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Genetic engineering is the science of manipulating genetic material of an organism. The first artificial genetic modification accomplished using biotechnology was transgenesis, the process of transferring genes from one organism to another, first accomplished by Herbert Boyer and Stanley Cohen in 1973. It was the result of a series of advancements in techniques that allowed the direct modification of the genome
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Human genetic enhancement or human genetic engineering refers to human enhancement by means of a genetic modification. This could be done in order to cure diseases (gene therapy), prevent the possibility of getting a particular disease (similarly to vaccines), to improve athlete performance in sporting events (gene doping), or to change physical appearance, metabolism, and even improve physical capabilities and mental faculties such as memory and intelligence. These genetic enhancements may or may not be done in such a way that the change is heritable (which has raised concerns within the scientific community)
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Multiplexed Accurate Genome Editing with Short, Trackable, Integrated Cellular barcodes (MAGESTIC) is a platform that builds on the CRISPR/Cas technique. It further improves CRISPR/Cas by making the gene-editing process more precise. It also increases cell survival during the editing process up to sevenfold
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RNA editing (also RNA modification) is a molecular process through which some cells can make discrete changes to specific nucleotide sequences within an RNA molecule after it has been generated by RNA polymerase. It occurs in all living organisms and is one of the most evolutionarily conserved properties of RNAs. RNA editing may include the insertion, deletion, and base substitution of nucleotides within the RNA molecule
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2A self-cleaving peptides, or 2A peptides, is a class of 18–22 aa-long peptides, which can induce ribosomal skipping during translation of a protein in a cell. These peptides share a core sequence motif of DxExNPGP, and are found in a wide range of viral families. 2A peptides are used to help generate polyproteins by causing the ribosome to fail at making a peptide bond
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An adapter or adaptor, or a linker in genetic engineering is a short, chemically synthesized, single-stranded or double-stranded oligonucleotide that can be ligated to the ends of other DNA or RNA molecules. Double stranded adapters can be synthesized to have blunt ends to both terminals or to have sticky end at one end and blunt end at the other. For instance, a double stranded DNA adapter can be used to link the ends of two other DNA molecules (i
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Anti-CRISPR (Anti-Clustered Regularly Interspaced Short Palindromic Repeats or Acr) is a group of proteins found in phages, that inhibit the normal activity of CRISPR-Cas, the immune system of certain bacteria. CRISPR consists of genomic sequences that can be found in prokaryotic organisms, that come from bacteriophages that infected the bacteria beforehand, and are used to defend the cell from further viral attacks. Anti-CRISPR results from an evolutionary process occurred in phages in order to avoid having their genomes destroyed by the prokaryotic cells that they will infect
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Artificially Expanded Genetic Information System (AEGIS) is a synthetic DNA analog experiment that uses some unnatural base pairs from the laboratories of the Foundation for Applied Molecular Evolution in Gainesville, Florida. AEGIS is a NASA-funded project to try to understand how extraterrestrial life may have developed. The system uses twelve different nucleobases in its genetic code
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Assisted reproductive technology (ART) includes medical procedures used primarily to address infertility. This subject involves procedures such as in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), cryopreservation of gametes or embryos, and/or the use of fertility medication. When used to address infertility, ART may also be referred to as fertility treatment
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Biostrophin is a drug which may serve as a vehicle for gene therapy, in the treatment of Duchenne and Becker muscular dystrophy. As mutations in the gene which codes for the protein dystrophin is the underlying defect responsible for both disorders, biostrophin will deliver a genetically-engineered, functional copy of the gene at the molecular level to affected muscle cells. Dosage, as well as a viable means for systemic release of the drug in patients, is currently being investigated with the use of both canine and primate animal models
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Blasticidin S is an antibiotic that is used in biology research for selecting cells in cell culture. Cells of interest can express the blasticidin resistance genes BSD or bsr, and can then survive treatment with the antibiotic. Blasticidin S is a nucleoside analogue antibiotic, resembling the nucleoside cytidine
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The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain, but other organisms such as yeast may also be used. DNA of transformation is ligated into a vector
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Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense of certain bacteria against DNA viruses and plasmids, and is heavily utilized in genetic engineering applications. Its main function is to cut DNA and thereby alter a cell's genome. The CRISPR-Cas9 genome editing technique was a significant contributor to the Nobel Prize in Chemistry in 2020 being awarded to Emmanuelle Charpentier and Jennifer Doudna
