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42481854
10.1038/s41591-026-04549-6
An iPSC-derived neural progenitor cell therapy for subacute spinal cord injury: a phase 1 trial with long-term follow-up
Spinal cord injury (SCI) causes irreversible motor and sensory deficits, and no therapy currently restores the damaged neural circuitry. Previous work has shown that transplantation of induced pluripotent stem cell (iPSC)-derived neural stem/progenitor cells (NS/PCs) restored motor function in preclinical models of sub...
Nat Med
Nature medicine
2026 Jul 21
2,026
[ "Sugai K", "Tsuji O", "Fujiyoshi K", "Ozaki M", "Abe T", "Kohzuki T", "Okawara H", "Sawada T", "Sumida M", "Yoshioka E", "Shofuda T", "Kawashima S", "Yamaguchi R", "Shinozaki M", "Takasu N", "Nagoshi N", "Okubo T", "Kohyama J", "Maeda T", "Yato Y", "Kanemura Y", "Yamanaka S...
Sugai K
Okano H
[]
[ "Journal Article" ]
null
null
null
null
1078-8956
eng
https://pubmed.ncbi.nlm.nih.gov/42481854/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42455434
10.1007/978-981-92-1439-6_1
Gonocytes in Transition: Establishing the Male Germline Identity
Male germline development is characterized by a unique perinatal phase in which primordial germ cells (PGCs) differentiate into gonocytes (prospermatogonia) and subsequently give rise to spermatogonia. This chapter outlines the cellular, molecular, and epigenetic programs that orchestrate this transition, establishing ...
Adv Exp Med Biol
Advances in experimental medicine and biology
2026
2,026
[ "Li P", "Ohhata T", "Sakai S", "Niida H", "Yamanaka S" ]
Li P
Yamanaka S
[ "Animals", "Male", "Humans", "Epigenesis, Genetic", "DNA Methylation", "Spermatogenesis", "Spermatogonia", "Cell Differentiation", "Signal Transduction", "Gene Expression Regulation, Developmental", "Germ Cells" ]
[ "Journal Article", "Review" ]
1517
null
3-16
null
0065-2598
eng
https://pubmed.ncbi.nlm.nih.gov/42455434/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42350164
10.1016/j.eururo.2026.05.016
Re: Xenotransplantation of a Porcine Kidney for End-stage Kidney Disease
null
Eur Urol
European urology
2026 Jun 26
2,026
[ "Yamamoto S", "Yamanaka S", "Yokoo T", "Kimura T", "Egawa S" ]
Yamamoto S
Egawa S
[]
[ "Journal Article" ]
null
null
null
null
0302-2838
eng
https://pubmed.ncbi.nlm.nih.gov/42350164/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42342341
10.1253/circj.CJ-26-0380
Rupture of a Splenic Artery Aneurysm After Thrombolysis Mimicking a Pancreatic Cyst
null
Circ J
Circulation journal : official journal of the Japanese Circulation Society
2026 Jul 24
2,026
[ "Kobayashi N", "Tokuyama H", "Yamanaka S", "Kanamori T", "Sato N" ]
Kobayashi N
Sato N
[]
[ "Journal Article" ]
90
8
1113
null
1346-9843
eng
https://pubmed.ncbi.nlm.nih.gov/42342341/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42283672
10.1016/j.jaccas.2026.108798
Progressive Giant Left Atrial Appendage Thrombus After Aborted Appendage Occlusion Despite Anticoagulation
Thrombus formation after successful left atrial appendage occlusion (LAAO) has been well described; however, the clinical course after aborted LAAO remains poorly characterized. An 84-year-old man with atrial fibrillation and a history of cardioembolic events underwent an attempted LAAO procedure that was aborted becau...
JACC Case Rep
JACC. Case reports
2026 Jun 12
2,026
[ "Kawahara R", "Tokuyama H", "Yamanaka S", "Yui Y", "Kobayashi N", "Ota H", "Miyauchi Y" ]
Kawahara R
Miyauchi Y
[]
[ "Case Reports", "Journal Article" ]
null
null
108798
null
2666-0849
eng
https://pubmed.ncbi.nlm.nih.gov/42283672/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42281563
10.1002/jgf2.70142
Effect of a Clinical Decision Support System on Team-Based Clinical Quiz Performance Among Japanese Medical Students: A Pre-Post Pilot Study
Educational impact of point-of-care clinical decision support system (CDSS) on medical students remains insufficiently studied. Our aim was to evaluate the effect of a CDSS on team-based clinical quiz performance and confidence in using medical information resources. We conducted a nationwide, online, pre-post quasi-ex...
J Gen Fam Med
Journal of general and family medicine
2026 Jul
2,026
[ "Isoda S", "Hashimoto T", "Nishizawa T", "Sekiya S", "Yamanaka S", "Misawa M", "Watari T", "Ueda E", "Suzuki T", "Tokuda Y" ]
Isoda S
Tokuda Y
[]
[ "Journal Article" ]
27
4
e70142
pmc-id: PMC13250386;
2189-6577
eng
https://pubmed.ncbi.nlm.nih.gov/42281563/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42267589
10.1080/17501911.2026.2685055
Transposable element regulation in mammalian germ cells and other contexts: multilayered repression, fertility, and regulated activation
Transposable elements (TEs), particularly retrotransposons that dominate mammalian genomes, are pervasive components of mammalian genomes whose activation is constrained by multilayered repression systems. In germ cells, this repression architecture is particularly elaborate, integrating chromatin-based silencing, DNA ...
Epigenomics
Epigenomics
2026 Jul
2,026
[ "Li P", "Yamanaka S" ]
Li P
Yamanaka S
[ "Animals", "DNA Transposable Elements", "Germ Cells", "Humans", "Epigenesis, Genetic", "Mammals", "Fertility", "DNA Methylation", "Gene Expression Regulation, Developmental", "Chromatin Assembly and Disassembly" ]
[ "Journal Article", "Review" ]
18
7
843-860
pmc-id: PMC13390519;
1750-1911
eng
https://pubmed.ncbi.nlm.nih.gov/42267589/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42166078
10.1007/s10014-026-00540-8
Dual TERT promoter mutations in glioblastoma, IDH-wildtype: a case report with preclinical investigations
Telomerase reverse transcriptase promoter (TERTp) mutations c.-124C > T (C228T) and c.-146C > T (C250T) are the most frequent single-nucleotide variants promoting telomerase activation in glioblastoma. Here, we describe a rare case of glioblastoma IDH-wildtype with dual TERTp mutations. Genomic analyses of multiregiona...
Brain Tumor Pathol
Brain tumor pathology
2026 May 21
2,026
[ "Miyake Y", "Tateishi K", "Okagawa H", "Koyama A", "Hirata E", "Sasaoka K", "Saito S", "Fushimi S", "Natsumeda M", "Muraoka E", "Yamanaka S", "Fujii S", "Yamamoto T" ]
Miyake Y
Yamamoto T
[]
[ "Journal Article" ]
null
null
null
null
1433-7398
eng
https://pubmed.ncbi.nlm.nih.gov/42166078/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42083033
10.1186/s13027-026-00765-4
Absence of chlamydial infection in ocular adnexal MALT lymphoma based on next-generation sequencing
Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT) arises in various extranodal sites and has been linked to chronic inflammation, often triggered by infectious agents. Ocular adnexal (OA) MALT lymphoma has been associated with Chlamydophila psittaci (C. psittaci) in some regions. This study ...
Infect Agent Cancer
Infectious agents and cancer
2026 May 4
2,026
[ "Yakushijin Y", "Horiba K", "Hashino M", "Yamanaka S", "Hasebe S", "Yamamoto S", "Fujii T", "Takenaka K", "Yasukawa M" ]
Yakushijin Y
Yasukawa M
[]
[ "Journal Article" ]
21
1
null
pmc-id: PMC13317347;
1750-9378
eng
https://pubmed.ncbi.nlm.nih.gov/42083033/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42066769
10.1016/j.stem.2026.04.006
eIF4G2-mediated selective translation of chromatin regulators safeguards adult intestinal stem cell identity and differentiation
eIF4G2 (DAP5/NAT1) is a non-canonical translation initiation factor, but its role in homeostasis is unclear. Using inducible Eif4g2 knockout mice and intestinal organoids, we show that eIF4G2 loss collapses Lgr5+ intestinal stem cell (ISC) and secretory maturation programs while preserving villus architecture. Transcri...
Cell Stem Cell
Cell stem cell
2026 Jun 4
2,026
[ "Kunitomi H", "Khaine AM", "Jamee R", "Arreola V", "Lancero M", "Raychaudhuri A", "Perli S", "Sato Y", "Iwasaki M", "Ruivo P", "Tomoda K", "Mito M", "Shichino Y", "Iwasaki S", "Yamanaka S" ]
Kunitomi H
Yamanaka S
[ "Animals", "Cell Differentiation", "Intestines", "Mice", "Chromatin", "Eukaryotic Initiation Factor-4G", "Adult Stem Cells", "Protein Biosynthesis", "Mice, Knockout", "Histones", "E1A-Associated p300 Protein" ]
[ "Journal Article" ]
33
6
1000-1015.e11
null
1875-9777
eng
https://pubmed.ncbi.nlm.nih.gov/42066769/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42008500
10.1371/journal.pone.0346972
Orthognathic surgery and postoperative nausea and vomiting: An 11-year retrospective observational study
Orthognathic surgery (OGS) is now widely performed, and postoperative nausea and vomiting (PONV) remains a common complication. Gastric decompression using a nasogastric tube (NT) is generally considered an effective prophylactic measure. In this retrospective observational study, we reviewed 667 OGS cases in 632 patie...
PLoS One
PloS one
2026
2,026
[ "Watanabe T", "Uozumi R", "Kawamura T", "Sasaki M", "Okada R", "Inoue S", "Kashiwagi M", "Fukuhara S", "Yamanaka S", "Mishima S", "Yamaguchi A", "Hirota M" ]
Watanabe T
Hirota M
[ "Humans", "Female", "Retrospective Studies", "Postoperative Nausea and Vomiting", "Adult", "Male", "Young Adult", "Orthognathic Surgical Procedures", "Middle Aged", "Adolescent", "Intubation, Gastrointestinal" ]
[ "Journal Article", "Observational Study" ]
21
4
e0346972
pmc-id: PMC13095023;
1932-6203
eng
https://pubmed.ncbi.nlm.nih.gov/42008500/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
42003820
10.1002/nau.70295
Activities of Daily Living Level is Associated With Recovery of Catheter-Free Urination Among Patients Hospitalized in an Acute Care Hospital
To examine the Activities of Daily Living (ADL) level required to achieve catheter-free urination in cases where a urinary catheter was inserted due to urinary retention, urination difficulty, or urinary incontinence in an acute care hospital. This study included patients who were originally capable of self-urination b...
Neurourol Urodyn
Neurourology and urodynamics
2026 Aug
2,026
[ "Tanaka T", "Kanematsu A", "Nagai N", "Yoneda H", "Yamanaka S", "Kou Y", "Yanagi T", "Yamada Y", "Saito R", "Domen K", "Yamamoto S" ]
Tanaka T
Yamamoto S
[ "Humans", "Female", "Male", "Activities of Daily Living", "Urinary Catheterization", "Recovery of Function", "Aged", "Aged, 80 and over", "Middle Aged", "Urination", "Urinary Retention", "Hospitalization", "ROC Curve", "Treatment Outcome", "Urinary Catheters", "Urinary Incontinence", ...
[ "Journal Article" ]
45
6
1196-1201
pmc-id: PMC13387999;
0733-2467
eng
https://pubmed.ncbi.nlm.nih.gov/42003820/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41969545
10.47371/mycosci.2025.04.002
Effects of different mycorrhizal types of dispersedly retained trees on the diversity of ectomycorrhizal fungi in neighboring Abies sachalinensis seedlings
Retention forestry conserves biodiversity by retaining forest structures in logged areas. It has been demonstrated that dispersedly retained broad-leaved ectomycorrhizal (EcM) trees can mitigate the effect of logging on the diversity of EcM fungi in the surrounding Abies sachalinensis seedlings. However, it remains unc...
Mycoscience
Mycoscience
2025
2,025
[ "Obase K", "Yamanaka S" ]
Obase K
Yamanaka S
[]
[ "Journal Article" ]
66
3
195-200
pmc-id: PMC13062905;
1340-3540
eng
https://pubmed.ncbi.nlm.nih.gov/41969545/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41959091
10.64898/2026.03.30.715396
Temporal AI model predicts drivers of cell state trajectories across human aging
Foundational AI models have recently shown promise for predicting the impact of perturbations on cell states. However, current models typically consider only one cell state at a time, limiting their ability to learn how cellular responses unfold over time, particularly across long trajectories such as diseases of aging...
bioRxiv
bioRxiv : the preprint server for biology
2026 Apr 1
2,026
[ "Ortega JG", "Nadadur RD", "Kunitomi A", "Kothen-Hill S", "Wagner JUG", "Kurtoglu SD", "Kim B", "Reid MM", "Lu T", "Washizu K", "Zanders L", "Chen H", "Zhang Y", "Ancheta S", "Lichtarge S", "Johnson WA", "Thompson C", "Phan DM", "Combes AJ", "Yang AC", "Tadimeti N", "Dimmel...
