17504/protocols.io.8epv595x4g1b/v1 | https://www.protocols.io/view/myco-nootropic-brain-gummies-reviews-does-this-pro-cbydsps6 | michaelcollinsa | TITLE: Myco Nootropic Brain Gummies Reviews – Does This Product Really Work?
AUTHORS: michaelcollinsa
[DESCRIPTION]
MycoMode Nootropic Brain Gummies
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.dsc6av | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
If one has a purified stock of viruses obtained, for example, by banding in a buoyant density gradient, or even a relatively pure viral concentrate obtained by size fractionation, it is possible to release the DNA in a high molecular weight form suitable for some applications (e... | [] |
42,727 | FR-Match: cell type matching for scRNAseq data | 5 | dx.doi.org/10.17504/protocols.io.bmyfk7tn | https://www.protocols.io/view/fr-match-cell-type-matching-for-scrnaseq-data-bmyfk7tn | Yun Renee Zhang, Brian Aevermann, Richard Scheuermann | TITLE: FR-Match: cell type matching for scRNAseq data
AUTHORS: Yun Renee Zhang, Brian Aevermann, Richard Scheuermann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">FR-Match is a supervised cell phenotype matching strategy for cluster-to-cluster cell transcriptome integration across scRNAseq experim... | ["[Data preparation and exploration]\nUse the built-in data preparation function to create data objects with required and optional input data elements. Create data objects for experiment 1 (E1) and experiment 2 (E2)", "[Data preparation and exploration]\nView comparative cell cluster sizes.", "[Data preparation and exp... |
101,267 | Pole Test | 0 | dx.doi.org/10.17504/protocols.io.4r3l2qqj3l1y/v1 | https://www.protocols.io/view/pole-test-de5t3g6n | daniel.dautan daniel, Per Svenningsson | TITLE: Pole Test
AUTHORS: daniel.dautan daniel, Per Svenningsson
[DESCRIPTION]
Test for motor ability in mice
[STEPS]
1. Place the wooden, 50 cm long, 1cm in diameter the home cage to be tested, and make sure it stands upright on stand.
2. Set up recording camera to record the trials.
3. Habituate the mice to the pol... | ["Place the wooden, 50 cm long, 1cm in diameter the home cage to be tested, and make sure it stands upright on stand.", "Set up recording camera to record the trials.", "Habituate the mice to the pole one time before testing by carefully placing the mouse on the top of the pole facing downwards. Allow the mouse to trav... |
98,106 | Enzymatic liver dissociation (with liver perfusion kit) | 0 | dx.doi.org/10.17504/protocols.io.261ge5o57g47/v1 | https://www.protocols.io/view/enzymatic-liver-dissociation-with-liver-perfusion-db222qge | Dorien De Pooter, Ben De Clerck, Koen Dockx, Domenica De Santis, Sarah Sauviller, Pascale Dehertogh, Matthias Beyens, Isabelle Bergiers, Isabel Nájera, Ellen Van Gulck, Nádia Conceição-Neto, Wim Pierson | TITLE: Enzymatic liver dissociation (with liver perfusion kit)
AUTHORS: Dorien De Pooter, Ben De Clerck, Koen Dockx, Domenica De Santis, Sarah Sauviller, Pascale Dehertogh, Matthias Beyens, Isabelle Bergiers, Isabel Nájera, Ellen Van Gulck, Nádia Conceição-Neto, Wim Pierson
[DESCRIPTION]
This protocol details enzymati... | ["[Reagent preparation] Storage\n\nLyophilized enzymes should be stored at 2 °C–8 °C. Buffer P (20x), Reagent C and Reagent E should be stored at Room temperature. Lyophilized enzymes should be reconstituted before the date indicated on their respective vials.", "[Reagent preparation] Reconstitution buffer", "[Reagent ... |
null | null | null | dx.doi.org/10.17504/protocols.io.kaucsew | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>ADAMTS5, the main aggrecanase, is a protein that seems to play a functional role in the development of brown and white adipose tissue (WAT) and browning of WAT. These published observations were made using the ADAMTS5-P mice originally generated by Pfizer, in collaboration wit... | [] |
18,087 | DNA extraction (Salting out) | null | dx.doi.org/10.17504/protocols.io.vwfe7bn | null | Karen Guimaraes | TITLE: DNA extraction (Salting out)
AUTHORS: Karen Guimaraes
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS] | [] |
107,141 | Micro Volume Purification of His-Tagged Proteins with IMAC Ni-Charged Resin | 0 | dx.doi.org/10.17504/protocols.io.3byl49yejgo5/v1 | https://www.protocols.io/view/micro-volume-purification-of-his-tagged-proteins-w-dkvd4w26 | Syon Schlecht, Emily Gunderson, Ruthie Fowler, Takara Aguilar | TITLE: Micro Volume Purification of His-Tagged Proteins with IMAC Ni-Charged Resin
AUTHORS: Syon Schlecht, Emily Gunderson, Ruthie Fowler, Takara Aguilar
[DESCRIPTION]
This protocol describes a micro volume purification method for His-tagged proteins using IMAC with Ni-charged resin in a mini spin chromatography colum... | ["[Preparing and Equilibrating Column] Resuspend and pipette 200 µL into a", "[Preparing and Equilibrating Column] Wash the by adding 200 µL of distilled water", "[Preparing and Equilibrating Column] Centrifuge the at 1000 x g, 2 min and discard any collected buffer", "[Preparing and Equilibrating Column] Centrif... |
64,645 | Lifestyle Keto: Best Healthy Weight Loss Diet Plan, Read Now! | 1 | dx.doi.org/10.17504/protocols.io.ewov1n1o7gr2/v1 | https://www.protocols.io/view/lifestyle-keto-best-healthy-weight-loss-diet-plan-cbddsi26 | kalodzasp | TITLE: Lifestyle Keto: Best Healthy Weight Loss Diet Plan, Read Now!
AUTHORS: kalodzasp
[DESCRIPTION]
The ketogenic diet is a high-fat, low-carb diet that is successful in assisting individuals with getting thinner and keep it off. The eating routine works by Lifestyle Keto constraining the body to consume fat f... | [] |
92,256 | NanoTag | 4 | dx.doi.org/10.17504/protocols.io.kxygx34zkg8j/v1 | https://www.protocols.io/view/nanotag-c6b8zarw | Maria Dimitriu, Leonard Steg, Rodrigo Arzate-Mejia, Isabelle Mansuy | TITLE: NanoTag
AUTHORS: Maria Dimitriu, Leonard Steg, Rodrigo Arzate-Mejia, Isabelle Mansuy
[DESCRIPTION]
Background: Genome-wide profiling of DNA-protein interactions in cells can provide important information about mechanisms of gene regulation. Most current methods for genome-wide profiling of DNA-bound proteins su... | ["[Cell collection] Remove media from culture dish and wash with D-PBS.", "[Cell collection] Dissociate cells using Accutase and count cells.", "[Cell collection] Wash cells with 1 mL Wash buffer and resuspend cells at 1 million cells/mL of Wash buffer. 400 µL per sample. Transfer in a 2 mL tube. Use 400,000 cells per ... |
71,030 | Behavioral tests in rodents coupled with dopamine signaling manipulations | 4 | dx.doi.org/10.17504/protocols.io.261ge345jl47/v1 | https://www.protocols.io/view/behavioral-tests-in-rodents-coupled-with-dopamine-chkwt4xe | Chuyu Chen, Loukia Parisiadou | TITLE: Behavioral tests in rodents coupled with dopamine signaling manipulations
AUTHORS: Chuyu Chen, Loukia Parisiadou
[DESCRIPTION]
These two protocols describe behavioral tests associated with Parkinson’s disease phenotypes in mice. The striatal motor learning protocol (rotarod test) is a two-phase paradigm assessi... | ["[Rotarod test] General Rota-rod test is performed as follows:", "[Rotarod test] The rotarod apparatus (Panlab LE8205, Harvard Apparatus) equipped with a mouse rod (3 cm diameter)", "[Rotarod test] Set the rotarod with a start speed of 4 rpm, and set to 4–40 rpm acceleration over 300s", "[Rotarod test] Hold the mouse ... |
86,219 | qRT-PCR sample preparation | 4 | dx.doi.org/10.17504/protocols.io.4r3l22713l1y/v1 | https://www.protocols.io/view/qrt-pcr-sample-preparation-cyfjxtkn | Louise Uoselis | TITLE: qRT-PCR sample preparation
AUTHORS: Louise Uoselis
[DESCRIPTION]
Protocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
[STEPS]
1. Synthesise cDNA libraries from total RNA from each sample using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems), using Oligo... | ["Synthesise cDNA libraries from total RNA from each sample using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems), using Oligo(dT)20 primers.", "Place the synthesised libraries on ice, and dilute the libraries 1:3 by adding 60 µL of DEPC-treated H2O to each sample, pipetting up and down gently to mi... |
null | null | null | dx.doi.org/10.17504/protocols.io.dyt7wm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This solution is used in the coral genomic DNA extraction protocol.<br /><br />The following recipe is for 50 mL of CTAB mix. Store at -20°C.