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Chemically Induced Dimerization (CID) is a biological mechanism in which two proteins bind only in the presence of a certain small molecule, enzyme or other dimerizing agent. Genetically engineered CID systems are used in biological research to control protein localization, to manipulate signalling pathways and to induce protein activation. History The first small molecule CID system was developed in 1993 and used FK1012, a derivative of the drug tacrolimus (FK506), to induce homo-dimerization of FKBP
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Chromosomal landing is a genetic technique used to identify and isolate clones in a genetic library. Chromosomal landing reduces the problem of analyzing large, and/or highly repetitive genomes by minimizing the need for chromosome walking. It is based on the principle that the expected average between-marker distances can be smaller than the average insert length of a clone library containing the gene of interest
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Cisgenesis is a product designation for a category of genetically engineered plants. A variety of classification schemes have been proposed that order genetically modified organisms based on the nature of introduced genotypical changes, rather than the process of genetic engineering. Cisgenesis (etymology: cis = same side; and genesis = origin) is one term for organisms that have been engineered using a process in which genes are artificially transferred between organisms that could otherwise be conventionally bred
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Conditional gene knockout is a technique used to eliminate a specific gene in a certain tissue, such as the liver. This technique is useful to study the role of individual genes in living organisms. It differs from traditional gene knockout because it targets specific genes at specific times rather than being deleted from beginning of life
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A cosmid is a type of hybrid plasmid that contains a Lambda phage cos sequence. They are often used as a cloning vector in genetic engineering. Cosmids can be used to build genomic libraries
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Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems
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CRISPR () (an acronym for clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. These sequences are derived from DNA fragments of bacteriophages that had previously infected the prokaryote. They are used to detect and destroy DNA from similar bacteriophages during subsequent infections
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CRISPR-Cas design tools are computer software platforms and bioinformatics tools used to facilitate the design of guide RNAs (gRNAs) for use with the CRISPR/Cas gene editing system. CRISPR-Cas The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR associated nucleases) system was originally discovered to be an acquired immune response mechanism used by archaea and bacteria. It has since been adopted for use as a tool in the genetic engineering of higher organisms
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CRISPR gene editing (pronounced "crisper") is a genetic engineering technique in molecular biology by which the genomes of living organisms may be modified. It is based on a simplified version of the bacterial CRISPR-Cas9 antiviral defense system. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added in vivo
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CRISPR-Display (CRISP-Disp) is a modification of the CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) system for genome editing. The CRISPR/Cas9 system uses a short guide RNA (sgRNA) sequence to direct a Streptococcus pyogenes Cas9 nuclease, acting as a programmable DNA binding protein, to cleave DNA at a site of interest. CRISPR-Display, in contrast, uses a nuclease deficient Cas9 (dCas9) and an engineered sgRNA with aptameric accessory RNA domains, ranging from 100bp to 5kb, outside of the normal complementary targeting sequence
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Nutritional yeast (also known as nooch) is a deactivated yeast, often a strain of Saccharomyces cerevisiae, that is sold commercially as a food product. It is sold in the form of yellow flakes, granules, or powder and can be found in the bulk aisle of most natural food stores. It is popular with vegans and vegetarians and may be used as an ingredient in recipes or as a condiment
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Oyster sauce describes a number of sauces made by cooking oysters. The most common in modern use is a viscous dark brown condiment made from oyster extracts, sugar, salt and water thickened with corn starch. Bear in mind that original oyster sauce reduced the unrefined sugar through heating, resulting in a naturally thick sauce due to caramelization, not the addition of corn starch
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Prahok (; Khmer: ប្រហុក, romanized: prâhŏk, IPA: [prɑːhok]) is a salted and fermented fish paste (usually of snakeheads) used in Cambodian cuisine as a seasoning or a condiment. It originated as a way of preserving fish during the months when fresh fish was not available in abundant supply. Because of its saltiness and strong flavor, it was used as an addition to many meals in Cambodian cuisine, such as soups and sauces
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Yeast extracts consist of the cell contents of yeast without the cell walls; they are used as food additives or flavorings, or as nutrients for bacterial culture media. They are often used to create savory flavors and umami taste sensations, and can be found in a large variety of packaged food, including frozen meals, crackers, snack foods, gravy, stock and more. They are rich in B vitamins (but not B12)
