| ### 1. CellProfiler-Training (12 training modules) |
| Educational content with problem statements and guided solutions: |
| - **ExampleColocalization**: Colocalization training |
| - **ExampleCometAssay**: Comet assay training |
| - **ExampleImagingFlowCytometryObjectsInGrid**: Flow cytometry training |
| - **ExamplePercentPositive**: Percentage analysis training |
| - **ExampleTrackObjects**: Object tracking training |
| - **ExampleWoundHealing**: Wound healing training |
| - **Tutorial3DFISH**: 3D FISH analysis |
| - **Tutorial3DMonolayer**: 3D monolayer analysis |
| - **Tutorial3DNoiseNuclei**: 3D nuclei with noise |
| - **TutorialBeginnerSegmentation**: Basic segmentation |
| - **TutorialTranslocation**: Protein translocation analysis |
| - **TutorialUnmix**: Color deconvolution |
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| ### 5. CellProfiler-Testing (14 test challenges) |
| Testing scenarios for algorithm development and validation: |
| - Various morphological analysis challenges |
| - Segmentation challenges for different cell types |
| - Colocalization testing |
| - Speckle counting validation |
| - Multi-nuclei segmentation tests |
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