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} | ['gnd:4690080-9', 'gnd:4138452-0', 'gnd:4172260-7'] | ['Schiffspropeller', 'Oberflächenströmung', 'Numerische Strömungssimulation'] | ['phy'] | [['gnd:140779922', 'gnd:140779663']] | [['(firstid)GBV:835349276', '(ppn)835349276']] | ['Techn. Univ.'] | [['(classificationName=bk, id=106413783)50.33 - Technische Strömungsmechanik', '(classificationName=ddc)620: Ingenieurwissenschaften', '(classificationName=ddc-dbn)620', '(classificationName=linseach:mapping)ver', '(classificationName=linseach:mapping)phy', '(classificationName=bk, id=106420577)55.40 - Schiffstechnik, Schiffbau']] | ['Non-viscous calculation of propeller forces under consideration of free surface effects'] | [['This thesis reports on the development of numerical methods for the determination of propeller loads for propellers operating under free water surface influences and due to ventilation. The numerical methods are potential-based and different approaches with a boundary element method and a solver coupling approach between a boundary element method and a RANSE solver are developed. The boundary conditions at the free water surface are implemented in the boundary element method in a steady and an unsteady formulation and a validation is given for computed results against experimental data. Unsteady forces and moments are computed for propellers with shallow immersion, propellers with shallow immersion in waves and for ventilating propellers. The results of the numerical methods are compared to experimental results.$cen$dAbstract', 'In dieser Arbeit werden numerische Methoden entwickelt, mit denen die dynamischen Wechsellasten, die auf Schiffspropeller unter Einfluss der freien Wasseroberfläche oder unter Ventilationseinfluss wirken, berechnet werden können. Die numerischen Methoden sind potentialbasiert, eine Randelementmethode und eine Kopplungsmethode zwischen einer Randelementmethode und einem RANSE Löser werden entwickelt. Die Randbedingungen an der freien Wasseroberfläche werden in einer stationären und einer instationären Formulierung in die Randelementmethode implementiert und die Ergebnisse werden mit Messdaten validiert. Die instationären Kräfte und Momente, welche für Propeller berechnet werden, die gering getaucht sind, oder Propellern, die gering getaucht sind und im Einfluss von Wellen operieren, werden Messdaten gegenübergestellt.$cde$dAbstract']] | ['2015'] | ['http://id.loc.gov/vocabulary/iso639-1/en'] | ['open access'] | [[{'@id': 'gnd:4690080-9'}, {'@id': 'gnd:4138452-0'}, {'@id': 'gnd:4172260-7'}]] | ['gnd:10194305-2', 'gnd:1120050685', 'gnd:140779663', 'gnd:140779922', 'gnd:4138452-0', 'gnd:4172260-7', 'gnd:4690080-9', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A835349276'] | ['Technische Universität Hamburg-Harburg, Institut für Fluiddynamik und Schiffstheorie', 'Greve, Martin', 'Krüger, Stefan', 'Abdel-Maksoud, Moustafa', 'Schiffspropeller', 'Oberflächenströmung', 'Numerische Strömungssimulation'] | 22 |
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} | ['gnd:4041418-8', 'gnd:4037882-2', 'gnd:4809855-3'] | ['Massenspektrometrie', 'Naturstoff', 'Prenylgruppe'] | ['che'] | ['Technische Informationsbibliothek (TIB)'] | [['(doi)10.25673/1562', '(firstid)GBV:837435544', '(ppn)837435544']] | ['Universitäts- und Landesbibliothek Sachsen-Anhalt'] | [['(classificationName=linseach:mapping)bio', '(classificationName=ddc-dbn)540', '(classificationName=linseach:mapping)che', '(classificationName=bk, id=181571129)35.76 - Aminosäuren, Peptide, Eiweiße', '(classificationName=bk, id=106407856)35.50 - Organische Chemie: Allgemeines']] | ['Mass spectrometry, biological screening and informatics of prenylated natural products : [kumulative