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The chemical compounds in beer give it a distinctive taste, smell and appearance. The majority of compounds in beer come from the metabolic activities of plants and yeast and so are covered by the fields of biochemistry and organic chemistry. The main exception is that beer contains over 90% water and the mineral ions in the water (hardness) can have a significant effect upon the taste
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Ethanol (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound with the chemical formula CH3CH2OH. It is an alcohol, with its formula also written as C2H5OH, C2H6O or EtOH, where Et stands for ethyl. Ethanol is a volatile, flammable, colorless liquid with a characteristic wine-like odor and pungent taste
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In biology, the BBCH-scale for coffee describes the phenological development of bean plants using the BBCH-scale. The phenological growth stages and BBCH-identification keys of coffee plants are: References Arcila-pulgarin, J. ; L
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Microfoam is finely textured milk used for making espresso-based coffee drinks, particularly those with latte art. It is typically made with the steam wand of an espresso machine, which pumps steam into a pitcher of milk. The opposite of microfoam is macrofoam (also called dry foam, in contrast to the wet foam of microfoam), which has visibly large bubbles, a style of milk commonly used for cappuccinos
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Trigonelline is an alkaloid with chemical formula C7H7NO2. It is a zwitterion formed by the methylation of the nitrogen atom of niacin (vitamin B3). Trigonelline is a product of niacin metabolism that is excreted in urine of mammals
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Pentosans are polymers composed of pentoses. In contrast to cellulose, which is composed of hexose (glucose) monomers, pentosans are derived from five-carbon sugars such as xylose. Pentosan-rich biomass is the precursor to furfural
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In chemistry, acid value (AV, acid number, neutralization number or acidity) is a number used to quantify the acidity of a given chemical substance. It is the quantity of base (usually potassium hydroxide (KOH)), expressed as milligrams of KOH required to neutralize the acidic constituents in 1 gram of a sample. The acid number is a measure of the number of carboxylic acid groups (−C(=O)OH) in a chemical compound, such as a fatty acid, or in a mixture of compounds
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p-Anisidine (or para-anisidine) is an organic compound with the formula CH3OC6H4NH2. A white solid, commercial samples can appear grey-brown owing to air oxidation. It is one of three isomers of anisidine, methoxy-containing anilines
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In liquids, the cloud point is the temperature below which a transparent solution undergoes either a liquid-liquid phase separation to form an emulsion or a liquid-solid phase transition to form either a stable sol or a suspension that settles a precipitate. The cloud point is analogous to the 'dew point' at which a gas-liquid phase transition called condensation occurs in water vapour (humid air) to form liquid water (dew or clouds). When the temperature is below 0 °C, the dew point is called the frost point, as water vapour undergoes gas-solid phase transition called deposition, solidification, or freezing
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In the analysis of the molecular formula of organic molecules, the degree of unsaturation (DU) (also known as the index of hydrogen deficiency (IHD), double bond equivalents (DBE), or unsaturation index) is a calculation that determines the total number of rings and π bonds. A formula is used in organic chemistry to help draw chemical structures. It does not give any information about those components individually—the specific number of rings, or of double bonds (one π bond each), or of triple bonds (two π bonds each)
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Fat hydrogenation is the process of combining unsaturated fat with hydrogen in order to partially or completely convert it into saturated fat. Typically this hydrogenation is done with liquid vegetable oils resulting in solid or semi-solid fats. Changing the degree of saturation of the fat changes some important physical properties, such as the melting range, which is why liquid oils become semi-solid
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In the food industry and biochemistry, interesterification (IE) is a process that rearranges the fatty acids of a fat product, typically a mixture of triglyceride. The process implies breaking and reforming the ester bonds C–O–C that connect the fatty acid chains to the glycerol hubs of the fat molecules. These reactions are performed by inorganic catalysts, yielding what is called chemical interesterification (CIE) in the industry; or by enzymes, in the so-called enzymatic interesterification (EIE)
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In chemistry, particularly in biochemistry, a fatty acid is a carboxylic acid with an aliphatic chain, which is either saturated or unsaturated. Most naturally occurring fatty acids have an unbranched chain of an even number of carbon atoms, from 4 to 28. Fatty acids are a major component of the lipids (up to 70% by weight) in some species such as microalgae but in some other organisms are not found in their standalone form, but instead exist as three main classes of esters: triglycerides, phospholipids, and cholesteryl esters
