id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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45,132 | Laurel Wilt / Raffaelea lauricola screening from wood pieces with QIAGEN – QIAamp Fast DNA Stool Mini Kit | 3 | dx.doi.org/10.17504/protocols.io.bqbkmskw | https://www.protocols.io/view/laurel-wilt-raffaelea-lauricola-screening-from-woo-bqbkmskw | Romina Gazis-Seregina, Pedro Pablo Parra | TITLE: Laurel Wilt / Raffaelea lauricola screening from wood pieces with QIAGEN – QIAamp Fast DNA Stool Mini Kit
AUTHORS: Romina Gazis-Seregina, Pedro Pablo Parra
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes DNA extraction of wood samples for the detection of R. lauricola.... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.jxicpke | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol was developed with the aim of sequencing the GC rich part of heterogeneous genomes with the Illumina technology. To this aim, fragmented genomic DNA is denaturized up to 90°C before library preparation. This procedure enriches the library in GC-rich fragments an... | [] |
62,219 | Monitoring Citrobacter rodentium infection dynamics in vivo using biophotonic imaging | 4 | dx.doi.org/10.17504/protocols.io.4r3l2oem3v1y/v1 | https://www.protocols.io/view/monitoring-citrobacter-rodentium-infection-dynamic-b8zjrx4n | Hannah Read, Priyali Patel, Siouxsie Wiles | TITLE: Monitoring Citrobacter rodentium infection dynamics in vivo using biophotonic imaging
AUTHORS: Hannah Read, Priyali Patel, Siouxsie Wiles
[DESCRIPTION]
Citrobacter rodentium is a Gram-negative bacterium which infects laboratory mice in a similar way to how enteropathogenic Escherichia coli (EPEC) and enterohae... | ["Place mice into the anaesthetic induction chamber.", "Induce anaesthesia using a flow rate of 1 L/min oxygen combined with 5% isoflurane.", "When the animals have lost their righting reflex, remove them from the induction chamber and placed them on their backs within the imaging chamber. Ensure each animal’s nose is ... |
48,161 | Daughter of Frankenstein protocol for nuclei isolation from fresh and frozen tissues using OptiPrep® continuous gradient | 1 | dx.doi.org/10.17504/protocols.io.bs99nh96 | https://www.protocols.io/view/daughter-of-frankenstein-protocol-for-nuclei-isola-bs99nh96 | Luciano Martelotto | TITLE: Daughter of Frankenstein protocol for nuclei isolation from fresh and frozen tissues using OptiPrep® continuous gradient
AUTHORS: Luciano Martelotto
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is the result of the combination of various nuclei isolation protocols for single ... | ["[Tissue Homogenization]\nMince/chop tissue with a razor blade to small pieces. The tissue may be as small as a grain of rice.\nFor mincing the tissue, you may take the tube out of ice, however, be quick and return it to ice.", "[Tissue Homogenization]\nAdd of chilled Nuclei EZ Lysis Buffer (supplemented with RNAse I... |
47,042 | Di-electric barrier discharge cold atmospheric plasma device protocol | 1 | dx.doi.org/10.17504/protocols.io.br7am9ie | https://www.protocols.io/view/di-electric-barrier-discharge-cold-atmospheric-pla-br7am9ie | Aoibheann Flynn, Eline Manaloto, Zhonglei He, Patrick J Cullen, James Curtin, Sean Behan | TITLE: Di-electric barrier discharge cold atmospheric plasma device protocol
AUTHORS: Aoibheann Flynn, Eline Manaloto, Zhonglei He, Patrick J Cullen, James Curtin, Sean Behan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Plasma, the fourth state of matter, is an ionized gas that can directly inte... | ["[DBD-CAP device set up]\nPlace the sample plate in a sterile polypropylene case.", "[DBD-CAP device set up]\nPlace a sterile 10mm Perspex layer on top of the polypropylene case.", "[DBD-CAP device set up]\nPlace the negative ground electrode (i.e. the bottom electrode) underneath the polypropylene case.", "[DBD-CAP d... |
null | null | null | dx.doi.org/10.17504/protocols.io.ebdbai6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is a protocol from: <br /><br />Stedman, K. M., K. Porter, and M. L. Dyall-Smith. 2010. Chapter 6: The isolation of viruses infecting Archaea. Manual of Aquatic Viral Ecology. Waco, TX:American Society of Limnology and Oceanography. doi:10.4319/mave.2010.978-0-9845591-0-7<b... | [] |
59,712 | Useful methods: Cefotaxime: a useful antibiotic for duckweed culture management | 1 | null | https://www.protocols.io/view/useful-methods-cefotaxime-a-useful-antibiotic-for-b6i8rchw | Eric Lam, Kenneth Acosta | TITLE: Useful methods: Cefotaxime: a useful antibiotic for duckweed culture management
AUTHORS: Eric Lam, Kenneth Acosta
[DESCRIPTION]
This protocol describes about cefotaxime a useful antibiotic for duckweed culture management. It contains protocols from the The International Steering Committee on Duckweed Research a... | ["[Preparation of Cefotaxime Stock Solution] Add 1 g of cefotaxime (GoldBio; Catalog # C-104) to 10 mL sterile H2O. Dissolve completely.", "[Preparation of Cefotaxime Stock Solution] Filter sterilize solution using 0.22 μm syringe filter.", "[Preparation of Cefotaxime Stock Solution] Aliquot into 1-mL centrifuge tubes.... |
37,995 | test_protocol1 | 1 | null | https://www.protocols.io/view/test-protocol1-bhcjj2un | Abby Moore | TITLE: test_protocol1
AUTHORS: Abby Moore
[DESCRIPTION]
Descript for sub-protocol
[STEPS]
1. step 1 | ["step 1"] |
null | null | null | dx.doi.org/10.17504/protocols.io.mmyc47w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><u>Background</u>: Common ragweed, <em>Ambrosia artemisiifolia </em>L., is an economically important worldwide plant invader, due to its huge production of seeds and allergenic pollen, while its range and impact are expected to increase in future. Knowledge of the population ... | [] |
39,405 | Zielh neelsen for coccidia Agudelo y Montoya, 2004 | 1 | dx.doi.org/10.17504/protocols.io.biqmkdu6 | https://www.protocols.io/view/zielh-neelsen-for-coccidia-agudelo-y-montoya-2004-biqmkdu6 | Javier Antonio Benavides | TITLE: Zielh neelsen for coccidia Agudelo y Montoya, 2004
AUTHORS: Javier Antonio Benavides
[STEPS]
?. Place a drop of 0.85% saline on a new, fat-free, dry slide.
?. Thoroughly homogenize the stool sample to be examined with a wooden toothpick
?. Place the wooden toothpick impregnated with the stool sample in the drop... | ["Place a drop of 0.85% saline on a new, fat-free, dry slide.", "Thoroughly homogenize the stool sample to be examined with a wooden toothpick", "Place the wooden toothpick impregnated with the stool sample in the drop of saline solution that was put on the slide and homogenize in such a way that it has a spread of app... |
84,844 | In utero inner ear AAV injection | 4 | dx.doi.org/10.17504/protocols.io.kqdg3x62pg25/v1 | https://www.protocols.io/view/in-utero-inner-ear-aav-injection-cw4kxguw | Carla Maria Barbosa Spinola, jacques.boutet-de-monvel, saaid.safieddine, ghizlene.lahlou, Raphaël Etournay | TITLE: In utero inner ear AAV injection
AUTHORS: Carla Maria Barbosa Spinola, jacques.boutet-de-monvel, saaid.safieddine, ghizlene.lahlou, Raphaël Etournay
[DESCRIPTION]
In vivo gene delivery to tissues using adeno-associated vector (AAVs) has revolutionized the field of gene therapy. Yet, while sensorineural hearing... | ["[Preparation of animals and materials] Pregnant female mice must be acclimated to the local animal facility at least 2 days prior to surgery. One day before surgery, weight the mouse, and observe its general behaviour.", "[Preparation of animals and materials] Decontaminate the materials used for surgery. Either chem... |
51,970 | Functional Magnetic Resonance Imaging Under Swallowing Task in Healthy Human Subjects and Gastroparetic Patients | 1 | dx.doi.org/10.17504/protocols.io.x54v9j4d1g3e/v1 | https://www.protocols.io/view/functional-magnetic-resonance-imaging-under-swallo-bwzapf2e | Kun-Han Lu, Kristine Mosier, John Wo, Terry Powley | TITLE: Functional Magnetic Resonance Imaging Under Swallowing Task in Healthy Human Subjects and Gastroparetic Patients
AUTHORS: Kun-Han Lu, Kristine Mosier, John Wo, Terry Powley
[DESCRIPTION]
This protocol described the steps to acquire and analyze functional magnetic resonance imaging data under a swallowing task i... | ["[Subjects] Eight healthy subjects (6 females; 2 males) and 3 gastroparetic patients (2 females; 1 male) participated in this study under research protocols approved by the Institutional Review Board at Indiana University School of Medicine. All healthy subjects did not have a prior diagnosis of neurological, psychiat... |