Dissertation]'] | [['prenylatednatural product; electrospray tandem mass spectrometry;fragmentation pattern; Basidiomycetes; Suillus; Albatrellus; antibacterial activity; Activity-correlation-Analysis; MS-based metabolite profiling', 'For the characterization of prenylated compounds fragmentation studies were performed using both positive and negative ion electrospray ionization mass spectrometry (ESI-MSn). MS-based metabolite profiling und fingerprinting investigations of Suillusspecies and multivariate data analysis show the importance of simplifying and visualizing complex data to answer biological questions like classification of biological samples, the influence of geographical origins, and the identification of potential biomarkers. With the involvement of biological screening methods, informatic approaches such as activity correlation analysis (AcorA) allow the prediction of potentially bioactive-relevant compounds in complex mixtures like crude extracts without prior isolation. It could be shown, that mass spectrometry and detailed fragmentation studies as well as mass spectral data of reference compounds enable the identification of prenylated and nitrogen-containing natural products in biological samples.', 'prenylierte Naturstoffe; Elektrospay-Tandemmassenspektrometrie; Fragmentierungsmuster; Basidiomyzeten; Suillus; Albatrellus; antibakterielle Aktivität; Aktivitäts-Korrelationsanalyse; MS-basierteMetabolitenprofile', 'Zur Charakterisierung prenylierter Verbindungen wurden Fragmentierungsstudien mittels Elektrospray-Tandemmassenspektrometrie (ESI-MSn) im positiven und negativen Ionenmodus durchgeführt. Die MS-basierten Metabolomics-Untersuchungen von Suillus-Extrakten zeigen, dass die Vereinfachung und Visualisierung von komplexen Daten die Klassifizierung von Arten, die Unterscheidung von biologischen Proben und den Einfluss der geographischer Herkunft sowie die Identifizierung potenzieller Biomarker ermöglicht. MS-basierte Metabolitenprofile und Bioaktivitäten aus biologischem Screening können mittels bioinformatischer Ansätze wie der Aktivitätskorrelationsanalyse (AcorA) korreliert werden und erlauben eine Vorhersage aktivitätsrelevanter Metaboliten in komplexen Gemischen ohne vorherige Reinigung und Isolierung. MS-Fragmentierungsstudien und Daten von Referenzsubstanzen dienen als Grundlage für die Identifizierung von prenylierten und stickstoffhaltigen Metaboliten in biologischen Proben.']] | ['2015'] | ['http://id.loc.gov/vocabulary/iso639-1/de'] | ['commercial licence'] | [[{'@id': 'gnd:4041418-8'}, {'@id': 'gnd:4037882-2'}, {'@id': 'gnd:4809855-3'}]] | ['gnd:4037882-2', 'gnd:4041418-8', 'gnd:4809855-3', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A837435544'] | ['Massenspektrometrie', 'Naturstoff', 'Prenylgruppe'] | 5 |
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} | ['gnd:4140566-3', 'gnd:4796905-2', 'gnd:4078889-1'] | ['Verzweigung (Mathematik)', 'Seltsamer Attraktor', 'Fraktale Dimension'] | ['mat'] | ['Technische Informationsbibliothek (TIB)'] | [['(firstid)GBV:837691834', '(ppn)837691834']] | [''] | [['(classificationName=linseach:mapping)mat', '(classificationName=ddc-dbn)510']] | ['Strange attractors of forced one-dimensional systems : existence and geometry'] | ['Non-smooth saddle-node bifurcations of one-parameter families of quasiperiodically driven dynamical systems on the real line give rise to strange non-chaotic attractors. In this thesis, we provide a class of families which has non-empty interior in the C2-topology and whose elements undergo non-smooth saddle-node bifurcations. Within this class, we study the geometry of the corresponding strange attractors by computing different fractal dimensions. In particular, we show that the Hausdorff