Ortega JG
Theodoris CV
[]
[ "Journal Article", "Preprint" ]
null
null
null
pmc-id: PMC13060336;
2692-8205
eng
https://pubmed.ncbi.nlm.nih.gov/41959091/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41947481
10.1111/jog.70270
Combined Large Cell Neuroendocrine Carcinoma of the Endometrium Treated With Paclitaxel, Carboplatin, and Durvalumab, Followed by Maintenance Therapy With Durvalumab and Olaparib (DUO-E Regimen): A Case Report
Combined large cell neuroendocrine carcinoma (LCNEC) of the endometrium is rare and carries a poor prognosis. A 61-year-old woman with suspected endometrial serous carcinoma was referred to our hospital. Imaging studies staged the disease as stage IIIC1. We performed a total hysterectomy, bilateral salpingo-oophorectom...
J Obstet Gynaecol Res
The journal of obstetrics and gynaecology research
2026 Apr
2,026
[ "Suzuki T", "Sonehara K", "Nabeshima H", "Shioya Y", "Yamanaka S", "Kondo S" ]
Suzuki T
Kondo S
[ "Humans", "Female", "Endometrial Neoplasms", "Middle Aged", "Carboplatin", "Carcinoma, Neuroendocrine", "Paclitaxel", "Phthalazines", "Antineoplastic Combined Chemotherapy Protocols", "Piperazines", "Antibodies, Monoclonal", "Carcinoma, Large Cell", "Maintenance Chemotherapy" ]
[ "Case Reports", "Journal Article" ]
52
4
e70270
null
1341-8076
eng
https://pubmed.ncbi.nlm.nih.gov/41947481/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41938366
10.2147/IJGM.S559679
Factors Associated and Survival Outcomes Among Japanese Patients with Solid Cancer Who Developed Deep Vein Thrombosis (DVT)
Deep vein thrombosis (DVT) is a common and serious complication in patients with cancer. We retrospectively analyzed patients newly diagnosed with solid cancers in a Japanese cohort. We retrospectively reviewed the medical records (2013-2020) of Japanese patients with solid cancers who were suspected of having DVT and ...
Int J Gen Med
International journal of general medicine
2026
2,026
[ "Masuda Y", "Yakushijin Y", "Yamanaka S", "Hasebe S", "Fujii T", "Yamanouchi J", "Takenaka K" ]
Masuda Y
Takenaka K
[]
[ "Journal Article" ]
19
null
559679
pmc-id: PMC13047771;
1178-7074
eng
https://pubmed.ncbi.nlm.nih.gov/41938366/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41932313
10.1016/j.molcel.2026.03.004
An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation
The DDB1- and CUL4-associated factor (DCAF) family functions as substrate receptors within Cullin4-really interesting new gene (RING) ubiquitin ligases (CRL4s), facilitating proteasomal degradation of targeted substrates. Although CRL4-based targeted protein degradation (TPD) has emerged as a promising strategy to modu...
Mol Cell
Molecular cell
2026 Apr 2
2,026
[ "Yamanaka S", "Nagaoka K", "Shoya Y", "Nishino K", "Mikura Y", "Tanaka K", "Konishi K", "Hasegawa Y", "Hijikata A", "Kosako H", "Sawasaki T" ]
Yamanaka S
Sawasaki T
[ "Humans", "HEK293 Cells", "Proteolysis", "DNA-Binding Proteins", "Cullin Proteins", "Ubiquitin-Protein Ligases", "Carrier Proteins", "Proteasome Endopeptidase Complex", "Ubiquitination", "Protein Binding", "Biotinylation", "Protein Interaction Mapping", "Protein Interaction Maps", "Peptide...
[ "Journal Article" ]
86
7
1397-1416.e11
null
1097-2765
eng
https://pubmed.ncbi.nlm.nih.gov/41932313/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41895285
10.1016/j.xcrm.2026.102719
Shinya Yamanaka
Dr. Shinya Yamanaka is recognized for the generation of induced pluripotent stem cells (iPSCs) from fibroblasts by a combination of multiple transcription factors, and he won the Nobel Prize in Physiology or Medicine in 2012 jointly with Sir John B. Gurdon for this discovery. Twenty years after the discovery, the Cell ...
Cell Rep Med
Cell reports. Medicine
2026 Apr 21
2,026
[ "Yamanaka S" ]
Yamanaka S
Yamanaka S
[ "Induced Pluripotent Stem Cells", "Humans", "History, 21st Century", "History, 20th Century", "Regenerative Medicine", "Animals", "Nobel Prize" ]
[ "Journal Article", "Historical Article" ]
7
4
102719
null
2666-3791
eng
https://pubmed.ncbi.nlm.nih.gov/41895285/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41852276
10.1021/acs.jmedchem.5c03771
Alkyne Two-Phase Strategy: Rapid Generation of TK-285-Derived PROTACs as BRD4 Degraders
This study introduces a divergent synthetic strategy in linkerology using preassembled linkers to generate structural diversity. The approach was validated by developing bromodomain-containing protein 4 (BRD4)-targeting proteolysis-targeting chimeras (PROTACs) based on an "alkyne two-phase strategy," employing the BRD4...
J Med Chem
Journal of medicinal chemistry
2026 Apr 9
2,026
[ "Yamakoshi H", "Watanabe R", "Segawa R", "Ishihara R", "Tachibana R", "Kudo G", "Nagasawa S", "Yamanaka S", "Ito A", "Takeda H", "Sawasaki T", "Yoshino R", "Hirokawa T", "Doi T", "Hirasawa N", "Iwabuchi Y" ]
Yamakoshi H
Iwabuchi Y
[ "Bromodomain Containing Proteins", "Proteolysis Targeting Chimera", "Cell Cycle Proteins", "Humans", "Transcription Factors", "Animals", "Alkynes", "Click Chemistry", "Structure-Activity Relationship", "Mice", "Triazoles", "Proteolysis" ]
[ "Journal Article" ]
69
7
8388-8416
pmc-id: PMC13071873;
0022-2623
eng
https://pubmed.ncbi.nlm.nih.gov/41852276/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41798681
10.1093/noajnl/vdag008
BRAF-mutant high-grade glioma with pleomorphic and pseudopapillary features (HPAP): A PLNTY mimic demonstrating tumor progression during longitudinal follow-up
High-grade glioma with pleomorphic and pseudopapillary features (HPAP) is a recently recognized glioma subtype defined by DNA methylation profiling. While it exhibits overlapping histological features with various CNS tumors, such as polymorphous low-grade neuroepithelial tumor of the young (PLNTY) and pleomorphic xant...
Neurooncol Adv
Neuro-oncology advances
2026 Jan-Dec
2,026
[ "Takayama Y", "Yaegashi M", "Satomi K", "Ishiyama T", "Shioda M", "Yazawa O", "Ye W", "Okagawa H", "Saito S", "Sasaoka K", "Okura M", "Koyama A", "Oshima A", "Sonoda M", "Natsumeda M", "Ichimura K", "Yamanaka S", "Fujii S", "Yamamoto T", "Tateishi K" ]
Takayama Y
Tateishi K
[]
[ "Journal Article" ]
8
1
vdag008
pmc-id: PMC12962799;
2632-2498
eng
https://pubmed.ncbi.nlm.nih.gov/41798681/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41795426
10.1016/j.stem.2026.02.003
Two decades of induced pluripotent stem cell research: From discovery to diverse applications
Twenty years have passed since the first demonstration of mouse induced pluripotent stem cells (iPSCs). What began as an unexpected observation in Kyoto quickly transformed stem cell biology and regenerative medicine worldwide. Over the past two decades, we have gained profound insights into the molecular mechanisms un...
Cell Stem Cell
Cell stem cell
2026 Mar 5
2,026
[ "Yamanaka S" ]
Yamanaka S
Yamanaka S
[ "Induced Pluripotent Stem Cells", "Animals", "Humans", "Cellular Reprogramming", "Regenerative Medicine", "Stem Cell Research" ]
[ "Journal Article", "Review" ]
33
3
372-381
null
1875-9777
eng
https://pubmed.ncbi.nlm.nih.gov/41795426/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41672211
10.1016/j.hrthm.2026.02.003
Linking the heart-eye-brain axis: Ocular and cerebral blood flow changes after catheter ablation in atrial fibrillation
null
Heart Rhythm
Heart rhythm
2026 Jul
2,026
[ "Yamamoto N", "Suzuki H", "Nakano M", "Yamanaka S", "Ito T", "Sato H", "Chiba T", "Nochioka K", "Takanami K", "Yasuda M", "Kunikata H", "Nakazawa T", "Yasuda S" ]
Yamamoto N
Yasuda S
[]
[ "Journal Article" ]
23
7
e1257-e1259
null
1547-5271
eng
https://pubmed.ncbi.nlm.nih.gov/41672211/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41668835
10.12890/2026_006191
B-Cell Lymphoma Following an Indolent Course Over 30 Years with Immunophenotypic Changes at Each Recurrence
Diffuse large B-cell lymphoma (DLBCL) comprises biologically distinct subtypes, but whether cell-of-origin-related features remain stable over long disease courses is unclear. A woman was initially diagnosed with CD20-positive B-cell lymphoma in 1988 and treated with cyclophosphamide, vincristine and prednisolone (COP)...
Eur J Case Rep Intern Med
European journal of case reports in internal medicine
2026
2,026
[ "Yamanaka S", "Hasebe S", "Fujii T", "Gondo T", "Kitazawa R", "Kitazawa S", "Yakushijin Y" ]
Yamanaka S
Yakushijin Y
[]
[ "Journal Article" ]
13
2
006191
pmc-id: PMC12885577;
2284-2594
eng
https://pubmed.ncbi.nlm.nih.gov/41668835/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41633958
10.1111/aas.70197
Intraoperative Hypotension and Vasoactive Treatment: An International Survey of Anaesthesiologists
Intraoperative hypotension is a common occurrence in patients undergoing anaesthesia, although there is no standardised definition of hypotension. International consensus statements provide some guidelines for the management of intraoperative hypotension, but general clinical practice is unknown. We aimed to survey ana...
Acta Anaesthesiol Scand
Acta anaesthesiologica Scandinavica
2026 Mar
2,026
[ "Bækgaard ES", "Vester-Andersen M", "Crone V", "Møller MH", "Yamanaka S", "Palmarsdottir R", "Uusalo P", "Haidl F", "Rådestad M", "van der Sloot K", "Corona A", "Johnström A", "Nørskov AK", "Karlsen APH", "Faustad BI", "Sørensen CB", "Spies F", "Krogh HB", "El-Hallak H", "Mistr...
Bækgaard ES
Krag M
[ "Humans", "Intraoperative Complications", "Hypotension", "Vasoconstrictor Agents", "Anesthesiologists", "Adult", "Female", "Surveys and Questionnaires", "Male", "Europe", "Middle Aged" ]
[ "Journal Article" ]
70
3
e70197
pmc-id: PMC12895218;
0001-5172
eng
https://pubmed.ncbi.nlm.nih.gov/41633958/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41613647
10.21769/BioProtoc.5565
Protocol for In Utero Fetal-to-Fetal Kidney Transplantation in Rats
Congenital renal disorders, such as the Potter sequence, result from renal dysgenesis. To explore a prenatal therapeutic approach for fetuses with kidney insufficiency, we established an in utero transplantation protocol using donor fetal kidneys. Although numerous rodent studies have reported cellular injections into ...
Bio Protoc
Bio-protocol
2026 Jan 20
2,026
[ "Morimoto K", "Yamanaka S", "Yokoo T" ]
Morimoto K
Yokoo T
[]
[ "Journal Article" ]
16
2
e5565
pmc-id: PMC12848318;
2331-8325
eng
https://pubmed.ncbi.nlm.nih.gov/41613647/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41569678
10.3171/CASE25705
Complete remission of intracranial lymphocytic vasculitis following resection: illustrative case
Lymphocytic vasculitis (LV), a histopathological subtype of primary angiitis of the CNS, is a rare inflammatory disorder that often presents as mass-like lesions mimicking malignant brain tumors. Owing to its rarity, the characteristics of LV remain poorly understood. The authors report the case of a young woman who pr...
J Neurosurg Case Lessons
Journal of neurosurgery. Case lessons
2026 Jan 19
2,026
[ "Yazawa O", "Tateishi K", "Ikegaya N", "Kawai T", "Harada J", "Kato A", "Morihara K", "Yamanaka S", "Utsunomiya D", "Fujii S", "Tanaka F", "Yamamoto T" ]
Yazawa O
Yamamoto T
[]
[ "Journal Article" ]
11
3
null
pmc-id: PMC12814156;
2694-1902
eng
https://pubmed.ncbi.nlm.nih.gov/41569678/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41557950
10.1021/acs.jpclett.5c03517
Real-Time Sub-Molecular Visualization of DNA Folding Using a Hybrid High-Speed AFM and Optical Tweezers System
Optical tweezers have contributed to elucidating the folding mechanisms associated with biomolecules. By combining them with fluorescence imaging techniques, imaging can also be performed while measuring or applying forces that couple to biochemical reactions; however, they cannot capture structural information beyond ...