null | null | null | dx.doi.org/10.17504/protocols.io.gbhbsj6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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null | null | null | dx.doi.org/10.17504/protocols.io.jauciew | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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83,654 | Aspirin for primary prevention of cardiovascular events in patients with chronic kidney disease: an updated meta-analysis | 1 | dx.doi.org/10.17504/protocols.io.261ged63jv47/v1 | https://www.protocols.click/view/aspirin-for-primary-prevention-of-cardiovascular-e-cvxew7je | Ioannis Bellos, Smaragdi Marinaki, Pagona Lagiou, Vassiliki Benetou | TITLE: Aspirin for primary prevention of cardiovascular events in patients with chronic kidney disease: an updated meta-analysis
AUTHORS: Ioannis Bellos, Smaragdi Marinaki, Pagona Lagiou, Vassiliki Benetou
[DESCRIPTION]
The present meta-analysis aims to evaluate the role of aspirin for the primary prevention of cardio... | ["Objective To determine the efficacy and safety of aspirin given for primary prevention of cardiovascular diseases in patients with chronic kidney disease.", "Eligibility criteria The population of the study will consist of patients with diagnosed chronic kidney disease, without evidence of cardiovascular disease at b... |
20,357 | U Mass - Surgery – tail vein injection | null | dx.doi.org/10.17504/protocols.io.x5dfq26 | null | Jason Kim | TITLE: U Mass - Surgery – tail vein injection
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Intravenous administration via tail vein is used to acutely deliver drug, hormones, and adenoassociated v... | ["Place mice in clean cages and keep warm with a heat lamp for ~5 min with constant monitoring to induce vasodilation.", "Transfer mice to a restrainer with a hole for the tail.", "Apply 70% Ethanol wipes to tail and turn 45º for a visible presentation of tail vein.", "Introduce a 27G syringe into tail vein to administ... |
null | null | null | dx.doi.org/10.17504/protocols.io.gc7bszn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>for 500ml (2x250ml)</p>
[STEPS]
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null | null | null | dx.doi.org/10.17504/protocols.io.cpgvjv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Protocol for blunting ends by 3' overhang removal and fill-in of 3' recessed (5' overhang) ends using DNA Polymerase I, Large (Klenow) Fragment (M0210)
[STEPS]
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69,837 | CTAB-based DNA extraction for citrus | 4 | dx.doi.org/10.17504/protocols.io.dm6gpj8jpgzp/v1 | https://www.protocols.io/view/ctab-based-dna-extraction-for-citrus-cgfmttk6 | Shingo Goto | TITLE: CTAB-based DNA extraction for citrus
AUTHORS: Shingo Goto
[DESCRIPTION]
CTAB-based protocols are used for genomic DNA extraction from many kinds of plant species. However, the protocols can’t necessarily completely remove contamination of polysaccharide and RNA in extracted genomic DNA solution. Especially, ci... | ["[Buffer preparation] 2×CTAB solution: 2% (w/v) CTAB, 100mMTris-HCl pH8.0, 1.4M NaCl, 20mM EDTA pH8.0 [1]\nHigh-salt precipitation solution: 1.2M NaCl , 0.8M Sodium citrate [2]\n10 mg/ml RNase (Nippon gene)\nChloroform\nIsopropanol\n70% Ethanol\nTE buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0\nReference\nAllen GC, Flores... |
38,607 | Do the proportion of protocol registrations about systematic reviews outside PROSPERO in medical and biomedical sciences increase over the years?: a meta-epidemiological study protocol | 1 | dx.doi.org/10.17504/protocols.io.bhxpj7mn | https://www.protocols.io/view/do-the-proportion-of-protocol-registrations-about-bhxpj7mn | Masahiro Banno, Yasushi Tsujimoto, Yuki Kataoka | TITLE: Do the proportion of protocol registrations about systematic reviews outside PROSPERO in medical and biomedical sciences increase over the years?: a meta-epidemiological study protocol
AUTHORS: Masahiro Banno, Yasushi Tsujimoto, Yuki Kataoka
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Obj... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.hvfb63n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Luminex Mulliplex Cytokine/Chemokine 17-plex MAG manufacturer's protocol</p>
[STEPS]
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null | null | null | dx.doi.org/10.17504/protocols.io.m4xc8xn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A description of to how to produce and evaluate three-dimensional computerized forensic craniofacial reconstructions (CCFR) using Horos, Blender, Cloud Compare, MakeHuman and Picassa computer programs.</p>
[BEFORE_START]
<p>You need download the software programs: Horos, Ble... | [] |
51,371 | Human Pancreas Processing for Multiple Applications | 1 | dx.doi.org/10.17504/protocols.io.bwejpbcn | https://www.protocols.io/view/human-pancreas-processing-for-multiple-application-bwejpbcn | Diane Saunders, Rita Bottino, Marcela Brissova, Angela Kruse, Jamie Allen, Carrie Romer, Danielle Gutierrez, Jeff Spraggins, Alvin Powers | TITLE: Human Pancreas Processing for Multiple Applications
AUTHORS: Diane Saunders, Rita Bottino, Marcela Brissova, Angela Kruse, Jamie Allen, Carrie Romer, Danielle Gutierrez, Jeff Spraggins, Alvin Powers
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Procuring and preparing human pancreas for pa... | ["[Preparing Pancreatic Cross-Sections]\nUpon arrival, transfer the pancreas and transport solution (UW or HTK) into a dissection tray placed on ice. Transfer enough of the transport solution to keep the pancreas submerged.", "[Preparing Pancreatic Cross-Sections]\nDissect the pancreas by carefully removing duodenum, m... |
40,854 | Study Design (Part 3 of Phase 3 study of Vaccine Candidate for COVID-19) | 1 | dx.doi.org/10.17504/protocols.io.bj5wkq7e | https://www.protocols.io/view/study-design-part-3-of-phase-3-study-of-vaccine-ca-bj5wkq7e | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | TITLE: Study Design (Part 3 of Phase 3 study of Vaccine Candidate for COVID-19)
AUTHORS: Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is Part 3 of "Phase 3 randomized, double-blinded, placebo-controlled trial to evaluate the safety... | [] |
58,931 | DigiGait | 4 | dx.doi.org/10.17504/protocols.io.b5stq6en | https://www.protocols.io/view/digigait-b5stq6en | Haley Geertsma | TITLE: DigiGait
AUTHORS: Haley Geertsma
[DESCRIPTION]
This protocol is used to test mice in the DigiGait behavioural test.
[STEPS]
1. Habituate mice in the testing room for 60 minutes.
2. Place mice on the DigiGait treadmill and start the treadmill at 18-22cm/s. Record their movement for 3 seconds then place them b... | ["Habituate mice in the testing room for 60 minutes.", "Place mice on the DigiGait treadmill and start the treadmill at 18-22cm/s. Record their movement for 3 seconds then place them back into their home cage.", "Analyze 3-second clips with DigiGait Analysis Software."] |
103,035 | Synapto-iATPSnFR2-miRFP670nano3 analysis | 0 | dx.doi.org/10.17504/protocols.io.n92ld8wk9v5b/v1 | https://www.protocols.io/view/synapto-iatpsnfr2-mirfp670nano3-analysis-dgu33wyn | Alexandros C Kokotos, Tim Ryan | TITLE: Synapto-iATPSnFR2-miRFP670nano3 analysis
AUTHORS: Alexandros C Kokotos, Tim Ryan
[DESCRIPTION]
This protocol describes how to analyze imaging experiments using Synapto-iATPSnFR2-miRFP670nano3 from primary neurons.
[STEPS]
SECTION: Synapto-iATPSnFR2-miRFP670nano3 image analysis
1. Open the image stacks to by an... | ["[Synapto-iATPSnFR2-miRFP670nano3 image analysis] Open the image stacks to by analyzed with ImageJ by drag and drop.", "[Synapto-iATPSnFR2-miRFP670nano3 image analysis] Using the far-red miRFP670nano3 channel and blind to the ATPSnFR2 channel, identify nerve terminals by their appearance as fluorescent puncta.", "[Syn... |
62,367 | Female Reproductive System Deceased Donor and Tissue Eligibility Criteria for Penn TMC | 1 | dx.doi.org/10.17504/protocols.io.4r3l2oe2pv1y/v1 | https://www.protocols.io/view/female-reproductive-system-deceased-donor-and-tiss-b857ry9n | kone | TITLE: Female Reproductive System Deceased Donor and Tissue Eligibility Criteria for Penn TMC
AUTHORS: kone
[DESCRIPTION]
This document outlines the required criteria for deceased donor inclusion of the female reproductive system in the Human BioMolecular Atlas Program (HuBMAP) for the University of Pennsylvania Tis... | ["Gift of Life Donor Program (GLDP) transplant coordinators receive annual training by MPI O’Neill in HuBMAP initiative.", "Trained coordinators will evaluate patient for eligibility using criteria detailed in following sections.", "Transplant coordinator will call MPI O’Neill to review inclusion/exclusion criteria and... |
40,929 | ELISA for quantification of human C9 in serum or plasma. | 6 | dx.doi.org/10.17504/protocols.io.bj79krr6 | https://www.protocols.io/view/elisa-for-quantification-of-human-c9-in-serum-or-bj79krr6 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme | TITLE: ELISA for quantification of human C9 in serum or plasma.
AUTHORS: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. An anti-human C9 coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbonate-bicarbonate buffer.