[STEPS]
?.
?.
?.
?. | [] |
67,185 | 96-well plate R3 desalt and clean up protocol for mass spec analysis | 1 | dx.doi.org/10.17504/protocols.io.dm6gpbnqdlzp/v1 | https://www.protocols.io/view/96-well-plate-r3-desalt-and-clean-up-protocol-for-cdurs6v6 | ronan.ocualain, Staceywarwood, Davidknight, Emmakeevill | TITLE: 96-well plate R3 desalt and clean up protocol for mass spec analysis
AUTHORS: ronan.ocualain, Staceywarwood, Davidknight, Emmakeevill
[DESCRIPTION]
This protocol details the procedure of 96-well plate R3 desalt and clean up protocol for mass spec analysis.
[BEFORE_START]
Locate the following buffers and reagen... | ["[Preparing plates] Locate the white Corning FiltrEX 96-well plate you will be using for your peptide desalting.\nLocate a new clean ABgene 96 well plate for peptide collection. Label \"collection\" on it.\nThere should be a \"wash/waste\" plate provided in the R3 box in the orange trays.", "[Preparing plates] Place t... |
null | null | null | dx.doi.org/10.17504/protocols.io.t68erhw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol describes a methond of single nucleus isolaton from human skeletal muscle for snRNA sequencing.
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | ["Prepare 5ml Buffer A and 10ml Buffer B for each human sample", "Dissect all the hind limb muscles and place in a 35/60mm tissue culture dish with 0.5ml PBS.", "Remove the fat from muscle using a scissors, and wash the muscle with 1X PBS twice.", "Mechanically chop the muscle into 1mm pieces with a razor, mix the red ... |
52,184 | Coffee Protocol | 1 | dx.doi.org/10.17504/protocols.io.bw7yphpw | https://www.protocols.io/view/coffee-protocol-bw7yphpw | John Borghi | TITLE: Coffee Protocol
AUTHORS: John Borghi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is intended as a demonstration of what I like to call the </span><span style = "font-weight:bold;">Coffee Cup Rule</span><span> - Document more than you think is necessary and with greate... | ["[Make Coffee]\nMeasure out and . If the scale is out of batteries, 4 1/4 cups (US) of water and 8 tablespoons of coffee will work in a pinch. Use a measuring cup for the water not the cup measures on the side of the coffee pot. Those refer to \"cups of coffee\".\n[water]\n[ground coffee]", "[Make Coffee]\nPour the ... |
94,264 | Extraction and analysis of primary metabolites during Xanthomonas-Barley interaction | 4 | dx.doi.org/10.17504/protocols.io.5qpvo36wdv4o/v1 | https://www.protocols.io/view/extraction-and-analysis-of-primary-metabolites-dur-c8ayzsfw | Veronica Roman-Reyna, Nathaniel Heiden, Jules Butchacas, Hannah Toth, Jessica L. Cooperstone, Jonathan M. Jacobs | TITLE: Extraction and analysis of primary metabolites during Xanthomonas-Barley interaction
AUTHORS: Veronica Roman-Reyna, Nathaniel Heiden, Jules Butchacas, Hannah Toth, Jessica L. Cooperstone, Jonathan M. Jacobs
[DESCRIPTION]
Intercellular host-associated bacteria shape the chemistry of the living eukaryotic environ... | ["[Sample preparation] For sample preparation you need:\n- Barley cv. Morex seeds\n- Xanthomonas translucens pv. undulosa strain UPB513, \n- Xanthomonas translucens pv. translucent strain UPB886", "[Bacterial and mock inoculation] The youngest fully expanded leaf of the three-week-old plants was used for inoculations. ... |
70,814 | Spatial N-glycomics with MALDI-MSI for human kidney tissue | 1 | dx.doi.org/10.17504/protocols.io.8epv5j1m4l1b/v2 | https://www.protocols.io/view/spatial-n-glycomics-with-maldi-msi-for-human-kidn-chd6t29e | Dusan Velickovic, Kumar Sharma, Theodore Alexandrov, Chris Anderton | TITLE: Spatial N-glycomics with MALDI-MSI for human kidney tissue
AUTHORS: Dusan Velickovic, Kumar Sharma, Theodore Alexandrov, Chris Anderton
[DESCRIPTION]
This protocol describes the procedure to obtain high quality MALDI mass spectrometry images of N-linked glycans from formalin-fixed paraffin embedded tissue. Thi... | ["[Scope] This protocol describes the procedure to obtain high quality MALDI mass spectrometry images of N-linked glycans from formalin-fixed paraffin embedded tissue.", "[Health and Safety] Wear nitrile gloves and safety glasses. Follow standard laboratory safety procedures.", "[Equipment] Equipment Required:", "[Equi... |
88,106 | In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1 | 4 | dx.doi.org/10.17504/protocols.io.ewov1o82olr2/v2 | https://www.protocols.io/view/in-vitro-assembly-of-plasmid-dna-for-direct-clonin-c2aiyace | Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran | TITLE: In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1
AUTHORS: Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran
[DESCRIPTION]
Protocol detailing a Gibson-assembly-based direct cloning method for Lactiplatibacillus plantarum WCFS1.
The last step in this version contai... | ["[MOLECULAR CLONING] VECTOR PCR\n\nAmplify the backbone sequence of interest. Always use a high-fidelity polymerase such as Q5 polymerase. PCR parameters: \n \n - Use 10 ng of DNA as a template.\n - Set the initial denaturation time to 1 min.\n - Set the denaturation time during the cycle... |
86,615 | Isolation of mCherry-LC3B and 97Q-GFP vesicles for LC-MS/MS | 1 | dx.doi.org/10.17504/protocols.io.n2bvj3395lk5/v1 | https://www.protocols.io/view/isolation-of-mcherry-lc3b-and-97q-gfp-vesicles-for-cytxxwpn | Dorothy zhao | TITLE: Isolation of mCherry-LC3B and 97Q-GFP vesicles for LC-MS/MS
AUTHORS: Dorothy zhao
[DESCRIPTION]
Aggregation of proteins containing expanded polyglutamine (polyQ) repeats is the cytopathologic hallmark of a group of dominantly inherited neurodegenerative diseases, including Huntington's disease (HD). Huntingtin ... | ["[Isolation of mCherry-LC3B and 97Q-GFP vesicles for LC-MS/MS] Isolation of mCherry-MAP1LC3B and polyQ-GFP positive autophagic vesicles", "[Isolation of mCherry-LC3B and 97Q-GFP vesicles for LC-MS/MS] Label-free quantitative LC-MS/MS", "[Isolation of mCherry-LC3B and 97Q-GFP vesicles for LC-MS/MS] The vesicle isolatio... |
41,588 | Fluorescent labeling Bacillus mycoides | 4 | dx.doi.org/10.17504/protocols.io.bkuukwww | https://www.protocols.io/view/fluorescent-labeling-bacillus-mycoides-bkuukwww | Andreea S | TITLE: Fluorescent labeling Bacillus mycoides
AUTHORS: Andreea S
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>GFP method is performed in bacteria to localize a desired peptide in the bacteria. In this experiment, we are focusing on the Nlp-14a. In the paper written by </span><span style = "... | ["[Preparation of B. mycoides cells for GFP]\nPrepare the B. mycoides strain aliquotes for electroporation.", "[Preparation of B. mycoides cells for GFP]\nCultivate the bacterial strain overnight in LB broth at and at 180 rpm.\n30 °C", "[Preparation of B. mycoides cells for GFP]\nTransfer of the overnight culture int... |
65,038 | Amicon NMWCO Filter Concentration and Buffer Exchange | 1 | null | https://www.protocols.io/view/amicon-nmwco-filter-concentration-and-buffer-excha-cbrnsm5e | Lauren Adams | TITLE: Amicon NMWCO Filter Concentration and Buffer Exchange
AUTHORS: Lauren Adams
[DESCRIPTION]
This Amicon NMWCO filter protocol helps concentration dilute elution fractions containing protein as well as assists with buffer exchange if necessary.
