title stringlengths 3 364 | abstract stringlengths 36 3.48k | claims stringlengths 67 94.4k | main_ipcr_label stringclasses 500
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METHODS AND COMPOSITIONS FOR TARGETED MUTAGENESIS IN BACTERIA | This disclosure provides methods and compositions for targeted mutagenesis of specific genes in a bacterial strain. By inducibly over-expressing error-prone polymerases such as Pol IV or Pol V in conjunction with nickase in a bacterial strain, and housing the targeted gene(s) on an episome or plasmid which contains one... | 1. A method of generating mutations within a target DNA comprising: providing a host bacterial strain, which comprises a nucleic acid coding for an error-prone DNA polymerase under control of an inducible transcriptional regulatory region, a nucleic acid coding for a nicking enzyme, and a plasmid vector comprising said... | C12N | 20120914 | 2,012 | 2012:175871 |
Compositions, Kits and Methods for Synthesis and/or Detection of Nucleic Acids | A composition comprising a thermostable DNA polymerase; and a PCR inhibitor blocking agent, wherein the PCR inhibitor blocking agent is present in an amount effective to enhance tolerance of an assembled PCR to a PCR inhibitor. | 1. A polymerase chain reaction (PCR) composition, comprising: a thermostable DNA polymerase; and a PCR inhibitor blocking agent, wherein the PCR inhibitor blocking agent is present in an amount effective to enhance tolerance of an assembled PCR to a PCR inhibitor. 2. The composition of claim 1, wherein the PCR inhibito... | C12N | 20110621 | 2,011 | 2011:104669 |
GENES THAT INCREASE PLANT OIL AND METHOD FOR USING THE SAME | This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the p... | 1. A plant which expresses a chimeric protein, said chimeric protein comprising a transcription factor fused to a repressor domain sequence that converts said transcription factor into a transcription repressor, wherein said transcription factor is selected from the group consisting of (a) and (b): (a) a protein compri... | C12N | 20140321 | 2,014 | 2014:48947 |
REGULATORY SEQUENCES TO CONTROL GENE EXPRESSION IN PLANTS | The present invention discloses regulatory sequences, promoters and terminators, and their use in plants. The regulatory sequences can be used to make gene constructs that include a gene not natively associated with the regulatory sequences. Methods to use the regulatory sequences with antisense constructs or functiona... | 1. A nucleic acid construct comprising a nucleic acid having promoter activity in a plant operably linked to a heterologous DNA of interest, wherein the nucleic acid having promoter activity in a plant is selected from the group consisting of: (a) a nucleic acid comprising nucleotides 1-1493 of SEQ ID NO:1; (b) a nucle... | C12N | 20131029 | 2,013 | 2013:226358 |
GLUCOAMYLASE VARIANTS | The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a correspondi... | 1-22. (canceled) 23. An isolated glucoamylase variant comprising a catalytic domain and a starch binding domain (SBD), a) said catalytic domain comprising at least 85% sequence identity to the amino acid sequence of SEQ ID NO:3 and b) said SBD comprising one or more amino acid substitutions at a position corresponding ... | C12N | 20130830 | 2,013 | 2013:168809 |
A Soluble and Stable Human 5-Lipoxygenase | A soluble and stable form of 5-lipoxygenase (5-LOX) has been made, 5-Lox is the enzyme which initiates leukotriene biosynthesis by catalyzing the two-step transformation of arachidomc acid to leukotriene A4 (LTA4). The soluble and stable 5-LOX is suitable for a number of applications, including, but not limited to, hig... | 1. An isolated 5-Lipoxygenase polypeptide comprising an amino acid sequence, numbered from the N-terminus, as set forth in SEQ ID NO:1 with one or more of the following modifications selected from the group comprising the following: (a) a replacement of amino acids Tryptophan and Phenylalanine at about residues 13-14 w... | C12N | 20130227 | 2,013 | 2013:2725 |