... | ["An anti-human C9 coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbonate-bicarbonate buffer.", "Add 50 µl of human serum or plasma. Human C9 present in the serum or plasma binds to antibodies adsorbed into the microwells.", "The microplate is blocked with 3% non-fat milk-PBS buf... |
44,749 | PBMC- 01b Isolation of human PBMC from Whole Blood | 1 | dx.doi.org/10.17504/protocols.io.bpxmmpk6 | https://www.protocols.io/view/pbmc-01b-isolation-of-human-pbmc-from-whole-blood-bpxmmpk6 | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | TITLE: PBMC- 01b Isolation of human PBMC from Whole Blood
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Separation and purification of PBMC from FRESH BLOOD: list of publ... | ["Put the needed amount of blood sampl into a conical tube.\n50 mL", "Add an equal volume of PBS 1X and mix well.\n{\"blocks\":[{\"key\":\"6d8lo\",\"text\":\"This recepe is used in the following protocols:\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"9q3hu\"... |
47,273 | LAMP/RT-LAMP Buffer protocol | 4 | dx.doi.org/10.17504/protocols.io.bsehnbb6 | https://www.protocols.io/view/lamp-rt-lamp-buffer-protocol-bsehnbb6 | Tamara Matute, Isaac Núñez, Fernan Federici | TITLE: LAMP/RT-LAMP Buffer protocol
AUTHORS: Tamara Matute, Isaac Núñez, Fernan Federici
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the preparation of </span><span style = "font-weight:bold;">reaction buffer</span><span> for homemade LAMP / RT-LAMP reactions which ... | ["[Stock solutions]\nDTT (dithiothreitol)To prepare 10 ml of 1 M solution, weigh 1.54 g of DTT powder and dissolve it completely in 80 mL of H2O. Make up the volume to 10 mL with H2O (it can be done in a 15 ml conical tube). Filter-sterilize the solution with a 0.22 μm filter, make 100 uL - 1 ml aliquots and store at -... |
null | null | null | dx.doi.org/10.17504/protocols.io.g75bzq6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div title="Page 2">
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<p>Housekeeping proteins (HKPs) are routinely used for Western blot normalization. For common HKPs (such as actin, tubulin, or GAPDH), stable protein expression is generally assumed. However, expression of several HKPs is now... | [] |
64,453 | Condor CBD Gummies Amazon Shark TankReviews Benefits,Ingredients,side effects and Is it legitor Does it Really Work [HOAX OR SCAM]{Update 2022}-, What To Know Before Using It??Shark TankReviews Benefits,Ingredients,side effects and Is it legitor Does it Re | 1 | dx.doi.org/10.17504/protocols.io.8epv59o4dg1b/v1 | https://www.protocols.io/view/condor-cbd-gummies-amazon-shark-tankreviews-benefi-ca7dshi6 | Condor CBD Gummies | TITLE: Condor CBD Gummies Amazon Shark TankReviews Benefits,Ingredients,side effects and Is it legitor Does it Really Work [HOAX OR SCAM]{Update 2022}-, What To Know Before Using It??Shark TankReviews Benefits,Ingredients,side effects and Is it legitor Does it Re
AUTHORS: Condor CBD Gummies
[DESCRIPTION]
Condor CBD G... | ["Soothes Your Stress And Anxiety And Tension Quick", "Fantastic For Easing Discomfort/ Body Pains", "Helps In Reducing Back And Neck Pain Rapid", "Lowers Tightness And Swelling", "Excellent For Assisting You Move A Lot More", "Calms Any Kind Of Concerns Or Anxieties You Have", "Aids You Fall And Also Keep Asleep Promp... |
81,634 | TS Spurrs - primary fixation Karnovsky's - tissue (TM - 013) | 4 | dx.doi.org/10.17504/protocols.io.6qpvr4jypgmk/v1 | https://www.protocols.io/view/ts-spurrs-primary-fixation-karnovsky-39-s-tissue-t-ctyawpse | sandra.crameri | TITLE: TS Spurrs - primary fixation Karnovsky's - tissue (TM - 013)
AUTHORS: sandra.crameri
[DESCRIPTION]
This method is used for conventional processing of tissue to Spurr's resin.
[GUIDELINES]
All time are minimum times, it is acceptable to go over time specified for any given step. Good place steps to leave ov... | ["[HEADER] SAN:\n\n\nSPEC No:\n\n\nOPERATOR & STEPS:\n\n\nOPERATOR & STEPS:", "[CONVENTIONAL] 2.5 % volume plus 4 % volume in 0.1 Molarity (m) for at least 40 min", "[CONVENTIONAL] Wash 0.1 Molarity (M) Sorenson's Phosphate Buffer pH 07.2 (300mosmol/kg) for 15 min", "[CONVENTIONAL] 1 % volume in buffer for 60 mi... |
1,545 | protein extraction from cell pallets | 1 | dx.doi.org/10.17504/protocols.io.drh535 | https://www.protocols.io/view/protein-extraction-from-cell-pallets-drh535 | Jacob Waldbauer, Lichun Zhang | TITLE: protein extraction from cell pallets
AUTHORS: Jacob Waldbauer, Lichun Zhang
[DESCRIPTION]
Use LDS buffer to extract protein from cell palllets
[STEPS]
1. Make 1X LDS buffer. In 40mL of 1X buffer, add Tris HCl 0.666g, Tris base 0.682g, LDS 0.8g, EDTA 0.006g, Glycerol 4g.
2. make 1M DTT stock solution in LC/MS... | ["Make 1X LDS buffer. In 40mL of 1X buffer, add Tris HCl 0.666g, Tris base 0.682g, LDS 0.8g, EDTA 0.006g, Glycerol 4g.", "make 1M DTT stock solution in LC/MS water.", "Add 200~500uL of 10mM DTT in 1X LDS buffer to the cell pallet tube.", "Heat sample up at 95ºC for 20 minutes.", "Cool down the sample to room temrature.... |
22,878 | Protocol for bacterial cultivation of nasal and throat samples in the Fit Futures study | null | dx.doi.org/10.17504/protocols.io.2j6gcre | null | Dina Stensen, Lars Småbrekke, Anne-Sofie Furberg, Karina Olsen, Guri Grimnes, Cristopher Sivert Nielsen, Gunnar Skov Simonsen, Johanna UE Sollid | TITLE: Protocol for bacterial cultivation of nasal and throat samples in the Fit Futures study
AUTHORS: Dina Stensen, Lars Småbrekke, Anne-Sofie Furberg, Karina Olsen, Guri Grimnes, Cristopher Sivert Nielsen, Gunnar Skov Simonsen, Johanna UE Sollid
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><sp... | ["[Day 1]\nExamine testnumber and sort the samples. Label the enrichment broth with identical testnumbers. Separate the nasal samples from the throat samples. Nasal swab 1 and throat swab 1 are used. Nasal swab 2 and throat swab 2 are frozen for microbiome analysis.", "[Day 1]\nPlace the samples in a fume hood, open th... |
46,215 | Assessment of Human Islet Composition and Acinar Cell Component by Immunofluorescence Staining | 4 | dx.doi.org/10.17504/protocols.io.brdfm23n | https://www.protocols.io/view/assessment-of-human-islet-composition-and-acinar-c-brdfm23n | IIDP-HIPP | TITLE: Assessment of Human Islet Composition and Acinar Cell Component by Immunofluorescence Staining
AUTHORS: IIDP-HIPP
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;font-style:italic;">This Standard Operating Procedure (SOP) is based on the Vanderbilt Human Islet... | ["[Preparation of Reagents]\nPreparation of Reagents for Immunofluorescence Staining of Islet Cryosections", "[Preparation of Reagents]\n10% Triton X-100 stock () – combine Triton-X-100 and 1X PBS. Mix on shaker for 30 min or until Triton X-100 is completely dissolved and store at for up to 1 month.\n30 mL\n3 mL\n27 ... |
41,169 | mariPOC User manual.5.5.20 | 3 | null | https://www.protocols.io/view/maripoc-user-manual-5-5-20-bkfrktm6 | vincent.degennaro | TITLE: mariPOC User manual.5.5.20
AUTHORS: vincent.degennaro
[STEPS] | [] |
68,493 | Scaffold Mapping Protocol - Version 1.1.1 | 1 | dx.doi.org/10.17504/protocols.io.n2bvj6o35lk5/v3 | https://www.protocols.io/view/scaffold-mapping-protocol-version-1-1-1-ce5mtg46 | Mabelle Lin, Hugh Sorby, Sonia Sharma, David Nickerson | TITLE: Scaffold Mapping Protocol - Version 1.1.1
AUTHORS: Mabelle Lin, Hugh Sorby, Sonia Sharma, David Nickerson
[DESCRIPTION]
The scope of this protocol covers the steps involved in the mapping of image data to organ scaffolds.
[STEPS]
1. Check for the availability of segmentation files for image data.
This scaff... | ["Check for the availability of segmentation files for image data.\n\nThis scaffold mapping protocol requires image data segmentations to be available in the MBF Bioscience Neuromorphological File Format (MBF XML, https://doi.org/10.1007/s12021-021-09530-x). This can be created directly using the MBF segmentation tools... |
64,676 | Western Blot for IP-TDMS Development | 1 | null | https://www.protocols.io/view/western-blot-for-ip-tdms-development-cbecsjaw | Lauren Adams | TITLE: Western Blot for IP-TDMS Development
AUTHORS: Lauren Adams
[DESCRIPTION]
This protocol can be used to determine relative quantities of protein target in IP fractions. Primary antibodies should be selected for the specific protein target.
