title stringlengths 0 901 | abstract stringlengths 3 9.89k | PMID int64 22 25.3M | embedding listlengths 768 768 |
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Isolation of a growth-stimulating agent from human skin fibroblast cultures. | Cell-free supernatants were harvested from cultures of human skin fibroblasts, were applied on to DEAE-cellulose columns, and the first fraction eluted with phosphate-buffered saline contained the growth-stimulating agent. The eluted fraction was then passed through a series of amicon membranes. After passing through P... | 720,491 | [
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Exogenous melatonin and melanophore development in Xenopus. | Xenopus larvae raised from stage 21 in melatonin solution and upon a dark background had fewer head melanophores at stage 48 than control animals not exposed to melatonin. Rearing larvae in melatonin solution seems to mimic rearing larvae on a light background. | 720,493 | [
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Daily change in pineal N-acetyltransferase activity in a diurnal mammal, the ground squirrel. | Pineal N-acetyltransferase (NAT) activity in the ground squirrel, a diurnal mammal, was found to have a daily fluctuation with peak activity during the dark time. This same daily change is found in nocturnal mammals and diurnal birds. NAT may play an important role in keeping track of light and dark cycles. | 720,495 | [
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Changes in mouse liver superoxide dismutase activity and lipid peroxidation during embryonic and postpartum development. | In inbred mice possessing 'high' and 'low' tissue superoxide dismutase (SOD) activity, it was observed that the difference in the SOD activities of the liver homogenates during development attains the maximum characteristic of the strain by about the 150th day. Subsequently, the SOD activity change displays a tendency ... | 720,503 | [
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Kinetics of interferon action. | A kinetic analysis of the action of interferon with different preparations in chick embryo fibroblast cell culture gives additional evidence for interaction of interferon with the cell surface, compatible with the idea that interferon is not taken up by the cells. With certain assumptions the binding constant is in the... | 720,510 | [
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A mechanism of formation of desmosome-like structures between synovial intimal cells. | Desmosomes or desmosome-like structures do not occur between normal synovial cells but such structures do develop between the synovial cells in cases of traumatic arthritis, rheumatoid arthritis and villonodular synovitis. Morphological evidence is presented suggesting that such structures develop as a result of the in... | 720,528 | [
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Effects of exogenous thyroxine on the surface morphology of the developing chick anterior corneal epithelium. | The production of microvilli on the developing chick anterior corneal epithelium is drastically accelerated by the administration of exogenous thyroxine and the effects are seen as early as 2 days after injection. Thiouracil administration slightly retards the production of microvilli though its effects are not so pron... | 720,529 | [
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Effect of vitamin E on post irradiation death in mice. | The 30-day survival after exposure to 800 Rad 60Co gamma radiation has been compared for female mice maintained on vitamin E deficient, vitamin E supplemented or regular lab rations before and/or after irradiation. Pre- or post-irradiation dietary supplementation had no effect on survival; however, injection of alpha-t... | 720,531 | [
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[Pharmacologic characteristics of triftazin syrup]. | The neurotropic activity, toxicity and pharmacokinetics of triftazine in the form of syrup and powder were subjected to a comparative study on albino mice, rats and dogs. Investigations demonstrated that as follows from the tests under study these drugs display an egual depressing activity. Triftazine from the syrup wa... | 720,579 | [
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A possible mechanism for the detrimental effect of varicocele on testicular function in man. | Seminiferous epithelium histology, Leydig cell density, and in vitro testosterone synthesis were quantitated in bilateral testicular biopsies from men with varying degrees of unilateral or bilateral varicoceles. Results were correlated with plasma levels of gonadotropins (follicle-stimulating hormone [FSH], luteinizing... | 720,647 | [
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[Organization of the electrical activity of different groups of muscles in rats during locomotion]. | The EMG was recorded from the knee synergical group of muscle in the phase of leg move during locomotion in rats. The correlation analysis of the EMG epochs and the pauses in muscular activity respective to the moments of onset and termination of the leg move phase divided it into three independent elements: rise of th... | 720,672 | [
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Trasidrex (a fixed combination of slow Trasicor 16o mg and Navidrex 0.25mg) in the treatment of hypertension: a multicentre clinical trial in general practice. | Sixty-two patients with hypertension who were treated with a free combination of Slow Trasicor or Trasicor and Navidrex K were transferred to a fixed combination tablet, Trasidrex (slow oxprenolol 160 mg + cyclopenthiazide 0.25 mg). Blood pressure control was marginally improved and there was no increase in side-effec... | 720,740 | [