[STEPS]
1. Wash the filter three times with Optima grade water and ... | ["Wash the filter three times with Optima grade water and centrifuging at 14,000 x g. Equilibrate the column by washing once with the same buffer as the elution buffer containing the target protein for ~10-30 minutes or until the volume has decreased.", "Load the elution fraction containing the target protein into the ... |
66,357 | https://www.facebook.com/ViaKetoGummiesEnPharmacieFrance/ | 4 | dx.doi.org/10.17504/protocols.io.81wgb6e63lpk/v1 | https://www.protocols.io/view/https-www-facebook-com-viaketogummiesenpharmaciefr-cc2vsye6 | Via Keto En Pharmacie France | TITLE: https://www.facebook.com/ViaKetoGummiesEnPharmacieFrance/
AUTHORS: Via Keto En Pharmacie France
[DESCRIPTION]
Via Keto En Pharmacie France
[STEPS]
1. https://www.facebook.com/ViaKetoGummiesEnPharmacieFrance/
https://www.facebook.com/ViaKetoEnPharmacieFrances/
https://www.facebook.com/Via-Keto-Gummies-En-Pharma... | ["https://www.facebook.com/ViaKetoGummiesEnPharmacieFrance/\nhttps://www.facebook.com/ViaKetoEnPharmacieFrances/\nhttps://www.facebook.com/Via-Keto-Gummies-En-Pharmacie-France-101317322656200\nhttps://via-keto-gummies-en-pharmacie-france.jimdosite.com/\nhttps://lexcliq.com/via-keto-en-pharmacie-france-reaction-choquant... |
93,030 | Hutu80 and NCI h716 treatment with SCFAs and α synuclein Western Blotting | 4 | dx.doi.org/10.17504/protocols.io.261ged12dv47/v1 | https://www.protocols.io/view/hutu80-and-nci-h716-treatment-with-scfas-and-synuc-c64ezgte | Chaima Ezzine | TITLE: Hutu80 and NCI h716 treatment with SCFAs and α synuclein Western Blotting
AUTHORS: Chaima Ezzine
[DESCRIPTION]
This protocol describe the treatment of human enteroendocrine cell models Hutu80 and NCI h716 parental cells with SCFAs and GCase inhibitor (CBE) in order to detect the impact of SCFAs and GCAse inhibi... | ["Hutu80 and NCI h716 parental cells were seeded in wells at a density of 1.0 105 cells the day before treatments and incubated at 37 °C in a 5% CO2 atmosphere", "Cells were treated with differents concentrations ( 0.5 ; 1 ; 2 ; 4 and 6 mM) of SCFAs Acetate, propionate and butyrate (100 mM stock solutions in water (SI... |
103,387 | Refractive index adjusted imaging medium: Sorbitol (RI ~ 1.4) - Yeast | 0 | dx.doi.org/10.17504/protocols.io.n2bvjnppwgk5/v1 | https://www.protocols.io/view/refractive-index-adjusted-imaging-medium-sorbitol-dg733zqn | Mathias Hammer, Ammeret Rossouw, Azra Lari, Ben Montpetit, David Grunwald | TITLE: Refractive index adjusted imaging medium: Sorbitol (RI ~ 1.4) - Yeast
AUTHORS: Mathias Hammer, Ammeret Rossouw, Azra Lari, Ben Montpetit, David Grunwald
[DESCRIPTION]
This protocol describes the steps to prepare imaging medium for Saccharomyces cerevisiae with adjusted refractive index. This medium is optimized... | ["[Preparation of 30ml refractive index adjusted imaging medium with Sorbitol] Compound medium for autoclave", "[Preparation of 30ml refractive index adjusted imaging medium with Sorbitol] Fill a 50 ml flask with 3.9 mLddH2O\nAdd a magnetic stirring bar and place the flask on a stirring hot plate.", "[Preparation of 30... |
39,505 | ANY-maze Protocol (Elevated Plus Maze) v6.2 | 1 | null | https://www.protocols.io/view/any-maze-protocol-elevated-plus-maze-v6-2-bitrkem6 | Cristina Corral, Lieselot Carrette, Olivier George | TITLE: ANY-maze Protocol (Elevated Plus Maze) v6.2
AUTHORS: Cristina Corral, Lieselot Carrette, Olivier George
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for running rats in the Elevated Plus Maze with ANY-maze software analysis of the experimental data.</div><div class = "t... | ["Open a \"New Experiment\" from the file tab and name the protocol and modify settings as described below(Or open EPM CC, which has all the settings as recommended below)From the dropdown menu below the protocol title select Video tracking mode to record video tracking From the dropdown menu below the Video tracking m... |
null | null | null | dx.doi.org/10.17504/protocols.io.j9ncr5e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A 4-month-old male wild-type mouse swimming. The water temperature was 34 °C.</p>
[STEPS]
?. | [] |
100,840 | RSV standard and copyback genomes PCR Protocols | 0 | dx.doi.org/10.17504/protocols.io.5qpvokkndl4o/v1 | https://www.protocols.io/view/rsv-standard-and-copyback-genomes-pcr-protocols-deqg3dtw | Carolina Lopez | TITLE: RSV standard and copyback genomes PCR Protocols
AUTHORS: Carolina Lopez
[DESCRIPTION]
Protocol for the detection of the Respiratory Syncytial Virus (RSV) standard and copyback genomes by PCR
[BEFORE_START]
PRINCIPLES BEHIND THE PROCEDURE MUST BE UNDERSTOOD. PLEASE CONSULT WITH EXPERIENCED LAB MEMBER THE FIRST ... | ["[RSV standard and copyback genomes PCR Protocols] *The “Big” DVG design can detect cbVGs with break before 14882 and rejoin before 15112 (RSVA2). The “Small” DVG design can detect cbVGs with break before 15112 and rejoin before 15194 (RSVA2). The “Small” DVG design should theoretically also pick up big DVGs, but bigg... |
65,225 | Cell lysis and gel electrophoresis for protein analysis of HeLa cells | 1 | dx.doi.org/10.17504/protocols.io.j8nlkk1w1l5r/v1 | https://www.protocols.io/view/cell-lysis-and-gel-electrophoresis-for-protein-ana-cbxhspj6 | OLIVIA HARDING, Erika L.F. Holzbaur | TITLE: Cell lysis and gel electrophoresis for protein analysis of HeLa cells
AUTHORS: OLIVIA HARDING, Erika L.F. Holzbaur
[DESCRIPTION]
Here, we present multiple protocols used for biochemical analysis of protein expression and association. First, we used a simple lysis technique to determine the efficiency of an siRN... | ["[Wash cells] Aspirate media from dishes.", "[Wash cells] Wash samples quickly x2 with ice cold PBS.", "[Wash cells] Add 150 µL RIPA + inhibitors to dish and scrape cells into 1.5 uL tube, OR add buffer to thawed sample and resuspend by pipetting.", "[Wash cells] Rotate resuspended sample on end-over-end machine at 4 ... |
67,832 | BASIC PROTOCOL 2: Download MIDAS Reference Database | 5 | dx.doi.org/10.17504/protocols.io.kxygxz6xwv8j/v1 | https://www.protocols.io/view/basic-protocol-2-download-midas-reference-database-cegytbxw | miriam.goldman , chunyu.zhao | TITLE: BASIC PROTOCOL 2: Download MIDAS Reference Database
AUTHORS: miriam.goldman , chunyu.zhao
[DESCRIPTION]
This protocol describes how to download all or part of a MIDASDB, a set of custom files constructed from microbial genome sequences and containing all the information needed to metagenotype the species det... | ["Initialize a local copy of MIDASDB-UHGG", "Customize the MIDASDB download. In Basic Protocol 1, 22 species were present in at least one sample list_of_species.tsv). Now those will be downloaded in the database components (both rep-genome and pan-genome) only for these 22 species.", "The download has completed success... |
38,522 | NEBnext library construction and sequencing for SARS-CoV-2: Adapting COVID-19 ARTIC protocol | 1 | dx.doi.org/10.17504/protocols.io.bhu2j6ye | https://www.protocols.io/view/nebnext-library-construction-and-sequencing-for-sa-bhu2j6ye | Jennifer Giandhari, Sureshnee Pillay, Houriiyah Tegally, Eduan Wilkinson, Benjamin Chimukangara, Richard Lessells, Yunus Moosa, Inbal Gazy, Maryam Fish, Lavanya Singh, Khulekani Sedwell Khanyile, Vagner Fonseca, Marta Giovanetti, Luiz Carols Alcantara, Tulio de Oliveira | TITLE: NEBnext library construction and sequencing for SARS-CoV-2: Adapting COVID-19 ARTIC protocol
AUTHORS: Jennifer Giandhari, Sureshnee Pillay, Houriiyah Tegally, Eduan Wilkinson, Benjamin Chimukangara, Richard Lessells, Yunus Moosa, Inbal Gazy, Maryam Fish, Lavanya Singh, Khulekani Sedwell Khanyile, Vagner Fonseca... | ["[cDNA]\nPrepare the cDNA mastermix in the pre-PCR clean room. The mastermix hood must be decontaminated before and after use with 10% extran, and 70% ethanol, and sterilised with ultraviolet light (UV).", "[cDNA]\nMix the following components in a labeled 1.5ml Component: AB1ComponentVolume (ul)250μM Random Hexamers... |
32,031 | Red Velvet Cupcakes | null | dx.doi.org/10.17504/protocols.io.bbh7ij9n | null | Hannah Gunderman | TITLE: Red Velvet Cupcakes
AUTHORS: Hannah Gunderman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Photograph by Alexandra Kusper on Unsplash.</div><div class = "text-block"><span>Note: this recipe is adapted from </span><a href="https://choosingchia.com/vegan-red-velvet-cupcakes" style = "text-d... | ["Preheat the oven to 350 degrees F and line a cupcake pan with cupcake liners.", "Mix the almond milk and apple cider vinegar and set aside for 5 minutes to turn to “buttermilk.”", "Next, add the coconut oil and vanilla extract.", "Mix all the dry ingredients together in a bowl.", "Pour the wet ingredients into the dr... |
92,554 | Western blot in homogenised mouse brain samples | 1 | dx.doi.org/10.17504/protocols.io.yxmvm26m5g3p/v2 | https://www.protocols.io/view/western-blot-in-homogenised-mouse-brain-samples-c6mizc4e | Katherine Brimblecombe, Natalie Connor-Robson, Stephanie J Cragg | TITLE: Western blot in homogenised mouse brain samples
AUTHORS: Katherine Brimblecombe, Natalie Connor-Robson, Stephanie J Cragg
[DESCRIPTION]
This protocol is for western blot analysis of proteins in homogenised mouse brain samples. The sensitivity and selectively of this assay is dependent on the efficacy of antibo... | ["[Preparing Samples] Take samples out of the -80°C freezer and keep on dry ice until ready to digest.", "[Preparing Samples] On wet ice, add 200 µL RIPA Buffer (see Materials) to each unilateral striatum sample.", "[Preparing Samples] Mix thoroughly until sample completely blended with Tissue Tearor.", "[Preparing Sam... |
88,515 | Cryo-EM Grid preparation | 4 | null | https://www.protocols.io/view/cryo-em-grid-preparation-c2pbydin | Annan SI Cook | TITLE: Cryo-EM Grid preparation
AUTHORS: Annan SI Cook
[DESCRIPTION]
This protocol details Cryo-EM grid preparation.