[STEPS]
1. Take aliquots from IP fractions and add sample loading buffe... | ["Take aliquots from IP fractions and add sample loading buffer to a final concentration of 1X. Boil samples in hot plate at 95ºC for 5-10 min. Briefly, centrifuge samples to ensure sample entire sample is towards bottom of the tube.", "Open gel and place into gel box. Add 1 x MES running buffer, ensuring that there ar... |
null | null | null | dx.doi.org/10.17504/protocols.io.p4hdqt6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
57,200 | Modified NEBNext® VarSkip Short SARS-CoV-2 Library Prep Kit for Illumina Platforms - adapted for wastewater samples | 1 | dx.doi.org/10.17504/protocols.io.b34qqqvw | https://www.protocols.io/view/modified-nebnext-varskip-short-sars-cov-2-library-b34qqqvw | Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim | TITLE: Modified NEBNext® VarSkip Short SARS-CoV-2 Library Prep Kit for Illumina Platforms - adapted for wastewater samples
AUTHORS: Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim
[DESCRIPTION]
PURPOSE:
This method was developed at the FDA’s Center for Food Safety and Applied Nu... | ["[cDNA Synthesis] Gently mix and spin down the LunaScript RT SuperMix reagent. Prepare the cDNA synthesis reaction as described below:\n \n\nFor no template controls, mix the following components:", "[cDNA Synthesis] Incubate reactions in a thermocycler* with the following steps:", "[Targeted cDNA Amplification]", "[T... |
null | null | null | dx.doi.org/10.17504/protocols.io.eyzbfx6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes how to isolate bacteria in order to label its biomass using <sup>13</sup>C-glucose and <sup>15</sup>N-ammonia. The first steps aim to isolate candidate bacteria that are able of growing on the chosen substrates for isotopic labeling.</p>
<p> </p>
<p>Th... | [] |
28,849 | RPA DNA Amplification using TwistAmpTM Basic | null | dx.doi.org/10.17504/protocols.io.8erhtd6 | null | Luc Gabel, Théo Nass | TITLE: RPA DNA Amplification using TwistAmpTM Basic
AUTHORS: Luc Gabel, Théo Nass
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the procedure to perform an RPA DNA Amplification using the TwistAmp</span><span style = "vertical-align:super;">TM</span><span> Basic</span... | ["Prepare the RPA pellet rehydration solution in a 1.5 ml tube following the table in the Materials section.If possible do a Master Mix that will then be divided.", "For each sample, transfer the entire volume (47.5 µl) to a reaction pellet. Pipette up and down until the pellet is fully resuspended.", "Add 2.5 µl of 28... |
68,006 | IB BIO SL IA M23 - A study on the conditions that affect yeast fermentation | 4 | dx.doi.org/10.17504/protocols.io.261geny6jg47/v1 | https://www.protocols.io/view/ib-bio-sl-ia-m23-a-study-on-the-conditions-that-af-cenetdbe | Marcy Roca Cugat | TITLE: IB BIO SL IA M23 - A study on the conditions that affect yeast fermentation
AUTHORS: Marcy Roca Cugat
[DESCRIPTION]
This protocol is designed to aid me in the research for my International Baccalaureate Biology Internal Assignment
[GUIDELINES]
None
[STEPS]
SECTION: Preparation
1. Check that all reagents and... | ["[Preparation] Check that all reagents and materials are ready.", "[Preparation] Assemble the instrumentation", "[Preparation] One end of the flask must be plugged with a holed stopper and a thermometer.\nThe other end should be plugged with a holed stopper and a glass tube.\nThe middle opening should be covered with ... |
35,598 | Artificial Cerebrospinal Fluid VII (ACSF.VII) | null | dx.doi.org/10.17504/protocols.io.beznjf5e | null | Allen Institute for Brain Science | TITLE: Artificial Cerebrospinal Fluid VII (ACSF.VII)
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to prepare Artificial Cerebrospinal Fluid VII (ACSF.VII). ACSF.VII is used for applications including fresh human brain tissue transpo... | [] |
69,740 | Phylogenetic Analysis of Complete Bovine Coronavirus Genome Sequences | 5 | dx.doi.org/10.17504/protocols.io.kqdg3pyeql25/v2 | https://www.protocols.io/view/phylogenetic-analysis-of-complete-bovine-coronavir-cgcktsuw | Aspen M Workman, Tara G. McDaneld, Gregory P P Harhay, John Dustin Loy, Benjamin M. Hause, Subha das | TITLE: Phylogenetic Analysis of Complete Bovine Coronavirus Genome Sequences
AUTHORS: Aspen M Workman, Tara G. McDaneld, Gregory P P Harhay, John Dustin Loy, Benjamin M. Hause, Subha das
[DESCRIPTION]
Bovine coronavirus (BCoV) has spilled over to many species, including humans, where the host range variant coronavi... | ["[Software]", "[Software]", "[MAFFT Alignment] Align using MAFFT v7.450\n\nThe sequences were aligned using the MAFFT accuracy methods ---globalpair as defined in the mafft manual page below \n\n \n\n\nKeep in mind ...\n\nThe commands below generate files that contain both STDOUT (mafft output to the \"terminal\"... |
86,442 | Efficient recovery of complete gut viral genomes by combined short- and long-read sequencing | 5 | dx.doi.org/10.17504/protocols.io.36wgq339klk5/v1 | https://www.protocols.io/view/efficient-recovery-of-complete-gut-viral-genomes-b-cynixvce | chuqingsun | TITLE: Efficient recovery of complete gut viral genomes by combined short- and long-read sequencing
AUTHORS: chuqingsun
[DESCRIPTION]
Current metagenome-assembled human gut phage catalogs contained mostly fragmented genomes. Here, we developed a vigorous gut virome detection procedure involving viral-like particle enr... | ["[Assembly] NGS Assembly", "[Assembly] # TGS assembly", "[Assembly] Hybrid Assembly", "[Additional analysis] Viral annotation", "[Additional analysis] UHGG filteration", "[Additional analysis] checkv", "[Additional analysis] RPKM calculation"] |
82,970 | Hairy root generation in common bean (Phaseolus vulgaris L.) and selection of Agrobacterium rhizogenes clones | 4 | dx.doi.org/10.17504/protocols.io.261ge3bpjl47/v2 | https://www.protocols.click/view/hairy-root-generation-in-common-bean-phaseolus-vul-cu92wz8e | Ronal Pacheco, Georgina Estrada-Navarrete, Noreide Nava, Jorge Solis-Miranda, Carmen Quinto | TITLE: Hairy root generation in common bean (Phaseolus vulgaris L.) and selection of Agrobacterium rhizogenes clones
AUTHORS: Ronal Pacheco, Georgina Estrada-Navarrete, Noreide Nava, Jorge Solis-Miranda, Carmen Quinto
[DESCRIPTION]
The common bean (Phaseolus vulgaris L.) is one of the legumes used to study the molecul... | ["[Seeds disinfection (when necessary)] Immerse the common bean seeds in 96 % volume ethanol for 5 min and wash them three times with sterile water.", "[Seeds disinfection (when necessary)] Immerse the seeds in 2 % volume sodium hypochlorite for 5 min and wash them three times with sterile water.", "[Seeds disinfecti... |
26,685 | HumidOSH: A Self-Contained Environmental Chamber with Controls for Relative Humidity and Fan Speed | null | dx.doi.org/10.17504/protocols.io.6a5hag6 | null | Soon Kiat Lau, Jeyamkondan Subbiah | TITLE: HumidOSH: A Self-Contained Environmental Chamber with Controls for Relative Humidity and Fan Speed
AUTHORS: Soon Kiat Lau, Jeyamkondan Subbiah
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Relative humidity is a measure of water availability in air that can affect physical, biological... | ["[Printed Circuit Boards]\nThere are two printed circuit boards (PCB) used in HumidOSH: the control box PCB and the relative humidity sensor PCB. The PCB design files (https://osf.io/579fq/) can be sent to a PCB manufacturer (e.g. JLCPCB or OSHPark) for manufacturing. When ordering the PCB, it is recommended to order ... |
52,409 | mIHC staining (OHSU, Coussens' lab) SOP (TMA_TNP) | 1 | dx.doi.org/10.17504/protocols.io.bxezpjf6 | https://www.protocols.io/view/mihc-staining-ohsu-coussens-39-lab-sop-tma-tnp-bxezpjf6 | Courtney Betts, Lisa Coussens, Shamilene Sivagnanam, Konjit Betre | TITLE: mIHC staining (OHSU, Coussens' lab) SOP (TMA_TNP)
AUTHORS: Courtney Betts, Lisa Coussens, Shamilene Sivagnanam, Konjit Betre
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes multiplex immunohistochemical staining of human FFPE tissues. A staining "cycle" is defined... | ["[Bake and Deparaffinize slides ]\nIncubate slides at 58-60 for 30 min. Cool for 5 minutes, then bake for another 30 mins.", "[Bake and Deparaffinize slides ]\nDeparaffinize slides:Xylene 2 x 5 min, 100% ETOH 2 x 2 min, 95% ETOH 2 x 2 min, 70% ETOH 2 x 2 min, 50% ETOH 1 x 2 min, diH20 2 x 2 min", "[Bake and Deparaffin... |
26,055 | Humanized Antibody Preparation Strategy | null | dx.doi.org/10.17504/protocols.io.5pfg5jn | null | susan wind | TITLE: Humanized Antibody Preparation Strategy
AUTHORS: susan wind
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">The humanized antibody mainly refers to the antibody which is re-expressed by the mouse monoclonal antibody by gene cloning and DNA reco... | [] |
55,586 | Collection of GBS samples at 35-37 weeks of gestation and isolation | 4 | dx.doi.org/10.17504/protocols.io.b2iaqcae | https://www.protocols.io/view/collection-of-gbs-samples-at-35-37-weeks-of-gestat-b2iaqcae | lydiariver | TITLE: Collection of GBS samples at 35-37 weeks of gestation and isolation
AUTHORS: lydiariver
[DESCRIPTION]
Recto- vaginal samples collections usually practice to pregnant women because Streptococcus agalactiae a bacterial pathogen frequently carried in the normal fecal and/or vaginal flora but also is one of the ... | ["Remove swab from packaging and insert swab 2 cm into vagina, followed by the rectum, insert swab 1 cm through the anal sphincter using the same swab.", "Place the swab (s) into a nonnutritive transport medium. Apropriate transport systems (e.g, Stuarts or Amies with or without charcoal) are commercialy available.", "... |
3,827 | DNase treatment with Invitrogen kit | 1 | dx.doi.org/10.17504/protocols.io.14egn3yml5dy/v1 | https://www.protocols.io/view/dnase-treatment-with-invitrogen-kit-fytbpwn | Anna JSzékely | TITLE: DNase treatment with Invitrogen kit
AUTHORS: Anna JSzékely
[DESCRIPTION]
We use this protocol to remove DNA from DNA/RNA mix isolated with Easy-DNA Kit (see Simultaneous DNA/RNA extraction for filtered water samples protocol).