J Phys Chem Lett
The journal of physical chemistry letters
2026 Jan 29
2,026
[ "Umeda K", "Yamanaka S", "Imamura M", "Nagae F", "Fukuda S", "Watanabe H", "Uchihashi T", "Takada S", "Ando T" ]
Umeda K
Ando T
[ "Optical Tweezers", "Microscopy, Atomic Force", "Nucleic Acid Conformation", "DNA", "Molecular Dynamics Simulation", "DNA, Single-Stranded" ]
[ "Journal Article" ]
17
4
1182-1191
null
1948-7185
eng
https://pubmed.ncbi.nlm.nih.gov/41557950/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41486379
10.1038/s41440-025-02449-9
Prognostic impact of systolic blood pressure and antithrombotic strategy in patients with atrial fibrillation and stable coronary artery disease: a post-hoc analysis of the AFIRE trial
The AFIRE (Atrial Fibrillation and Ischemic Events with Rivaroxaban in Patients with Stable Coronary Artery Disease) trial demonstrated that rivaroxaban monotherapy was non-inferior in efficacy and superior in safety compared to rivaroxaban plus single antiplatelet therapy in patients with atrial fibrillation (AF) and ...
Hypertens Res
Hypertension research : official journal of the Japanese Society of Hypertension
2026 Apr
2,026
[ "Yamanaka S", "Noda T", "Nochioka K", "Higuma T", "Kaikita K", "Akao M", "Ako J", "Matoba T", "Nakamura M", "Miyauchi K", "Hagiwara N", "Kimura K", "Matsui K", "Ogawa H", "Yasuda S", "AFIRE Investigators" ]
Yamanaka S
Yasuda S
[ "Humans", "Atrial Fibrillation", "Male", "Female", "Coronary Artery Disease", "Aged", "Blood Pressure", "Rivaroxaban", "Middle Aged", "Prognosis", "Fibrinolytic Agents", "Factor Xa Inhibitors", "Platelet Aggregation Inhibitors", "Treatment Outcome" ]
[ "Journal Article", "Randomized Controlled Trial", "Multicenter Study", "Research Support, Non-U.S. Gov't" ]
49
4
1139-1149
pmc-id: PMC13050638;
0916-9636
eng
https://pubmed.ncbi.nlm.nih.gov/41486379/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41380574
10.1016/j.anl.2025.12.004
C-C chemokine receptor 4 is a candidate for regulatory T-cell-depletion immunotherapy in differentiated thyroid cancer
Immunotherapy using immune checkpoint inhibitors is a standard treatment option for many types of malignancies but is not effective for differentiated thyroid cancer (DTC). Regulatory T-cells (Tregs), which are one of the main suppressive factors in the tumor microenvironment, are another important target for immunothe...
Auris Nasus Larynx
Auris, nasus, larynx
2026 Feb
2,026
[ "Sano R", "Nakamura H", "Suzuki S", "Inukai D", "Okamoto H", "Yamanaka S", "Takahara T", "Satou A", "Fujimoto Y", "Tsuzuki T", "Ueda R", "Ogawa T" ]
Sano R
Ogawa T
[ "Humans", "Male", "Female", "T-Lymphocytes, Regulatory", "Thyroid Neoplasms", "Middle Aged", "Adult", "Aged", "Receptors, CCR4", "Aged, 80 and over", "Immunotherapy", "Lymphocytes, Tumor-Infiltrating", "Forkhead Transcription Factors", "Tumor Microenvironment", "Leukocyte Common Antigens...
[ "Journal Article" ]
53
1
27-34
null
0385-8146
eng
https://pubmed.ncbi.nlm.nih.gov/41380574/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41349636
10.1016/j.jtct.2025.11.040
Comparison of Myeloablative Busulfan/Cyclophosphamide and Busulfan/Fludarabine for Allogeneic Hematopoietic Stem Cell Transplantation: A Systematic Review and Meta-Analysis
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a curative treatment for many hematological malignancies. The busulfan plus cyclophosphamide (Bu/Cy) combination is a standard myeloablative conditioning (MAC) regimen, but it is associated with significant regimen-related toxicity and non-relapse mortal...
Transplant Cell Ther
Transplantation and cellular therapy
2026 Apr
2,026
[ "Shimada T", "Masaki T", "Koyamada R", "Ishikawa K", "Yamanaka S", "Sato K", "Ota E", "Yamashita T", "Mori S" ]
Shimada T
Mori S
[ "Humans", "Busulfan", "Hematopoietic Stem Cell Transplantation", "Vidarabine", "Cyclophosphamide", "Transplantation Conditioning", "Transplantation, Homologous", "Myeloablative Agonists", "Hematologic Neoplasms" ]
[ "Journal Article", "Systematic Review", "Meta-Analysis", "Comparative Study" ]
32
4
505.e1-505.e11
null
2666-6367
eng
https://pubmed.ncbi.nlm.nih.gov/41349636/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41326368
10.1038/s41419-025-08243-2
Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
Ovarian clear cell carcinoma is characterized by HNF-1ß overexpression and is known to be resistant to chemotherapy. An inhibitor screening that specifically targets HNF-1ß led us to identify Actinonin as a candidate for cancer treatment. Actinonin, which is known to inhibit aminopeptidase M, has also been recognized f...
Cell Death Dis
Cell death & disease
2025 Dec 1
2,025
[ "Kawahara N", "Kuniyasu H", "Mori S", "Kishi S", "Sugimoto S", "Maehana T", "Yamanaka S", "Kawaguchi R", "Kimura F" ]
Kawahara N
Kimura F
[ "Reactive Oxygen Species", "Humans", "Glycolysis", "Cell Line, Tumor", "Mitochondria", "Female", "Animals", "Adenocarcinoma, Clear Cell", "Glycogen Synthase Kinase 3 beta", "Organelle Biogenesis", "Cell Death", "Mice", "Carcinoma, Renal Cell", "Mice, Nude", "Ovarian Neoplasms", "Kidney...
[ "Journal Article" ]
16
1
879
pmc-id: PMC12669797;
2041-4889
eng
https://pubmed.ncbi.nlm.nih.gov/41326368/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41293569
10.1016/j.gore.2025.101979
Malignant peritoneal mesothelioma as a secondary cancer following radiotherapy for cervical cancer: a case report and literature review
Malignant peritoneal mesothelioma (MPM) as a secondary malignancy following radiotherapy is extremely rare. We report a case of MPM that developed as a secondary malignancy after initial concurrent chemoradiotherapy (CCRT) for cervical cancer.Case Presentation.A 52-year-old woman with no history of asbestos exposure un...
Gynecol Oncol Rep
Gynecologic oncology reports
2025 Dec
2,025
[ "Otsubo M", "Aichi M", "Kawashima Y", "Yamanaka S", "Imai Y", "Miyagi E", "Mizushima T" ]
Otsubo M
Mizushima T
[]
[ "Case Reports", "Journal Article" ]
62
null
101979
pmc-id: PMC12641188;
2352-5789
eng
https://pubmed.ncbi.nlm.nih.gov/41293569/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41284624
10.1371/journal.pone.0337375
Correction: Generation of Human Melanocytes from Induced Pluripotent Stem Cells
[This corrects the article DOI: 10.1371/journal.pone.0016182.].
PLoS One
PloS one
2025
2,025
[ "Ohta S", "Imaizumi Y", "Okada Y", "Akamatsu W", "Kuwahara R", "Ohyama M", "Amagai M", "Matsuzaki Y", "Yamanaka S", "Okano H", "Kawakami Y" ]
Ohta S
Kawakami Y
[]
[ "Published Erratum" ]
20
11
e0337375
pmc-id: PMC12643287;
1932-6203
eng
https://pubmed.ncbi.nlm.nih.gov/41284624/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41254879
10.1111/ped.70275
A true brachial artery aneurysm in a 1-year-old child: A case report
null
Pediatr Int
Pediatrics international : official journal of the Japan Pediatric Society
2025 Jan-Dec
2,025
[ "Kawada A", "Ichikawa Y", "Goda M", "Yamanaka S" ]
Kawada A
Yamanaka S
[]
[ "Journal Article" ]
67
1
e70275
null
1328-8067
eng
https://pubmed.ncbi.nlm.nih.gov/41254879/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41233468
10.1038/s41598-025-23608-5
Treatment of initially unresectable local advanced pancreatic adenocarcinoma with FOLFIRINOX: A prospective study YCOG1403 (C-FLAP study)
This study aimed to provide evidence regarding the treatment of patients with unresectable pancreatic cancer. We conducted a prospective single-arm phase II trial using the FOLFIRINOX regimen. After completing 4-8 cycles, patients underwent surgical resection when feasible. The primary endpoint was R0 resection rate. F...
Sci Rep
Scientific reports
2025 Nov 13
2,025
[ "Kobayashi N", "Yabushita Y", "Mori R", "Takahashi T", "Miyake K", "Sawada Y", "Homma Y", "Matsuyama R", "Okubo N", "Katsuta E", "Kubota K", "Yamanaka S", "Ichikawa Y", "Endo I" ]
Kobayashi N
Endo I
[ "Humans", "Pancreatic Neoplasms", "Male", "Antineoplastic Combined Chemotherapy Protocols", "Female", "Middle Aged", "Leucovorin", "Fluorouracil", "Oxaliplatin", "Irinotecan", "Aged", "Prospective Studies", "Adenocarcinoma", "Adult", "Treatment Outcome" ]
[ "Journal Article", "Clinical Trial, Phase II" ]
15
1
39884
pmc-id: PMC12615679;
2045-2322
eng
https://pubmed.ncbi.nlm.nih.gov/41233468/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41169952
10.1016/j.gore.2025.101937
Embryonal rhabdomyosarcoma of the uterine corpus in a postmenopausal Woman: A case report and literature Review
Rhabdomyosarcoma (RMS) is a rare malignant mesenchymal tumor, primarily affecting children. Occurrence in the uterine corpus of adults, particularly postmenopausal women, is extremely uncommon. A 60-year-old postmenopausal nulligravid woman presented with abnormal genital bleeding for three months. Pelvic examination r...
Gynecol Oncol Rep
Gynecologic oncology reports
2025 Oct
2,025
[ "Omuro M", "Imai Y", "Ogawara Y", "Mizushima T", "Muraoka E", "Yamanaka S", "Fujii S", "Miyagi E" ]
Omuro M
Miyagi E
[]
[ "Journal Article", "Review" ]
61
null
101937
pmc-id: PMC12570091;
2352-5789
eng
https://pubmed.ncbi.nlm.nih.gov/41169952/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41107244
10.1038/s41467-025-64277-2
Observation of a guest-free Si(46) clathrate-I framework from Ba(8-x)Si(46) upon in situ vacuum heating
Silicon and its composites are key materials owing to their extensive use in the semiconductor industry. While the diamond-structured form dominates, other allotropes with superior properties at ambient conditions remain of interest. Toward this, Si46 clathrate type-I crystals containing alkali/alkaline-earth metals ha...
Nat Commun
Nature communications
2025 Oct 17
2,025
[ "Zhou Y", "Zhang Q", "Mayoral A", "Spackman PR", "Matsumoto T", "Li J", "Gale JD", "Anderson MW", "Fukuoka H", "Yamanaka S", "Terasaki O" ]
Zhou Y
Terasaki O
[]
[ "Journal Article" ]
16
1
9238
pmc-id: PMC12534387;
2041-1723
eng
https://pubmed.ncbi.nlm.nih.gov/41107244/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41069488
10.1016/j.ijcha.2025.101812
New-onset anemia and its association with ventricular arrhythmias and sudden cardiac death in patients with heart failure with preserved ejection fraction
Anemia is a common comorbidity associated with adverse outcomes in patients with heart failure with preserved ejection fraction (HFpEF). However, the clinical relevance of new-onset anemia to sudden cardiac death (SCD) in patients with HFpEF remains unclear. This study investigated the association between new-onset ane...
Int J Cardiol Heart Vasc
International journal of cardiology. Heart & vasculature
2025 Dec
2,025
[ "Ito T", "Nochioka K", "Noda T", "Shiroto T", "Yamanaka S", "Yamamoto N", "Sato H", "Chiba T", "Nakano M", "Inoue T", "Susukita K", "Takahama H", "Takahashi J", "Miyata S", "Shimokawa H", "Yasuda S" ]
Ito T
Yasuda S
[]
[ "Journal Article" ]
61
null
101812
pmc-id: PMC12506443;
2352-9067
eng
https://pubmed.ncbi.nlm.nih.gov/41069488/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41067467
10.1016/j.hrthm.2025.09.040
Incidence and clinical implications of microbubble formation during pulsed field ablation
null
Heart Rhythm
Heart rhythm
2026 Feb
2,026
[ "Yano S", "Takatsuki S", "Kotajima T", "Sasajima K", "Yamanaka S", "Himeno Y", "Yamashita S", "Yamaoka K", "Katsumata Y", "Nishiyama T", "Kimura T", "Ieda M" ]
Yano S
Ieda M
[]
[ "Journal Article" ]
23
2
e276-e277
null
1547-5271
eng
https://pubmed.ncbi.nlm.nih.gov/41067467/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
41064542
10.1159/000547424
Successful Treatment of Refractory Immune-Mediated Colitis and Duodenitis with Tofacitinib
Immune-mediated colitis (IMC) is a common and potentially severe immune-related adverse event of immune checkpoint inhibitors (ICIs). Although corticosteroids and biologics are standard treatments, some cases remain refractory. Tofacitinib, a Janus kinase inhibitor, has shown promise in refractory IMC; however, evidenc...