[STEPS]
SECTION: Grid Preparation
1. Freshly glow-discharge the QUANTIFOIL R 2/1 mesh Cu 300 holey carbon grids using a glow-discharge system (PELCO easiGlow) at 25 mA current for 1 min.
SECTION: Sampl... | ["[Grid Preparation] Freshly glow-discharge the QUANTIFOIL R 2/1 mesh Cu 300 holey carbon grids using a glow-discharge system (PELCO easiGlow) at 25 mA current for 1 min.", "[Sample Application] Mix the PI3KC3-C1~RAB1A sample with 0.05% n-Octyl-β-D-Glucopyranoside (OG) to achieve the desired final concentration.", "[Sa... |
54,341 | Protocol of DNA extraction for Nanopore long-reads genome sequencing | 4 | dx.doi.org/10.17504/protocols.io.bzbdp2i6 | https://www.protocols.io/view/protocol-of-dna-extraction-for-nanopore-long-reads-bzbdp2i6 | Cros-Arteil Sandrine | TITLE: Protocol of DNA extraction for Nanopore long-reads genome sequencing
AUTHORS: Cros-Arteil Sandrine
[DESCRIPTION]
Protocol of DNA extraction for Nanopore long-reads genome sequencing adapted for fungal genomic
[GUIDELINES]
Use only materials and reagents DNase free. Never vortex and store DNA extracts on ice f... | ["Using liquid nitrogen and pre-chilled mortar and pestle, grind 0.2/0.3 g of fresh mycelium into powder, in less than 1 minute. Place the powder into a 15 mL Falcon tube and store in liquid nitrogen or at -80°C.\n\nUse only materials and reagents DNase free. Never vortex and store DNA extracts on ice from step 9. Avoi... |
33,816 | Agarose Gel DNA Extraction (QIAquick) | 1 | null | https://www.protocols.io/view/agarose-gel-dna-extraction-qiaquick-bc9yiz7w | Kenneth Schackart | TITLE: Agarose Gel DNA Extraction (QIAquick)
AUTHORS: Kenneth Schackart
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Extract DNA from agarose gel using Qiagen® QIAquick® Gel Extraction Kit. The kit can be used for centrifuge processing or vacuum processing. The steps given here are only for centr... | ["[Excise Gel]\nLabel and weigh 1 uncolored 1.5 mL centrifuge tube for each gel band to be exracted.", "[Excise Gel]\nUsing a clean razor blade, excise DNA band from gel and place in corresponding 1.5 mL centrifuge tube. Try to cut the gel as close to the DNA band as possible\nIt is critical to avoid exposure of skin o... |
null | null | null | dx.doi.org/10.17504/protocols.io.iegcbbw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Teeth were first disinfected with 75% ethanol and then washed with phosphate-buffered saline. Briefly, PDLSCs were separated from periodontal ligament in the middle one-third of the root. Subsequently, MSCs were digested in a solution of 3 mg/mL collagenase type I (Worthingto... | [] |
72,040 | Analysis of glycosphingolipids from human cerebrospinal fluid | 1 | dx.doi.org/10.17504/protocols.io.5qpvorrndv4o/v1 | https://www.protocols.io/view/analysis-of-glycosphingolipids-from-human-cerebros-cikguctw | David A Priestman, Reuben Bush, Maria Leondaraki, Danielle te Vruchte, Kerri-Lee Wallom, María E Fernández-Suárez, Frances M Platt | TITLE: Analysis of glycosphingolipids from human cerebrospinal fluid
AUTHORS: David A Priestman, Reuben Bush, Maria Leondaraki, Danielle te Vruchte, Kerri-Lee Wallom, María E Fernández-Suárez, Frances M Platt
[DESCRIPTION]
Interest in the role of cellular glycosphingolipids (GSLs) in health and disease led to us devel... | ["[GSL preparation from cerebrospinal fluid] Use 100 µL cerebrospinal fluid for GSL quantification.", "[GSL preparation from cerebrospinal fluid] Add 100 µL de-ionised water to make the volume up to 200 µL in a 1.5 ml screw-cap tube.", "[GSL preparation from cerebrospinal fluid] Add 0.8 mL of chloroform/methanol (1:2, ... |
90,735 | Western blotting for Rubicon and Pacer expression | 4 | dx.doi.org/10.17504/protocols.io.n92ldmq2nl5b/v1 | https://www.protocols.io/view/western-blotting-for-rubicon-and-pacer-expression-c4upywvn | Dan Tudorica | TITLE: Western blotting for Rubicon and Pacer expression
AUTHORS: Dan Tudorica
[DESCRIPTION]
Traditional.