dimension differs from the box-counting dimension. We further obtain a description of the minimal set at the bifurcation as a maximal invariant set. Our results treat both the discrete and the continuous time case. A number of explicit examples emphasise the applicability of our findings.'] | ['2015'] | ['http://id.loc.gov/vocabulary/iso639-1/en'] | ['open access'] | [[{'@id': 'gnd:4140566-3'}, {'@id': 'gnd:4796905-2'}, {'@id': 'gnd:4078889-1'}]] | ['gnd:1084605473', 'gnd:36164-1', 'gnd:4078889-1', 'gnd:4140566-3', 'gnd:4796905-2', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A837691834'] | ['Fuhrmann, Gabriel', 'Friedrich-Schiller-Universität Jena', 'Verzweigung (Mathematik)', 'Seltsamer Attraktor', 'Fraktale Dimension'] | 16 |
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T-peel tests and electron micrographs revealed four different peel regimes, depending on the parameter combination: (I) Interlaminar peeling at low peel strength with uniform peeling along a weakly bonded PE lamination layer; (II) transition tearing at intermediate peel strength showing areas of interlaminar peeling and translaminar tearing; (III) translaminar tearing at high peel strength showing tears through the entire film; and, (IV) undefined tearing at varying tear strength occurring when vibration effects during welding lead to insufficient contact of the films or high pressures lead to a displacement of PE. ...', 'Zusammenfassung: Botulinum Neurotoxine (BoNT) gehören zu den stärksten bekannten Toxinen, sind relativ einfach herstellbar und wurden bereits in militärischen Waffen integriert. Daher finden sich BoNT in der Kategorie von Toxinen, von denen die größte Bedrohung durch Bioterrorismus ausgeht. Neben der möglichen vorsätzlichen Freisetzung von BoNT treten auch natürliche Ausbrüche in der Tierhaltung und Lebensmittelherstellung auf, in denen Vor-Ort-Analysen benötigt werden. In solchen Szenarien werden schnelle (< 1 Stunde), tragbare und automatisierte Analysen von unbekannten BoNT-Typen und -Formen verlangt. 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Eine in dieser Arbeit durchgeführte Parameterstudie für das Ultraschallschweißen erlaubt die Verpackung von Reagenzien in Aluminium/Polyethylen-Verbundfolie. Die Siegelnähte der verschweißten Verpackung können so ausgelegt werden, dass sie bei einem definierten Druck delaminieren und die Reagenzien freisetzen. Die Siegezeit, der Schweißdruck und die Ultraschall-Amplitude wurden im Bereich 100 – 400 ms, 50 – 250 kPa und 12 – 24 µm variiert und deren Einfluss auf die Siegelstärke untersucht. 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} | ['gnd:4375592-6', 'gnd:4460230-3'] | ['Galliumnitrid', 'Indiumnitrid'] | ['phy'] | ['Technische Informationsbibliothek (TIB)'] | [['(ppn)846259044', '(contract)FRUB-opus-10507', '(firstid)BSZ:454071825', '(doi)10.6094/UNIFR/10507']] | [''] | [['(classificationName=linseach:mapping)phy', '(classificationName=ddc)530', 'Quantenwell', '(classificationName=bk, id=10640444X)33.68 - Oberflächen, Dünne Schichten, Grenzflächen', '(classificationName=bk, id=10641917X)33.72 - Halbleiterphysik', 'Lumineszenzdiode', '(classificationName=ddc-dbn)530', 'Photolumineszenzspektroskopie', 'STED-Mikroskopie']] | ['Charge carrier dynamics in InGaN quantum wells : stimulated emission depletion and lateral charge carrier motion'] | ['Zusammenfassung: Over the last decade Indium Gallium Nitride (InGaN) based LEDs have become one of the dominating light sources in cases where high efficiency and compact form factors are required. Despite having already reached a very high efficiency level, there is still room for further improvement. Charge carrier motion and recombination dynamics are essential processes when it