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Effect of thyroidectomy on intracellular amount and distribution of exchangeable Ca2+ in isolated rat liver cells. | The effect of thyroidectomy and replacement therapy on intracellular calcium was studied in isolated rat liver cells. The cells were first loaded with 45Ca and the efflux of isotope followed for 4 h. The cells maintained their viability during this period as judged from staining with trypan blue and rate of respiration... | 720,746 | [
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Diabetic pregnancy: management of diabetes on the day of delivery. | An insulin regimen for management of the insulin dependent diabetic on the day of delivery is described. Pregnant diabetics were fasted on the day of delivery and infused with 10g glucose per hour throughout labour and after delivery until normal eating was resumed. In the morning a fixed dose of intermediate acting in... | 720,777 | [
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[Single-strand DNA breaks and chromosome aberrations in the hepatocytes of mice of different ages]. | The same damages were found to occur in hepatocyte DNA from old mice of different strains by means of sedimentation in alkaline sucrose concentration gradient. At the same time, the number of hepatocytes with aberrant chromosomes is strongly different in these strains. From these data an assumption is made that some ot... | 720,827 | [
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Effect of temperature on short-term preservation of platelets for in vitro tests. | We have evaluated the effect on the response of citrated platelet-rich plasma to aggregating agents of storage at 4 degrees C versus room temperature (21 degrees C) for 2 and 4 h after venipuncture. While there were small decreases in some responses to epinephrine, ADP and collagen attributable to 21 degrees C storage,... | 720,958 | [
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[His bundle electrography in children (author's transl)]. | The His bundle electrography has confirmed many earlier hypotheses about the localization of atrioventricular block and the mechanism of tachyarrythmias; beyond this, it has added new information. In this paper, normal values and results of the method during childhood are reviewed. The indication for His bundle electro... | 721,038 | [
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[Transposition of the great arteries with horizontal interventricular septum (author's transl)]. | Transposition of the great arteries with horizontal interventricular septum is characterized by a superior-inferior relationship of the ventricular chambers in the presence of ventriculo-arterial discordance. Embryologically this very peculiar anatomy can be explained by a rotation of the bulboventricular loop around a... | 721,041 | [
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[Current status of pacemaker technology (author's transl)]. | Since the first cardiac pacemaker was implanted twenty years ago, advances rendered have provided patients with increased reliability and therapeutic efficiency as well as subsequent incrementation in their life expectancy. Technological developments have yielded improvement in the main components such as electrode, th... | 721,047 | [
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[Incidence, natural history and complications of chronic arterial hypertension (author's transl)]. | Representative studies in Switzerland and the Federal Republic of Germany show that 12 to 25% of the adult population have elevated blood pressure values of 160/95 mm Hg or above. The incidence of hypertension increases with age. Approximately 2 to 6% of high school students have hypertension whereas 30 to 50% of perso... | 721,051 | [
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[Pathogenetic, pathophysiological and biochemical aspects of essential hypertension (author's transl)]. | Hereditary and environmental factors are involved in the pathogenesis of essential hypertension. Obesity, salt intake and stress are predominant among the environmental influences. Autonomous nervous dysfunction, increased contractility of vascular smooth muscle cells and impaired renal handling of sodium are major abn... | 721,054 | [
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The genome of Calliphora. Analysis by DNA reassociation kinetics. | The size and nucleotide sequence complexity of the Calliphora vicina genome has been determined from the kinetics of reassociation of its DNA. Unique sequence DNA comprises 80% of the genome and the remainder is divided into medium (14%) and highly repetitive sequences (6%), which are repeated roughly 150 times and mor... | 721,071 | [
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Regulation of the synthesis of human chorionic gonadotropin by strains of HeLa cells in culture. | Thirty-seven strains of HeLa cells were examined for their ability to synthesize human chorionic gonadotropin (hCG) and its alpha subunit (hCG-alpha) in culture. Synthesis of hCG-alpha and hCG also was investigated in the presence of sodium butyrate and 5-bromo-2'-deoxyuridine (BrdUrd). All HeLa strains synthetized hCG... | 721,101 | [
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Studies on the control of antibody synthesis. XII. Genetic influences on antibody affinity. | Genetic controls on the concentration and affinity of the anti-dinitrophenyl antibodies produced by different inbred strains of mice in response to immunization with dinitrophenylated bovine gamma globulin in Freund's complete adjuvant were demonstrated. Based upon data on F1 hybrids and backcrosses, it can be conclude... | 721,135 | [
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Effect of acetazolamide on insulin sensitivity in dogs with alloxan diabetes. | The effect of acetazolamide on the sensitivity to exogenous insulin in the alloxanised diabetic dogs was studied. The administration of acetazolamide caused acidosis and insulin resistance. The liver insulinase activity of diabetic dogs after acetazolamide administration was also studied to evaluate the role of this en... | 721,253 | [