[STEPS]
1. Wash adherent cells to be analyzed twice with PBS, then scrape into microcentrifuge tubes. Pellet cells via tabletop centrifuge, aspirate supernatant, and lyse for 30 minutes on a rocker at 4 C (lysis ... | ["Wash adherent cells to be analyzed twice with PBS, then scrape into microcentrifuge tubes. Pellet cells via tabletop centrifuge, aspirate supernatant, and lyse for 30 minutes on a rocker at 4 C (lysis buffer: 50 mM HEPES 7.4, 150 mM NaCl, 0.5% NP-40 detergent, 1 mM TCEP, cOmplete protease inhibitor tablet).", "Pellet... |
61,597 | Diagnosis of hypertension based on TCM (Traditional Chinese Medicine) constitution and wrist pulse wave signal | 1 | null | https://www.protocols.io/view/diagnosis-of-hypertension-based-on-tcm-traditional-b8d5rs86 | Lin Fan, Zhong-Ming Wang, Rong zhang, Yan Li, Xiao-Kang Zhang, Anonymous | TITLE: Diagnosis of hypertension based on TCM (Traditional Chinese Medicine) constitution and wrist pulse wave signal
AUTHORS: Lin Fan, Zhong-Ming Wang, Rong zhang, Yan Li, Xiao-Kang Zhang, Anonymous
[DESCRIPTION]
In previous studies, pulse signals have been analysed primarily to standardise and objectify pulse dia... | ["Pulse sampler", "Design of pulse sampler", "Proposed pulse preprocessing method \nPulse de-noising \nPeriod segmentation", "Pulse signal feature extract \nTime-domain features \nEnergy features of wavelet packet", "Statistical analysis \nTest of normality. \nLinearity test. \nIndependent sample t-test. \nBinary Logis... |
61,365 | Worm Synchronization Protocol (Bleaching) | 4 | dx.doi.org/10.17504/protocols.io.3byl4b5xovo5/v1 | https://www.protocols.io/view/worm-synchronization-protocol-bleaching-b76vrre6 | Alid Al-Asmar, Gabriel Madirolas, Martina Dal Bello, Tommaso Biancalani, Alfonso Pérez Escudero | TITLE: Worm Synchronization Protocol (Bleaching)
AUTHORS: Alid Al-Asmar, Gabriel Madirolas, Martina Dal Bello, Tommaso Biancalani, Alfonso Pérez Escudero
[DESCRIPTION]
A protocol for synchronizing a large number of C. elegans worms. Worm bodies are decomposed using a mixture of bleach and sodium hydroxide, for a shor... | ["Wash one1 or several2 worm plates3 with milli-Q water4. Put the worms into a 1.7 mL Eppendorf tube5.\n\n1 To wash a plate: Remove a small chunk of agar near the plate border (we use a metal spatula). Using the 1 mL pipette, add 1-2 mL of milli-Q water to the plate. Tilt the plate so that all the water is on the missi... |
null | null | null | dx.doi.org/10.17504/protocols.io.useewbe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We... | ["[Prepare Primer Mix (Optional, only for multiplexed Primer ID library prep)] {\"blocks\":[{\"key\":\"7r2b5\",\"text\":\"For multiplexing sequencing, first, prepare Primer Mix.\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[{\"offset\":0,\"length\":55,\"style\":\"bold\"}],\"entityRanges\":[],\"data\":[]},{... |
37,406 | MERS-CoV Spike Glycoprotein (GP) – ELISA Jenner Clinical SOP Template | 1 | dx.doi.org/10.17504/protocols.io.bgr6jv9e | https://www.protocols.io/view/mers-cov-spike-glycoprotein-gp-elisa-jenner-clinic-bgr6jv9e | Mustapha Bittaye, Sarah Gilbert, Katie Ewer, Teresa Lambe | TITLE: MERS-CoV Spike Glycoprotein (GP) – ELISA Jenner Clinical SOP Template
AUTHORS: Mustapha Bittaye, Sarah Gilbert, Katie Ewer, Teresa Lambe
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">This Standard Operating Procedure (SOP) describes the tech... | ["[Day 1 – Coating ELISA plates on the bench]\nPrint off a new ELISA record sheet for each experiment (accessed from: X:\\KEwer\\7. ELISA\\Templates and protocols).", "[Day 1 – Coating ELISA plates on the bench]\nNumber the experiment with the next experiment number and fill this in with the required information throug... |
83,462 | 2-step PCR mixture and conditions (Barcoded-head primers for seqs pooling) | 4 | dx.doi.org/10.17504/protocols.io.e6nvwdp32lmk/v1 | https://www.protocols.click/view/2-step-pcr-mixture-and-conditions-barcoded-head-pr-cvrew53e | Yin-Tse Huang, Tsu-Chun Hung | TITLE: 2-step PCR mixture and conditions (Barcoded-head primers for seqs pooling)
AUTHORS: Yin-Tse Huang, Tsu-Chun Hung
[DESCRIPTION]
PCR mixture and condition (PowerPol 2X PCR Mix)
[STEPS]
1. Wear glove, clean up the working bench w. 1% bleach
SECTION: For 1' PCR head-primers
2. Prepare 1' PCR master mixutre fo... | ["Wear glove, clean up the working bench w. 1% bleach", "[For 1' PCR head-primers] Prepare 1' PCR master mixutre for head-primers (prepare 1.2X of solutions for pipetting error if needed)\n \nPCR mixture for head-primers for each reaction", "[For 1' PCR head-primers] Mix the 1' PCR master mixture gently by pi... |
106,357 | RNAscope for FFPE Mouse Tissue | 0 | dx.doi.org/10.17504/protocols.io.81wgbzwkqgpk/v1 | https://www.protocols.io/view/rnascope-for-ffpe-mouse-tissue-dj4v4qw6 | Hector Martell Martinez | TITLE: RNAscope for FFPE Mouse Tissue
AUTHORS: Hector Martell Martinez
[DESCRIPTION]
This protocol details the RNAscope for FFPE Mouse Tissue.
[STEPS]
SECTION: DAY 1 - Bake/Adhere
1. Spray RNase away on bench top, slide holder, metal container.
SECTION: DAY 1 - Bake/Adhere
2. Warm oven and slide holder up to 60 °C.
... | ["[DAY 1 - Bake/Adhere] Spray RNase away on bench top, slide holder, metal container.", "[DAY 1 - Bake/Adhere] Warm oven and slide holder up to 60 °C.", "[DAY 1 - Bake/Adhere] Label the lower part of the slide with a pencil.\n\nCan place slides on bench (since sprayed with RNase away).", "[DAY 1 - Bake/Adhere] Put slid... |
null | null | null | dx.doi.org/10.17504/protocols.io.e5hbg36 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>SpinOUT™ protocol for buffer exchange of peptide and protein samples.</p>
<p>(Cat. # 786-170 to 786-173, 786-703 to 786-708, 786-865 to 786-869)</p>
[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p>The SpinOUT™ columns are versatile, spin-format columns for the desalting... | [] |
83,942 | Anxa1 Immunostaining | 1 | dx.doi.org/10.17504/protocols.io.36wgq391ylk5/v1 | https://www.protocols.click/view/anxa1-immunostaining-cv8ew9te | connor.davidson | TITLE: Anxa1 Immunostaining
AUTHORS: connor.davidson
[DESCRIPTION]
Protocol for perfusion, slicing, and immunostaining for Anxa1 in the striatum.
[STEPS]
SECTION: Perfusion
1. Anesthetize mouse in an anesthesia chamber set to 5% isoflurane, until breathing slows and foot pinch reflex stops.
SECTION: Perfusion
2. Rest... | ["[Perfusion] Anesthetize mouse in an anesthesia chamber set to 5% isoflurane, until breathing slows and foot pinch reflex stops.", "[Perfusion] Restrain the anesthetized mouse with tape such that the chest is facing upwards, and all limbs are secured.", "[Perfusion] Using surgical scissors, cut the skin away to reveal... |
null | null | null | dx.doi.org/10.17504/protocols.io.pw6dphe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>In order to increase the number of viral particles a blind passage of sera might be performed in <em>Aedes albopictus C6/36</em> cells.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
94,454 | Parallel Beam test for mice | 4 | dx.doi.org/10.17504/protocols.io.261gedbqjv47/v1 | https://www.protocols.io/view/parallel-beam-test-for-mice-c8gwztxe | Cristian González-Cabrera, Katharina Draggendorf, Matthias Prigge | TITLE: Parallel Beam test for mice
AUTHORS: Cristian González-Cabrera, Katharina Draggendorf, Matthias Prigge
[DESCRIPTION]
The Parallel Beam Test Protocol is designed to evaluate the motor coordination of mice. The test involves a 150 cm long balance beam set at a height of 50-60 cm, with safety measures to catch fal... | ["[Objective] This protocol allows for the evaluation of balance and coordination in mice, which can be crucial for studies involving neurological conditions, muscular diseases, or the effects of drugs on motor functions", "[Apparatus] A 150 cm long balance beam placed on two opposing supports.\nThe height between the ... |