comes to understanding and improving solid state based lighting technology on a fundamental level. Two experimental techniques based on confocal microscopy are used in the present work to study charge carrier dynamics and motion in InGaN based quantum well LED structures. It focuses on the investigation of stimulated emission depletion (STED) and lateral charge carrier motion, i.e. motion of charge carriers within the quantum well plane. In the first part the properties of the STED effect in blue InGaN quantum wells are studied using different continuous wave and pulsed laser sources. The samples are excited at 405 nm and depletion of the excited charge carriers is induced by stimulated emission using a second laser source with a wavelength in the long wavelength part of the fluorescence spectrum. It is found that only a partial fluorescence suppression of about 10% can be achieved in the investigated samples. This is not sufficient to use the STED effect for spatial resolution enhancement like it has already been successfully demonstrated for fluorescence dyes in studies of other authors. Two main processes are identified to be responsible for the partial fluorescence suppression. The first is the availability of only a limited number of localized states in the band structure being susceptible to depletion by stimulated emission. Their number is too small to allow for a sufficient depletion rate. The second effect is strong two photon absorption induced by the depletion laser. The enhanced non-linear absorption leads to an increase in fluorescence intensity and thus counteracts the depletion effect over a wide range of depletion laser powers. Extending the rate equation model normally used to describe STED by two photon absorption shows, that there might still be a chance to achieve higher fluorescence suppression outside of the experimentally accessible parameter space. A confocal time of flight method is used in the second part to measure for the first time the temperature and charge carrier density dependent in-plane diffusion constant of a turquoise InGaN multi quantum well ...'] | ['2015'] | ['http://id.loc.gov/vocabulary/iso639-1/en'] | ['open access'] | [[{'@id': 'gnd:4375592-6'}, {'@id': 'gnd:4460230-3'}]] | ['gnd:1081252294', 'gnd:4375592-6', 'gnd:4460230-3', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A846259044'] | ['Solowan, Hans-Michael', 'Galliumnitrid', 'Indiumnitrid'] | 22 |
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"title": "Mesenchymal stromal cell therapy for dystrophic epidermolysis bullosa",
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"Zusammenfassung: Dystrophic epidermolysis bullosa (DEB) is a rare heritable skin fragility disorder caused by mutations in the COL7A1 gene, coding for the anchoring fibril protein collagen VII. Under physiological conditions the unique function of collagen VII is the mediation of skin stability by attaching the epidermis to the underlying papillary dermis. Reduced amounts or the complete absence of collagen VII in patients with DEB result in severe cutaneous and extra-cutaneous disease manifestations including life-long mechanosensitivity of skin and mucosal surfaces, emergence of large body surface erosions, chronic wounds, secondary fibrosis and development of aggressive squamous cell carcinoma. The consequences of severe DEB massively impede the quality of life from the earliest years on and are hard to bear for the patient and the familiy. Despite tremendous efforts by the scientific community and pharmaceutical companies, no curative therapy is yet available for the treatment of DEB. This study describes the first long-term follow up of locally injected human mesenchymal stromal cells (MSCs) as a