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Effectiveness of N-N-bis-P-chlorophenyl-3p-tolyl glutaric acid diamide (SRC-3605) as a hypocholesterolaemic compound in hypercholesterolaemic female weanling and adult rats. | SRC-3605, N-N-bis-P-chlorophenyl 3-p-tolyl glutaric acid diamide, was studied for its hypocholesterolaemic effect on serum and liver cholesterol in hypercholesterolaemic weanling and adult female rats. Weanlings were administered doses of SRC-3605 ranging from 100 to 300 mg/kg body weight for 4 or 8 consecutive days. T... | 721,249 | [
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Induction of a tumor with greatly increased metastatic growth potential by injection of cells from a low-metastatic H-2 heterozygous tumor cell line into an H-2 incompatible parental strain. | An H-2 heterozygous sarcoma, MDAY, originally induced with methylcholanthrene in an (A X DBA/2)F1 ((H-2a X H-2d) hybrid host was selected for growth in the H-2d homoxygous parental DBA/2 strain by serial intraperitoneal transplantation of ascites tumor cells. An apparent variant, designated MDAY-D2, was obtained which ... | 721,318 | [
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Positive reactions of alcoholic men to sensory deprivation. | Ten male alcoholics who had been abstinent for 2 to 9 weeks volunteered to participate in a study on the effects of sensory deprivation. After 8 hours of lying on a bed in a dark, silent room, they indicated on various measures that they had found the experience to be quite pleasant; in fact, they indicated that they h... | 721,337 | [
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0.3016152,
... |
The effects of alcoholism training on the knowledge, attitudes, and drinking behavior of college students in the human services. | This study reports on the impact of clinically oriented alcoholism training on undergraduate students in the human services preparing for entry level counseling positions. Specifically, this study was designed to determine whether training in alcoholism influences students' knowledge and attitudes toward alcohol and al... | 721,340 | [
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Methadone maintenance: an assessment of potential fluctuations in behavior between doses. | Thirty methadone maintenance outpatients were measured 1 and 25 hours after ingestion of methadone on auditory threshold, distance perception, simple and differential reaction time, time perception, attention span, and short-term memory. The results revealed a small but statistically significant difference between the ... | 721,344 | [
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Screening of the thyroid function of newborns in Southern Belgium. | Data on T4 and TSH values derived from a voluntary screening programme of thyroid function in Belgium are presented. Significant differences in T4 values according to sex, maturity and birthplace are shown. | 721,360 | [
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Observed responses of medical students in a sex education seminar on obstetrics and gynecology. | This article describes the typology of negative responses in a medical student sex education seminar. Seventy medical students were observed during participation in sex education seminars during a seven month period. Their behavior in class was generally appropriate. Negative responses sometimes did occur, however. The... | 721,376 | [
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Studies in family-oriented crisis intervention with hemodialysis patients. | This article describes a family-oriented crisis intervention approach to help patients with chronic renal failure adjust to the unique demands of home dialysis. In particular, home dialysis necessitates a working patient-dialysis partner relationship that has very adaptive problem solving skills. A couple whose permorb... | 721,377 | [
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The oscillatory potentials of the mudpuppy retina. | The properties of the oscillatory potentials (OPs) of the mudpuppy electroretinogram (ERG) were studied and compared with the properties of the b-wave of the ERG and the proximal negative response (PNR). Both transretinal and intraretinal ERGs were recorded in response to full-field as well as 200 and 500 microgram spo... | 721,390 | [
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... |
Vitreous fluorophotometry evaluation of xenon photocoagulation. | An abnormal increase in the permeability of the outer blood-retinal barrier was induced in the eyes of adult pigmented rabbits after retinal xenon arc photocoagulation. The alteration of the blood-retinal barrier, which was assessed by vitreous fluorophotometry after systemic administration of sodium fluorescein, follo... | 721,394 | [
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[In-vitro stimulation of sperm motility using a deproteinized hemodevivative and a luteinizing hormone]. | The effect of a commercial deproteinized blood extract--on motility of human spermatozoa was examined in vitro. The improvement of motility as seen after addition of 5 and 10 percent of this deproteinized blood extract, is supposed to be due to amounts containing different concentrations of LH. The extract was ruled ou... | 721,473 | [
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[Scintigraphy in the diagnosis of osteitis]. | The report on the experience with skeletal scintigraphy in case of osteitis discusses the clinical use of this method. It is shown that with correct indication scintigraphy can furnish early diagnosis and in many cases additional valuable information. | 721,600 | [
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[Plate fixation and open wound treatment in infected intramedullar nails]. | In treating patients who present with an extensive non-union due to infected intramedullary nail, bone healing can be achieved within a few weeks through a policy of active management. Following excision of the infected tissue and thorough irrigation, the fracture site is stabilized by a plate fixation and a packet wit... | 721,602 | [