83,955 | Basic maintenance protocol for human induced pluripotent stem cell (hIPSCs) | 4 | dx.doi.org/10.17504/protocols.io.81wgbx1q1lpk/v2 | https://www.protocols.click/view/basic-maintenance-protocol-for-human-induced-pluri-cv8tw9wn | William J Buchser, Mallory Wright, purva patel, jwaligor, ckremitz | TITLE: Basic maintenance protocol for human induced pluripotent stem cell (hIPSCs)
AUTHORS: William J Buchser, Mallory Wright, purva patel, jwaligor, ckremitz
[DESCRIPTION]
Human Induced pluripotent stem cell (hIPSCs) maintenance protocol. This includes thawing, passaging, performing a media change, single cell dissoc... | ["[Thawing iPSCs] Quickly thaw the frozen vial in a 37 °C water bath 2 min", "[Thawing iPSCs] Using a 10 ml serological pipette, add 9 mL of media into 15 ml conical tube.", "[Thawing iPSCs] Add the contents of the cryovial into the 15 ml conical tube with a 5 ml serological pipette", "[Thawing iPSCs] Spin down conical... |
80,850 | Small Business Moorea | 1 | dx.doi.org/10.17504/protocols.io.rm7vzbpkrvx1/v1 | https://www.protocols.io/view/small-business-moorea-cs7swhne | Clairey Yang | TITLE: Small Business Moorea
AUTHORS: Clairey Yang
[DESCRIPTION]
Sustainability is a hot topic in French Polynesia. For example, the South Pacific Tourism Organization launched a Sustainable Tourism Policy Framework with goals extending to 2030. The Blue Climate Initiative, an ocean-centered environmental program, add... | ["[Defining a Small Business] This project defines a small business as any business that is not part of a larger conglomerate.", "[Defining Industry Type] Outreach will be focused on only food businesses in Moorea. This includes restaurants, snack shacks, grocery stores, food trucks, food producers, and roadside stands... |
46,359 | sciMAP-ATAC | 1 | dx.doi.org/10.17504/protocols.io.brhxm37n | https://www.protocols.io/view/scimap-atac-brhxm37n | Andrew Adey, Casey Thornton | TITLE: sciMAP-ATAC
AUTHORS: Andrew Adey, Casey Thornton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">High-throughput single cell genomic assays resolve the heterogeneity of cell states in complex tissues, however, the spatial orientation within the network of interconnected cells is lost. As cell... | ["[Prepare Nuclei Isolation Buffer]\nConstruct 50mL Nuclei Isolation Buffer (NIB): ABC1Final\nConcentrationStock\nConcentrationVolume of Stock210 mM Tris HCl, pH 7.51M\nTris-HCl, pH7.5500 uL310 mM NaCl5M\nNaCl100 uL43mM MgCl21M\nMgCl2150 uL50.1 % Igepal10%\nIgepal500 uL60.1 % Tween10%\nTween500 uL7ddH20to 50mL (add 48... |
null | null | null | dx.doi.org/10.17504/protocols.io.ci6uhd | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
How to preform a trypsin digestion
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
34,087 | CODEX Acquisition Protocol | null | dx.doi.org/10.17504/protocols.io.bdifi4bn | null | Franchesca Farris, Marda Jorgensen, Jerelyn Nick, Jesus Peñaloza | TITLE: CODEX Acquisition Protocol
AUTHORS: Franchesca Farris, Marda Jorgensen, Jerelyn Nick, Jesus Peñaloza
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"> Multiplex imaging of lymph node, thymus and spleen is accomplished using the Akoya Biosciences CODEX system at the HubMAP Tissue Mapping Cente... | ["[loading a blank coverslip and priming the instrument]\n:Untwist the two wing knobs on the coverslip stage to remove the lid holding the fluidic line", "[loading a blank coverslip and priming the instrument]\nIf running 2 experiments back to back, first remove old cover slip, using bent angle forceps. Rinse area well... |
null | null | null | dx.doi.org/10.17504/protocols.io.n7gdhjw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is a very handy, previously published [Ref 1 and 2], rhinovirus (RV) screening and genotyping assay which I and many others have used on many sample extracts, mostly originating from acutely ill paediatric patients, spanning well over a decade's worth of collection dates... | [] |
91,713 | Perturb-seq characterizing regulators of T cell function | 4 | null | https://www.protocols.io/view/perturb-seq-characterizing-regulators-of-t-cell-fu-c5s9y6h6 | Andrea R Daniel | TITLE: Perturb-seq characterizing regulators of T cell function
AUTHORS: Andrea R Daniel
[DESCRIPTION]
This protocol describes methods for a Perturb-seq assay characterizing transcriptional regulators of T cell function.
[STEPS]
SECTION: gRNA library cloning
1. Oligonucleotide pools containing 40-gRNA sequences and ... | ["[gRNA library cloning] Oligonucleotide pools containing 40-gRNA sequences and constant regions for polymerase chain reaction (PCR) amplification were synthesized by Twist Bioscience.", "[gRNA library cloning] gRNA amplicons were gel extracted, PCR purified and input into 20 μl Gibson reactions (5:1 molar ratio of ins... |
null | null | null | dx.doi.org/10.17504/protocols.io.dje4jd | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
[STEPS]
?.
?.
?. | [] |
48,178 | MojoSort™ Human Pan DC Isolation Kit Protocol | 4 | null | https://www.protocols.io/view/mojosort-human-pan-dc-isolation-kit-protocol-btasniee | Ken Lau | TITLE: MojoSort™ Human Pan DC Isolation Kit Protocol
AUTHORS: Ken Lau
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol covers usage of BioLegend's MojoSort™ Human Pan DC Isolation Kit Protocol.</div></div>
[STEPS]
?. Prepare cells from your tissue of interest or blood without lysing er... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4 mL in a 5 mL (12 x 75 mm) polypropylene tube.Note: Keep MojoSort™ Buffer on ice throughout the procedure.", "Filter the cells with... |
44,521 | Generation of Combinatorial CRISPR Libraries | 1 | dx.doi.org/10.17504/protocols.io.bpqhmmt6 | https://www.protocols.io/view/generation-of-combinatorial-crispr-libraries-bpqhmmt6 | David Adams, Nicky Thompson. nt4@sanger.ac.uk. | TITLE: Generation of Combinatorial CRISPR Libraries
AUTHORS: David Adams, Nicky Thompson. nt4@sanger.ac.uk.
[STEPS]
?. [Summary]
This protocol is a step by step protocol based on the method originally described by: Vidigal, J.A. & Ventura, A. Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 librarie... | ["[Summary]\nThis protocol is a step by step protocol based on the method originally described by: Vidigal, J.A. & Ventura, A. Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Nature Communications6, 8083 (2015). Throughout: All PCR clean-ups done with Monarch PCR & DNA cleanup kithttps://w... |
31,484 | Mulheres na praça, saúde de graça: relato de experiência da equipe multiprofissional do grupo de mulheres do bairro Tamarindo | null | dx.doi.org/10.17504/protocols.io.bay4ifyw | null | Elis Sales Muniz Lima, Ana Cesarina Silva Gomes, Hyara Brena Oliveira Rufino, Jamile Xavier de Oliveira | TITLE: Mulheres na praça, saúde de graça: relato de experiência da equipe multiprofissional do grupo de mulheres do bairro Tamarindo
AUTHORS: Elis Sales Muniz Lima, Ana Cesarina Silva Gomes, Hyara Brena Oliveira Rufino, Jamile Xavier de Oliveira
[STEPS] | [] |
62,421 | ViaKeto BHB Apple Gummies Reviews - Can I lose 25kg in 5 Weeks? Fantastic Way to Improve Your Health! | 3 | dx.doi.org/10.17504/protocols.io.ewov1n5dpgr2/v1 | https://www.protocols.io/view/viaketo-bhb-apple-gummies-reviews-can-i-lose-25kg-b87vrzn6 | ViaKeto BHB Apple Gummies | TITLE: ViaKeto BHB Apple Gummies Reviews - Can I lose 25kg in 5 Weeks? Fantastic Way to Improve Your Health!