therapy option for DEB. Human bone marrow derived MSCs secreted stable triple helical collagen VII in vitro in a comparable amount to human fibroblasts and independent of individual donor age, gender or passage number. Repeated intradermal injections of MSCs in a DEB mouse model resulted in production and deposition of collagen VII at the dermal-epidermal junction, the physiological site of function. The effect was dose dependent with MSCs being up to ten-fold more potent than dermal fibroblasts. MSCs promoted regeneration of DEB wounds via normalization of dermal and epidermal healing and improved skin integrity through de novo formation of anchoring fibril-like structures. Additional benefits were gained by MSCs\u2019 anti-inflammatory effects, which led to decreased immune cell infiltration into injured DEB skin. In this setting the clinical benefit of MSC injections lasted for more than three months.The data from this study suggest that locally applied MSCs are a viable option for DEB therapy, as they provide sufficient amounts of functional therapeutic collagen VII for the disease-challenged skin and relieve specific DEB symptoms",
"Zusammenfassung: Dystrophe epidermolysis bullosa (DEB) ist eine seltene erbliche Hauterkrankung, die durch Mutationen im COL7A1 Gen verursacht wird, welches f\u00fcr das Ankerfibrillenprotein Kollagen VII kodiert. Unter physiologischen Bedingungen vermittelt Kollagen VII Hautstabilit\u00e4t, indem es die oberste Hautschicht, die Epidermis, eng mit der darunterliegenden Schicht, der Dermis, verbindet. Verringerte Mengen oder die komplette Abwesenheit von Kollagen VII in Patienten mit DEB f\u00fchren zu schweren systemischen Krankheitsauspr\u00e4gungen, die neben der Haut auch weitere Organe betreffen. Lebenslange Sensibilit\u00e4t gegen\u00fcber mechanischen Belastungen auf der Haut und Schleimh\u00e4uten, das Auftreten von gro\u00dfen Erosionen an K\u00f6rperoberfl\u00e4chen, die Entstehung von chronischen Wunden und sekund\u00e4rer Fibrose sowie die Entwicklung von aggressivem Hautkrebs sind charakteristische Merkmale der Erkrankung. Die Auswirkungen der Erkrankung beeintr\u00e4chtigen Patienten in ihrer Lebensqualit\u00e4t von fr\u00fchester Kindheit an und sind f\u00fcr die Patienten selbst und deren Angeh\u00f6rige nur schwer zu ertragen. Trotz enormer Anstrengungen seitens der klinischen Forschung und der pharmazeutischen Industrie gibt es gegenw\u00e4rtig keine heilende Therapie f\u00fcr DEB Patienten.Diese Studie beschreibt zum ersten Mal einen Langzeitversuch von lokal injizierten mesenchymalen Stromazellen (MSCs) als Therapieoption f\u00fcr DEB. Humane MSCs aus dem Knochenmark sezernierten in vitro Kollagen VII mit stabilen helikalen Kollagendom\u00e4nen in einem zu humanen Fibroblasten vergleichbarem Ausma\u00df, unabh\u00e4ngig vom Alter oder Geschlecht des einzelnen Spenders und unabh\u00e4ngig von der Passagezahl der MSCs. Wiederholte intradermale Injektionen von MSCs in einem DEB Mausmodell f\u00fchrten zu einer dosis-abh\u00e4ngigen Ablagerung von Kollagen VII an der physiologischen korrekten Stelle, der dermalen-epidermalen Verbindung, wobei MSCs, verglichen mit Fibroblasten, eine bis zu zehnmal h\u00f6here biologische Potenz aufwiesen. MSCs unterst\u00fctzen die Regeneration von DEB Wunden durch die Normalisierung von epidermaler und dermaler Heilung sowie durch Verbesserung der Hautintegrit\u00e4t durch die Neubildung von Ankerfibrill-artigen Strukturen. Zus\u00e4tzlicher Nutzen der MSCs resultierte von ihren anti-inflammatorischen Effekten, der zu einer reduzierten Infiltration von Immunzellen in die verwundete DEB Haut f\u00fchrte. In dieser Studie konnte ein therapeutischer Effekt der Behandlung f\u00fcr mindestens 3 Monate nach ..."