[BEFORE_START]
Clean all surfaces (bench, pipettes, racks) before starting to ensu... | ["[Mix preparation] Prepare incubation mix (for few samples) and continue at step 5:\n \n \n \nThere are no exact indications for the amount of sample to use but I usually start with the maximum amount (8 µl) and reduce it only for the samples were the test PCR is positive.\n \nFor more samples prepare master mix (MM) ... |
31,539 | Deep Cleaning Bacteria Incubator | null | dx.doi.org/10.17504/protocols.io.ba2tigen | null | Kenneth Schackart | TITLE: Deep Cleaning Bacteria Incubator
AUTHORS: Kenneth Schackart
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details how to deep-clean the bacteria incubator (Fisher Scientific Isotemp 637F). This cleaning should be performed at least every 4 months during regular usage, more fre... | ["[Disassemble]\nBefore beginning any work on the incubator, turn off the power using the switch at the bottom.", "[Disassemble]\nRemove incubator door:Loosen the screws on the inside of the hinges (hold the door so it doesn't fall when released).Slide door out of the hinges by pulling outward.The hinge on the top will... |
48,734 | CD45 Depletion Protocol | 4 | null | https://www.protocols.io/view/cd45-depletion-protocol-btt6nnre | Morrisey Lab | TITLE: CD45 Depletion Protocol
AUTHORS: Morrisey Lab
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">CD45 Depletion Protocol</div></div>
[STEPS] | [] |
25,734 | LIPID-MEDIATED TRANSFECTION OF iPSCs (Basic Protocol 2) | null | dx.doi.org/10.17504/protocols.io.5deg23e | null | Michael S. Fernandopulle, Ryan Prestil, Christopher Grunseich, Chao Wang, Li Gan, Michael E. Ward | TITLE: LIPID-MEDIATED TRANSFECTION OF iPSCs (Basic Protocol 2)
AUTHORS: Michael S. Fernandopulle, Ryan Prestil, Christopher Grunseich, Chao Wang, Li Gan, Michael E. Ward
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the lipid-mediated transfection of iPSCs maintained ... | ["Grow a sufficient number of iPSCs for transfection and prepare cells as for an Accutase split (see Basic Protocol 1).\nOne or two wells of a 6-well dish at 80 % confluency should provide more than enough cells for one transfection.", "Count the cells (Phelan & May, 2015), transfer 1.5 × 106 cells to a 15-ml conical t... |
49,798 | Step 1: Swab sample collection | 1 | null | https://www.protocols.io/view/step-1-swab-sample-collection-buvenw3e | Peter H L Krijger, Tim A Hoek, Sanne Boersma, Lieke I P M Donders, Maaike M C Broeders, Mark Pieterse, Pim W Toonen, Ive Logister, Bram M P Verhagen, Marjon J A M Verstegen, Thomas W van Ravesteyn, Rene J T M Roymans, Francesca Mattiroli, Jo Vandesompele, Monique Nijhuis, Stefan Meijer, Anton van Weert, Edwin Dekker, F... | TITLE: Step 1: Swab sample collection
AUTHORS: Peter H L Krijger, Tim A Hoek, Sanne Boersma, Lieke I P M Donders, Maaike M C Broeders, Mark Pieterse, Pim W Toonen, Ive Logister, Bram M P Verhagen, Marjon J A M Verstegen, Thomas W van Ravesteyn, Rene J T M Roymans, Francesca Mattiroli, Jo Vandesompele, Monique Nijhuis, ... | ["[Sample collection]\nPerson 1 scans the 2D barcode at the bottom of a 0.8 ml Micronic tube prefilled with 0.4 ml virus transport buffer and places it in a Micronic tube rack (tube rack A).", "[Sample collection]\nPerson 1 removes cap from the Micronic tube using the manual recapping device (cap stays on the recapping... |
83,422 | DNA extraction from Salton Sea | 4 | null | https://www.protocols.io/view/dna-extraction-from-salton-sea-cvp6w5re | Ryan Sinclair | TITLE: DNA extraction from Salton Sea
AUTHORS: Ryan Sinclair
[DESCRIPTION]
To extract DNA from environmental microorganisms, we used GenEluteTM Bacterial Genomic DNA KIT (Sigma- Aldrich, Missouri, USA). For field samples, we used the modified gram-positive extraction procedure described by the manufacture. After filtr... | ["[Option if using enrichment] Steps\nPrepare sterile Trypticase Soy Broth (TSB) according to instructions.\nAdd 30 mLof sterile TSB in 50ml centrifuge tube.\nShake sample on vortex for 30 seconds and then add1 mL of sample to the TSB solution.\nLoosen caps and insert into shaker incubator at 37 °C for overnight at 50... |
103,171 | Rotarod test | 0 | dx.doi.org/10.17504/protocols.io.eq2lywe3rvx9/v1 | https://www.protocols.io/view/rotarod-test-dgzb3x2n | Chuyu Chen | TITLE: Rotarod test
AUTHORS: Chuyu Chen
[DESCRIPTION]
Motor learning was assessed with an accelerating rotarod.
[STEPS]
1. The task was done using a rotarod apparatus (Panlab) equipped with a mouse rod (3 cm diameter) and set to 4–40 rpm acceleration over 300 seconds.
2. The task consisted of daily sessions (five t... | ["The task was done using a rotarod apparatus (Panlab) equipped with a mouse rod (3 cm diameter) and set to 4–40 rpm acceleration over 300 seconds.", "The task consisted of daily sessions (five trials per session; intertrial-interval = 15 s, max trial duration = 300 s)", "Mice were placed on the accelerating rotarod. T... |
99,146 | Wake Forest University Health Sciences Manual of Procedures Biospecimen Collection and Processing V5 | 1 | dx.doi.org/10.17504/protocols.io.x54v92yjml3e/v1 | https://www.protocols.io/view/wake-forest-university-health-sciences-manual-of-p-dc3i2yke | Kim Kennedy, Heather Gregory | TITLE: Wake Forest University Health Sciences Manual of Procedures Biospecimen Collection and Processing V5
AUTHORS: Kim Kennedy, Heather Gregory
[DESCRIPTION]
This includes the methods associated with obtaining muscle, blood, and urine along with demographic, clinical, health, and functional data from younger and old... | [] |
36,627 | SPARC Cat - Sham Control Chronic Cat 1 Day 30 | 1 | dx.doi.org/10.17504/protocols.io.bfztjp6n | https://www.protocols.io/view/sparc-cat-sham-control-chronic-cat-1-day-30-bfztjp6n | Brett Hanzlicek, Anna Rietsch, Margot Damaser | TITLE: SPARC Cat - Sham Control Chronic Cat 1 Day 30
AUTHORS: Brett Hanzlicek, Anna Rietsch, Margot Damaser
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a procedure for a sham control chronic cat experiment (Day 30-terminal) for cystotomy (bladder surgery). The cystotomy is per... | ["[Transport Cat]\nTransport cat from housing site to surgery site.", "[Animal Prep and catheter placement]\nAnimal is anesthetized and abdomen is shaved by the vet team. The cat is then moved into the surgery room and attached to monitors by the vet team.", "[Animal Prep and catheter placement]\nDrape animal and perf... |
104,913 | URMC TriState SenNet TMC Mouse Donor Selection Criteria | 0 | dx.doi.org/10.17504/protocols.io.dm6gpz2wdlzp/v1 | https://www.protocols.io/view/urmc-tristate-sennet-tmc-mouse-donor-selection-cri-dipr4dm6 | Gagandeep Kaur, Irfan Rahman | TITLE: URMC TriState SenNet TMC Mouse Donor Selection Criteria
AUTHORS: Gagandeep Kaur, Irfan Rahman
[DESCRIPTION]
This document outlines the required criteria for donor inclusion of mouse lung specimens in the TriState SenNet TMC Biospecimen Core at the University of Rochester, as part of the Cellular Senescence Netw... | ["[Inclusion Criteria] Age: Adult mice. Two age ranges: \n Young Adults : 2-6 months\n Old adults (geriatric) : 18-24 months", "[Inclusion Criteria] Sex: Both sexes were included.", "[Inclusion Criteria] Strai... |
79,501 | Pavlovian Conditioned Approach | 1 | dx.doi.org/10.17504/protocols.io.x54v9yjx4g3e/v2 | https://www.protocols.io/view/pavlovian-conditioned-approach-crvmv646 | lukehann, cpking, Paul Meyer | TITLE: Pavlovian Conditioned Approach
AUTHORS: lukehann, cpking, Paul Meyer
[DESCRIPTION]
Through their association with rewards, reward associated stimuli “cues” can acquire incentive motivational properties (aka incentive value), and thus the ability to attract, reinforce, and motivate behavior (Robinson and Berridg... | ["[Reward Habituation] Give each subject ~25 banana flavored pellets/day in their home cage for the 2 days preceding the start of the Pre-training.", "[Pre-Training] Pre-training consists of a single, ~17.5 minute session (time varies between banana pellets on a VI-30s schedule).", "[Pre-Training] Prior to testing, dow... |
97,609 | SOP – 2-step protein fractionation (Triton) from fly heads | 0 | dx.doi.org/10.17504/protocols.io.81wgbz151gpk/v1 | https://www.protocols.io/view/sop-2-step-protein-fractionation-triton-from-fly-h-dbjh2kj6 | ALFONSO.M.PENA | TITLE: SOP – 2-step protein fractionation (Triton) from fly heads
AUTHORS: ALFONSO.M.PENA
[DESCRIPTION]
SOP – 2-step protein fractionation (Triton) from fly heads
[STEPS]