Case Rep Gastroenterol
Case reports in gastroenterology
2025 Jan-Dec
2,025
[ "Misawa N", "Higurashi T", "Inoue K", "Suzuki H", "Tamura S", "Yoneda M", "Hori C", "Yamanaka S", "Nakajima A" ]
Misawa N
Nakajima A
[]
[ "Case Reports", "Journal Article" ]
19
1
621-629
pmc-id: PMC12503550;
1662-0631
eng
https://pubmed.ncbi.nlm.nih.gov/41064542/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40909319
10.1002/iju5.70072
A Case of Renal Metastasis of Urothelial Carcinoma With Trophoblastic Differentiation Successfully Treated With Robot-Assisted Partial Nephrectomy
There are no previous reports of solitary renal metastases from urothelial carcinoma with trophoblastic differentiation, a rare bladder cancer subtype that is pathologically hCGβ positive. A 77-year-old male with urothelial carcinoma with trophoblastic differentiation underwent robot-assisted radical cystectomy followi...
IJU Case Rep
IJU case reports
2025 Sep
2,025
[ "Honda S", "Uemura K", "Ito H", "Muraoka E", "Tatenuma T", "Ito Y", "Muraoka K", "Hasumi H", "Kawano N", "Yamanaka S", "Makiyama K" ]
Honda S
Makiyama K
[]
[ "Journal Article" ]
8
5
480-484
pmc-id: PMC12408180;
2577-171X
eng
https://pubmed.ncbi.nlm.nih.gov/40909319/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40885760
10.1038/s42003-025-08761-x
In-cell proximity target validation methods for heterobifunctional molecules with CRBN- or VHL-binder using AirID
Heterobifunctional molecules, such as proteolysis-targeting and autophagy-targeting chimera, represent new drug concepts. They are composed of two protein binders that can induce proximity interactions between two proteins and protein catalysis. Currently, cereblon (CRBN)- and von Hippel-Lindau (VHL)-binders with thali...
Commun Biol
Communications biology
2025 Aug 30
2,025
[ "Yamada K", "Yamanaka S", "Yamakoshi H", "Kohyama A", "Iwabuchi Y", "Kosako H", "Sawasaki T" ]
Yamada K
Sawasaki T
[ "Von Hippel-Lindau Tumor Suppressor Protein", "Humans", "Ubiquitin-Protein Ligases", "Adaptor Proteins, Signal Transducing", "Protein Binding", "HEK293 Cells", "Biotinylation", "Thalidomide" ]
[ "Journal Article" ]
8
1
1323
pmc-id: PMC12398604;
2399-3642
eng
https://pubmed.ncbi.nlm.nih.gov/40885760/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40840899
null
[A Case of Liver and Axillary Lymph Node Metastasis 32 Years after Breast Cancer Surgery]
unknown, but she remained symptom-free for 32 years. However, a CT scan performed for an unrelated condition revealed 2 liver tumors with calcifications located in S8(10 cm)and S6(3 cm). Given her medical history, liver metastasis from breast cancer was suspected. Core needle biopsies of the liver tumor and a left axil...
Gan To Kagaku Ryoho
Gan to kagaku ryoho. Cancer & chemotherapy
2025 Jul
2,025
[ "Aikawa A", "Oshi M", "Kawashima K", "Sasamoto M", "Fujiwara Y", "Adachi S", "Narui K", "Takase H", "Yamanaka S", "Fujii S", "Yamada A", "Endo I" ]
Aikawa A
Endo I
[ "Humans", "Breast Neoplasms", "Female", "Liver Neoplasms", "Lymphatic Metastasis", "Axilla", "Antineoplastic Combined Chemotherapy Protocols", "Time Factors", "Middle Aged" ]
[ "Journal Article", "Case Reports", "English Abstract" ]
52
7
529-531
null
0385-0684
jpn
https://pubmed.ncbi.nlm.nih.gov/40840899/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40672138
10.47371/mycosci.2024.11.002
Root-associated ectomycorrhizal fungal communities in and around aggregated retention patches left in logged areas of Abies sachalinensis planted forests
An aggregated retention system retains several groups of trees within cutblocks to maintain public functions such as biodiversity conservation. We examined ectomycorrhizal (EcM) fungal communities associated with regenerating Abies sachalinensis seedlings and their surrounding trees at different locations; inside and a...
Mycoscience
Mycoscience
2025
2,025
[ "Obase K", "Yamanaka S", "Ozaki K" ]
Obase K
Ozaki K
[]
[ "Journal Article" ]
66
1
116-119
pmc-id: PMC12260387;
1340-3540
eng
https://pubmed.ncbi.nlm.nih.gov/40672138/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40624739
10.1097/TP.0000000000005480
Surgical Integration of Metanephros-bladder Composite Tissue Transplants in Adult Pigs
null
Transplantation
Transplantation
2025 Oct 1
2,025
[ "Kinoshita Y", "Yamanaka S", "Iwai S", "Kume H", "Yokoo T", "Kobayashi E" ]
Kinoshita Y
Kobayashi E
[]
[ "Journal Article" ]
109
10
e621-e622
null
0041-1337
eng
https://pubmed.ncbi.nlm.nih.gov/40624739/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40578765
10.1016/j.xfss.2025.06.002
Transcriptome landscapes induced by estetrol, estradiol, and ethinylestradiol in primary human endometriotic stromal cells
To compare the transcriptomes induced by estrogens used in combined oral contraceptives (COCs): estetrol (E4), ethinylestradiol (EE), and estradiol (E2) in human primary endometriotic stromal cells. Endometriotic stromal cells were cultured in the presence of E4 (10-6 mol/L), E2 (10-8 mol/L) or EE (10-9 mol/L) for 24 h...
F S Sci
F&S science
2025 Nov
2,025
[ "Nakajima M", "Izumi G", "Koga K", "Silvana V", "Elsherbini M", "Okumura A", "Wada M", "Yamanaka S", "Hirota Y", "Osuga Y" ]
Nakajima M
Osuga Y
[ "Humans", "Ethinyl Estradiol", "Female", "Stromal Cells", "Estradiol", "Transcriptome", "Estetrol", "Endometriosis", "Endometrium", "Cells, Cultured", "Adult" ]
[ "Journal Article" ]
6
4
387-396
null
2666-335X
eng
https://pubmed.ncbi.nlm.nih.gov/40578765/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40425850
10.1038/s41390-025-04123-9
A novel mouse model for investigating the long-term impact of reduced nephron numbers on renal function and salt sensitivity
Nephrogenesis occurs during the fetal period; because nephrogenesis does not occur after birth, preterm infants have low nephron numbers. However, whether a reduction in the number of pure nephrons alone affects renal function in conventional animal models remains unclear. Therefore, we aimed to examine whether differe...
Pediatr Res
Pediatric research
2025 Nov
2,025
[ "Inage Y", "Matsumoto K", "Haruhara K", "Hirano D", "Matsui K", "Kinoshita Y", "Morimoto K", "Koda N", "Yamamoto S", "Fujimoto T", "Fukunaga S", "Yamanaka S", "Oishi K", "Kobayashi E", "Yokoo T" ]
Inage Y
Yokoo T
[ "Animals", "Nephrons", "Mice, Transgenic", "Mice", "Sodium Chloride, Dietary", "Kidney", "Female", "Disease Models, Animal", "Diphtheria Toxin", "Male" ]
[ "Journal Article" ]
98
5
1941-1949
null
0031-3998
eng
https://pubmed.ncbi.nlm.nih.gov/40425850/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40355784
10.1007/s10147-025-02787-1
Impact of body mass index on D-dimer diagnostic utility for deep vein thrombosis in patients with cancer: a single-center retrospective analysis
Deep vein thrombosis (DVT) is a common complication in cancer patients associated with significant morbidity and mortality. D-dimer is a widely used screening tool for suspected DVT; however, its accuracy may be influenced by body mass index (BMI). We evaluated whether BMI modified the diagnostic utility of D-dimer lev...
Int J Clin Oncol
International journal of clinical oncology
2025 Aug
2,025
[ "Masuda Y", "Konishi T", "Yakushijin Y", "Yamanaka S", "Hasebe S", "Yamanouchi J", "Takenaka K" ]
Masuda Y
Takenaka K
[ "Humans", "Fibrin Fibrinogen Degradation Products", "Venous Thrombosis", "Male", "Female", "Retrospective Studies", "Neoplasms", "Body Mass Index", "Middle Aged", "Aged", "Adult", "Aged, 80 and over" ]
[ "Journal Article" ]
30
8
1545-1552
null
1341-9625
eng
https://pubmed.ncbi.nlm.nih.gov/40355784/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40255925
10.2176/jns-nmc.2024-0234
Intrathecal Baclofen Therapy Improves Refractory Status Dystonicus in Neuro-hepatic Wilson's Disease: A Case Report
Wilson's disease is an autosomal recessive disorder of copper metabolism. A current unresolved issue is the worsening of neurological symptoms during the initial treatment phase, particularly with chelation therapy. This phenomenon, termed "early neurological worsening," is attributed to the rapid mobilization and redi...
NMC Case Rep J
NMC case report journal
2025
2,025
[ "Yamanaka S", "Hanada T", "Higashi T", "Matsunaga M", "Yonee C", "Maruyama S", "Hanaya R" ]
Yamanaka S
Hanaya R
[]
[ "Case Reports", "Journal Article" ]
12
null
91-95
pmc-id: PMC12009644;
2188-4226
eng
https://pubmed.ncbi.nlm.nih.gov/40255925/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40230291
10.1002/ejhf.3654
Clinical and plasma proteomic characterization of heart failure with supranormal left ventricular ejection fraction: An emerging entity of heart failure
The clinical guidelines categorize heart failure (HF) based on left ventricular ejection fraction (LVEF). However, the current LVEF cutoffs, 40% and 50%, may not fully address the underlying characteristics and cardiovascular risk of HF, particularly for HF with higher LVEF. This study aimed to characterize HF with sup...
Eur J Heart Fail
European journal of heart failure
2025 Aug
2,025
[ "Sakata Y", "Nochioka K", "Yasuda S", "Ishida K", "Shiroto T", "Takahashi J", "Kasahara S", "Abe R", "Yamanaka S", "Fujihashi T", "Hayashi H", "Kato S", "Horii K", "Teramoto K", "Tomita T", "Miyata S", "Sugimura K", "Waga I", "Nagasaki M", "Shimokawa H" ]
Sakata Y
Shimokawa H
[ "Humans", "Heart Failure", "Stroke Volume", "Female", "Male", "Aged", "Proteomics", "Ventricular Function, Left", "Middle Aged", "Prognosis" ]
[ "Journal Article" ]
27
8
1570-1583
pmc-id: PMC12482836;
1388-9842
eng
https://pubmed.ncbi.nlm.nih.gov/40230291/
3a2re71Ek4OjT7tM5
2s5WUk1iDLbp7ghNy
40670800
10.1038/s41586-025-09355-7
Author Correction: BNT162b2 vaccine induces neutralizing antibodies and poly-specific T cells in humans
null
Nature
Nature
2025 Jul
2,025
[ "Şahin U", "Muik A", "Vogler I", "Derhovanessian E", "Kranz LM", "Vormehr M", "Quandt J", "Bidmon N", "Ulges A", "Baum A", "Pascal KE", "Maurus D", "Brachtendorf S", "Lörks V", "Sikorski J", "Koch P", "Hilker R", "Becker D", "Eller AK", "Grützner J", "Tonigold M", "Boesler ...
Şahin U
Türeci Ö
[]
[ "Published Erratum" ]
643
8074
E27
null
0028-0836
eng
https://pubmed.ncbi.nlm.nih.gov/40670800/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
37876532
10.1016/j.omtn.2023.102045
Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice
The response to mRNA vaccines needs to be sufficient for immune cell activation and recruitment, but moderate enough to ensure efficacious antigen expression. The choice of the cap structure and use of N1-methylpseudouridine (m1Ψ) instead of uridine, which have been shown to reduce RNA sensing by the cellular innate im...
Mol Ther Nucleic Acids
Molecular therapy. Nucleic acids
2023 Dec 12
2,023
[ "Wang Z", "Jacobus EJ", "Stirling DC", "Krumm S", "Flight KE", "Cunliffe RF", "Mottl J", "Singh C", "Mosscrop LG", "Santiago LA", "Vogel AB", "Kariko K", "Sahin U", "Erbar S", "Tregoning JS" ]
Wang Z
Tregoning JS
[]
[ "Journal Article" ]
34
null
102045
pmc-id: PMC10591005;
2162-2531
eng
https://pubmed.ncbi.nlm.nih.gov/37876532/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
37371829
10.3390/biomedicines11061735
ASL mRNA-LNP Therapeutic for the Treatment of Argininosuccinic Aciduria Enables Survival Benefit in a Mouse Model
Argininosuccinic aciduria (ASA) is a metabolic disorder caused by a deficiency in argininosuccinate lyase (ASL), which cleaves argininosuccinic acid to arginine and fumarate in the urea cycle. ASL deficiency (ASLD) leads to hepatocyte dysfunction, hyperammonemia, encephalopathy, and respiratory alkalosis. Here we descr...
Biomedicines
Biomedicines
2023 Jun 16
2,023
[ "Daly O", "Mahiny AJ", "Majeski S", "McClintock K", "Reichert J", "Boros G", "Szabó GT", "Reinholz J", "Schreiner P", "Reid S", "Lam K", "Lepper M", "Adler M", "Meffen T", "Heyes J", "Karikó K", "Lutwyche P", "Vlatkovic I" ]
Daly O
Vlatkovic I
[]
[ "Journal Article" ]
11
6
null
pmc-id: PMC10296609;
2227-9059
eng
https://pubmed.ncbi.nlm.nih.gov/37371829/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
37020354
10.1111/nyas.14975
Progress in vaccine development for infectious diseases-a Keystone Symposia report
The COVID-19 pandemic has taught us many things, among the most important of which is that vaccines are one of the cornerstones of public health that help make modern longevity possible. While several different vaccines have been successful at stemming the morbidity and mortality associated with various infectious dise...