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} | ['gnd:4540403-3', 'gnd:4164652-6', 'gnd:4278308-2'] | ['Kollagen', 'Mesenchymzelle', 'Epidermolysis bullosa dystrophicans'] | ['meda'] | ['Technische Informationsbibliothek (TIB)'] | [['(firstid)BSZ:454100191', '(ppn)846259265', '(contract)FRUB-opus-10509', '(doi)10.6094/UNIFR/10509']] | [''] | [['(classificationName=ddc-dbn)610', '(classificationName=ddc)570', '(classificationName=ddc-dbn)570', '(classificationName=linseach:mapping)meda', '(classificationName=linseach:mapping)bio']] | ['Mesenchymal stromal cell therapy for dystrophic epidermolysis bullosa'] | [['Zusammenfassung: Dystrophic epidermolysis bullosa (DEB) is a rare heritable skin fragility disorder caused by mutations in the COL7A1 gene, coding for the anchoring fibril protein collagen VII. Under physiological conditions the unique function of collagen VII is the mediation of skin stability by attaching the epidermis to the underlying papillary dermis. Reduced amounts or the complete absence of collagen VII in patients with DEB result in severe cutaneous and extra-cutaneous disease manifestations including life-long mechanosensitivity of skin and mucosal surfaces, emergence of large body surface erosions, chronic wounds, secondary fibrosis and development of aggressive squamous cell carcinoma. The consequences of severe DEB massively impede the quality of life from the earliest years on and are hard to bear for the patient and the familiy. Despite tremendous efforts by the scientific community and pharmaceutical companies, no curative therapy is yet available for the treatment of DEB. This study describes the first long-term follow up of locally injected human mesenchymal stromal cells (MSCs) as a therapy option for DEB. Human bone marrow derived MSCs secreted stable triple helical collagen VII in vitro in a comparable amount to human fibroblasts and independent of individual donor age, gender or passage number. Repeated intradermal injections of MSCs in a DEB mouse model resulted in production and deposition of collagen VII at the dermal-epidermal junction, the physiological site of function. The effect was dose dependent with MSCs being up to ten-fold more potent than dermal fibroblasts. MSCs promoted regeneration of DEB wounds via normalization of dermal and epidermal healing and improved skin integrity through de novo formation of anchoring fibril-like structures. Additional benefits were gained by MSCs’ anti-inflammatory effects, which led to decreased immune cell infiltration into injured DEB skin. In this setting the clinical benefit of MSC injections lasted for more than three months.The data from this study suggest that locally applied MSCs are a viable option for DEB therapy, as they provide sufficient amounts of functional therapeutic collagen VII for the disease-challenged skin and relieve specific DEB symptoms', 'Zusammenfassung: Dystrophe epidermolysis bullosa (DEB) ist eine seltene erbliche Hauterkrankung, die durch Mutationen im COL7A1 Gen verursacht wird, welches für das Ankerfibrillenprotein Kollagen VII kodiert. Unter physiologischen Bedingungen vermittelt Kollagen VII Hautstabilität, indem es die oberste Hautschicht, die Epidermis, eng mit der darunterliegenden Schicht, der Dermis, verbindet. Verringerte Mengen oder die komplette Abwesenheit von Kollagen VII in Patienten mit DEB führen zu schweren systemischen Krankheitsausprägungen, die neben der Haut auch weitere Organe betreffen. Lebenslange Sensibilität gegenüber mechanischen Belastungen auf der Haut und Schleimhäuten, das Auftreten von großen Erosionen an Körperoberflächen, die Entstehung von chronischen Wunden und sekundärer Fibrose sowie die Entwicklung von aggressivem Hautkrebs sind charakteristische Merkmale der Erkrankung. Die Auswirkungen der Erkrankung beeinträchtigen Patienten in ihrer Lebensqualität von frühester Kindheit an und sind für die Patienten selbst und deren Angehörige nur schwer