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In chemistry, the iodine value (IV; also iodine absorption value, iodine number or iodine index) is the mass of iodine in grams that is consumed by 100 grams of a chemical substance. Iodine numbers are often used to determine the degree of unsaturation in fats, oils and waxes. In fatty acids, unsaturation occurs mainly as double bonds which are very reactive towards halogens, the iodine in this case
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Detection of peroxide gives the initial evidence of rancidity in unsaturated fats and oils. Other methods are available, but peroxide value is the most widely used. It gives a measure of the extent to which an oil sample has undergone primary oxidation; extent of secondary oxidation may be determined from p-anisidine test
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Rancidification is the process of complete or incomplete autoxidation or hydrolysis of fats and oils when exposed to air, light, moisture, or bacterial action, producing short-chain aldehydes, ketones and free fatty acids. When these processes occur in food, undesirable odors and flavors can result. In processed meats, these flavors are collectively known as warmed-over flavor
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A saturated fat is a type of fat in which the fatty acid chains have all single bonds. A fat known as a glyceride is made of two kinds of smaller molecules: a short glycerol backbone and fatty acids that each contain a long linear or branched chain of carbon (C) atoms. Along the chain, some carbon atoms are linked by single bonds (-C-C-) and others are linked by double bonds (-C=C-)
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The smoke point, also referred to as the burning point, is the temperature at which an oil or fat begins to produce a continuous bluish smoke that becomes clearly visible, dependent upon specific and defined conditions. Smoke point values can vary greatly, depending on factors such as the volume of oil utilized, the size of the container, the presence of air currents, the type and source of light as well as the quality of the oil and its acidity content, otherwise known as free fatty acid (FFA) content. The more FFA an oil contains, the quicker it will break down and start smoking
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Trans fat, also called trans-unsaturated fatty acids, or trans fatty acids, is a type of unsaturated fat that occurs in foods. Trace concentrations of trans fats occur naturally, but large amounts are found in some processed foods. Since consumption of trans fats is unhealthy, artificial trans fats are highly regulated or banned in many nations
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An unsaturated fat is a fat or fatty acid in which there is at least one double bond within the fatty acid chain. A fatty acid chain is monounsaturated if it contains one double bond, and polyunsaturated if it contains more than one double bond. A saturated fat has no carbon to carbon double bonds, so the maximum possible number of hydrogens bonded to the carbons, and is "saturated" with hydrogen atoms
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In chemistry, the terms volatile acid (or volatile fatty acid (VFA)) and volatile acidity (VA) are used somewhat differently in various application areas. Wine In wine chemistry, the volatile acids are those that can be separated from wine through steam distillation. Many factors influence the level of VA, but the growth of spoilage bacteria and yeasts are the primary source and consequently VA is often used to quantify the degree of wine oxidation and spoilage
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The Babcock test is an inexpensive and practical procedure to determine the fat content of milk. It is named after its developer, Stephen M. Babcock (1843–1931), professor at the University of Wisconsin
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Degrees Brix (symbol °Bx) is a measure of the dissolved solids in a liquid, and is commonly used to measure dissolved sugar content of an aqueous solution. One degree Brix is 1 gram of sucrose in 100 grams of solution and represents the strength of the solution as percentage by mass. If the solution contains dissolved solids other than pure sucrose, then the °Bx only approximates the dissolved solid content
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In baking, a farinograph measures specific properties of flour. It was first developed and launched in 1928. The farinograph is a tool used for measuring the shear and viscosity of a mixture of flour and water
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Food energy is chemical energy that animals (including humans) derive from their food to sustain their metabolism, including their muscular activity. Most animals derive most of their energy from aerobic respiration, namely combining the carbohydrates, fats, and proteins with oxygen from air or dissolved in water. Other smaller components of the diet, such as organic acids, polyols, and ethanol (drinking alcohol) may contribute to the energy input
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Food moisture analysis is the determination of the concentration of water in a food sample. A variety of techniques may be used including Karl Fischer titration and loss on drying. Many technical standards exist which define test methods for determining moisture in different types of food
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The Kirschner value or Kirschner number is a value determined when examining fat. The Kirschner value is an indicator of how much volatile fatty acid can be extracted from fat through saponification. It consists of the number of milliliters of 0