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[Examination of patients with normal and abnormal voice by pneumotachography (author's transl)]. | 22 patients with normal vocal function and 33 with voice disorders were examined by pneumotachography. The degree of air-flow speed during phonation, the volume of air during phonation, and the duration of phonation were studied in 17 dysphonic patients with complete closure of the glottis, 16 patients with incomplete ... | 721,638 | [
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[Investigations on the incidence of partial agnosia of sounds (author's transl)]. | Stammering as a frequent speech disorder in infancy results from many causes. For instance, it is possible that a child with normal peripheral hearing may be unable to distinguish sounds by tone and therefore will repeat them incorrectly. A testing method is presented in which paired pictures are used which differ by t... | 721,639 | [
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Burst width tracking: brief tone thresholds in the normal ear. | This study presents a unique procedure for determining the critical duration and temporal summation function. With this procedure, duration as a parameter was used to determine the temporal summation characteristics of ten normal ears. An apparatus was constructed that permitted subjects to track threshold by modulatin... | 721,668 | [
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Monitoring of hearing aids and earmolds in an educational setting. | Classroom hearing aid and earmold inspection of the amplification worn by 109 school-age children was performed by an audiologist. Hearing aids and earmolds were classified as adequate or inadequate depending on whether optimal function of the hearing aids and good fit of the earmolds was found. Over 60% of the childre... | 721,671 | [
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Use of cytology for tumor diagnosis in private veterinary practice. | Cytologic and histologic services were offered without charge to veterinarians in the greater Tulsa area by the Tulsa Registry of Canine and Feline Neoplasms. During the first 3 years of operation, all veterinarians treating dogs or cats used the histologic service and slightly less than one-half used the cytologic ser... | 721,675 | [
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Mitotic rate of the rat's thyroid gland during hypertrophy induced by an antithyroid agent (carbimazole). | Female rats (200 g body weight) were either untreated or given carbimazole (0.1 g/100 ml) in their drinking water for up to 12 weeks. The mitotic rate of the thyroid follicular cells was estimated using vincristine sulphate. Measurement of whole-body oxygen consumption showed that the rats were clearly hypothyroid afte... | 721,696 | [
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Isolation and characterization of elasnin, a new human granulocyte elastase inhibitor produced by a strain of Streptomyces. | Elasnin, a new human granulocyte elastase inhibitor, produced by the strain of KM-2753 designated as Streptomyces noboritoensis KM--2753 has been isolated from the fermentation broth by column chromatography on silica gel and neutral alumina. Elasnin is a neutral, colorless, and viscous oil (ND17 = 1.4983, [alpha]18D -... | 721,707 | [
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High performance liquid chromatography of natural products. II. Direct biological correlation of components in the fermentation broth. | A portion of the eluent, during liquid chromatography of a fermentation broth, is applied directly to a paper strip at the same rate as the recording of the U. V. absorption. The paper is then placed on the agar inoculated with an appropriate test organism. This procedure permits direct correlation of biological activi... | 721,709 | [
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Metabolism of trichothecene mycotoxins. II. Substrate specificity of microsomal deacetylation of trichothecenes. | The substrate specificity of microsomal nonspecific carboxyesterase [EC 3.1.1.1] from rabbit and rat livers was studied in vitro by using seven (A)-type and six (B)-type 12,13-epoxytrichothecene mycotoxins. The C-4 acetyl residues of diacetoxyscirpenol, T-2 toxin, monoacetylnivalenol (fusarenon-X), and diacetylnivaleno... | 721,800 | [
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Photochemical cross-linking of reovirus genome RNA in situ and inactivation of viral transcriptase. | Reovirus infectivity and core-associated RNA polymerase activity were decreased by irradiation with long wavelength ultraviolet light in the presence of the 4'-substituted psoralen derivatives, 4'-aminomethyl-4,5',8-trimethylpsoralen and 4'-hydroxymethyl-4,5',8-trimethylpsoralen. Monoadduct formation occurred after pho... | 721,805 | [
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Cysteine conjugate beta-lyase in rat liver. A novel enzyme catalyzing formation of thiol-containing metabolites of drugs. | A novel enzyme catalyzing cleavage of the thioether linkage in cysteine conjugates of aromatic compounds, such as 2,4-dinitrobenzene and p-bromobenzene, has been purified about 500-fold from rat liver cytosol. Incubation of S-(2,4-dinitrophenyl)cysteine with the enzyme preparation yielded 2,4-dinitrobenzenethiol, pyruv... | 721,818 | [
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Synergistic regulation of phosphorylase a by glucose and caffeine. | Kinetic studies of both liver and muscle phosphorylase a demonstrate that caffeine and glucose inhibit the binding of glucose 1-phosphate to the enzyme in a synergistic competitive and nonexclusive manner. Inhibition studies for numerous other caffeine analogs show that the muscle enzyme has a relaxed specificity for t... | 721,834 | [