GENERAL COMPOSITION FRAMEWORK FOR LIGAND-CONTROLLED RNA REGULATORY SYSTEMS | The invention provides an improved design for the construction of extensible nucleic acid-based, ligand-controlled regulatory systems, and the nucleic acid regulatory systems resulting therefrom. The invention contemplates improving the design of the switches (ligand-controlled regulatory systems) through the design of... | 1-21. (canceled) 22. A method for regulating expression of a recombinant gene, comprising: (i) providing a cell of claim 21, (ii) contacting the cell with said molecule in an amount that alters the activity of said modular actuator domain. 23-25. (canceled) 26. A method for rendering expression of a target gene in a ce... | C12N | 20120815 | 2,012 | 2012:146089 |
METHODS FOR MANUFACTURING PLANT CELL WALLS COMPRISING CHITIN | Methods and means are provided for the modification of the reactivity of plant secondary cell walls, particularly in cotton cell walls found in cotton fibers. This can be conveniently achieved by expressing a chimeric gene encoding a Saprolegnia monoica chitin synthase in cotton plants. | 1. An isolated nucleic acid molecule comprising a nucleotide sequence which encodes a chitin synthase polypeptide wherein said nucleotide sequence is at least 97% identical to SEQ ID NO: 1 or a complementary sequence thereof. 2. An isolated nucleic acid molecule comprising a nucleotide sequence which encodes a chitin s... | C12N | 20121109 | 2,012 | 2012:107848 |
REGULATING ALKALOIDS | MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in Nicotiana plants. In particular, suppressing or overexpressing one or more of MPO1 and MPO2 may be used to decrease or increase nicotine and nicotinic alkaloid levels in tobacco plants. Suppression or overexpr... | 1.-16. (canceled) 17. An N-methylputrescine oxidase-1 (MPO1) polypeptide having the amino acid sequence of SEQ ID NO: 2 18. An N-methylputrescine oxidase-2 (MPO2) polypeptide having the amino acid sequence of SEQ ID NO: 4 19. (canceled) 20. A method of producing an N-methylputrescine oxidase (MPO) enzyme, comprising: (... | C12N | 20150810 | 2,015 | 2015:136437 |
Interleukin-1 Alpha Antibodies and Methods of Use | Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1α and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors. | 1. A method of inhibiting migration of a cell through a basement membrane matrix, the method comprising the step of adding a purified mAb that specifically binds to human IL-1a to a mixture comprising the basement membrane matrix and the cell. 2. The method of claim 1, wherein the purified mAb is a human IgG1 mAb compr... | C12N | 20110902 | 2,011 | 2011:139120 |
HYDROXYNITRILE LYASE | An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per ... | 1-12. (canceled) 13. An isolated or purified modified hydroxynitrile lyase that is at least 90% homologous to the wild-type hydroxynitrile lyase of Manihot escuela of SEQ ID NO: 1 or at least 90% homologous to the wild-type hydroxynitrile lyase of Hevea brasiliensis of SEQ ID NO: 102, and that has a substitution of at ... | C12N | 20110816 | 2,011 | 2011:130206 |
DNA PROMOTERS AND ANTRHAX VACCINES | The invention is related to intracellularly induced bacterial DNA promoters and vaccines against Bacillus anthracis. | 1. An attenuated bacterium for the expression of a heterologous protein, the expression of the heterologous protein being under the control of a promoter, wherein the promoter is selected from the group consisting of the promoters of SEQ ID NOs: 1-8 and functional fractions thereof. 2. The attenuated bacterium of claim... | C12N | 20120717 | 2,012 | 2012:125306 |
NUCLEIC ACID/POLYSACCHARIDE COMPLEX | An object of the present invention is to provide a highly stable nucleic acid-polysaccharide complex of an siRNA and schizophyllan. A nucleic acid-polysaccharide complex is formed by adding polydeoxyadenine in which at least part of the phosphodiester link portion is phosphorothioated to an siRNA and allowing the siRNA... | 1. A pharmaceutical preparation for suppressing rejection occurring in transplantation therapy, comprising a nucleic acid-polysaccharide complex of schizophyllan and a polynucleotide containing an siRNA to which polydeoxyadenine is added, against a costimulatory factor. 2. The pharmaceutical preparation according to cl... | C12N | 20131028 | 2,013 | 2013:160674 |