SECTION: Protocol
4. Collect 20-40 fly heads via Snap Freeze method into a Biomasher tube and transfer tubes to CTRND on dry ice.
SECTION: Protocol... | ["[Protocol] Collect 20-40 fly heads via Snap Freeze method into a Biomasher tube and transfer tubes to CTRND on dry ice.", "[Protocol] Turn on Sorvall ultracentrifuge and set temperature to 4degC, place rotor inside and turn on vacuum to allow internal temperature to set.", "[Protocol] Add 50uL (2.5uL/fly head) of 1% ... |
null | null | null | dx.doi.org/10.17504/protocols.io.un3evgn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | ["{\"blocks\":[{\"key\":\"cqf0c\",\"text\":\" \",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[{\"offset\":0,\"length\":1,\"key\":0}],\"data\":[]},{\"key\":\"e1mab\",\"text\":\"\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"527ob\... |
46,322 | XPRIZE SANATA Protocol for Saliva LFIA Test | 4 | dx.doi.org/10.17504/protocols.io.brgsm3we | https://www.protocols.io/view/xprize-sanata-protocol-for-saliva-lfia-test-brgsm3we | Mario Thomas, Jasmine Sollen, Natalia Ivanova, Michelle Feng, Reda Fayek, Heidi Abdilla, Stephanie Lim, Amanda Naaum, Holly Rumery, Mario Escobar | TITLE: XPRIZE SANATA Protocol for Saliva LFIA Test
AUTHORS: Mario Thomas, Jasmine Sollen, Natalia Ivanova, Michelle Feng, Reda Fayek, Heidi Abdilla, Stephanie Lim, Amanda Naaum, Holly Rumery, Mario Escobar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This procedure outlines the protocol for test... | ["[Setting up the test]\nRefrain from consuming food or beverage (including water) for 30 minutes before providing a saliva sample.\nEnsure the smartphone is fully charged and Bluetooth on the smartphone is turned on. The mobile app should be downloaded and ready to run.\nWhen working with human saliva and other human ... |
47,631 | Efficacy of proton pump inhibitor (PPI) therapy in treating sleep disturbances in patients with gastroesopahgeal reflux disease (GERD): a systematic review and meta-analysis | 1 | dx.doi.org/10.17504/protocols.io.bsrpnd5n | https://www.protocols.io/view/efficacy-of-proton-pump-inhibitor-ppi-therapy-in-t-bsrpnd5n | Yeseong Kim, Fahmi Shibli, Takafumi Kubota | TITLE: Efficacy of proton pump inhibitor (PPI) therapy in treating sleep disturbances in patients with gastroesopahgeal reflux disease (GERD): a systematic review and meta-analysis
AUTHORS: Yeseong Kim, Fahmi Shibli, Takafumi Kubota
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The review questio... | [] |
106,519 | DAB Staining for Tyrosine Hydroxylase (TH) on Free-floating Fixed NHP Brain Tissue | 4 | dx.doi.org/10.17504/protocols.io.4r3l2q1dql1y/v1 | https://www.protocols.io/view/dab-staining-for-tyrosine-hydroxylase-th-on-free-f-dj9x4r7n | Andreea Bostan | TITLE: DAB Staining for Tyrosine Hydroxylase (TH) on Free-floating Fixed NHP Brain Tissue
AUTHORS: Andreea Bostan
[DESCRIPTION]
This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining for Tyrosine Hydroxilase (TH) of free-floating fixed brain tissue sections using the avidin/... | ["[Part I (Day 1)] Bring tissue to Room temperaturein phosphate-buffered saline (PBS) on an orbital shaker for 30 minutes. \n30 min.", "[Part I (Day 1)] Prepare Peroxide Solution (3 % H2O2) in dH2O.\nE.g., for 10 mL 3% H2O2 use:\n1000 µL30% H2O2\n9000 µLdH2O", "[Part I (Day 1)] Prepare Blocking Serum Solution: Normal G... |
null | null | null | dx.doi.org/10.17504/protocols.io.mnfc5bn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
104,428 | Phylogenesis of Small Ruminant Lentiviruses: a systematic review protocol | 1 | dx.doi.org/10.17504/protocols.io.n2bvj8zzwgk5/v2 | https://www.protocols.io/view/phylogenesis-of-small-ruminant-lentiviruses-a-syst-dh8k39uw | Silvia Pavone, Paola Gobbi, Massimiliano Orso, Monica Giammarioli | TITLE: Phylogenesis of Small Ruminant Lentiviruses: a systematic review protocol
AUTHORS: Silvia Pavone, Paola Gobbi, Massimiliano Orso, Monica Giammarioli
[DESCRIPTION]
Small ruminant lentiviruses (SRLV) include the closely related Visna-Maedi virus (VMV) and caprine arthritis encephalitis virus (CAEV), which infect ... | ["[INTRODUCTION] Rationale\nSmall ruminant lentiviruses (SRLVs) are members of the Retroviridae family and Lentivirus genus comprising the closely related Visna-Maedi virus (VMV) and caprine arthritis encephalitis virus (CAEV), which infect sheep and goats.1 These viruses cause huge economic losses in the small ruminan... |
95,170 | New trends in the minimally invasive repair of abdominal wall defects | 1 | dx.doi.org/10.17504/protocols.io.eq2lyjk3wlx9/v1 | https://www.protocols.io/view/new-trends-in-the-minimally-invasive-repair-of-abd-c87azzie | Francesco Ferrara | TITLE: New trends in the minimally invasive repair of abdominal wall defects
AUTHORS: Francesco Ferrara
[DESCRIPTION]
In the last years several authors have proposed different names to describe their minimally invasive approaches, other than the classic laparoscopic techniques like IPOM and IPOM plus, for the treatme... | ["[Methods] A literature search was performed by two reviewers in December 2023 including articles from the 01st January 2013 to the 15th December 2023 and using the following databases:\nScopus, MEDLINE/Pubmed, Cochrane Library, and Web of Science. The following Medical Subjects Heading (MeSH) terms were used: ((minim... |
null | null | null | dx.doi.org/10.17504/protocols.io.jdaci2e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Methods and procedures appeared in this study.</p>
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.hu2b6ye | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
81,614 | TS Procure 812 - primary fixation Karnovsky's - tissue (TM - 013) | 4 | dx.doi.org/10.17504/protocols.io.e6nvwj342lmk/v1 | https://www.protocols.io/view/ts-procure-812-primary-fixation-karnovsky-39-s-tis-ctxnwpme | sandra.crameri | TITLE: TS Procure 812 - primary fixation Karnovsky's - tissue (TM - 013)
AUTHORS: sandra.crameri
[DESCRIPTION]
The method is used to process conventionally EM fixed 1mm3 tissues to Procure 812 resin blocks.