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Anterior subluxation of the lateral tibial plateau. A diagnostic test and operative repair. | Recurrent anterior subluxation of the lateral tibial plateau is a common type of chronic knee instability resulting from trauma. It can be reproduced by the clinical test described and corrected by a surgical procedure called the sling and reef operation, in which a strip of iliotibial tract is used to create a sling a... | 721,850 | [
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Acute compartment syndromes: diagnosis and treatment with the aid of the wick catheter. | Intracompartmental pressures were measured by the wick catheter technique in sixty-five compartments of twenty-seven patients who were clinically suspected of having acute compartment syndromes. A pressure of thirty millimeters of mercury or more was used as an indication for decompressive fasciotomy. The range of norm... | 721,856 | [
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... |
Retroperitoneal abscess. A potentially fatal complication of closed fracture of the pelvis. | Two patients were seen in whom a retroperitoneal abscess developed following closed pelvic fracture. A retroperitoneal abscess is often unsuspected clinically, and presents a difficult diagnostic challenge. Prompt recognition and treatment are essential to patient survival. | 721,865 | [
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The radiologist's opportunity to teach bone dynamics. | Activity and muscle strength are important for bone growth and for development and maintenance of bone density. The rediologist should help students to understand the important relationship between muscular activity and bone strength. | 721,881 | [
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Reflux of barium into a maxillary antrum. | A patient with achalasia and neurologic deficit permitting intermittent nasopharyngeal reflux developed unilateral maxillary sinusitis. Heavy metal density in the center of the inflamed sinus almost certainly was barium sulfate refluxed during an upper gastrointestinal study. | 721,888 | [
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Heterogeneity in sensitivity to cleavage by the restriction endonucleases ECORI and HindIII of circular kinetoplast DNA molecules of Crithidia acanthocephali. | Kinetoplast DNA (kDNA) of the protozoan Crithidia acanthocephali consists mainly of an association of approximately 27,000 covalently closed, 0.8-micron (1.58 X 10(6) daltons) circular molecules apparently held together in a particular structural configuration by topological interlocking. The sensitivities of circular ... | 721,893 | [
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... |
Improved method for the rapid determination of isosorbide dinitrate in human plasma and its application in pharmacokinetic studies. | A rapid, accurate and highly sensitive method was developed for the determination of isosorbide dinitrate in human plasma. Concentrations in the lower nanogram and subnanogram range are determined by a one-step extraction of 2 ml plasma, containing 4 ng/ml nitroglycerine as internal standard, with 5.5 ml n-pentane. The... | 721,922 | [
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Techniques for detecting enzymes in high-performance liquid chromatography. | Techniques are described for the automated detection of a series of enzymes in a high-performance liquid chromatographic system. Detection was achieved by either a direct or a coupled enzyme assay using photometric detectors. In direct detection the immediate enzymatic product was monitored. Coupled enzyme assays requi... | 721,937 | [
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Study of water treatment effects on organic volatiles in drinking water. | Volatile organics were determined during all stages of the water treatment process in a municipal water works. Analytical parameters for a quantitative procedure were investigated. The method used allows simultaneous determination of both halogenated and non-halogenated purgeable organics. Approximately 70 substances w... | 721,940 | [
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Thermotolerance of Wangiella dermatitidis. | A variety of diagnostic tests used by many laboratories to identify isolates of Wangiella dermatitidis (= Fonsecaea dermatitidis) were evaluated. Thirteeen isolates of W. dermatitidis were studied with respect to their ability to grow at 25, 37, 40, 45, and 50 degrees C, colonial and micromorphology, gelatin liquefacti... | 721,946 | [
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Some features of the spatial organization of the central nucleus of the inferior colliculus of the cat. | A series of neuroanatomical and neurophysiological experiments have been conducted within the central nucleus of the inferior colliculus (ICC) of the cat in order to determine some features of the spatial organization of the nucleus. Results from these experiments have demonstrated: (1) the origins of the auditory brai... | 721,973 | [
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Proliferative and degenerative events in the early development of chick dorsal root ganglia. I. Normal development. | Development of the chick dorsal root ganglia was examined in 4.5- to 9.5-day embryos. Tritiated thymidine (3H-TdR) and autoradiography was used to analyze proliferative activity and the Feulgen procedure to analyze degenerative activity in ganglia 12-17. Proliferative activity was found to be elevated through 4.5 days ... | 721,975 | [
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Menu item forecasting systems in hospital foodservice. A cost comparison of two- and three-echelon systems. | The forecasting efficiency of two- and three-echelon menu item forecasting systems was compared. Two forecasting models were used with each system, adaptive exponential smoothing and a Box-Jenkins model. The two systems were compared in terms of forecast error costs. The two-echelon system, using adaptive exponential s... | 722,013 | [