PON POLYPEPTIDES, POLYNUCLEOTIDES ENCODING SAME AND COMPOSITIONS AND METHODS UTILIZING SAME | Isolated polynucleotides and polypeptides encoded therefrom are provided. These include mutated PON enzymes with increased, modified or substantially the same substrate specificity as compared to respective wild-type PON. Also provided are kits and methods using these enzymes. | 1. An isolated polypeptide comprising an amino acid sequence of a mutated serum paraoxonase (PON1) enzyme at least 95% identical to SEQ ID NO: 56, said mutated PON1 enzyme being characterized by: (i) a phosphotriester hydrolytic activity and phosphotriesterase substrate specificity; and (ii) no substantial formation of... | C12N | 20131021 | 2,013 | 2013:193744 |
DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION | Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells... | 1. A DNA polymerase having increased 3′-mismatch discrimination activity compared with a control DNA polymerase, wherein the amino acid of the DNA polymerase corresponding to position 752 of SEQ ID NO:1 is any amino acid other than N, and wherein the control DNA polymerase has the same amino acid sequence as the DNA po... | C12N | 20110711 | 2,011 | 2011:113516 |
Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same | The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 1-48. (canceled) 49. An isolated polypeptide having cellobiohydrolase activity, selected from the group consisting of: (a) a polypeptide having at least 68% sequence identity to the mature polypeptide of SEQ ID NO: 2, or a polypeptide having at least 68% sequence identity to the mature polypeptide of SEQ ID NO: 4, or a... | C12N | 20130624 | 2,013 | 2013:117096 |
MODULATION OF DYSTROPHIA MYOTONICA-PROTEIN KINASE (DMPK) EXPRESSION | Provided herein are methods, compounds, and compositions for reducing expression of a DMPK mRNA and protein in an animal. Also provided herein are methods, compounds, and compositions for preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal. Such methods, compounds, and compo... | 1. A method of treating an animal having type 1 myotonic dystrophy comprising administering to the animal a therapeutically effective amount of a compound comprising an oligonucleotide consisting of 10 to 30 linked nucleosides targeted to a DMPK nucleic acid, wherein the oligonucleotide has a nucleobase sequence that i... | C12N | 20150730 | 2,015 | 2015:132710 |
Hepatic Lobule-Like Bioreactor | The present invention provides a hepatic lobule-like bioreactor. The bioreactor includes a nanofiber scaffold enclosed within a housing. An intrahepatic fibrous vascular network, a bile capillary network, upper hepatic bile ducts, lower hepatic bile ducts, a common bile duct connecting the upper and the lower hepatic b... | 1. A hepatic lobule-like bioreactor, comprising: a closed-housing; a nanofiber scaffold enclosed within the housing; an intrahepatic fibrous vascular network where metabolism and material exchange take place, the intrahepatic fibrous vascular network provided with a liquid inlet port and a liquid outlet port; a bile ca... | C12N | 20131206 | 2,013 | 2013:163027 |
CELL CULTURING METHOD, CELL CULTURING APPARATUS AND KIT | The present invention relates to a cell culturing method as well as a cell culturing apparatus and kit for use in said culturing method. This cell culturing method comprises applying cells on a porous polyimide film and culturing. One embodiment of the method according to the present invention comprises a process for s... | 1. A cell culturing method that includes applying cells to a porous polyimide film and culturing them. 2. The method according to claim 1, including a step of seeding cells on the surface of a porous polyimide film. 3. The method according to claim 1, including a step of: placing a cell suspension on the dried surface ... | C12N | 20160125 | 2,016 | 2016:2178 |
METHODS FOR TREATING NIEMANN-PICK TYPE C DISEASE | The present invention includes methods for treating Niemann-Pick Type C disease through administration of inhibitors of acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1). ACAT inhibitors are used to treat symptoms of Niemann-Pick Type C disease and prolong survival of patients with the disease, either alone or in c... | 1. A method for treating Niemann-Pick Type C disease comprising administering to a patient diagnosed with Niemann-Pick Type C disease an effective amount of an Acyl-CoA:Cholesterol Acyltransferase (ACAT) inhibitor in a pharmaceutically acceptable vehicle. 2. The method of claim 1, wherein the ACAT inhibitor is administ... | C12N | 20140326 | 2,014 | 2014:56806 |