AUTHORS: ViaKeto BHB Apple Gummies
[DESCRIPTION]
ViaKeto BHB Apple Gummies
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gvxbw7n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>These protocols accompany the following <em>GigaScience</em> publication:</p>
<p> </p>
<p>Benjamin Istace, et al. (2017) De novo assembly and population genomic survey of natural yeast isolates with the Oxford Nanopore MinION sequencer. <em>GigaScience...</em></p>
[STEPS] | [] |
28,284 | MojoSort™ Mouse CD326 (Ep-CAM) Selection Kits Column Protocol | null | dx.doi.org/10.17504/protocols.io.7u4hnyw | null | Sam Li | TITLE: MojoSort™ Mouse CD326 (Ep-CAM) Selection Kits Column Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4mL in a 5 mL (12 x 75 mm) polypropylene tube.Note: Keep MojoSort™ Buffer on ice throughout the procedure.", "Filter the cells with ... |
38,922 | 4: User-friendly protocol: Cost-efficient Primer Exchange Reaction (PER) concatemerization (SABER-FISH) | 1 | null | https://www.protocols.io/view/4-user-friendly-protocol-cost-efficient-primer-exc-bh9ij94e | Jocelyn Y. Kishi, Sylvain W. Lapan, Brian J Beliveau, Emma R. West, Allen Zhu, Hiroshi M. Sasaki, Sinem Saka, Yu Wang, Constance L Cepko, Peng Yin | TITLE: 4: User-friendly protocol: Cost-efficient Primer Exchange Reaction (PER) concatemerization (SABER-FISH)
AUTHORS: Jocelyn Y. Kishi, Sylvain W. Lapan, Brian J Beliveau, Emma R. West, Allen Zhu, Hiroshi M. Sasaki, Sinem Saka, Yu Wang, Constance L Cepko, Peng Yin
[DESCRIPTION]
<div class = "text-blocks"><div class ... | ["[PER mix and extension]\nAdd mix to strip tube with and mix.\n[5µM hairpin]", "[PER mix and extension]\nIncubate for at , then pause cycler.\n37 °C", "[PER mix and extension]\nRemove tube from the cycler, add and mix.\n[10µM probe oligos]", "[PER mix and extension]\nIncubate at for desired extension time (see Not... |
23,814 | Peptide Desalting with a Vacuum Manifold | null | dx.doi.org/10.17504/protocols.io.3hegj3e | null | Rebecca E. Hardman, Jeffrey Lewis, Tara Stuecker | TITLE: Peptide Desalting with a Vacuum Manifold
AUTHORS: Rebecca E. Hardman, Jeffrey Lewis, Tara Stuecker
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Desalting of peptides is an essential step for mass spectrometry workflows. We detail the steps for medium-throughput desalting (10-20 samples) wi... | ["[Buffers]\nPrepare buffers: a. 0.1% TFA – Make 15 ml per sample plus 1-2 ml extra. i. For example: if you are desalting 10 samples, you would make 152 ml (1 5 ml x 10 samples = 150 ml plus 2 ml extra = 152 ml) b. 70% Acetonitrile – Make 2 ml per sample plus 1-2 ml extra.", "[P... |
69,879 | MagAttract + Metapolyzyme metagenomic gDNA extraction from skin swabs | 4 | null | https://www.protocols.io/view/magattract-metapolyzyme-metagenomic-gdna-extractio-cggxttxn | Natalie Ring | TITLE: MagAttract + Metapolyzyme metagenomic gDNA extraction from skin swabs
AUTHORS: Natalie Ring
[DESCRIPTION]
A protocol for the metagenomic extraction of bacterial DNA from skin swab samples (optimised using canine swabs), for use in a rapid diagnostics pipeline. At the end of the protocol, the DNA is cleaned up a... | ["[Extended pre-lysis spin down] Pellet 2x 1.5 ml aliquots of cell-PBS solution in 1.5 ml tubes by centrifuging at maximum speed (13,000 RPM) for 20 minutes, then discard supernatant\n\n3 mL \n\n\n16,000 x g, 20 min, RT Room temperature", "[Metapolyzyme & Proteinase K Lysis] Resuspend cell pellets (which might be i... |
35,355 | Maintenance of TissueCyte System | null | dx.doi.org/10.17504/protocols.io.ber3jd8n | null | Allen Institute for Brain Science | TITLE: Maintenance of TissueCyte System
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocols provides detailed instructions for procedures needed for maintenance of the TissueCyte scanning systems.</div><div class = "text-block"><span style = "fon... | [] |
54,181 | Experiment 4 | 1 | null | https://www.protocols.io/view/experiment-4-by6dpza6 | Dikla Perez , Yael Steinhart , Amir Grinstein , Meike Morren | TITLE: Experiment 4
AUTHORS: Dikla Perez , Yael Steinhart , Amir Grinstein , Meike Morren
[DESCRIPTION]
This experiment provides evidence for this effect in a field setting. We approached residents of a specific city (referred to in what follows as “Smallville”) and offered them the opportunity to make two sequential... | ["Experimental design:\n We have employed two expected visibility conditions (low vs. high) in a between-subjects design.", "Sample:\nn = 50 shoppers (34 women) in a local supermarket in the city of “Smallville.” \nParticipants were randomly assigned to two conditions, in a between-subjects design: high or low expected... |
43,650 | DNA extraction from pinned specimens | 3 | dx.doi.org/10.17504/protocols.io.bnvame2e | https://www.protocols.io/view/dna-extraction-from-pinned-specimens-bnvame2e | Caroline Storer, Demian F Gomez | TITLE: DNA extraction from pinned specimens
AUTHORS: Caroline Storer, Demian F Gomez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The purpose of this protocol is to extract DNA from pinned beetles.</div><div class = "text-block"><span>This protocol is part of the Bark Beetle Mycobiome (BBM) Resea... | [] |
102,109 | Mycrowave synthesis of low molecular weigth deacylated chitosan | 6 | dx.doi.org/10.17504/protocols.io.x54v9d6w4g3e/v2 | https://www.protocols.io/view/mycrowave-synthesis-of-low-molecular-weigth-deacyl-dfx53pq6 | Maria J Torres, Eric S McLamore, Geisianny AM Moreira | TITLE: Mycrowave synthesis of low molecular weigth deacylated chitosan
AUTHORS: Maria J Torres, Eric S McLamore, Geisianny AM Moreira
[DESCRIPTION]
This protocol describes the synthesis of low molecular weight deacylated chitosan using microwave irradiation. The process requires approximately 2.5 hours (including base... | ["[SECTION 1) Materials and solution preparation] Prepare reaction vials\n\nCheck the vial cap and Teflon liner to make sure neither is damageddamage to the cap and both are clean (Fig 1A-B)", "[SECTION 1) Materials and solution preparation] Add solution to the reaction vial.\n\nPipette 8000 µL of 1% deacylated chitosa... |
63,417 | Optima brain Mind Max Pills For Brain Boost 2022 | 1 | dx.doi.org/10.17504/protocols.io.3byl4bd7zvo5/v1 | https://www.protocols.io/view/optima-brain-mind-max-pills-for-brain-boost-2022-b96zr9f6 | Brain and Rain | TITLE: Optima brain Mind Max Pills For Brain Boost 2022
AUTHORS: Brain and Rain
[DESCRIPTION]
Optima brain Mind Max Pills For Brain Boost 2022
[STEPS]
1. Optima brain Mind Max Pills For Brain Boost 2022
In the period of so numerous distractions for all age groups, everyone needs commodity to help them in fastening ... | ["Optima brain Mind Max Pills For Brain Boost 2022\nIn the period of so numerous distractions for all age groups, everyone needs commodity to help them in fastening on their work and being mentally healthy. You need to suppose about so numerous prospects these days before starting a single task due to the technological... |
91,756 | Lifeplan Camera Trapping Protocol | 1 | dx.doi.org/10.17504/protocols.io.q26g7pxp1gwz/v3 | https://www.protocols.io/view/lifeplan-camera-trapping-protocol-c5uky6uw | Hanna M.K. Rogers, Gaia Giedre Banelyte, Arielle M Farrell, Bess Hardwick, Tommi Mononen, Deirdre Kerdraon | TITLE: Lifeplan Camera Trapping Protocol
AUTHORS: Hanna M.K. Rogers, Gaia Giedre Banelyte, Arielle M Farrell, Bess Hardwick, Tommi Mononen, Deirdre Kerdraon
[DESCRIPTION]
Lifeplan is a global biodiversity monitoring project with the aim of assessing the current state of biodiversity worldwide, and using this knowledge... | ["[Preparations]", "[Preparations] Preparing equipment\n\n\n•\tLabel cameras with QR codes on the inside. Choose stickers marked “urban” or “natural” depending on which place you are sampling in the first year.\n\n•\tLabel SD cards with QR codes\n\n\n•\tCheck camera ID and change the Night Mode setting:\n\no\tInsert 6 ... |
null | null | null | dx.doi.org/10.17504/protocols.io.uu9ewz6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Caenorhabditis elegans is an excellent model to study animal chemotaxis and thermotaxis behaviours. These nematodes have highly predictable behaviour pattern towards olfactory cues. A complex chemosensory information processing, based on both temporal and spacial cues, is involv... | ["[Setting up the camera] The skeleton of this system is a burette stand and clamps to hold various parts (Fig.1a). For video capturing we used Dino-Lite digital microscope (Model no AM4113T-GFBW) with USB connector and its software module Dinocapure2.0 (Fig.1b) to record the data. Dino-Lite digital microscope was fixe... |
86,809 | CAMbank: SST Field Processing v1 | 4 | dx.doi.org/10.17504/protocols.io.81wgbxxo1lpk/v1 | https://www.protocols.io/view/cambank-sst-field-processing-v1-cyzzxx76 | Eliah G Overbey, Krista A Ryon, JangKeun Kim, Christopher E Mason | TITLE: CAMbank: SST Field Processing v1
AUTHORS: Eliah G Overbey, Krista A Ryon, JangKeun Kim, Christopher E Mason
[DESCRIPTION]
Field processing of SST vacutainers for the Cornell Aerospace Medicine Biobank (CAMbank).
Instructions for preserving: Serum and RBC Pellets.
[STEPS]
SECTION: Perform Venipuncture
1. After... | ["[Perform Venipuncture] After venipuncture, invert the tubes gently 8 to 10 times to fully mix tube anticoagulant with blood sample.\n\nStore the tube upright at room temperature until centrifugation.\n\nAllow the blood to clot in an upright position for at least 30 minutes. \nFor optimal biochemical results, centrifu... |
88,599 | Sniffles2 methods | 5 | dx.doi.org/10.17504/protocols.io.14egn34qyl5d/v1 | https://www.protocols.io/view/sniffles2-methods-c2rxyd7n | Moritz Smolka, Luis F Paulin, Fritz Sedlazeck | TITLE: Sniffles2 methods
AUTHORS: Moritz Smolka, Luis F Paulin, Fritz Sedlazeck
[DESCRIPTION]
Long-read Structural Variation (SV) calling remains a challenging but highly accurate way to identify complex genomic alterations. Here, we present Sniffles2, which is faster and more accurate than state-of-the-art SV caller ... | ["[Sniffles2 methodology] Installation", "[Sniffles2 methodology] Sniffles single sample SV calling", "[Sniffles2 methodology] Sniffles population SV calling", "[Benchmarking methodology] HG002 SV calling for the following technologies and coverage:\nOxford Nanopore Technologies: 5x, 10x, 20x, 30x and 50x\nPacBio HiFi:... |
76,258 | Culturing of infective agents from infected wheat leaves | 4 | null | https://www.protocols.io/view/culturing-of-infective-agents-from-infected-wheat-cnqavdse | Benjamin Schwessinger, Erin Hill, Peter Solomon | TITLE: Culturing of infective agents from infected wheat leaves
AUTHORS: Benjamin Schwessinger, Erin Hill, Peter Solomon
[DESCRIPTION]
This protocols is part of the ANU Biosecurity mini-research project #1 "Plant Pathogen Diagnostics: Visuals, subcultures, and genomics".