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A complete dislocation of the thumb metacarpal. | Multiple dislocations of a single finger are rare. We report a patient with complete dislocation of the thumb metacarpal, a condition not reported previously. A 9 year follow-up shows the patient to have a normal-appearing, strong, and painless hand. | 722,029 | [
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Experimental infection of Ancylostoma caninum in mice. III. distribution of larvae after repeated exposures to infection. | Different groups of mice were infected with various single or repeated doses of larvae of Ancylostoma caninum. Considerable loss of larvae occurred in the immunised groups when compared with their counterpart unimmunised groups. Larval migration and distribution in the different tissues was less in immunised mice, as m... | 722,039 | [
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Simultaneous localization of an hepatic binding protein specific for galactose and of galactose-containing receptors on rat hepatocytes. | The hepatic binding protein, specific for galactose-terminated glycoproteins (asialoglycoproteins) and the receptors for the Ricinus communis lectin, specific for galactose residues (RCA1), were simultaneously localized on isolated rat hepatocytes by the gold method. The marker for the binding protein was prepared from... | 722,052 | [
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Physician-induced demand for medical care. | This paper addresses the theoretical models designed to ascertain the existence of a variable level of physicians' activity in shifting the demand of their patients. Two basic approaches are followed: equilibrium models of the demand for health care, and disequilibrium models. Within the former category, both competiti... | 722,067 | [
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On the rationing of health services and resource availability. | Government controls on availability of physicians and hospital resources can counteract some undesired aggregate consequences of reimbursement insurance. Given aggregate controls, many important questions arise concerning nonprice rationing of services by physicians. A theoretical model of rationing should be consisten... | 722,071 | [
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beta-Estradiol reduces natural killer cells in mice. | beta-estradiol was administered to mice continuously by diffusion from a silastic tube that was implanted subcutaneously at 4 weeks of age. Four to 6 weeks of estrogen administration caused a substantial reduction in natural killer cell activity in the spleens from mice of either sex. Androgen (5alpha-dihydrotestostero... | 722,074 | [
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Induction of lymphocyte colony formation in vitro by protein A. | Human lymphocyte colonies were grown in semi-solid culture in the presence of staphylococcal protein A. The best results were obtained when 1 X 10(6) lymphocytes were cultured for 5 to 7 days with 25 to 50 microgram of protein A per dish. A lineal relationship was observed between the number of cells seeded and the num... | 722,077 | [
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The binding of activated C3 to polysaccharides and immunoglobulins. | The nature of the acceptors for activated C3 present on immunoglobulins was studied by using the fixation of C3 to Sepharose beads after activation with trypsin as a model system. C3 fixation to Sepharose is a property exclusively linked to the short active state of C3. This binding could be inhibited by various carboh... | 722,084 | [
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Rapid lymphocyte immunoreactivity test utilizing [3H]uridine in vitro. | A microculture assay utilizing [3H]uridine incorporation was developed to test murine spleen lymphocyte immunoreactivity in vitro. Parameters of the culture technique which included cell density, doses of LPS, Con A, PHA, [3H]uridine levels, and length of culture time were investigated. Responses were detectable at 4 h... | 722,088 | [
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A micro immunoassay method for measuring IgG antibodies using staphylococcal protein-A. | A micro radioimmunoassay procedure has been developed for assaying IgG antibodies to ragweed and grass allergen components. The method offers a rapid and reproducible procedure for estimating antibodies in human sera and is well adapted for monitoring immunological responses following hyposensitisation therapy. | 722,089 | [
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The determination of lymphoid cell chimerism using peripheral blood lymphocytes from murine bone marrow chimeras. | A simple, rapid and accurate method was devised for deteriming lymphoid cell chimerism in bone marrow-reconstituted mice. Chimeras were produced by reconstituting lethally irradiated mice with semi-allogeneic bone marrow cells. Lymphocytes from the peripheral blood of individual chimeric mice were purified by sedimenta... | 722,099 | [
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Mechanisms of carcinogenesis: dose response. | There is great controversy whether the carcinogenicity of chemicals is dose-dependent and whether a "threshold" dose exists below which cancer will not be induced by exposure. Evidence for dose-dependency exists and is believed to be accepted generally if extricated as it should be from the "threshold" concept. The "th... | 722,184 | [
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Hormonal carcinogenesis: a novel hypothesis for the role of hormones. | A testable hypothesis on the role of hormones in carcinogenic processes is presented. The hypothesis has been based on the findings that (1) hormones regulate cell division in normal cells, (2) successful neoplastic transformation requires hormonal stimulation of cells receiving carcinogenic insult, (3) normal cells ha... | 722,216 | [