[GUIDELINES]
Sample is not optimal for EM, best to dissect and use only the outer 2mm of tissue mass. Samp... | ["[HEADER] SAN:\n\n\n\n\nSPEC No:\n\n\n\n\nOPERATOR & STEPS:\n\n\n\n\nOPERATOR & STEPS:", "[Conventional] Karnovsky's fixative: 2.5 % volume and 4 % volume in 0.1 Molarity (M) Sorenson's Phosphate buffer (pH 7.2, 300mosmol/kg) for at least 40 min or overnight", "[Conventional] 0.1 Molarity (M) buffer 15 min", "[Co... |
38,161 | Generation and Sonication of α-synuclein Fibrils | 1 | dx.doi.org/10.17504/protocols.io.bhhrj356 | https://www.protocols.io/view/generation-and-sonication-of-synuclein-fibrils-bhhrj356 | Vijay Singh, Marta Castellana-Cruz, Nunilo Cremades, Laura Volpicelli-Daley | TITLE: Generation and Sonication of α-synuclein Fibrils
AUTHORS: Vijay Singh, Marta Castellana-Cruz, Nunilo Cremades, Laura Volpicelli-Daley
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Animal models that accurately recapitulate the accumulation of alpha-synuclein (α-syn) inclusions, progressive ... | ["[Making fibrils]\nThaw monomer .\non ice", "[Making fibrils]\nSpin monomer for at max speed on a benchtop centrifuge ( - depending on centrifuge) at .\nCentrifuge: 15000 34\nCentrifuge: 20000 34\n4 °C\nThis step removes large fibrils or aggregates that may have been generated when storing and thawing high concentra... |
21,001 | UC Davis - Lipoprotein Binding Protein (LBP)-Endotoxemia Assay | null | dx.doi.org/10.17504/protocols.io.yrhfv36 | null | Trina Knotts | TITLE: UC Davis - Lipoprotein Binding Protein (LBP)-Endotoxemia Assay
AUTHORS: Trina Knotts
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Plasma samples will be assayed for lipopolysaccharide binding protein (LBP) as... | ["Prepare kit reagents.", "Dilute mouse plasma or serum samples 1:800.", "Add 100 μl of standards (50, 25, 12.5, 6.25, 3.12, 1.56 ng/ml) or diluted samples in duplicate into the corresponding wells of the precoated modules and incubate for one hour at room temperature and shaking (300rpm).", "Wash 3X with Wash Buffer."... |
41,263 | nifH amplification for Illumina sequencing | 4 | dx.doi.org/10.17504/protocols.io.bkipkudn | https://www.protocols.io/view/nifh-amplification-for-illumina-sequencing-bkipkudn | Estelle Bigeard, Adriana Lopes Dos Santos, Catherine Ribeiro | TITLE: nifH amplification for Illumina sequencing
AUTHORS: Estelle Bigeard, Adriana Lopes Dos Santos, Catherine Ribeiro
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">For metabarcoding purpose, the first step involves the amplification by PCR of a given gene region (for example V4 or V9 region of ... | ["[PCR amplification]\nBelow is a suggestion of protocol for nifH amplification from sorted cells and total genomic DNA. This protocol has been tested and used with success.However, before starting the library preparation, few samples from the batch (es) to be analyze should be selected and the PCR conditions should be... |
null | null | null | dx.doi.org/10.17504/protocols.io.psydnfw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes how to make chemically competent <em>Escherichia coli</em> cell.</p>
<p>For the protocol <em></em><em>E. coli</em> strain NEB Tubo was taken.</p>
[BEFORE_START]
<p>Make sure you have the following solutions:</p>
<p> </p>
<ul>
<li><strong>TYM</strong>
... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.k46cyze | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>For promoting the successful aging of elderly residents of Chang Gung Silver Village in Taiwan, five interactive exergames were developed to promote the well-being of the elderly. The exergames included both physical games and cognitive games and were implemented using variou... | [] |
103,806 | CutRun (Histone Modification) Library Preparation | 0 | dx.doi.org/10.17504/protocols.io.261ge523dg47/v1 | https://www.protocols.io/view/cutrun-histone-modification-library-preparation-dhk634ze | Yanming Li | TITLE: CutRun (Histone Modification) Library Preparation
AUTHORS: Yanming Li
[DESCRIPTION]
This is a modified NEB library protocol for CutRun DNA. This protocol has been tested on H3K4Me3, H3K27Ac, H3K27Me3 CutRun. For the transcription factors CutRun, you may check: https://www.protocols.io/view/library-prep-for-cut-... | ["[End Repair (Green)] Add the following components to a sterile nuclease-free tube:", "[End Repair (Green)] Set a 100 µl or 200 µl pipette to 50 µl and then pipette the entire volume up and down at least 10 times to mix thoroughly.\nPerform a quick spin to collect all liquid from the sides of the tube", "[End Repair (... |
null | null | null | dx.doi.org/10.17504/protocols.io.tb2eiqe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The Modified Ziehl-Neelsen stain (mZN stain) is a type of differential bacteriological stain used to identify acid-fast organisms, mainly <em>Mycobacteria</em>. Acid fast organisms are those which are capable of retaining the primary stain when treated with an acid (<em>fast=... | [] |
28,941 | Microfluidic channels wax priming | null | dx.doi.org/10.17504/protocols.io.8hmht46 | null | Jorge Fernández, Manuela de las Casas | TITLE: Microfluidic channels wax priming
AUTHORS: Jorge Fernández, Manuela de las Casas
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Procedure for printing microfluidic hydrophobic channels in nitrocellulose membranes.</div><div class = "text-block">This protocol depicts the steps for easily pri... | ["Set the hot plate to 105ºC or at least warm enough to melt the wax. Cut a small amount from one of the wax pencils and place it on a Petri dish. Set the Petri dish on the hot plate and wait for the wax to melt.", "Cut to their appropiate size the nitrocellulose pieces to prime with the designed microfluidic path.", ... |
55,907 | Sample Protocol | 4 | dx.doi.org/10.17504/protocols.io.b2ubqesn | https://www.protocols.io/view/sample-protocol-b2ubqesn | Josh | TITLE: Sample Protocol
AUTHORS: Josh
[DESCRIPTION]
abstract
[STEPS]
SECTION: This is a section
1. This is a step
SECTION: This is a section
2. Second step
SECTION: Another Section
3. Another step
SECTION: Another Section
4.
| ["[This is a section] This is a step", "[This is a section] Second step", "[Another Section] Another step", "[Another Section]"] |
66,352 | Condor CBD Gummies Website | 1 | dx.doi.org/10.17504/protocols.io.5jyl89z98v2w/v1 | https://www.protocols.io/view/condor-cbd-gummies-website-cc2qsydw | condorcbdbit | TITLE: Condor CBD Gummies Website
AUTHORS: condorcbdbit
[DESCRIPTION]
Condor CBD Gummies Website presents the new recipe with ultra speed to recuperate your wellbeing. Would you like to understand what CBD is like? It very well may be you are confounded and feel somewhat unsure on the off chance that this item can h... | [] |
55,695 | Production of The Aluminum Nitric-acid Keg (TANK) for mitigating explosions during the oxidation of black carbon into benzenepolycarboxylic acid (BPCA) molecular markers | 6 | dx.doi.org/10.17504/protocols.io.eq2lynz4qvx9/v1 | https://www.protocols.io/view/production-of-the-aluminum-nitric-acid-keg-tank-fo-b2mpqc5n | Madelyn Miller, Alex Collins, Riley Barton, Morgan Schaller, Sasha Wagner | TITLE: Production of The Aluminum Nitric-acid Keg (TANK) for mitigating explosions during the oxidation of black carbon into benzenepolycarboxylic acid (BPCA) molecular markers
AUTHORS: Madelyn Miller, Alex Collins, Riley Barton, Morgan Schaller, Sasha Wagner
[DESCRIPTION]
Black carbon is often measured using the benz... | ["[Gather materials and equipment] A more detailed list of the necessary materials and equipment, including recommendations and alternatives, can be found in the \"Materials\" section.\n\nMaterials\nAluminum Blocks \n - 1 for Lid\n - 1 for Body\n2 Alignment post rods\n2 Folding chest handles and corresp... |
40,532 | 616.1 URMC HTC BSL2+ Formalin-Inflated, Paraffin-Embedded Human Lung Tissue | 4 | dx.doi.org/10.17504/protocols.io.bjtuknnw | https://www.protocols.io/view/616-1-urmc-htc-bsl2-formalin-inflated-paraffin-emb-bjtuknnw | Gloria S Pryhuber, Heidie Huyck, Lisa Rogers | TITLE: 616.1 URMC HTC BSL2+ Formalin-Inflated, Paraffin-Embedded Human Lung Tissue
AUTHORS: Gloria S Pryhuber, Heidie Huyck, Lisa Rogers
[DESCRIPTION]
Processing of Formalin Fixed Lung and Non-Lung Tissue for the LungMAP HTC
[GUIDELINES]
Scientific Principles or Validation of Procedures :
CDC. “COVID-19 Pers... | ["[Record Process] Record details of procedure in Worksheet or Directly in Inventory or ELN", "[Record Process] Keep photographic recording of inflation and blocking of lung lobe and tissue", "[Inflation Fixed (Formalin) Lung Lobe] Inflation fixation procedure should be accomplished in a biosafety cabinet with the oper... |
57,795 | Maintenance and inactivation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell culture | 2 | dx.doi.org/10.17504/protocols.io.b4pbqvin | https://www.protocols.io/view/maintenance-and-inactivation-of-mouse-embryonic-fi-b4pbqvin | Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner | TITLE: Maintenance and inactivation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell culture
AUTHORS: Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This collection describes the maintenance and inactivation of mouse embryonic fibroblasts (ME... | [] |
75,821 | SOP - Lysis C plate based DNA extraction | 4 | dx.doi.org/10.17504/protocols.io.ewov1o3molr2/v1 | https://www.protocols.io/view/sop-lysis-c-plate-based-dna-extraction-cnamvac6 | Petra Korlević, Mara Lawniczak | TITLE: SOP - Lysis C plate based DNA extraction
AUTHORS: Petra Korlević, Mara Lawniczak
[DESCRIPTION]
This custom minimally morphologically destructive DNA extraction protocol was developed for malaria transmitting Anopheles mosquitoes as a cheap and fast alternative to kit-based approaches. DNA extracted using this m... | ["[Day 1 (buffer preparation and sample incubation)] Prepare an adequate volume of lysis buffer C in a 15 mL or 50 mL falcon tube using the following table (make sure to adjust the initial concentration of stock components if they differ):\n \n ComponentStock or LOT No.Initial conc.Final conc.Final volume (single ... |
62,996 | Oprah Winfrey Weight Loss : Scam Alert {There Are So Many Duplicate Products} | 1 | dx.doi.org/10.17504/protocols.io.bp2l61md5vqe/v1 | https://www.protocols.io/view/oprah-winfrey-weight-loss-scam-alert-there-are-so-b9rur56w | roxyhimr | TITLE: Oprah Winfrey Weight Loss : Scam Alert {There Are So Many Duplicate Products}
AUTHORS: roxyhimr
[DESCRIPTION]
Check Available Discount Price For Oprah Winfrey Weight Loss
AMAZING BENEFITS OF Oprah Winfrey Weight Loss
Regular intake of keto gummies offers rapid weight loss.