Ann N Y Acad Sci
Annals of the New York Academy of Sciences
2023 Jun
2,023
[ "Cable J", "Graham BS", "Koup RA", "Seder RA", "Karikó K", "Pardi N", "Barouch DH", "Sharma B", "Rauch S", "Nachbagauer R", "Forsell MNE", "Schotsaert M", "Ellebedy AH", "Loré K", "Irvine DJ", "Pilkington E", "Tahtinen S", "Thompson EA", "Feraoun Y", "King NP", "Saunders K", ...
Cable J
Bekker LG
[ "Humans", "Pandemics", "COVID-19", "Vaccines", "Communicable Diseases", "Vaccination", "Vaccine Development" ]
[ "Journal Article", "Research Support, Non-U.S. Gov't", "Research Support, N.I.H., Extramural" ]
1524
1
65-86
null
0077-8923
eng
https://pubmed.ncbi.nlm.nih.gov/37020354/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
36867683
10.1126/scitranslmed.abn3464
Single immunizations of self-amplifying or non-replicating mRNA-LNP vaccines control HPV-associated tumors in mice
As mRNA vaccines have proved to be very successful in battling the coronavirus disease 2019 (COVID-19) pandemic, this new modality has attracted widespread interest for the development of potent vaccines against other infectious diseases and cancer. Cervical cancer caused by persistent human papillomavirus (HPV) infect...
Sci Transl Med
Science translational medicine
2023 Mar 8
2,023
[ "Ramos da Silva J", "Bitencourt Rodrigues K", "Formoso Pelegrin G", "Silva Sales N", "Muramatsu H", "de Oliveira Silva M", "Porchia BFMM", "Moreno ACR", "Aps LRMM", "Venceslau-Carvalho AA", "Tombácz I", "Fotoran WL", "Karikó K", "Lin PJC", "Tam YK", "de Oliveira Diniz M", "Pardi N", ...
Ramos da Silva J
de Souza Ferreira LC
[ "Animals", "Female", "Mice", "Cancer Vaccines", "CD8-Positive T-Lymphocytes", "Disease Models, Animal", "Immunization", "Mice, Inbred C57BL", "Neoplasms", "Papillomavirus E7 Proteins", "Papillomavirus Infections", "Papillomavirus Vaccines", "Recombinant Proteins", "RNA, Messenger", "Vacc...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
15
686
eabn3464
null
1946-6234
eng
https://pubmed.ncbi.nlm.nih.gov/36867683/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
36167467
10.1136/jitc-2021-004450
Development of GPC2-directed chimeric antigen receptors using mRNA for pediatric brain tumors
Pediatric brain tumors are the leading cause of cancer death in children with an urgent need for innovative therapies. Glypican 2 (GPC2) is a cell surface oncoprotein expressed in neuroblastoma for which targeted immunotherapies have been developed. This work aimed to characterize GPC2 expression in pediatric brain tum...
J Immunother Cancer
Journal for immunotherapy of cancer
2022 Sep
2,022
[ "Foster JB", "Griffin C", "Rokita JL", "Stern A", "Brimley C", "Rathi K", "Lane MV", "Buongervino SN", "Smith T", "Madsen PJ", "Martinez D", "Delaidelli A", "Sorensen PH", "Wechsler-Reya RJ", "Karikó K", "Storm PB", "Barrett DM", "Resnick AC", "Maris JM", "Bosse KR" ]
Foster JB
Bosse KR
[ "Brain Neoplasms", "Cell Line, Tumor", "Cerebellar Neoplasms", "Child", "Glioma", "Glypicans", "Humans", "Medulloblastoma", "Neuroblastoma", "Oncogene Proteins", "RNA, Messenger", "Receptors, Chimeric Antigen", "Single-Chain Antibodies", "Xenograft Model Antitumor Assays" ]
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
10
9
null
pmc-id: PMC9516314;
2051-1426
eng
https://pubmed.ncbi.nlm.nih.gov/36167467/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
35704995
10.1016/j.immuni.2022.05.007
Lipid nanoparticles enhance the efficacy of mRNA and protein subunit vaccines by inducing robust T follicular helper cell and humoral responses
null
Immunity
Immunity
2022 Jun 14
2,022
[ "Alameh MG", "Tombácz I", "Bettini E", "Lederer K", "Sittplangkoon C", "Wilmore JR", "Gaudette BT", "Soliman OY", "Pine M", "Hicks P", "Manzoni TB", "Knox JJ", "Johnson JL", "Laczkó D", "Muramatsu H", "Davis B", "Meng W", "Rosenfeld AM", "Strohmeier S", "Lin PJC", "Mui BL", ...
Alameh MG
Pardi N
[]
[ "Published Erratum" ]
55
6
1136-1138
pmc-id: PMC9195404;
1074-7613
eng
https://pubmed.ncbi.nlm.nih.gov/35704995/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
35536311
10.1093/nar/gkac347
A systematic dissection of determinants and consequences of snoRNA-guided pseudouridylation of human mRNA
RNA can be extensively modified post-transcriptionally with >170 covalent modifications, expanding its functional and structural repertoire. Pseudouridine (Ψ), the most abundant modified nucleoside in rRNA and tRNA, has recently been found within mRNA molecules. It remains unclear whether pseudouridylation of mRNA can ...
Nucleic Acids Res
Nucleic acids research
2022 May 20
2,022
[ "Nir R", "Hoernes TP", "Muramatsu H", "Faserl K", "Karikó K", "Erlacher MD", "Sas-Chen A", "Schwartz S" ]
Nir R
Schwartz S
[ "Humans", "Protein Biosynthesis", "Pseudouridine", "RNA Processing, Post-Transcriptional", "RNA, Messenger", "RNA, Ribosomal", "RNA, Small Nucleolar" ]
[ "Journal Article" ]
50
9
4900-4916
pmc-id: PMC9122591;
0305-1048
eng
https://pubmed.ncbi.nlm.nih.gov/35536311/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
35342149
10.2302/kjm.71-001-ABST
Developing mRNA for Therapy
Messenger RNA was discovered in 1961 and it took 60 years until the first mRNA became FDA-approved product in the form of COVID-19 mRNA vaccine. During those years a lot of progress has been made by hundreds of scientists. It was 1978 when the first-time isolated mRNA delivered into mammalian cells produced the encoded...
Keio J Med
The Keio journal of medicine
2022
2,022
[ "Karikó K" ]
Karikó K
Karikó K
[ "Animals", "COVID-19", "COVID-19 Vaccines", "Humans", "Liposomes", "Mammals", "Nanoparticles", "RNA, Messenger", "Vaccines, Synthetic", "mRNA Vaccines" ]
[ "Journal Article" ]
71
1
31
null
0022-9717
eng
https://pubmed.ncbi.nlm.nih.gov/35342149/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
35214060
10.3390/pharmaceutics14020328
Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA
The presence of the cap structure on the 5'-end of in vitro-transcribed (IVT) mRNA determines its translation and stability, underpinning its use in therapeutics. Both enzymatic and co-transcriptional capping may lead to incomplete positioning of the cap on newly synthesized RNA molecules. IVT mRNAs are rapidly emergin...
Pharmaceutics
Pharmaceutics
2022 Jan 29
2,022
[ "Vlatkovic I", "Ludwig J", "Boros G", "Szabó GT", "Reichert J", "Buff M", "Baiersdörfer M", "Reinholz J", "Mahiny AJ", "Şahin U", "Karikó K" ]
Vlatkovic I
Karikó K
[]
[ "Journal Article" ]
14
2
null
pmc-id: PMC8879150;
1999-4923
eng
https://pubmed.ncbi.nlm.nih.gov/35214060/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
35131437
10.1016/j.ymthe.2022.02.001
Lyophilization provides long-term stability for a lipid nanoparticle-formulated, nucleoside-modified mRNA vaccine
Lipid nanoparticle (LNP)-formulated nucleoside-modified mRNA vaccines have proven to be very successful in the fight against the coronavirus disease 2019 (COVID-19) pandemic. They are effective, safe, and can be produced in large quantities. However, the long-term storage of mRNA-LNP vaccines without freezing is still ...
Mol Ther
Molecular therapy : the journal of the American Society of Gene Therapy
2022 May 4
2,022
[ "Muramatsu H", "Lam K", "Bajusz C", "Laczkó D", "Karikó K", "Schreiner P", "Martin A", "Lutwyche P", "Heyes J", "Pardi N" ]
Muramatsu H
Pardi N
[ "Animals", "COVID-19", "Freeze Drying", "Influenza Vaccines", "Liposomes", "Mice", "Nanoparticles", "Nucleosides", "RNA, Messenger", "Vaccines, Synthetic", "mRNA Vaccines" ]
[ "Journal Article", "Research Support, Non-U.S. Gov't", "Research Support, N.I.H., Extramural" ]
30
5
1941-1951
pmc-id: PMC8815268;
1525-0016
eng
https://pubmed.ncbi.nlm.nih.gov/35131437/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
34852217
10.1016/j.immuni.2021.11.001
Lipid nanoparticles enhance the efficacy of mRNA and protein subunit vaccines by inducing robust T follicular helper cell and humoral responses
Adjuvants are critical for improving the quality and magnitude of adaptive immune responses to vaccination. Lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA vaccines have shown great efficacy against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but the mechanism of action of this vaccine ...
Immunity
Immunity
2021 Dec 14
2,021
[ "Alameh MG", "Tombácz I", "Bettini E", "Lederer K", "Sittplangkoon C", "Wilmore JR", "Gaudette BT", "Soliman OY", "Pine M", "Hicks P", "Manzoni TB", "Knox JJ", "Johnson JL", "Laczkó D", "Muramatsu H", "Davis B", "Meng W", "Rosenfeld AM", "Strohmeier S", "Lin PJC", "Mui BL", ...
Alameh MG
Pardi N
[ "Adaptor Proteins, Signal Transducing", "Adjuvants, Immunologic", "Animals", "B-Lymphocytes", "COVID-19", "COVID-19 Vaccines", "Germinal Center", "HEK293 Cells", "Humans", "Immunity, Humoral", "Interleukin-6", "Liposomes", "Mice", "Mice, Inbred BALB C", "Nanoparticles", "Protein Subuni...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
54
12
2877-2892.e7
pmc-id: PMC8566475;
1074-7613
eng
https://pubmed.ncbi.nlm.nih.gov/34852217/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
34580453
10.1038/s41577-021-00608-w
Modified uridines are the key to a successful message
null
Nat Rev Immunol
Nature reviews. Immunology
2021 Oct
2,021
[ "Karikó K" ]
Karikó K
Karikó K
[]
[ "Journal Article" ]
21
10
619
null
1474-1733
eng
https://pubmed.ncbi.nlm.nih.gov/34580453/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
34516826
10.1126/scitranslmed.abc7804
Local delivery of mRNA-encoded cytokines promotes antitumor immunity and tumor eradication across multiple preclinical tumor models
Local immunotherapy ideally stimulates immune responses against tumors while avoiding toxicities associated with systemic administration. Current strategies for tumor-targeted, gene-based delivery, however, are limited by adverse effects such as off-targeting or antivector immunity. We investigated the intratumoral adm...
Sci Transl Med
Science translational medicine
2021 Sep 8
2,021
[ "Hotz C", "Wagenaar TR", "Gieseke F", "Bangari DS", "Callahan M", "Cao H", "Diekmann J", "Diken M", "Grunwitz C", "Hebert A", "Hsu K", "Bernardo M", "Karikó K", "Kreiter S", "Kuhn AN", "Levit M", "Malkova N", "Masciari S", "Pollard J", "Qu H", "Ryan S", "Selmi A", "Schler...
Hotz C
Wiederschain D
[ "Cytokines", "Humans", "Neoplasms", "RNA, Messenger" ]
[ "Journal Article", "Research Support, Non-U.S. Gov't" ]
13
610
eabc7804
null
1946-6234
eng
https://pubmed.ncbi.nlm.nih.gov/34516826/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
34411542
10.1016/j.cels.2021.07.005
What does the success of mRNA vaccines tell us about the future of biological therapeutics?
null
Cell Syst
Cell systems
2021 Aug 18
2,021
[ "Karikó K", "Whitehead K", "van der Meel R" ]
Karikó K
van der Meel R
[ "Vaccines, Synthetic", "mRNA Vaccines" ]
[ "Journal Article" ]
12
8
757-758
pmc-id: PMC8372463;
2405-4712
eng
https://pubmed.ncbi.nlm.nih.gov/34411542/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
34103491
10.1038/s41467-021-23546-6
Nucleoside-modified VEGFC mRNA induces organ-specific lymphatic growth and reverses experimental lymphedema
Lack or dysfunction of the lymphatics leads to secondary lymphedema formation that seriously reduces the function of the affected organs and results in degradation of quality of life. Currently, there is no definitive treatment option for lymphedema. Here, we utilized nucleoside-modified mRNA encapsulated in lipid nano...