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Liquid-tissue behavior and differential cohesiveness during chick limb budding. | Emerging chick limb-buds at first grow only in length, not width. The growth parameters of limb mesoderm--cell shapes, distributions, division patterns and cleavage orientations--are incompatible with representations of this tissue as an elongating solid composed of proliferating but immobile cells. We observe that sam... | 722,227 | [
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Participation of neural crest-derived cells in the genesis of the skull in birds. | The differentiation of cephalic neural crest cells into skeletal tissue in birds has been observed using the quail-chick nuclear marking system, which is based on specific differences in the distribution of the nuclear DNA. Chimaeras were formed by replacing a fragment of cephalic neural primordium of a 2- to 12-somite... | 722,230 | [
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Role of phagocytosis in the activation of macrophages. | Macrophages were obtained by peritoneal lavage from untreated mice or from mice which had received either Brewer's thioglycollate broth or a suspension of streptococcus A cell walls intraperitoneally 4 days before. 3 h after harvesting, adherent cells from untreated mice were allowed to phagocytose zymosan, formaldehyd... | 722,242 | [
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Isolation and characterization of polysheaths, phage tail-like defective bacteriophages of Alcaligenes eutrophus H 16. | Polysheaths were spontaneously formed inside cells of the hydrogen bacterium Alcaligenes eutrophus H 16. These particles are long tube-like structures of 24 nm diam. belonging to the phage tail-like defective bacteriophages (Lotz, 1976). In mid log-phase fermenter-grown cells, polysheaths were observed in about 20% of ... | 722,280 | [
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Paranoid delusions and auditory hallucinations associated with digoxin intoxication. | The 83-year-old woman in this case report developed paranoid delusions and auditory hallucinations in association with toxic serum levels of digoxin, while remaining alert, unagitated, and coherent in thinking. No cardiovascular or metabolic abnormalities were discovered to account for her psychiatric symptoms. Her men... | 722,304 | [
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The diagnosis of antisocial and hysterical personality disorders. An example of sex bias. | The author submits evidence to support the contention that antisocial and hysterical personality disorders are essentially sex-typed forms of a single condition. The features of each condition, he argues, are either common to both disorders or are related to sexual stereotypic traits. He reports on a study which indica... | 722,306 | [
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Sequential myocardial scintigraphy with technetium-99m stannous pyrophosphate following myocardial infarction. | Studies have shown that technetium-99m stannous pyrophosphate (Tc-PPi) is effective for the detection and imaging of acute myocardial infarction. Positive Tc-PPi myocardial scintigrams, however, have been reported in patients with other forms of heart disease and no evidence of recent myocardial infarction. To help def... | 722,321 | [
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Radionuclide kymography for the assessment of regional myocardial wall motion. | Regional myocardial wall motion is usually evaluated qualitatively from ECG-gated end-systolic and end-diastolic blood-pool images. Radionuclide kymography, which displays a one-dimensional scintigraphic image in time, synchronized with the electrocardiogram, provides a method to quantitate this motion. The technique ... | 722,323 | [
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0.00852974,
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0.5670704,
0.03058825,
0.09526112,
... |
Disappearance of a hyperfunctioning thyroid nodule following TSH stimulation. | Following stimulation with exogenous TSH, a patient with a large hyperfunctioning autonomous thyroid nodule developed transient hyperthyroidism and decreased radioiodine uptake, and subsequently the nodule disappeared. | 722,327 | [
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0.007783033,
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0.1628572,
0.08811881,
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Ultrastructural observations on epithelioid cell granulomas induced by zirconium in the guinea-pig. | An ultrastructural study has been made of the allergic-type nodular granulomas that can be induced in guinea-pigs sensitised to sodium zirconium lactate. Lesions taken at the peak of granuloma development 7 days after skin testing contained epithelioid cells which could be recognised particularly by their content of ro... | 722,372 | [
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... |
The life cycle of Eimeria dispersa Tyzzer 1929 from the turkey in gallinaceous birds. | The life cycle of a turkey strain of Eimeria dispersa was studied in chickens, Chukar partridge, Ring-necked pheasant, and Bobwhite quail. Endogenous development in partridge and quail differed from development in chickens and pheasant. In partridge and quail, mature 1st-, 2nd-, and 3rd-generation schizonts, macrogamet... | 722,458 | [
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0.54658,
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Arousal as a necessary condition for attitude change following induced compliance. | This study examined whether arousal is or is not a necessary condition for attitude change in the induced compliance paradigm. In a 2 times 3 design, experimental subjects were induced to write counterattitudinal essays under either high- or low-choice conditions. All subjects were led to believe that a pill, which the... | 722,473 | [
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... |
