Datasets:

g19-f
/

g4-data

dataset_info:
  features:
    - name: image
      dtype: image
    - name: source
      dtype: string
  splits:
    - name: train
      num_bytes: 366173592
      num_examples: 120
  download_size: 366186996
  dataset_size: 366173592
configs:
  - config_name: default
    data_files:
      - split: train
        path: data/train-*
task_categories:
  - mask-generation
size_categories:
  - n<1K

Dataset Card

Dataset Summary

The dataset contains fluorescence microscopy images of cells stained with DAPI (Which stains the nucleus, channel 1) and CyclinB1 (which stains cytoplasm and nucleus, if it stains the nucleus it indicates the cell is in G2 or early prophase, channel 3). The goal of this project is to analyze whether Cyclin B1 is in the nucleus to determine where the cell is in the cell cycle.

Each cell has two corresponding TIFF images:

A DAPI image showing the nucleus
A CyclinB1 image showing cell cycle protein levels

The dataset was created for OPALS Group 19 project focused on understanding how CyclinB1 shows where the cell is in the cell cycle.

Data Fields

image — A PIL Image
source — The source of the data

Data Collection

Images were collected using fluorescence microscopy.
Cells were stained with:

DAPI to visualize nuclei
CyclinB1 to measure cell cycle progression

The dataset was prepared manually by organizing images and creating a JSON metadata file linking each cell’s channels.

Intended Uses

This dataset can be used for:

Cell segmentation
Cell cycle classification
Training or testing microscopy image models
Educational demonstrations of fluorescence imaging

Limitations

Small dataset size (fewer than 1,000 samples)
Only two fluorescence channels
No segmentation masks included
Images may vary slightly in brightness or focus

Citation

If you use this dataset, please cite the dataset creator or link to the Hugging Face dataset page.