Manufacturing Method of Immune Killer Cells | The present invention relates to a manufacturing method of immune killer cells characterized in that an immune killer cell is induced in a culture medium containing concanavalin A (ConA), and the immune killer cell is maintained or expanded in a culture procedure. Antibody proteins are not used as a stimulant in the cu... | 1. A manufacturing method of immune killer cells, comprising steps of having an immune killer cell is induced in a culture medium containing concanavalin A (ConA), and the immune killer cell is maintained or expanded a culture procedure. 2. The manufacturing method of immune killer cells according to claim 1, wherein t... | C12N | 20110209 | 2,011 | 2011:24047 |
Compositions and Methods for Reducing Background DNA Amplification | Compositions are provided that include a plurality of small molecules selected from the group consisting of an amide, urea or acetone having a molecular weight less than 300 g/mol; and dNTPs and a polymerase in a buffer suitable for use as an amplification buffer. Methods of use of the compositions are also described f... | 1. A composition comprising a plurality of small molecules selected from the group consisting of an amide, urea or acetone having a molecular weight less than 300 g/mol; and dNTPs and a polymerase in a buffer suitable for use as a DNA amplification buffer. 2. A composition according to claim 1 wherein the plurality of ... | C12N | 20130313 | 2,013 | 2013:44102 |
DOUBLE STRANDED RNA CONSTRUCTS FOR APHID CONTROL | Disclosed are specific aphid dsRNA constructs that target either Chloride Intracellular Channel (CLIC) gene expression or Sucrase gene expression. Also disclosed is the use of dsRNA constructs of a CLIC gene to interfere with critical functions of CLIC gene peptide products. A novel method to develop nucleic acid contr... | 1. A double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a chloride intracellular channel protein (CLIC) in a cell, wherein said dsRNA comprises a sense strand comprising a first sequence and an antisense stand comprising a second sequence complementary to SEQ ID NO: 5, wherein said first sequence... | C12N | 20150813 | 2,015 | 2015:138066 |
Oligomers | Certain disclosed oligomers induce exon skipping during processing of myostatin pre-mRNA. The oligomers may be in a vector or encoded by the vector. The vector is used for inducing exon skipping during processing of myostatin pre-mRNA. A therapeutically effective amount of the oligomer may be administered to a subject ... | 1. An oligomer for inducing exon skipping during processing of myostatin pre-mRNA, the oligomer comprising at least 20 contiguous bases of a base sequence selected from the group consisting of: (SEQ ID NO. 1) 1) XCXCGACGGGXCXCAAAXAXAXCCAXAGXX; (SEQ ID NO. 2) 2) XGXACCGXCXXXCAXAGGXXXGAXGAGXCX; (SEQ ID NO. 3) 3) CCXGGGXX... | C12N | 20141002 | 2,014 | 2014:191168 |
BACULOVIRUS SYSTEM FOR THE EXPRESSION OF A GENE THERAPY VECTOR | The invention concerns a recombinant baculovirus genome useful for the expression of gene therapy vectors by means of a single infection. | 1. A recombinant baculovirus genome comprising one or more expression cassettes for the protein components required for the production of a heterologous viral vector, and a recombinant genome of a heterologous viral vector, said expression cassettes and said recombinant genome of a heterologous viral vector being inser... | C12N | 20140623 | 2,014 | 2014:56498 |
FLOW CYTOMETER METHOD AND APPARATUS | A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electro... | 1-80. (canceled) 81. A nozzle for use in flow cytometry apparatus for analyzing particles in a fluid stream, said fluid stream comprising a sheath stream surrounding a core stream containing said particles, said nozzle comprising: a nozzle having an interior surface defining a flow path for said fluid stream, and an or... | C12N | 20130301 | 2,013 | 2013:33907 |