ESTRIP, a BASIC computer program for obtaining initial polyexponential parameter estimates. | A new BASIC exponential stripping program, ESTRIP, allows the relatively rapid calculation of initial polyexponential parameter estimates, as does the previously published FORTRAN IV program, CSTRIP. The potential advantages of the new program are that it can be run on microcomputers and minicomputers with BASIC capabi... | 722,481 | [
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0.049936... |
New microporous cholestyramine analog for treatment of hypercholesterolemia. | A new, microporous, uniformly reticulated preparation of cholestyramine is described. The preparation, cholpor, has a higher exchange capacity for chloride than does cholestyramine and swells very little in water. It is 15--20% more potent than chloestyramine in the in vitro binding of sodium cholate; moreover, the bin... | 722,483 | [
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0.1536169,
... |
Kinetic and thermodynamic aspects of in vitro interphase transfer of tetracyclines I: influence of hydroxyl group substitution. | The influence of hydroxyl group substitution on the kinetic and thermodynamic aspects of the interphase transfer of three tetracycline derivatives was studied in a two-phase in vitro system composed of an aqueous pH 5.5 buffer and 1-octanol. Data are reported on the kinetic and thermodynamic parameters of activation, t... | 722,486 | [
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0.2868706,
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... |
Quaternary acetate and propionate esters of 3-hydroxyquinolizidine. | The preparation of the quaternary acetate and propionate esters of 3-hydroxyquinolizidine is described. Tentative structures are assigned on the basis of NMR data. Results of preliminary pharmacological screening of the methiodide and hydrochloride salts are given. | 722,499 | [
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0.0491278,
0.14437... |
Measurements of muscle stiffness and the mechanism of elastic storage of energy in hopping kangaroos. | 1. A kangaroo hopping above a certain speed appears to consume less oxygen than a quadrupedal mammal, of similar weight, running at the same speed (Dawson & Taylor, 1973). This is thought to be achieved by storage of elastic energy in tendons and ligaments. 2. Energy can be stored in a tendon by stretching it, but ... | 722,527 | [
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0.07581606,
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Inhibition of noradrenaline release by adenosine. | 1. Release of [3H]noradrenaline evoked by stimulation (5 Hz) of the pre-labelled rat vas deferens was reduced to 50% by adenosine (10(-6) g/ml.). Inhibition of release was dependent on the concentration of adenosine and was inversely related to the frequency of stimulation. Phenoxybenzamine did not interfere with the a... | 722,535 | [
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Efflux of inorganic phosphate from mammalian non-myelinated nerve fibres. | 1. Uptake and release of radiophosphate were measured in desheathed rabbit vagus nerve. 2. During incubation in Locke containing 0.2 mM-[32P]phosphate a slow labelling of water soluble compounds of the nerve was found; the labelling of the non water soluble compounds was much smaller. During washing with inactive Locke... | 722,557 | [
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0.09825496,
... |
Gastrins in cat and dog: evidence for a biosynthetic relationship between the large molecular forms of gastrin and heptadecapeptide gastrin. | 1. Extracts of antral, duodenal and jejunal mucosa contained the same concentrations and molecular forms of gastrin in cat and dog. In both species component III constituted 93%, component II 4% and components I and IV each 1% of the total immunoreactivity. 2. During electrical vagal stimulation or feeding in (a) anaes... | 722,581 | [
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Involvement of uptake1 and uptake2 in terminating the cardiovascular activity of noradrenaline in normotensive and genetically hypertensive rats. | 1. In pithed, pancuronium-treated rats, inhibition of Uptake1 with desmethylimipramine (2.5 mg/kg i.v.) increased the time course of the pressor response to vasomotor nerve stimulation (1 and 5 Hz for 5 sec) but not the time course of the pressor response to injected noradrenaline (10 and 50 ng i.v.). 2. Subsequent inh... | 722,583 | [
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0.09435958,
0.3627554,
0.5518182,
-0.05056321... |
Simultaneous measurement of pressure in the interstitium and the terminal lymphatics of the cat mesentery. | 1. Simultaneous measurements of the pressure in terminal lymphatics and interstitial tissue have been made in the exteriorized cat mesentery superfused with either physiological salt solution (Krebs solution) or a water-immiscible fluorocarbon, FC-80. 2. The pressures within individual terminal lymphatics were measured... | 722,586 | [
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... |
Relaxation of ventricular cardiac muscle. | 1. The load bearing capacity during relaxation of ventricular cardiac muscle from various animal species was investigated. 2. The effect of load on the time course of relaxation was analysed either by comparing afterloaded contractions against various loads or by imposing abrupt alterations in load (load clamps). 3. In... | 722,587 | [
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Developing role of sensorimotor cortex and pyramidal tract neurons in contact placing in kittens. | Unilateral or bilateral removal of kitten sensorimotor (SM) cortex prior to the fourth postnatal week was followed by recovery of contact placing (CP) to stimulation of any of the four forepaw cutaneous fields. However, immediately after a removal under ethyl chloride, CP was depressed contralaterally, especially to la... | 722,594 | [
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0.210276,
-0.... |
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