zu ertragen. Trotz enormer Anstrengungen seitens der klinischen Forschung und der pharmazeutischen Industrie gibt es gegenwärtig keine heilende Therapie für DEB Patienten.Diese Studie beschreibt zum ersten Mal einen Langzeitversuch von lokal injizierten mesenchymalen Stromazellen (MSCs) als Therapieoption für DEB. Humane MSCs aus dem Knochenmark sezernierten in vitro Kollagen VII mit stabilen helikalen Kollagendomänen in einem zu humanen Fibroblasten vergleichbarem Ausmaß, unabhängig vom Alter oder Geschlecht des einzelnen Spenders und unabhängig von der Passagezahl der MSCs. Wiederholte intradermale Injektionen von MSCs in einem DEB Mausmodell führten zu einer dosis-abhängigen Ablagerung von Kollagen VII an der physiologischen korrekten Stelle, der dermalen-epidermalen Verbindung, wobei MSCs, verglichen mit Fibroblasten, eine bis zu zehnmal höhere biologische Potenz aufwiesen. MSCs unterstützen die Regeneration von DEB Wunden durch die Normalisierung von epidermaler und dermaler Heilung sowie durch Verbesserung der Hautintegrität durch die Neubildung von Ankerfibrill-artigen Strukturen. Zusätzlicher Nutzen der MSCs resultierte von ihren anti-inflammatorischen Effekten, der zu einer reduzierten Infiltration von Immunzellen in die verwundete DEB Haut führte. In dieser Studie konnte ein therapeutischer Effekt der Behandlung für mindestens 3 Monate nach ...']] | ['2015'] | ['http://id.loc.gov/vocabulary/iso639-1/en'] | ['open access'] | [[{'@id': 'gnd:4540403-3'}, {'@id': 'gnd:4164652-6'}, {'@id': 'gnd:4278308-2'}]] | ['gnd:1081264004', 'gnd:4164652-6', 'gnd:4278308-2', 'gnd:4540403-3', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A846259265'] | ['Kühl, Tobias Hans-Jürgen', 'Kollagen', 'Mesenchymzelle', 'Epidermolysis bullosa dystrophicans'] | 18 |
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Repeated intradermal injections of MSCs in a DEB mouse model resulted in production and deposition of collagen VII at the dermal-epidermal junction, the physiological site of function. The effect was dose dependent with MSCs being up to ten-fold more potent than dermal fibroblasts. MSCs promoted regeneration of DEB wounds via normalization of dermal and epidermal healing and improved skin integrity through de novo formation of anchoring fibril-like structures. Additional benefits were gained by MSCs’ anti-inflammatory effects, which led to decreased immune cell infiltration into injured DEB skin. In this setting the clinical benefit of MSC injections lasted for more than three months.The data from this study suggest that locally applied MSCs are a viable option for DEB therapy, as they provide sufficient amounts of functional therapeutic collagen VII for the disease-challenged skin and relieve specific DEB symptoms', 'Zusammenfassung: Dystrophe epidermolysis bullosa (DEB) ist eine seltene erbliche Hauterkrankung, die durch Mutationen im COL7A1 Gen verursacht wird, welches für das Ankerfibrillenprotein Kollagen VII kodiert. Unter physiologischen Bedingungen vermittelt Kollagen VII Hautstabilität, indem es die oberste Hautschicht, die Epidermis, eng mit der darunterliegenden Schicht, der Dermis, verbindet. Verringerte Mengen oder die komplette Abwesenheit von Kollagen VII in Patienten mit DEB führen zu schweren systemischen Krankheitsausprägungen, die neben der Haut auch weitere Organe betreffen. Lebenslange Sensibilität gegenüber mechanischen Belastungen auf der Haut und Schleimhäuten, das Auftreten von großen Erosionen an Körperoberflächen, die Entstehung von chronischen Wunden und sekundärer Fibrose sowie die Entwicklung von aggressivem Hautkrebs sind charakteristische Merkmale der Erkrankung. Die Auswirkungen der Erkrankung beeinträchtigen Patienten in ihrer Lebensqualität von frühester Kindheit an und sind für die Patienten selbst und deren Angehörige nur schwer zu ertragen. Trotz enormer Anstrengungen seitens der klinischen Forschung und der pharmazeutischen Industrie gibt es