Keto gummies provide flattened bell... | ["[Oprah Winfrey Weight Loss]"] |
78,766 | Prepare Samples for Miseq | 4 | dx.doi.org/10.17504/protocols.io.81wgbyknyvpk/v2 | https://www.protocols.io/view/prepare-samples-for-miseq-cq6nvzde | FishFloorUCL | TITLE: Prepare Samples for Miseq
AUTHORS: FishFloorUCL
[DESCRIPTION]
(MiSeq nano V2 chip 300 cycle), MiSeq multiplexes sequences, allowing you to DNA sequence multiple samples at once. This is particularly useful for genotyping fish with mosaic genotypes, such as F0 knockouts.
@FishFloorUCL
[STEPS]
SECTION: Design ... | ["[Design Miseq Primers] Design primers surrounding your site of interest\n\nOptimal amplicon size 200bp\nAs far as possible, the site of interest should be in the middle of the designed amplicon. (This is so that it has the best chance of being read by both the forward and reverse reads)\nIf possible, try and avoid de... |
41,985 | Rosbash/Janelia StickLAMP Protocol | 4 | dx.doi.org/10.17504/protocols.io.bk89kzz6 | https://www.protocols.io/view/rosbash-janelia-sticklamp-protocol-bk89kzz6 | Albert Yu, Tim Brown, Jasmine Quynh Le, Kristina Galatsis, Michael Rosbash | TITLE: Rosbash/Janelia StickLAMP Protocol
AUTHORS: Albert Yu, Tim Brown, Jasmine Quynh Le, Kristina Galatsis, Michael Rosbash
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A protocol for the detection of SARS-CoV-2 from saliva samples featuring a rapid purification step and a high-contrast colori... | ["[Saliva Collection]\nInstruct patient to avoid food, drink, toothbrushing, and nasal sprays for a minimum of prior to sample collection", "[Saliva Collection]\nBegin pooling saliva in your mouth. Saliva production can be stimulated by thinking about food, or about the saliva collection itself.", "[Saliva Collection... |
48,708 | BPHL SARS-CoV-2 Tiled Amplicon Illumina Sequencing | 4 | dx.doi.org/10.17504/protocols.io.bttcnniw | https://www.protocols.io/view/bphl-sars-cov-2-tiled-amplicon-illumina-sequencing-bttcnniw | Jason Blanton | TITLE: BPHL SARS-CoV-2 Tiled Amplicon Illumina Sequencing
AUTHORS: Jason Blanton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol details the Florida Department of Health's Bureau of Public Health Laboratories' (BPHL) wet lab portion of our SARS-CoV-2 next generation sequencing w... | ["[RNA Extraction]\nExtract RNA from positive COVID-19 clinical specimens with the KingFisher Flex instrument using the Applied Biosystems™MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit and its associated protocol.", "[cDNA Generation]\ncDNA from RNA from any extraction method is produced using with th... |
36,951 | Differentiation of human induced pluripotent stem cells to neural stem cells | 1 | dx.doi.org/10.17504/protocols.io.bgbxjspn | https://www.protocols.io/view/differentiation-of-human-induced-pluripotent-stem-bgbxjspn | Malin Andersson, Alexandra Neaverson, Adam Hunter, Ben Newman, Luke Foulser, Andy Day, Minal Patel, Sebastian Gerety | TITLE: Differentiation of human induced pluripotent stem cells to neural stem cells
AUTHORS: Malin Andersson, Alexandra Neaverson, Adam Hunter, Ben Newman, Luke Foulser, Andy Day, Minal Patel, Sebastian Gerety
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol outlines the steps of differ... | ["[Passaging iPSC for Neural Induction]\nPreparationCoat Falcon 6 well plates with Vitronectin diluted 1:50 in DPBS(-/-) and incubate for at least 2 hours at 37ºC.", "[Neural Induction (Day 0 - Day 9)]\nPreparation: Make up E6 base media. Excess media can be aliquotted into appropriate volume (e.g. 100ml) and stored ... |
null | null | null | dx.doi.org/10.17504/protocols.io.in9cdh6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
28,363 | MojoSort™ Mouse CD11c Nanobeads Protocol | null | dx.doi.org/10.17504/protocols.io.7xjhpkn | null | Sam Li | TITLE: MojoSort™ Mouse CD11c Nanobeads Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Product description and procedure summary:</span><span> The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4 mL in a 5 mL (12 x 75 mm) polypropylene tube.Note: Keep MojoSort™ Buffer on ice throughout the procedure.", "Filter the cells with... |
106,996 | MagMAX Enrichment & Extraction | 0 | dx.doi.org/10.17504/protocols.io.bp2l62ew1gqe/v1 | https://www.protocols.io/view/magmax-enrichment-amp-extraction-dkqu4vww | Victor Mabasa | TITLE: MagMAX Enrichment & Extraction
AUTHORS: Victor Mabasa
[DESCRIPTION]
The Applied Biosystems MagMAX Wastewater Ultra Nucleic Acid Isolation Kit (Cat. No. A52610) is specifically designed for virus enrichment and total nucleic acid extraction from 10 mL wastewater samples. The purified nucleic acids suit vari... | ["[Enrichment process] Set up and label the Sample, Lysis and Tip Comb plates outside of the instrument according to the following table:", "[Enrichment process] Select the appropriate program on the instrument (Wastewater_10mL_Virus_Isolation)", "[Enrichment process] Start the run, then load the prepared sample and pr... |
null | null | null | dx.doi.org/10.17504/protocols.io.utgewjw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Appendix 2.
Overview of 9 Steps of Clinical Reasoning Process including the Variables of Quality Indicators for Physiotherapy Care Process of Patients with Whiplash-Associated Disorders (WAD) for Analysis
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.pt9dnr6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A protocoll outlining the Preperation and transformation of chemo-competent cells for <em>Vibrio natriegens</em></p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
82,914 | Expression and purification for cryo-EM samples of FIP200NTD:ATG13(363-517)-ULK1(MIT) complexes | 1 | dx.doi.org/10.17504/protocols.io.e6nvwjxw7lmk/v1 | https://www.protocols.io/view/expression-and-purification-for-cryo-em-samples-of-cu8awzse | Minghao Chen, Xuefeng Ren | TITLE: Expression and purification for cryo-EM samples of FIP200NTD:ATG13(363-517)-ULK1(MIT) complexes
AUTHORS: Minghao Chen, Xuefeng Ren
[DESCRIPTION]
Expression and purification for cryo-EM samples of FIP200NTD:ATG13(363-517)-ULK1(MIT) complexes
[STEPS]
SECTION: Expression
1. Transfect HEK GNTi cells at concentrati... | ["[Expression] Transfect HEK GNTi cells at concentration of 2 × 10^6 cells/ml", "[Expression] Dilute PEI with Warm Hybridoma-SFM(1X)", "[Expression] In a separate tube, dilute DNA with Hybridoma-SFM(1X)", "[Expression] Add PEI to DNA dilution. Incubate mixture for 30 min at 37 °C", "[Expression] Add mixture to cells. ... |
93,441 | Sample Preparation and 3D-SIM fixed-cell imaging of FTH1 and Filipin in NPC1 and NPC2 mutants | 4 | dx.doi.org/10.17504/protocols.io.3byl4qyjzvo5/v1 | https://www.protocols.io/view/sample-preparation-and-3d-sim-fixed-cell-imaging-o-c7g9zjz6 | Felix Kraus | TITLE: Sample Preparation and 3D-SIM fixed-cell imaging of FTH1 and Filipin in NPC1 and NPC2 mutants
AUTHORS: Felix Kraus
[DESCRIPTION]
Protocol for the evaluation of pUb kinetics using 3D-SIM
[STEPS]
SECTION: Seeding of HeLa cells
1. Wash HeLa TMEM192-3xHA control and NPC1, NPC2 mutants with 1xPBS
SECTION: Seeding o... | ["[Seeding of HeLa cells] Wash HeLa TMEM192-3xHA control and NPC1, NPC2 mutants with 1xPBS", "[Seeding of HeLa cells] Add Trypsin to cells for 5 min and incubate at 37°C to dissociate cells from plastic well", "[Seeding of HeLa cells] Resuspend cells in 1 mL DMEM media", "[Seeding of HeLa cells] Count cells", "[Seeding... |
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