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The Kjeldahl method or Kjeldahl digestion (Danish pronunciation: [ˈkʰelˌtɛˀl]) in analytical chemistry is a method for the quantitative determination of nitrogen contained in organic substances plus the nitrogen contained in the inorganic compounds ammonia and ammonium (NH3/NH4+). Without modification, other forms of inorganic nitrogen, for instance nitrate, are not included in this measurement. Using an empirical relation between Kjeldahl nitrogen content and protein content it is an important method for analyzing proteins
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Nima is a portable food sensor designed to enable individuals with food allergies and sensitivities to test their food for specific proteins. Nima created and leads the category of consumer food testers. The first product, a gluten sensor, was released in for general availability in February 2017
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The nitrogen solubility index (NSI) is a measure of the solubility of the protein in a substance. It is typically used as a quick measure of the functionality of a protein, for example to predict the ability of the protein to stabilise foams, emulsions or gels. To determine the NSI, the sample is dried, dispersed in a 0
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Free acidity is an important parameter that defines the quality of olive oil and is defined as a percentage as grams of free fatty acids (expressed as oleic acid, the main fatty acid present in olive oil) in 100 grams of oil. As defined by the European Commission regulation No. 2568/91 and subsequent amendments, the highest quality olive oil (extra-virgin olive oil) must feature a free acidity lower than 0
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PCR food testing is the engagement of polymerase chain reaction (PCR) technologies for the testing of food for the presence or absence of human pathogens, such as E. coli, Salmonella, Listeria, etc. Four sample collection sites for PCR food testing can be: The food irrigation water
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The Polenske value (also known as the Polenske number) is a value determined when examining fat. It is an indicator of how much volatile fatty acid can be extracted from fat through saponification. It is equal to the number of milliliters of 0
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A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from the observed refraction angle using Snell's law. For mixtures, the index of refraction then allows to determine the concentration using mixing rules such as the Gladstone–Dale relation and Lorentz–Lorenz equation
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A somatic cell count (SCC) is a cell count of somatic cells in a fluid specimen, usually milk. In dairying, the SCC is an indicator of the quality of milk—specifically, its low likeliness to contain harmful bacteria, and thus its high food safety. White blood cells (leukocytes) constitute the majority of somatic cells in question
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Canning is a method of food preservation in which food is processed and sealed in an airtight container (jars like Mason jars, and steel and tin cans). Canning provides a shelf life that typically ranges from one to five years, although under specific circumstances, it can be much longer. A freeze-dried canned product, such as canned dried lentils, could last as long as 30 years in an edible state
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Candwich is a canned food product created and produced by Mark One Foods, LLC (aka MK1 Foods). Candwich is marketed as "the only sandwich in a can. " Its slogan is, "Quick & Tasty, Ready to Eat"
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Canned tea is a relatively recent method of marketing tea which has been sold traditionally as leaf tea and also, for the last 100 years, in tea bag form. It utilises the canning process to produce a ready made drink. Perceived advantages includes ease of use (minimal or no preparation time) and the possibility of additives (such as flavours or sugar); the disadvantages are the cost of shipment (and therefore the price of the product) and a lack of freshness
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Canned tomatoes, or tinned tomatoes, are tomatoes, usually peeled, that are sealed into a can after having been processed by heat. Variants Canned tomatoes are available in several different forms. The traditional forms are whole peeled tomatoes, packed in juice or purée, and ground tomatoes, sometimes referred to as "kitchen-ready
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There are several species names which are called "triangulum"; "triangulum" is Latin referring to triangles Chaetodon triangulum (C. triangulum), the triangle butterflyfish, a species of fish Dendropsophus triangulum (D. triangulum), the triangle treefrog, a species of frog Eumorpha triangulum (E
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These are lists of invasive species by country or region. A species is regarded as invasive if it has been introduced by human action to a location, area, or region where it did not previously occur naturally (i. e
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The Cycles: International Grand Prix Racing is a 1989 computer game developed by Distinctive Software and published by Accolade. It has similarities to Grand Prix Circuit, except it is a motorcycle racing sim. The game includes all the tracks of 1989 Grand Prix motorcycle racing season
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Dark Fusion is a horizontally-scrolling platform shooter published by Gremlin Graphics in 1988. Levels are divided into three zones: Combat zone, Alien zone, and Flight zone. To enter a new zone, the player must first find a fusion pod
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Dragontorc is an action-adventure game developed by Steve Turner's Graftgold and released for the Amstrad CPC and ZX Spectrum by Hewson Consultants in 1985. It is a sequel to 1984's Avalon The 3D Adventure Movie. The hero of Avalon, Maroc the Mage, returns to defeat an evil witch and save Britain