gegenwärtig keine heilende Therapie für DEB Patienten.Diese Studie beschreibt zum ersten Mal einen Langzeitversuch von lokal injizierten mesenchymalen Stromazellen (MSCs) als Therapieoption für DEB. Humane MSCs aus dem Knochenmark sezernierten in vitro Kollagen VII mit stabilen helikalen Kollagendomänen in einem zu humanen Fibroblasten vergleichbarem Ausmaß, unabhängig vom Alter oder Geschlecht des einzelnen Spenders und unabhängig von der Passagezahl der MSCs. Wiederholte intradermale Injektionen von MSCs in einem DEB Mausmodell führten zu einer dosis-abhängigen Ablagerung von Kollagen VII an der physiologischen korrekten Stelle, der dermalen-epidermalen Verbindung, wobei MSCs, verglichen mit Fibroblasten, eine bis zu zehnmal höhere biologische Potenz aufwiesen. MSCs unterstützen die Regeneration von DEB Wunden durch die Normalisierung von epidermaler und dermaler Heilung sowie durch Verbesserung der Hautintegrität durch die Neubildung von Ankerfibrill-artigen Strukturen. Zusätzlicher Nutzen der MSCs resultierte von ihren anti-inflammatorischen Effekten, der zu einer reduzierten Infiltration von Immunzellen in die verwundete DEB Haut führte. 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} | ['gnd:7544915-8', 'gnd:4290802-4', 'gnd:4013943-8', 'gnd:4160198-1', 'gnd:4191103-9', 'gnd:4006650-2'] | ['Bildung', 'Einstellung', 'Hochschulbildung', 'Zugang', 'Männliche Jugend', 'Migrationshintergrund'] | ['sow'] | [['Technische Informationsbibliothek (TIB)', {'@id': 'gnd:122279956'}, {'@id': 'gnd:122987055'}]] | [['(isbn)3814223314', '(isbn13)9783814223315', '(firstid)GBV:846461390', '(ppn)846461390']] | ['BIS-Verlag der Carl von Ossietzky Universität Oldenburg'] | [['Migrationshintergrund', '(classificationName=linseach:mapping)sow', 'Immigrant youth', '(classificationName=ddc-dbn)300', 'Children of immigrants', '(classificationName=rvk)AL 40700', '(classificationName=rvk)MS 3600', '(classificationName=bk, id=181571374)80.57 - Interkulturelle Erziehung', 'Germany', '(classificationName=ddc)306.430943', '(classificationName=bk, id=106414267)81.21 - Soziale Ungleichheit im Bildungswesen', '(classificationName=ssg)5,3', 'Male immigrants', 'Geschlechterforschung', '(classificationName=rvk)DU 6000', 'Jugend', '(classificationName=bk, id=106412663)71.32 - Mann', '(classificationName=linseach:mapping)pae', '(classificationName=bk, id=106413198)77.56 - Jugendpsychologie', 'Chancengleichheit', 'Education, Higher', '(classificationName=loc)LC3736.G4', 'Bildung']] | ['The underrepresentation of male youth with a migration background in higher education in Germany : "Voices of Society" vs. "Voices of Youth"'] | ['Why are male youth with a migration background underrepresented in higher education in Germany? The answer is not simple and there are certain limitations when developing possible solutions for this problem. This book tries to provide an answer to this question by interviewing male migrant youth and focusing on the already practiced strategies, beliefs and tendencies of those youth who have been successful. The premise is that actual proof is more useful than theoretical proof, is easier to apply and might provide inspiration for other youth. In a way this book is a comparison of qualitative and quantitative research. <engl.>'] | ['2016'] | ['http://id.loc.gov/vocabulary/iso639-1/de'] | ['commercial licence'] | [[{'@id': 'gnd:7544915-8'}, {'@id': 'gnd:4290802-4'}, {'@id': 'gnd:4013943-8'}, {'@id': 'gnd:4160198-1'}, {'@id': 'gnd:4191103-9'}, {'@id': 'gnd:4006650-2'}]] | ['gnd:122279956', 'gnd:122987055', 'gnd:4006650-2', 'gnd:4011882-4', 'gnd:4013943-8', 'gnd:4160198-1', 'gnd:4191103-9', 'gnd:4290802-4', 'gnd:7544915-8', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A846461390'] | ['Melter, Claus', 'Leiprecht, Rudolf', 'Bildung', 'Deutschland', 'Einstellung', 'Hochschulbildung', 'Zugang', 'Männliche Jugend', 'Migrationshintergrund'] | 25 |
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