Nat Commun
Nature communications
2021 Jun 8
2,021
[ "Szőke D", "Kovács G", "Kemecsei É", "Bálint L", "Szoták-Ajtay K", "Aradi P", "Styevkóné Dinnyés A", "Mui BL", "Tam YK", "Madden TD", "Karikó K", "Kataru RP", "Hope MJ", "Weissman D", "Mehrara BJ", "Pardi N", "Jakus Z" ]
Szőke D
Jakus Z
[ "Animals", "Blood Vessels", "Cell Proliferation", "Diphtheria Toxin", "Disease Models, Animal", "HEK293 Cells", "Humans", "Immunity", "Injections, Intradermal", "Lipids", "Lymphangiogenesis", "Lymphatic Vessels", "Lymphedema", "Mice, Inbred C57BL", "Nanoparticles", "Nucleosides", "Or...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
12
1
3460
pmc-id: PMC8187400;
2041-1723
eng
https://pubmed.ncbi.nlm.nih.gov/34103491/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
34044428
10.1038/s41586-021-03653-6
BNT162b2 vaccine induces neutralizing antibodies and poly-specific T cells in humans
BNT162b2, a nucleoside-modified mRNA formulated in lipid nanoparticles that encodes the SARS-CoV-2 spike glycoprotein (S) stabilized in its prefusion conformation, has demonstrated 95% efficacy in preventing COVID-191. Here we extend a previous phase-I/II trial report2 by presenting data on the immune response induced ...
Nature
Nature
2021 Jul
2,021
[ "Sahin U", "Muik A", "Vogler I", "Derhovanessian E", "Kranz LM", "Vormehr M", "Quandt J", "Bidmon N", "Ulges A", "Baum A", "Pascal KE", "Maurus D", "Brachtendorf S", "Lörks V", "Sikorski J", "Koch P", "Hilker R", "Becker D", "Eller AK", "Grützner J", "Tonigold M", "Boesler ...
Sahin U
Türeci Ö
[ "Adolescent", "Adult", "Antibodies, Neutralizing", "Antibodies, Viral", "BNT162 Vaccine", "CD8-Positive T-Lymphocytes", "COVID-19", "COVID-19 Vaccines", "Epitopes, T-Lymphocyte", "Female", "Humans", "Immunoglobulin G", "Immunologic Memory", "Interferon-gamma", "Interleukin-2", "Male", ...
[ "Clinical Trial, Phase I", "Clinical Trial, Phase II", "Journal Article" ]
595
7868
572-577
null
0028-0836
eng
https://pubmed.ncbi.nlm.nih.gov/34044428/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
33469214
10.1038/s41586-020-03102-w
Publisher Correction: COVID-19 vaccine BNT162b1 elicits human antibody and T(H)1 T cell responses
null
Nature
Nature
2021 Feb
2,021
[ "Sahin U", "Muik A", "Derhovanessian E", "Vogler I", "Kranz LM", "Vormehr M", "Baum A", "Pascal K", "Quandt J", "Maurus D", "Brachtendorf S", "Lörks V", "Sikorski J", "Hilker R", "Becker D", "Eller AK", "Grützner J", "Boesler C", "Rosenbaum C", "Kühnle MC", "Luxemburger U", ...
Sahin U
Türeci Ö
[]
[ "Published Erratum" ]
590
7844
E17
null
0028-0836
eng
https://pubmed.ncbi.nlm.nih.gov/33469214/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
33414215
10.1126/science.aay3638
A noninflammatory mRNA vaccine for treatment of experimental autoimmune encephalomyelitis
The ability to control autoreactive T cells without inducing systemic immune suppression is the major goal for treatment of autoimmune diseases. The key challenge is the safe and efficient delivery of pharmaceutically well-defined antigens in a noninflammatory context. Here, we show that systemic delivery of nanopartic...
Science
Science (New York, N.Y.)
2021 Jan 8
2,021
[ "Krienke C", "Kolb L", "Diken E", "Streuber M", "Kirchhoff S", "Bukur T", "Akilli-Öztürk Ö", "Kranz LM", "Berger H", "Petschenka J", "Diken M", "Kreiter S", "Yogev N", "Waisman A", "Karikó K", "Türeci Ö", "Sahin U" ]
Krienke C
Sahin U
[ "Animals", "Antigen-Presenting Cells", "Autoantigens", "Bystander Effect", "Encephalomyelitis, Autoimmune, Experimental", "Immunosuppression Therapy", "Inflammation", "Mice", "Mice, Inbred C57BL", "Multiple Sclerosis", "Pseudouridine", "RNA, Messenger", "T-Lymphocytes, Regulatory", "Vaccin...
[ "Journal Article", "Research Support, Non-U.S. Gov't" ]
371
6525
145-153
null
0036-8075
eng
https://pubmed.ncbi.nlm.nih.gov/33414215/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
32998157
10.1038/s41586-020-2814-7
COVID-19 vaccine BNT162b1 elicits human antibody and T(H)1 T cell responses
An effective vaccine is needed to halt the spread of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic. Recently, we reported safety, tolerability and antibody response data from an ongoing placebo-controlled, observer-blinded phase I/II coronavirus disease 2019 (COVID-19) vaccine trial with BNT...
Nature
Nature
2020 Oct
2,020
[ "Sahin U", "Muik A", "Derhovanessian E", "Vogler I", "Kranz LM", "Vormehr M", "Baum A", "Pascal K", "Quandt J", "Maurus D", "Brachtendorf S", "Lörks V", "Sikorski J", "Hilker R", "Becker D", "Eller AK", "Grützner J", "Boesler C", "Rosenbaum C", "Kühnle MC", "Luxemburger U", ...
Sahin U
Türeci Ö
[ "Adult", "Antibodies, Neutralizing", "Antibodies, Viral", "CD8-Positive T-Lymphocytes", "COVID-19", "COVID-19 Vaccines", "Coronavirus Infections", "Cytokines", "Female", "Germany", "Humans", "Immunoglobulin G", "Male", "Middle Aged", "Pandemics", "Pneumonia, Viral", "Th1 Cells", "V...
[ "Clinical Trial, Phase I", "Clinical Trial, Phase II", "Journal Article" ]
586
7830
594-599
null
0028-0836
eng
https://pubmed.ncbi.nlm.nih.gov/32998157/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
32783919
10.1016/j.immuni.2020.07.019
A Single Immunization with Nucleoside-Modified mRNA Vaccines Elicits Strong Cellular and Humoral Immune Responses against SARS-CoV-2 in Mice
SARS-CoV-2 infection has emerged as a serious global pandemic. Because of the high transmissibility of the virus and the high rate of morbidity and mortality associated with COVID-19, developing effective and safe vaccines is a top research priority. Here, we provide a detailed evaluation of the immunogenicity of lipid...
Immunity
Immunity
2020 Oct 13
2,020
[ "Laczkó D", "Hogan MJ", "Toulmin SA", "Hicks P", "Lederer K", "Gaudette BT", "Castaño D", "Amanat F", "Muramatsu H", "Oguin TH 3rd", "Ojha A", "Zhang L", "Mu Z", "Parks R", "Manzoni TB", "Roper B", "Strohmeier S", "Tombácz I", "Arwood L", "Nachbagauer R", "Karikó K", "Green...
Laczkó D
Pardi N
[ "Animals", "Antibodies, Neutralizing", "Antibodies, Viral", "B-Lymphocytes", "Betacoronavirus", "CD4-Positive T-Lymphocytes", "CD8-Positive T-Lymphocytes", "COVID-19", "COVID-19 Vaccines", "Coronavirus Infections", "Disease Models, Animal", "Furin", "Humans", "Immunity, Humoral", "Immuni...
[ "Journal Article", "Research Support, N.I.H., Extramural" ]
53
4
724-732.e7
pmc-id: PMC7392193;manuscript-id: NIHMS1617591;
1074-7613
eng
https://pubmed.ncbi.nlm.nih.gov/32783919/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
30974332
10.1016/j.omtn.2019.03.003
Characterization of HIV-1 Nucleoside-Modified mRNA Vaccines in Rabbits and Rhesus Macaques
Despite the enormous effort in the development of effective vaccines against HIV-1, no vaccine candidate has elicited broadly neutralizing antibodies in humans. Thus, generation of more effective anti-HIV vaccines is critically needed. Here we characterize the immune responses induced by nucleoside-modified and purifie...
Mol Ther Nucleic Acids
Molecular therapy. Nucleic acids
2019 Apr 15
2,019
[ "Pardi N", "LaBranche CC", "Ferrari G", "Cain DW", "Tombácz I", "Parks RJ", "Muramatsu H", "Mui BL", "Tam YK", "Karikó K", "Polacino P", "Barbosa CJ", "Madden TD", "Hope MJ", "Haynes BF", "Montefiori DC", "Hu SL", "Weissman D" ]
Pardi N
Weissman D
[]
[ "Journal Article" ]
15
null
36-47
pmc-id: PMC6454128;
2162-2531
eng
https://pubmed.ncbi.nlm.nih.gov/30974332/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
30933724
10.1016/j.omtn.2019.02.018
A Facile Method for the Removal of dsRNA Contaminant from In Vitro-Transcribed mRNA
The increasing importance of in vitro-transcribed (IVT) mRNA for synthesizing the encoded therapeutic protein in vivo demands the manufacturing of pure mRNA products. The major contaminant in the IVT mRNA is double-stranded RNA (dsRNA), a transcriptional by-product that can be removed only by burdensome procedure requi...
Mol Ther Nucleic Acids
Molecular therapy. Nucleic acids
2019 Apr 15
2,019
[ "Baiersdörfer M", "Boros G", "Muramatsu H", "Mahiny A", "Vlatkovic I", "Sahin U", "Karikó K" ]
Baiersdörfer M
Karikó K
[]
[ "Journal Article" ]
15
null
26-35
pmc-id: PMC6444222;
2162-2531
eng
https://pubmed.ncbi.nlm.nih.gov/30933724/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
30905578
10.1016/j.ymthe.2019.03.009
In vitro-Transcribed mRNA Therapeutics: Out of the Shadows and Into the Spotlight
null
Mol Ther
Molecular therapy : the journal of the American Society of Gene Therapy
2019 Apr 10
2,019
[ "Karikó K" ]
Karikó K
Karikó K
[ "Cancer Vaccines", "Drug Delivery Systems", "Genetic Therapy", "Humans", "In Vitro Techniques", "RNA, Messenger", "Transcription, Genetic" ]
[ "Editorial" ]
27
4
691-692
pmc-id: PMC6453554;
1525-0016
eng
https://pubmed.ncbi.nlm.nih.gov/30905578/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
30819612
10.1016/j.ymthe.2019.01.018
The Emerging Role of In Vitro-Transcribed mRNA in Adoptive T Cell Immunotherapy
Adoptive T cell therapy is a form of cellular therapy that utilizes human immune cells, often empowered by the expression of recombinant proteins, to attack selected targets present on tumor or infected cells. T cell-based immunotherapy has been progressing over the past several decades, and reached a milestone with th...
Mol Ther
Molecular therapy : the journal of the American Society of Gene Therapy
2019 Apr 10
2,019
[ "Foster JB", "Barrett DM", "Karikó K" ]
Foster JB
Karikó K
[ "Animals", "Cell- and Tissue-Based Therapy", "Gene Editing", "Genetic Vectors", "Humans", "Immunotherapy, Adoptive", "In Vitro Techniques", "Lymphocytes, Tumor-Infiltrating", "Mice", "Neoplasms", "RNA, Messenger", "Receptors, Antigen, T-Cell", "Receptors, Chimeric Antigen", "T-Lymphocytes"...
[ "Journal Article", "Review" ]
27
4
747-756
pmc-id: PMC6453504;
1525-0016
eng
https://pubmed.ncbi.nlm.nih.gov/30819612/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
30135514
10.1038/s41467-018-05482-0
Nucleoside-modified mRNA immunization elicits influenza virus hemagglutinin stalk-specific antibodies
Currently available influenza virus vaccines have inadequate effectiveness and are reformulated annually due to viral antigenic drift. Thus, development of a vaccine that confers long-term protective immunity against antigenically distant influenza virus strains is urgently needed. The highly conserved influenza virus ...
Nat Commun
Nature communications
2018 Aug 22
2,018
[ "Pardi N", "Parkhouse K", "Kirkpatrick E", "McMahon M", "Zost SJ", "Mui BL", "Tam YK", "Karikó K", "Barbosa CJ", "Madden TD", "Hope MJ", "Krammer F", "Hensley SE", "Weissman D" ]
Pardi N
Weissman D
[ "Animals", "Antibodies, Viral", "Cells, Cultured", "Dogs", "Enzyme-Linked Immunosorbent Assay", "Female", "Ferrets", "Flow Cytometry", "Hemagglutinins", "Influenza A Virus, H5N1 Subtype", "Madin Darby Canine Kidney Cells", "Mice", "Mice, Inbred BALB C", "Orthomyxoviridae", "Phylogeny", ...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
9
1
3361
pmc-id: PMC6105651;
2041-1723
eng
https://pubmed.ncbi.nlm.nih.gov/30135514/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
30024272
10.1089/hum.2018.145
Purification of mRNA Encoding Chimeric Antigen Receptor Is Critical for Generation of a Robust T-Cell Response
T cells made with messenger RNA (mRNA) encoding chimeric antigen receptor (CAR) offer a safe alternative to those transduced with viral CARs by mitigating the side effects of constitutively active T cells. Previous studies have shown that mRNA CAR T cells are transiently effective but lack persistence and potency acros...