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Dun Darach is an arcade adventure developed and published in 1985 by Gargoyle Games for the ZX Spectrum and Amstrad CPC computers. It is a prequel to the 1984 game Tir Na Nog. The plot has Celtic hero Cuchulainn on a search to find his companion Lóeg in the mysterious city of Dun Darach
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Elektra Glide is a futuristic motorcycle racing game developed by designer Adam Billyard for the Atari 8-bit, Amstrad CPC, and Commodore 64 home computers. It was published in 1985 by English Software. Mastertronic also published the game in the US
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Edith Layard Stephens (1884-1966) was a South African botanist, a leading authority on algae and fungi, particularly edible and poisonous mushrooms. Early life and education Stephens was born on December 6, 1884 in Cape Town, Cape Colony, as the daughter of Michael Stephens, who was a chief locomotive superintendent of the Cape Government Railways and Annie Hoskyn. In 1901, she matriculated at the Rustenburg School for Girls in Rondebosch, Cape Town
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AAON Inc. (Nasdaq: AAON) designs, manufactures and sells semi-custom heating, ventilation and air conditioning equipment (HVAC) for commercial and residential use. AAON Inc
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Baxi is part of BDR Thermea, one of Europe's largest manufacturers and distributors of domestic and commercial water and space heating systems. Baxi employs 6,400 people throughout Europe, with a turnover exceeding €1. 2 billion
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Chromalox, Inc. manufactures and supplies electric heat and control products for industrial heating applications in the U. S
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Currently known as Mitsubishi Electric Hydronics & IT Cooling Systems S. p. A
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Electronic Temperature Instruments Limited (ETI) is a worldwide manufacturer and distributor of thermometers and portable test and measurement instrumentation. ETI supplies catering, industrial, HVAC, wireless, legionnaires, refrigeration thermometers and environmental monitoring instruments to various industries. These include food production, chemical, pharmaceutical and construction services
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A gene drive is a natural process and technology of genetic engineering that propagates a particular suite of genes throughout a population by altering the probability that a specific allele will be transmitted to offspring (instead of the Mendelian 50% probability). Gene drives can arise through a variety of mechanisms. They have been proposed to provide an effective means of genetically modifying specific populations and entire species
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A guide RNA (gRNA) is a piece of RNA that functions as a guide for RNA- or DNA-targeting enzymes, with which it forms complexes. Very often these enzymes will delete, insert or otherwise alter the targeted RNA or DNA. They occur naturally, serving important functions, but can also be designed to be used for targeted editing, such as with CRISPR-Cas9 and CRISPR-Cas12
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Human germline engineering is the process by which the genome of an individual is edited in such a way that the change is heritable. This is achieved by altering the genes of the germ cells, which then mature into genetically modified eggs and sperm. For safety, ethical, and social reasons, there is broad agreement among the scientific community and the public that germline editing for reproduction is a red line that should not be crossed at this point in time
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LEAPER (Leveraging endogenous ADAR for programmable editing of RNA) is a genetic engineering technique in molecular biology by which RNA can be edited. The technique relies on engineered strands of RNA to recruit native ADAR enzymes to swap out different compounds in RNA. Developed by researchers at Peking University in 2019, the technique, some have claimed, is more efficient than the CRISPR gene editing technique
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Meganucleases are endodeoxyribonucleases characterized by a large recognition site (double-stranded DNA sequences of 12 to 40 base pairs); as a result this site generally occurs only once in any given genome. For example, the 18-base pair sequence recognized by the I-SceI meganuclease would on average require a genome twenty times the size of the human genome to be found once by chance (although sequences with a single mismatch occur about three times per human-sized genome). Meganucleases are therefore considered to be the most specific naturally occurring restriction enzymes
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No-SCAR genome editing is an editing method that is able to manipulate the Escherichia coli (E. coli) genome. The system relies on recombineering whereby DNA sequences are combined and manipulated through homologous recombination
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A recognition sequence is a DNA sequence to which a structural motif of a DNA-binding domain exhibits binding specificity. Recognition sequences are palindromes. The transcription factor Sp1 for example, binds the sequences 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3', where (G/T) indicates that the domain will bind a guanine or thymine at this position
