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Another netizen:”Recently us Beijing men really are very proud of ourself.Hero Yang,one of us,killed six Shanghai policemen with a single blade.What a beautiful and sweet feeling of revenge.This few years,we are sick of petty and sissy Shanghai men the like of Yao Ming and Liu Xiang. I suggest full pardon for Hero Yang,and vote him into the central government.” |
To JK, |
The above post was on your another thread “Citizen journalists prepare for the Olympic” but score no response. |
I put it up on here again hopefully someone might bother to send in a few comments,for or against it,whatever. |
• alibaba |
@alibaba: |
This natonal anthem written for this Yan Zhao area hero, represent what most chinese think, not just people from Yang Jia’s hometown. |
These comments you posted as dialogs between shanghai and beijing netizens, what are the purpose of that? The best will be painting a picture of beijingers rude rivary discredit Yang Jia case in legal sense. |
We all know, Beijing has many rivals, shanghai is not one of them. To stur a scene like a fight between Beijing and Shanghai hoping for Beijing’s international rivals (by acting like a rival of Beijing)helping win sympathy for cops is something new this time. |
• purpose |
I can’t believe how low this is yet to go to turn this case into a seemingly regional conflict to discredit the legal sense of it. |
• ali baba |
To alibaba: |
Are you going to continue using this name “alibaba” ? |
If you are,I can change to another name,otherwise is a bit of confusion. |
I am glad you like the new song of the guerrilla.I will post more of them because it shows that people do have talents. |
• purpose |
If you are one of those 50-cents party who rely earnings through clicks, thanks, I still can donate though. |
I didn’t commnent on song of the guerrilla, you seem to call the writers of this song guerrillas. Regardless how much you want to prove the “talents” of guerrillas you disagree with, it is still a legal case that has been handled very poorly. |
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Recombinant Human MIF (carrier-free) |
Pricing & Availability |
Other Names |
Macrophage migration inhibitory factor, GIF, GLIF, MMIF |
Ave. Rating |
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Product Citations |
publications |
Cat # Size Price Quantity Avail. Save |
599404 25 µg $185 |
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599406 100 µg $585 |
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599408 500 µg $1,695 |
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Description |
MIF was discovered in the 1960’s as a T-lymphocyte product that inhibits the random migration of macrophages during delayed-type hypersensitivity responses. This cytokine is a single, non-glycosylated, 115 amino acids polypeptide that has a β-α-β motif. It is known that MIF induces inflammatory cytokines, nitric oxide and superoxide anions, and regulates macrophage and lymphocyte proliferation. The immunoregulatory activities of MIF are based upon transcriptional regulation of inflammatory gene products, modulation of cell proliferation and cell cycle inhibition of p53-mediated apoptosis, and a number of metabolic effects. MIF also demonstrates broad regulatory properties and is considered as a critical mediator of multiple disorders, including inflammatory and autoimmune diseases such as rheumatoid arthritis, glomerulonephritis, diabetes, atherosclerosis, sepsis, asthma, and acute respiratory distress syndrome. Furthermore, studies have highlighted the role of MIF in tumorigenesis. Human cancer tissues, including skin, brain, breast, colon, prostate, and lung-derived tumors overexpress MIF; MIF levels correlated with tumor aggressiveness and metastatic potential. Therefore, MIF is considered a viable therapeutic target for treating inflammatory diseases and neoplasia. In addition to its physiologic and pathophysiologic activities, MIF is known to act as a tautomerase and has a catalytically active N-terminal proline that is invariant in structurally homologous bacterial isomerases. Although the relationship between the catalytic activity and biological function of MIF is not yet fully understood, targeting MIF tautomerase activity using small-molecule inhibitors has emerged as an attractive strategy for inhibiting MIF proinflammatory activity and attenuating its biological activity in vitro and in vivo. Human and mouse MIF share a 90% homology. |
Product Details |
Technical data sheet |
Product Details |
Reactivity |
Human |
Source |
Human MIF, amino acids (Pro2-Ala115) (Accession# NP_002406.1), was expressed in E. coli with an additional N-terminal Met. |
Molecular Mass |
The 115 amino acid recombinant protein has a predicted molecular mass of approximately 12.5 kD. The protein migrates above 14 kD by SDS-PAGE in DTT-reducing conditions and above 12.5 kD in non-reducing conditions. The predicted N-terminal amino acid is Met. |
Purity |
>95%, as determined by Coomassie stained SDS-PAGE. |
Formulation |
0.22 µm filtered protein solution is in PBS. |
Endotoxin Level |
Less than 0.1 EU per µg of protein as determine by the LAL method. |
Concentration |
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.) |
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration. |
Storage & Handling |
Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored between 2°C and 8°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles. |
Application |
Bioassay |
Application Notes |
We currently check the quality of this recombinant protein by purity, endotoxin level and molecular weight. At this time, we do not have bioassay system established yet for testing this protein. However, we are continuing to seek out options to test its activity. |
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com. |
Antigen Details |
Structure |
Monomer |
Distribution |
Macrophages, eosinophils, T cells, pituitary gland. |
Function |
MIF stimulates IL-1, IL-8, and MMP expression on fibroblasts. It also stimulates NO and TNF-alpha production on macrophages. MIF regulates the migration of macrophages. The secretion of MIF is regulated by steroids. |
Interaction |
Macrophages, fibroblasts |
Ligand/Receptor |
CD74 |
Bioactivity |
Human MIF inhibits the migration of THP-1 cells in the presence of human MCP-1 |
Biology Area |
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity |
Molecular Family |
Cytokines/Chemokines |
Antigen References |
1. David JR. 1966. Proc. Natl. Acad. Sci. 56:72. |
2. Hare AA, et al. 2010. Bioorg. Med. Chem. Lett. 20:5811. |
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