Hum Gene Ther
Human gene therapy
2019 Feb
2,019
[ "Foster JB", "Choudhari N", "Perazzelli J", "Storm J", "Hofmann TJ", "Jain P", "Storm PB", "Pardi N", "Weissman D", "Waanders AJ", "Grupp SA", "Karikó K", "Resnick AC", "Barrett DM" ]
Foster JB
Barrett DM
[ "Adoptive Transfer", "Animals", "Antigens, CD19", "Cell Line, Tumor", "Electroporation", "Humans", "Leukemia", "Mice", "Mice, Inbred NOD", "Mice, Knockout", "Mice, SCID", "RNA, Messenger", "Receptors, Chimeric Antigen", "T-Lymphocytes", "Xenograft Model Antitumor Assays" ]
[ "Journal Article", "Research Support, Non-U.S. Gov't" ]
30
2
168-178
pmc-id: PMC6383579;
1043-0342
eng
https://pubmed.ncbi.nlm.nih.gov/30024272/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
29739835
10.1084/jem.20171450
Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses
T follicular helper (Tfh) cells are required to develop germinal center (GC) responses and drive immunoglobulin class switch, affinity maturation, and long-term B cell memory. In this study, we characterize a recently developed vaccine platform, nucleoside-modified, purified mRNA encapsulated in lipid nanoparticles (mR...
J Exp Med
The Journal of experimental medicine
2018 Jun 4
2,018
[ "Pardi N", "Hogan MJ", "Naradikian MS", "Parkhouse K", "Cain DW", "Jones L", "Moody MA", "Verkerke HP", "Myles A", "Willis E", "LaBranche CC", "Montefiori DC", "Lobby JL", "Saunders KO", "Liao HX", "Korber BT", "Sutherland LL", "Scearce RM", "Hraber PT", "Tombácz I", "Muramat...
Pardi N
Weissman D
[ "Adjuvants, Immunologic", "Animals", "Antibodies, Neutralizing", "Antibody Formation", "Antigens", "B-Lymphocytes", "Germinal Center", "Lipids", "Macaca mulatta", "Nanoparticles", "Nucleosides", "Protein Subunits", "RNA, Messenger", "T-Lymphocytes, Helper-Inducer", "Time Factors", "Vac...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't", "Research Support, U.S. Gov't, Non-P.H.S." ]
215
6
1571-1588
pmc-id: PMC5987916;
0022-1007
eng
https://pubmed.ncbi.nlm.nih.gov/29739835/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
29733456
10.1002/JLB.3AB1117-455R
Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation
The asthmatic airways are highly susceptible to inflammatory injury by air pollutants such as ozone (O3 ), characterized by enhanced activation of eosinophilic granulocytes and a failure of immune protective mechanisms. Eosinophil activation during asthma exacerbation contributes to the proinflammatory oxidative stress...
J Leukoc Biol
Journal of leukocyte biology
2018 Jul
2,018
[ "Yousefi S", "Sharma SK", "Stojkov D", "Germic N", "Aeschlimann S", "Ge MQ", "Flayer CH", "Larson ED", "Redai IG", "Zhang S", "Koziol-White CJ", "Karikó K", "Simon HU", "Haczku A" ]
Yousefi S
Haczku A
[ "Animals", "Asthma", "Cells, Cultured", "Eosinophils", "Extracellular Traps", "Humans", "Hypersensitivity", "Mice", "Oxidants, Photochemical", "Oxidative Stress", "Ozone", "Pulmonary Surfactant-Associated Protein D" ]
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
104
1
205-214
pmc-id: PMC6450398;manuscript-id: NIHMS977812;
0741-5400
eng
https://pubmed.ncbi.nlm.nih.gov/29733456/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
28985209
10.1038/nm1017-1241d
Erratum: Elimination of large tumors in mice by mRNA-encoded bispecific antibodies
This corrects the article DOI: 10.1038/nm.4356.
Nat Med
Nature medicine
2017 Oct 6
2,017
[ "Stadler CR", "Bähr-Mahmud H", "Celik L", "Hebich B", "Roth AS", "Roth RP", "Karikó K", "Türeci Ö", "Sahin U" ]
Stadler CR
Sahin U
[]
[ "Journal Article", "Published Erratum" ]
23
10
1241
null
1078-8956
eng
https://pubmed.ncbi.nlm.nih.gov/28985209/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
28604701
10.1038/nm.4356
Elimination of large tumors in mice by mRNA-encoded bispecific antibodies
The potential of bispecific T cell-engaging antibodies is hindered by manufacturing challenges and short serum half-life. We circumvented these limitations by treating mice with in vitro-transcribed pharmacologically optimized, nucleoside-modified mRNA encoding the antibody. We achieved sustained endogenous synthesis o...
Nat Med
Nature medicine
2017 Jul
2,017
[ "Stadler CR", "Bähr-Mahmud H", "Celik L", "Hebich B", "Roth AS", "Roth RP", "Karikó K", "Türeci Ö", "Sahin U" ]
Stadler CR
Sahin U
[ "Animals", "Antibodies, Bispecific", "Cell Line, Tumor", "Cytokines", "Enzyme-Linked Immunosorbent Assay", "Female", "Humans", "Immunoblotting", "Immunohistochemistry", "In Vitro Techniques", "Luminescent Measurements", "Male", "Mice", "Mice, Inbred BALB C", "Mice, Inbred NOD", "Neopla...
[ "Journal Article" ]
23
7
815-817
null
1078-8956
eng
https://pubmed.ncbi.nlm.nih.gov/28604701/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
28251988
10.1038/ncomms14630
Administration of nucleoside-modified mRNA encoding broadly neutralizing antibody protects humanized mice from HIV-1 challenge
Monoclonal antibodies are one of the fastest growing classes of pharmaceutical products, however, their potential is limited by the high cost of development and manufacturing. Here we present a safe and cost-effective platform for in vivo expression of therapeutic antibodies using nucleoside-modified mRNA. To demonstra...
Nat Commun
Nature communications
2017 Mar 2
2,017
[ "Pardi N", "Secreto AJ", "Shan X", "Debonera F", "Glover J", "Yi Y", "Muramatsu H", "Ni H", "Mui BL", "Tam YK", "Shaheen F", "Collman RG", "Karikó K", "Danet-Desnoyers GA", "Madden TD", "Hope MJ", "Weissman D" ]
Pardi N
Weissman D
[ "Animals", "Antibodies, Monoclonal", "Antibodies, Neutralizing", "Broadly Neutralizing Antibodies", "Drug Administration Schedule", "Female", "HIV Antibodies", "HIV Infections", "HIV-1", "Humans", "Immunization, Passive", "Lipids", "Mice", "Mice, Inbred BALB C", "Mice, Inbred C57BL", "...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
8
null
14630
pmc-id: PMC5337964;
2041-1723
eng
https://pubmed.ncbi.nlm.nih.gov/28251988/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
28151488
10.1038/nature21428
Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination
Zika virus (ZIKV) has recently emerged as a pandemic associated with severe neuropathology in newborns and adults. There are no ZIKV-specific treatments or preventatives. Therefore, the development of a safe and effective vaccine is a high priority. Messenger RNA (mRNA) has emerged as a versatile and highly effective p...
Nature
Nature
2017 Mar 9
2,017
[ "Pardi N", "Hogan MJ", "Pelc RS", "Muramatsu H", "Andersen H", "DeMaso CR", "Dowd KA", "Sutherland LL", "Scearce RM", "Parks R", "Wagner W", "Granados A", "Greenhouse J", "Walker M", "Willis E", "Yu JS", "McGee CE", "Sempowski GD", "Mui BL", "Tam YK", "Huang YJ", "Vanlandin...
Pardi N
Weissman D
[ "Animals", "Antibodies, Neutralizing", "Antibodies, Viral", "Antigens, Viral", "Female", "Glycoproteins", "Injections, Intradermal", "Macaca mulatta", "Mice", "Mice, Inbred BALB C", "Mice, Inbred C57BL", "Nanoparticles", "RNA Stability", "RNA, Messenger", "RNA, Viral", "Time Factors", ...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, N.I.H., Intramural", "Research Support, Non-U.S. Gov't" ]
543
7644
248-251
pmc-id: PMC5344708;manuscript-id: NIHMS847615;
0028-0836
eng
https://pubmed.ncbi.nlm.nih.gov/28151488/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
27236808
10.1007/978-1-4939-3625-0_20
Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA
In vitro-transcribed (IVT) mRNA encoding therapeutic protein has the potential to treat a variety of diseases by serving as template for translation in the patient. To optimize conditions for such therapy, reporter protein-encoding mRNAs are usually used. One preferred reporter is erythropoietin (EPO), which stimulates...
Methods Mol Biol
Methods in molecular biology (Clifton, N.J.)
2016
2,016
[ "Mahiny AJ", "Karikó K" ]
Mahiny AJ
Karikó K
[ "Animals", "Erythrocytes", "Erythropoietin", "Hematocrit", "Humans", "In Vitro Techniques", "Mice", "Mice, Inbred BALB C", "RNA, Messenger" ]
[ "Journal Article" ]
1428
null
297-306
null
1064-3745
eng
https://pubmed.ncbi.nlm.nih.gov/27236808/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
27236802
10.1007/978-1-4939-3625-0_14
Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage
In vitro-synthesized mRNA containing nucleoside modifications has great therapeutical potential to transiently express proteins with physiological importance. One such protein is photolyase which rapidly removes UV-induced DNA damages, but this enzyme is absent in humans. Here, we apply a novel mRNA-based platform to a...
Methods Mol Biol
Methods in molecular biology (Clifton, N.J.)
2016
2,016
[ "Boros G", "Karikó K", "Muramatsu H", "Miko E", "Emri E", "Hegedűs C", "Emri G", "Remenyik É" ]
Boros G
Remenyik É
[ "Cell Line", "DNA", "DNA Damage", "DNA Repair", "Deoxyribodipyrimidine Photo-Lyase", "Humans", "Keratinocytes", "Nucleosides", "RNA, Messenger", "Transfection" ]
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
1428
null
219-28
null
1064-3745
eng
https://pubmed.ncbi.nlm.nih.gov/27236802/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
26321183
10.1038/mt.2015.138
mRNA: Fulfilling the Promise of Gene Therapy
null
Mol Ther
Molecular therapy : the journal of the American Society of Gene Therapy
2015 Sep
2,015
[ "Weissman D", "Karikó K" ]
Weissman D
Karikó K
[ "Animals", "Gene Expression", "Genetic Therapy", "Humans", "RNA, Messenger" ]
[ "Journal Article", "Comment" ]
23
9
1416-7
pmc-id: PMC4817894;
1525-0016
eng
https://pubmed.ncbi.nlm.nih.gov/26321183/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
26264835
10.1016/j.jconrel.2015.08.007
Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes
In recent years, in vitro transcribed messenger RNA (mRNA) has emerged as a potential therapeutic platform. To fulfill its promise, effective delivery of mRNA to specific cell types and tissues needs to be achieved. Lipid nanoparticles (LNPs) are efficient carriers for short-interfering RNAs and have entered clinical t...
J Control Release
Journal of controlled release : official journal of the Controlled Release Society
2015 Nov 10
2,015
[ "Pardi N", "Tuyishime S", "Muramatsu H", "Kariko K", "Mui BL", "Tam YK", "Madden TD", "Hope MJ", "Weissman D" ]
Pardi N
Weissman D
[ "Animals", "Cells, Cultured", "Dendritic Cells", "Female", "HEK293 Cells", "Humans", "Kinetics", "Luciferases, Firefly", "Lung", "Mice, Inbred BALB C", "Nanoparticles", "Phosphatidylethanolamines", "Pseudouridine", "RNA, Messenger", "Transfection" ]
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
217
null
345-51
pmc-id: PMC4624045;manuscript-id: NIHMS723881;
0168-3659
eng
https://pubmed.ncbi.nlm.nih.gov/26264835/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
26121660
10.1371/journal.pone.0131141
Identification of Cyclobutane Pyrimidine Dimer-Responsive Genes Using UVB-Irradiated Human Keratinocytes Transfected with In Vitro-Synthesized Photolyase mRNA
Major biological effects of UVB are attributed to cyclobutane pyrimidine dimers (CPDs), the most common photolesions formed on DNA. To investigate the contribution of CPDs to UVB-induced changes of gene expression, a model system was established by transfecting keratinocytes with pseudouridine-modified mRNA (Ψ-mRNA) en...
PLoS One
PloS one
2015
2,015
[ "Boros G", "Miko E", "Muramatsu H", "Weissman D", "Emri E", "van der Horst GT", "Szegedi A", "Horkay I", "Emri G", "Karikó K", "Remenyik É" ]
Boros G
Remenyik É
[ "Animals", "Cell Line", "Cyclin E", "Cyclin-Dependent Kinase Inhibitor p15", "DNA Repair", "Deoxyribodipyrimidine Photo-Lyase", "Gene Expression Regulation", "Humans", "JNK Mitogen-Activated Protein Kinases", "Keratinocytes", "MAP Kinase Signaling System", "Oligonucleotide Array Sequence Analy...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
10
6
e0131141
pmc-id: PMC4488231;
1932-6203
eng
https://pubmed.ncbi.nlm.nih.gov/26121660/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
25233993
10.1038/nrd4278
mRNA-based therapeutics--developing a new class of drugs
In vitro transcribed (IVT) mRNA has recently come into focus as a potential new drug class to deliver genetic information. Such synthetic mRNA can be engineered to transiently express proteins by structurally resembling natural mRNA. Advances in addressing the inherent challenges of this drug class, particularly relate...