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Surveyor nuclease assay is an enzyme mismatch cleavage assay used to detect single base mismatches or small insertions or deletions (indels). Surveyor nuclease is part of a family of mismatch-specific endonucleases that were discovered in celery (CEL nucleases). The enzyme recognizes all base substitutions and insertions/deletions, and cleaves the 3′ side of mismatched sites in both DNA strands with high specificityThis assay has been used to identify and analyze mutations in a variety of organisms and cell types, as well as to confirm genome modifications following genome editing (using CRISPR/TALENs/zinc fingers)
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Synthetic genome is a synthetically built genome whose formation involves either genetic modification on pre-existing life forms or artificial gene synthesis to create new DNA or entire lifeforms. The field that studies synthetic genomes is called synthetic genomics. Recombinant DNA technology Soon after the discovery of restriction endonucleases and ligases, the field of genetics began using these molecular tools to assemble artificial sequences from smaller fragments of synthetic or naturally occurring DNA
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Synthetic genomics is a nascent field of synthetic biology that uses aspects of genetic modification on pre-existing life forms, or artificial gene synthesis to create new DNA or entire lifeforms. Overview Synthetic genomics is unlike genetic modification in the sense that it does not use naturally occurring genes in its life forms. It may make use of custom designed base pair series, though in a more expanded and presently unrealized sense synthetic genomics could utilize genetic codes that are not composed of the two base pairs of DNA that are currently used by life
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TAL (transcription activator-like) effectors (often referred to as TALEs, but not to be confused with the three amino acid loop extension homeobox class of proteins) are proteins secreted by some β- and γ-proteobacteria. Most of these are Xanthomonads. Plant pathogenic Xanthomonas bacteria are especially known for TALEs, produced via their type III secretion system
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Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made by fusing a TAL effector DNA-binding domain to a DNA cleavage domain (a nuclease which cuts DNA strands). Transcription activator-like effectors (TALEs) can be engineered to bind to practically any desired DNA sequence, so when combined with a nuclease, DNA can be cut at specific locations
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Genome Research is a peer-reviewed scientific journal published by Cold Spring Harbor Laboratory Press. Disregarding review journals, Genome Research ranks 2nd in the category 'Genetics and Genomics' after Nature Genetics. The focus of the journal is on research that provides novel insights into the genome biology of all organisms, including advances in genomic medicine
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Genetically modified maize (corn) is a genetically modified crop. Specific maize strains have been genetically engineered to express agriculturally-desirable traits, including resistance to pests and to herbicides. Maize strains with both traits are now in use in multiple countries
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Bowman v. Monsanto Co. , 569 U
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Monsanto Co. v. Geertson Seed Farms, 561 U
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Monsanto Technology LLC v Cefetra BV and Others (2010) was a preliminary ruling by the European Court of Justice (ECJ) regarding the legal protection of biotechnological inventions. The case dealt with the interpretation of Article 9 of Directive 98/44/EC on the legal protection of biotechnological inventions, and it was the first ECJ interpretation of the 1998 directive. Facts Monsanto holds a European patent, EP 0 546 090, for a variety of soybean containing genes inserted into the plants DNA
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Orange petunias or A1-DFR petunias are genetically modified organisms which contain a transgene from maize that colors the petunia flowers orange. First created in a 1987 experiment at the Max Planck Institute for Plant Breeding Research in Cologne, the petunias were subsequently released into the wild but were not commercialized. In 2015 orange petunias were discovered in Helsinki by botanist Teemu Teeri, leading to a regulatory response dubbed the petunia carnage of 2017 in which plant sellers were directed to destroy the modified petunia plants rather than sell them
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Monsanto was involved in several high-profile lawsuits, as both plaintiff and defendant. It had been defendant in a number of lawsuits over health and environmental issues related to its products. Monsanto also made frequent use of the courts to defend its patents, particularly in the area of agricultural biotechnology
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The StarLink corn recalls occurred in the autumn of 2000, when over 300 food products were found to contain a genetically modified corn that had not been approved for human consumption. It was the first-ever recall of a genetically modified food. The anti-GMO activist coalition Genetically Engineered Food Alert, which detected and first reported the contamination, was critical of the FDA for not doing its job
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3 Hardman Street is a 16-storey high-rise building in Spinningfields, Manchester, England. At 75 m (246 ft), as of 2023 it is the third-tallest building in the Spinningfields area (after 1 Spinningfields and the Manchester Civil Justice Centre) and the joint 36th-tallest building in Greater Manchester. Background 3 Hardman Street was designed by architects Sheppard Robson, as part of the Allied London project regenerating Spinningfields into a major business centre
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