You will be provided four pots of 3-4 week ol... | ["[Week 2: Selection of representative leaf samples, surface sterilisation, and initial culturing.] Carefully, study the plants in each pot and select two leaves with symptoms of infected plants (if possible) and control leaves of uninfected plants. Select two leaves for each treatment group.", "[Week 2: Selection of r... |
84,950 | T cell depletion | 4 | dx.doi.org/10.17504/protocols.io.rm7vzx372gx1/v1 | https://www.protocols.click/view/t-cell-depletion-cw7wxhpe | Connor Monahan | TITLE: T cell depletion
AUTHORS: Connor Monahan
[DESCRIPTION]
This protocol details T cell depletion by antibodies targeting CD4 and CD8.
[STEPS]
SECTION: Procedure
1. Inject mouse intraperitoneally with 250 µg of Ultra-LEAF Purified anti-mouse CD4 or Rat IgG2b isotype control antibodies (Biolegend, Cat #100457 and C... | ["[Procedure] Inject mouse intraperitoneally with 250 µg of Ultra-LEAF Purified anti-mouse CD4 or Rat IgG2b isotype control antibodies (Biolegend, Cat #100457 and Cat #400644; San Diego, CA) 3 and 1 days prior to immunization.", "[Procedure] After immunization, inject mice with 250 µg of antibody weekly.", "[Procedure]... |
86,448 | GRAFTING METHOD FOR ESTIMATING GENOTYPIC DIVERSITY IN ACROPORA CERVICORNIS | 4 | dx.doi.org/10.17504/protocols.io.bp2l6xxnklqe/v1 | https://www.protocols.io/view/grafting-method-for-estimating-genotypic-diversity-cynqxvdw | Macarena Blanco-Pimentel, Johanna Calle-Triviño, Megan K. Morikawa | TITLE: GRAFTING METHOD FOR ESTIMATING GENOTYPIC DIVERSITY IN ACROPORA CERVICORNIS
AUTHORS: Macarena Blanco-Pimentel, Johanna Calle-Triviño, Megan K. Morikawa
[DESCRIPTION]
The grafting method has been proposed as a potential tool to differentiate corals that share the same genotype from those with different genotypes... | ["[DEFINE OBJECTIVES OF THE STUDY] The overarching goal of this method is to establish who are the unique genets and who the clonal ones among a set of ramets of unknown genetic nature. That is to say, to unveil genotypic diversity. This in turn will ideally have the objective of ensuring or increasing the genetic dive... |
null | null | null | dx.doi.org/10.17504/protocols.io.hmab42e | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
93,731 | Extraction of sediment samples using a modified Bligh and Dyer method: Hinrichs Lab | 6 | null | https://www.protocols.io/view/extraction-of-sediment-samples-using-a-modified-bl-c7sbznan | Christopher CK Klaembt, Lennart Stock LS | TITLE: Extraction of sediment samples using a modified Bligh and Dyer method: Hinrichs Lab
AUTHORS: Christopher CK Klaembt, Lennart Stock LS
[DESCRIPTION]
This updated Bligh and Dyer method is based on Xavier Prieto's protocol "OrgGeochem - Met007.1- Extraction of sediment samples using the Bligh and Dyer method" (rev... | ["[Freeze drying of sediments] Take your samples out of the freezer", "[Freeze drying of sediments] Cover the container with aluminum foil", "[Freeze drying of sediments] Poke little holes in the foil (syringe needles/ Pasteur pipettes work best)", "[Freeze drying of sediments] Follow freeze dryer instructions you find... |
58,196 | Amplification of cytb gene of Plasmodium spp. in blood spots from wild animals | 2 | dx.doi.org/10.17504/protocols.io.bp2l619k5vqe/v1 | https://www.protocols.io/view/amplification-of-cytb-gene-of-plasmodium-spp-in-bl-b43uqynw | Gabriela Ulloa Urizar | TITLE: Amplification of cytb gene of Plasmodium spp. in blood spots from wild animals
AUTHORS: Gabriela Ulloa Urizar
[DESCRIPTION]
Amplification of the Plasmodium mitochondrial cytochrome oxidase b gene (cytb) for detection of the Plasmodium genus in wildlife samples.
[BEFORE_START]
Materials
DW2 and DW4 primers
Plat... | [] |
28,963 | Miniprep Protocol (QIAGEN) | null | dx.doi.org/10.17504/protocols.io.8ibhuan | null | Aditya Mohan | TITLE: Miniprep Protocol (QIAGEN)
AUTHORS: Aditya Mohan
[STEPS]
?. Resuspend pelleted bacterial cells in 250 μl Buffer P1 and transfer to a microcentrifuge tube.
?. Add 250 μl Buffer P2 and mix thoroughly by inverting the tube 4–6 times.
?. Mix gently by inverting the tube. Do not vortex, as this will result in sheari... | ["Resuspend pelleted bacterial cells in 250 μl Buffer P1 and transfer to a microcentrifuge tube.", "Add 250 μl Buffer P2 and mix thoroughly by inverting the tube 4–6 times.", "Mix gently by inverting the tube. Do not vortex, as this will result in shearing of genomic DNA.", "Add 350 μl Buffer N3 and mix immediately and... |
47,408 | PAXgene Processing by RNA Extraction | 4 | dx.doi.org/10.17504/protocols.io.kxygxpnywl8j/v1 | https://www.protocols.io/view/paxgene-processing-by-rna-extraction-bsiqncdw | Clemens Scherzer, Bradley Hyman, Charles Jennings | TITLE: PAXgene Processing by RNA Extraction
AUTHORS: Clemens Scherzer, Bradley Hyman, Charles Jennings
[DESCRIPTION]
This protocol explains the Standard Operating Protocol for performing Paxgene Processing by RNA extraction.
[BEFORE_START]
***NOTE: Please see Appendix in guidelines for miRNA Extraction Protocol. MiRN... | ["[PAXgene Processing BY RNA Extraction] Place all 2 PAXgene tubes in the 4 °C fridge from blood draw if RNA extraction is NOT to be done the next day.", "[PAXgene Processing BY RNA Extraction] Incubate PAXgene Blood RNA Tubes for 1440 min at 4 Room temperature (25°C) after blood collection or removal from 4°C before p... |
null | null | null | dx.doi.org/10.17504/protocols.io.juicnue | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Yeilds 20 mL of Lysis buffer to be used in Phenol/ chloroform genomic DNA extrations.</p>
[STEPS]
?.
?.
?. | [] |
15,452 | Angiotensin-converting enzyme inhibitory assay | null | dx.doi.org/10.17504/protocols.io.tb4eiqw | null | Francisco Herrera | TITLE: Angiotensin-converting enzyme inhibitory assay
AUTHORS: Francisco Herrera
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The use of extracts obtained from various plants is an activity developed for years with the purpose of taking advantage of resources from the regions to prevent or cure a... | ["Aqueous extract (40 µL) was added to a 20 µL solution of ACE (Sigma) (100 mU/mL), and incubated at 37 °C for 5 min.", "Subsequently, 100 µL of HHL prepared at a concentration of 0.3% in a mixture of 40 µmol potassium phosphate buffer (JT Baker, Mexico)", "Later 300 µM sodium chloride buffer (JT Baker, Center Valley, ... |
40,825 | Direct ELISA for investigating the binding of chemically-made Protein-LAG to immunoglobulins. | 6 | dx.doi.org/10.17504/protocols.io.bj4zkqx6 | https://www.protocols.io/view/direct-elisa-for-investigating-the-binding-of-che-bj4zkqx6 | Angel Justiz-Vaillant | TITLE: Direct ELISA for investigating the binding of chemically-made Protein-LAG to immunoglobulins.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protein LAG (SpLAG) is an immunoglobulin-binding protein that interacts with the Fc and Fab regions of many mamma... | ["This ELISA is used to study the interaction of protein-LAG (SpLAG) with diverse immunoglobulins.", "The 96 well microtitre plate is coated overnight at 4°C with 1 µg/µl per well of purified immunoglobulins or 50 µl of any animal sera in carbonate-bicarbonate buffer pH 9.6.", "Then plate is treated with bovine serum a... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.