Nat Rev Drug Discov
Nature reviews. Drug discovery
2014 Oct
2,014
[ "Sahin U", "Karikó K", "Türeci Ö" ]
Sahin U
Türeci Ö
[ "Animals", "Drug Delivery Systems", "Drug Design", "Humans", "Immunotherapy", "Pluripotent Stem Cells", "Proteins", "RNA, Messenger" ]
[ "Journal Article", "Review" ]
13
10
759-80
null
1474-1776
eng
https://pubmed.ncbi.nlm.nih.gov/25233993/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
24211294
10.1016/j.jphotobiol.2013.09.010
Transfection of pseudouridine-modified mRNA encoding CPD-photolyase leads to repair of DNA damage in human keratinocytes: a new approach with future therapeutic potential
UVB irradiation induces harmful photochemical reactions, including formation of Cyclobutane Pyrimidine Dimers (CPDs) in DNA. Accumulation of unrepaired CPD lesions causes inflammation, premature ageing and skin cancer. Photolyases are DNA repair enzymes that can rapidly restore DNA integrity in a light-dependent proces...
J Photochem Photobiol B
Journal of photochemistry and photobiology. B, Biology
2013 Dec 5
2,013
[ "Boros G", "Miko E", "Muramatsu H", "Weissman D", "Emri E", "Rózsa D", "Nagy G", "Juhász A", "Juhász I", "van der Horst G", "Horkay I", "Remenyik É", "Karikó K", "Emri G" ]
Boros G
Emri G
[ "Animals", "Cell Line", "Cell Proliferation", "DNA Repair", "Deoxyribodipyrimidine Photo-Lyase", "Humans", "Interleukin-6", "Keratinocytes", "Light", "Lipids", "Potoroidae", "Pseudouridine", "Pyrimidine Dimers", "RNA, Messenger", "Transfection", "Ultraviolet Rays" ]
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
129
null
93-9
pmc-id: PMC3888937;manuscript-id: NIHMS538757;
1011-1344
eng
https://pubmed.ncbi.nlm.nih.gov/24211294/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
23296926
10.1007/978-1-62703-260-5_3
HPLC purification of in vitro transcribed long RNA
In vitro transcription of DNA with phage RNA polymerases is currently the most efficient method to produce long sequence-specific RNA. While the reaction can yield large quantities of RNA, it contains impurities due to various unwanted activities of the polymerases. Here, we described an easily performed HPLC purificat...
Methods Mol Biol
Methods in molecular biology (Clifton, N.J.)
2013
2,013
[ "Weissman D", "Pardi N", "Muramatsu H", "Karikó K" ]
Weissman D
Karikó K
[ "Chromatography, High Pressure Liquid", "DNA", "DNA-Directed RNA Polymerases", "Nucleosides", "RNA", "Transcription, Genetic" ]
[ "Journal Article", "Research Support, N.I.H., Extramural" ]
969
null
43-54
null
1064-3745
eng
https://pubmed.ncbi.nlm.nih.gov/23296926/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
23296925
10.1007/978-1-62703-260-5_2
In vitro transcription of long RNA containing modified nucleosides
The in vitro synthesis of long RNA can be accomplished using phage RNA polymerase and template DNA. However, the in vitro synthesized RNA, unlike those transcribed in vivo in cells, lacks nucleoside modifications. Introducing modified nucleosides into in vitro transcripts is important because they reduce the potential ...
Methods Mol Biol
Methods in molecular biology (Clifton, N.J.)
2013
2,013
[ "Pardi N", "Muramatsu H", "Weissman D", "Karikó K" ]
Pardi N
Karikó K
[ "Animals", "Bacteriophages", "Cell Line", "DNA-Directed RNA Polymerases", "Genetic Engineering", "Humans", "Nucleosides", "RNA", "Transfection", "Viral Proteins" ]
[ "Journal Article", "Research Support, N.I.H., Extramural", "Review" ]
969
null
29-42
null
1064-3745
eng
https://pubmed.ncbi.nlm.nih.gov/23296925/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
22334017
10.1038/mt.2012.7
Increased erythropoiesis in mice injected with submicrogram quantities of pseudouridine-containing mRNA encoding erythropoietin
Advances in the optimization of in vitro-transcribed mRNA are bringing mRNA-mediated therapy closer to reality. In cultured cells, we recently achieved high levels of translation with high-performance liquid chromatography (HPLC)-purified, in vitro-transcribed mRNAs containing the modified nucleoside pseudouridine. Imp...
Mol Ther
Molecular therapy : the journal of the American Society of Gene Therapy
2012 May
2,012
[ "Karikó K", "Muramatsu H", "Keller JM", "Weissman D" ]
Karikó K
Weissman D
[ "Animals", "Cell Count", "Cell Proliferation", "Chromatography, High Pressure Liquid", "Drug Delivery Systems", "Erythropoiesis", "Erythropoietin", "Female", "Genetic Vectors", "Hematocrit", "Humans", "Injections, Intraperitoneal", "Macaca mulatta", "Mice", "Mice, Inbred BALB C", "Pseu...
[ "Journal Article", "Research Support, N.I.H., Extramural" ]
20
5
948-53
pmc-id: PMC3345990;
1525-0016
eng
https://pubmed.ncbi.nlm.nih.gov/22334017/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
22301437
10.1038/gt.2012.5
Nucleofection induces transient eIF2α phosphorylation by GCN2 and PERK
Nucleofection permits efficient transfection even with difficult cell types such as primary and non-dividing cells, and is used to deliver various nucleic acids, including DNA, mRNA, and small interfering RNA. Unlike DNA and small interfering RNA, mRNA is subject to rapid degradation, which necessitates instant early t...
Gene Ther
Gene therapy
2013 Feb
2,013
[ "Anderson BR", "Karikó K", "Weissman D" ]
Anderson BR
Weissman D
[ "Animals", "Cell Line", "Eukaryotic Initiation Factor-2", "Humans", "Mice", "Phosphorylation", "Protein Serine-Threonine Kinases", "Transfection", "eIF-2 Kinase" ]
[ "Journal Article", "Research Support, N.I.H., Extramural" ]
20
2
136-42
pmc-id: PMC3345295;manuscript-id: NIHMS332400;
0969-7128
eng
https://pubmed.ncbi.nlm.nih.gov/22301437/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
22028224
10.1002/ana.22528
Toll-like receptor 4 contributes to poor outcome after intracerebral hemorrhage
Intracerebral hemorrhage (ICH) is a devastating stroke subtype in which perihematomal inflammation contributes to neuronal injury and functional disability. Histologically, the region becomes infiltrated with neutrophils and activated microglia followed by neuronal loss, but little is known about the immune signals tha...
Ann Neurol
Annals of neurology
2011 Oct
2,011
[ "Sansing LH", "Harris TH", "Welsh FA", "Kasner SE", "Hunter CA", "Kariko K" ]
Sansing LH
Kariko K
[ "Animals", "Brain Injuries", "Cerebral Hemorrhage", "Inflammation", "Male", "Mice", "Mice, Inbred C3H", "Mice, Knockout", "Severity of Illness Index", "Signal Transduction", "Toll-Like Receptor 4" ]
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
70
4
646-56
pmc-id: PMC3671585;manuscript-id: NIHMS469371;
0364-5134
eng
https://pubmed.ncbi.nlm.nih.gov/22028224/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
21890902
10.1093/nar/gkr695
Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA
In vitro-transcribed mRNA has great therapeutic potential to transiently express the encoded protein without the adverse effects of viral and DNA-based constructs. Mammalian cells, however, contain RNA sensors of the innate immune system that must be considered in the generation of therapeutic RNA. Incorporation of mod...
Nucleic Acids Res
Nucleic acids research
2011 Nov
2,011
[ "Karikó K", "Muramatsu H", "Ludwig J", "Weissman D" ]
Karikó K
Weissman D
[ "Animals", "Cells, Cultured", "Chromatography, High Pressure Liquid", "Cytidine", "Genetic Therapy", "HEK293 Cells", "Humans", "Mice", "Protein Biosynthesis", "Pseudouridine", "RNA, Double-Stranded", "RNA, Messenger", "Transcription, Genetic", "Transfection" ]
[ "Journal Article", "Research Support, N.I.H., Extramural" ]
39
21
e142
pmc-id: PMC3241667;
0305-1048
eng
https://pubmed.ncbi.nlm.nih.gov/21890902/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
21876533
10.3791/2618
Autologous blood injection to model spontaneous intracerebral hemorrhage in mice
Investigation of the pathophysiology of injury after intracerebral hemorrhage (ICH) requires a reproducible animal model. While ICH accounts for 10-15% of all strokes, there remains no specific effective therapy. The autologous blood injection model in mice involves the stereotaxic injection of arterial blood into the ...
J Vis Exp
Journal of visualized experiments : JoVE
2011 Aug 24
2,011
[ "Sansing LH", "Kasner SE", "McCullough L", "Agarwal P", "Welsh FA", "Kariko K" ]
Sansing LH
Kariko K
[ "Animals", "Basal Ganglia", "Blood", "Cerebral Hemorrhage", "Disease Models, Animal", "Mice", "Wounds and Injuries" ]
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't", "Video-Audio Media" ]
null
54
null
pmc-id: PMC3217617;
1940-087X
eng
https://pubmed.ncbi.nlm.nih.gov/21876533/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
21813458
10.1093/nar/gkr586
Nucleoside modifications in RNA limit activation of 2'-5'-oligoadenylate synthetase and increase resistance to cleavage by RNase L
The interferon-induced enzymes 2'-5'-oligoadenylate synthetase (OAS) and RNase L are key components of innate immunity involved in sensory and effector functions following viral infections. Upon binding target RNA, OAS is activated to produce 2'-5'-linked oligoadenylates (2-5A) that activate RNase L, which then cleaves...
Nucleic Acids Res
Nucleic acids research
2011 Nov
2,011
[ "Anderson BR", "Muramatsu H", "Jha BK", "Silverman RH", "Weissman D", "Karikó K" ]
Anderson BR
Karikó K
[ "2',5'-Oligoadenylate Synthetase", "Animals", "Cells, Cultured", "Endoribonucleases", "Half-Life", "Humans", "Mice", "Nucleosides", "Protein Biosynthesis", "Pseudouridine", "RNA", "RNA, Ribosomal", "Uridine" ]
[ "Journal Article", "Research Support, N.I.H., Extramural" ]
39
21
9329-38
pmc-id: PMC3241635;
0305-1048
eng
https://pubmed.ncbi.nlm.nih.gov/21813458/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
21725751
10.1007/978-3-7091-0693-8_29
Neutrophil depletion diminishes monocyte infiltration and improves functional outcome after experimental intracerebral hemorrhage
Inflammation contributes to secondary injury and neuronal loss after intracerebral hemorrhage, but the role of individual immune populations in these processes is unclear. In a mouse model, the injection of autologous blood into the striatum was associated with an intense inflammatory cell infiltrate composed of neutro...
Acta Neurochir Suppl
Acta neurochirurgica. Supplement
2011
2,011
[ "Sansing LH", "Harris TH", "Kasner SE", "Hunter CA", "Kariko K" ]
Sansing LH
Kariko K
[ "Analysis of Variance", "Animals", "Antibodies, Monoclonal", "Antigens, Ly", "CD11b Antigen", "CD11c Antigen", "Cerebral Hemorrhage", "Dendritic Cells", "Disease Models, Animal", "Functional Laterality", "Inflammation", "Lymphocyte Count", "Male", "Mice", "Mice, Inbred C3H", "Monocytes...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
111
null
173-8
pmc-id: PMC3702167;manuscript-id: NIHMS470688;
0065-1419
eng
https://pubmed.ncbi.nlm.nih.gov/21725751/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc
21297615
10.1038/nature09830
DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration
Geographic atrophy (GA), an untreatable advanced form of age-related macular degeneration, results from retinal pigmented epithelium (RPE) cell degeneration. Here we show that the microRNA (miRNA)-processing enzyme DICER1 is reduced in the RPE of humans with GA, and that conditional ablation of Dicer1, but not seven ot...
Nature
Nature
2011 Mar 17
2,011
[ "Kaneko H", "Dridi S", "Tarallo V", "Gelfand BD", "Fowler BJ", "Cho WG", "Kleinman ME", "Ponicsan SL", "Hauswirth WW", "Chiodo VA", "Karikó K", "Yoo JW", "Lee DK", "Hadziahmetovic M", "Song Y", "Misra S", "Chaudhuri G", "Buaas FW", "Braun RE", "Hinton DR", "Zhang Q", "Gross...
Kaneko H
Ambati J
[ "Alu Elements", "Animals", "Cell Death", "Cell Survival", "Cells, Cultured", "DEAD-box RNA Helicases", "Gene Knockdown Techniques", "Humans", "Macular Degeneration", "Mice", "MicroRNAs", "Molecular Sequence Data", "Oligonucleotides, Antisense", "Phenotype", "RNA", "Retinal Pigment Epit...
[ "Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't" ]
471
7338
325-30
pmc-id: PMC3077055;manuscript-id: NIHMS265989;
0028-0836
eng
https://pubmed.ncbi.nlm.nih.gov/21297615/
93XkJrUvfYyutHSZS
ehrTjgKQjCkT4usrc