METHOD FOR PRODUCING HEMATOPOIETIC STEM CELLS USING PYRAZOLE COMPOUNDS | An expanding agent for hematopoietic stem cells and/or hematopoietic progenitor cells useful as a therapy for various hematopoietic diseases and useful for improvement in the efficiency of gene transfer into hematopoietic stem cells for gene therapy is provided. A method of producing hematopoietic stem cells and/or hem... | 1: A method of producing hematopoietic stem cells and/or hematopoietic progenitor cells, the method comprising expanding hematopoietic stem cells and/or hematopoietic progenitor cells by culturing hematopoietic stem cells ex vivo in the presence of a pyrazole compound represented by the formula (1): wherein: R1 is a hy... | C12N | 20131104 | 2,013 | 2013:155351 |
METHODS FOR RAPIDLY TRANSFORMING MONOCOTS | The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method... | 1. A method for producing a transgenic corn plant comprising: a) transforming an explant with at least a first selected DNA via bacterially mediated transformation of the explant; b) culturing the explant in a first culture medium comprising an effective ratio of cytokinin and auxin to promote development of regenerabl... | C12N | 20140911 | 2,014 | 2014:179662 |
MUTATED REP ENCODING SEQUENCES FOR USE IN AAV PRODUCTION | The invention relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein a nuclear localization signal (NLS) in said Parvoviral Rep protein is mutated as compared with a corresponding wild type sequence. The invention also relates to a nucleic acid comprising a nucleotide sequ... | 1. A nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein a nuclear localization signal (NLS) in said Parvoviral Rep protein is mutated as compared with a corresponding wild type sequence. 2. The nucleic acid according to claim 1, wherein the NLS is at least partially truncated and/o... | C12N | 20120910 | 2,012 | 2012:144885 |
Stabilization Of Perhydrolases | Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powd... | 1. A process to stabilize the perhydrolysis activity of an enzyme when present in a formulation comprised of said enzyme and a carboxylic acid ester, the process comprising: (a) providing an aqueous formulation comprising: (i) at least one enzyme structurally classified as a CE-7 enzyme and having perhydrolysis activit... | C12N | 20120712 | 2,012 | 2012:123089 |
METHOD OF ISOLATING NUCLEIC ACID FROM SPECIMENS IN LIQUID-BASED CYTOLOGY PRESERVATIVES CONTAINING FORMALDEHYDE | Method, composition, kit and system for isolating amplifiable nucleic acid from specimens preserved in a liquid-based cytology preservative that contains formaldehyde. The technique relies on the use of 2-imidazolidone and a protease enzyme, such as proteinase K, at elevated temperatures. Advantageously, RNA can be iso... | 1. A method of processing a specimen that includes a clinical sample disposed in a liquid-based cytology preservative that comprises formaldehyde, the method comprising the steps of: (a) combining the specimen with a protease enzyme and formaldehyde scavenger to create a reaction mixture; (b) incubating the reaction mi... | C12N | 20150302 | 2,015 | 2015:44188 |
CULTURE MEDIUM | A culture medium is used for culturing neural cells. Each neural cell includes a neural cell body and at least one neurite branched from the neural cell body. The culture medium includes a substrate and a carbon nanotube structure located on the substrate. The carbon nanotube structure includes a number of carbon nanot... | 1. A culture medium for culturing neural cells, each neural cell comprising a neural cell body and at least one neurite branched from the neural cell body, the culture medium comprising: a substrate; and a carbon nanotube structure located on the substrate, the carbon nanotube structure comprising a plurality of carbon... | C12N | 20120801 | 2,012 | 2012:133193 |
MESENCHYMAL-LIKE STEM CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS, METHODS AND USES THEREOF | The present invention relates to methods of generating and expanding hitman embryonic stem eel! derived mesenchymal-like stem/siromal cells. These hES-MSCs are characterized at least in part by the low level of expression of IL-6. These cells are useful for the prevention and treatment of T cell related autoimmune dise... | 1. A method for producing human embryonic stem cell-derived mesenchymal stem cells, comprising: a. culturing human embryonic stem cells with serum free media with or without GSK3 inhibitors; b. culturing human embryonic stem cells in serum-free media comprising vascular endothelial growth and bone morphogenic protein 4... | C12N | 20150107 | 2,015 | 2015:1476 |
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